Synthetic gene of coumarin in black bamboos and application of synthetic gene
By cloning and overexpressing the PnMYB4 and PnMYB4-1 genes from *Phyllostachys edulis*, the problem of coumarin synthesis regulation in *Phyllostachys edulis* was solved, resulting in a significant increase in coumarin content in *Arabidopsis thaliana* and promoting the regulation of coumarin synthesis pathways.
CN120905234APending Publication Date: 2025-11-07ZHEJIANG FORESTRY UNIVERSITY
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Patent Information
- Application Number
- CN202510822289.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2025-06-12
- Filing Date
- 2025-06-19
- Publication Date
- 2025-11-07
AI Technical Summary
Technical Problem
The lack of in-depth research on the synthesis mechanism of coumarin in purple bamboo in the existing technology makes it difficult to effectively regulate and increase the coumarin content.
Method used
The PnMYB4 and PnMYB4-1 genes from *Phyllostachys edulis* were cloned and validated. By overexpressing these genes in *Arabidopsis thaliana*, recombinant vectors and engineered bacteria were constructed to regulate coumarin synthesis.
Benefits of technology
It significantly increased the content of coumarin in Arabidopsis thaliana, especially the content of scopolamine and scopolamine glycoside, and upregulated the expression level of related synthetic genes, thus promoting the coumarin synthesis pathway.
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Abstract
The invention discloses a coumarin synthetic gene in black bamboo and application thereof, and belongs to the technical field of molecular biology. The invention provides transcription factors PnMYB4 and PnMYB4-1 for promoting synthesis of coumarin in black bamboos and application of the transcription factors PnMYB4 and PnMYB4-1. The PnMYB4 and the PnMYB4-1 belong to an MYB transcription factor family, the length of a complete coding frame of the PnMYB4 is 762 basic groups, and the coded protein contains 253 amino acids; the length of a complete coding frame of the PnMYB4-1 is 744 basic groups, and the coded protein contains 253 amino acids. The coumarin substances in arabidopsis thaliana, which are respectively overexpressed by PnMYB4 and PnMYB4-1, are obviously increased, and in addition, the expression levels of coumarin synthetic genes such as AtPAL1, At4CL1 and AtC4H in overexpressed plants are obviously increased.
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