Synergistic technology for repairing soil and promoting growth of plant stem cells through modified amino active groups

Abstract

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of agricultural technology, and particularly relates to a modified amino active group soil remediation and plant stem cell growth promotion technology. BACKGROUND

[0002] In modern intensive agricultural production, the soil is facing many severe problems. Long-term unreasonable use of chemical fertilizers leads to soil acidification and hardening, soil aggregate structure is destroyed, water and fertilizer retention capacity is significantly reduced, due to long-term use of large amounts of nitrogen fertilizer, the soil pH value is reduced to below 5.5, the soil is seriously hardened, the root growth is greatly hindered, the frequent use of pesticides causes the imbalance of soil microbial community, the number of beneficial microorganisms is sharply reduced, the natural purification and nutrient transformation capacity of the soil is reduced, in addition, industrial pollution leads to excessive content of heavy metals such as cadmium, lead and mercury in the soil, which not only affects the normal growth of plants, but also endangers human health through the food chain. Therefore, the modified amino active group soil remediation and plant stem cell growth promotion technology is proposed. SUMMARY

[0003] The purpose of the present application is to solve the problems existing in the prior art, and a modified amino active group soil remediation and plant stem cell growth promotion technology is provided.

[0004] To achieve the above-mentioned purpose, the present application provides the following technical scheme: The modified amino active group soil remediation and plant stem cell growth promotion technology comprises the following steps: S1: modifying amino acid complex, modifying the active groups in amino acid and fulvic acid, so as to make them synergistically act with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase, catalase, and construct a micro-ecological environment conducive to soil remediation and plant stem cell growth; S2: modifying fulvic acid, modifying fulvic acid by esterification, amidation and other reactions to introduce more active groups, such as carbamoyl group, to enhance its adsorption and preservation capacity for ammonium nitrogen and reduce nitrogen loss; S3: composite enzyme preparation, composite application of cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; S4: trace element chelate, using amino acid as the matrix to chelate trace elements necessary for plant growth such as ferrous glycinate and zinc glutamate, accounting for 3% to 8% of the total mass of the product; S5: carrier and adjuvant, clay minerals such as montmorillonite, kaolin, etc. are selected as carriers to adsorb and release the active ingredients, and appropriate amounts of sodium alginate, polyacrylamide, etc. are added as adjuvants to play the role of water retention, thickening and stabilizing the product; S6: raw material mixing, the prepared modified amino acid complex, modified fulvic acid, complex enzyme preparation, trace element chelate and carrier and adjuvant are added into the mixing equipment according to the formula proportion, and the mixture is made into granular, powdery or liquid product by extrusion granulation or spray granulation; S7: experimental design, a farmland with acidification, hardening and certain heavy metal pollution caused by long-term planting of vegetables is selected, which is divided into multiple plots, each plot area is 30 square meters, and experimental group and control group are set, the experimental group is applied with the soil remediation and plant growth promoting product prepared by the application, and the control group is applied with conventional fertilizer and soil conditioner; S8: product application method, the product obtained in S6 is uniformly applied on the surface of the soil in the experimental group before planting vegetables, and then plowing is carried out to make the product fully mixed with the soil, and the control group is operated according to the conventional fertilization and soil improvement method; S9: experimental monitoring, during the growth period of vegetables, the physical and chemical properties and microbial community changes of the soil are monitored regularly, and the pH value of the soil is measured by using pH meter; S10: experimental results: record the experimental process and obtain the results.

[0005] Preferably, the preparation method of S1 modified amino acid complex is to introduce functional groups such as thiol and carbamoyl groups into basic amino acids such as lysine, glutamic acid, glycine and histidine by chemical grafting reaction, dissolve the basic amino acids in an appropriate amount of organic solvent, add catalyst, slowly drop the reagent containing functional groups under certain temperature and stirring conditions, react for 3-6 hours, and then separate and purify by distillation, extraction, crystallization and other steps to obtain modified amino acid.

[0006] Preferably, the preparation method of S2 modified fulvic acid is to modify the natural fulvic acid by esterification and amidation, and introduce active groups such as carbamoyl groups. Specifically, the natural fulvic acid is subjected to esterification reaction with alcohol under the action of acid catalyst, the reaction temperature is controlled at 60-80℃, the reaction time is 2-4 hours, then the compound containing amino is added for amidation reaction, the reaction temperature is 70-90℃, the reaction time is 3-5 hours, and after the reaction is completed, the modified fulvic acid is obtained through steps such as neutralization, filtration and concentration.

[0007] Preferably, the preparation method of the S3 complex enzyme preparation is to prepare a plurality of enzymes such as cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD, CAT, etc. by specific fermentation process and compounding technology, specifically, the enzyme-producing microbial strains of cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD, CAT, etc. are respectively cultured, such as the cellulase-producing Trichoderma reesei, the urease-producing Bacillus subtilis, the fermentation is carried out under suitable culture medium, temperature and pH value, the fermentation broth is collected after the fermentation is completed, the enzyme protein is extracted through centrifugation, ultrafiltration and other steps, then the enzyme proteins are mixed in a certain proportion, and appropriate protective agents and buffering agents are added to prepare the complex enzyme preparation.

[0008] Preferably, the preparation method of the S4 trace element chelate is to dissolve amino acids in water, adjust the pH value to a suitable range, then slowly add the corresponding trace element salt solution such as ferrous sulfate, zinc sulfate, etc., and react for 1-2 hours under stirring to fully chelate the amino acids and trace elements, and then the trace element chelate is obtained by spray drying or freeze drying.

[0009] Preferably, the product in S8 is uniformly applied on the surface of the soil in the experimental group at a dosage of 300 kg / ha.

[0010] Preferably, the experimental group in S7 selects three different types of soil, namely acidic red soil, neutral loam and alkaline sandy soil, and each type of soil sets an experimental group and a control group, and each group sets 3 repetitions, and each repetition has an area of 20 square meters; Corn is planted in the three types of soil, the product of the application is applied in the experimental group at a dosage of 250 kg / ha before sowing, and the control group applies the local conventional fertilizer and soil conditioner, and the same irrigation, weeding and other field management are carried out during the growth of corn.

[0011] Preferably, at different stages of corn growth, soil samples are collected to analyze their physicochemical properties, including soil pH, nutrient content (nitrogen, phosphorus, potassium, etc.), cation exchange capacity, etc.; and the growth indicators of corn such as plant height, leaf area, biomass, yield, etc. are measured.

[0012] Preferably, in the S9 experimental monitoring process, the potassium dichromate oxidation method is used to determine the content of soil organic matter, the atomic absorption spectrometry is used to determine the content of heavy metals in the soil, the dilution plate method is used to analyze the number of microorganisms such as bacteria, fungi and actinomycetes in the soil, and at the same time, the growth conditions of the vegetables are observed and recorded, including germination rate, plant height, stem diameter, leaf number and size, flowering time, fruit number and weight, etc.

[0013] Compared with the prior art, the modified amino active group soil remediation and plant cell growth promotion technology has the following beneficial effects: 1. The strong chelation of the modified amino active group with heavy metal ions in the soil can effectively reduce the bioavailability and migration of heavy metals, and the chelation removal rate of heavy metals such as cadmium and lead can reach more than 40%, thereby remedying heavy metal contaminated soil; meanwhile, the synergistic effect of the modified fulvic acid and the complex enzyme preparation can improve the soil structure, increase the soil organic matter content, regulate the soil pH, restore the acidified or alkalized soil to the pH range suitable for plant growth, improve the soil water and fertilizer retention capacity, and promote the balance of the soil microbial community and the reproduction of beneficial microorganisms. 2. The synergistic effect of the multiple enzymes promotes the release and transformation of nutrients in the soil, provides sufficient nutrients for plant growth, stimulates the activity of plant stem cells, promotes plant root development, increases the root length and root surface area, enhances the plant's ability to absorb nutrients and water, induces the plant's own disease resistance signal pathway, and improves the plant's stress resistance, such as increasing the plant's survival rate by 20%-30% under drought, saline-alkali and other stress conditions, effectively promoting the growth and development of plants, and improving crop yield and quality. 3. The raw materials of the product are mainly derived from natural amino acids, fulvic acid and enzymes produced by microbial fermentation, which are non-toxic and harmless, do not cause secondary pollution to the soil and environment, and the active ingredients in the product can be naturally degraded in the soil, meeting the requirements of green agriculture and sustainable development. 4. The product formula and preparation process are optimized to adapt to the growth needs of different types of soil and various crops, and good soil remediation and plant growth promotion effects can be achieved for various plants such as acid, neutral and alkaline soils, food crops, economic crops, vegetables and fruit trees, which has a broad application prospect. BRIEF DESCRIPTION OF DRAWINGS

[0014] The accompanying drawings are included to provide a further understanding of the application, and constitute a part of the specification, which together with the embodiments of the application, serve to explain the application, and do not constitute a limitation on the application.

[0015] In the drawings: Figure 1 The modified amino active group soil remediation and plant cell growth promotion technology is proposed. DETAILED DESCRIPTION

[0016] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0017] Embodiment 1: Please refer to Figure 1 The modified amino active group repair soil and promote plant cell growth synergistic technology comprises the following steps: S1: The modified amino acid compound is used to modify the active groups in amino acids and fulvic acid, so that the modified amino acid compound can be synergized with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase to construct a micro-ecological environment conducive to soil repair and plant stem cell growth. The preparation method of the modified amino acid compound is as follows: taking lysine, glutamic acid, glycine and histidine as basic amino acids, functional groups such as thiol and carbamoyl are introduced by chemical grafting reaction, and the content of the functional groups in the product is 15%-25%. Specifically, the basic amino acids are dissolved in an appropriate amount of organic solvent, a catalyst is added, and a reagent containing a functional group such as mercaptoacetic acid or carbamoyl chloride is slowly added under certain temperature and stirring conditions. In the preparation of the modified amino acid, the basic amino acids such as lysine and glutamic acid are dissolved in an organic solvent with a volume ratio of ethanol to water of 4:1 at a specific ratio (for example, lysine:glutamic acid = 3:2), and the concentration of the basic amino acids is controlled at 0.2-0.3 mol / L. Taking lysine as an example, 20% of the mass of lysine is added as a catalyst pyridine, and a reagent containing thiol, such as mercaptoacetic acid, is slowly added under the condition of a stirring rate of 300-400 r / min. The dropping speed is controlled at 1-2 drops per second, and the reaction temperature is maintained at 50-60°C. In this temperature range, the amino group of the amino acid can efficiently react with the carboxyl group of mercaptoacetic acid to form a stable chemical bond. The reaction lasts for 3-4 hours. After the reaction, most of the organic solvent is removed by reduced pressure distillation, and then 3-4 times of extraction with ethyl acetate is performed to remove unreacted raw materials and by-products. Finally, through the recrystallization step, the purity of the thiol-modified amino acid reaches 95%-98%. The reaction lasts for 3-6 hours, and then the modified amino acid is separated and purified through distillation, extraction and crystallization steps. S2: modified fulvic acid, the natural fulvic acid is modified by esterification, amidation and other reactions to introduce more active groups, such as carbamoyl group, to enhance its adsorption and preservation capacity for ammonium nitrogen, reduce nitrogen loss, and the preparation method of modified fulvic acid is to modify the natural fulvic acid by esterification, amidation and other reactions to introduce active groups such as carbamoyl group, which accounts for 10%-20% of the total mass of the product, specifically, the natural fulvic acid is esterified with alcohol (such as ethanol, propanol) under the action of acid catalyst, the reaction temperature is controlled at 60-80℃, and the reaction time is 2-4 hours, then the compound containing amino (such as urea, ethylenediamine) is added for amidation reaction, the reaction temperature is 70-90℃, and the reaction time is 3-5 hours, after the reaction, neutralization, filtration, concentration and other steps are carried out to obtain modified fulvic acid, when preparing modified fulvic acid, the natural fulvic acid is mixed with ethanol at a mass ratio of 1:3, 5% of the mass of fulvic acid is added as a catalyst, the temperature is raised to 70-80℃, the esterification reaction can proceed smoothly at this temperature, and the side reaction can be effectively avoided, after 2.5-3.5 hours of reaction, the esterification process is completed, then urea is added, the mass ratio of fulvic acid to urea is 4:1, the temperature is raised to 80-90℃, and the amidation reaction is carried out, the reaction time is 3.5-4.5 hours, after the reaction, 10%-15% of sodium hydroxide solution is used for neutralization to pH 7.0-7.5, then the insoluble impurities are removed by vacuum filtration, the filtrate is concentrated by rotary evaporation, then freeze-dried to obtain modified fulvic acid, the number of active groups of which is increased by 30%-40% compared with natural fulvic acid; S3: composite enzyme preparation, cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase, catalase are applied together, the preparation method of the composite enzyme preparation is to contain cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD, CAT and other enzymes, and is prepared by specific fermentation process and compounding technology, and accounts for 8%-15% of the total mass of the product, specifically, the enzyme-producing microbial strains of cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD and CAT are respectively cultivated, such as the cellulase-producing Trichoderma reesei and the urease-producing Bacillus subtilis, the fermentation is carried out under the conditions of suitable culture medium, temperature and pH value, for example, when the Trichoderma reesei is cultivated to produce cellulase, the culture medium is selected to be the medium with corn straw powder as carbon source (3%-5% of the mass of the culture medium) and soybean meal powder as nitrogen source (2%-3% of the mass of the culture medium), and a proper amount of inorganic salts such as potassium dihydrogen phosphate (0.1%-0.3%) and magnesium sulfate (0.05%-0.1%) are added, the initial pH value is adjusted to 5.0-5.5, the pH environment is suitable for the growth and enzyme production of the Trichoderma reesei and is beneficial to the efficient expression of the cellulase gene, the cultivation temperature is constant at 28-30°C, the temperature range can ensure the efficient performance of the enzyme reaction in the Trichoderma reesei, maintain the normal metabolism and growth of the cells, and after 72-96 hours of fermentation, the cellulase activity can reach 500-800 IU / mL, for the cultivation of the urease-producing Bacillus subtilis, the culture medium is selected to be the medium with glucose as carbon source (1%-2%) and protein peptone as nitrogen source (1.5%-2.5%), and auxiliary factors such as calcium chloride (0.02%-0.05%) are added, the initial pH value is adjusted to 7.0-7.2, which is close to the optimum pH for the growth of the Bacillus subtilis, the cultivation temperature is set to 37°C, under which the Bacillus subtilis grows and reproduces rapidly and the urease production efficiency is high, after 36-48 hours of cultivation, the urease activity can reach 300-500 U / mL, when the chitinase-producing microorganism is cultivated, the culture medium is constructed by using colloidal chitin as specific carbon source (2%-3%) and yeast powder as nitrogen source (0.5%-1%), the pH value is maintained at 6.0-6.5, the cultivation temperature is 30-32°C, and after 48-72 hours of cultivation, the chitinase activity can reach 200-350 U / mL, and when the beta-glucanase-producing microorganism is cultivated, the culture medium is constructed by using oat beta-glucan as carbon source (1.5%-2.5%) and ammonium sulfate as nitrogen source (0.8%-1.2%), and the pH value is adjusted to 6.5-7.0, temperature control in 32℃- 35℃, fermentation 60 - 80 hours, β-glucanase activity can reach 150 - 250U / mL, in the preparation of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and other oxidation-reduction enzyme, select a variety of nutrient-rich complex medium, such as beef paste (0.3% - 0.5%), protein peptone (1% - 2%), glucose (1% - 1.5%) as the main component, adding trace elements iron, manganese, zinc, etc. (total amount 0.01% -0.03%), culture SOD producing microorganisms, the initial pH value is adjusted to 7.2 - 7.4, temperature maintained at 30℃- 33℃, fermentation 48 - 72 hours, SOD activity can reach 80 - 120 U / mL, POD producing microorganisms culture, pH value is set to 7.0 - 7.2, temperature in 32℃- 35℃, culture 50 - 70 hours, POD activity can reach 100 - 150U / mL, CAT producing microorganisms, the pH value of the culture medium is controlled at 7.3 - 7.5, temperature 30℃- 32℃, after 60- 80 hours of fermentation, CAT activity can reach 120 - 180 U / mL, through the precise control of these conditions, to ensure the efficient output of various enzymes, for the preparation of subsequent complex enzyme preparation, to ensure the best performance of complex enzyme preparation in soil remediation and plant growth process) fermentation end collection of fermentation broth, through centrifugation, ultrafiltration and other steps to extract enzyme protein, then according to certain proportion of various enzyme protein mixed, adding appropriate amount of protective agent (such as glycerol, trehalose) and buffer (such as potassium phosphate - potassium dihydrogen phosphate buffer pair), preparation of complex enzyme preparation. S4: trace element chelate, with amino acid as the base, chelating iron, zinc, manganese, copper, and other trace elements essential for plant growth, such as glycine ferrous, glutamic acid zinc, etc., accounting for 3% - 8% of the total mass of the product. The preparation method of the trace element chelate is to dissolve the amino acid in water, adjust the pH value to the appropriate range (generally 5 - 7), then slowly add the corresponding trace element salt solution, such as ferrous sulfate, zinc sulfate, etc., and react for 1 - 2 hours under stirring conditions to fully chelate the amino acid and trace elements. After the reaction is complete, the trace element chelate is obtained by spray drying or freeze drying. In the preparation of the trace element chelate, taking glycine ferrous as an example, glycine is prepared into a 0.15 - 0.25 mol / L aqueous solution, the pH value is adjusted to 5.5 - 6.0 with dilute hydrochloric acid or sodium hydroxide solution, and ferrous sulfate solution is slowly added. The molar ratio of glycine to ferrous ions is controlled at 2.5:1 - 3:1. The reaction is carried out at a stirring rate of 250 - 350 r / min and a temperature of 40 - 50℃ for 1.5 - 2.5 hours to fully chelate glycine and ferrous ions. After the reaction is complete, the glycine ferrous chelate with good fluidity is obtained by spray drying with the inlet temperature set at 180 - 200℃ and the outlet temperature at 80 - 90℃. The chelation rate reaches 90% - 93% after testing. S5: carrier and auxiliary materials, using montmorillonite, kaolin, and other clay minerals as carriers to adsorb and release active ingredients, and adding appropriate amounts of sodium alginate, polyacrylamide, etc. as auxiliary materials to play the role of water retention, thickening, and stabilizing the product, accounting for 32% - 64% of the total mass of the product; S6: raw material mixing, adding the prepared modified amino acid complex, modified fulvic acid, complex enzyme preparation, trace element chelate, carrier, and auxiliary materials into the mixing equipment according to the formula proportion, and preparing the mixture into granular, powdery, or liquid products by extrusion granulation or spray granulation; S7: experimental design, selecting a farmland with acidification, hardening, and certain heavy metal pollution (cadmium content exceeding the standard) due to long-term planting of vegetables, dividing it into multiple plots, each with an area of 30 square meters, setting up experimental and control groups, applying the soil repair and plant growth promoting product prepared in the invention to the experimental group, and applying conventional fertilizers and soil conditioners to the control group. Three different types of soil are selected for the experimental group, namely acidic red soil (pH value 4.8 - 5.2), neutral loam soil (pH value 6.5 - 7.0), and alkaline sandy soil (pH value 7.8 - 8.2). Each type of soil sets up experimental and control groups, with 3 repetitions for each group, and each repetition has an area of 20 square meters; Corn is planted in three types of soil, the experimental group is applied with the product of the application at a dosage of 250 kg / ha before sowing, the control group is applied with local conventional fertilizer and soil conditioner, during the growth of corn, the same irrigation, weeding and other field management are carried out; At different stages of corn growth, soil samples are collected to analyze their physicochemical properties, including soil pH, nutrient content (nitrogen, phosphorus, potassium, etc.), cation exchange capacity, etc.; at the same time, the growth indicators of corn such as plant height, leaf area, biomass, yield, etc. are measured; Experimental results: in acidic red soil, the soil pH value of the experimental group gradually rises to about 6.0 during the corn growth period, the effective phosphorus content in the soil increases by 28%, the corn yield increases by 20% compared with the control group, in neutral loam soil, the soil water and fertilizer retention capacity is enhanced, the corn root system is more developed, the root activity is increased by 30%, the yield is increased by 15%; in alkaline sandy soil, the soil aggregate structure is improved, the soil bulk density is reduced by 10%, the drought resistance of corn is enhanced, the yield is increased by 18% compared with the control group, the experimental results show that the product of the application can play a good soil repair and plant growth promoting effect in different types of soil; S8: product application method, before planting vegetables, the product obtained by S6 is uniformly applied on the surface of the experimental group soil, the product is uniformly applied on the surface of the experimental group soil at a dosage of 300 kg / ha, then plowing is carried out to make the product fully mixed with the soil, the control group is operated according to the conventional fertilization and soil improvement method; S9: experimental monitoring, during the growth period of vegetables, the physicochemical properties and microbial community changes of soil are regularly monitored, the soil pH value is measured by using pH meter, during the experimental monitoring process, the soil organic matter content is measured by using potassium dichromate oxidation method, the soil heavy metal content is measured by using atomic absorption spectrometry, the number of microorganisms such as bacteria, fungi and actinomycetes in the soil is analyzed by using dilution plate method, at the same time, the growth conditions of vegetables are observed and recorded, including germination rate, plant height, stem diameter, leaf number and size, flowering time, fruit number and weight, etc. Index; S10: experimental results: record the experimental process and obtain the results, in acidic red soil, the soil pH value of the experimental group gradually rises to about 6.0 during the corn growth period, the effective phosphorus content in the soil increases by 28%, the corn yield increases by 20% compared with the control group, in neutral loam soil, the soil water and fertilizer retention capacity is enhanced, the corn root system is more developed, the root activity is increased by 30%, the yield is increased by 15%; in alkaline sandy soil, the soil aggregate structure is improved, the soil bulk density is reduced by 10%, the drought resistance of corn is enhanced, the yield is increased by 18% compared with the control group, the experimental results show that the product of the application can play a good soil repair and plant growth promoting effect in different types of soil.

[0018] Example 2: Please refer to Figure 1 , the modified amino active group repair soil and promote plant cell growth technology, comprising the following steps: S1: modified amino acid complex, the active groups in amino acids and fulvic acid are modified, which are synergized with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase, catalase, to construct a micro-ecological environment conducive to soil repair and plant stem cell growth. The preparation method of the modified amino acid complex is to introduce functional groups such as thiol and carbamoyl into lysine, glutamic acid, glycine, histidine and other basic amino acids, accounting for 21% of the total mass of the product. Specifically, the basic amino acid is dissolved in an appropriate amount of organic solvent, a catalyst is added, and a reagent containing a functional group such as mercaptoacetic acid, carbamoyl chloride, etc. is slowly added under certain temperature and stirring conditions. In the preparation of modified amino acids, the basic amino acids such as lysine and glutamic acid are dissolved in an organic solvent with a volume ratio of ethanol to water of 4:1 at a specific ratio (such as lysine: glutamic acid = 3:2). The concentration of basic amino acid is controlled at 0.3 mol / L. Taking lysine as an example, 20% of its mass of catalyst pyridine is added, and the reagent containing thiol, mercaptoacetic acid, is slowly added under the condition of stirring rate of 350 r / min. The dropping speed is controlled at 1-2 drops per second, and the reaction temperature is maintained at 55°C. Within this temperature range, the amino group of amino acid can efficiently react with the carboxyl group of mercaptoacetic acid to form stable chemical bonds. The reaction time lasts for 3.5 hours. After the reaction is completed, most of the organic solvent is removed by reduced pressure distillation, and then 3 times of extraction with ethyl acetate is used to remove unreacted raw materials and by-products. Finally, through the recrystallization step, the purity of the thiol-modified amino acid reaches 95%. The reaction lasts for 5 hours, and then the modified amino acid is separated and purified by distillation, extraction and crystallization steps. S2: modified fulvic acid, the fulvic acid is modified, more active groups are introduced through esterification, amidation and other reactions, such as carbamoyl group, the adsorption and preservation capacity of ammonium nitrogen is enhanced, the loss of nitrogen is reduced, the preparation method of modified fulvic acid is that the natural fulvic acid is esterified and amidated, active groups such as carbamoyl group are introduced, the content of the product is 15%, specifically, the natural fulvic acid is esterified with alcohol (such as ethanol, propanol) under the action of acid catalyst, the reaction temperature is controlled at 70 DEG C, the reaction time is 2-4 hours, then the compound containing amino (such as urea, ethylenediamine) is added to carry out amidation reaction, the reaction temperature is 80 DEG C, the reaction time is 3-5 hours, after the reaction is completed, neutralization, filtration, concentration and other steps are carried out to obtain modified fulvic acid, when preparing modified fulvic acid, the natural fulvic acid is mixed with ethanol according to the mass ratio of 1:3, 5% of concentrated sulfuric acid is added as catalyst, the temperature is raised to 75 DEG C, the esterification reaction can proceed smoothly at this temperature, and the side reaction can be effectively avoided, after 3 hours of reaction, the esterification process is completed, then urea is added, the mass ratio of fulvic acid to urea is 4:1, the temperature is raised to 85 DEG C, the amidation reaction is carried out, the reaction time is 4 hours, after the reaction is completed, the solution is neutralized to pH 7.0 with 15% sodium hydroxide solution, then the insoluble impurities are removed by vacuum filtration, the filtrate is concentrated by rotary evaporation, then freeze-dried to obtain modified fulvic acid, the number of active groups is increased by 35% compared with natural fulvic acid; S3: composite enzyme preparation, cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase, catalase are applied in combination, the preparation method of the composite enzyme preparation is to contain cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD, CAT and other enzymes, and the composite enzyme preparation is prepared by specific fermentation process and compounding technology, and accounts for 11% of the total mass of the product, and specific microbial strains for producing cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD and CAT are respectively cultivated, such as Trichoderma reesei for producing cellulase, Bacillus subtilis for producing urease, and the like, and the microbial strains are fermented under the conditions of suitable culture medium, temperature and pH value, for example, when Trichoderma reesei is cultivated to produce cellulase, a culture medium with corn straw powder as carbon source (accounting for 4% of the mass of the culture medium) and soybean meal powder as nitrogen source (accounting for 2.5% of the mass of the culture medium) is selected, and a proper amount of inorganic salts such as potassium dihydrogen phosphate (0.2%) and magnesium sulfate (0.05%) are added, and the initial pH value is adjusted to 5.25, the pH environment is suitable for the growth and enzyme production of Trichoderma reesei and the high-efficiency expression of cellulase gene, the cultivation temperature is constant at 30°C, the temperature range can ensure the high-efficiency of enzyme reaction in Trichoderma reesei, and the normal metabolism and growth of cells are maintained, after 84 hours of fermentation, the cellulase activity can reach 650 IU / mL, for the cultivation of Bacillus subtilis for producing urease, a culture medium with glucose as carbon source (1.5%) and protein peptone as nitrogen source (2%) is used, and auxiliary factors such as calcium chloride (0.035%) are added, the initial pH value is adjusted to 7.1, which is close to the optimum pH for the growth of Bacillus subtilis, the cultivation temperature is set to 37°C, under the temperature, Bacillus subtilis grows and reproduces rapidly and has high urease production efficiency, after 36-48 hours of cultivation, the urease activity can reach 400 U / mL, when the microorganism for producing chitinase is cultivated, a culture medium with colloidal chitin as specific carbon source (2.5%) and yeast powder as nitrogen source (0.75%) is constructed, the pH value is maintained at 6.25, and the cultivation temperature is 31°C, after 60 hours of cultivation, the chitinase activity can reach 275 U / mL, and when the microorganism for producing beta-glucanase is cultivated, a culture medium with oat beta-glucan as carbon source (2%) and ammonium sulfate as nitrogen source (1%) is used, the pH value is adjusted to 6.75, and the temperature is controlled at 33.5°C, after 70 hours of fermentation, the beta-glucanase activity can reach 20 U / mL, when the oxidoreductases such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) are prepared, a composite culture medium rich in various nutrients is selected, such as a culture medium with beef extract (0.4%), protein peptone (1.5%) and glucose (1.25%) as main components, and trace elements iron, manganese and zinc (total amount 0.02% and the initial pH value is adjusted to 7.3 when the SOD-producing microorganism is cultured, and the temperature is maintained at 31.5°C, and the SOD activity can reach 100 U / mL after 60 hours of fermentation. When the POD-producing microorganism is cultured, the pH value is set to 7.1, the temperature is 33.5°C, and the POD activity can reach 125 U / mL after 60 hours of culture. When the CAT-producing microorganism is cultured, the pH value of the culture medium is controlled at 7.4, the temperature is 31°C, and the CAT activity can reach 160 U / mL after 70 hours of fermentation. Through precise control of these conditions, the efficient production of various enzymes is ensured, laying a foundation for the subsequent preparation of complex enzyme preparations and ensuring that the complex enzyme preparations can play the best performance in soil remediation and plant growth promotion. S4: Trace element chelate, using amino acid as the matrix, chelating iron, zinc, manganese, and copper, which are essential trace elements for plant growth, such as glycine ferrous and glutamic acid zinc, accounting for 3%-8% of the total mass of the product. The preparation method of the trace element chelate is as follows: dissolving the amino acid in water, adjusting the pH value to the appropriate range (generally 6), then slowly adding the corresponding trace element salt solution such as ferrous sulfate and zinc sulfate, and reacting for 1-2 hours under stirring conditions to fully chelate the amino acid and trace elements. After the reaction is completed, the trace element chelate is obtained by spray drying or freeze drying. In the preparation of the trace element chelate, taking glycine ferrous as an example, glycine is prepared into a 0.2 mol / L aqueous solution, the pH value is adjusted to 5.75 with dilute hydrochloric acid or sodium hydroxide solution, and ferrous sulfate solution is slowly added. The molar ratio of glycine to ferrous ion is controlled at 2.5:1. The reaction is carried out at a stirring rate of 300 r / min and a temperature of 45°C for 2 hours to fully chelate glycine and ferrous ion. After the reaction is completed, the glycine ferrous chelate with good fluidity is obtained by spray drying with the inlet temperature set at 190°C and the outlet temperature at 85°C. The chelation rate reaches 93% after detection. S5: Carrier and adjuvant, using montmorillonite, kaolin, and other clay minerals as carriers to adsorb and release active ingredients, and adding appropriate amounts of sodium alginate and polyacrylamide as adjuvants to play the roles of water retention, thickening, and product stabilization, accounting for 48% of the total mass of the product. S6: Raw material mixing, adding the prepared modified amino acid complex, modified fulvic acid, complex enzyme preparation, trace element chelate, carrier, and adjuvant into a mixing device according to the formula ratio, and preparing the mixture into a granular, powdery, or liquid product by extrusion granulation or spray granulation. S7: Experimental design, select a piece of farmland due to long-term planting of vegetables leading to soil acidification, hardening and the presence of some heavy metal pollution (cadmium content exceeds the standard), divide it into multiple plots, each plot area is 30 square meters, set up experimental group and control group, the experimental group applies the soil remediation and plant growth promoting product prepared by the application, the control group applies conventional fertilizer and soil conditioner, the test group selects three different types of soil, which are acid red soil (pH value 5), neutral loam (pH value 6.75) and alkaline sandy soil (pH value 8), each type of soil sets up experimental group and control group, each group sets up 3 repeats, and each repeat area is 20 square meters; Plant corn in three types of soil, the experimental group applies the product of the application at a dosage of 250 kg / ha before sowing, and the control group applies local conventional fertilizer and soil conditioner, during the growth of corn, the same irrigation, weeding and other field management are carried out; At different stages of corn growth, soil samples are collected to analyze their physicochemical properties, including soil pH, nutrient content (nitrogen, phosphorus, potassium, etc.), cation exchange capacity, etc.; at the same time, the growth indicators of corn such as plant height, leaf area, biomass, yield, etc. are measured; Experimental results: in acid red soil, the soil pH value of the experimental group gradually rises to about 6.0 during the corn growth period, the effective phosphorus content in the soil increases by 28%, and the corn yield increases by 20% compared with the control group; in neutral loam, the soil water and fertilizer retention capacity is enhanced, the corn root system is more developed, the root activity is increased by 30%, and the yield is increased by 15%; in alkaline sandy soil, the soil aggregate structure is improved, the soil bulk density is reduced by 10%, the drought resistance of corn is enhanced, and the yield is increased by 18% compared with the control group; the experimental results show that the product of the application can play a good soil remediation and plant growth promoting role in different types of soil; S8: Product application method, before planting vegetables, the product obtained in S6 is uniformly applied on the surface of the experimental group soil, the product is uniformly applied on the surface of the experimental group soil at a dosage of 300 kg / ha, then plowing is carried out to mix the product with the soil, and the control group is operated according to the conventional fertilization and soil improvement method; S9: Experimental monitoring, during the growth period of vegetables, the physicochemical properties and microbial community changes of the soil are monitored regularly, the soil pH value is measured by pH meter, the soil organic matter content is measured by potassium dichromate oxidation method, the soil heavy metal content is measured by atomic absorption spectrometry, the number of bacteria, fungi, actinomycetes and other microorganisms in the soil is analyzed by dilution plate method, at the same time, the growth conditions of vegetables are observed and recorded, including germination rate, plant height, stem diameter, leaf number and size, flowering time, fruit number and weight, etc. indicators; S10: Experimental results: record the experiment process and get the results, in the acidic red soil, the experimental group soil pH value gradually rises to about 6.1 in the corn growth cycle, the effective phosphorus content in soil increases by 30%, the corn yield is increased by 18% than the control group, in neutral loam, the soil water and fertilizer retention capacity is enhanced, the corn root system is more developed, the root activity is increased by 28%, the yield is increased by 14%; in alkaline sandy soil, the soil aggregate structure is improved, the soil bulk density is reduced by 9%, the drought resistance of corn is enhanced, the yield is increased by 20% than the control group, the experimental results show that the product can play a good soil repair and plant growth promoting effect in different types of soil.

[0019] Example 3: Please refer to Figure 1 , the modified amino active group repair soil and promote plant stem cell growth technology, comprising the following steps: S1: modified amino acid compound, the active groups in amino acid and fulvic acid are modified, which cooperates with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase, catalase to construct a micro-ecological environment conducive to soil repair and plant stem cell growth, the preparation method of the modified amino acid compound is that functional groups such as thiol and carbamoyl are introduced into basic amino acids such as lysine, glutamic acid, glycine and histidine by chemical grafting reaction, which accounts for 25% of the total mass of the product, specifically, the basic amino acids are dissolved in an appropriate amount of organic solvent, a catalyst is added, and a reagent containing a functional group such as mercaptoacetic acid and carbamoyl chloride is slowly added under certain temperature and stirring conditions, in the preparation of modified amino acids, the basic amino acids such as lysine and glutamic acid are dissolved in an organic solvent with a volume ratio of ethanol to water of 4:1 at a specific ratio (such as lysine: glutamic acid = 3:2), the concentration of basic amino acids is controlled at 0.3 mol / L, taking lysine as an example, 20% of its mass of catalyst pyridine is added, the stirring speed is 300-400 r / min, the thiol-containing reagent mercaptoacetic acid is slowly added at a speed of 1-2 drops per second, and the reaction temperature is maintained at 60°C, in this temperature range, the amino group of amino acid can efficiently react with the carboxyl group of mercaptoacetic acid to form stable chemical bonds, the reaction time lasts for 4 hours, after the reaction, most of the organic solvent is removed by reduced pressure distillation, then 3-4 times of ethyl acetate extraction is used to remove unreacted raw materials and by-products, finally, the purity of the thiol-modified amino acid reaches 95%-98% after the recrystallization step, the reaction lasts for 5 hours, then the modified amino acid is separated and purified by distillation, extraction and crystallization steps; S2: modified fulvic acid, the fulvic acid is modified, more active groups are introduced through esterification, amidation and other reactions, such as carbamoyl group, the adsorption and preservation capacity of ammonium nitrogen is enhanced, the loss of nitrogen is reduced, the preparation method of modified fulvic acid is that the natural fulvic acid is esterified and amidated, active groups such as carbamoyl group are introduced, the content of the product is 15%, specifically, the natural fulvic acid is esterified with alcohol (such as ethanol, propanol) under the action of acid catalyst, the reaction temperature is controlled at 70 DEG C, the reaction time is 3 hours, then the compound containing amino (such as urea, ethylenediamine) is added to carry out amidation reaction, the reaction temperature is 80 DEG C, the reaction time is 4 hours, after the reaction is completed, the modified fulvic acid is obtained through the steps of neutralization, filtration, concentration and the like, when preparing the modified fulvic acid, the natural fulvic acid is mixed with ethanol according to the mass ratio of 1:3, 5% of concentrated sulfuric acid is added as catalyst, the temperature is raised to 80 DEG C, the esterification reaction can proceed smoothly at this temperature, at the same time, the side reaction can be effectively avoided, after 3.5 hours of reaction, the esterification process is completed, then urea is added, the mass ratio of fulvic acid to urea is 4:1, the temperature is raised to 80 DEG C, the amidation reaction is carried out, the reaction time is 4.5 hours, after the reaction is completed, the solution is neutralized to pH 7.0 with 10% sodium hydroxide solution, then the insoluble impurities are removed by vacuum filtration, the filtrate is concentrated by rotary evaporation, then freeze-dried to obtain the modified fulvic acid, the number of active groups is increased by 40% compared with the natural fulvic acid; S3: composite enzyme preparation, cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase, catalase are applied together, the preparation method of the composite enzyme preparation is to contain cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD, CAT and other enzymes, and is prepared by specific fermentation process and compounding technology, and accounts for 15% of the total mass of the product, specifically, the enzyme-producing microbial strains of cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD and CAT are respectively cultivated, such as the cellulase-producing Trichoderma reesei, the urease-producing Bacillus subtilis, the fermentation is carried out under the conditions of suitable culture medium, temperature and pH value, for example, when the Trichoderma reesei is cultivated to produce cellulase, the culture medium is selected, corn straw powder is used as carbon source (5% of the mass of the culture medium), soybean meal powder is used as nitrogen source (2% of the mass of the culture medium), a proper amount of inorganic salt such as potassium dihydrogen phosphate (0.1%) and magnesium sulfate (0.1%) is added, the initial pH value is adjusted to 5.0, the pH environment is suitable for the growth and enzyme production of the Trichoderma reesei and is beneficial to the high-efficiency expression of the cellulase gene, the cultivation temperature is constant at 30 DEG C, the temperature range can ensure the high-efficiency of the enzyme reaction in the Trichoderma reesei, and the normal metabolism and growth of the cells are maintained, after 96 hours of fermentation, the cellulase activity can reach 500 IU / mL, for the cultivation of the Bacillus subtilis to produce urease, the culture medium is prepared by using glucose as carbon source (2%) and protein peptone as nitrogen source (1.5%), auxiliary factors such as calcium chloride (0.02%) are added, the initial pH value is adjusted to 7.2, which is close to the optimum pH for the growth of the Bacillus subtilis, the cultivation temperature is set to 37 DEG C, under the temperature, the Bacillus subtilis grows and reproduces rapidly and has high urease production efficiency, after 36 hours of cultivation, the urease activity can reach 300 U / mL, when the chitinase-producing microorganism is cultivated, the culture medium is prepared by using colloidal chitin as specific carbon source (3%) and yeast powder as nitrogen source (0.5%), the pH value is maintained at 6.5, the cultivation temperature is 32 DEG C, after 48 hours of cultivation, the chitinase activity can reach 200 U / mL, when the beta-glucanase-producing microorganism is cultivated, the culture medium is prepared by using oat beta-glucan as carbon source (1.5%) and ammonium sulfate as nitrogen source (1.2%), the pH value is adjusted to 6.5, the temperature is controlled at 35 DEG C, after 60 hours of fermentation, the beta-glucanase activity can reach 200 U / mL, when the oxidoreductases such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) are prepared, the composite culture medium rich in various nutrients is selected, such as beef extract (0.5%), protein peptone (2%) and glucose (1%), trace elements such as iron, manganese and zinc (total amount 0.01%) are added, when the SOD-producing microorganism is cultivated, the initial pH value is adjusted to 7.4, the SOD activity can reach 80 U / mL, the pH value of the culture medium is set to 7.0, the temperature is 35℃, and the culture is carried out for 70 hours, and the POD activity can reach 100 U / mL, the culture medium for producing CAT is controlled at a pH value of 7.5, the temperature is 30℃, and the culture is carried out for 80 hours, and the CAT activity can reach 120 U / mL, through accurate control of these conditions, the efficient production of various enzymes is ensured, and a foundation is laid for the preparation of subsequent complex enzyme preparations, and the best performance of the complex enzyme preparation in soil remediation and plant growth promotion is ensured. After fermentation, the fermentation broth is collected, enzyme proteins are extracted through centrifugation, ultrafiltration and other steps, then various enzyme proteins are mixed according to a certain proportion, and appropriate protective agents (such as glycerol and trehalose) and buffer agents (such as potassium phosphate-dipotassium phosphate buffer pair) are added to prepare the complex enzyme preparation. S4: trace element chelate, amino acid as matrix, chelating iron, zinc, manganese, copper, plant growth essential trace elements such as glycine ferrous, zinc glutamate, etc., accounting for 3%-8% of the total mass of the product, the preparation method of trace element chelate is to dissolve amino acid in water, adjust the pH value to the appropriate range (generally 7), then slowly add the corresponding trace element salt solution such as ferrous sulfate, zinc sulfate, etc., under stirring for 1-2 hours to fully chelate amino acid and trace elements, after the reaction is completed, trace element chelate is obtained by spray drying or freeze drying, taking glycine ferrous as an example, glycine is prepared into a 0.15 mol / L aqueous solution, the pH value is adjusted to 6.0 with dilute hydrochloric acid or sodium hydroxide solution, ferrous sulfate solution is slowly added, the molar ratio of glycine to ferrous ion is controlled at 2.5:1, the reaction is carried out under the conditions of stirring rate 350 r / min and temperature 40℃ for 1.5 hours to fully chelate glycine and ferrous ion, after the reaction is completed, the glycine ferrous chelate with good fluidity is obtained by spray drying, the inlet temperature is set to 200℃, and the outlet temperature is 80℃, the chelation rate reaches 93% through detection; S5: carrier and adjuvant, clay minerals such as montmorillonite and kaolin are selected as carriers to adsorb and release active ingredients, and appropriate amounts of sodium alginate and polyacrylamide are added as adjuvants to play the role of water retention, thickening and stabilizing the product, accounting for 55% of the total mass of the product; S6: raw material mixing, the prepared modified amino acid complex, modified fulvic acid, complex enzyme preparation, trace element chelate, carrier and adjuvant are added to the mixing equipment according to the formula proportion, and the mixed materials are prepared into granular, powdery or liquid products by extrusion granulation or spray granulation; S7: Experimental design, select a piece of farmland due to long-term planting of vegetables leading to soil acidification, hardening and the presence of some heavy metal pollution (cadmium content exceeds the standard), divide it into multiple plots, each plot area is 30 square meters, set up experimental group and control group, the experimental group applies the soil remediation and plant growth promoting product prepared by the application, the control group applies conventional fertilizer and soil conditioner, the test group selects three different types of soil, which are acid red soil (pH value 5), neutral loam (pH value 7.0) and alkaline sandy soil (pH value 7.8), each type of soil sets experimental group and control group, each group sets 3 repeats, each repeat area is 20 square meters; Plant corn in three types of soil, the experimental group applies the product of the application at a dosage of 250 kg / ha before sowing, the control group applies local conventional fertilizer and soil conditioner, during the growth of corn, the same irrigation, weeding and other field management are carried out; At different stages of corn growth, soil samples are collected to analyze their physicochemical properties, including soil pH, nutrient content (nitrogen, phosphorus, potassium, etc.), cation exchange capacity, etc.; at the same time, the growth indicators of corn such as plant height, leaf area, biomass, yield, etc. are measured; Experimental results: in acid red soil, the soil pH value of the experimental group gradually rises to about 6.0 during the corn growth period, the effective phosphorus content in the soil increases by 28%, the corn yield increases by 20% compared with the control group; in neutral loam, the soil water and fertilizer retention capacity is enhanced, the corn root system is more developed, the root activity is increased by 30%, and the yield is increased by 15%; in alkaline sandy soil, the soil aggregate structure is improved, the soil bulk density is reduced by 10%, the drought resistance of corn is enhanced, and the yield is increased by 18% compared with the control group; the experimental results show that the product of the application can play a good soil remediation and plant growth promoting role in different types of soil; S8: Product application method, before planting vegetables, the product obtained in S6 is uniformly applied on the surface of the experimental group soil, the product is uniformly applied on the surface of the experimental group soil at a dosage of 300 kg / ha, then plowing is carried out to mix the product with the soil, the control group is operated according to the conventional fertilization and soil improvement method; S9: Experimental monitoring, during the growth period of vegetables, the physicochemical properties and microbial community changes of the soil are monitored regularly, the soil pH value is measured by pH meter, the soil organic matter content is measured by potassium dichromate oxidation method, the soil heavy metal content is measured by atomic absorption spectrometry, the number of bacteria, fungi, actinomycetes and other microorganisms in the soil is analyzed by dilution plate method, at the same time, the growth conditions of vegetables are observed and recorded, including germination rate, plant height, stem diameter, leaf number and size, flowering time, fruit number and weight, etc. indicators; S10: Experimental results: record the experiment process and get the results, in the acidic red soil, the experimental group soil pH value gradually rises to about 5.85 in the corn growth cycle, the effective phosphorus content in soil increases by 27%, the corn yield is increased by 21% than the control group, in neutral loam, the soil water and fertilizer retention capacity is enhanced, the corn root system is more developed, the root activity is increased by 28%, the yield is increased by 14%; in alkaline sandy soil, the soil aggregate structure is improved, the soil bulk density is reduced by 11%, the drought resistance of corn is enhanced, the yield is increased by 17% than the control group, the experimental results show that the product can play a good soil repair and plant growth promoting effect in different types of soil.

[0020] The application modifies the active groups in amino acids and fulvic acid, so that they synergize with various enzymes to construct a micro-ecological environment conducive to soil repair and plant stem cell growth. Specifically, a specific functional group is introduced on the amino group of the amino acid by chemical modification means to enhance the chelation ability of the amino acid with heavy metal ions in the soil and the buffering capacity of the soil pH value. For example, a strong complexing thiol (-SH) is grafted to the amino group of the amino acid to form a modified amino acid, and the chelation constant of the modified amino acid for cadmium ions is increased by 2.5 orders of magnitude than that of ordinary amino acids. Fulvic acid, as the component with the smallest molecular weight and the highest activity in humic acid, contains carboxyl, phenolic hydroxyl and other functional groups, and has certain complexing, chelating and surface adsorption capacity. The application further modifies the fulvic acid by esterification, amidation and other reactions to introduce more active groups, such as carbamoyl (-CONH2), to enhance its adsorption and preservation capacity for ammonium nitrogen and reduce nitrogen loss. Meanwhile, the application complexly applies various enzymes, such as cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and the like. Cellulase and hemicellulase can accelerate the decomposition of organic matters such as straw and stubble in the soil to transform into humus, increase the content of soil organic matter and improve the soil structure. In the straw field experiment after adding the complex enzyme, the decomposition rate is increased by 45%. Urease and phosphatase can decompose the insoluble nitrogen and phosphorus compounds in the soil to release the nutrients absorbable by plants. In the phosphorus-deficient soil, the effective phosphorus content is increased by more than 22% after using the complex enzyme. Chitinase and beta-glucanase can induce the plant disease resistance signal pathway to improve the plant disease resistance. SOD, POD and CAT can help to remove the free radicals generated by the plant due to the stress to reduce the oxidative damage. After using the complex enzyme in saline-alkali land, the survival rate of crops is increased by 25%.

[0021] High-efficiency soil remediation: Through the strong chelation of modified amino active groups with heavy metal ions in the soil, the bioavailability and mobility of heavy metals can be effectively reduced. The chelation removal rate of heavy metals such as cadmium and lead can reach more than 40%, which can remediate heavy metal contaminated soil. At the same time, the synergistic effect of modified fulvic acid and complex enzyme preparation can improve soil structure, increase soil organic matter content, regulate soil pH, make acidified or alkalized soil gradually restore to the suitable pH range for plant growth, improve soil water and fertilizer retention capacity, and promote the balance of soil microbial community and the reproduction of beneficial microorganisms.

[0022] Significant plant growth promotion: The synergistic effect of multiple enzymes promotes the release and transformation of nutrients in the soil, providing sufficient nutrients for plant growth. Modified amino acids and trace element chelates can stimulate the activity of plant stem cells, promote root development, increase root length and root surface area, and enhance the plant's ability to absorb nutrients and water. At the same time, it induces the plant's own disease resistance signaling pathway, improves the plant's stress resistance, and increases the plant's survival rate by 20%-30% under drought, saline-alkali and other adverse conditions. It effectively promotes plant growth and development, improves crop yield and quality.

[0023] Environmentally friendly and sustainable: The raw materials of the product are mainly derived from natural amino acids, fulvic acid and enzymes produced by microbial fermentation, which are non-toxic and harmless, and will not cause secondary pollution to the soil and environment. The active ingredients in the product can be naturally degraded in the soil, meeting the requirements of green agriculture and sustainable development.

[0024] Wide applicability: The product formula and preparation process have been optimized to meet the growth needs of different types of soil and various crops. Whether it is acidic, neutral or alkaline soil, as well as grain crops, economic crops, vegetables, fruit trees and other types of plants, it can achieve good soil remediation and plant growth promotion effect, and has broad application prospects.

Claims

1. Modified amino active group repair soil and promote plant cell growth synergistic technology, characterized by: Comprise the following steps: S1: modified amino acid complex, the active groups in amino acid and fulvic acid are modified, so that it is synergized with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase, catalase, to build a micro-ecological environment conducive to soil repair and plant stem cell growth; S2: modified fulvic acid, modify fulvic acid, introduce more active groups through esterification, amidation and other reactions, such as introducing carbamoyl group, enhancing its adsorption and preservation capacity to ammonium nitrogen, reducing nitrogen loss; S3: complex enzyme preparation, cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase, catalase are applied; S4: trace element chelate, with amino acid as matrix, chelating iron, zinc, manganese, copper, plant growth essential trace elements, such as glycine ferrous, glutamic acid zinc, accounting for 3% - 8% of the total mass of the product; S5: carrier and auxiliary material, select montmorillonite, kaolin and other clay minerals as carrier, adsorb and slow release active ingredients, at the same time add appropriate amount of sodium alginate, polyacrylamide and other auxiliary materials, play the role of water retention, thickening and stabilizing the product; S6: raw material mixing, the prepared modified amino acid complex, modified fulvic acid, complex enzyme preparation, trace element chelate and carrier and auxiliary material are added into the mixing equipment according to the formula proportion, the mixture is prepared into granular, powdery or liquid product by extrusion granulation or spray granulation; S7: experimental design, select a farmland which is acidified, cemented and has certain heavy metal pollution due to long-term planting of vegetables, divide it into multiple plots, each plot area is 30 square meters, set up experimental group and control group, the experimental group applies the soil repair and plant growth promoting product prepared by the application, the control group applies conventional fertilizer and soil conditioner; S8: product application method, before planting vegetables, the product obtained in S6 is uniformly applied on the surface of the soil in the experimental group, then ploughing is carried out, so that the product is fully mixed with the soil, the control group is operated according to the conventional fertilization and soil improvement method; S9: experimental monitoring, during the growth period of vegetables, the physical and chemical properties of soil and the change of microbial community are monitored regularly, and the pH value of soil is measured by pH meter; S10: experimental results: record the experimental process and get the results.

2. The modified amino active group repair soil and promote plant cell growth synergistic technology according to claim 1, characterized in that: The preparation method of S1 modified amino acid complex is to take lysine, glutamic acid, glycine, histidine and the like as basic amino acid, introduce functional groups such as mercapto and carbamoyl through chemical grafting reaction, dissolve the basic amino acid in a proper amount of organic solvent, add catalyst, slowly drop the reagent containing functional groups under certain temperature and stirring condition, react for 3 - 6 hours, then separate and purify by distillation, extraction, crystallization and the like steps to obtain modified amino acid.

3. The modified amino active group repair soil and promote plant cell growth technology according to claim 1, characterized in that: The preparation method of the S2 modified fulvic acid is to modify the natural fulvic acid by esterification and amidation, and introduce active groups such as carbamoyl groups. Specifically, the natural fulvic acid is subjected to esterification reaction with alcohol under the action of an acidic catalyst, the reaction temperature is controlled at 60-80 DEG C, the reaction time is 2-4 hours, then a compound containing amino is added for amidation reaction, the reaction temperature is 70-90 DEG C, the reaction time is 3-5 hours, and after the reaction is completed, the modified fulvic acid is obtained through steps of neutralization, filtration and concentration.

4. The modified amino active group repair soil and promote plant cell growth technology according to claim 1, characterized in that: The preparation method of the S3 composite enzyme preparation is to prepare a plurality of enzymes including cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD and CAT through specific fermentation process and compounding technology. Specifically, the enzyme-producing microbial strains of cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, SOD, POD and CAT are cultured respectively, such as the cellulase-producing Trichoderma reesei and the urease-producing Bacillus subtilis. The fermentation is carried out under suitable culture medium, temperature and pH value. After the fermentation is completed, the fermentation liquor is collected, and the enzyme protein is extracted through steps of centrifugation and ultrafiltration. Then, the enzyme proteins are mixed in a certain proportion, and a proper amount of protective agent and buffer agent is added to prepare the composite enzyme preparation.

5. The modified amino active group repair soil and promote plant cell growth technology according to claim 1, characterized in that: The preparation method of the S4 trace element chelate is to dissolve amino acids in water, adjust the pH value to a suitable range, then slowly add the corresponding trace element salt solution such as ferrous sulfate and zinc sulfate, and react for 1-2 hours under stirring to fully chelate the amino acids and trace elements. The trace element chelate is obtained by spray drying or freeze drying after the reaction is completed.

6. The modified amino active group repair soil and growth promoting plant cell synergistic technology according to claim 1, characterized in that: The S8 product is uniformly applied on the surface of the soil in the experimental group at a dosage of 300 kg / ha.

7. The modified amino active group repair soil and promote plant cell growth technology according to claim 1, characterized in that: The S7 experimental group selects three different types of soil, namely acidic red soil, neutral loam and alkaline sandy soil. Each type of soil is set with an experimental group and a control group, and each group is set with 3 repetitions, with an area of 20 square meters for each repetition. Corn is planted in the three types of soil. The product of the application is applied at a dosage of 250 kg / ha in the experimental group before sowing, and the control group is applied with local conventional fertilizer and soil conditioner. During the growth of corn, the same field management such as irrigation and weeding is carried out.

8. The modified amino active group repair soil and growth promoting plant cell synergistic technology according to claim 7, characterized in that: At different stages of corn growth, soil samples are collected to analyze the physicochemical properties, including soil pH, nutrient content (nitrogen, phosphorus, potassium, etc.), cation exchange capacity, etc. Meanwhile, the growth indicators of corn such as plant height, leaf area, biomass and yield are measured.

9. The modified amino active group repair soil and growth promoting plant cell synergistic technology according to claim 1, characterized in that: In the S9 experimental monitoring process, the potassium dichromate oxidation method is used to determine the content of soil organic matter, the atomic absorption spectrometry is used to determine the content of heavy metals in the soil, the dilution plate method is used to analyze the number of microorganisms such as bacteria, fungi and actinomycetes in the soil, and the growth conditions of vegetables are observed and recorded, including germination rate, plant height, stem diameter, leaf number and size, flowering time, fruit number and weight, etc.

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