A method for simultaneously detecting 7 components of Lidan Zhitong tablets based on wavelength switching

Abstract

The application belongs to the technical field of Chinese medicine chemical component content detection, and particularly relates to a method for simultaneously detecting seven components in Lidan Zhitong tablets based on wavelength switching. The method adopts 70% methanol ultrasonic extraction of the test product, adopts a C18 column as a stationary phase, methanol-0.2% phosphoric acid as a mobile phase, realizes good separation of (R,S)-adonirubin, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin and ammonium glycyrrhizinate through gradient elution combined with wavelength switching, and has good linearity, a detection limit and a quantitative limit. The method is simple in operation, can simultaneously quantitatively detect seven active substances in the Lidan Zhitong tablets, improves the quality control level and is beneficial to popularization.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of Chinese medicine chemical component content detection, and particularly relates to a method for simultaneously detecting seven components in Lidan Zhongtong tablets based on wavelength switching. BACKGROUND

[0002] Lidan Zhongtong tablets are compound preparations prepared from 12 Chinese medicines, including Radix Bupleuri, Radix Paeoniae Rubra, Fructus Aurantii, Radix Glycyrrhizae, Herba Artemisiae Scopariae, Rhizoma Corydalis, Radix Atractylodis, Fructus Xanthii, Herba Impatientis, Radix Isatidis, Herba Taraxaci and Rhizoma Curcumae, and have the effects of clearing heat and promoting bile secretion and regulating qi and relieving pain. Lidan Zhongtong tablets are used for treating hypochondriac pain and jaundice (such as acute and chronic hepatitis and cholecystitis) caused by damp-heat of liver and gallbladder. Modern pharmacological researches conducted in China have confirmed that Lidan Zhongtong tablets have the related pharmacological activities and effects of protecting liver, anti-inflammation, analgesia and regulating cholesterol (Zhang Liqun, et al. Pharmacodynamic study of Lidan Zhongtong tablets [J]. Yunnan Journal of Chinese Medicine and Pharmacy, 2012, 33 (8): 55-58).

[0003] The original quality standard of Lidan Zhongtong tablets is recorded in the Eleventh Volume of Traditional Chinese Medicine Prescriptions issued by the Ministry of Health of the People's Republic of China, and has simple physicochemical identification items but no content determination item, so that effective quality control and monitoring cannot be performed. Although quantitative detection researches on Lidan Zhongtong tablets have been carried out in recent years, due to the unknown nature of chemical components (especially active components) of the compound preparations and the mutual interference of complex components, only a single-component liquid chromatography analysis method for paeoniflorin in Lidan Zhongtong tablets has been established. Due to the interference of a large number of unknown components, a complex pretreatment program including a complex purification step of a multi-solvent system is required (Fu Yue, et al. Research on quality standard of Lidan Zhongtong tablets [J]. Chinese Patent Medicines, 2005, 27 (4): 411-413).

[0004] In view of this, there is an urgent need in the field to establish a detection method for simultaneous quality control of multiple components of Lidan Zhongtong tablets to improve the quality control and monitoring level. SUMMARY

[0005] In view of the above problems, the present application found in the previous research that there are seven components in Lidan Zhongtong tablet finished preparations, including (R, S)-adipocytokinins, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin and ammonium glycyrrhizinate. Among them, the published research results show that (R, S)-adipocytokinins have anti-inflammatory and anti-viral activities; paeoniflorin has analgesic effect; chicoric acid has anti-inflammatory, anti-viral and inhibitory effect on secondary liver damage caused by inflammation; neohesperidin has antioxidant and blood lipid regulating activities; chlorogenic acid, naringin and ammonium glycyrrhizinate have anti-inflammatory, liver protection or inhibitory effect on liver damage.

[0006] In view of the above ingredients and the known pharmacological activity and efficacy of the Dantie Aitong tablets, it is suitable as a quality control ingredient of the Dantie Aitong tablets. The purpose of the present application is to provide a method for simultaneously detecting seven components of the Dantie Aitong tablets based on wavelength switching, so as to realize the simultaneous quantitative detection of seven components in the Dantie Aitong tablets, including (R, S)-adipocire, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate. The technical scheme of the present application is as follows:

[0007] A method for simultaneously detecting seven components of the Dantie Aitong tablets based on wavelength switching, the method is a liquid chromatography method, the liquid chromatography method comprises the following steps:

[0008] Preparation of test sample solution: take the test sample, remove the sugar coating, grind into powder, add 70% methanol, ultrasonic extraction, filter, and prepare the test sample solution;

[0009] Preparation of control sample solution: take (R, S)-adipocire, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate control sample, add 70% methanol, and prepare the control sample solution;

[0010] Sample injection and detection: respectively take the control sample solution and the test sample solution into the liquid chromatograph for detection, draw the standard curve, and calculate the content of the seven components; the chromatographic column of the liquid chromatograph is a C18 column, and the column length, inner diameter, and particle size of the chromatographic column are 250mm, 4.6mm, and 5μm respectively; methanol is used as the mobile phase A, 0.2% phosphoric acid is used as the mobile phase B, the flow rate is 1.0mL / min, gradient elution is carried out, and the flow phase gradient elution program is as follows:

[0011]

[0012] The above gradient elution program can also be expressed as: 0~6min, 10% methanol; 6~11min, 10%→20% methanol; 11~20min, 20%→24% methanol; 20~30min, 24%→30% methanol; 30~53min, 30%→44% methanol; 53~55min, 44%→60% methanol; 55~72min, 60%→80% methanol; 72~73min, 80%→10% methanol; 73~80min, 10% methanol.

[0013] The detection wavelength of the liquid chromatography method is as follows:

[0014]

[0015] In combination with the elution procedure of the mobile phase, the above wavelength switching procedure can also be expressed as: 0~13min, 245nm; 13~23min, 327nm; 23~30min, 230nm; 30~40min, 327nm; 40~60min, 283nm; 60~80min, 237nm. In other words, in the wavelength switching procedure, the time points in the time column are the time points of wavelength switching, and no wavelength switching occurs at the time points not listed.

[0016] Preferably, when the test sample solution is prepared, the ultrasonic power is 300W, and the ultrasonic extraction time is 45min~80min.

[0017] Preferably, when the test sample solution is prepared, the ultrasonic frequency is 50kHz.

[0018] Preferably, the concentration of the test sample solution is 0.3g~0.8g of test sample per 25mL of test sample solution. Further preferably, the concentration of the test sample solution is 0.4g~0.6g of test sample per 25mL of test sample solution.

[0019] Preferably, the control solution contains (R,S)-ademetionine, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate at 2μg, 20μg, 80μg, 10μg, 100μg, 90μg, and 20μg per 1mL of the control solution, respectively.

[0020] Preferably, the column temperature of the liquid chromatography is 30℃.

[0021] Preferably, the injection volume of the liquid chromatography is 10µL.

[0022] Preferably, the chromatograph used in the liquid chromatography is an Agilent 1260Ⅱhigh-performance liquid chromatograph.

[0023] Preferably, the C18 column used in the liquid chromatography is a Shimadzu Shim-pack GIST C18 column.

[0024] Beneficial effects

[0025] (1) Compared with the prior art, the detection method of the present application greatly simplifies the test sample extraction method, and the chromatographic separation effect of each active substance is good and suitable for quantitative analysis of multiple components.

[0026] (2) Compared with the prior art, the detection method of the present application can simultaneously quantitatively detect up to 7 active substances in the Lidan Zhitong tablets, thereby improving the quality control level of the Lidan Zhitong tablets, facilitating quality control and drug supervision. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 Chromatogram of the control sample for Example 1;

[0028] Figure 2 Chromatogram of the test sample for Example 1;

[0029] Figure 3 Chromatogram of the test sample for Example 2 with methanol as the extraction solvent;

[0030] Figure 4 Chromatogram of the test sample for Example 2 with ethanol as the extraction solvent;

[0031] Figure 5 Chromatogram of the test sample for Example 2 with 50% methanol as the extraction solvent;

[0032] Figure 6 Chromatogram of the test sample for Example 2 with 70% methanol as the extraction solvent;

[0033] Figure 7 Chromatogram of the test sample for Example 2 with water as the extraction solvent;

[0034] Figure 8 Chromatogram of the test sample for Example 3 with methanol-water as the mobile phase for gradient elution;

[0035] Figure 9 Chromatogram of the test sample for Example 3 with methanol-1% glacial acetic acid as the mobile phase for gradient elution;

[0036] Figure 10 Chromatogram of the test sample for Example 3 with acetonitrile-0.2% phosphoric acid as the mobile phase for gradient elution;

[0037] Figure 11 Chromatogram of the test sample for Example 3 with methanol-0.2% phosphoric acid as the mobile phase for gradient elution. DETAILED DESCRIPTION

[0038] The schemes and effects of the present application will be further described below in conjunction with specific examples. It should be understood that the specific examples are only used to explain the present application and are not used to limit the protection scope of the present application.

[0039] The instruments, reagents, sample sources and models used in the following examples and exploratory examples are as follows:

[0040] Instruments: Agilent 1260 II high-performance liquid chromatograph (Agilent Technologies Co., Ltd.); MS105DU electronic balance (Mettler Toledo Technology (China) Co., Ltd.); XPR10 electronic balance (Mettler Toledo Technology (China) Co., Ltd.); Model 9960D ultrasonic instrument (CBL Photoelectron Technology Co. Ltd.).

[0041] Reagents: (R, S)-ademetionine (batch number: 111753-202408, content 100.0%), chlorogenic acid (batch number: 110753-202119, content 96.3%), paeoniflorin (batch number: 110736-202548, content 98.9%), chicoric acid (batch number: 111752-202105, content 98.3%), naringin (batch number: 110722-202417, content 96.1%), neohesperidin (batch number: 111857-202305, content 99.6%), ammonium glycyrrhizinate (batch number: 110731-202423, content 93.2%) were purchased from China Institute for Drug Control, and experimental water was ultrapure water, methanol and phosphoric acid were chromatographically pure, and other reagents were analytically pure.

[0042] Sample: Lidan Zhongtong tablets, Jilin Junhong Pharmaceutical Co., Ltd.

[0043] Example 1: Method for simultaneous determination of 7 components in Lidan Zhongtong tablets by HPLC wavelength switching method

[0044] This example demonstrates a chromatographic method (sample preparation and detection conditions) for simultaneous determination of 7 components in Lidan Zhongtong tablets based on HPLC wavelength switching method. The sample preparation and detection conditions of this method are as follows:

[0045] (1) Preparation of test solution: Take the test sample, remove the sugar coating, grind into powder, pass through a No. 5 sieve, take about 0.5 g, accurately weigh, place in a conical flask, accurately add 25 mL of 70% methanol, weigh, ultrasonic treat (power 300 W, frequency 50 kHz) for 45 min, cool, re-weigh, add 70% methanol to make up for the weight loss, shake well, filter, and take the filtrate, which is obtained.

[0046] (2) Preparation of control solution (i.e. mixed control solution): Take the appropriate amount of control sample, accurately weigh, add 70% methanol to prepare a solution containing (R, S)-ademetionine, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate at 2 μg, 20 μg, 80 μg, 10 μg, 100 μg, 90 μg, and 20 μg per 1 mL, respectively, which is obtained.

[0047] (3) Chromatographic conditions: The chromatographic column is Shim-pack GIST C18 (250 x 4.6 mm, 5 µm), methanol is used as mobile phase A, and 0.2% phosphoric acid is used as mobile phase B, gradient elution is performed according to Table 1, the flow rate is 1.0 mL / min, the column temperature is 30°C, the injection volume is 10 µL, and the detection wavelength switching program is shown in Table 2. The chromatograms of the control sample and the test sample are shown in Figure 1 , Figure 2 .

[0048] Table 1 Gradient elution procedure

[0049]

[0050] Table 2 Detection wavelength switching procedure

[0051]

[0052] By Figure 1 , Figure 2 It can be seen that under this method, each target substance is well separated, suitable for qualitative and quantitative detection.

[0053] Example 2 Methodology investigation of simultaneous detection of 7 components in Lidan Zhitong tablets by HPLC wavelength switching method and sample determination

[0054] This example demonstrates the methodology investigation research content for the method described in Example 1.

[0055] 1. Investigation and results of extraction solvent

[0056] Take the test product, remove the sugar coating, grind into powder, pass through a No. 5 sieve, take about 0.50 g, accurately weigh 5 portions, respectively, add methanol, ethanol, 50% methanol, 70% methanol, water 50 mL respectively, weigh the weight, ultrasonic treatment (power 300 W, frequency 50 kHz) for 60 min, cool down, weigh again, make up the weight lost with 70% methanol, shake well, filter through a 0.45 μm filter membrane, take 10 μL of the filtrate and inject it into the liquid chromatograph, the chromatographic conditions are the same as in Example 1, and record the chromatogram. The results are shown in Table 3 and Figures 3-7 The results show that with 70% methanol extraction, each component is extracted sufficiently, and the peak shape is good, and each separation degree meets the requirements, so 70% methanol is selected as the extraction solvent.

[0057] Table 3 Investigation results of different extraction solvents (peak area value)

[0058]

[0059] 2. Investigation and results of extraction method and extraction time

[0060] Take the test product, remove the sugar coating, grind into powder, pass through a No. 5 sieve, take about 0.50 g, accurately weigh (5 parts, each about 0.50 g). 3 of them are placed in conical flasks, 2 are placed in flasks, 25 mL of 70% methanol is added, and the weight is weighed. The samples in the conical flasks are ultrasonically extracted (power 300 W, frequency 50 kHz) for 30 min, 45 min and 60 min respectively; the samples in the flasks are heated to reflux for 30 min and 45 min respectively; cool, reweigh, make up the weight lost with 70% methanol, shake well, filter through a 0.45 μm filter membrane, take 10 μL of the filtrate and inject it into the liquid chromatograph, the chromatographic conditions are the same as in Example 1, and the chromatogram is recorded. The test results are shown in Table 4, which shows that the ultrasonic extraction and the heating reflux extraction have similar effects, and the component content does not increase substantially after 45 min of ultrasonic extraction. Therefore, the extraction method is ultrasonic extraction for 45 min.

[0061] Table 4: Results of extraction method and extraction time investigation (peak area value)

[0062]

[0063] 3. Linear and range investigation results

[0064] Take the control sample solution prepared according to the method of Example 1, dilute it step by step to a series of concentrations, and inject it into the sample according to the chromatographic conditions of Example 1. Regress the control sample concentration as the abscissa (X) and the peak area as the ordinate (Y) to draw a standard curve, the results of which are shown in Table 5. From the results, it can be seen that each component has a good linear relationship within its respective range.

[0065] Table 5: Linear relationship and linear range of each component

[0066]

[0067] 4. Precision test and results

[0068] Take the control sample solution prepared according to the method of Example 1, and inject it continuously for 6 times, and inject it into the sample according to the chromatographic conditions of Example 1. The retention time RSD (n=6) of (R,S)-aduncol, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin and ammonium glycyrrhizinate is 0.2%~1.3%; the peak area RSD (n=6) is 0.3%~1.7% (results shown in Table 6, Table 7). The results show that the instrument precision is good.

[0069] Table 6: Retention time RSD

[0070]

[0071] Table 7: Peak area RSD

[0072]

[0073] 5. Repetitive test and results

[0074] Prepare 6 portions of test sample solution according to the method of Example 1, inject into the liquid chromatograph, and determine according to the chromatographic conditions of Example 1. Calculate the peak area RSD (n = 6) of (R, S)-jodopyronychlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate. The results are 0.5% to 1.9% (see Table 8 for results). The results show that the method has good repeatability.

[0075] Table 8. Results of repeatability test

[0076]

[0077] 6. Quantification limit and detection limit

[0078] Dilute the control sample solution prepared according to the method of Example 1 step by step, inject into the liquid chromatograph according to the chromatographic conditions of Example 1, and record the chromatogram. The concentration corresponding to a signal-to-noise ratio S / N of 3 is the detection limit, and the concentration corresponding to a signal-to-noise ratio S / N of 10 is the quantification limit. The detection limits of (R, S)-jodopyronychlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate are 0.011 µg / mL, 0.023 µg / mL, 0.109 µg / mL, 0.024 µg / mL, 0.054 µg / mL, 0.024 µg / mL, and 0.148 µg / mL, respectively; and the quantification limits are 0.032 µg / mL, 0.068 µg / mL, 0.381 µg / mL, 0.118 µg / mL, 0.180 µg / mL, 0.094 µg / mL, and 0.262 µg / mL, respectively.

[0079] 7. Sample injection recovery test and results

[0080] Take 6 conical flask, respectively, the amount of precision to join the control solution prepared by the method of example 1, water bath to dry the solvent, and then take about 0.5 g of the product powder (test sample powder, prepared by the method of example 1), precision weighing, placed in the above conical flask, precision to join 25 mL of 70% methanol, weighing, ultrasonic treatment (power 300 W, frequency 50 kHz) for 45 min, cool, weigh again, make up the weight loss with 70% methanol, shake well, filter, take 10 μL of the filtrate into the liquid chromatograph, according to the chromatographic conditions of example 1, sample determination, determination of the peak area of each component, calculate the recovery. The average recovery of (R, S)-adjujuncin, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate was 95.2%, 98.1%, 98.2%, 97.7%, 97.3%, 99.2%, and 97.9%, respectively, and the RSD was 1.58%, 1.24%, 1.50%, 1.32%, 1.11%, 1.19%, and 1.77%, respectively (see Table 9), indicating that the method has good accuracy.

[0081] Table 9: Recovery rate experiment

[0082]

[0083]

[0084] 8. Sample detection and results

[0085] Take 3 batches of samples, prepare the test sample solution according to the method of example 1, and sample determination according to the chromatographic conditions of example 1. The results are shown in Table 10. The content of each target substance in the 3 batches of samples fluctuates slightly, but the difference is small.

[0086] Table 10: Sample content determination results

[0087]

[0088] Example 3: Method for simultaneous detection of 7 components in Dianzhitongpi tablets by HPLC wavelength switching

[0089] The following is a display of some of the exploration examples in the process of establishing the detection method of the present application. Each exploration example shows the test sample chromatogram under the optimal gradient elution program, wavelength switching program, and flow rate of the mobile phase.

[0090] The test sample preparation method of the exploration example is the same as that of Example 1, the flow rate of the mobile phase is 1.0 mL / min, and the column temperature is 30°C. The detection instrument is an Agilent 1260 II high-performance liquid chromatograph (Agilent Technologies), the chromatographic column is Shim-pack GIST C18 (250 x 4.6 mm, 5 µm), and the injection volume is 10 µL. The results are shown in Tables 1 to 3, respectively. Figures 8-11 .

[0091] Exploration Example 1: Figure 8 The mobile phase used is methanol-water, methanol is mobile phase A, and water is mobile phase B, gradient elution is carried out, and the mobile phase gradient elution program is shown in Table 11.

[0092] Table 11 Gradient elution program of exploration example 1

[0093]

[0094] Figure 8 The wavelength switching program corresponding to Table 11 is: 0~13min 245nm; 13~23min 327nm; 23~30min 230nm; 30~40min 327nm; 40~60min 283nm; 60~80min 237nm. From the above results, it can be seen that under the detection method, chlorogenic acid, chicoric acid, and ammonium glycyrrhizinate do not peak. Figure 8

[0095] Exploration Example 2: Figure 9 The mobile phase used is methanol-1% glacial acetic acid, methanol is mobile phase A, and 1% glacial acetic acid is mobile phase B, gradient elution is carried out, and the mobile phase gradient elution program is shown in Table 12.

[0096] Table 12 Gradient elution program of exploration example 2

[0097]

[0098] Figure 9 The wavelength switching program corresponding to Table 12 is: 0~13min 245nm; 13~23min 327nm; 23~30min 230nm; 30~40min 327nm; 40~60min 283nm; 60~80min 237nm. From the above results, it can be seen that under the detection method, paeoniflorin and chicoric acid peaks are not separated, and ammonium glycyrrhizinate does not peak. Figure 9

[0099] Exploration Example 3: Figure 10 The mobile phase used is acetonitrile-0.2% phosphoric acid, acetonitrile is mobile phase A, and 0.2% phosphoric acid is mobile phase B, gradient elution is carried out, and the mobile phase gradient elution program is shown in Table 13.

[0100] ​​Table 13 Gradient elution procedure of the exploratory example 3

[0101]

[0102] Figure 10 And the corresponding wavelength switching procedure of Table 13 is 0~16min 245nm; 16~23min 327nm; 23~35min 230nm; 35~45min 327nm; 45~60min 283nm; 60~80min 237nm. From the above, it can be seen that the detection method under the condition, paeoniflorin, chicoric acid and other components and adjacent unknown peak resolution does not meet the requirements, it is difficult to be used for quantitative analysis. Figure 10 It can be seen that under the detection method, the separation degree of paeoniflorin, chicoric acid and other components and adjacent unknown peaks does not meet the requirements, and it is difficult to be used for quantitative analysis.

[0103] Exploratory example 4: Figure 11 The mobile phase used is methanol-0.2% phosphoric acid, methanol as mobile phase A, 0.2% phosphoric acid as mobile phase B, gradient elution, and the mobile phase gradient elution procedure is shown in Table 14.

[0104] Table 14 Gradient elution procedure of the exploratory example 4

[0105]

[0106] Figure 11 And the corresponding wavelength switching procedure of Table 14 is 0~13min 245nm; 13~21min 327nm; 21~28min 230nm; 30 28~36min 327nm; 36~55min 283nm; 55~70min 237nm. From the above, it can be seen that the detection method under the condition, chicoric acid and other components and adjacent unknown peak resolution does not meet the requirements, it is difficult to be used for quantitative analysis. Figure 11 It can be seen that under the detection method, the separation degree of paeoniflorin, chicoric acid and other components and adjacent unknown peaks does not meet the requirements, and it is difficult to be used for quantitative analysis.

[0107] The detection method of the present application is a multi-component simultaneous quantitative analysis method of Lidan Zhitong tablets obtained by a large amount of exploratory analysis. Through the comparison of the above examples and exploratory examples, compared with other detection methods, the detection method of the present application realizes the simultaneous quantitative determination of multiple active substances in Lidan Zhitong tablets, which not only improves the quality control and monitoring level, but also simplifies the operation of quality detection, and is conducive to popularization and promotion.

Claims

1. A method for simultaneous detection of 7 components in Lidan Zhitong tablets based on wavelength switching, characterized in that, The method is a liquid chromatography method comprising the following steps: Preparation of the test sample solution: take the test sample, remove the sugar coating, grind into powder, add 70% methanol, ultrasonic extraction, filter, and prepare the test sample solution; the concentration of the test sample solution is 0.3g-0.8g of the test sample per 25mL of the test sample solution; Preparation of the control sample solution: take the (R,S)-adjuvant, chlorogenic acid, paeoniflorin, chicoric acid, naringin, neohesperidin, and ammonium glycyrrhizinate control samples, add 70% methanol, and prepare the control sample solution; the control sample solution contains 2ug of (R,S)-adjuvant, 20ug of chlorogenic acid, 80ug of paeoniflorin, 10ug of chicoric acid, 100ug of naringin, 90ug of neohesperidin, and 20ug of ammonium glycyrrhizinate per 1mL; Injection detection: take the control sample solution and the test sample solution respectively, inject them into the liquid chromatograph for detection, draw the standard curve, and calculate the content of the seven components; the chromatographic column of the liquid chromatograph is a C18 column, and the column length, inner diameter, and particle size of the chromatographic column are 250mm, 4.6mm, and 5um respectively; methanol is used as the mobile phase A, 0.2% phosphoric acid is used as the mobile phase B, the flow rate is 1.0mL / min, gradient elution is performed, and the flow phase gradient elution program is as follows: ； The detection wavelength switching program of the liquid chromatography method is as follows: 。 2. The method for simultaneous detection of 7 components in Lidan Zhitong tablets according to claim 1, characterized in that, When preparing the test sample solution, the ultrasonic power is 300W, and the ultrasonic extraction time is 45min-80min.

3. The method for simultaneous detection of 7 components in Lidan Zhitong tablets according to claim 2, characterized in that, When preparing the test sample solution, the ultrasonic frequency is 50kHz.

4. The method according to claim 1, wherein the method is characterized in that, The concentration of the test sample solution is 0.4g-0.6g of the test sample per 25mL of the test sample solution.

5. The method according to claim 1, wherein the method is characterized in that, The column temperature of the liquid chromatography method is 30℃.

6. The method according to claim 1, wherein the 7 components of Lidan Zhitong tablets are detected simultaneously by wavelength switching. The injection amount of the liquid chromatography method is 10µL.

7. The method according to claim 1, wherein the method is characterized in that, The chromatograph used in the liquid chromatography method is an Agilent 1260Ⅱ high-performance liquid chromatograph.

8. The method according to claim 1, wherein the method is based on wavelength switching for simultaneous detection of 7 components of Lidan Zhitong tablets. The C18 column used in the liquid chromatography method is a Shimadzu Shim-pack GIST C18 column.

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