Composition for reducing blood pressure, blood fat, blood sugar and uric acid and preparation method thereof

Abstract

The invention provides a composition for reducing blood pressure, blood fat, blood sugar and uric acid and a preparation method thereof, the composition takes nine kinds of vegetable fat such as acer truncatum seed oil, tomato seed oil and the like as core raw materials, a composite antioxidant, phytosterol and xylooligosaccharide are supplemented, multiple-effect synergy is realized through scientific proportioning, and in the preparation process, the composition is free of toxic and side effects. And by adopting a step-by-step stirring technology, a nitrogen protection technology and a gradient ultrasonic homogenization technology, the biological activity of each component is effectively maintained. A plurality of in-vitro experiments and animal models verify that the composition can significantly regulate blood pressure, blood fat, blood sugar and uric acid levels through the synergistic effect of the raw materials. The raw materials are natural, the active ingredients are completely reserved, and the efficacy is stable, so that the functional health-care product has a wide application prospect in the field of functional health-care product development.

Description

Technical Field

[0001] This invention relates to the field of functional food technology, and in particular to a composition for lowering blood pressure, blood lipids, blood sugar and uric acid and its preparation method. Background Technology

[0002] With changing lifestyles and an aging population, hypertension, hyperlipidemia, hyperglycemia ("the three highs") and hyperuricemia ("the four highs") have become common chronic metabolic diseases threatening public health, showing an increasing incidence, affecting younger people, and involving multiple conditions. These four conditions are interconnected and mutually influential. For example, hyperlipidemia can worsen atherosclerosis and induce high blood pressure; hyperglycemia can damage kidney function, leading to impaired uric acid excretion; and long-term hypertension can further exacerbate metabolic disorders of blood sugar and lipids, creating a vicious cycle. Therefore, developing products that can comprehensively regulate these four conditions simultaneously is of great significance for improving patients' health. Currently, the main methods for managing "four highs" (high blood pressure, high blood sugar, and high cholesterol) fall into two categories: chemical drug treatment and natural product therapy. While chemical drugs can take effect quickly, they suffer from limitations such as single-target therapy, long-term use leading to drug resistance and side effects (e.g., some lipid-lowering drugs may damage the liver, and uric acid-lowering drugs may cause gastrointestinal discomfort). Furthermore, they are difficult to simultaneously address the synergistic improvement of all four highs. Natural product therapy, due to its higher safety profile, has received widespread attention. Existing products primarily focus on single or combined effects, such as fish oil preparations for regulating blood lipids or mulberry leaf extract products for lowering blood sugar. Products offering comprehensive treatment for all four highs are relatively few. Existing natural product compositions have the following shortcomings: First, the raw material combinations lack systematic synergistic design, often involving simple mixing that fails to fully utilize the functional complementarity between different components. For example, some compositions containing plant oils rely solely on the unsaturated fatty acids of a single oil to exert their effects, without combining with the specific active ingredients of other oils, such as nervonic acid and chlorogenic acid, to form multi-target regulation. Second, the active ingredients of oil raw materials are not sufficiently preserved. Traditional extraction processes, such as high-temperature pressing and solvent extraction, easily lead to the oxidation and deterioration of active substances (vitamin E, lycopene) in the oils, affecting their efficacy. Third, bioavailability is limited. Some compositions are prone to stratification during storage due to the poor stability of the oil system, and are difficult to be efficiently absorbed after entering the human body, thus failing to fully exert their regulatory effects on the "four highs" (high blood pressure, high blood sugar, high cholesterol, and high blood sugar). Fourth, the intervention on the metabolic pathways associated with the "four highs" is not comprehensive enough. Most products only target a single metabolic link of a certain disease, such as only inhibiting cholesterol absorption, failing to form a multi-system regulatory mechanism involving the "blood vessels-liver-intestinal-kidneys," making it difficult to break the vicious cycle of mutual influence among the "four highs." Given the shortcomings of the existing technologies, developing a composition that uses multiple functional oils as its core, achieves synergistic effects through scientific formulation, retains active ingredients through optimized processes, and can comprehensively regulate the "four highs" (high blood pressure, high blood sugar, high cholesterol, and high uric acid) has become an urgent problem to be solved. Summary of the Invention

[0003] Therefore, this invention proposes a composition for lowering blood pressure, blood lipids, blood sugar and uric acid and its preparation method, thereby solving the above problems.

[0004] The technical solution of the present invention is achieved as follows: a composition for lowering blood pressure, blood lipids, blood sugar and uric acid, comprising a compound oil and an enhancer, wherein the compound oil comprises the following raw materials by weight percentage: 3-8% Acer truncatum seed oil, 1-3% tomato seed oil, 25-35% sea buckthorn seed oil, 20-30% milk thistle seed oil, 8-12% camellia seed oil, 10-15% peony seed oil, 2-5% Eucommia ulmoides seed oil, 3-5% perilla seed oil, and 5-10% sacha inchi oil.

[0005] Furthermore, a composition for lowering blood pressure, blood lipids, blood sugar and uric acid, wherein the compound oil comprises the following raw materials by weight percentage: 5% Acer truncatum seed oil, 2% tomato seed oil, 30% sea buckthorn seed oil, 25% milk thistle seed oil, 10% camellia seed oil, 12% peony seed oil, 4% eucommia seed oil, 4% perilla seed oil, and 8% sacha indica oil.

[0006] Furthermore, the reinforcing agent comprises the following raw materials in parts by weight: 3-8 parts of compound antioxidant, 2-4 parts of phytosterol, and 1-3 parts of xylooligosaccharide (degree of polymerization 2-7, purity ≥95%, food grade).

[0007] Furthermore, the composite antioxidant is puerarin and gallic acid in a mass ratio of (2.5-3.5):1.

[0008] Furthermore, the mass ratio of the composite oil to the reinforcing agent is 10:1-3.

[0009] Furthermore, a method for preparing a composition that lowers blood pressure, blood lipids, blood sugar, and uric acid includes the following steps: S1. Raw material pretreatment: After washing the sea buckthorn seeds, milk thistle seeds and other plant seeds, dry them at a low temperature of 40±2℃ until the moisture content is ≤5%, then crush them and pass them through a 50-70 mesh sieve, wherein the impurity removal rate of the raw materials is ≥99%; S2. Low-temperature supercritical CO2 extraction: Extract the above powder for 2-3 hours per batch. After extraction, reduce the pressure of the separation vessel to 5±1MPa and maintain the temperature at 28-32℃, and collect the lipid-soluble components. S3. Low-temperature mixing and homogenization: Add each oil phase to a nitrogen-protected stirred tank according to the ratio, stir at 150-250 rpm for 25-35 minutes at 20-30℃, and homogenize under high pressure to obtain mixed oil A. S4. Adding Enhancers: Add phytosterols to oil mixture A, heat to 40±2℃ and increase the rotation speed to 120-130 rpm. Sterol molecules are embedded in the gaps between oil molecules under shear force, forming a stable "oil-sterol" complex structure. Then, cool to 37±2℃, add xylooligosaccharides, and stir at 80-100 rpm for 7-9 minutes. The hydrophilicity of xylooligosaccharides allows them to be evenly distributed on the oil interface. Finally, add a complex antioxidant, cool to 35±2℃ and maintain the rotation speed at 60-70 rpm for 8-12 minutes to obtain oil mixture B. Utilize the molecular polarity of puerarin and gallic acid to adsorb onto the surface of oil particles to form an antioxidant protective layer. S5. Gradient ultrasonic-assisted homogenization: Mixed oil B is subjected to gradient ultrasonic-assisted homogenization under a nitrogen atmosphere to obtain the composition.

[0010] Furthermore, the extraction pressure of S2 is 25-30 MPa, the temperature is 35-40℃ (operation in the dark), and the CO2 flow rate is 12-15 L / min.

[0011] Furthermore, the homogenization pressure of S3 is 40-60 MPa, and the cycle is repeated 2-4 times.

[0012] Furthermore, the gradient ultrasound-assisted homogenization: First stage: Ultrasonic treatment at 18-22kHz frequency and 280-320W power for 1-3 minutes, with simultaneous stirring at 60-80rpm. Second stage: Adjust the frequency to 15-17kHz, reduce the power to 250-270W, and continue processing for 1-3 minutes; Phase 3: Increase the frequency to 20-24kHz and the power to 320-330W, and process for 1-2 minutes.

[0013] The cavitation effect generated by ultrasound can break up the agglomeration of residual tiny oil droplets, achieving molecular-level dispersion. The final particle size distribution deviation of the mixed system is ≤2μm, and the uniformity is improved to over 98%.

[0014] Furthermore, the composition is used in the preparation of health products for simultaneously lowering blood pressure, blood lipids, blood sugar and uric acid.

[0015] Compared with the prior art, the beneficial effects of the present invention are: Through the synergistic effect of the active ingredients in various seed oils, comprehensive regulation of hypertension, hyperlipidemia, hyperglycemia, and hyperuricemia is achieved. Among them, eucommia ulmoides seed oil contains eucommia flavonoids and chlorogenic acid, which inhibit angiotensin-converting enzyme activity to lower blood pressure; Acer truncatum seed oil and sea buckthorn seed oil improve blood lipids by regulating cholesterol metabolism and increasing high-density lipoprotein levels; perilla seed oil contains α-linolenic acid to enhance insulin sensitivity, and tomato seed oil inhibits the activity of key gluconeogenesis enzymes to regulate blood sugar; milk thistle seed oil inhibits xanthine oxidase activity to reduce uric acid production, and the antioxidant components of sea buckthorn seed oil reduce oxidative damage caused by uric acid, forming a "four-fold mechanism" to synergistically improve metabolic syndrome.

[0016] The compound antioxidants inhibit lipid oxidation, reduce the loss of active ingredients, and extend the shelf life of the composition, while enhancing its hypoglycemic and uric acid-lowering effects. Phytosterols can inhibit the absorption of cholesterol in the intestines, help lower blood lipids, and synergistically enhance the blood pressure-lowering effect of active ingredients in lipids, thus strengthening the stability of the composition in regulating blood lipids. Xylooligosaccharides, as prebiotics, can regulate intestinal flora, promote the excretion of metabolic waste, indirectly assist in lowering uric acid, and improve the dispersibility of the composition, enhancing the absorption efficiency of active ingredients. They also synergistically enhance the overall four-effect regulatory effect with other components. The combination of these three ingredients ensures the stability of the composition and strengthens its physiological activity. Detailed Implementation

[0017] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0018] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0019] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0020] Example 1 Raw material composition: Compound oil (1000g): 30g Acer truncatum seed oil, 30g tomato seed oil, 350g sea buckthorn seed oil, 200g milk thistle seed oil, 80g camellia seed oil, 150g peony seed oil, 20g eucommia seed oil, 30g perilla seed oil, and 90g sacha inchi oil; Enhancer formulation (100g): 50g compound antioxidant (puerarin and gallic acid mass ratio 2.5:1), 30g phytosterols, 20g xylooligosaccharides; Example 2 Compound oil (1000g): 80g Acer truncatum seed oil, 10g tomato seed oil, 250g sea buckthorn seed oil, 300g milk thistle seed oil, 120g camellia seed oil, 100g peony seed oil, 50g eucommia seed oil, 50g perilla seed oil, and 40g sacha inchi oil; Enhancer formulation (300g): 160g compound antioxidant (puerarin and gallic acid mass ratio 3.5:1), 80g phytosterols, and 60g xylooligosaccharides.

[0021] Example 3 Raw material composition: Compound oil (1000g): 50g Acer truncatum seed oil, 20g tomato seed oil, 300g sea buckthorn seed oil, 250g milk thistle seed oil, 100g camellia seed oil, 120g peony seed oil, 40g eucommia seed oil, 40g perilla seed oil, and 80g sacha inchi oil. Enhancer formulation (200g): 100g compound antioxidant (puerarin and gallic acid mass ratio 3:1), 60g phytosterols, and 40g xylooligosaccharides; The above Examples 1-3 were prepared using the following methods: S1. Raw material pretreatment: After washing the sea buckthorn seeds, milk thistle seeds and other plant seeds, dry them at a low temperature of 40℃ until the moisture content is ≤5%, then crush them and pass them through a 60-mesh sieve, wherein the impurity removal rate of the raw materials is ≥99%; S2. Low-temperature supercritical CO2 extraction: The above powder is extracted at a pressure of 25-30 MPa, a temperature of 35-40℃ (operated in the dark), and a CO2 flow rate of 12-15 L / min for 2-3 hours per batch. After extraction, the pressure of the separation vessel is reduced to 5 MPa and the temperature is maintained at 28-32℃. The fat-soluble components are collected. S3. Low-temperature mixing and homogenization: Add each oil phase to a nitrogen-protected stirring vessel according to the formula, stir at 150-250 rpm for 25-35 minutes at 20-30℃, and circulate 2-4 times at a homogenization pressure of 40-60 MPa to obtain mixed oil A. S4. Adding enhancer: Add phytosterols to oil mixture A, heat to 40°C and increase the speed to 125 rpm, then cool to 37°C, add xylooligosaccharides, and stir at 90 rpm for 8 minutes; finally add compound antioxidants, cool to 35°C and stir at 65 rpm for 10 minutes to obtain oil mixture B. S5. Homogenize oil mixture B under a nitrogen atmosphere using gradient ultrasonication: First stage: Ultrasonic treatment at 20kHz frequency and 300W power for 2 minutes, with simultaneous stirring at 70rpm; Second stage: Adjust the frequency to 16kHz, reduce the power to 260W, and continue processing for 2 minutes; Phase 3: Increase frequency to 22kHz, power to 325W, and process for 1 minute.

[0022] Comparative Example 1 The difference between this comparative example and Example 3 is that the raw material composition lacks tomato seed oil, perilla seed oil, and sacha inchi oil, and is supplemented with Acer truncatum seed oil, sea buckthorn seed oil, and camellia seed oil, specifically: Raw material composition: Compound oil (1000g): 90g of Acer truncatum seed oil, 350g of Hippophae rhamnoides seed oil, 250g of Milk thistle seed oil, 150g of Camellia oleifera seed oil, 120g of Peony seed oil, and 40g of Eucommia ulmoides seed oil; Enhancer formulation (200g): 100g compound antioxidant (puerarin and gallic acid in a mass ratio of 3:1), 60g phytosterols, and 40g xylooligosaccharides.

[0023] Comparative Example 2 The difference between this comparative example and Example 3 is that the raw material composition lacks milk thistle seed oil and eucommia seed oil, and uses Acer truncatum seed oil and sea buckthorn seed oil to make up for them, specifically: Raw material composition: Compound oil (1000g): 300g Acer truncatum seed oil, 20g tomato seed oil, 340g sea buckthorn seed oil, 100g Camellia chinensis seed oil, 120g peony seed oil, 40g perilla seed oil, and 80g Sacha inchi oil; Enhancer formulation (200g): 100g compound antioxidant (puerarin and gallic acid in a mass ratio of 3:1), 60g phytosterols, and 40g xylooligosaccharides.

[0024] Comparative Example 3 The difference between this comparative example and Example 3 is that the composite antioxidant contains only puerarin.

[0025] Comparative Example 4 The difference between this comparative example and Example 3 is that gradient ultrasonic-assisted homogenization was not performed when mixing oil B; conventional homogenization (20kHz / 300W×5min) was used instead. The other steps were the same as in Example 3.

[0026] Experimental Example 1 - Blood Pressure Lowering Effect Test 1. Experimental subjects: Sixty healthy SD rats were selected and induced into a hypertensive rat model. The successfully induced rats were randomly divided into 6 groups of 10 rats each: Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and blank control group (gavaged with an equal volume of physiological saline).

[0027] Specific induction method: subcutaneous injection of deoxycorticosterone acetate (DOCA) combined with a high-salt diet / tail vein injection of angiotensin II; 2. Experimental Methods: Rats in each group were administered the corresponding composition by gavage at a dose of 10 mL / kg body weight once daily for 4 consecutive weeks. The systolic blood pressure of the rat's tail artery was measured before gavage, 2 weeks after gavage, and 4 weeks after gavage.

[0028] 3. Test Results

[0029] Experimental Example 2 - Test of Lipid-Lowering Effect 1. Experimental subjects: Sixty rats with hyperlipidemia induced by high-fat diet were selected and randomly divided into 6 groups of 10 rats each. The grouping method was the same as in Experiment 1.

[0030] 2. Experimental Methods: Each group was administered the corresponding composition by gavage at a dose of 10 mL / kg body weight once daily for 4 consecutive weeks, during which time the rats were continuously fed a high-fat diet. After 4 weeks of gavage, the serum total cholesterol (TC) and triglyceride (TG) levels in rats were measured.

[0031] 3. Test Results Example 3 group: TC content was 2.8 mmol / L and TG content was 1.2 mmol / L, which were significantly lower than before gavage (TC 5.6 mmol / L, TG 2.8 mmol / L). Comparative Example 1: TC content 3.5 mmol / L, TG content 1.6 mmol / L, lipid-lowering effect was weaker than Example 3. Comparative Example 2: TC content 3.3 mmol / L, TG content 1.5 mmol / L, the lipid-lowering effect was not as good as Example 3. Comparative Example 3: TC content 3.2 mmol / L, TG content 1.4 mmol / L, the lipid-lowering effect was slightly worse than that of Example 3. Comparative Example 4: TC content 3.1 mmol / L, TG content 1.3 mmol / L, lipid-lowering effect slightly lower than Example 3. Blank control group: TC content 5.5 mmol / L, TG content 2.7 mmol / L, no significant change.

[0032] Experimental Example 3 - Blood Glucose Lowering Effect Test 1. Experimental subjects: Sixty streptozotocin-induced diabetic rats (fasting blood glucose ≥11.1 mmol / L) were selected and randomly divided into 6 groups of 10 rats each. The grouping method was the same as in Experiment 1. 2. Experimental Methods: Each group was administered the corresponding composition by gavage at a dose of 10 mL / kg body weight once daily for 4 consecutive weeks. Fasting blood glucose levels were measured in rats before gavage and 4 weeks after gavage.

[0033] 3. Test Results

[0034] Experimental Example 4 - Uric Acid Lowering Effect Test 1. Experimental subjects: Sixty rats with hyperuricemia induced by potassium oxonate were selected and randomly divided into 6 groups of 10 rats each. The grouping method was the same as in Experiment 1. 2. Experimental Methods: Each group was administered the corresponding composition by gavage at a dose of 10 mL / kg body weight once daily for 4 consecutive weeks. Serum uric acid levels in rats were measured after 4 weeks of gavage.

[0035] 3. Experimental Results:

[0036] The experimental results above show that the composition of Example 3 exhibits the best effects in lowering blood pressure, blood lipids, blood sugar, and uric acid. The efficacy of Comparative Examples 1-4 decreased due to missing raw materials, altered composite antioxidant components, or simplified preparation processes. This indicates that the raw material ratio and preparation method of Example 3 are reasonable and have a synergistic effect.

[0037] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A composition for lowering blood pressure, blood lipids, blood sugar, and uric acid, characterized in that, It includes compound oil and reinforcing agent, wherein the compound oil comprises the following raw materials by weight percentage: 3-8% Acer truncatum seed oil, 1-3% tomato seed oil, 25-35% sea buckthorn seed oil, 20-30% milk thistle seed oil, 8-12% camellia seed oil, 10-15% peony seed oil, 2-5% eucommia seed oil, 3-5% perilla seed oil, and 5-10% sacha inchi oil.

2. The composition for lowering blood pressure, blood lipids, blood sugar, and uric acid as described in claim 1, characterized in that, The compound oil comprises the following raw materials by weight percentage: 5% Acer truncatum seed oil, 2% tomato seed oil, 30% sea buckthorn seed oil, 25% milk thistle seed oil, 10% camellia seed oil, 12% peony seed oil, 4% eucommia seed oil, 4% perilla seed oil, and 8% sacha inchi oil.

3. The composition for lowering blood pressure, blood lipids, blood sugar, and uric acid as described in claim 1, characterized in that, The reinforcing agent comprises the following raw materials in parts by weight: 3-8 parts of compound antioxidant, 2-4 parts of phytosterol, and 1-3 parts of xylooligosaccharide.

4. The composition for lowering blood pressure, blood lipids, blood sugar, and uric acid as described in claim 1, characterized in that, The composite antioxidant is puerarin and gallic acid in a mass ratio of (2.5-3.5):

1.

5. The composition for lowering blood pressure, blood lipids, blood sugar, and uric acid as described in claim 1, characterized in that, The mass ratio of the composite oil to the reinforcing agent is 10:1-3.

6. A method for preparing a composition for lowering blood pressure, lowering blood lipids, lowering blood sugar, and lowering uric acid as described in any one of claims 1-5, characterized in that, Includes the following steps: S1. Raw material pretreatment: After washing the sea buckthorn seeds, milk thistle seeds and other plant seeds, dry them at a low temperature of 40±2℃ until the moisture content is ≤5%, then crush them and pass them through a 50-70 mesh sieve, wherein the impurity removal rate of the raw materials is ≥99%; S2. Low-temperature supercritical CO2 extraction: Extract the above powder for 2-3 hours per batch. After extraction, reduce the pressure of the separation vessel to 5±1MPa and maintain the temperature at 28-32℃, and collect the lipid-soluble components. S3. Low-temperature mixing and homogenization: Add each oil phase to a nitrogen-protected stirred tank according to the ratio, stir at 150-250 rpm for 25-35 minutes at 20-30℃, and homogenize under high pressure to obtain mixed oil A. S4. Adding enhancer: Add phytosterols to oil mixture A, heat to 40±2℃ and increase the speed to 120-130 rpm, then cool to 37±2℃, add xylooligosaccharides, and stir at 80-100 rpm for 7-9 minutes; finally add compound antioxidants, cool to 35±2℃ and maintain the speed at 60-70 rpm for 8-12 minutes to obtain oil mixture B; S5. Gradient ultrasonic-assisted homogenization: Mixed oil B is subjected to gradient ultrasonic-assisted homogenization under a nitrogen atmosphere to obtain the composition.

7. The method for preparing a composition for lowering blood pressure, blood lipids, blood sugar, and uric acid as described in claim 6, characterized in that, The extraction pressure of S2 is 25-30 MPa, the temperature is 35-40℃, and the CO2 flow rate is 12-15 L / min.

8. The method for preparing a composition for lowering blood pressure, blood lipids, blood sugar, and uric acid as described in claim 6, characterized in that, The homogenization pressure of S3 is 40-60 MPa, and the cycle is 2-4 times.

9. The method for preparing a composition for lowering blood pressure, blood lipids, blood sugar, and uric acid as described in claim 6, characterized in that: The gradient ultrasound-assisted homogenization: First stage: Ultrasonic treatment at 18-22kHz frequency and 280-320W power for 1-3 minutes, with simultaneous stirring at 60-80rpm. Second stage: Adjust the frequency to 15-17kHz, reduce the power to 250-270W, and continue processing for 1-3 minutes; Phase 3: Increase the frequency to 20-24kHz and the power to 320-330W, and process for 1-2 minutes.

10. The use of the composition according to any one of claims 1-5 in the preparation of a health product for simultaneously lowering blood pressure, blood lipids, blood glucose and uric acid.

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