Application of GhHAT18 gene in regulation and control of drought stress resistance of plants

Abstract

The invention belongs to the technical field of gene engineering, and particularly relates to application of a GhHAT18 gene in regulation and control of drought stress resistance of plants, the nucleotide sequence of the GhHAT18 gene is shown as SEQ ID NO.1. The invention discloses that the cotton histone acetylase gene GhHAT18 can be used for regulating and controlling the drought resistance of the plants for the first time, and experiments show that compared with a wild type plant, after the expression of the GhHAT18 gene is inhibited, the GhHAT18 gene can be used for regulating and controlling the drought stress resistance of the plants. The antioxidant enzyme activity and the drought resistance of plants can be obviously reduced; and the transgenic plant over-expressed with the GhHAT18 shows stronger and higher antioxidant enzyme activity and drought resistance under the drought stress. Transcriptome analysis shows that the GhHAT18 gene affects the expression of drought-resistant related genes through a secondary metabolite synthesis pathway and an MAPK signal transduction pathway, and the adaptive capacity of plants to drought stress is further regulated and controlled.

Description

Technical Field

[0001] This invention belongs to the field of genetic engineering technology, specifically involving GhHAT18 Application of genes in regulating plant resistance to drought stress. Background Technology

[0002] cotton( Gossypium hirsutum Cotton is a globally important economic crop, widely used in the textile, oilseed, and feed industries, playing an indispensable role in the global economy and human life. However, drought stress is one of the major abiotic stresses affecting cotton yield and quality, leading to weakened photosynthesis, decreased root vitality, and disordered antioxidant systems, thus severely restricting cotton yield and quality and causing huge losses to the cotton industry.

[0003] Currently, various methods have been developed to address the drought stress on cotton, including optimized cultivation management, external chemical regulation, and screening for drought-resistant cotton varieties. Regarding cultivation management, while measures such as proper irrigation and hilling can alleviate the impact of drought to some extent, they require significant manpower and resources and are heavily constrained by environmental conditions. In terms of chemical regulation, plant growth regulators can regulate cotton growth, but some regulators leave residues that may pose potential harm to the environment and cotton quality. Screening for drought-resistant cotton varieties is time-consuming, inefficient, and involves complex genetic backgrounds, making it difficult to quickly respond to the challenges posed by climate change. Therefore, identifying key genes in cotton that can regulate drought stress is crucial for improving cotton's drought resistance and breeding drought-resistant cotton varieties, providing new ideas and methods for solving this agricultural problem. Summary of the Invention

[0004] To address the above problems, the present invention provides GhHAT18 The application of genes in regulating plant drought stress resistance: the discovery of silencing. GhHAT18 Gene expression can reduce a plant's resistance to drought stress, while overexpression of this gene can reduce its resistance to drought stress. GhHAT18 Genes can enhance a plant's resistance to drought stress.

[0005] To achieve the above objectives, the specific technical solution of the present invention is as follows: The first aspect of the present invention provides GhHAT18 The application of genes in regulating plant drought stress resistance, the aforementioned GhHAT18 The nucleotide sequence of the gene is shown in SEQ ID NO.1.

[0006] Furthermore, the application is: to improve the quality of plants through transgenic technology or gene editing technology. GhHAT18 The expression level of genes can be adjusted to enhance the plant's resistance to drought stress; or gene knockout or silencing can be used to enhance the plant's resistance to drought stress. GhHAT18Genes to reduce the plant's ability to resist drought stress.

[0007] Furthermore, silence. GhHAT18 The gene was generated using VIGS technology.

[0008] Furthermore, the plant is cotton, Arabidopsis thaliana, rapeseed, wheat, rice, or corn.

[0009] A second aspect of this invention provides the application of the GhHAT18 protein in regulating plant drought stress resistance, wherein the GhHAT18 protein is derived from the above-described... GhHAT18 The gene encodes the GhHAT18 protein, and the amino acid sequence of the protein is shown in SEQ ID NO.2.

[0010] Furthermore, the plant is cotton, Arabidopsis thaliana, rapeseed, wheat, rice, or corn.

[0011] A third aspect of the present invention provides the application of a recombinant expression vector in regulating plant drought stress resistance, wherein the recombinant expression vector contains the above-described... GhHAT18 .

[0012] Furthermore, the starting vector of the recombinant vector is WMV067, pCAMBIA2300, pCAMBIA1301 or PBI121.

[0013] Furthermore, the plant is cotton, Arabidopsis thaliana, rapeseed, wheat, rice, or corn.

[0014] A fourth aspect of the present invention provides the application of a recombinant bacterium in regulating plant resistance to drought stress, wherein the recombinant bacterium contains the above-described... GhHAT18 .

[0015] Furthermore, the starting strain of the recombinant bacteria is a prokaryotic microorganism or a eukaryotic microorganism.

[0016] Furthermore, the plant is cotton, Arabidopsis thaliana, rapeseed, wheat, rice, or corn.

[0017] The fifth aspect of this invention provides a method for cultivating drought-resistant transgenic plants, comprising the following steps: The above-mentioned GhHAT18 Genes are linked to expression vectors to construct recombinant expression vectors; The recombinant expression vector was transformed into Agrobacterium to obtain recombinant Agrobacterium; By infecting the target plant with the recombinant Agrobacterium, drought-resistant transgenic plants were obtained.

[0018] Furthermore, the expression vector is WMV067, pCAMBIA2300, pCAMBIA1301, or PBI121.

[0019] Furthermore, the Agrobacterium is GV3101, EHA105, or LBA4404.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention is disclosed for the first time. GhHAT18 Genes can be used to regulate a plant's resistance to drought stress. GhHAT18 The nucleotide sequence of the gene is shown in SEQ ID NO.1. GhHAT18 The amino acid sequence of the gene-encoded protein is shown in SEQ ID NO.2. This invention demonstrates experimentally that: inhibition... GhHAT18 After gene expression, the drought resistance of cotton plants was significantly reduced, manifested as accelerated leaf wilting, decreased antioxidant enzyme activity, and reduced accumulation of osmotic regulatory substances; while in Arabidopsis thaliana, overexpression of the gene significantly reduced the drought resistance of cotton plants. GhHAT18 After gene overexpression, compared with wild-type plants, GhHAT18 The transgenic plants exhibited higher antioxidant enzyme activity and greater accumulation of osmotic regulatory substances under drought stress. Further transcriptome analysis revealed… GhHAT18 Genes influence the expression of drought-related genes through secondary metabolite synthesis pathways and the MAPK signaling pathway, thereby regulating plant adaptation to drought stress. Utilizing... GhHAT18 Genes can be used to genetically modify a plant's ability to resist drought stress.

[0021] GhHAT18 The gene is a cotton histone acetyltransferase gene. Current research on plant drought resistance largely focuses on transcriptional regulation, but systematic studies on the correlation between epigenetic mechanisms (such as histone modifications) and drought resistance are still relatively scarce. Histone acetylation modifications participate in plant stress responses by regulating chromatin structure and gene expression, but the specific function and molecular mechanism of the cotton histone acetyltransferase gene in drought resistance have not been clearly reported. This invention reveals for the first time that the histone acetyltransferase gene can be used to regulate cotton drought resistance, providing new gene resources and theoretical basis for molecular breeding related to drought resistance. It fills the research gap on the correlation between cotton histone acetyltransferase and plant drought resistance, and also provides an efficient and sustainable solution for drought resistance breeding of cotton and other crops, possessing significant theoretical value and application prospects. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This section presents an expression analysis of the cotton histone acetyltransferase gene GhHATs; Figure A is a heatmap showing the expression patterns of the HATs family genes in cotton under polyethylene glycol treatment conditions, with the red box indicating... GhHAT18 Gene expression at different polyethylene glycol treatment times; Figure B shows... GhHAT18 Gene expression under normal watering and natural drought stress, CK represents normal watering treatment, Drought represents drought treatment, and ** indicates p <0.01.

[0024] Figure 2 for GhHAT18 Experimental analysis of the effect of gene silencing on drought stress tolerance in cotton. TRV2:00 represents control plants, TRV2: GhHAT18 for GhHAT18 Image A shows the albino phenotype of gene-silenced positive control plants; Image B shows the qPCR analysis of control plants and... GhHAT18 In gene-silenced plants GhHAT18 A statistical graph of gene expression levels, *** indicates p <0.001; The top and bottom images in Figure C are the control plant and the control plant, respectively. GhHAT18 Phenotypic characteristics of gene-silenced plants at 0 days of drought and 14 days of drought stress.

[0025] Figure 3 For control plants and GhHAT18 Statistical results of physiological indicators of gene-silenced cotton plants under drought stress. TRV2:00 represents the control plant, TRV2: GhHAT18 for GhHAT18 Gene-silenced plants, CK represents normal watering treatment, Drought represents drought treatment; Figure A shows... GhHAT18 The effect of gene silencing on proline content in plants, Figure B. GhHAT18 The effect of gene silencing on malondialdehyde (MDA) contents in plants, as shown in Figure C. GhHAT18 The effect of gene silencing on catalase activity (CAT activity) in plants, as shown in Figure D. GhHAT18 The effect of gene silencing on catalase activity (POD activity) in plants, as shown in Figure E. GhHAT18 The effect of gene silencing on superoxide dismutase (SOD) activity in plants, ** indicates p <0.01, **** indicates p <0.001.

[0026] Figure 4For wild type and overexpression GhHAT18 Phenotypic analysis of transgenic Arabidopsis thaliana under drought stress: WT represents wild-type Arabidopsis thaliana, while L2, L13, and L8 represent overexpression. GhHAT18 Three transgenic Arabidopsis thaliana lines; Figure A shows... GhHAT18 Genes in wild type and overexpression GhHAT18 Statistical graph of expression levels in transgenic Arabidopsis thaliana, *** indicates p <0.001; Figure B shows wild-type and overexpression. GhHAT18 Phenotypic characteristics of transgenic Arabidopsis plants after 0 days of drought and after 14 days of drought.

[0027] Figure 5 For wild type and overexpression GhHAT18 Analysis of physiological parameters of transgenic Arabidopsis thaliana under drought stress: WT represents wild-type Arabidopsis thaliana, while L2, L13, and L8 represent overexpression. GhHAT18 Three transgenic Arabidopsis lines are shown, with CK representing normal watering and Drought representing drought treatment; Figure A shows overexpression. GhHAT18 The effect of gene expression on proline content in Arabidopsis thaliana; Figure B shows overexpression. GhHAT18 Effect of gene on catalase activity in Arabidopsis thaliana, Figure C shows overexpression. GhHAT18 The effect of the gene on catalase activity (POD activity) in Arabidopsis thaliana; Figure D shows overexpression. GhHAT18 The effect of genes on malondialdehyde (MDA) contents in Arabidopsis thaliana; * indicates p <0.05, ** indicates p <0.01, *** indicates p <0.001.

[0028] Figure 6 For control plants and GhHAT18 Transcriptome analysis of gene-silenced plants under drought stress; Figure A shows a visualization of differentially expressed genes using a volcano plot, with red dots indicating gene expression. GhHAT18 Genes whose expression levels were upregulated in gene-silenced plants compared to control plants are indicated by green dots. GhHAT18 Genes whose expression levels were downregulated in gene-silenced plants compared to control plants; Figure B shows the expression levels of control plants and... GhHAT18 KEGG enrichment analysis results of differentially expressed genes among gene-silenced plants (shown in blue boxes). GhHAT18 Genes may regulate secondary biosynthetic pathways and MAPK signaling pathways. Detailed Implementation

[0029] The specific embodiments of the present invention are described in detail below, but it should be understood that the scope of protection of the present invention is not limited to the specific embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Unless otherwise specified, the experimental methods described in the embodiments of the present invention are conventional methods, and the materials and reagents used in the following embodiments are commercially available unless otherwise specified.

[0030] The cotton variety used in this embodiment of the invention is TM-1.

[0031] Cotton is a vital global economic crop, and drought stress is one of the major abiotic stresses affecting cotton yield and quality, severely restricting both. Currently, methods to address drought stress in cotton mainly include optimized cultivation management, external chemical regulation, and screening for drought-resistant cotton varieties. However, these methods suffer from limitations such as low efficiency, high environmental risks, and long breeding cycles. Therefore, identifying key genes in cotton that can regulate drought stress is of great significance for improving cotton's drought resistance and breeding drought-resistant cotton varieties.

[0032] This invention provides GhHAT18 This invention, through the integration of virus-induced gene silencing (VIGS) technology and overexpression system, has for the first time discovered and validated a cotton histone acetyltransferase gene in regulating plant drought stress. GhHAT18 It plays an important role in regulating plant drought resistance. Experimental results show that using VIGS technology to inhibit... GhHAT18 After gene expression, the drought resistance of cotton plants was significantly reduced, manifested as accelerated leaf wilting, decreased antioxidant enzyme activity, and reduced accumulation of osmotic regulatory substances; while in Arabidopsis thaliana, overexpression of the gene significantly reduced the drought resistance of cotton plants. GhHAT18 Following the gene transfer, transgenic plants exhibited better growth and higher antioxidant enzyme activity under drought stress. Further transcriptome analysis revealed that... GhHAT18 Genes enhance plants’ ability to adapt to drought stress by influencing the synthesis of secondary metabolites and the MAPK signaling pathway, as well as the expression of drought-related genes.

[0033] Example 1: Screening of candidate genes regulating plant drought stress resistance Histone acetyltransferase genes were analyzed using transcriptome data from cotton leaves under polyethylene glycol treatment (simulated drought) conditions. GhHATs The expression status was analyzed using qPCR. GhHAT18 Gene expression under natural drought stress.

[0034] The results are as follows Figure 1As shown in Figure A, the expression of most histone acetyltransferase genes did not change significantly after one hour of polyethylene glycol treatment. GhHAT18 The expression was most significantly upregulated, indicating that... GhHAT18 It may have participated in the cotton's response to drought stress. For example... Figure 1 As shown in B in the text GhHAT18 Gene expression was also significantly induced by natural drought stress.

[0035] GhHAT18 The nucleotide sequence of the gene is shown in SEQ ID NO.1. GhHAT18 The amino acid sequence of the protein encoded by the gene is shown in SEQ ID NO.2.

[0036] SEQ ID NO.1:

[0037] SEQ ID NO.2: .

[0038] Example 2: Silence GhHAT18 Genetic verification of its relationship with plant drought stress resistance Using virus-induced gene silencing technology (VIGS) to GhHAT18 To investigate the role of this gene in cotton drought resistance, silencing was performed. The VIGS experiment used the dual expression vectors TRV1 and TRV2 of tobacco rattle virus (TRV). TRV1 carries genes essential for viral replication and transport, serving as a helper viral vector within the VIGS system. TRV2 contains encoding essential viral coat proteins and multiple cloning sites (MCS), which facilitate the insertion of the target gene. GhCLA The gene is known for its high conservation and its role in chloroplast development. When silenced, it exhibits a unique albino phenotype and can therefore serve as a positive control for VIGS experiments.

[0039] First of all GhHAT18The silent gene fragment is inserted into the multiple cloning site of the TRV2 vector to form TRV2: GhHAT18 Recombinant expression vectors containing TRV1, TRV2 (empty vector), and TRV2: GhHAT18 The recombinant vectors were transformed into Agrobacterium GV3101. Agrobacterium suspensions containing TRV1 and TRV2 (empty vectors) were mixed and injected into cotton cotyledons to obtain control plants (TRV2:00). The suspensions containing TRV1 and TRV2 were then injected into cotton cotyledons. GhHAT18 After mixing the Agrobacterium tumefaciens bacterial suspension with the recombinant vector, it was injected into the cotyledons of cotton to obtain... GhHAT18 Gene-silenced cotton plants (TRV2: GhHAT18 ).

[0040] Further selection was made of control plants with uniform growth (TRV2:00) and GhHAT18 Gene-silenced cotton plants (TRV2: GhHAT18 ) was analyzed, and control plants and GhHAT18 Gene-silenced cotton plants were watered every three days with the same amount of water each time. Four-week-old cotton plants were subjected to a drought treatment; the fourth day after the last watering was recorded as drought day 0, after which no further watering was given, and the plants were allowed to dry naturally. The treatment continued until the control plants and... GhHAT18 When differences in phenotype were observed in gene-silenced cotton plants, photographs were taken to record the growth status of the two groups of cotton before and after drought treatment.

[0041] The results are as follows Figure 2 As shown, after drought treatment, GhHAT18 The growth of gene-silenced cotton plants was significantly worse than that of control plants, with noticeable wilting of their leaves.

[0042] This invention further utilizes the total superoxide dismutase (T-SOD) assay kit, catalase (CAT) assay kit, peroxidase (POD) assay kit, and proline (Pro) assay kit from the Nanjing Jiancheng Biotechnology Research Institute, following the kit instructions, to measure the levels of control plants and... GhHAT18 Physiological indicators of gene-silenced cotton plants under drought stress.

[0043] The results are as follows Figure 3 As shown, silence GhHAT18 The proline content, catalase activity, catalase activity, and superoxide dismutase activity of the cotton plants were all lower than those of the control plants, indicating that the silencing of proline content was a contributing factor. GhHAT18 The above results significantly reduced the cotton's tolerance to drought stress, indicating that... GhHAT18 It plays an important role in the cotton drought response process.

[0044] Example 3: Overexpression GhHAT18 Genetic verification of its relationship with plant drought stress resistance RNA was extracted from cotton leaves and reverse transcribed into cDNA using a reverse transcription kit.

[0045] according to GhHAT18 The nucleotide sequence of the gene, designed for amplification GhHAT18 The primer set for the gene includes an upstream primer with a nucleotide sequence as shown in SEQ ID NO.3, and a downstream primer with a nucleotide sequence as shown in SEQ ID NO.4.

[0046] Using cDNA as a template, amplification GhHAT18 The primer set for the gene was used for amplification. Amplification was performed according to the following PCR reaction system and procedure to obtain the PCR product. The PCR product was then subjected to agarose gel electrophoresis, and the correctly positioned and bright bands were selected for gel extraction and recovery.

[0047] SEQ ID NO.3: 5'-atgaccatgattacgaattcGGATGGCAACTGCCGCCATAGC-3'; SEQ ID NO. 4: 5'-aggtcgactctagaggatccCTATGAAGTCAGATATTTAACC-3'.

[0048] Each 25 μL PCR reaction system contains: 2 μL cDNA, 12.5 μL PCR MIX reagent, 1 μL upstream primer, 1 μL downstream primer, and 8.5 μL ddH2O.

[0049] PCR reaction program: 94℃ for 5 min; 94℃ for 30 s, 58℃ for 30 s, 72℃ for 60 s, 30 cycles; 72℃ for 5 min.

[0050] The pCAMBIA1301 vector was digested twice at 37°C for 45 min to obtain the digested pCAMBIA1301 vector.

[0051] pCAMBIA1301 vector double digestion system: Buffer 3μL, EcoR 1μL BamH Add 1 μL of I and 2 μL of pCAMBIA1301 carrier, then add ddH2O to bring the total volume to 30 μL.

[0052] The recovered DNA fragment was mixed with the enzyme-digested pCAMBIA1301 vector and reacted in a 50°C water bath for 15 min to obtain the ligation product. This ligation product was transformed into *E. coli* DH5α competent cells. Colony PCR and sequencing were used to screen for recombinant expression vectors containing the target fragment. GhHAT18 The recombinant expression vector pCAMBIA1301- GhHAT18 Finally, the recombinant expression vector pCAMBIA1301- GhHAT18 The bacteria were transformed into Agrobacterium GV3101 competent cells to obtain recombinant bacteria.

[0053] Seed sterilization and culture: Arabidopsis seeds were stored in the dark at 4°C for two days before germination. After sterilization with sodium hypochlorite and rinsing with sterile water, the seeds were placed in 1 / 2 MS medium and allowed to germinate under long-day conditions (16h light / 8h dark) at 23°C. During the seedling stage, the plants were grown in a substrate of nutrient soil and vermiculite in a 3:1 mass ratio. Arabidopsis were cultured in an incubator at 26°C with a light / dark cycle of 16h light and 8h dark.

[0054] Preparation of infection solution: The obtained recombinant bacteria were added to LB liquid medium containing 0.05 mg / mL kanamycin and 0.05 mg / mL rifampin, and activated in a shaker at 28°C and 220 rpm for 16 h. The bacteria were then resuspended in MS liquid medium (0.21 g MS salt, 5 g sucrose, 10 μL Silwet L77 to a final volume of 100 mL). The OD of the bacterial solution was then calculated. 600 The value was adjusted to 0.5 to obtain the inoculum.

[0055] Infection and Culture: Arabidopsis inflorescences were immersed in the infection solution, gently shaken for 3 seconds, and incubated in the dark for 24 hours. One week later, the infection process was repeated. Once mature, the first collection of mature seeds (T0 generation) was performed. These T0 seeds were then planted on 1 / 2 MS medium containing 0.035 mg / mL hygromycin for selection. The selected green seedlings were transplanted into soil for further culture, and DNA was extracted for positive detection. The results were then compared between the wild type and the detected positive lines. GhHAT18 Gene expression levels were assessed, and lines with significantly upregulated expression were selected for subculturing. Transgenic plants were then subjected to drought stress treatment and physiological parameter measurements after subculturing to the T3 generation.

[0056] WT represents wild-type Arabidopsis thaliana, while L2, L13, and L8 represent overexpressed strains. GhHAT18 Three strains of transgenic Arabidopsis thaliana.

[0057] overexpression GhHAT18 Phenotypic and physiological parameters of transgenic Arabidopsis thaliana under drought stress are analyzed as follows: Figure 4 and5 As shown: Compared to wild-type plants, overexpression GhHAT18 The transgenic plants exhibited better growth under drought stress. After drought stress treatment, overexpression... GhHAT18 The transgenic plants had higher proline content, catalase activity, and catalase activity than the wild-type plants.

[0058] Example 4: GhHAT18 Influencing cotton drought resistance by regulating secondary metabolism and the MAPK signaling pathway The present invention further compares the control plants and the plants prepared in Example 2. GhHAT18 Transcriptome sequencing analysis was performed on silent plants.

[0059] The results are as follows Figure 6 As shown, silence GhHAT18 This resulted in the upregulation of 1387 genes and the downregulation of 1541 genes. Figure 6 Further KEGG enrichment analysis of differentially expressed genes (A) revealed that they were mainly concentrated in the secondary biosynthetic pathway and the MAPK signaling pathway. Figure 6 (B in the text) indicates that GhHAT18 It may regulate genes in these two signaling pathways, thereby responding to cotton drought stress.

[0060] It should be noted that when numerical ranges are involved in this invention, it should be understood that both endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods used are the same as in the embodiments, preferred embodiments are described here to avoid redundancy. Although preferred embodiments of the invention have been described, those skilled in the art, once they understand the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of this invention.

[0061] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A kind GhHAT18 The application of genes in regulating plant drought stress resistance is characterized by, The GhHAT18 The nucleotide sequence of the gene is shown in SEQ ID NO.

1.

2. As described in claim 1 GhHAT18 The application of genes in regulating plant drought stress resistance is characterized by, Improving the quality of plants through transgenic or gene-editing technologies GhHAT18 The expression level of genes can be adjusted to improve the plant's resistance to drought stress.

3. As described in claim 1 GhHAT18 The application of genes in regulating plant drought stress resistance is characterized by, The plants mentioned are cotton, Arabidopsis thaliana, rapeseed, wheat, rice, or corn.

4. The application of a GhHAT18 protein in regulating plant drought stress resistance, characterized in that, The GhHAT18 protein is as described in claim 1. GhHAT18 Gene encoding; the plant is cotton, Arabidopsis thaliana, rapeseed, wheat, rice or corn.

5. The application of a recombinant expression vector in regulating plant drought stress resistance, characterized in that, The recombinant expression vector contains the features described in claim 1. GhHAT18 Genes; the plants mentioned are cotton, Arabidopsis thaliana, rapeseed, wheat, rice, or corn.

6. The application of a recombinant bacterium in regulating plant drought stress resistance, characterized in that, The recombinant bacteria contain the components described in claim 1. GhHAT18 Genes; the plants mentioned are cotton, Arabidopsis thaliana, rapeseed, wheat, rice, or corn.

7. A method for cultivating a drought-resistant transgenic plant, characterized in that, Includes the following steps: The claim 1 GhHAT18 Genes are linked to expression vectors to construct recombinant expression vectors; The recombinant expression vector was transformed into Agrobacterium to obtain recombinant Agrobacterium; By infecting plants with the recombinant Agrobacterium, drought-resistant transgenic plants were obtained.

8. The cultivation method according to claim 7, characterized in that, The expression vector is WMV067, pCAMBIA2300, pCAMBIA1301 or PBI121.

9. The cultivation method according to claim 7, characterized in that, The Agrobacterium species mentioned are GV3101, EHA105, or LBA4404.

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