Method for extracting and analyzing bamboo shoot skin dietary fibers by ultrasonic compound enzyme method

By using a compound enzymatic method to extract soluble and insoluble dietary fiber from bamboo shoot skin, the problems of dietary fiber structure damage and low extraction efficiency in existing technologies have been solved, achieving efficient and environmentally friendly dietary fiber extraction and obtaining high-quality dietary fiber products.

CN120918374APending Publication Date: 2025-11-11朱晓慧
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Patent Information

Application Number
CN202510712412.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-30
Publication Date
2025-11-11

AI Technical Summary

Technical Problem

Among existing methods for extracting dietary fiber, chemical methods cause significant damage to the fiber structure and result in severe environmental pollution; traditional physical methods are inefficient and energy-intensive; single ultrasonic extraction, if parameters are not properly controlled, can easily damage the fiber structure and affect its functional properties; and single enzymatic extraction has limited effectiveness and high cost. Therefore, how to efficiently separate and obtain high-quality soluble and insoluble dietary fiber is a problem that needs to be solved.

Method used

The method employs a complex enzymatic approach, including raw material pretreatment, enzymatic extraction, enzyme inactivation and separation, extraction of soluble and insoluble dietary fiber. A mixture of cellulase and xylanase is used, along with water and ethanol as solvents, to control mild reaction conditions, ensuring the integrity of the dietary fiber structure and extraction efficiency.

Benefits of technology

It achieves efficient and environmentally friendly extraction of high-quality soluble and insoluble dietary fiber, avoids the damage to the dietary fiber structure caused by high temperature and high pressure, improves the extraction rate and product purity, meets different application needs, and reduces the use of chemical reagents and waste emissions.

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Abstract

The invention discloses an extraction and analysis method of bamboo shoot skin dietary fibers. The method comprises the following specific steps: washing fresh bamboo shoot skin, drying at 70 DEG C until the weight is constant, crushing, sieving with a 80-mesh sieve, adding a proper amount of distilled water, adding 3.6% of compound enzyme in a proper proportion at 40 DEG C, reacting for 25 minutes, and inactivating in a water bath at 100 DEG C for 10 minutes; stirring and centrifuging to take supernate and precipitate, concentrating the supernate, adding four times of absolute ethyl alcohol, precipitating and filtering at the temperature of 4 DEG C, centrifuging, drying the precipitate to obtain soluble dietary fiber powder, eluting the precipitate with absolute ethyl alcohol, and drying to obtain an insoluble dietary fiber product. The extraction rate of soluble dietary fibers in the bamboo shoot skin is 18.38%, and the extraction rate of insoluble dietary fibers in the bamboo shoot skin is 46.42%. Soluble and insoluble dietary fibers are subjected to scanning electron microscope analysis, Fourier transform infrared spectroscopic analysis, X-ray diffraction analysis and antioxidant ability analysis to obtain characterization structure results of the soluble and insoluble dietary fibers. The process is simple and rapid, the extraction rate is high, the product purity is high, and the functionality is good.
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