Feed for improving animal immunity and preparation method thereof
Through scientific formulation and preparation processes, the prepared feed significantly enhances animal immunity, solving the problems of insignificant immunity enhancement and poor stability of existing feeds, and achieving improvements in animal health and growth performance.
Patent Information
- Application Number
- CN202511378729.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-25
- Publication Date
- 2025-11-11
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Existing feeds are not effective in improving animal immunity and have poor stability in harsh environments, making it difficult to meet the needs of animal husbandry.
The feed is prepared by using a scientifically formulated blend of basic energy feed, basic protein feed, astragalus polysaccharide, yeast β-glucan, Bacillus subtilis, stable vitamin C, vitamin E, zinc methionine, selenium yeast, and specific immune enhancers, through processes such as mixing, activation, and steam conditioning, to achieve synergistic effects of multiple components.
It significantly enhances animal immunity, reduces morbidity, promotes growth and development, improves gut health, strengthens antioxidant protection, and helps animals adapt to the farming environment.
Smart Images
Figure CN120918285A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of functional feed technology, specifically to a feed for improving animal immunity and its preparation method. Background Technology
[0002] In modern animal husbandry, animal health and immunity are crucial. However, existing feed technologies have many shortcomings in enhancing animal immunity. Traditional feed formulations primarily focus on animal growth and production performance, offering limited support and regulation for the immune system. With increasing stocking densities and disease pressures, animals face higher health risks, which not only affect their growth and development but also increase farming costs and economic losses.
[0003] Currently, there is a lack of feed on the market that can effectively enhance animal immunity. While some existing feed additives claim to have immunomodulatory functions, their effects are often not significant enough, or they suffer from poor stability and low bioavailability in practical applications. Furthermore, many immune enhancers are prone to failure under harsh conditions such as high temperature and high humidity, making them unsuitable for the actual needs of the farming environment.
[0004] The livestock industry has an urgent need for feed products that can significantly enhance animal immunity. Such feed not only helps animals resist disease but also reduces antibiotic use to some extent, aligning with current trends in green farming and animal welfare. However, this demand has not yet been fully met, and existing products on the market still have significant room for improvement in terms of ingredient formulation, mechanism of action, and actual effectiveness.
[0005] Therefore, developing a new and efficient feed to enhance animal immunity has become an urgent problem to be solved in the livestock industry. Summary of the Invention
[0006] The purpose of this invention is to address the significant shortcomings of existing technologies in enhancing animal immunity by providing a feed and its preparation method that effectively improves animal immunity, thereby compensating for the deficiencies in the immune regulation function of feeds in existing technologies.
[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A feed for enhancing animal immunity comprises the following ingredients by weight: basal energy feed: 50-70 parts, basal protein feed: 5-15 parts, astragalus polysaccharide: 2-8 parts, yeast β-glucan: 1.5-3 parts, Bacillus subtilis: 2-4 parts, stabilized vitamin C: 0.1-0.3 parts, vitamin E: 0.5-2 parts, zinc methionine: 0.2-0.5 parts, selenium yeast: 0.1-0.5 parts, and immune enhancer: 0.5-2 parts; The immune enhancer is a compound represented by Formula 1: Formula 1; In Formula 1, R1 is selected from: methyl, tert-butyl, methoxy, methyl ester.
[0008] Furthermore, the basic energy feed is at least one of corn, soybean meal, and wheat bran.
[0009] Furthermore, the basic protein feed is at least one of fish meal, meat and bone meal, and rapeseed meal.
[0010] Furthermore, the viable count of the Bacillus subtilis is not less than 1×10⁻⁶. 10 CFU / g.
[0011] Furthermore, the stabilized vitamin C is at least one of ascorbic acid polyphosphate and ascorbic acid-2-phosphate.
[0012] Furthermore, the immune enhancer is selected from one or more combinations of compounds with the structures shown below: ; .
[0013] A method for preparing feed to enhance animal immunity includes the following steps: S1. Crush the basic energy feed and basic protein feed through a 40-60 mesh sieve, mix them evenly, and obtain a premixed material; S2. The stabilized vitamin C, vitamin E, zinc methionine, selenium yeast, immune enhancer and 20% premixed material are mixed to obtain mixture one; S3. Activate the Bacillus subtilis in warm water at 35-37℃ for 15-20 minutes to obtain activated Bacillus subtilis bacterial solution; S4. Add the Astragalus polysaccharide, yeast β-glucan, mixture one, activated Bacillus subtilis bacterial solution and the remaining premixed materials into a mixer and mix thoroughly for 20-30 minutes to obtain mixture two; S5. The mixture is conditioned by steam and then pressed into pellets by a pellet mill to obtain pellet feed; S6. The prepared pelleted feed is dried at a low temperature of ≤50℃, cooled to room temperature and then packaged to obtain a feed that improves animal immunity.
[0014] Furthermore, the amount of warm water used in S3 is 3-5 times the amount of Bacillus subtilis used.
[0015] Furthermore, the steam conditioning temperature in S5 is 75-85℃, and the conditioning time is 30-60 seconds.
[0016] Furthermore, the low-temperature drying temperature in S6 is 45-50°C, until the feed moisture content is below 12%.
[0017] The feed of this invention, through scientific formulation and preparation processes, achieves synergistic effects among its components, effectively enhancing animal immunity, reducing disease risk, and adapting to the farming environment. The complementary effects of multiple components, through immune activation, antioxidant protection, probiotic regulation, and stability enhancement, improve overall immune efficacy.
[0018] Basic energy feeds provide carbohydrates and energy to maintain basal metabolism in animals and fuel immune cell activity. Basic protein feeds provide essential amino acids and proteins to support immunoglobulin synthesis and repair tissue damage. Astragalus polysaccharides, as natural immunomodulators, stimulate macrophage and lymphocyte activity, enhancing non-specific immunity. Yeast β-glucan can activate Toll-like receptors, promoting antibody production and cellular immunity. Bacillus subtilis can colonize the gut, inhibit the growth of harmful bacteria, and secrete enzymes to promote nutrient absorption, improve intestinal barrier function, and enhance local immunity (such as increasing sIgA secretion). Combined with immune enhancers, it addresses systemic immunodeficiency caused by weak gut health. Stable vitamin C and vitamin E, as antioxidants, protect cells from free radical damage and promote leukocyte function, enhancing overall protection. Zinc methionine, as a metalloenzyme cofactor, supports thymus development and antibody production. In synergy with selenium-enhanced yeast, it optimizes trace element balance and strengthens immune cell signal transduction. Immune enhancers, with their structural specificity, possess highly efficient immunomodulatory activity. They can target and regulate immune pathways and enhance cytokine secretion. Combined with polysaccharides and probiotics, it enhances the strength and duration of the overall immune response.
[0019] Compared with the prior art, the beneficial effects of the present invention are: 1. Significantly enhances animal immunity: This invention effectively activates the animal immune system through novel immune enhancers, scientific formulation, and synergistic effects of multiple components, significantly reducing the animal's morbidity and effectively improving the animal's health and disease resistance.
[0020] 2. Enhanced antioxidant protection: The addition of stable vitamin C, vitamin E and other antioxidants to feed can effectively scavenge free radicals, protect immune cells from oxidative damage, further enhance immune function, and improve the health of animals.
[0021] 3. Significantly promotes animal growth and development: By optimizing the nutritional structure and immune regulation function of feed, this invention significantly promotes animal growth and development, increases the average daily weight gain, and thus improves breeding efficiency and economic benefits. Attached Figure Description
[0022] Figure 1This is an NMR image of the immune enhancer 1 described in this invention. Detailed Implementation
[0023] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are merely some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0024] Synthesis example 1 Synthesis of immune enhancer 1: ; A1: Under a nitrogen atmosphere, 20 g of raw material 1, 18.09 g of raw material 2, and 200 g of toluene solution were added to the reaction system. 18.11 g of sodium tert-butoxide, 0.21 g of palladium acetate, and 0.95 g of tri-tert-butylphosphide were also added to the reaction system. The mixture was stirred until homogeneous, heated to 120 °C, and refluxed for 12 h. After the reaction was complete, the temperature was slightly lowered, and the mixture was filtered using diatomaceous earth to remove salts and catalysts. The filtrate was cooled to room temperature, washed three times with water, and the organic phase was retained. The aqueous phase was then extracted with ethyl acetate. The combined organic phases were dried with anhydrous magnesium sulfate, and the solvent was removed using a rotary evaporator. The solution was then evaporated to dryness, followed by column chromatography using a mixture of petroleum ether and ethyl acetate as the eluent. The solution was evaporated to dryness to obtain 23.03 g of intermediate 1.
[0025] A2: 23.03 g of intermediate 1 was placed in a double-necked flask, and 300 mL of dichloromethane was added under nitrogen protection. Then, 29.79 g of 2-(7-azabenzotriazole)-N,N,N',N'-tetramethylurea hexafluorophosphate was added, and the mixture was stirred at room temperature for 1 h. 8.63 g of raw material 3 was dissolved in 50 mL of dichloromethane and added to the above system. Then, 9.21 g of N,N-diisopropylethylamine was added under an ice-water bath. The ice-water bath was removed, and the system was stirred at room temperature overnight. For post-treatment, 300 mL of dichloromethane was added, followed by washing three times with 1M hydrochloric acid aqueous solution, three times with saturated sodium bicarbonate aqueous solution, and the organic phase was washed with saturated brine. The mixture was dried over anhydrous magnesium sulfate, filtered, and evaporated to dryness. Column chromatography was performed, eluting with a mixture of petroleum ether and ethyl acetate, and then evaporated to dryness to obtain 23.76 g of immunostimulant 1.
[0026] Structural assessment: Mass spectrometry of intermediate 1 (M / Z MS+1): 324; Mass spectrometry of immune enhancer 1 (M / Z MS+1: 427); NMR of immune enhancer 1: 1H NMR (Chloroform-d)δ9.28(s,1H),7.96(t,1H),7.76-7.65(m,2H),7.54-7.44(m,2H),7.44-7.32 (m,4H),7.30-7.18(m,2H),7.17-7.05(m,3H),7.05-6.99(m,2H),4.70(dt,2H),2.38(d,3H).
[0027] Synthesis Example 2-Synthesis Example 4 In Synthesis Examples 2-4, immunostimulant 2-immunostimulant 4 were synthesized sequentially, following the same synthesis method as in Synthesis Example 1, except that raw material 3 was replaced, while the rest remained the same as in Synthesis Example 1. The specific structures of raw material 3, immunostimulant 2-immunostimulant 4, and mass spectrometry (M / Z MS+1) data are shown in Table 1.
[0028] Table 1. Structures of raw material 3, immune enhancer 2 to immune enhancer 4, and mass spectrometry M / Z MS+1 data involved in Synthesis Examples 2-4.
[0029] Example 1
[0030] Preparation of a feed to enhance animal immunity 1. Raw material ratio: Basic energy feed: 60 parts, selected from a combination of corn and soybean meal, with a mass ratio of 2:1; Basic protein feed: 10 parts, selected from fishmeal; Astragalus polysaccharide: 5 parts, purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd., CAS: 89250-26-0; Yeast β-glucan: 2 portions, purchased from Shandong Yatu Biotechnology Co., Ltd., CAS: 9012-72-0; Bacillus subtilis: 2.5 samples, with a viable count of not less than 1×10⁻⁶. 10 CFU / g, purchased from: Jiangsu Lvke Biotechnology Co., Ltd.; Stable vitamin C: 0.2 parts, selected from ascorbic acid polyphosphate, purchased from: Shaanxi Xihua Chemical Industry Co., Ltd.; Vitamin E: 1 serving, purchased from: Hubei Henglvyuan Technology Co., Ltd.; Zinc methionine: 0.3 parts, purchased from Shandong Xinxiong Biotechnology Co., Ltd., CAS: 56329-42-1; Selenium yeast: 0.3 parts, purchased from Suzhou Laihui Biotechnology Co., Ltd.; Immunostimulant: 1 part, selected from immunostimulant 1 synthesized in Synthesis Example 1. 2. Preparation method: S1. Put the basic energy feed and basic protein feed into the pulverizer for preliminary pulverization. After pulverization, pass the pulverized feed through a 60-mesh sieve. Put the materials into a twin-shaft mixer and mix them evenly to obtain premixed materials.
[0031] S2. Take 14 parts of the premixed material and add it together with stable vitamin C, vitamin E, zinc methionine, selenium yeast and immune enhancer into a conical mixer. Mix at room temperature for 15 minutes to ensure that the trace components are evenly dispersed to obtain mixture one. S3. Add 10 parts of 36°C warm water to Bacillus subtilis and activate it in a constant temperature water bath (temperature set to 36°C) for 18 minutes, stirring continuously, to obtain activated Bacillus subtilis bacterial solution; S4. Add Astragalus polysaccharide, yeast β-glucan, mixture one, activated Bacillus subtilis bacterial solution and the remaining 56 parts of premixed materials into a double cone mixer, and mix thoroughly for 25 minutes at 40 rpm to obtain mixture two. S5. Pass the mixture into a steam conditioner, condition at 80°C for 45 minutes, and then press it into pellets using a ring die pellet mill to obtain pellet feed. S6. Feed the pelleted feed into a low-temperature dryer, set the temperature to 47°C and dry until the moisture content drops below 11%. After drying, cool to room temperature and package to obtain a feed that enhances animal immunity.
[0032] Examples 2-4 The preparation of a feed to enhance animal immunity is carried out by referring to the preparation method of Example 1, except that the immune enhancer is replaced in turn with the immune enhancer synthesized in Synthetic Examples 2-4, and the rest is the same as in Example 1.
[0033] Comparative Example 1 The preparation of a feed to enhance animal immunity is the same as in Example 1, except that the immune enhancer is not added.
[0034] Comparative Example 2 The preparation of a feed to enhance animal immunity is carried out according to the preparation method of Example 1, except that the astragalus polysaccharide is not added, and the rest is the same as in Example 1.
[0035] Comparative Example 3 The preparation of a feed to enhance animal immunity is carried out according to the preparation method of Example 1, except that yeast β-glucan is not added, and the rest is the same as in Example 1.
[0036] Comparative Example 4 The preparation of a feed to enhance animal immunity is carried out according to the preparation method of Example 1, except that Bacillus subtilis is not added, and the rest is the same as in Example 1.
[0037] Comparative Example 5 A feed for enhancing animal immunity was prepared by referring to the preparation method of Example 1, except that the immune enhancer was replaced with comparative compound 1 (structural formula: The rest remains the same as in Example 1.
[0038] Performance testing Healthy weaned Yorkshire piglets were selected and fed with the feeds prepared in Examples 1-4 and Comparative Examples 1-5, respectively. The control group was fed with formulated feed for twin piglets. Each group consisted of 100 piglets, half male and half female, with free access to food and water. The feeding was carried out continuously for 150 days, and the following tests were performed: 1. Record the morbidity rate of piglets. The specific calculation formula is: morbidity rate of piglets = number of sick piglets during the feeding period ÷ total number of piglets × 100%. The results are shown in Table 2.
[0039] 2. Detect various indicators of immune function in piglets (IgG, IgA). The results are shown in Table 2.
[0040] 3. Record the weight of the pigs before and after feeding, and calculate the average daily weight gain of the pigs. The results are shown in Table 2.
[0041] Table 2. Performance test results of a feed for improving animal immunity prepared in the examples and comparative examples.
[0042]
[0043] In the performance test results, the feed formulation of this invention exhibited a significant positive trend: compared to the control and comparative groups, the example group significantly reduced the morbidity rate of piglets, while significantly increasing IgG and IgA levels and promoting an increase in average daily weight gain. When key components such as immune enhancers, astragalus polysaccharides, yeast β-glucan, or Bacillus subtilis were lacking in the feed, the morbidity rate increased, immunoglobulin levels decreased, and daily weight gain was negatively affected. Overall, the specific immune enhancer and synergistic formulation design of this invention effectively enhances the overall immunity and growth performance of animals by optimizing immune activation and gut health.
[0044] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A feed for enhancing animal immunity, characterized in that, It contains the following ingredients by weight: basic energy feed: 50-70 parts, basic protein feed: 5-15 parts, astragalus polysaccharide: 2-8 parts, yeast β-glucan: 1.5-3 parts, Bacillus subtilis: 2-4 parts, stabilized vitamin C: 0.1-0.3 parts, vitamin E: 0.5-2 parts, zinc methionine: 0.2-0.5 parts, selenium yeast: 0.1-0.5 parts, immune enhancer: 0.5-2 parts; The immune enhancer is a compound represented by Formula 1: Formula 1; In Formula 1, R1 is selected from: methyl, tert-butyl, methoxy, methyl ester.
2. The feed for enhancing animal immunity according to claim 1, characterized in that, The basic energy feed is at least one of corn, soybean meal, and wheat bran.
3. The feed for enhancing animal immunity according to claim 1, characterized in that, The basic protein feed is at least one of fish meal, meat and bone meal, and rapeseed meal.
4. The feed for enhancing animal immunity according to claim 1, characterized in that, The viable count of the Bacillus subtilis is not less than 1×10⁻⁶. 10 CFU / g.
5. The feed for enhancing animal immunity according to claim 1, characterized in that, The stabilized vitamin C is at least one of ascorbic acid polyphosphate and ascorbic acid-2-phosphate.
6. The feed for enhancing animal immunity according to claim 1, characterized in that, The immune enhancer is selected from one or more combinations of compounds with the structures shown below: ； 。 7. A method for preparing a feed for enhancing animal immunity according to any one of claims 1-6, characterized in that, Includes the following steps: S1. Crush the basic energy feed and basic protein feed through a 40-60 mesh sieve, mix them evenly, and obtain a premixed material; S2. The stabilized vitamin C, vitamin E, zinc methionine, selenium yeast, immune enhancer and 20% premixed material are mixed to obtain mixture one; S3. Activate the Bacillus subtilis in warm water at 35-37℃ for 15-20 minutes to obtain activated Bacillus subtilis bacterial solution; S4. Add the Astragalus polysaccharide, yeast β-glucan, mixture one, activated Bacillus subtilis bacterial solution and the remaining premixed materials into a mixer and mix thoroughly for 20-30 minutes to obtain mixture two; S5. The mixture is conditioned by steam and then pressed into pellets by a pellet mill to obtain pellet feed; S6. The prepared pelleted feed is dried at a low temperature of ≤50℃, cooled to room temperature and then packaged to obtain a feed that improves animal immunity.
8. The method for preparing feed to enhance animal immunity according to claim 7, characterized in that, The amount of warm water used in S3 is 3-5 times the amount of Bacillus subtilis used.
9. The method for preparing feed to enhance animal immunity according to claim 7, characterized in that, The steam conditioning temperature in S5 is 75-85℃, and the conditioning time is 30-60 seconds.
10. The method for preparing a feed to enhance animal immunity according to claim 7, characterized in that, The low-temperature drying in S6 is at a temperature of 45-50℃, until the moisture content of the feed is below 12%.