Efficient licochalcone A purification method based on cyclodextrin inclusion-dissociation

Abstract

The invention relates to the technical field of separation and purification, and provides an efficient licochalcone A purification method based on cyclodextrin inclusion-dissociation, which comprises the following steps: S1, extracting liquorice with ethanol, and concentrating an extracting solution to obtain a concentrated solution; s2, extracting the concentrated solution with ethyl acetate to obtain a concentrated organic layer; s3, carrying out inclusion and reverse-phase extraction on the concentrated organic layer by using a cyclodextrin aqueous solution to obtain a cyclodextrin inclusion water layer; s4, promoting dissociation of the cyclodextrin inclusion water layer through sodium chloride and heating, and extracting through ethyl acetate to obtain licochalcone A coarse crystals; and S5, recrystallizing with ethanol and decolorizing with activated carbon to obtain licochalcone A. In the purification process of the licochalcone A, the ethanol, the ethyl acetate and the water are used as solvents, so that the use of toxic forbidden reagents is abandoned, and the production cost is effectively reduced while the process flow is simplified. In addition, cyclodextrin and sodium chloride can be recycled in the production process, so that the production of licochalcone A is more economical and environment-friendly.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of separation and purification, and particularly relates to a high-efficiency purification method for glycyrrhiza chalcone A based on cyclodextrin inclusion-dissociation. BACKGROUND

[0002] Glycyrrhiza chalcone A is a natural flavonoid compound extracted from plants of the genus Glycyrrhiza, which belongs to chalcone derivatives. Its chemical structure is connected by two aromatic rings through alpha, beta-unsaturated ketone, has unique biological activity, and has multiple pharmacological activities such as anti-inflammatory, antioxidant, antitumor, antibacterial, antiparasitic, and acne-removing.

[0003] At present, the extraction and separation and purification of glycyrrhiza chalcone A mostly use column chromatography methods. For example, Chinese patent CN1101117311 A relates to a preparation method of high-purity glycyrrhiza chalcone A. The process steps are as follows: the glycyrrhiza is crushed and then extracted with a methanol-water, ethanol-water or acetone-water system. The extract is concentrated and then extracted with any one of n-butanol, ethyl acetate, n-hexane, chloroform or isoamyl alcohol. The extraction phase is concentrated and then subjected to column chromatography separation. The column chromatography filler used is a macroporous resin, a polyamide or a dextran gel. The eluent is composed of an ethanol aqueous solution or a methanol aqueous solution or an acetone aqueous solution. Gradient system is performed. The high-concentration section eluate is collected. After concentration and drying, glycyrrhiza chalcone A is obtained by dissolution and crystallization. Chinese patent CN105859538B relates to a purification method of glycyrrhiza chalcone A. The process steps are as follows: the glycyrrhiza is crushed and then subjected to reflux extraction with one or more solvents selected from methanol, ethanol, ethyl formate, ethyl acetate, dichloromethane, dioxane and acetone. The extract is concentrated and then subjected to separation by column chromatography. The column layer filler selected is silica gel. The elution solvent is divided into two sections. The first solvent is selected from one or more of hexane, octane, benzene, toluene and xylene. The second solvent is selected from one or more of methanol, ethanol, ethyl formate, dichloromethane and dioxane. After the column chromatography liquid is collected and concentrated, crystallization is performed. The crystallization solvent is selected from one or more of methanol, ethanol, acetone, dioxane, tetrahydrofuran, pyridine and dimethylformamide mixed with water. It can be seen that the conventional extraction method has poor selectivity and insufficient product purity. When the traditional column chromatography method is used to improve the purity, there are problems such as long operation period, large solvent consumption, high equipment cost and difficulty in scaling up. Meanwhile, the above methods have complex solvent systems, and most of them use strong toxic reagents such as acetone, n-hexane, cyclohexane, tetrahydrofuran, dioxane and dichloromethane, which are prohibited ingredients in the cosmetic safety technical specification, and there is a great safety risk of solvent residue.

[0004] Therefore, it is urgent to develop a safe and simple glycyrrhiza chalcone A high-efficiency purification method, which can improve the extraction efficiency, reduce the cost and environmental pollution while ensuring high purity of glycyrrhiza chalcone A. SUMMARY

[0005] In order to solve the above technical problems, the present application aims to provide a high-efficiency purification method of glycyrrhizic chalcone A based on cyclodextrin inclusion-dissociation.

[0006] In order to achieve the above-mentioned purposes, the technical scheme adopted by the present application is as follows:

[0007] The present application provides a high-efficiency purification method of glycyrrhizic chalcone A based on cyclodextrin inclusion-dissociation, comprising the following steps:

[0008] S1. After drying licorice, it is crushed and extracted 2-3 times with 60wt%-95wt% ethanol aqueous solution, and the extract is combined and concentrated to obtain a concentrated solution;

[0009] S2. Ethyl acetate is added to the concentrated solution obtained in step S1, and extracted 2-4 times, and the organic layer is combined and concentrated to obtain a concentrated organic layer;

[0010] S3. An equal volume of 20wt%-30wt% cyclodextrin aqueous solution is added to the concentrated organic layer obtained in step S2, and reverse phase extraction is carried out by inclusion, and after standing and layering, the water layer is collected, and the extraction is repeated once, and the water layers are combined to obtain a cyclodextrin inclusion water layer;

[0011] S4. Sodium chloride and ethyl acetate are added to the cyclodextrin inclusion water layer obtained in step S3, and stirred and extracted at 50-70℃ to dissociate, and the organic layer is collected and concentrated to obtain glycyrrhizic chalcone A crude crystals;

[0012] S5. The glycyrrhizic chalcone A crude crystals obtained in step S4 are dissolved with 92wt%-97wt% ethanol aqueous solution, and decolorized with activated carbon, filtered, and water is slowly added to the decolorized filtrate to adjust the concentration of the ethanol aqueous solution to 60wt%-70wt%, and then cooled to crystallize, filtered, washed, and dried to obtain glycyrrhizic chalcone A.

[0013] The reaction mechanism and action of the present application are as follows:

[0014] In one aspect, cyclodextrin has a unique cylindrical cavity structure, which is hydrophobic inside and hydrophilic outside. Glycyrrhetin A is not soluble in water, but easily soluble in methanol, ethanol, ethyl acetate, acetone, dichloromethane ether and other solvents, and its molecular size can be embedded in the cavity of cyclodextrin to form a stable inclusion complex. This inclusion can increase the solubility of glycyrrhetin A in aqueous solution, making it easier to be extracted and separated. Based on this principle, the present application uses cyclodextrin inclusion-dissociation technology to replace the traditional column chromatography, making the production of glycyrrhetin A more economical and environmentally friendly.

[0015] On the other hand, the water solubility of β-cyclodextrin is relatively low and the inclusion ability is general. The present application improves the water solubility and inclusion ability by chemical modification. The applicant modifies β-cyclodextrin with aspartic acid to obtain a cyclodextrin aqueous solution, and finds that grafting aspartic acid on β-cyclodextrin is beneficial to improving the inclusion ability of glycyrrhetin A, thereby improving the purity of glycyrrhetin A.

[0016] In some embodiments, the mass ratio of the licorice and the aqueous ethanol solution in step S1 is 1:(8-15).

[0017] Preferably, the mass ratio of the licorice and the aqueous ethanol solution in step S1 is 1:(10-12).

[0018] In some embodiments, the volume of the concentrated solution in step S1 is 1 / 15-1 / 5 of the volume of the extraction liquid, and the volume of the concentrated organic layer in step S2 is 1 / 4-1 / 2 of the volume of the organic layer.

[0019] In some embodiments, the volume ratio of the concentrated solution and ethyl acetate in step S2 is 1:(1-2).

[0020] In some embodiments, the inclusion reverse phase extraction conditions in step S3 are normal temperature extraction, stirring speed 400-1000 r / min, and stirring time 2-4 h.

[0021] In some embodiments, the preparation method of the cyclodextrin aqueous solution in step S3 comprises the following steps:

[0022] Q1. Mix β-cyclodextrin and aqueous sodium hydroxide solution, ultrasonic treatment, dropwise add p-toluenesulfonyl chloride acetonitrile solution, react for 1.5-2.5 h, filter, adjust the solution to pH acidic, store at 0-5℃, filter, recrystallize, dry to obtain the product;

[0023] Q2. Mix the product obtained in step Q1, aspartic acid, water, and triethylamine, heat to 75-90℃ under inert gas protection, stir and reflux for 12-20 h, filter, concentrate under reduced pressure, then pour into anhydrous ethanol, filter, purify, dry to obtain a cyclodextrin derivative;

[0024] Q3. Mixing the cyclodextrin derivative obtained in step Q2 with water to obtain a 20wt%-30wt% cyclodextrin aqueous solution.

[0025] In some embodiments, the molar ratio of the product and aspartic acid in step Q2 is 1:(2-3).

[0026] In some embodiments, the volume ratio of the cyclodextrin inclusion water layer and ethyl acetate in step S4 is 1:1.

[0027] Preferably, the volume ratio of the cyclodextrin inclusion water layer and ethyl acetate in step S4 is 1:1.

[0028] In some embodiments, the stirring speed for the stirring and extraction in step S4 is 200-800 r / min, and the stirring time is 1-3 h.

[0029] In some embodiments, after the cyclodextrin inclusion water layer is dissociated and extracted in step S4, the sodium chloride can be removed through a nanofiltration membrane, and the process can be repeated for step S3.

[0030] In some embodiments, the mass ratio of the glycyrrhizin chalcone A crude crystal, the ethanol aqueous solution, and the activated carbon in step S5 is 1:(3-5):(0.05-0.1).

[0031] In some embodiments, the purity of the glycyrrhizin chalcone A obtained by the purification method is ≥95%.

[0032] Compared with the prior art, the present application has the following advantages:

[0033] 1. In the purification process of glycyrrhizin chalcone A, the present application uses ethanol, ethyl acetate, and water as solvents, discarding the use of toxic and banned reagents, simplifying the process flow, effectively reducing production costs, and being suitable for industrial production, which is conducive to the application of glycyrrhizin chalcone A in the fields of cosmetics and medicine.

[0034] 2. The present application does not use traditional column chromatography throughout the process, simplifying the production process, reducing solvent consumption, and making the production of glycyrrhizin chalcone A more economical and environmentally friendly.

[0035] 3. The present application uses cyclodextrin inclusion-dissociation technology to replace traditional column chromatography, making the production of glycyrrhizin chalcone A more economical and environmentally friendly; at the same time, the cyclodextrin aqueous solution of the present application is prepared from aspartic acid modified β-cyclodextrin, which is conducive to improving the inclusion ability of glycyrrhizin chalcone A and thus improving the purity of glycyrrhizin chalcone A. DETAILED DESCRIPTION

[0036] The application will be described in detail below with reference to specific embodiments. It should be noted that the following examples are used to illustrate the application and are not intended to limit the application. Other combinations and various modifications within the concept of the application can be made without departing from the spirit or scope of the application.

[0037] Glycyrrhizin chalcone A was extracted according to the ratio of each raw material and the process as specified in the following example.

[0038] For the convenience of those skilled in the art to implement the application, the raw materials of the application are not specially described and can be purchased from the market.

[0039] Preparation Example 1

[0040] The preparation method of the cyclodextrin aqueous solution A comprises the following steps:

[0041] Q1. 15 g of β-cyclodextrin, 250 mL of 0.3 mol / L sodium hydroxide aqueous solution were mixed, ultrasonic treatment was performed for 20 min, 5 mL of 0.25 g / mL p-toluenesulfonyl chloride acetonitrile solution was slowly added under the control of reaction temperature at 10°C, the dropping time was 5 min, the reaction was performed for 2 h, filtration was performed, the solution was adjusted to pH 3, and was stored at 3°C for 16 h, after a large amount of white precipitate was precipitated, filtration was performed, recrystallization was performed, and vacuum drying was performed at 60°C for 5 h to obtain the product;

[0042] Q2. 1 mmol of the product obtained in step Q1, 2.5 mmol of aspartic acid, 30 mL of water, and 20 mL of triethylamine were mixed, heating was performed to 80°C under nitrogen protection, stirring reflux reaction was performed for 16 h, filtration was performed, vacuum concentration was performed, and then the product was poured into 250 mL of vigorously stirred anhydrous ethanol, a large amount of solid was precipitated, filtration was performed, the solid was dissolved in 150 mL of 50°C water, hot filtration was performed, cooling was performed to room temperature to crystallize, filtration was performed, the crystals were collected, washed with water for 2 times, and vacuum drying was performed at 60°C to constant weight to obtain the cyclodextrin derivative;

[0043] Q3. The cyclodextrin derivative obtained in step Q2 and water were mixed according to the required concentration to obtain the cyclodextrin aqueous solution A.

[0044] Preparation Example 2

[0045] The preparation method of the cyclodextrin aqueous solution B is the same as that in Preparation Example 1, except that the addition amount of aspartic acid in step Q2 is 1.8 mmol.

[0046] Preparation Example 3

[0047] The preparation method of the cyclodextrin aqueous solution C comprises the following steps:

[0048] The β-cyclodextrin and water were mixed according to the requirements to obtain the cyclodextrin aqueous solution C.

[0049] Example 1

[0050] A high-efficiency purification method of glycyrrhiza chalcone A based on cyclodextrin inclusion-dissociation, comprising the following steps:

[0051] S1. After drying licorice at 60℃ to constant weight, crushing, taking 1 kg of crushed licorice, adding to 11 kg of 80wt% ethanol aqueous solution, heating to 75℃ reflux extraction for 2h, extracting 2 times, combining the extract and concentrating to 1 / 10 of the original volume to obtain a concentrated solution;

[0052] S2. Adding 1.5 times the volume of ethyl acetate to the concentrated solution obtained in step S1, extracting 3 times, combining the organic layers and concentrating to 1 / 3 of the original volume to obtain a concentrated organic layer;

[0053] S3. Adding an equal volume of 25wt% cyclodextrin aqueous solution A to the concentrated organic layer obtained in step S2, stirring at room temperature, inclusion extraction at 600r / min for 3h, collecting the water layer after standing and separating, repeating the extraction once, combining the water layers to obtain a cyclodextrin inclusion water layer;

[0054] S4. Adding 10% (m / v) of sodium chloride to the cyclodextrin inclusion water layer obtained in step S3, and adding an equal volume of ethyl acetate to the cyclodextrin inclusion water layer, stirring and extracting at 60℃, stirring speed 500r / min, stirring time 2h, collecting the organic layer and concentrating to 1 / 5 of the original volume, centrifuging to remove the gummy mother liquor to obtain glycyrrhiza chalcone A crude crystals;

[0055] S5. Dissolving the glycyrrhiza chalcone A crude crystals obtained in step S4 with 4 times the mass of 95wt% ethanol aqueous solution relative to the crude crystals, heating to 70℃, and decolorizing with 7.5wt% activated carbon relative to the crude crystals, filtering, adding water to the decolorized filtrate, adjusting the ethanol aqueous solution concentration to 65wt%, cooling to room temperature to precipitate crystals, suction filtration, washing twice with 65wt% ethanol aqueous solution, drying at 80℃ to constant weight to obtain glycyrrhiza chalcone A.

[0056] Example 2

[0057] A high-efficiency purification method of glycyrrhiza chalcone A based on cyclodextrin inclusion-dissociation, comprising the following steps:

[0058] S1. After drying licorice at 60℃ to constant weight, crushing, taking 1 kg of crushed licorice, adding to 11 kg of 80wt% ethanol aqueous solution, heating to 75℃ reflux extraction for 2h, extracting 2 times, combining the extract and concentrating to 1 / 10 of the original volume to obtain a concentrated solution;

[0059] S2. To the concentrated solution obtained in step S1, add 1.5 times the volume of the concentrated solution of ethyl acetate, extract 2 times, combine the organic layers and concentrate to 1 / 2 of the original volume to obtain a concentrated organic layer;

[0060] S3. To the concentrated organic layer obtained in step S2, add an equal volume of 20wt% aqueous cyclodextrin solution A, stir at room temperature, and perform inclusion extraction at 1000r / min for 2h. After standing and layering, collect the water layer, repeat the extraction once, and combine the water layers to obtain a cyclodextrin inclusion water layer;

[0061] S4. To the cyclodextrin inclusion water layer obtained in step S3, add 5% (m / v) of sodium chloride relative to the cyclodextrin inclusion water layer, and add an equal volume of ethyl acetate relative to the cyclodextrin inclusion water layer. Stir and extract at 70°C, with a stirring speed of 800r / min and a stirring time of 1h. Collect the organic layer and concentrate to 1 / 5 of the original volume. Centrifuge to remove the gummy mother liquor to obtain crude glycyrrhetin A crystals;

[0062] S5. Dissolve the crude glycyrrhetin A crystals obtained in step S4 in 3 times the mass of 97wt% aqueous ethanol solution relative to the crude crystals, at a temperature of 70°C. Decolorize with 5wt% activated carbon relative to the crude crystals, filter, add water to the decolorized filtrate, adjust the aqueous ethanol concentration to 70wt%, and precipitate the crystals at room temperature. Vacuum filter, wash twice with 70wt% aqueous ethanol, and dry at 80°C to constant weight to obtain glycyrrhetin A.

[0063] Example 3

[0064] A high-efficiency purification method for glycyrrhetin A based on cyclodextrin inclusion-dissociation, comprising the following steps:

[0065] S1. After drying the licorice at 60°C to constant weight, crush it. Take 1kg of crushed licorice and add it to 15kg of 60wt% aqueous ethanol solution. Heat to 75°C and reflux extract for 2h. Extract 2 times, combine the extract and concentrate to 1 / 15 of the original volume to obtain a concentrated solution;

[0066] S2. To the concentrated solution obtained in step S1, add 1.5 times the volume of the concentrated solution of ethyl acetate, extract 4 times, combine the organic layers and concentrate to 1 / 4 of the original volume to obtain a concentrated organic layer;

[0067] S3. To the concentrated organic layer obtained in step S2, add an equal volume of 30wt% aqueous cyclodextrin solution A, stir at room temperature, and perform inclusion extraction at 400r / min for 4h. After standing and layering, collect the water layer, repeat the extraction once, and combine the water layers to obtain a cyclodextrin inclusion water layer;

[0068] S4. To the cyclodextrin inclusion water layer obtained in step S3, add 15% (m / v) of sodium chloride relative to the cyclodextrin inclusion water layer, and add an equal volume of ethyl acetate to the cyclodextrin inclusion water layer, stir and dissolve at 50°C, and extract, the stirring speed is 200 r / min, the stirring time is 3 h, collect the organic layer and concentrate to 1 / 5 of the original volume, centrifuge to remove the gummy mother liquor, to obtain glycyrrhetinyl chalcone A crude crystals;

[0069] S5. The glycyrrhetinyl chalcone A crude crystals obtained in step S4 are dissolved by heating with 5 times the mass of 92 wt% ethanol aqueous solution relative to the crude crystals, the temperature is 70°C, and decolorized with 10 wt% activated carbon relative to the crude crystals, filtered, add water to the decolorized filtrate, adjust the ethanol aqueous solution concentration to 60 wt%, precipitate crystals at room temperature, suction filter, wash twice with 60 wt% ethanol aqueous solution, and dry at 80°C to constant weight to obtain glycyrrhetinyl chalcone A.

[0070] Example 4

[0071] A glycyrrhetinyl chalcone A efficient purification method based on cyclodextrin inclusion-dissociation, the specific implementation is the same as example 1, the difference is that the amount of 80 wt% ethanol aqueous solution added in step S1 is 7 kg.

[0072] Example 5

[0073] A glycyrrhetinyl chalcone A efficient purification method based on cyclodextrin inclusion-dissociation, the specific implementation is the same as example 1, the difference is that in step S3, an equal amount of cyclodextrin aqueous solution B is used instead of cyclodextrin aqueous solution A.

[0074] Example 6

[0075] A glycyrrhetinyl chalcone A efficient purification method based on cyclodextrin inclusion-dissociation, the specific implementation is the same as example 1, the difference is that in step S3, an equal amount of cyclodextrin aqueous solution C is used instead of cyclodextrin aqueous solution A.

[0076] Example 7

[0077] A glycyrrhetinyl chalcone A efficient purification method based on cyclodextrin inclusion-dissociation, the specific implementation is the same as example 1, the difference is that in step S4, 4% (m / v) of sodium chloride relative to the cyclodextrin inclusion water layer is added.

[0078] Example 8

[0079] A glycyrrhetinyl chalcone A efficient purification method based on cyclodextrin inclusion-dissociation, the specific implementation is the same as example 1, the difference is that in step S5, 2.5 times the mass of 95 wt% ethanol aqueous solution relative to the crude crystals is used to heat and dissolve.

[0080] Example 9

[0081] A high-efficiency purification method of glycyrrhizin chalcone A based on cyclodextrin inclusion-dissociation, the specific implementation is the same as example 1, the difference is that the relative crude crystal 4wt% activated carbon decolorization in step S5.

[0082] Effect evaluation:

[0083] The glycyrrhizin chalcone A extracted in the above examples 1-9 was tested and analyzed, and the specific results are shown in Table 1.

[0084] Performance test:

[0085] The purity of glycyrrhizin chalcone A in glycyrrhizin extract was detected by HPLC, wherein the chromatographic column was C18 column, the mobile phase was acetonitrile:0.1% phosphoric acid aqueous solution=47:53(v / v), the detection wavelength was 372nm, and the flow rate was 1mL / min.

[0086] Table 1

[0087] Serial number Purity / % Example 1 98.3 Example 2 97.6 Example 3 98.2 Example 4 92.5 Example 5 94.7 Example 6 93.3 Example 7 94.2 Example 8 93.6 Example 9 94.4

[0088] From the results in Table 1, it can be seen that the purity of glycyrrhizin chalcone A obtained in examples 1-3 is high, and has good application value.

[0089] Compared with example 1, the mass ratio of licorice and ethanol aqueous solution in step S1 of example 4 is changed, which may make the extraction selectivity worse, which is not conducive to the subsequent purification of glycyrrhizin chalcone A.

[0090] Compared with example 1, the molar ratio of product and aspartic acid in the preparation process of cyclodextrin aqueous solution is changed in example 5, and the cyclodextrin aqueous solution modified by aspartic acid is not used in example 6, which will reduce the inclusion capacity of glycyrrhizin chalcone A, and then affect the purity of glycyrrhizin chalcone A.

[0091] Compared with example 1, the amount of sodium chloride added in step S4 of example 7 is changed, the separation effect of glycyrrhizin chalcone A from cyclodextrin inclusion water layer is poor, which is not conducive to the separation and purification of glycyrrhizin chalcone A.

[0092] Compared with example 1, the mass ratio of glycyrrhizin chalcone A crude crystal, ethanol aqueous solution and activated carbon is changed in examples 8-9, which will make the glycyrrhizin chalcone A crude crystal not fully dissolved in example 8, and the decolorization effect will be reduced in example 9, which will reduce the removal of impurities in glycyrrhizin chalcone A crude crystal, and then reduce the purity of glycyrrhizin chalcone A.

[0093] The above merely describes preferred embodiments of the present application, and is not intended to limit the present application in any form. Although the present application is disclosed as above with preferred embodiments, it is not intended to limit the present application. Any person skilled in the art, without departing from the scope of the technical scheme of the present application, can make some changes or modifications to the disclosed technical content, which are equivalent to equivalent embodiments. Any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present application, without departing from the technical scheme content of the present application, are still within the scope of the technical scheme.

Claims

1. A method for high-efficiency purification of glycyrrhiza chalcone A based on cyclodextrin inclusion-dissociation, characterized in that, The method comprises the following steps: S1. After drying and crushing licorice, extract it with 60wt%-95wt% ethanol aqueous solution for 2-3 times, combine the extract and concentrate it to obtain a concentrated solution; S2. Add ethyl acetate to the concentrated solution obtained in step S1, extract it for 2-4 times, combine the organic layers and concentrate it to obtain a concentrated organic layer; S3. Add an equal volume of 20wt%-30wt% cyclodextrin aqueous solution to the concentrated organic layer obtained in step S2, perform inclusion reverse phase extraction, collect the water layer after static layer separation, repeat the extraction for 1 time, combine the water layers to obtain a cyclodextrin inclusion water layer; S4. Add sodium chloride and ethyl acetate to the cyclodextrin inclusion water layer obtained in step S3, stir and dissociate at 50-70°C, collect the organic layer and concentrate it to obtain crude licorice chalcone A crystals; S5. Dissolve the crude licorice chalcone A crystals obtained in step S4 in 92wt%-97wt% ethanol aqueous solution, decolorize it with activated carbon, filter it, slowly add water to the decolorized filtrate, adjust the concentration of the ethanol aqueous solution to 60wt%-70wt%, cool it to crystallize, suction filter, wash and dry it to obtain licorice chalcone A.

2. The method according to claim 1, wherein the method is a high-performance purification method of glycyrrhiza chalcone A based on cyclodextrin inclusion-dissociation. The mass ratio of the licorice to the ethanol aqueous solution in step S1 is 1:(8-15).

3. The method according to claim 1, wherein the method is characterized by, The volume of the concentrated solution in step S1 is 1 / 15-1 / 5 of the volume of the extract, and the volume of the concentrated organic layer in step S2 is 1 / 4-1 / 2 of the volume of the organic layer.

4. The method according to claim 1, wherein the method is characterized by, The inclusion reverse phase extraction conditions in step S3 are normal temperature extraction, a stirring speed of 400-1000r / min and a stirring time of 2-4h.

5. The method according to claim 1, wherein the method is characterized by, The preparation method of the cyclodextrin aqueous solution in step S3 comprises the following steps: Q1. Mix β-cyclodextrin and sodium hydroxide aqueous solution, ultrasonically treat it, dropwise add p-toluenesulfonyl chloride acetonitrile solution, react for 1.5-2.5h, filter it, adjust the solution to be acidic, store it at 0-5°C, filter it, recrystallize it and dry it to obtain a product; Q2. Mix the product obtained in step Q1, aspartic acid, water and triethylamine, heat it to 75-90°C under inert gas protection, stir and reflux it to react for 12-20h, filter it, concentrate it under reduced pressure, pour it into anhydrous ethanol, filter it, purify it and dry it to obtain a cyclodextrin derivative; Q3. Mix the cyclodextrin derivative obtained in step Q2 and water to obtain a 20wt%-30wt% cyclodextrin aqueous solution.

6. The method according to claim 5, wherein the method is a high-performance purification method of glycyrrhiza chalcone A based on cyclodextrin inclusion-dissociation. The molar ratio of the product to aspartic acid in step Q2 is 1:(2-3).

7. The method according to claim 1, wherein the method is based on the inclusion-dissociation of cyclodextrin for the purification of glycyrrhiza chalcone A. The ratio of the amount of the cyclodextrin inclusion water layer to sodium chloride in step S4 is 1mL:(0.05-0.15)g.

8. The method according to claim 1, wherein the method is a high-performance purification method of glycyrrhiza chalcone A based on cyclodextrin inclusion-dissociation. The stirring and dissociation conditions in step S4 are a stirring speed of 200-800r / min and a stirring time of 1-3h.

9. The method according to claim 1, wherein the method is a high-performance purification method of glycyrrhiza chalcone A based on cyclodextrin inclusion-dissociation. The mass ratio of the crude licorice chalcone A, the ethanol aqueous solution and the activated carbon in step S5 is 1:(3-5):(0.05-0.1).

10. The method according to any one of claims 1-9, wherein the method is a high- efficiency purification method of glycyrrhiza chalcone A based on cyclodextrin inclusion-dissociation. The purity of the licorice chalcone A obtained by the purification method is ≥95%.