Microbial composition and rice seedling growth-promoting microbial agent containing microbial composition
By preparing rice seedling growth-promoting bacteria through microbial compositions, the problems of low utilization rate of chemical fertilizers and frequent rice diseases have been solved, thereby improving soil fertility, controlling diseases, and promoting rice growth and yield.
Patent Information
- Application Number
- CN202511090368.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-05
- Publication Date
- 2025-11-07
AI Technical Summary
The low utilization rate of existing chemical fertilizers leads to soil pollution and ecological damage, while rice growth is poor, yield is difficult to increase, and diseases are frequent.
A microbial composition consisting of Pseudomonas fluorescens, Bacillus subtilis, Trichoderma harzianum, Bacillus amyloliquefaciens, Bacillus megaterium, Streptomyces, nitrogen-fixing bacteria, and potassium-solubilizing bacteria was used to prepare a rice seedling growth-promoting agent through fermentation culture, which promotes soil fertility improvement and disease control.
It improves soil fertility, promotes nutrient absorption by rice seedlings, enhances stress resistance and growth, inhibits various soil-borne diseases, increases rice yield, and reduces environmental pollution caused by the use of chemical fertilizers.
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Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of microbial inoculants, and particularly relates to a microbial composition and a rice seedling growth-promoting microbial inoculant containing the same. BACKGROUND
[0002] Rice is one of the important food crops, and with the continuous growth of food demand, the demand for fertilizer is also increasing. At present, chemical fertilizer still occupies a dominant position in agricultural production, but the utilization rate of chemical fertilizer is low, and a large amount of chemical fertilizer is applied to the soil, which not only causes soil pollution, but also destroys the ecological environment, leading to poor growth of rice, difficult improvement of yield and decline of rice quality, especially frequent occurrence of rice diseases. Although there are various types of fertilizers on the market at present, including biological fertilizers, organic compound fertilizers and the like, there are still certain limitations in the play of fertilizer, improvement of soil and prevention of rice diseases. SUMMARY
[0003] The present application aims to provide a microbial composition and a rice seedling growth-promoting microbial inoculant containing the same, which are synergistically matched and interacted with each other, can effectively decompose organic matter in the soil, improve soil fertility, promote the absorption of nutrient substances by rice seedlings, and have inhibitory effect on various diseases, thereby comprehensively ensuring the growth and development of rice seedlings and improving the yield of rice.
[0004] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions:
[0005] The present application provides a microbial composition, which comprises the following components in parts by weight: 6-15 parts of Pseudomonas fluorescens, 5-10 parts of Bacillus subtilis, 5-10 parts of Trichoderma harzianum, 15-20 parts of Bacillus amyloliquefaciens, 8-12 parts of Bacillus megaterium, 3-6 parts of Streptomyces, 5-10 parts of nitrogen-fixing bacteria and 1-3 parts of potassium-dissolving bacteria.
[0006] Preferably, the potassium-dissolving bacteria comprise Bacillus mucilaginosus or Bacillus circulans.
[0007] Preferably, the nitrogen-fixing bacteria comprise any two or more of Bradyrhizobium tropic, Sinorhizobium meliloti, Rhizobium leguminosarum and Azotobacter chroococcum.
[0008] Preferably, when the nitrogen-fixing bacteria are Sinorhizobium meliloti and Azotobacter chroococcum, the mass ratio of the Rhizobium leguminosarum and the Azotobacter chroococcum is (1-1.5):1.
[0009] The present application provides an application of the microbial composition in the preparation of a growth-promoting microbial inoculant for promoting the growth and development of rice seedlings.
[0010] The application provides application of the microbial composition in preparation of a growth promoting fertilizer for promoting growth and development of rice seedlings.
[0011] The application also provides a rice seedling growth promoting microbial agent containing the microbial composition.
[0012] Preferably, the effective viable cell count in the rice seedling growth promoting microbial agent is greater than or equal to 1.2*10 10 cfu / mL.
[0013] The application also provides a preparation method of the rice seedling growth promoting microbial agent, comprising the following steps:
[0014] The Pseudomonas fluorescens, Bacillus subtilis, Trichoderma harzianum, Bacillus amyloliquefaciens, Bacillus megaterium, Streptomyces, nitrogen-fixing bacteria and potassium-dissolving bacteria are inoculated and cultured to obtain bacterial suspensions, and then the bacterial suspensions are inoculated into a fermentation medium to perform fermentation culture, so that the rice seedling growth promoting microbial agent is obtained.
[0015] Preferably, the pH of the fermentation medium is 6.5-7.3.
[0016] Preferably, the fermentation culture is performed for 4-6 days, and the fermentation culture is performed at a temperature of 28-33 DEG C.
[0017] By using the above technical scheme, the application has the following beneficial effects:
[0018] 1. The nitrogen-fixing bacteria and potassium-dissolving bacteria are used in combination in the technical scheme of the application, which can not only convert potassium minerals in the soil that are difficult for plants to utilize into potassium that can be utilized by plants, but also convert gaseous nitrogen into ammonia nitrogen and nitrate nitrogen that can be absorbed by plants, so that the soil fertility is better improved, the absorption and utilization of potassium and nitrogen by plants are promoted, and thus the stress resistance and yield of plants are enhanced; meanwhile, active substances such as gibberellins, cytokinins and indole acetic acid are produced in the life activities, which further stimulate the growth and development of rice.
[0019] 2. The Pseudomonas fluorescens, Bacillus subtilis, Trichoderma harzianum, Bacillus amyloliquefaciens and other bacteria are used in combination in the application, and the bacteria interact with and affect each other, so that the organic matter in the soil can be effectively decomposed, the soil structure is improved, the soil fertility is improved, the absorption of nutrient substances by rice seedlings is promoted, and the growth and development are enhanced; the bacteria also have inhibitory effects on various soil-borne diseases, and can effectively prevent the occurrence of root rot, sheath blight, fusarium wilt, rice blast and other soil-borne diseases, so that the growth and development of rice seedlings are comprehensively ensured, and the yield of rice is improved.
[0020] 3. The microbial composition in the technical scheme of the application has low requirements for the culture environment of the bacteria, and the manufacturing process is simple, so that the manufacturing cost can be effectively reduced.
[0021] 4. The use of the microbial composition in the rice planting according to the technical scheme of the present application can also effectively reduce the use of chemical fertilizers and the like, and reduce the influence on the soil structure and the pollution to the ecological environment. DETAILED DESCRIPTION
[0022] The present application provides a microbial composition, comprising the following components: Pseudomonas fluorescens, Bacillus subtilis, Trichoderma harzianum, Bacillus amyloliquefaciens, Bacillus megaterium, Streptomyces, nitrogen-fixing bacteria and potassium-dissolving bacteria.
[0023] In the present application, the weight fraction of Pseudomonas fluorescens is preferably 6-15 parts, further preferably 9-12 parts, and more preferably 10 parts; the weight fraction of Bacillus subtilis is preferably 5-10 parts, further preferably 6-9 parts, and more preferably 8 parts; the weight fraction of Trichoderma harzianum is preferably 5-10 parts, further preferably 6-9 parts, and more preferably 7 parts; the weight fraction of Bacillus amyloliquefaciens is preferably 15-20 parts, further preferably 16-19 parts, and more preferably 18 parts; the weight fraction of Bacillus megaterium is preferably 8-12 parts, further preferably 9-11 parts, and more preferably 10 parts; the weight fraction of Streptomyces is preferably 3-6 parts, further preferably 3.5-5.5 parts, and more preferably 4.5 parts; the weight fraction of nitrogen-fixing bacteria is preferably 5-10 parts, further preferably 7-9 parts, and more preferably 8 parts; and the weight fraction of potassium-dissolving bacteria is preferably 1-3 parts, further preferably 1.4-2.5 parts, and more preferably 2.2 parts.
[0024] In the present application, the potassium-dissolving bacteria preferably include Bacillus mucilaginosus or Bacillus circulans, and further preferably Bacillus mucilaginosus.
[0025] In the present application, the nitrogen-fixing bacteria preferably include any two or more of Bradyrhizobium tropic, Sinorhizobium meliloti, Rhizobium leguminosarum and Azotobacter chroococcum.
[0026] In the present application, when the nitrogen-fixing bacteria are Rhizobium leguminosarum and Azotobacter chroococcum, the mass ratio of Sinorhizobium meliloti and Azotobacter chroococcum is preferably (1-1.5) : 1, further preferably (1.1-1.4) : 1, and more preferably 1.2:1.
[0027] The present application also provides the use of the microbial composition in the preparation of a growth-promoting microbial agent for promoting the growth and development of rice seedlings.
[0028] The present application also provides the use of the microbial composition in the preparation of a growth-promoting fertilizer for promoting the growth and development of rice seedlings.
[0029] The present application also provides a rice seedling growth promoting microbial agent comprising the microbial composition.
[0030] In the present application, the effective viable cell number in the rice seedling growth promoting microbial agent is preferably ≥1.2×10 10 cfu / mL.
[0031] The present application also provides a preparation method of the rice seedling growth promoting microbial agent, comprising the following steps: inoculating Pseudomonas fluorescens, Bacillus subtilis, Trichoderma harzianum, Bacillus amyloliquefaciens, Bacillus megaterium, Streptomyces, Azotobacter and Potassium solubilizing bacteria into culture medium respectively to obtain bacterial suspension, and then inoculating each bacterial suspension into fermentation medium to perform fermentation culture, thereby obtaining the rice seedling growth promoting microbial agent.
[0032] In the present application, Pseudomonas fluorescens, Bacillus subtilis, Trichoderma harzianum, Bacillus amyloliquefaciens, Bacillus megaterium, Streptomyces, Azotobacter and Potassium solubilizing bacteria are inoculated into culture medium respectively to obtain bacterial suspension, and the culture medium used is preferably LB culture medium, PDA culture medium or YEME culture medium. In the present application, the culture medium inoculated with Pseudomonas fluorescens, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus megaterium, Rhizobium leguminosarum and Potassium solubilizing bacteria is preferably LB culture medium, the culture medium inoculated with Azotobacter chroococcum and Trichoderma harzianum is preferably PDA culture medium, and the culture medium inoculated with Streptomyces is preferably YEME culture medium. The culture time in the present application is preferably 18-36h, further preferably 20-32h, and more preferably 28h. The effective viable cell number in the bacterial suspension of Bacillus subtilis obtained in the present application is preferably ≥5×10 9 cfu / mL; the effective viable cell number in the bacterial suspension of Pseudomonas fluorescens is preferably ≥4.5×10 9 cfu / mL; the effective viable cell number in the bacterial suspension of Trichoderma harzianum is preferably ≥1.2×10 9 cfu / mL; the effective viable cell number in the bacterial suspension of Bacillus amyloliquefaciens is preferably ≥1×10 10 cfu / mL; the effective viable cell number in the bacterial suspension of Bacillus megaterium is preferably ≥7.5×10 9 cfu / mL; the effective viable cell number in the bacterial suspension of Streptomyces is preferably ≥1×10 8 cfu / mL; the effective viable cell number in the bacterial suspension of Rhizobium leguminosarum is preferably ≥4.5×10 9 cfu / mL; the effective viable cell number in the bacterial suspension of Azotobacter chroococcum is preferably ≥5×10 8 cfu / mL; and the effective viable cell number in the bacterial suspension of Potassium solubilizing bacteria is preferably ≥9×10 9 cfu / mL.
[0033] In the present application, the obtained bacterial suspension is inoculated into a fermentation medium for fermentation culture, and then a rice seedling growth promoting bacterial agent is obtained. The fermentation medium of the present application uses water as a solvent, and preferably comprises the following components in the mass percentage: glucose 10-15 g / L, beef extract 2-4 g / L, calcium carbonate 0.8-1.2 g / L, soybean powder 6-9 g / L, and NaCl 1-3 g / L; further preferably, it comprises glucose 11-13 g / L, beef extract 2.5-3.5 g / L, calcium carbonate 0.9-1.1 g / L, soybean powder 7-8.5 g / L, and NaCl 1.5-2.5 g / L; and more preferably, it comprises glucose 12 g / L, beef extract 3 g / L, calcium carbonate 1 g / L, soybean powder 8 g / L, and NaCl 2 g / L.
[0034] In the present application, the pH of the fermentation medium is preferably 6.5-7.3.
[0035] In the present application, the fermentation culture time is preferably 4-6 d, and further preferably 5 d; and the fermentation culture temperature is preferably 28-33℃, further preferably 29-32℃, and more preferably 30℃.
[0036] The technical solutions provided by the present application are described in detail below in conjunction with the examples, but they should not be understood as limiting the scope of protection of the present application.
[0037] The Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus megaterium, Streptomyces, Rhizobium leguminosarum, and Kinetica potens in the present application are purchased from the China Agricultural Microbial Culture Collection Center; the Trichoderma harzianum is purchased from Weifang Ruichen Biotechnology Co., Ltd.; and the Pseudomonas fluorescens, Azotobacter chroococcum, and Rhizobium leguminosarum are purchased from Ningbo Mingzhou Biotechnology Co., Ltd.
[0038] Example 1
[0039] A microbial composition, comprising: 10 parts of Pseudomonas fluorescens, 8 parts of Bacillus subtilis, 7 parts of Trichoderma harzianum, 18 parts of Bacillus amyloliquefaciens, 10 parts of Bacillus megaterium, 4.5 parts of Streptomyces, 8 parts of nitrogen-fixing bacteria, and 2.2 parts of Bacillus mucilaginosus.
[0040] The nitrogen-fixing bacteria are Rhizobium leguminosarum and Azotobacter chroococcum in a mass ratio of 1.2:1.
[0041] Example 2
[0042] A microbial composition, comprising: 9 parts of Pseudomonas fluorescens, 8 parts of Bacillus subtilis, 7 parts of Trichoderma harzianum, 18 parts of Bacillus amyloliquefaciens, 10 parts of Bacillus megaterium, 4.5 parts of Streptomyces, 5 parts of nitrogen-fixing bacteria, and 2.2 parts of Bacillus circulans.
[0043] The nitrogen-fixing bacteria are pea root nodule bacteria and azotobacter chroococcum at a mass ratio of 1.5:1.
[0044] Example 3
[0045] A microbial composition, components of which are as follows: 8 parts of Pseudomonas fluorescens, 5 parts of Bacillus subtilis, 10 parts of Trichoderma harzianum, 15 parts of Bacillus amyloliquefaciens, 12 parts of Bacillus megaterium, 6 parts of Streptomyces, 10 parts of nitrogen-fixing bacteria, and 3 parts of Bacillus mucilaginosus.
[0046] The nitrogen-fixing bacteria are pea root nodule bacteria and azotobacter chroococcum at a mass ratio of 1:1.
[0047] Example 4
[0048] A microbial composition, components of which are as follows: 6 parts of Pseudomonas fluorescens, 10 parts of Bacillus subtilis, 5 parts of Trichoderma harzianum, 20 parts of Bacillus amyloliquefaciens, 8 parts of Bacillus megaterium, 3 parts of Streptomyces, 6 parts of nitrogen-fixing bacteria, and 1 part of Bacillus mucilaginosus.
[0049] The nitrogen-fixing bacteria are Sinorhizobium meliloti and azotobacter chroococcum at a mass ratio of 1.4:1.
[0050] Example 5
[0051] A rice seedling growth-promoting microbial agent, the preparation method of which is as follows:
[0052] 10 parts of Pseudomonas fluorescens, 8 parts of Bacillus subtilis, 18 parts of Bacillus amyloliquefaciens, 10 parts of Bacillus megaterium, 4.4 parts of pea root nodule bacteria, and 2.2 parts of Bacillus mucilaginosus are inoculated into LB culture medium respectively, 3.6 parts of azotobacter chroococcum and 7 parts of Trichoderma harzianum are inoculated into PDA culture medium, and 4.5 parts of Streptomyces is inoculated into YEME culture medium. After 28 hours of culture respectively, Bacillus subtilis bacterial suspension, Trichoderma harzianum bacterial suspension, Bacillus amyloliquefaciens bacterial suspension, Bacillus megaterium bacterial suspension, Streptomyces bacterial suspension, pea root nodule bacteria bacterial suspension, azotobacter chroococcum bacterial suspension, and Bacillus mucilaginosus bacterial suspension are obtained. Then the bacterial suspensions are inoculated into a fermentation medium with pH of 7.0, and fermentation culture is carried out at 30°C for 5 days, wherein the fermentation medium is composed of components with mass percentage concentrations of glucose 12 g / L, beef extract 3 g / L, calcium carbonate 1 g / L, soybean powder 8 g / L, and NaCl 2 g / L, and water is used as solvent; the effective viable bacterial count in the finally obtained rice seedling growth-promoting microbial agent is ≥1.2×10 10 cfu / mL.
[0053] Example 6
[0054] A rice seedling growth-promoting microbial agent, the preparation method of which is as follows:
[0055] Inoculate 9 parts of Pseudomonas fluorescens, 8 parts of Bacillus subtilis, 18 parts of Bacillus amyloliquefaciens, 10 parts of Bacillus megaterium, 3 parts of Rhizobium leguminosarum and 2.2 parts of Bacillus circulans into LB culture medium, inoculate 2 parts of Azotobacter chroococcum and 7 parts of Trichoderma harzianum into PDA culture medium, and inoculate 4.5 parts of Streptomyces into YEME culture medium. After 28 hours of culture, Bacillus subtilis bacterial suspension, Trichoderma harzianum bacterial suspension, Bacillus amyloliquefaciens bacterial suspension, Bacillus megaterium bacterial suspension, Streptomyces bacterial suspension, Rhizobium leguminosarum bacterial suspension, Azotobacter chroococcum bacterial suspension and Bacillus circulans bacterial suspension are obtained. Then, inoculate each bacterial suspension into fermentation medium with pH of 7.0, and carry out fermentation culture at 30℃ for 5 days. The fermentation medium is composed of glucose 12 g / L, beef extract 3 g / L, calcium carbonate 1 g / L, soybean powder 8 g / L and NaCl 2 g / L with water as solvent. The effective viable bacterial count in the final obtained rice seedling growth promoting bacterial agent is ≥1.2×10 10 cfu / mL.
[0056] Example 7
[0057] A rice seedling growth promoting bacterial agent is prepared by the following method:
[0058] Inoculate 8 parts of Pseudomonas fluorescens, 5 parts of Bacillus subtilis, 15 parts of Bacillus amyloliquefaciens, 12 parts of Bacillus megaterium, 5 parts of Rhizobium leguminosarum and 3 parts of Bacillus circulans into LB culture medium, inoculate 5 parts of Azotobacter chroococcum and 10 parts of Trichoderma harzianum into PDA culture medium, and inoculate 6 parts of Streptomyces into YEME culture medium. After 36 hours of culture, Bacillus subtilis bacterial suspension, Trichoderma harzianum bacterial suspension, Bacillus amyloliquefaciens bacterial suspension, Bacillus megaterium bacterial suspension, Streptomyces bacterial suspension, Rhizobium leguminosarum bacterial suspension, Azotobacter chroococcum bacterial suspension and Bacillus circulans bacterial suspension are obtained. Then, inoculate each bacterial suspension into fermentation medium with pH of 7.1, and carry out fermentation culture at 29℃ for 4 days. The fermentation medium is composed of glucose 12 g / L, beef extract 3 g / L, calcium carbonate 1 g / L, soybean powder 8 g / L and NaCl 2 g / L with water as solvent. The effective viable bacterial count in the final obtained rice seedling growth promoting bacterial agent is ≥1.2×10 10 cfu / mL.
[0059] Comparative Example 1
[0060] A rice seedling growth promoting bacterial agent is prepared by the following method:
[0061] Pseudomonas fluorescens 10 parts, Bacillus subtilis 8 parts, Bacillus amyloliquefaciens 18 parts, Bacillus megaterium 10 parts and Bacillus mycoides 2.2 parts were inoculated into LB culture medium respectively, Trichoderma harzianum 7 parts was inoculated into PDA culture medium, and after 28h of culture respectively, Pseudomonas fluorescens bacterial suspension, Bacillus subtilis bacterial suspension, Trichoderma harzianum bacterial suspension, Bacillus amyloliquefaciens bacterial suspension, Bacillus megaterium bacterial suspension and Bacillus mycoides bacterial suspension were obtained. Then each bacterial suspension was inoculated into a fermentation medium with pH 7.0, and fermented at 30℃ for 5d, wherein the fermentation medium was composed of components with mass percentage concentration of glucose 12g / L, beef extract 3g / L, calcium carbonate 1g / L, soybean powder 8g / L and NaCl 2g / L, and water was used as solvent; the effective viable bacterial number in the final obtained rice seedling growth promoting bacterial agent was ≥1.2×10 10 cfu / mL.
[0062] Comparative Example 2
[0063] A rice seedling growth promoting bacterial agent was prepared by the following method:
[0064] Pseudomonas fluorescens 10 parts, Bacillus subtilis 8 parts, Bacillus amyloliquefaciens 18 parts, Bacillus megaterium 10 parts and 4.4 parts of Rhizobium leguminosarum were inoculated into LB culture medium respectively, 3.6 parts of Azotobacter chroococcum and 7 parts of Trichoderma harzianum were inoculated into PDA culture medium, and 4.5 parts of Streptomyces were inoculated into YEME culture medium, and after 28h of culture respectively, Pseudomonas fluorescens bacterial suspension, Bacillus subtilis bacterial suspension, Trichoderma harzianum bacterial suspension, Bacillus amyloliquefaciens bacterial suspension, Bacillus megaterium bacterial suspension, Streptomyces bacterial suspension, Rhizobium leguminosarum bacterial suspension and Azotobacter chroococcum bacterial suspension were obtained. Then each bacterial suspension was inoculated into a fermentation medium with pH 7.0, and fermented at 30℃ for 5d, wherein the fermentation medium was composed of components with mass percentage concentration of glucose 12g / L, beef extract 3g / L, calcium carbonate 1g / L, soybean powder 8g / L and NaCl 2g / L, and water was used as solvent; the effective viable bacterial number in the final obtained rice seedling growth promoting bacterial agent was ≥1.2×10 10 cfu / mL.
[0065] Comparative Example 3
[0066] A rice seedling growth promoting bacterial agent was prepared by the following method:
[0067] Three samples of *Pseudomonas fluorescens*, two samples of *Bacillus subtilis*, five samples of *Bacillus amyloliquefaciens*, ten samples of *Bacillus megaterium*, one sample of *Rhizobium peatum*, and two and two samples of *Bacillus mucilaginosus* were inoculated into LB medium, respectively. One sample of *Azotobacter chrysophagus* and seven samples of *Trichoderma harzianum* were inoculated into PDA medium, and one sample of *Streptomyces* was inoculated into YEME medium. After culturing for 28 hours, suspensions of *Pseudomonas fluorescens*, *Bacillus subtilis*, *Trichoderma harzianum*, *Bacillus amyloliquefaciens*, *Bacillus megaterium*, *Streptomyces*, *Rhizobium peatum*, *Azotobacter chrysophagus*, and *Bacillus mucilaginosus* were obtained. The bacterial suspensions were then inoculated into a fermentation medium with a pH of 7.0 and fermented at 30°C for 5 days. This fermentation medium, using water as a solvent, consisted of components with a mass percentage concentration of 12 g / L glucose, 3 g / L beef extract, 1 g / L calcium carbonate, 8 g / L soybean flour, and 2 g / L NaCl. The final rice seedling growth-promoting bacterial agent contained ≥1.2 × 10⁻⁶ viable bacteria. 10 cfu / mL.
[0068] Experimental Example 1
[0069] The tested rice variety was Chunguang No. 1. Rice seeds were placed in petri dishes, soaked in water for 24 hours, disinfected with 0.1% HgCl2 solution for 5 minutes, and then rinsed three times with distilled water before further treatment. The rice seeds were randomly divided into seven treatment groups: a control group, Example 5 group, Example 6 group, Example 7 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group. Each treatment was replicated three times. Seedling trays with an area of 60cm × 30cm × 2cm were used, each containing 2000g of fine soil and 200 seedlings per tray. Seven days after sowing, the rice seedling growth-promoting bacteria agents prepared in Examples 5-6 and Comparative Examples 1-3 were diluted with water at a volume ratio of 1:100 and applied to the roots (8g of diluted rice seedling growth-promoting bacteria solution was needed per kg of rice seeds). The control group was applied with the same volume of distilled water. The seedlings were then cultivated at suitable temperature and humidity.
[0070] (I) Seedling Morphology Indicators
[0071] At 30 days of seedling age, 12 seedlings from each treatment were sampled for morphological and physiological index determination, including leaf length (second to last leaf), leaf width (widest part of the second to last leaf), base width (2 cm from the base of the stem), seedling height, root length, number of roots, number of white roots, above-ground fresh weight, and underground fresh weight. The results are shown in Table 1.
[0072] Table 1. Morphological and physiological indicators of seedlings in different treatment groups
[0073]
[0074]
[0075] According to Table 1, it can be seen that the physiological indexes of the rice seedlings treated with the prepared rice seedling growth promoting bacterial agent of the application are relatively best. That is, the prepared rice seedling growth promoting bacterial agent of the example can effectively promote the growth and development of the rice seedlings. (II)
[0077] After spraying the corresponding treatment agent for 5d, the rice seedlings in each group were irrigated with Magnaporthe grisea spore suspension (1x10 7 cfu / mL), and continued to be cultured. Then, the rice seedlings were irrigated once every 7d, and the disease index was investigated when the blank control was fully diseased, and the control effect was calculated.
[0078] Control effect % = (CK lesion length-Pt lesion length) / CK lesion length x 100%
[0079] Wherein, CK lesion length represents the lesion length of the distilled water treatment group; Pt lesion length represents the average length of the lesion of the treated group.
[0080] Table 2: Effect of different treatment groups on rice leaf resistance to rice blast
[0081]
[0082]
[0083] According to Table 2, it can be seen that the rice seedling growth promoting bacterial agent of the application can effectively control the development of rice blast, inhibit the pathogenic bacteria, and better ensure the growth of rice seedlings.
[0084] As can be seen from the above, through the synergistic matching and interaction of each bacterium in the rice seedling growth promoting bacterial agent of the application, the absorption of nutrients by the rice seedlings can be effectively promoted, the growth and development can be enhanced, the occurrence of soil-borne diseases can be inhibited, and the growth and development of rice seedlings can be comprehensively ensured, thereby improving the yield of rice.
[0085] The above only describes the preferred embodiments of the application, and it should be noted that for those skilled in the art, without departing from the principles of the application, a number of improvements and refinements can be made, and these improvements and refinements should also be considered as the protection scope of the application.
Claims
1. A microbial composition, characterized in that, The components include the following components in parts by weight: Pseudomonas fluorescens 6-15 parts, Bacillus subtilis 5-10 parts, Trichoderma harzianum 5-10 parts, Bacillus amyloliquefaciens 15-20 parts, Bacillus megaterium 8-12 parts, Streptomyces 3-6 parts, nitrogen-fixing bacteria 5-10 parts, and potassium-dissolving bacteria 1-3 parts.
2. The microbial composition of claim 1, wherein, The potassium-dissolving bacteria include Bacillus mucilaginosus or Bacillus circulans. The nitrogen-fixing bacteria include any two or several of Bradyrhizobium tropic, Sinorhizobium, Rhizobium leguminosarum, and Azotobacter chroococcum.
3. The microbial composition of claim 1, wherein, When the nitrogen-fixing bacteria are Rhizobium leguminosarum and Azotobacter chroococcum, the mass ratio of the Rhizobium leguminosarum to the Azotobacter chroococcum is (1-1.5):
1.
4. Use of the microbial composition of any one of claims 1-3 in the preparation of a growth-promoting microbial agent for promoting the growth and development of rice seedlings.
5. Use of the microbial composition of any one of claims 1-3 in the preparation of a growth-promoting fertilizer for promoting the growth and development of rice seedlings.
6. A rice seedling growth promoting bacterial agent, characterized by, The microbial composition of any one of claims 1-3.
7. The rice seedling growth promoting bacterial agent according to claim 6, characterized by, The effective viable cell number in the rice seedling growth promoting microbial agent is ≥1.2×10 10 cfu / mL.
8. The method for preparing the rice seedling growth promoting bacterial agent of claim 6, characterized by, The method comprises the following steps: Pseudomonas fluorescens, Bacillus subtilis, Trichoderma harzianum, Bacillus amyloliquefaciens, Bacillus megaterium, Streptomyces, nitrogen-fixing bacteria, and potassium-dissolving bacteria are inoculated and cultured to obtain bacterial suspensions, and then the bacterial suspensions are inoculated into a fermentation medium to perform fermentation culture, thereby obtaining a rice seedling growth-promoting microbial agent.
9. The preparation method according to claim 8, characterized in that, The pH of the fermentation medium is 6.5-7.
3.
10. The preparation method according to claim 8, characterized in that, The fermentation culture is performed for 4-6 days at a temperature of 28-33°C.