Composite health-care beverage containing lucid ganoderma and preparation process of composite health-care beverage

Abstract

The invention relates to the technical field of health-care drinks, in particular to a composite health-care drink containing lucid ganoderma and a preparation process of the composite health-care drink. The invention relates to a preparation process of a composite health-care beverage containing lucid ganoderma. The preparation process comprises the following steps: preparing a lucid ganoderma extract, debitterizing the lucid ganoderma extract, preparing a poria cocos and rhizoma polygonati compound extraction concentrated solution, and preparing the composite health-care beverage. Ganoderma lucidum is used as a monarch, poria cocos is used as a minister, rhizoma polygonati is used as an assistant, prebiotics are matched, and the composition is suitable for people with low immunity and the like. The neodiosmin, the casein and the konjac powder are used for double bitterness inhibition, so that the mouth feel is improved; the ganoderma lucidum ingredients are efficiently extracted by a technology of first alcohol extraction and then water extraction; and through infrared and vacuum combined drying, the efficiency is improved, the component loss is reduced, and the health-care effect of the beverage is maximized.

Description

Technical Field

[0001] This invention relates to the field of health beverage technology, specifically to a compound health beverage containing Ganoderma lucidum and its preparation process. Background Technology

[0002] With the continuous improvement of national health awareness, the market demand for health drinks, as convenient and daily health consumer products, continues to grow. Consumers' demand for health drinks is shifting towards diversified directions such as "nutritional supplementation," "functional conditioning," and "natural safety," with a particularly significant increase in preference for compound drinks made from traditional medicinal and edible ingredients that combine taste and health benefits. Ganoderma lucidum, a traditional and precious medicinal and edible resource in my country, is recorded in the Compendium of Materia Medica as "benefiting the heart qi, calming the spirit, replenishing liver qi, and strengthening tendons and bones." Modern pharmacological research has further confirmed that the active ingredients in Ganoderma lucidum, such as polysaccharides, triterpenes, and amino acids, have multiple physiological functions, including enhancing immunity, regulating metabolism, and anti-oxidation, making it a high-quality raw material for developing health drinks.

[0003] However, the application of Ganoderma lucidum in the health beverage industry still faces many technical bottlenecks, restricting its industrialization and market acceptance: The active ingredients of Ganoderma lucidum are mostly encapsulated in the dense cell walls of the fungus. Traditional preparation processes (such as simple boiling, pulverizing, and soaking) are insufficient to effectively break down the cell wall structure, resulting in insufficient dissolution of active ingredients and a significant waste of these components. Furthermore, Ganoderma lucidum itself has a pronounced woody bitter taste, making single-ingredient or simply compounded Ganoderma lucidum drinks highly bitter and reducing consumer acceptance. While existing technologies mask the bitterness by adding sweeteners such as sucrose and fructose syrup, this often leads to excessively high sugar content, contradicting the trend towards low-sugar and sugar-free health products. Additionally, most Ganoderma lucidum health beverages currently on the market are simple mixtures of Ganoderma lucidum with single ingredients (such as red dates and goji berries), lacking a scientifically designed formulation based on the synergistic effects of these ingredients.

[0004] In summary, current Ganoderma lucidum health drinks on the market have significant technical deficiencies in terms of active ingredient utilization, taste and flavor, and formula synergy. Therefore, this application aims to develop a compound health drink containing Ganoderma lucidum that combines efficient extraction methods with both taste and health benefits, as well as its preparation process, in order to overcome the shortcomings of existing technologies and meet consumers' demand for high-quality, multifunctional Ganoderma lucidum health drinks. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a compound health drink containing Ganoderma lucidum and its preparation process.

[0006] This invention provides a preparation process for a compound health beverage containing Ganoderma lucidum, comprising: S1: Preparation of Ganoderma lucidum extract; First, infrared drying is used to dry the Ganoderma lucidum until the moisture content is 50-60%. Then, vacuum drying is used. The Ganoderma lucidum is dried by using both methods. After that, alcohol extraction is used to prepare alcohol extract and precipitate. The precipitate is then extracted with water to obtain water extract. The alcohol extract and water extract are concentrated separately and then mixed to obtain Ganoderma lucidum extract. S2: Debittering treatment of Ganoderma lucidum extract; Mixed solution A was prepared by mixing neodiamin solid powder, ethanol aqueous solution and Ganoderma lucidum extract as raw materials. Mixed solution B was prepared by adding casein, konjac flour and maltodextrin to deionized water. Mixed solution A and mixed solution B were mixed and subjected to colloid milling and homogenization to obtain Ganoderma lucidum complex. S3: Preparation of concentrated extract of Poria cocos and Polygonatum sibiricum; Poria cocos and Polygonatum sibiricum were subjected to dynamic circulating hot water extraction, followed by concentration, to obtain a concentrated extract of Poria cocos and Polygonatum sibiricum. S4: Preparation of compound health drinks; A soy protein isolate-nicotinamide mononucleotide complex was prepared using soy protein isolate and nicotinamide mononucleotide. Then, the soy protein isolate-nicotinamide mononucleotide complex, Ganoderma lucidum complex, Poria cocos and Polygonatum sibiricum extract concentrate and deionized water were mixed and then mixed with DHA oil. Sodium chloride was added for homogenization to obtain a complex liquid. Then, 50-60 parts by weight of the complex liquid, 5-10 parts by weight of prebiotics and 1-2 parts by weight of natural flavoring agent were mixed to prepare a compound health drink.

[0007] As a preferred aspect, S1: the preparation of Ganoderma lucidum extract specifically includes the following steps: S1.1: Place the cleaned Ganoderma lucidum in an infrared drying oven, set the drying temperature to 60-65℃, and the fixed radiation distance to 20-22cm, until the moisture content of the Ganoderma lucidum is 50-60%. Then place it in a vacuum drying oven, set the drying temperature to 60-65℃ and the vacuum degree to 0.1-0.2MPa, until the moisture content is less than 10%. Crush the dried Ganoderma lucidum and pass it through a 60-mesh sieve to obtain Ganoderma lucidum powder. S1.2: Add 40-50wt% ethanol aqueous solution to Ganoderma lucidum powder at a material-to-liquid ratio of 1:10-12, then adjust the pH to 5-7, add 2-3wt% cellulase, and extract by stirring in a water bath at 50-55℃ and 100-120r / min for 2-3h. Then centrifuge at 4000-5000r / min for 20-30min to obtain the ethanol extract and precipitate. S1.3: Add deionized water to the precipitate at a material-to-liquid ratio of 1:5-8, then homogenize at 8000-10000 r / min for 2-3 min, then extract at 90-95℃ under high temperature and high pressure for 20-30 min, and finally centrifuge at 8000-9000 r / min for 10-15 min to obtain the aqueous extract and precipitate residue; S1.4: The ethanol extract is concentrated at 60-62℃ and under a vacuum of -0.08MPa to recover ethanol, and concentrated to 1 / 10 of the original volume. The aqueous extract is concentrated at 60-62℃ and under a vacuum of -0.08MPa, and concentrated to 1 / 10 of the original volume. The concentrated ethanol extract and the concentrated aqueous extract are then mixed to obtain Ganoderma lucidum extract.

[0008] As a preferred aspect, S2: the debittering treatment of Ganoderma lucidum extract specifically includes the following steps: S2.1: Add 3-5 parts by weight of Xindiaomin solid powder to 10-12 parts by weight of 50wt% ethanol aqueous solution, stir and mix for 20-30 min to obtain a mixed system, then add Ganoderma lucidum extract to the mixed system, the Ganoderma lucidum extract accounting for 5-8wt% of the mixed system, stir and mix at 200-300 r / min for 20-30 min to obtain mixed solution A; S2.2: Add casein, konjac flour and maltodextrin to 100-120 parts by weight of deionized water, and then stir and mix at 300-350 r / min for 20-30 min to obtain mixed solution B; S2.3: Add mixed solution A to mixed solution B under stirring at 200-300 r / min, then stir and mix at 60-65℃ for 40-50 min. After mixing, pass the mixture through a colloid mill 3-5 times, then homogenize at 25-28 MPa pressure 2-3 times, and homogenize at 35-40 MPa pressure 1-2 times to obtain Ganoderma lucidum complex.

[0009] As a preferred aspect, the weight ratio of casein, konjac flour and maltodextrin in step S2.2 is 20-30:2-3:15-20.

[0010] As a preferred aspect, S3: the preparation of the concentrated extract of Poria cocos and Polygonatum sibiricum specifically includes the following steps: S3.1: Mix and grind dried and clean Poria cocos slices and dried and clean Polygonatum sibiricum slices that have been processed by nine steaming and nine sun-drying at a weight ratio of 5-6:3. Then add 12-14 times the total weight of the ground medicinal materials in deionized water and stir and soak at 200-300 r / min for 30-40 minutes to obtain the soaked medicinal materials. S3.2: The soaked medicinal materials are extracted using a dynamic circulating hot water extraction method at 90-95℃ for 2-3 hours. The extracted liquid is then filtered, centrifuged, and transferred to a vacuum concentration tank. The liquid is concentrated at 60-65℃ and -0.08MPa to 1 / 5 of its original volume to obtain a concentrated extract of Poria cocos and Polygonatum sibiricum.

[0011] As a preferred aspect, S4: the preparation of the compound health drink specifically includes the following steps: S4.1: Add soy protein isolate to 100 parts by weight of deionized water and stir for 20-30 min to prepare a 2.5-3 wt% soy protein isolate mixture. Then, under ice bath conditions, add 1.5-2 parts by weight of nicotinamide mononucleotide to the soy protein isolate mixture and stir magnetically for 2-4 h. Quick-freeze the mixed composite aqueous solution at -50--60℃ for 12-14 h, and then freeze-dry under vacuum for 24-25 h to obtain the soy protein isolate-nicotinamide mononucleotide complex. S4.2: Mix 2-3 parts by weight of soy protein isolate-nicotinamide mononucleotide complex, 5-8 parts by weight of Ganoderma lucidum complex, 3-5 parts by weight of Poria cocos and Polygonatum sibiricum compound extract concentrate and 50-60 parts by weight of deionized water to obtain an aqueous phase mixture. Add 10-12 parts by weight of DHA oil as the oil phase. Stir the oil phase slowly into the aqueous phase at 300-500 r / min for preliminary emulsification. Then add 0.01 mol / L sodium chloride and homogenize for 5-8 min to obtain a composite solution. S4.3: Mix 50-60 parts by weight of compound liquid, 5-10 parts by weight of prebiotics, and 1-2 parts by weight of natural flavoring agent, ultrasonically mix for 20-30 minutes, then homogenize 2-3 times at 10-15 MPa using a high-pressure homogenizer, and finally sterilize to obtain a compound health drink.

[0012] As a preferred aspect, the temperature of the dynamic circulating hot water extraction in step S3.2 is 90-95℃, and the time is 2-3h.

[0013] As a preferred aspect, the natural flavoring agent in step S4.3 is composed of mogroside and ammonium glycyrrhizate in a mass ratio of 1-2:1.

[0014] As a preferred aspect, the prebiotic in step S4.3 is composed of fructooligosaccharides and inulin in a mass ratio of 1-2:1.

[0015] The present invention also provides a compound health drink containing Ganoderma lucidum, which is prepared by the preparation process of any one of the compound health drinks containing Ganoderma lucidum described in the present invention.

[0016] The present invention has the following advantages: 1. This invention uses Ganoderma lucidum as the core ingredient, which plays a leading role in immune regulation and antioxidant functions. Poria cocos is used as an assistant ingredient; it promotes diuresis and eliminates dampness, strengthens the spleen and calms the mind, thus assisting Ganoderma lucidum in enhancing its spleen-strengthening effect. Furthermore, the abundant polysaccharides in Poria cocos synergistically enhance the immune-boosting effect with those of Ganoderma lucidum. Polygonatum sibiricum, which tonifies qi and nourishes yin, strengthens the spleen and moistens the lungs, serves as an adjuvant ingredient, both assisting the tonifying effects of Ganoderma lucidum and Poria cocos and mitigating the bitterness of Ganoderma lucidum with its sweetness. Simultaneously, the polysaccharides of Polygonatum sibiricum and prebiotics together form a gut health regulating combination. The interaction between the components and their synergy with prebiotics significantly improve the efficacy of this compound health drink. It is suitable for daily consumption by people with low immunity, those in a sub-healthy state, those with poor spleen and stomach function, and those concerned about gut health, to improve bodily functions and enhance physical fitness.

[0017] 2. This invention uses a mixture of neodiamine, casein, and konjac flour to process Ganoderma lucidum extract. Neodiamine molecules contain multiple polar groups such as hydroxyl and ester groups, which can form non-covalent bonds such as hydrogen bonds and hydrophobic interactions with the hydrophobic groups of triterpenoid components, forming a "neodiamine-triterpenoid" complex molecule. This combination alters the molecular conformation of the triterpenoid components, preventing them from effectively binding to oral bitter taste receptors, thereby directly reducing the intensity of bitter taste perception. Simultaneously, the dissolved konjac flour forms a high-viscosity sol, which can intertwine with casein microspheres to construct a denser colloidal network that can encapsulate the "neodiamine-triterpenoid" complex molecule, preventing it from directly contacting the bitter taste receptors on the oral mucosa, achieving "physical shielding" and further reducing bitterness. By encapsulating neodiamine molecules, a dual bitter-suppressing effect of targeted binding and physical encapsulation is achieved, effectively improving the taste of the compound health drink.

[0018] 3. This invention uses soy protein isolate-nicotinamide mononucleotide complex as a stabilizer and DHA oil as the oil phase. The soy protein isolate-nicotinamide mononucleotide complex can form a unique three-dimensional network structure in the continuous phase, thereby encapsulating and loading the active ingredients of traditional Chinese medicine. Through the controlled release effect of the network structure, the active ingredients are prevented from being rapidly decomposed by gastric acid in the stomach, allowing them to reach the intestines smoothly and be released slowly, prolonging the action time of the ingredients in the intestines and creating sufficient conditions for absorption. In addition, sodium chloride is added, which improves the stability of the compound solution, avoids premature leakage of active ingredients due to emulsion stratification and aggregation, and can significantly improve the sustained release effect of active ingredients, thereby facilitating the retention of active ingredients in the intestines and effectively improving their utilization.

[0019] 4. This invention employs a combined infrared drying and vacuum drying method to dry Ganoderma lucidum. The advantages of vacuum drying and infrared drying complement each other. First, infrared drying uses infrared rays to directly act on the water molecules inside the Ganoderma lucidum, causing the water molecules to absorb energy and vibrate rapidly, escaping. The "internal heating" characteristic of infrared drying significantly reduces resistance to water migration, greatly increasing the dehydration speed and quickly completing the "mass dehydration stage." When the moisture content of the Ganoderma lucidum decreases significantly, the water is in the form of bound water. On the one hand, the vacuum condition lowers the boiling point of water, making the bound water easier to vaporize; on the other hand, the vacuum... An empty environment can quickly remove vaporized moisture, preventing water vapor from condensing on the surface of Ganoderma lucidum and causing "secondary water absorption". The combination of the two methods can achieve a step-by-step dehydration of "first quickly removing free water, then deeply removing bound water", which can significantly improve drying efficiency, shorten the total drying cycle, and greatly improve industrial production efficiency. Furthermore, the combined use of infrared drying and vacuum drying to dry Ganoderma lucidum reduces the loss of sugar substances due to the milder heating conditions and the negative pressure environment that isolates air. It can also significantly improve the retention rate of Ganoderma lucidum polysaccharides and increase the content of Ganoderma lucidum polysaccharides in subsequent Ganoderma lucidum extracts.

[0020] 5. This invention employs a targeted separation technology of "alcohol extraction followed by water extraction." Alcohol extraction first efficiently and specifically extracts fat-soluble active ingredients; then, water extraction is performed using the defatted precipitate, resulting in a more porous medicinal material structure, which greatly improves extraction efficiency and polysaccharide yield. This targeted separation technology can selectively and efficiently extract effective components of different polarities from Ganoderma lucidum, avoiding component loss or damage caused by traditional single extraction methods. It maximizes the retention of the full spectrum of active substances, achieving "comprehensive extraction while considering both primary and secondary components." The retention of highly active ingredients maximizes the efficacy of the compound health drink. Attached Figure Description

[0021] Figure 1 This is a flowchart illustrating the preparation process of a compound health drink containing Ganoderma lucidum used in an embodiment of the present invention. Detailed Implementation

[0022] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of this invention.

[0023] Example 1: A preparation process for a compound health drink containing Ganoderma lucidum, referring to... Figure 1 ,include: S1: Preparation of Ganoderma lucidum extract S1.1: Place the cleaned Ganoderma lucidum in an infrared drying oven, set the drying temperature to 60℃, and the fixed radiation distance to 20cm. Dry until the moisture content of the Ganoderma lucidum is 50%. Then place it in a vacuum drying oven, set the drying temperature to 60℃ and the vacuum degree to 0.1MPa. Dry until the moisture content is less than 10%. Crush the dried Ganoderma lucidum and pass it through a 60-mesh sieve to obtain Ganoderma lucidum powder. S1.2: Add 40wt% ethanol aqueous solution to Ganoderma lucidum powder at a material-to-liquid ratio of 1:10, then adjust the pH to 5, add 2wt% cellulase, and extract by stirring in a water bath at 50℃ and 100r / min for 2h. Then centrifuge at 4000r / min for 20min to obtain ethanol extract and precipitate. S1.3: Add deionized water to the precipitate at a material-to-liquid ratio of 1:5, then homogenize at 8000 r / min for 2 min, then extract at 90℃ under high temperature and high pressure for 20 min, and finally centrifuge at 8000 r / min for 10 min to obtain the aqueous extract and precipitate residue. S1.4: The ethanol extract was concentrated at 60℃ and under vacuum of -0.08MPa to recover ethanol and was concentrated to 1 / 10 of the original volume. The aqueous extract was concentrated at 60℃ and under vacuum of -0.08MPa to 1 / 10 of the original volume. The concentrated ethanol extract and the concentrated aqueous extract were then mixed to obtain Ganoderma lucidum extract. S2: Debittering treatment of Ganoderma lucidum extract S2.1: Add 3 parts by weight of neodiamin solid powder to 10 parts by weight of 50 wt% ethanol aqueous solution, stir and mix for 20 min to obtain a mixed system, then add Ganoderma lucidum extract to the mixed system, the Ganoderma lucidum extract accounts for 5 wt% of the mixed system, stir and mix at 200 r / min for 20 min to obtain mixed solution A; S2.2: Add 20 parts by weight of casein, 2 parts by weight of konjac flour and 15 parts by weight of maltodextrin to 100 parts by weight of deionized water, and then stir and mix at 300 r / min for 20 min to obtain mixed solution B. S2.3: Add mixed solution A to mixed solution B under stirring at 200 r / min, then stir and mix at 60℃ for 40 min. After mixing, the mixture is ground three times by a colloid mill, then homogenized twice at 25 MPa pressure, and once at 35 MPa pressure to obtain Ganoderma lucidum complex. S3: Preparation of concentrated extract of Poria cocos and Polygonatum sibiricum S3.1: Mix and grind dried and clean Poria cocos slices and dried and clean Polygonatum sibiricum slices that have been processed by nine steaming and nine sun-drying in a weight ratio of 5:3. Then add 12 times the total weight of the ground medicinal materials in deionized water and stir and soak at 200 r / min for 30 min to obtain the soaked medicinal materials. S3.2: The soaked medicinal materials were extracted using a dynamic circulating hot water extraction method at 90℃ for 2 hours. The extracted liquid was then filtered, centrifuged, and transferred to a vacuum concentration tank. The liquid was concentrated at 60℃ and -0.08MPa to 1 / 5 of its original volume to obtain a concentrated extract of Poria cocos and Polygonatum sibiricum. S4: Preparation of Compound Health Drinks S4.1: Soy protein isolate was added to 100 parts by weight of deionized water and stirred for 20 min to prepare a 2.5 wt% soy protein isolate mixture. Then, 1.5 parts by weight of nicotinamide mononucleotide was added to the soy protein isolate mixture under ice bath conditions and magnetically stirred for 2 h. The mixed composite aqueous solution was then rapidly frozen at -50°C for 12 h and then freeze-dried under vacuum for 24 h to obtain the soy protein isolate-nicotinamide mononucleotide complex. S4.2: Mix 2 parts by weight of soy protein isolate-nicotinamide mononucleotide complex, 5 parts by weight of Ganoderma lucidum complex, 3 parts by weight of Poria cocos and Polygonatum sibiricum compound extract concentrate and 50 parts by weight of deionized water to obtain an aqueous phase mixture. Add 10 parts by weight of DHA oil as the oil phase. Stir at 300 r / min and slowly add the oil phase to the aqueous phase for preliminary emulsification. Then add 0.01 mol / L sodium chloride and homogenize for 5 min to obtain a composite solution. S4.3: Mix 50 parts by weight of compound liquid, 5 parts by weight of prebiotics and 1 part by weight of natural flavoring agent, ultrasonically mix for 20 minutes, then homogenize twice at 10 MPa using a high-pressure homogenizer, and finally sterilize to obtain a compound health drink. The natural flavoring agent is composed of mogroside and ammonium glycyrrhizate in a 1:1 mass ratio; the prebiotic is composed of fructooligosaccharides and inulin in a 1:1 mass ratio.

[0024] Example 2: Preparation process of a compound health drink containing Ganoderma lucidum (see [reference]). Figure 1 ,include: S1: Preparation of Ganoderma lucidum extract S1.1: Place the cleaned Ganoderma lucidum in an infrared drying oven, set the drying temperature to 65℃, and the fixed radiation distance to 22cm. Dry until the moisture content of the Ganoderma lucidum is 50%. Then place it in a vacuum drying oven, set the drying temperature to 65℃ and the vacuum degree to 0.2MPa. Dry until the moisture content is less than 10%. Crush the dried Ganoderma lucidum and pass it through a 60-mesh sieve to obtain Ganoderma lucidum powder. S1.2: Add 40wt% ethanol aqueous solution to Ganoderma lucidum powder at a material-to-liquid ratio of 1:10, then adjust the pH to 5, add 2wt% cellulase, and extract by stirring in a water bath at 55℃ and 120r / min for 3h. Then centrifuge at 5000r / min for 30min to obtain ethanol extract and precipitate. S1.3: Add deionized water to the precipitate at a material-to-liquid ratio of 1:5, then homogenize at 10000 r / min for 3 min, then extract at 95℃ under high temperature and high pressure for 30 min, and finally centrifuge at 9000 r / min for 15 min to obtain the aqueous extract and precipitate residue. S1.4: The ethanol extract was concentrated at 62℃ and under vacuum of -0.08MPa to recover ethanol and was concentrated to 1 / 10 of the original volume. The aqueous extract was concentrated at 62℃ and under vacuum of -0.08MPa to 1 / 10 of the original volume. The concentrated ethanol extract and the concentrated aqueous extract were then mixed to obtain Ganoderma lucidum extract. S2: Debittering treatment of Ganoderma lucidum extract S2.1: Add 3 parts by weight of neodiamin solid powder to 10 parts by weight of 50 wt% ethanol aqueous solution, stir and mix for 30 min to obtain a mixed system, then add Ganoderma lucidum extract to the mixed system, the Ganoderma lucidum extract accounts for 5 wt% of the mixed system, stir and mix at 300 r / min for 30 min to obtain mixed solution A; S2.2: Add 20 parts by weight of casein, 2 parts by weight of konjac flour and 15 parts by weight of maltodextrin to 100 parts by weight of deionized water, and then stir and mix at 350 r / min for 30 min to obtain mixed solution B. S2.3: Add mixed solution A to mixed solution B under stirring at 300 r / min, then stir and mix at 65℃ for 50 min. After mixing, the mixture is ground 5 times by a colloid mill, then homogenized 3 times at 28 MPa pressure and 2 times at 40 MPa pressure to obtain Ganoderma lucidum complex. S3: Preparation of concentrated extract of Poria cocos and Polygonatum sibiricum S3.1: Mix and grind dried and clean Poria cocos slices and dried and clean Polygonatum sibiricum slices that have been processed by nine steaming and nine sun-drying in a weight ratio of 5:3. Then add 12 times the total weight of the ground medicinal materials in deionized water and stir and soak at 300 r / min for 40 min to obtain the soaked medicinal materials. S3.2: The soaked medicinal materials were extracted using a dynamic circulating hot water extraction method at 95℃ for 3 hours. The extracted liquid was then filtered, centrifuged, and transferred to a vacuum concentration tank. The liquid was concentrated at 65℃ and -0.08MPa to 1 / 5 of its original volume to obtain a concentrated extract of Poria cocos and Polygonatum sibiricum. S4: Preparation of Compound Health Drinks S4.1: Soy protein isolate was added to 100 parts by weight of deionized water and stirred for 30 min to prepare a 2.5 wt% soy protein isolate mixture. Then, 1.5 parts by weight of nicotinamide mononucleotide was added to the soy protein isolate mixture under ice bath conditions and magnetically stirred for 4 h. The mixed composite aqueous solution was then rapidly frozen at -60°C for 14 h and then freeze-dried under vacuum for 25 h to obtain the soy protein isolate-nicotinamide mononucleotide complex. S4.2: Mix 2 parts by weight of soy protein isolate-nicotinamide mononucleotide complex, 5 parts by weight of Ganoderma lucidum complex, 3 parts by weight of Poria cocos and Polygonatum sibiricum compound extract concentrate and 50 parts by weight of deionized water to obtain an aqueous phase mixture. Add 10 parts by weight of DHA oil as the oil phase. Stir at 500 r / min and slowly add the oil phase to the aqueous phase for preliminary emulsification. Then add 0.01 mol / L sodium chloride and homogenize for 8 min to obtain a composite solution. S4.3: Mix 50 parts by weight of compound liquid, 5 parts by weight of prebiotics and 1 part by weight of natural flavoring agent, sonicate for 30 minutes, then homogenize three times at 15 MPa using a high-pressure homogenizer, and finally sterilize to obtain a compound health drink. The natural flavoring agent is composed of mogroside and ammonium glycyrrhizate in a 1:1 mass ratio; the prebiotic is composed of fructooligosaccharides and inulin in a 1:1 mass ratio.

[0025] Example 3: Preparation process of a compound health drink containing Ganoderma lucidum (see [reference]). Figure 1 ,include: S1: Preparation of Ganoderma lucidum extract S1.1: Place the cleaned Ganoderma lucidum in an infrared drying oven, set the drying temperature to 60℃, and the fixed radiation distance to 20cm. Dry until the moisture content of the Ganoderma lucidum is 60%. Then place it in a vacuum drying oven, set the drying temperature to 60℃ and the vacuum degree to 0.1MPa. Dry until the moisture content is less than 10%. Crush the dried Ganoderma lucidum and pass it through a 60-mesh sieve to obtain Ganoderma lucidum powder. S1.2: Add 50wt% ethanol aqueous solution to Ganoderma lucidum powder at a material-to-liquid ratio of 1:12, then adjust the pH to 7, add 3wt% cellulase, and extract by stirring in a water bath at 50℃ and 100r / min for 2h. Then centrifuge at 4000r / min for 20min to obtain ethanol extract and precipitate. S1.3: Add deionized water to the precipitate at a material-to-liquid ratio of 1:8, then homogenize at 8000 r / min for 2 min, then extract at 90℃ under high temperature and high pressure for 20 min, and finally centrifuge at 8000 r / min for 10 min to obtain the aqueous extract and precipitate residue. S1.4: The ethanol extract was concentrated at 60℃ and under vacuum of -0.08MPa to recover ethanol and was concentrated to 1 / 10 of the original volume. The aqueous extract was concentrated at 60℃ and under vacuum of -0.08MPa to 1 / 10 of the original volume. The concentrated ethanol extract and the concentrated aqueous extract were then mixed to obtain Ganoderma lucidum extract. S2: Debittering treatment of Ganoderma lucidum extract S2.1: Add 5 parts by weight of neodiamin solid powder to 12 parts by weight of 50 wt% ethanol aqueous solution, stir and mix for 20 min to obtain a mixed system, then add Ganoderma lucidum extract to the mixed system, the Ganoderma lucidum extract accounts for 8 wt% of the mixed system, stir and mix at 200 r / min for 20 min to obtain mixed solution A; S2.2: Add 30 parts by weight of casein, 3 parts by weight of konjac flour and 20 parts by weight of maltodextrin to 120 parts by weight of deionized water, and then stir and mix at 300 r / min for 20 min to obtain mixed solution B; S2.3: Add mixed solution A to mixed solution B under stirring at 200 r / min, then stir and mix at 60℃ for 40 min. After mixing, the mixture is ground three times by a colloid mill, then homogenized twice at 25 MPa pressure, and once at 35 MPa pressure to obtain Ganoderma lucidum complex. S3: Preparation of concentrated extract of Poria cocos and Polygonatum sibiricum S3.1: Mix and grind dried and clean Poria cocos slices and dried and clean Polygonatum sibiricum slices that have been processed by nine steaming and nine sun-drying in a weight ratio of 6:3. Then add 14 times the total weight of the ground medicinal materials in deionized water and stir and soak at 200 r / min for 30 min to obtain the soaked medicinal materials. S3.2: The soaked medicinal materials were extracted using a dynamic circulating hot water extraction method at 90℃ for 2 hours. The extracted liquid was then filtered, centrifuged, and transferred to a vacuum concentration tank. The liquid was concentrated at 60℃ and -0.08MPa to 1 / 5 of its original volume to obtain a concentrated extract of Poria cocos and Polygonatum sibiricum. S4: Preparation of Compound Health Drinks S4.1: Soy protein isolate was added to 100 parts by weight of deionized water and stirred for 20 min to prepare a 2.5 wt% soy protein isolate mixture. Then, 1.5 parts by weight of nicotinamide mononucleotide was added to the soy protein isolate mixture under ice bath conditions and magnetically stirred for 4 h. The mixed composite aqueous solution was then rapidly frozen at -50°C for 12 h and then freeze-dried under vacuum for 24 h to obtain the soy protein isolate-nicotinamide mononucleotide complex. S4.2: Mix 3 parts by weight of soy protein isolate-nicotinamide mononucleotide complex, 8 parts by weight of Ganoderma lucidum complex, 5 parts by weight of Poria cocos and Polygonatum sibiricum compound extract concentrate and 60 parts by weight of deionized water to obtain an aqueous phase mixture. Use 12 parts by weight of DHA oil as the oil phase. Stir at 300 r / min and slowly add the oil phase to the aqueous phase for preliminary emulsification. Then add 0.01 mol / L sodium chloride and homogenize for 5 min to obtain a composite solution. S4.3: Mix 60 parts by weight of compound liquid, 10 parts by weight of prebiotics and 2 parts by weight of natural flavoring agent, sonicate for 20 minutes, then homogenize twice at 10 MPa using a high-pressure homogenizer, and finally sterilize to obtain compound health drink. The natural flavoring agent is composed of mogroside and ammonium glycyrrhizate in a mass ratio of 2:1; the prebiotic is composed of fructooligosaccharides and inulin in a mass ratio of 2:1.

[0026] Comparative Example 1 differs from Example 1 in that only infrared drying is used in step S1.1 to dry the Ganoderma lucidum until the moisture content is less than 10%, while the other steps remain unchanged in preparing the compound health drink. This is referred to as Comparative Example 1.

[0027] Comparative Example 2 differs from Example 1 in that only vacuum drying is used in step S1.1 to dry the product to a moisture content of less than 10%, while the other steps remain unchanged in the preparation of the compound health drink. This is referred to as Comparative Example 2.

[0028] Comparative Example 3 differs from Example 1 in that, in step S1.1, vacuum drying is first used to dry the Ganoderma lucidum until the moisture content is 50%, and then infrared drying is used to dry it until the moisture content is less than 10%. The remaining steps are unchanged to prepare the compound health drink, which is referred to as Comparative Example 3.

[0029] Comparative Example 4 differs from Example 1 in that it uses only the alcohol extraction method in S1.2 to extract Ganoderma lucidum powder twice, combines the two alcohol extracts, and concentrates them under vacuum to 1 / 10 of the original volume to obtain Ganoderma lucidum extract. The remaining steps are unchanged to prepare a compound health drink, which is referred to as Comparative Example 4.

[0030] Comparative Example 5 differs from Example 1 in that it uses the water extraction method in S1.3 to extract Ganoderma lucidum powder twice, combines the two water extracts, and concentrates them under vacuum to 1 / 10 of the original volume to obtain Ganoderma lucidum extract. The remaining steps are unchanged to prepare a compound health drink, and it is referred to as Comparative Example 5.

[0031] Comparative Example 6 differs from Example 1 in that it first uses the water extraction method in S1.3 to extract Ganoderma lucidum powder, obtaining an aqueous extract and a precipitate, and then uses the alcohol extraction method in S1.2 to extract the precipitate, obtaining an alcohol extract and a precipitate residue. The remaining steps remain unchanged in preparing the compound health drink, and it is referred to as Comparative Example 6.

[0032] Comparative Example 7 differs from Example 1 in that step S2 is removed, and the Ganoderma lucidum complex in S4.2 is replaced with Ganoderma lucidum extract, while the remaining steps remain unchanged to prepare the compound health drink. This is referred to as Comparative Example 7.

[0033] Comparative Example 8 differs from Example 1 in that step S2 is removed, and the Ganoderma lucidum complex in S4.2 is replaced with Ganoderma lucidum extract and neodiamine, while the remaining steps remain unchanged to prepare the compound health drink. This is referred to as Comparative Example 8.

[0034] Comparative Example 9 differs from Example 1 in that it removes neodiamine from step S2.1 while keeping the other steps unchanged in preparing the compound health drink.

[0035] Comparative Example 10 differs from Example 1 in that sodium chloride in step S4.2 is removed, while the remaining steps remain unchanged in the preparation of the compound health drink. It is referred to as Comparative Example 10.

[0036] The content of Ganoderma lucidum polysaccharides in the extracts prepared in Examples 1-3 and Comparative Examples 1-3 was determined by ultraviolet-visible spectrophotometry, and the drying time was also measured. The average value of three measurements was obtained and the results are shown in Table 1.

[0037] Table 1. Results of Ganoderma lucidum polysaccharide content and drying time in Examples 1-3 and Comparative Examples 1-3

[0038] As can be seen from the data in Table 1, the present invention uses a combination of infrared drying and vacuum drying to dry Ganoderma lucidum. Compared with vacuum drying or infrared drying alone, this method can significantly improve the retention rate of Ganoderma lucidum polysaccharides and increase the content of Ganoderma lucidum polysaccharides in the subsequent Ganoderma lucidum extract.

[0039] Furthermore, the amount of Ganoderma lucidum polysaccharides in Comparative Example 3 was also lower than that in the Example, indicating that the method of using a combination of infrared drying and vacuum drying is more effective in increasing the content of Ganoderma lucidum polysaccharides than vacuum drying followed by infrared drying.

[0040] As can be seen from the drying time, the method of combining infrared drying and vacuum drying can significantly improve drying efficiency, shorten the total drying cycle, and greatly improve the efficiency of industrial production compared to vacuum drying and vacuum drying followed by infrared drying.

[0041] The contents of Ganoderma lucidum polysaccharides and Ganoderma lucidum triterpenes in the extracts prepared in Examples 1-3 and Comparative Examples 4-6 were determined by ultraviolet-visible spectrophotometry. The average value was taken from three measurements, and the results are shown in Table 2.

[0042] Table 2. Results of determination of Ganoderma lucidum polysaccharide content and Ganoderma lucidum triterpenoid content in Examples 1-3 and Comparative Examples 4-6

[0043] As can be seen from the data in Table 2, compared with the traditional process of "water extraction followed by alcohol extraction" or single solvent extraction, the directional separation technology of "alcohol extraction followed by water extraction" used in this invention can significantly increase the content of active ingredients in the extract.

[0044] Sensory evaluations were performed on the compound health drinks prepared in Examples 1-3 and Comparative Examples 7-9. The evaluation results are shown in Table 3.

[0045] The sensory evaluation of the compound health drink was conducted by 30 adults with normal taste. The bitterness value was evaluated by "+", with more "+" indicating more bitterness. The average value was taken.

[0046] "+": No bitterness or very mild bitterness; "++": Distinct bitterness; "+++": Strong bitterness; "++++": Extremely bitterness.

[0047] Table 3. Bitterness value evaluation results of Examples 1-3 and Comparative Examples 7-9

[0048] As can be seen from the data in Table 3, the use of a mixture of neodiazepines, casein, and konjac flour to process Ganoderma lucidum extract effectively reduced the bitterness of the compound health drink and improved its taste through targeted binding and physical encapsulation.

[0049] The sustained-release properties of Ganoderma lucidum polysaccharides in the composite solutions prepared in Examples 1-3 and Comparative Example 10 were determined by dynamic dialysis. The determination was performed three times and the average value was taken. The results are shown in Table 4.

[0050] Table 4. Results of sustained-release performance tests for Examples 1-3 and Comparative Example 10

[0051] As can be seen from the data in Table 4, the release amount of Comparative Example 10 is better than that of the Example within the same time period, indicating that the addition of sodium chloride can significantly improve the sustained-release effect of the active ingredient, thereby effectively improving its utilization. As can be seen from the data of Examples 1-3, the composite liquid prepared by the present invention using soybean protein isolate-nicotinamide mononucleotide complex as stabilizer and DHA oil as oil phase can improve the sustained-release effect of the active ingredient, allowing it to be released slowly.

[0052] It should be understood that those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims. Parts not described in detail in this specification are prior art known to those skilled in the art.

Claims

1. A preparation process for a compound health beverage containing Ganoderma lucidum, characterized in that, include: S1: Preparation of Ganoderma lucidum extract; First, infrared drying is used to dry the Ganoderma lucidum until the moisture content is 50-60%. Then, vacuum drying is used. The Ganoderma lucidum is dried by using both methods. After that, alcohol extraction is used to prepare alcohol extract and precipitate. The precipitate is then extracted with water to obtain water extract. The alcohol extract and water extract are concentrated separately and then mixed to obtain Ganoderma lucidum extract. S2: Debittering treatment of Ganoderma lucidum extract; Mixed solution A was prepared by mixing neodiamin solid powder, ethanol aqueous solution and Ganoderma lucidum extract as raw materials. Mixed solution B was prepared by adding casein, konjac flour and maltodextrin to deionized water. Mixed solution A and mixed solution B were mixed and subjected to colloid milling and homogenization to obtain Ganoderma lucidum complex. S3: Preparation of concentrated extract of Poria cocos and Polygonatum sibiricum; Poria cocos and Polygonatum sibiricum were subjected to dynamic circulating hot water extraction, followed by concentration, to obtain a concentrated extract of Poria cocos and Polygonatum sibiricum. S4: Preparation of compound health drinks; A soy protein isolate-nicotinamide mononucleotide complex was prepared using soy protein isolate and nicotinamide mononucleotide. Then, the soy protein isolate-nicotinamide mononucleotide complex, Ganoderma lucidum complex, Poria cocos and Polygonatum sibiricum extract concentrate and deionized water were mixed and then mixed with DHA oil. Sodium chloride was added for homogenization to obtain a complex liquid. Then, 50-60 parts by weight of the complex liquid, 5-10 parts by weight of prebiotics and 1-2 parts by weight of natural flavoring agent were mixed to prepare a compound health drink.

2. The preparation process of a compound health drink containing Ganoderma lucidum according to claim 1, characterized in that, S1: The preparation of Ganoderma lucidum extract includes the following steps: S1.1: Place the cleaned Ganoderma lucidum in an infrared drying oven, set the drying temperature to 60-65℃, and the fixed radiation distance to 20-22cm, until the moisture content of the Ganoderma lucidum is 50-60%. Then place it in a vacuum drying oven, set the drying temperature to 60-65℃ and the vacuum degree to 0.1-0.2MPa, until the moisture content is less than 10%. Crush the dried Ganoderma lucidum and pass it through a 60-mesh sieve to obtain Ganoderma lucidum powder. S1.2: Add 40-50wt% ethanol aqueous solution to Ganoderma lucidum powder at a material-to-liquid ratio of 1:10-12, then adjust the pH to 5-7, add 2-3wt% cellulase, and extract by stirring in a water bath at 50-55℃ and 100-120r / min for 2-3h. Then centrifuge at 4000-5000r / min for 20-30min to obtain the ethanol extract and precipitate. S1.3: Add deionized water to the precipitate at a material-to-liquid ratio of 1:5-8, then homogenize at 8000-10000 r / min for 2-3 min, then extract at 90-95℃ under high temperature and high pressure for 20-30 min, and finally centrifuge at 8000-9000 r / min for 10-15 min to obtain the aqueous extract and precipitate residue; S1.4: The ethanol extract is concentrated at 60-62℃ and under a vacuum of -0.08MPa to recover ethanol, and concentrated to 1 / 10 of the original volume. The aqueous extract is concentrated at 60-62℃ and under a vacuum of -0.08MPa, and concentrated to 1 / 10 of the original volume. The concentrated ethanol extract and the concentrated aqueous extract are then mixed to obtain Ganoderma lucidum extract.

3. The preparation process of a compound health beverage containing Ganoderma lucidum according to claim 1, characterized in that, S2: Debittering treatment of Ganoderma lucidum extract, specifically including the following steps: S2.1: Add 3-5 parts by weight of Xindiaomin solid powder to 10-12 parts by weight of 50wt% ethanol aqueous solution, stir and mix for 20-30 min to obtain a mixed system, then add Ganoderma lucidum extract to the mixed system, the Ganoderma lucidum extract accounting for 5-8wt% of the mixed system, stir and mix at 200-300 r / min for 20-30 min to obtain mixed solution A; S2.2: Add casein, konjac flour and maltodextrin to 100-120 parts by weight of deionized water, and then stir and mix at 300-350 r / min for 20-30 min to obtain mixed solution B; S2.3: Add mixed solution A to mixed solution B under stirring at 200-300 r / min, then stir and mix at 60-65℃ for 40-50 min. After mixing, pass the mixture through a colloid mill 3-5 times, then homogenize at 25-28 MPa pressure 2-3 times, and homogenize at 35-40 MPa pressure 1-2 times to obtain Ganoderma lucidum complex.

4. The preparation process of a compound health drink containing Ganoderma lucidum according to claim 3, characterized in that, In step S2.2, the weight ratio of casein, konjac flour and maltodextrin is 20-30:2-3:15-20.

5. The preparation process of a compound health drink containing Ganoderma lucidum according to claim 1, characterized in that, S3: The preparation of the concentrated extract of Poria cocos and Polygonatum sibiricum compound includes the following steps: S3.1: Mix and grind dried and clean Poria cocos slices and dried and clean Polygonatum sibiricum slices that have been processed by nine steaming and nine sun-drying at a weight ratio of 5-6:

3. Then add 12-14 times the total weight of the ground medicinal materials in deionized water and stir and soak at 200-300 r / min for 30-40 minutes to obtain the soaked medicinal materials. S3.2: The soaked medicinal materials are extracted using a dynamic circulating hot water extraction method at 90-95℃ for 2-3 hours. The extracted liquid is then filtered, centrifuged, and transferred to a vacuum concentration tank. The liquid is concentrated at 60-65℃ and -0.08MPa to 1 / 5 of its original volume to obtain a concentrated extract of Poria cocos and Polygonatum sibiricum.

6. The preparation process of a compound health drink containing Ganoderma lucidum according to claim 1, characterized in that, S4: The preparation of compound health drinks includes the following steps: S4.1: Add soy protein isolate to 100 parts by weight of deionized water and stir for 20-30 min to prepare a 2.5-3 wt% soy protein isolate mixture. Then, under ice bath conditions, add 1.5-2 parts by weight of nicotinamide mononucleotide to the soy protein isolate mixture and stir magnetically for 2-4 h. Quick-freeze the mixed composite aqueous solution at -50--60℃ for 12-14 h, and then freeze-dry under vacuum for 24-25 h to obtain the soy protein isolate-nicotinamide mononucleotide complex. S4.2: Mix 2-3 parts by weight of soy protein isolate-nicotinamide mononucleotide complex, 5-8 parts by weight of Ganoderma lucidum complex, 3-5 parts by weight of Poria cocos and Polygonatum sibiricum compound extract concentrate and 50-60 parts by weight of deionized water to obtain an aqueous phase mixture. Add 10-12 parts by weight of DHA oil as the oil phase. Stir the oil phase slowly into the aqueous phase at 300-500 r / min for preliminary emulsification. Then add 0.01 mol / L sodium chloride and homogenize for 5-8 min to obtain a composite solution. S4.3: Mix 50-60 parts by weight of compound liquid, 5-10 parts by weight of prebiotics, and 1-2 parts by weight of natural flavoring agent, ultrasonically mix for 20-30 minutes, then homogenize 2-3 times at 10-15 MPa using a high-pressure homogenizer, and finally sterilize to obtain a compound health drink.

7. The preparation process of a compound health drink containing Ganoderma lucidum according to claim 5, characterized in that, In step S3.2, the temperature of the dynamic circulating hot water extraction is 90-95℃, and the time is 2-3 hours.

8. The preparation process of a compound health drink containing Ganoderma lucidum according to claim 6, characterized in that, The natural flavoring agent in step S4.3 is composed of mogroside and ammonium glycyrrhizate in a mass ratio of 1-2:

1.

9. The preparation process of a compound health drink containing Ganoderma lucidum according to claim 6, characterized in that, The prebiotic in step S4.3 is composed of fructooligosaccharides and inulin in a mass ratio of 1-2:

1.

10. A compound health drink containing Ganoderma lucidum, characterized in that, It is prepared by the preparation process of a compound health drink containing Ganoderma lucidum as described in any one of claims 1-9.

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