5 apos; application of disodium 5 '-ribonucleotide or mannan oligosaccharide as feed additive

Abstract

According to the application of disodium 5 '-ribonucleotide or mannan oligosaccharide as a feed additive, the feed additive is a special feed additive for scylla paramamosain. According to the invention, disodium 5 '-ribonucleotide or mannan oligosaccharide is used as an additive to be applied to the scylla paramamosain feed, so that the weight gain rate of the scylla paramamosain can be remarkably increased, and the malondialdehyde content in the scylla paramamosain body is reduced.

Description

Technical Field

[0001] This invention belongs to the field of feed additive technology, specifically relating to the application of a disodium 5'-flavor nucleotide or mannan oligosaccharide as a feed additive. Background Technology

[0002] The mud crab (Scylla serrata) is the most produced farmed marine crab in my country. However, due to a lack of comprehensive nutritional requirements data, there is currently a shortage of high-quality artificial pelleted feed on the market, severely hindering the sustainable development of mud crab farming. Disodium 5'-inosinate (I+G) and mannan oligosaccharides (MOS) are nationally approved feed additives that can improve the growth and immunity of farmed animals. However, they are not currently used in artificial pelleted feed for mud crabs, mainly because the appropriate dosage of these two substances in mud crab feed has not yet been determined. If the appropriate dosage of these two feed additives can be determined and applied to mud crab feed, it can effectively improve the growth and antioxidant capacity of mud crabs. Summary of the Invention

[0003] In view of the problems existing in the prior art, the purpose of this invention is to provide an application of 5'-flavor nucleotide disodium or mannan oligosaccharide as a feed additive.

[0004] A first aspect of the present invention provides the use of disodium 1,5'-flavor nucleotides or mannooligosaccharides as feed additives.

[0005] Furthermore, the feed additive is a feed additive specifically for mud crabs.

[0006] A second aspect of the invention provides the application of feed additives in improving the growth and antioxidant capacity of juvenile mud crabs.

[0007] Furthermore, the addition of disodium 5'-inosinolates and mannan oligosaccharides significantly improved the growth performance of juvenile mud crabs.

[0008] Furthermore, the addition of disodium 5'-inosinolates increased the weight gain rate of mud crabs by 1.5%-13%.

[0009] Furthermore, the addition of mannan oligosaccharides increased the weight gain rate of mud crabs by 5%-16%.

[0010] Furthermore, the antioxidant properties of juvenile mud crabs were significantly increased after the addition of disodium 5'-inosinolates and mannan oligosaccharides.

[0011] Furthermore, the addition of disodium 5'-inosinolates reduced the malondialdehyde content in mud crabs by 32%-50%.

[0012] Furthermore, the addition of mannan oligosaccharides reduced the malondialdehyde content in mud crabs by 30%-59%.

[0013] A third aspect of the present invention provides a feed specifically for mud crabs containing disodium 5'-inosinolates or mannooligosaccharides, comprising the following components in weight percentages: Fish meal 20-25%, casein 30-35%, gelatinized starch 10-13%, fish oil 2.5-3.5%, soybean oil 2.8-3.2%, phospholipids 1.5-2%, multivitamins 4-5%, minerals 3-4%, cholesterol 0-0.8%, dicalcium phosphate 1-1.5%, choline chloride 0-0.7%, sodium alginate 0-1%, taurine 0-0.5%, disodium 5'-ribonucleotide 0.5%-0.75% or mannan oligosaccharides 0%-0.8%, cellulose 10.55%-11.30%.

[0014] Furthermore, the amount of 5'-flavor nucleotide disodium added accounts for 0.45% of the total feed.

[0015] Furthermore, the amount of mannan oligosaccharide added accounts for 0.48-0.64% of the total feed.

[0016] Compared with the prior art, the present invention has the following beneficial effects: This invention uses disodium 5'-inosinolates or mannan oligosaccharide as an additive in the feed of mud crabs, which can significantly improve the weight gain rate of mud crabs and reduce the malondialdehyde content in the bodies of mud crabs. Detailed Implementation

[0017] The present invention will be further described below with reference to specific embodiments in order to better understand the technical solution.

[0018] Example 1

[0019] In this experiment, different disodium 5'-inosinate was added as an additive to the feed for *Scylla serrata* (a type of mud crab). The specific rearing method was as follows: During the experiment, the crabs were fed the experimental feed twice daily (9:30 AM and 5:30 PM), and the number of molting crabs and the number of deaths were recorded. One hour after feeding, 25% of the water was replaced, and any remaining feed, crab feces, shells, and carcasses were removed. During the experiment, the following environmental conditions were maintained: water temperature between 27°C and 31°C, salinity between 28 and 29 psu, dissolved oxygen greater than 6 mg / L, and ammonia nitrogen concentration less than 0.05 mg / L. Lights were turned on at 7:00 AM and off at 7:00 PM daily.

[0020] Table 1 shows the formulation details for each feed.

[0021] Table 1: Feed Formulation

[0022] The effects of different feed formulations on the growth indicators of mud crabs are shown in Table 2.

[0023] Table 2: Effects of different experimental diets on growth indicators of mud crab (Scylla serrata)

[0024] The effects of different feed formulations on the antioxidant and health indicators of mud crabs are shown in Table 3. Table 3: Effects of different experimental diets on antioxidant and health indicators of mud crab (Scylla serrata)

[0025] Growth Indicators: A 60-day culture experiment was conducted using five experimental diets with different contents of disodium 5'-inosinate (I+G). The results are shown in Table 2. The survival rate of *Scylla serrata* was not affected by the diet. The diet of group 3 (with 0.45% disodium 5'-inosinate (I+G) added) showed the highest final weight, weight gain rate, and specific growth rate, significantly higher than the other groups. The carapace length, carapace width, and body height of group 3 were also significantly higher than those of groups 2 and 5. The molting frequency of group 3 was significantly higher than that of group 1. In conclusion, adding 0.45% disodium 5'-inosinate (I+G) to the diet can significantly improve the growth of juvenile *Scylla serrata*.

[0026] Health Indicators: A 60-day aquaculture experiment was conducted using five experimental diets with different contents of disodium 5'-inosinate (I+G). The results are shown in Table 3. Malondialdehyde (MDA) is a product of lipid peroxidation, and its level directly reflects the degree of oxidative damage. Group 3 of the diet had the lowest MDA content, indicating that the *Scylla serrata* crabs in this group experienced the least oxidative damage. Simultaneously, this group also had the lowest superoxide dismutase (SOD) activity. SOD is a key enzyme for scavenging oxygen free radicals. Combined with the MDA indicator, Group 3 of the diet showed the least oxidative stress and was the healthiest. Alkaline phosphatase and acid phosphatase are indicators of liver function. The activities of these two enzymes were at low levels in Group 3 of the diet, indicating that the hepatopancreas of the *Scylla serrata* crabs was in a healthy state.

[0027] Example 2

[0028] Different types of mannan oligosaccharides were added as additives to the feed for *Scylla serrata* (a type of mud crab) during this experiment. The specific rearing method was as follows: During the experiment, the crabs were fed the experimental feed twice daily (9:30 AM and 5:30 PM), and the number of molting crabs and the number of dead crabs were recorded. One hour after feeding, 25% of the water was replaced, and any remaining feed, crab feces, shells, and carcasses were removed. The environmental conditions were controlled as follows during the experiment: water temperature was maintained between 27°C and 31°C, salinity between 28 and 29 psu, dissolved oxygen greater than 6 mg / L, and ammonia nitrogen concentration less than 0.05 mg / L. Lights were turned on at 7:00 AM and off at 7:00 PM daily.

[0029] The formulations of each feed are shown in Table 4.

[0030] Table 4: Feed Formulation

[0031] The effects of different feed formulations on the growth indicators of mud crabs are shown in Table 5.

[0032] Table 5: Effects of different feed formulations on growth indicators of mud crab (Scylla serrata)

[0033] The effects of different feed formulations on the antioxidant and health indicators of mud crabs are shown in Table 6. Table 6: Effects of different experimental diets on antioxidant and health indicators of mud crab (Scylla serrata)

[0034] Growth Indicators: An 8-week culture experiment was conducted using five experimental diets with different levels of mannooligosaccharide (MOS). The results are shown in Table 5. The survival rate and molting frequency of *Scylla serrata* were not affected by the diet. The diet of group 8 (with 0.48% MOS) showed the highest final weight, weight gain rate, and specific growth rate, significantly higher than that of groups 6, 7, and 10. The carapace length and width of group 8 were also significantly higher than those of groups 6 and 10. In conclusion, the addition of 0.48% MOS to the diet significantly improved the growth of juvenile *Scylla serrata*.

[0035] Health Indicators: An 8-week farming experiment was conducted using five experimental diets with different amounts of mannan oligosaccharides (MOS). The results are shown in Table 6. Malondialdehyde (MDA) is a product of lipid peroxidation, and its level directly reflects the degree of oxidative damage. Group 8 of the diet had the lowest MDA content, significantly lower than the other groups, indicating that the oxidative damage in this group of mud crabs was minimal. Simultaneously, this group had the highest superoxide dismutase (SOD) activity. SOD is a key enzyme for scavenging oxygen free radicals. Combined with the MDA indicator, the addition of 0.48% mannan oligosaccharides (MOS) to the diet significantly increased the antioxidant enzyme activity and reduced oxidative damage in mud crabs. Alkaline phosphatase and acid phosphatase are indicators of liver function. The activities of these two enzymes were at low levels in Group 8 of the diet, indicating that the hepatopancreas of the mud crabs was in a healthy state.

Claims

Application of 1,5'-flavor nucleotide disodium or mannan oligosaccharide as a feed additive.

2. The application as described in claim 1, characterized in that, The feed additive mentioned is a feed additive specifically for mud crabs.

3. The application as described in claim 1, characterized in that, Application of feed additives in improving the growth and antioxidant capacity of juvenile mud crabs.

4. A feed specifically for mud crabs containing disodium 5'-inosinolates or mannooligosaccharides, characterized in that, The components include the following percentages by mass: Fish meal 20-25%, casein 30-35%, gelatinized starch 10-13%, fish oil 2.5-3.5%, soybean oil 2.8-3.2%, phospholipids 1.5-2%, multivitamins 4-5%, minerals 3-4%, cholesterol 0-0.8%, dicalcium phosphate 1-1.5%, choline chloride 0-0.7%, sodium alginate 0-1%, taurine 0-0.5%, disodium 5'-ribonucleotide 0.5%-0.75% or mannan oligosaccharides 0%-0.8%, cellulose 10.55%-11.30%.

5. The feed for mud crabs containing disodium 5'-inosinolates or mannooligosaccharides as described in claim 4, characterized in that, The amount of 5'-flavor nucleotide disodium added accounts for 0.45% of the total feed.

6. The feed for mud crabs containing disodium 5'-inosinolates or mannooligosaccharides as described in claim 4, characterized in that, The amount of mannan oligosaccharide added accounts for 0.48-0.64% of the total feed.

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