Crop growth promoter as well as preparation method and application thereof

Abstract

The invention discloses a crop growth promoter as well as a preparation method and application thereof, and belongs to the technical field of composite microbial preparations. The invention provides a crop growth promoter. The crop growth promoter comprises independently packaged straws, a microbial liquid and an organic nitrogen source, the microbial bacterial liquid is a bacillus subtilis bacterial liquid, and the concentration of bacillus subtilis in the bacillus subtilis bacterial liquid is 2.0 * 10 < 8 > cfu / mL to 4.0 * 10 < 8 > cfu / mL; the ratio of the mass of the straw to the volume of the microbial liquid to the mass of the organic nitrogen source is (16-27) g: (5-6) mL: (6-7) g. The crop growth promoter can promote colonization of microorganisms in soil, after colonization of the microorganisms, straw, the microorganisms and an organic nitrogen source interact to promote growth of crops, and the phenomenon that the microorganisms and secondary stubble crops form competitive growth after returning to the field can be avoided.

Description

Technical Field

[0001] This invention belongs to the field of compound microbial preparation technology, specifically relating to a crop growth promoter, its preparation method, and its application. Background Technology

[0002] Returning straw to the field is an important way to increase the organic carbon pool in farmland soil. However, simply returning straw to the field results in a slow decomposition rate, which is not conducive to increasing the organic carbon pool in farmland. Currently, the main approach is to add decomposing microbial agents to the straw to accelerate the in-situ microbial decomposition process of the returned straw and improve its nutrient utilization efficiency.

[0003] However, the carbon-nitrogen ratio of gramineous crop straw is high, ranging from approximately 65:1 to 100:1, which is far higher than the optimal carbon-nitrogen ratio range for microorganisms and crops. When using microbial inoculants to promote straw decomposition, competition between microorganisms and the next crop often occurs. This is because after the microorganisms in the inoculant colonize in the soil, they will use the easily decomposable carbon in the straw to multiply in large quantities, thereby stimulating enhanced mineralization and assimilating and fixing inorganic nitrogen in the soil. This causes the straw-decomposing microorganisms to compete with the next crop for nitrogen, resulting in slow straw decomposition, poor emergence of the next crop, and poor growth.

[0004] Therefore, in order to solve the problem that straw-decomposing bacteria can easily cause antagonism between straw-decomposing bacteria and subsequent crops when straw is returned to the field, thus inhibiting the growth of subsequent crops, it has become an urgent problem to be solved in this field to provide a crop growth promoter that can both promote the colonization and growth of straw-decomposing bacteria and avoid antagonistic effects between straw-decomposing bacteria and subsequent crops. Summary of the Invention

[0005] The purpose of this invention is to provide a crop growth promoter that can promote the colonization and growth of straw decomposition-promoting strains while avoiding antagonistic effects between these strains and subsequent crops. This invention provides...

[0006] A crop growth promoter comprising individually packaged straw, microbial inoculum, and an organic nitrogen source;

[0007] The microbial inoculum is Bacillus subtilis inoculum, and the concentration of Bacillus subtilis in the inoculum is 2.0 × 10⁻⁶. 8 cfu / mL~4.0×10 8 cfu / mL;

[0008] The mass ratio of the straw, the volume of the microbial inoculum, and the organic nitrogen source is 16g-27g: 5mL-6mL: 6g-7g.

[0009] Preferably, the Bacillus subtilis in the Bacillus subtilis culture includes Bacillus subtilis strain numbered ACCC 62095.

[0010] Preferably, the organic nitrogen source is a microbial fermentation product, including yeast fermentation products.

[0011] Preferably, the microbial fermentation product comprises the following components: carbon content 23.22%, nitrogen content 8.07%, and the total nutrients, including N, P2O5 and K2O, ≥10.0%, and the pH of the microbial fermentation product is 4.5 to 8.0;

[0012] The total nutrients also include polysaccharides, organic acids, fulvic acid, Ca, Mg and water-insoluble matter;

[0013] The polysaccharide content, calculated as β-glucan + mannan, is ≥1.5%.

[0014] Organic acids, calculated as malic acid + citric acid + succinic acid, have a content ≥1.0%;

[0015] Fulvic acid content ≥30%;

[0016] Ca content + Mg content ≥ 1.0%;

[0017] Water-insoluble matter content ≤5%.

[0018] The present invention also provides a method for preparing the crop growth promoter as described above, comprising the following steps:

[0019] Prepare the microbial culture solution;

[0020] The straw, microbial inoculum, and organic nitrogen source are packaged separately to obtain the crop growth promoter.

[0021] Preferably, the preparation of the microbial inoculum includes preparing the microbial inoculum using freeze-dried quality control microbial strains; the straw includes corn straw.

[0022] The present invention also provides the application of crop growth promoters prepared by the above-described crop growth promoters or preparation methods in promoting crop growth.

[0023] Preferably, the crop includes wheat.

[0024] The present invention also provides a method for promoting crop growth, which uses the crop growth promoter described above, and specifically includes the following steps:

[0025] Straw and microbial inoculum were mixed to obtain a first mixture; the concentration of the microbial inoculum in the straw was 1.0 × 10⁻⁶. 8 cfu / g ~ 5.0 × 108 cfu / g;

[0026] An organic nitrogen source is added to the first mixture, and the mixture is mixed to obtain a second mixture; in the second mixture, the ratio of the sum of the carbon content of straw and organic nitrogen source to the sum of the nitrogen content of straw and organic nitrogen source is 20-30:1;

[0027] The second mixture is returned to the field and incorporated into the soil along with tillage practices, maintaining a field water holding capacity of 55%–65%.

[0028] Preferably, when returning the second mixture to the field, it is applied at a rate of 7000–8000 kg / hm². 2 The proportion of the fertilizer returned to the field should be [amount].

[0029] The beneficial effects of this invention are as follows:

[0030] This invention provides a crop growth promoter, comprising a microbial inoculum and an organic nitrogen source; the microbial inoculum is a Bacillus subtilis inoculum, and the concentration of Bacillus subtilis in the Bacillus subtilis inoculum is 2 × 10⁻⁶. 8 cfu / mL~4.0×10 8 The volume ratio of the microbial inoculum to the organic nitrogen source is 5-6 mL to 6-7 g. This invention combines Bacillus subtilis with an organic nitrogen source, which is a byproduct of industrial processing, thus enhancing the environmental performance of the formulation. The crop growth promoter described in this invention promotes microbial colonization in the soil. After colonization, the interaction between straw, microorganisms, and the organic nitrogen source promotes crop growth. The limitation on the ratio of straw to organic nitrogen source in this invention also prevents competition between microorganisms and the subsequent crop after returning the straw to the field, ensuring optimal crop growth. Attached Figure Description

[0031] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0032] Figure 1 The dynamic changes in the growth of winter wheat in the pot experiment of Example 3 of the present invention;

[0033] Figure 2 The SPAD values ​​of winter wheat at different growth stages were obtained in a pot experiment of Example 3 of this invention.

[0034] Figure 3 The results of winter wheat plant height in the pot experiment of Example 3 of the present invention;

[0035] Figure 4 The results show the dry weight of the aboveground parts and roots of winter wheat in the pot experiment of Example 3 of the present invention. Detailed Implementation

[0036] This invention provides a crop growth promoter comprising individually packaged straw, microbial inoculum, and an organic nitrogen source; the microbial inoculum is Bacillus subtilis inoculum, and the concentration of Bacillus subtilis in the inoculum is 2 × 10⁻⁶. 8 cfu / mL~4.0×10 8 The mass ratio of the straw, the volume of the microbial inoculum, and the organic nitrogen source is 16g-27g: 5mL-6mL: 6g-7g.

[0037] In one embodiment, the Bacillus subtilis strain in the bacterial solution of the present invention is designated ACCC 62095. In another embodiment, the sequence of the Bacillus subtilis is shown in SEQ ID NO.1. In one embodiment, the concentration of Bacillus subtilis in the bacterial solution of the present invention can be 2 × 10⁻⁶. 8 cfu / mL, 2.5×10 8 cfu / mL, 3×10 8 cfu / mL, 3.5×10 8 cfu / mL and 4×10 8Any of the following concentrations: cfu / mL. In one embodiment, the organic nitrogen source of the present invention is a microbial fermentation product, including yeast fermentation products. In another embodiment, the yeast fermentation product is a fermentation metabolite of yeast cultured using molasses; the yeast fermentation product is a product of Angel Yeast (Chifeng) Co., Ltd., with product number 6917790024205. In one embodiment, the microbial fermentation product of the present invention comprises the following components: carbon content 23.22%, nitrogen content 8.07%, and total nutrients, with N, P2O5, and K2O content ≥10.0%; the pH of the microbial fermentation product is 4.5–8.0; the total nutrients also include polysaccharides, organic acids, fulvic acid, Ca, Mg, and water-insoluble matter; the polysaccharide content, calculated as β-glucan + mannan, is ≥1.5%; the organic acid content, calculated as malic acid + citric acid + succinic acid, is ≥1.0%; the fulvic acid content is ≥30%; the Ca + Mg content is ≥1.0%; and the water-insoluble matter content is ≤5%. In one embodiment, the ratio of the mass of straw, the volume of microbial inoculum, and the mass of organic nitrogen source in the crop growth promoter of the present invention can be any one of the following: 16g:5mL:6g, 17g:5mL:6g, 16g:5mL:7g, 17g:5mL:7g, 16g:5.5mL:6g, 17g:5.5mL:6g, 16g:5.5mL:7g, 17g:5.5mL:7g, 16g:6mL:6g, 17g:6mL:6g, 16g:6mL:7g, and 17g:6mL:7g. In a specific embodiment, the crop growth promoter of the present invention includes individually packaged microbial inoculum and organic nitrogen source; the microbial inoculum is Bacillus subtilis inoculum, the strain number of Bacillus subtilis is ACCC 62095, and the concentration of the Bacillus subtilis inoculum is 3.0 × 10⁻⁶. 8 The organic nitrogen source is a yeast fermentation product, containing 23.22% carbon and 8.07% nitrogen. The total nutrients, including N, P₂O₅, and K₂O, are ≥10.0%. The pH of the fermentation product is 4.5–8.0. The total nutrients include polysaccharides, organic acids, fulvic acid, Ca, Mg, and water-insoluble matter. The polysaccharide content (calculated as β-glucan + mannan) is ≥1.5%. The organic acid content (calculated as malic acid + citric acid + succinic acid) is ≥1.0%. The fulvic acid content is ≥30%. The Ca + Mg content is ≥1.0%. The water-insoluble matter content is ≤5%. The ratio of the mass of straw, the volume of the microbial inoculum, and the mass of the organic nitrogen source is 16.36 g: 3 mL: 4.66 g.

[0038]

[0039] This invention also provides a method for preparing a crop growth promoter, comprising the following steps:

[0040] Prepare the microbial inoculum solution; package the straw, microbial inoculum solution, and organic nitrogen source separately to obtain the crop growth promoter.

[0041] In one embodiment, the straw mentioned in this invention includes crop straw. In another embodiment, the crop straw is corn straw. In one embodiment, the preparation of the microbial inoculum in this invention includes preparing the microbial inoculum using freeze-dried quality control microbial strains. In one embodiment, the step of preparing the microbial inoculum using freeze-dried quality control microbial strains includes: adding LB liquid medium to the freeze-dried quality control microbial strains, mixing, and preparing a powder-water suspension; adding the powder-water suspension to LB slant medium for resuscitation culture to obtain microbial bacterial growth; inoculating the microbial bacterial growth into LB liquid medium for fermentation culture until the OD value reaches 0.3-0.7 to obtain the microbial inoculum. In one embodiment, the culture conditions for the resuscitation culture are: constant temperature culture at 28℃ for 8-12 hours; the culture conditions for the fermentation culture are: shaking culture at 28℃ and 150 r / min. In another embodiment, the culture time for the resuscitation culture can be any one of 8 hours, 9 hours, 10 hours, 11 hours, and 12 hours. As one implementation method, the OD value after fermentation can be any one of 0.3, 0.4, 0.5, 0.6, and 0.7. In a specific embodiment, 0.3 mL of LB liquid medium is added to the freeze-dried microbial control strain, and the mixture is stirred to obtain a powder-water suspension; the powder-water suspension is added to LB slant medium and cultured at a constant temperature of 28°C for 10 hours to obtain microbial bacterial growth; the microbial bacterial growth is inoculated into LB liquid medium and fermented at 28°C with shaking at 150 r / min until the OD value reaches 0.5, obtaining the microbial bacterial solution with a concentration of 3.0 × 10⁻⁶. 8 cfu / mL.

[0042] The present invention also provides the application of crop growth promoters prepared by the above-described crop growth promoters or preparation methods in promoting crop growth.

[0043] In one embodiment, the straw includes crop straw. In another embodiment, the crop straw includes corn straw. In one embodiment, the crop includes wheat. In another embodiment, the wheat includes winter wheat.

[0044] The present invention also provides a method for promoting crop growth, which uses the crop growth promoter described above, and specifically includes the following steps:

[0045] Straw and microbial inoculum solution were mixed to obtain a first mixture; the concentration of the microbial inoculum solution in the straw was 1×10⁻⁶. 8 cfu / g ~ 5.0 × 10 8 cfu / g; Add an organic nitrogen source to the first mixture, mix, and obtain a second mixture; In the second mixture, the ratio of the sum of carbon content of straw and organic nitrogen source to the sum of nitrogen content of straw and organic nitrogen source is 20-30:1; Return the second mixture to the field, mix it with the topsoil according to the tillage measures, maintain the field water holding capacity at 55%-65%, and then sow the crop.

[0046] In one embodiment, the concentration of the microbial inoculum in the straw is 1×10⁻⁶. 8 cfu / g ~ 5.0 × 10 8 cfu / g; as another embodiment, the concentration of the microbial inoculum in straw can be 1×10⁻⁶. 8 cfu / g, 2×10 8 cfu / g, 3×10 8 cfu / g, 4×10 8 cfu / g and 5×10 8 Any of the following values ​​for CFU / g. The limitations on the composition of the organic nitrogen source are as described above and will not be repeated here. As one embodiment, the ratio of the sum of carbon content of straw and organic nitrogen source to the sum of nitrogen content of straw and organic nitrogen source in the second mixture can be any of 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, and 30:1. As one embodiment, the mixing with the topsoil during tillage can be achieved by deep tillage to incorporate the straw promoter into the soil. As one embodiment, the field water holding capacity can be any of 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, and 65%. As one embodiment, when returning the second mixture to the field, it is applied at a rate of 7000–8000 kg / hm². 2 The straw is returned to the field at a certain ratio. Alternatively, the straw return ratio can be 7000 kg / hm². 2 7100kg / hm 2 7200kg / hm 2 7300kg / hm 2 7400kg / hm 2 7500kg / hm 2 7600 kg / hm 2 7700kg / hm 27800 kg / hm 2 7900kg / hm 2 and 8000 kg / hm 2 Any one of the following. In a specific embodiment, the present invention mixes straw and microbial inoculum to obtain a first mixture; the concentration of the microbial inoculum in the straw is 1×10⁻⁶. 8 CFU / g; then add an organic nitrogen source to the first mixture, mix, and obtain a second mixture; in the second mixture, the ratio of the sum of carbon content of straw and organic nitrogen source to the sum of nitrogen content of straw and organic nitrogen source is 25:1; return the second mixture to the field, mix it into the soil with tillage practices, maintain field water holding capacity at 60%, and return straw to the field at an amount of 8000 kg / hm. 2 .

[0047] To further illustrate the present invention, the following detailed description, in conjunction with embodiments, provides a crop growth promoter, its preparation method, and its application, but these descriptions should not be construed as limiting the scope of protection of the present invention.

[0048] Example 1: A crop growth promoter and its preparation method

[0049] The crop growth promoter consists of individually packaged straw, microbial inoculum, and an organic nitrogen source.

[0050] The microbial inoculum is Bacillus subtilis inoculum, and the concentration of the Bacillus subtilis inoculum is 3.0 × 10⁻⁶. 8 cfu / mL; The gene sequence information of Bacillus subtilis culture is shown in SEQ ID NO.1, and is currently deposited at the National Microbial Culture Collection Center of China, strain number ACCC 62095.

[0051] The ratio of the mass of straw, the volume of microbial inoculum, and the mass of organic nitrogen source is 16.36g: 3mL: 4.66g.

[0052] The biochemical characteristics of the *Bacillus subtilis* strain used in this embodiment are as follows: *Bacillus subtilis* genus, non-type strain, aerobic, Gram-negative, non-mobile, rod-shaped, white colonies with a smooth surface, cell width 0.5–0.7 μm and length 1.3–2.7 μm. It is positive for catalase and methyl red reactions, negative for the VP test, positive for starch and gelatin hydrolysis tests, and positive for nitrate reduction and citrate utilization tests. The suitable culture medium for *Bacillus subtilis* is liquid LB medium, with the following components: 10 g / L peptone, 5 g / L yeast extract, 10 g / L sodium chloride, pH 7.0–7.5. The culture temperature for *Bacillus subtilis* is 28℃.

[0053] The organic nitrogen source is a microbial fermentation product with the following composition: carbon content 23.22%, nitrogen content 8.07%, total nutrients (N+P2O5+K2O≥10.0%) including polysaccharides (calculated as β-glucan+mannan)≥1.5%, organic acids (calculated as malic acid+citric acid+succinic acid)≥1.0%, fulvic acid≥30%, Ca+Mg≥1.0%, water-insoluble matter≤5%, pH4.5~8.0, purchased from Angel Yeast (Chifeng) Co., Ltd., product number: 6917790024205.

[0054] The preparation method of the crop growth promoter is as follows:

[0055] The specific steps for preparing microbial culture using freeze-dried quality control strains are as follows:

[0056] Disinfect the surface of the vial with 75% alcohol, open the vial under aseptic conditions, add 0.3 mL of LB liquid culture medium into the vial, mix, and prepare a powder hydrated suspension.

[0057] The powder hydrate suspension was added to LB slant medium and cultured at 28°C for 12 hours to obtain microbial biofilm;

[0058] The microbial bacterial culture was inoculated into LB liquid medium and fermented at 28°C with shaking at 150 rpm. After reaching the logarithmic growth phase, physiological saline was used as a blank control group to zero the culture. The concentration of the bacterial culture was measured until the OD value of the bacterial culture reached 0.5, at which point the culture was stopped. The culture was then carried out according to the formula 1OD = 1 × 10⁻⁶. 9 The concentration of the original bacterial culture was calculated using CFU / mL, yielding a concentration of 3 × 10⁻⁶. 8 Microbial culture solution with cfu / mL.

[0059] The microbial inoculum and the organic nitrogen source are packaged separately to obtain the crop growth promoter.

[0060] Example 2: A method for promoting the growth of winter wheat

[0061] This embodiment utilizes the microbial inoculum solution described in Example 1, and the steps of the method for promoting winter wheat growth are as follows:

[0062] According to a concentration of 3×10 8 The volume of the microbial inoculum solution (cfu / mL) was mixed with the mass of straw at a ratio of 1 mL:3 g, resulting in an effective concentration of microorganisms in the straw of 1.0 × 10⁻⁶. 8 The first mixture was obtained by adding an organic nitrogen source to the first mixture and mixing it to obtain a second mixture. The ratio of the sum of carbon content of straw and organic nitrogen source to the sum of nitrogen content of straw and organic nitrogen source in the second mixture was 25:1.

[0063] The second mixture is returned to the field and incorporated into the soil along with tillage practices, maintaining a field water holding capacity of 60%; the return to the field is carried out at a ratio of 8000 kg of straw per mu.

[0064] Example 3: Pot Experiment with Crop Growth Promoter

[0065] Soil samples were collected from Fangcheng County, Nanyang City, Henan Province (33°0′16″N, 112°53′14″E). The soil type was sandy black soil. The soil plots used for soil collection adopted a winter wheat-summer maize rotation system. The soil composition was pH 5.80, organic matter 50.05 g / kg, total nitrogen 2.21 g / kg, available phosphorus 90.92 mg / kg, and available potassium 298.00 mg / kg.

[0066] This embodiment uses round pots for potted plant experiments. The round pots are 20cm in diameter and 28cm in depth. A total of 12 round pots are used and divided into 3 groups. The different groups are treated as shown in Table 1. Each group has 3 replicates. The specific operation steps for each treatment are as follows:

[0067] Group T2: Dried corn stalks and microbial inoculum were mixed at a ratio of 16.36 g: 5.5 mL to obtain the first mixture, with a microbial inoculum concentration of 3 × 10⁻⁶. 8 CFU / mL. Add 6.35 g of organic nitrogen source to the first mixture, mix, and obtain the second mixture. Mix the second mixture thoroughly with the soil, place it in a round basin, and adjust the water content to 60% of field capacity. Set aside for later use.

[0068] Group T1: Dried corn stalks and microbial inoculum were mixed at a ratio of 16.36 g: 5.5 mL to obtain the first mixture. The concentration of the microbial inoculum was 3 × 10⁻⁶ g / mL. 8 cfu / mL. Take the first mixture and bury it in a round basin, adjust it to 60% of field capacity, and set aside.

[0069] Group T0: Take 16.36g of dried corn stalks, mix them with the soil, place them in a round basin, and adjust the water content to 60% of the field capacity.

[0070] In the 12 round basins, the soil in each basin, based on dry weight, weighed 1.5 kg, and the amount of straw used in each basin was 16.36 g / basin, equivalent to returning 8000 kg / hm² to the field. 2 Corn stalks.

[0071] Table 1 Treatments in pot experiments

[0072]

[0073] Note: In Table 1, / indicates that the component is not added.

[0074] Groups T0, T1, and T2 were prepared by mixing the raw materials shown in Table 1 with soil. The resulting mixtures were then placed in round pots, with wheat seeds sown on the surface of the mixture at a rate of 15 seeds per pot. A small amount of soil was then used to cover the wheat. Wheat growth was observed after sowing, and photographs were taken of different groups on days 17, 23, and 48 post-sowing. One pot from each group was randomly selected and displayed. The results are shown below. Figure 1 As shown in the figure. The chlorophyll content (SPAD value) of leaves was measured on days 14, 17, 23, 32, and 44 after wheat sowing. The results are as follows. Figure 2 As shown in Table 2. Winter wheat plant height was measured on days 17 and 23 after sowing. To reduce the influence of inconsistent emergence times on plant height data and minimize errors, this embodiment excluded wheat plant height data from extremely early and extremely late emergence in the pot experiment. The remaining wheat plant height data were summarized, and the results are shown in Table 2. Figure 3 As shown in Table 3. Destructive sampling was performed 48 days after sowing to determine the root and aboveground dry weight of wheat in each pot under each treatment. The results are shown in Table 3. Figure 4 As shown in Table 4.

[0075] Table 2. Changes in chlorophyll content (SPAD value) of winter wheat in different groups.

[0076] deal with 14d 17d 23d 32d 44d T0 35.2 35.0 26.6 24.6 21.5 T1 36.5 33.5 27.0 24.2 19.5 T2 42.4 43.1 42.2 43.2 36.2

[0077] Table 3. Plant height (cm) of winter wheat in different groups

[0078]

[0079]

[0080]

[0081] Table 4. Changes in aboveground dry weight and root biomass of winter wheat in different groups (mg / plant)

[0082]

[0083] Note: In Table 4, the average weight of a single straw plant and the average weight of a single root system are both integers; SE represents the standard error.

[0084] Depend on Figure 1 As can be seen, after adding the crop growth promoter prepared in this invention, the growth rate of wheat in groups T1 and T2 was significantly increased compared with group T0, and the plant height and number of leaves were significantly higher in groups T1 and T2 than in group T0. Figures 2-4As shown in Tables 2-4, there was no significant difference in SPAD values ​​of winter wheat leaves at different growth stages under the T1 and T0 treatments. However, the T2 treatment significantly increased the SPAD value of winter wheat leaves compared to the T1 treatment. Furthermore, measurements of winter wheat plant height at 17 and 23 days after sowing also revealed that the T2 treatment significantly increased the plant height compared to both the T1 and T0 treatments. This indicates that the T2 treatment can prevent competition for nitrogen between crops and microorganisms under straw return, and promote straw decomposition and nutrient release to support winter wheat growth. This result was also... Figure 4 It was confirmed that the T2 treatment significantly increased the aboveground dry weight and root dry weight of winter wheat by 125.64%–127.53% and 54.81%–36.37% compared with the T0 and T1 treatments, respectively.

[0085] In summary, the crop growth promoter prepared by this invention comprises individually packaged straw, microbial inoculum, and an organic nitrogen source; the microbial inoculum is Bacillus subtilis inoculum, and the concentration of Bacillus subtilis in the Bacillus subtilis inoculum is 2 × 10⁻⁶. 8 cfu / mL~4.0×10 8 The mass ratio of the straw, the microbial inoculum volume, and the organic nitrogen source is 16g-27g:5mL-6mL:6g-7g. In the crop growth promoter of this invention, the interaction between straw, microbial inoculum, and organic nitrogen source promotes microbial colonization in the field, decomposes straw to return nutrients to the field, and maintains a suitable carbon-nitrogen ratio to prevent microorganisms from competing with subsequent crops for nutrients after colonization, thus affecting the growth of the next crop. The crop growth promoter of this invention has broad application prospects in the planting field.

[0086] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, not all embodiments. People can obtain other embodiments without creative effort, as shown in these embodiments, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A crop growth promoter, characterized in that, This includes individually packaged straw, microbial inoculum, and organic nitrogen sources; The microbial inoculum is Bacillus subtilis inoculum, and the concentration of Bacillus subtilis in the inoculum is 2.0 × 10⁻⁶. 8 cfu / mL~4.0×10 8 cfu / mL; The mass ratio of the straw, the volume of the microbial inoculum, and the organic nitrogen source is 16g-27g: 5mL-6mL: 6g-7g.

2. The crop growth promoter as described in claim 1, characterized in that, The Bacillus subtilis bacterial solution contained Bacillus subtilis strain numbered ACCC 62095.

3. The crop growth promoter as described in claim 1, characterized in that, The organic nitrogen source is a microbial fermentation product, which includes yeast fermentation products.

4. The crop growth promoter as described in claim 3, characterized in that, The microbial fermentation product comprises the following components: carbon content 23.22%, nitrogen content 8.07%, and total nutrients including N, P2O5 and K2O ≥10.0%, and the pH of the microbial fermentation product is 4.5–8.0; The total nutrients also include polysaccharides, organic acids, fulvic acid, Ca, Mg and water-insoluble matter; The polysaccharide content, calculated as β-glucan + mannan, is ≥1.5%. Organic acids, calculated as malic acid + citric acid + succinic acid, have a content ≥1.0%; Fulvic acid content ≥30%; Ca content + Mg content ≥ 1.0%; Water-insoluble matter content ≤5%.

5. The method for preparing the crop growth promoter according to any one of claims 1 to 4, characterized in that, Includes the following steps: Prepare the microbial culture solution; The straw, microbial inoculum, and organic nitrogen source are packaged separately to obtain the crop growth promoter.

6. The preparation method according to claim 5, characterized in that, The preparation of the microbial inoculum includes preparing the microbial inoculum using freeze-dried quality control microbial strains; the straw includes corn straw.

7. The application of the crop growth promoter according to any one of claims 1 to 4 or the crop growth promoter prepared by the preparation method according to any one of claims 5 to 6 in promoting crop growth.

8. The application as described in claim 7, characterized in that, The crop mentioned includes wheat.

9. A method for promoting crop growth, characterized in that, The process involves using the crop growth promoter as described in any one of claims 1 to 4, specifically including the following steps: Straw and microbial inoculum were mixed to obtain a first mixture; the concentration of the microbial inoculum in the straw was 1.0 × 10⁻⁶. 8 cfu / g ~ 5.0 × 10 8 cfu / g; An organic nitrogen source is added to the first mixture, and the mixture is mixed to obtain a second mixture; in the second mixture, the ratio of the sum of the carbon content of straw and organic nitrogen source to the sum of the nitrogen content of straw and organic nitrogen source is 20-30:1; The second mixture is returned to the field and incorporated into the soil along with tillage practices, maintaining a field water holding capacity of 55%–65%.

10. The method as described in claim 9, characterized in that, When returning the second mixture to the field, apply 7000–8000 kg / hm². 2 The proportion of the fertilizer returned to the field should be [amount].

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