Microbiome-friendly substance and microbiome-friendly cosmetic preparation

Abstract

The present invention relates to the use of specific substances as microbiome-friendly ingredients in cosmetic formulations. Furthermore, the present invention relates to a cosmetic formulation having a microbiome-friendly composition.

Description

[0001] This invention relates to the use of specific substances as microbiome-friendly ingredients in cosmetic formulations. Furthermore, this invention relates to cosmetic formulations having a microbiome-friendly composition.

[0002] This invention relates to the use of formulation ingredients (galen preparations, such as emulsifiers, emollients, rheology modifiers) that do not significantly alter the natural composition of the skin and / or scalp and / or mucous membrane microbiota and the specific microbiome associated therewith. Because the composition of the microbial community is adapted to the host and its environment, it plays a crucial role in limiting pathogen growth and maintaining the natural balance necessary to help maintain host health.

[0003] The following definition of the skin microbiome essentially refers to the report made by the Cosmetic Regulatory Cooperation International on June 28, 2022. The skin microbiome exists on the entire surface of the skin, including the mucous membranes of the oral cavity and external genitalia. The composition of the skin microbiome is dynamic, site-specific, but also varies from person to person. The skin microbiome also includes microorganisms found, for example, on the scalp, hair follicle sebaceous follicles, or sebaceous glands. It also includes microorganisms detected in the epidermis or dermis. It should be noted that the two terms "microbiota" and "microbiome" are not synonymous, as microbiota refers only to microorganisms, while microbiome also includes their functional potential. These two terms are primarily used in scientific literature, while in the public domain, they are generally used interchangeably.

[0004] The microbiome is a characteristic community of microorganisms occupying a rationally defined habitat and possessing unique physiological and chemical characteristics. The microbiome refers not only to the microorganisms involved but also to their habitats, which lead to the formation of specific ecological niches. This includes their genetic material, as well as structural molecules such as enzymes, membrane lipids, or polysaccharides.

[0005] The field of microbiome research has made significant progress in recent years. Historically, microbes found on or inside the human body have often been considered pathogens and inherently harmful to human health. With the introduction of new methods, this misconception has been proven true. It has been discovered that the microbial communities on and inside our bodies contribute to our health. Microbes inhabiting, for example, our skin, interact with each other and with their hosts. On human skin, *Staphylococcus epidermidis* can produce regulators that inhibit the growth of some pathogens; *Propionibacterium acnes* (formerly known as *Propionibacterium acnes*) can degrade triglycerides found in sebum, thereby setting free fatty acids necessary to maintain the natural skin pH and skin barrier. This further enhances the skin's natural immunity by inhibiting the growth of certain bacteria and by, for example, stimulating the expression of β-defensin 2 by the skin. This intricate interaction is further influenced by various factors such as sex, age, body location, individual susceptibility, and environment. For example, the composition of the skin microbiome adapts to its host and environment. Dysbiosis can lead to skin damage and is associated with skin conditions such as atopic dermatitis involving the pathogen Staphylococcus aureus.

[0006] The effects of topical application on, for example, the skin microbiome are being increasingly explored. The use of topical substances has the potential to influence the microbiome, which can in turn lead to dysbiosis. Currently, the focus is on how bioactive substances such as prebiotics, probiotics, or postbiotics can alter the composition of the skin microbiome. However, it is equally clear that the healthy balance of the natural microbiome is also crucial.

[0007] US2008 / 0070986 discloses the use of alkyl ether citrates for protecting and maintaining the natural balance processes that limit the number of pathogens and prevent them from becoming disease triggers.

[0008] Specchemonline.com, November / December 2021, pp. 24-26, discloses a microbiome-friendly formulation chassis. Single cosmetic ingredients and formulations have been tested in vitro (MIC test; MIC: minimum inhibitory concentration) and in vivo.

[0009] EP 3 970 802 discloses a skin care composition for promoting the skin microbiome, comprising (a) pyruvate and / or lactate with (b1) urea and / or arginine, or with (b2) caprylic / capric triglyceride, or a combination of (b1) and (b2).

[0010] A potential problem with this invention is providing ingredients for cosmetic formulations that avoid significantly altering healthy microbial communities and microbiomes. Such ingredients are hereinafter referred to as microbiome-friendly. Formulations that generally avoid significantly altering healthy microbial communities and microbiomes are also referred to as microbiome-friendly.

[0011] If the ingredients themselves are known in the prior art, but their use as microbiome-friendly ingredients in cosmetic formulations is unknown in the prior art, then this use is the solution to the potential problem of the present invention.

[0012] If microbiome friendliness is determined by MIC values, then the MIC values ​​determined for the microorganism Staphylococcus epidermidis are particularly relevant when tested in a medium of ethanol / water (1:1).

[0013] Therefore, the solution to the potential problems of the present invention, and The first subject of the present inventionThis refers to the use of a substance as a microbiome-friendly ingredient in cosmetic formulations, wherein the substance is selected from the group consisting of: cetearyl alcohol polyether-12, cetearyl alcohol (and) lecithin (and) sodium cetearyl sulfate (and) Olus oil, cetearyl glucoside (and) cetearyl alcohol, disodium cetearyl sulfosuccinate, ethyl linoleate, glyceryl oleate, glyceryl stearate (and) PEG-100 stearate, glyceryl stearate citrate, lauryl glucoside (and) polyglycerol-2 dihydroxystearate (and) glycerin, lecithin, polyglycerol-2 dihydroxystearate, potassium cetyl phosphate, sodium cetearyl sulfate, lauryl alcohol-7 citrate, glycerin, dehydroacetic acid (and) benzyl alcohol, glycerin (and) water (Aqua) (and) sodium levulinate (and) Sodium anisinate, phenoxyethanol (and) ethylhexylglycerin, phenoxyethanol (and) hydroxyacetophenone (and) octanediol (and) water, sodium benzoate, acrylate / behenyl ether-25 methacrylate copolymer, alginate, safflower gum, glucomannan, sodium polyacrylate, xanthan gum, 1,4-butanediol, butylene glycol, polyethylene glycol, triethyl citrate, conjugated linoleic acid, vegetable oil, passion fruit seed oil, dioctyl carbonate, isononyl isononanoate, cocoyl octanoate / caprylate, dioctyl ether, octyl octanoate / caprylate, shea butter (Butyrospermum) Parkii (Shea) Butter), propyl heptyl octanoate, undecane (and) tridecane, citric acid, hydrogenated vegetable glycerides, sucrose polystearate (and) cetyl palmitate, sodium stearoyl glutamate, octyl dodecanol, phenoxyethanol (and) methylparaben, polyglycerol-3 diisostearate, cetearyl alcohol, ethylparaben (and) propylene glycol, dipropyl heptyl carbonate, glycerol (and) myristate (and) cetearyl alcohol polyether-12 (and) glyceryl stearate (and) cetearyl alcohol (and) The composition includes: potassium cetyl phosphate, glycerol (and) cetyl palmitate (and) cetearyl alcohol polyether-12 (and) glyceryl stearate (and) pentyl glycol (and) sodium stearoyl glutamate (and) cetearyl alcohol polyether-20 (and) tetrahydroxypropyl ethylenediamine (and) cetearyl alcohol (and) water, cetyl hexanoate, ethylhexyl palmitate, triethylhexanoate, glyceryl cocoate, polyglycerol-10 stearate, polyglycerol-10 oleate, and cetearyl glucoside (and) cetearyl alcohol.

[0014] One embodiment of the present invention is the use of a substance as a microbiome-friendly ingredient in cosmetic formulations, wherein the substance is an emulsifier. This means that one embodiment of the invention is the use of a substance as a microbiome-friendly ingredient in cosmetic formulations, wherein the substance is selected from the group consisting of: cetearyl alcohol polyether-12, cetearyl alcohol (and) lecithin (and) sodium cetearyl sulfate (and) olus oil, cetearyl glucoside (and) cetearyl alcohol, disodium cetearyl sulfosuccinate, ethyl linoleate, glyceryl oleate, glyceryl stearate (and) PEG-100 stearate, glyceryl stearate citrate, lauryl glucoside (and) polyglycerol-2 dihydroxystearate (and) glycerin, lecithin, polyglycerol-2 dihydroxystearate, potassium cetearyl phosphate, sodium cetearyl sulfate, lauryl alcohol polyether-7 citrate, polyglycerol-10 stearate, polyglycerol-10 oleate, and cetearyl glucoside (and) cetearyl alcohol.

[0015] When the intended use is limited to emulsifiers having an MIC value of not less than 1, as described in the foregoing paragraphs, a preferred embodiment of the first subject matter of the invention is obtained. The relevance of the MIC value is explained in the experimental section of this document. An MIC value of not less than 1 essentially means that substances having such a high MIC value have a particularly favorable effect on the microbiome.

[0016] Therefore, a preferred embodiment of the first subject matter of the present invention is the use of a substance as a microbiome-friendly ingredient in cosmetic formulations, wherein the substance is selected from the group consisting of: cetearyl alcohol (and), lecithin (and), sodium cetearyl sulfate (and), olus oil, cetearyl glucoside (and), cetearyl alcohol, ethyl linoleate, glyceryl oleate, glyceryl stearate (and), PEG-100 stearate, glyceryl stearate citrate, lecithin, polyglycerol-2 dihydroxystearate, and potassium cetyl phosphate.

[0017] More preferred embodiments are obtained when the application is limited to emulsifiers having an MIC value of not less than 3.

[0018] Therefore, a more preferred embodiment of the first subject matter of the present invention is the use of a substance as a microbiome-friendly ingredient in cosmetic formulations, wherein the substance is selected from the group consisting of glyceryl stearate citrate and lecithin.

[0019] Preferably, the uses described in the preceding paragraphs are non-therapeutic uses.

[0020] Second subject of the invention This corresponds to the use described in the preceding paragraphs. It is a method for cosmetically treating the skin in a microbiome-friendly manner, which involves bringing the skin into contact with substances as defined in the preceding paragraphs.

[0021] Specifically, the second subject of the present invention is a method for cosmetically treating skin in a microbiome-friendly manner, the method comprising contacting the skin with a substance selected from the group consisting of: cetearyl alcohol polyether-12, cetearyl alcohol (and) lecithin (and) sodium cetearyl sulfate (and) olus oil, cetearyl glucoside (and) cetearyl alcohol, disodium cetearyl sulfosuccinate, ethyl linoleate, glyceryl oleate, glyceryl stearate (and) PEG-100 stearate, glyceryl stearate citrate, lauryl glucoside (and) polyglycerol-2 dihydroxystearate Fatty acid esters (and) glycerin, lecithin, polyglycerol-2 dihydroxystearate, potassium cetyl phosphate, sodium cetearyl sulfate, glycerin, dehydroacetic acid (and) benzyl alcohol, glycerin (and) water (and) sodium levulinate (and) sodium anisinate, phenoxyethanol (and) ethylhexylglycerin, phenoxyethanol (and) hydroxyacetophenone (and) octanediol (and) water, sodium benzoate, acrylate / behenyl ether-25 methacrylate copolymer, alginate, strychnine gum, glucomannan, sodium polyacrylate, xanthan gum, 1,4-butanediol, butylene glycol, polyethylene glycol, triethyl citrate, lauryl ether -7 Citrate, Conjugated Linoleic Acid, Vegetable Oil, Passion Fruit Seed Oil, Dioctyl Carbonate, Isononyl Isononyl Acid, Cocoyl Caprylate / Caprate, Dioctyl Ether, Octyl Caprylate / Caprate, Shea Butter, Propyl Heptyl Caprylate, Undecane (and) Tridecane, Citric Acid, Hydrogenated Vegetable Glyceryl Esters, Sucrose Polystearate (and) Cetyl Palmitate, Sodium Stearoyl Glutamate, Octyl Dodecanol, Phenoxyethanol (and) Methylparaben, Polyglycerol-3 Diisostearate, Cetearyl Alcohol, Ethylparaben (and) Propylene Glycol, Dipropyl Heptyl Carbonate, Glyceryl (and) Myristyl Myristate (and) cetearyl alcohol polyether-12 (and) glyceryl stearate (and) cetearyl alcohol (and) cetearyl phosphate potassium, glyceryl (and) cetearyl palmitate (and) cetearyl alcohol polyether-12 (and) glyceryl stearate (and) pentyl glycol (and) sodium stearoyl glutamate (and) cetearyl alcohol polyether-20 (and) tetrahydroxypropyl ethylenediamine (and) cetearyl alcohol (and) water, cetearyl ethylhexanoate, cetearyl palmitate, ethylhexyl palmitate, triisooctanoic acid glyceryl ester, glyceryl cocoate, polyglycerol-10 stearate, polyglycerol-10 oleate, and cetearyl glucoside (and) cetearyl alcohol.

[0022] The preferred embodiments of the methods described in the preceding paragraphs are methods relating to selected substances corresponding to the preferred uses described herein.

[0023] The third subject of this inventionIt is a substance used in methods of treating a human or animal body by means of a therapy including prevention, wherein the substance is selected from the group consisting of: cetearyl alcohol polyether-12, cetearyl alcohol (and) lecithin (and) sodium cetearyl sulfate (and) olus oil, cetearyl glucoside (and) cetearyl alcohol, disodium cetearyl sulfosuccinate, ethyl linoleate, glyceryl oleate, glyceryl stearate (and) PEG-100 stearate, glyceryl stearate citrate, lauryl glucoside (and) polyglycerol-2 dihydroxystearate (and) glycerol, lecithin ... Phospholipids, polyglycerol-2 dihydroxystearate, potassium cetyl phosphate, sodium cetearyl sulfate, glycerin, dehydroacetic acid (and) benzyl alcohol, glycerin (and) water (and) sodium levulinate (and) sodium anisinate, phenoxyethanol (and) ethylhexylglycerin, phenoxyethanol (and) hydroxyacetophenone (and) octanediol (and) water, sodium benzoate, acrylate / behenyl ether-25 methacrylate copolymer, alginate, strychnine gum, glucomannan, sodium polyacrylate, xanthan gum, 1,4-butanediol, butanediol, polyethylene glycol, triethyl citrate, lauryl ether-7 citric acid Esters, conjugated linoleic acid, vegetable oils, passion fruit seed oil, dioctyl carbonate, isononyl isononanoate, cocoyl octanoate / caprylate, dioctyl ether, octyl caprylate / caprylate, shea butter, propyl heptyl octanoate, undecane (and) tridecane, citric acid, hydrogenated vegetable glycerides, sucrose polystearate (and) cetyl palmitate, sodium stearoyl glutamate, octyl dodecanol, phenoxyethanol (and) methylparaben, polyglycerol-3 diisostearate, cetearyl alcohol, ethylparaben (and) propylene glycol, dipropyl heptyl carbonate, glycerol (and) myristyl myristate ( (and) Cetearyl alcohol polyether-12 (and) Glyceryl stearate (and) Cetearyl alcohol (and) Cetearyl phosphate potassium, Glyceryl (and) Cetearyl palmitate (and) Cetearyl alcohol polyether-12 (and) Glyceryl stearate (and) Pentyl glycol (and) Sodium stearoyl glutamate (and) Cetearyl alcohol polyether-20 (and) Tetrahydroxypropyl ethylenediamine (and) Cetearyl alcohol (and) Water, Cetearyl ethylhexanoate, Ethylhexyl palmitate, Glyceryl triisooctanoate, Glyceryl cocoate, Polyglycerol-10 stearate, Polyglycerol-10 oleate, and Cetearyl glucoside (and) Cetearyl alcohol.

[0024] The fourth subject of this inventionIt is a substance used in methods for treating or preventing diseases caused by dysbiosis of the skin microbiome, wherein the substance is selected from the group consisting of: cetearyl alcohol polyether-12, cetearyl alcohol (and) lecithin (and) sodium cetearyl sulfate (and) olus oil, cetearyl glucoside (and) cetearyl alcohol, disodium cetearyl sulfosuccinate, ethyl linoleate, glyceryl oleate, glyceryl stearate (and) PEG-100 stearate, glyceryl stearate citrate, lauryl glucoside (and) polyglycerol-2 dihydroxystearate (and) Glycerin, lecithin, polyglycerol-2 dihydroxystearate, potassium cetyl phosphate, sodium cetearyl sulfate, glycerin, dehydroacetic acid (and) benzyl alcohol, glycerin (and) water (and) sodium levulinate (and) sodium anisinate, phenoxyethanol (and) ethylhexylglycerin, phenoxyethanol (and) hydroxyacetophenone (and) octanediol (and) water, sodium benzoate, acrylate / behenyl ether-25 methacrylate copolymer, alginate, strychnine gum, glucomannan, sodium polyacrylate, xanthan gum, 1,4-butanediol, butylene glycol, polyethylene glycol, triethyl citrate, lauryl ether-7-limonene Citric acid esters, conjugated linoleic acid, vegetable oils, passion fruit seed oil, dioctyl carbonate, isononyl isononyl acid, coconut oil-caprylate / caprate, dioctyl ether, octylcaprylate / caprate, shea butter, propyl heptyl octanoate, undecane (and) tridecane, citric acid, hydrogenated vegetable glycerides, sucrose polystearate (and) cetyl palmitate, sodium stearoyl glutamate, octyl dodecanol, phenoxyethanol (and) methylparaben, polyglycerol-3 diisostearate, cetearyl alcohol, ethylparaben (and) propylene glycol, dipropyl heptyl carbonate, glycerol (and) myristyl myristate (and) cetearyl alcohol polyether-12 (and) glyceryl stearate (and) cetearyl alcohol (and) cetearyl phosphate potassium, glyceryl (and) cetearyl palmitate (and) cetearyl alcohol polyether-12 (and) glyceryl stearate (and) pentyl glycol (and) sodium stearoyl glutamate (and) cetearyl alcohol polyether-20 (and) tetrahydroxypropyl ethylenediamine (and) cetearyl alcohol (and) water, cetearyl ethylhexanoate, ethylhexyl palmitate, triisooctanoic acid glyceryl ester, glyceryl cocoate, polyglycerol-10 stearate, polyglycerol-10 oleate, and cetearyl glucoside (and) cetearyl alcohol.

[0025] The fifth subject of this invention It is a cosmetic formulation having the same composition as any of the following formulations or having a composition that deviates from the composition of the following formulations, such that the amount of the components in the cosmetic formulation deviates from the amount of the components in the following formulations by ±20%, preferably ±10%, more preferably ±5%:

[0026] Preparation 1:

[0027]

[0028] Preparation 2:

[0029]

[0030]

[0031] Preparation 3:

[0032]

[0033] Preparation 4:

[0034] INCI % by weight water Add to 100 Sodium benzoate 0.25 Butylene glycol 10.00 Disodium cetearyl sulfosuccinate 1.00 glycerin 5.00 Xanthan Gum 1.00 Polyglycerol-3 diisostearate 2.00 Cetearyl alcohol 2.00 Octyl octanoate / decanoate 5.00 dioctyl carbonate 5.00 Undecane (and)tridecane 2.00 Citric acid 1.67 Glycerin (and) water (and) sodium levulinate (and) sodium anisinate 1.00

[0035] Preparation 5:

[0036] INCI % by weight water Add to 100 Sodium benzoate 0.25 Butylene glycol 10.00 Sodium stearoyl glutamate 0.50 glycerin 5.00 Xanthan Gum 1.00 Cetearyl glucoside (and) cetearyl alcohol 4.00 Cetearyl alcohol 1.00 Octyl octanoate / decanoate 5.00 dioctyl carbonate 5.00 Undecane (and)tridecane 2.00 Citric acid 1.57 Glycerin (and) water (and) sodium levulinate (and) sodium anisinate 1.00

[0037] Preparation 6:

[0038] INCI % by weight water Add to 100 Phenoxyethanol, ethylhexylglycerin 1.00 Butylene glycol 5.00 Lauryl glucoside, polyglycerol-2 dimerized hydroxystearate, glycerin 3.50 Cetearyl alcohol 1.00 dioctyl carbonate 4.00 Shea butter 5.00 Sodium polyacrylate 1.50

[0039] Preparation 7:

[0040]

[0041] Preparation 8:

[0042]

[0043]

[0044] Preparation 9:

[0045] INCI % by weight Octyl octanoate / decanoate 25.00 Cetyl hexanoate 25.00 Ethylhexyl palmitate 25.00 Triisooctanoic acid glyceride 25.00

[0046] Another embodiment of the present invention is the non-therapeutic cosmetic use of any one of formulations 1 to 9 as a microbiome-friendly cosmetic formulation.

[0047] Another embodiment of the present invention is a method for cosmetically treating skin in a microbiome-friendly manner, the method comprising contacting the skin with any one of formulations 1 to 9.

[0048] Another embodiment of the invention is a formulation selected from the group consisting of formulations 1 to 9, for use in a method of treating a human or animal body by means of a therapy including prevention.

[0049] Another embodiment of the invention is a formulation selected from the group consisting of formulations 1 to 9, for use in a method for treating or preventing diseases caused by dysbiosis of the skin microbiome.

[0050] Because the composition of the healthy skin microbiome varies from person to person, the primary objective was to identify ingredients that would not disrupt the complex microbial community found on healthy skin. Ingredient selection was screened using a minimum inhibitory concentration (MIC) test and / or 16S rDNA / rRNA microbiome analysis of skin swabs (in vivo) collected from human volunteers with healthy skin before and 28 days after use. Based on these results, skincare formulations were developed and tested in vivo as proof-of-concept studies.

[0051] Significantly altering a healthy gut microbiome can have several negative consequences. Some of these consequences are purely cosmetic, such as skin redness and dryness. Therefore, avoiding these consequences is a cosmetic activity, not a medical treatment or prevention. Other consequences may be pathological and could lead to diseases caused by dysbiosis of the skin microbiome.

[0052] The statement “a deviation from the composition of a given formulation, such that the amount of a component of the composition deviates from the amount of a component of the given formulation by ±10%” means that any composition having, for example, 5% glycerol and 10% butanediol by weight, based on a given formulation, is a composition according to the definition given by the statement.

[0053] Example

[0054] Unless otherwise defined, % means by weight.

[0055] Test methods

[0056] Hydrophilic components, including emulsifiers and rheology modifiers, were evaluated using the minimum inhibition concentration (MIC) test. Lipophilic substances and formulations, including emollients, were tested on the skin of human volunteers (in vivo testing).

[0057] Minimum Inhibitory Concentration (MIC) Test

[0058] The minimum inhibitory concentration (MIC) test is a typical test used to measure growth inhibition caused by a substance. The MIC is defined as the lowest test concentration of the test substance at which the growth of a specific microorganism is inhibited.

[0059] In this case, a predefined dilution of the test substance was prepared in an appropriate culture medium and inoculated with the target microorganism. After incubation, the test organisms were cultured for a period of time under normal conditions that allow for microbial growth. A medium control (medium = the solvent used, such as water) was also evaluated to determine growth in the absence of the test substance. The target organisms tested were representative strains of Staphylococcus aureus (found on the skin and often a pathogen), Staphylococcus epidermidis, and Malassezia furfur. Higher MIC values ​​indicated less inhibition of growth. In the following results, the test concentration is given as a percentage of the active substance content.

[0060] In vivo testing on human skin

[0061] In vivo studies were conducted. The general procedure is as follows: At least 20 volunteers who provided informed consent and presented healthy skin were required to have a test sample applied to the palmar side of their forearm twice daily for 28 days. Volunteers were asked to avoid using topical products for 7 days prior to the start of the study and throughout the study. Each test sample was randomly applied to one of four predefined test areas. Untreated areas were used as references. Skin was treated under standardized conditions. Target areas were swabbed before sample application and 28 days after application. Swabs were stored on dry ice until genetic analysis was performed.

[0062] Genetic analysis

[0063] Primers targeting the variable regions of bacterial 16S rDNA / rRNA were used to amplify the genetic material acquired during swabbing via polymerase chain reaction (PCR). After sequencing, sequences were clustered into operational taxonomic units (OTUs) at a 97% similarity threshold, defining taxonomic levels such as phylum and genus. Alpha diversity was used as a measure of microbial richness and abundance, and the Shannon diversity index was calculated to determine biodiversity. Statistical significance was determined using the Wilcoxon signed-rank test.

[0064] Results of MIC test (MIC is expressed as a percentage by weight).

[0065]

[0066]

[0067]

[0068] These results will be interpreted as follows. For each substance, the lowest MIC value obtained determines whether the substance can be used as a microbiome-friendly cosmetic ingredient. If this lowest MIC is not lower than a threshold, the substance can be used as a microbiome-friendly cosmetic ingredient. This threshold depends on the type of substance (solvent, emulsifier, etc.) and is related to the amount of these types of substances typically used in common cosmetic formulations.

[0069] For emulsifiers, the minimum MIC should not be less than 0.05%, preferably not less than 0.08%, more preferably not less than 0.5%, more preferably not less than 1%, more preferably not less than 2%, and more preferably not less than 3%.

[0070] For wetting agents, the minimum MIC should not be less than 0.1%, preferably not less than 0.25%, preferably not less than 0.5%, preferably not less than 1%, and preferably not less than 2%.

[0071] For preservatives, the minimum MIC should not be less than 0.25%, preferably not less than 0.5%, more preferably not less than 1%, and even more preferably not less than 2%.

[0072] For rheology modifiers, the minimum MIC should not be less than 0.1%, preferably not less than 0.25%, preferably not less than 0.5%, preferably not less than 1%, and preferably not less than 2%.

[0073] For solubilizers, the minimum MIC should not be less than 1%, preferably not less than 2%, and more preferably not less than 20%.

[0074] For conjugated linoleic acid, the minimum MIC should not be lower than 1%.

[0075] Results of genetic analysis / in vivo testing

[0076] In vivo testing was conducted using the formulations listed in the table below. Each formulation and its constituent ingredients were found to be suitable as a microbiome-friendly formulation and ingredient. Therefore, any ingredient in the tested formulations can be included in the list of ingredients present in claims 1, 3, 4, and 5 of this filed patent application.

[0077]

[0078]

[0079]

[0080]

[0081]

[0082] Preparations

[0083] The following formulation is for genetic analysis / in vivo testing. Formulation number SC-DE-20-089-1

[0084]

[0085] Preparation code SC-DE-20-044-2

[0086]

[0087] Preparation code SC-DE-20-067-8

[0088]

[0089]

[0090] Preparation code SC-CN-22-VZ011302

[0091]

[0092] Preparation code SC-SC-22-VZ011102

[0093]

[0094] Preparation code SC-CN-22-VZ021001

[0095]

[0096]

[0097] Product code SC-CN-22-VZ092002

[0098]

[0099] Product code SC-CN-22-WL092002

[0100]

[0101]

[0102] Preparation code SC-CN-22-VZ101901

[0103]

Claims

1. Use of substances as microbiome-friendly ingredients in cosmetic formulations. The substance is selected from the following group: Cetearyl alcohol polyether-12, cetearyl alcohol (and) lecithin (and) sodium cetearyl alcohol sulfate (and) olus oil, cetearyl glucoside (and) cetearyl alcohol, disodium cetearyl alcohol sulfosuccinate, ethyl linoleate, glyceryl oleate, glyceryl stearate (and) PEG-100 stearate, glyceryl stearate citrate, lauryl glucoside (and) polyglycerol-2 dihydroxystearate (and) glycerin, lecithin, polyglycerol-2 dihydroxystearate, potassium cetearyl phosphate, sodium cetearyl alcohol sulfate, linoleic acid Cinnamyl ether-7 citrate, glycerin, dehydroacetic acid (and) benzyl alcohol, glycerin (and) water (and) sodium levulinate (and) sodium anisinate, phenoxyethanol (and) ethylhexylglycerin, phenoxyethanol (and) hydroxyacetophenone (and) octyl glycol (and) water, sodium benzoate, acrylate / behenyl ether-25 methacrylate copolymer, alginate, safflower gum, glucomannan, sodium polyacrylate, xanthan gum, 1,4-butanediol, butylene glycol, polyethylene glycol, triethyl citrate, conjugated linoleic acid, vegetable oil, passion fruit seed oil, dioctyl carbonate, Isonononyl isononate, coconut oil-caprylate / caprylate, dioctyl ether, octylcaprylate / caprylate, shea butter, propyl heptyl octanoate, undecane (and) tridecane, citric acid, hydrogenated vegetable glycerides, sucrose polystearate (and) cetyl palmitate, sodium stearoyl glutamate, octyl dodecanol, phenoxyethanol (and) methylparaben, polyglycerol-3 diisostearate, cetearyl alcohol, ethylparaben (and) propylene glycol, dipropyl heptyl carbonate, glycerin (and) myristyl myristate (and) cetearyl alcohol polyether-12 (and) glyceryl stearate (and) cetearyl alcohol (and) potassium cetearyl phosphate, glyceryl (and) cetearyl palmitate (and) cetearyl alcohol polyether-12 (and) glyceryl stearate (and) pentylene glycol (and) sodium stearoyl glutamate (and) cetearyl alcohol polyether-20 (and) tetrahydroxypropyl ethylenediamine (and) cetearyl alcohol (and) water, cetearyl ethylhexanoate, ethylhexyl palmitate, triisooctanoic acid glyceryl ester, glyceryl cocoate, polyglycerol-10 stearate, polyglycerol-10 oleate, and cetearyl glucoside (and) cetearyl alcohol.

2. The use of the substance according to claim 1 as a microbiome-friendly ingredient in cosmetic formulations, wherein, The substance is selected from the group consisting of: cetearyl alcohol polyether-12, cetearyl alcohol (and) lecithin (and) sodium cetearyl sulfate (and) olus oil, cetearyl glucoside (and) cetearyl alcohol, disodium cetearyl sulfosuccinate, ethyl linoleate, glyceryl oleate, glyceryl stearate (and) PEG-100 stearate, glyceryl stearate citrate, lauryl glucoside (and) polyglycerol-2 dihydroxystearate (and) glycerol, lecithin, polyglycerol-2 dihydroxystearate, potassium cetearyl phosphate, sodium cetearyl sulfate and lauryl alcohol polyether-7 citrate.

3. The use of the substance according to claim 1 as a microbiome-friendly ingredient in cosmetic formulations, wherein, The substance was selected from the group consisting of: cetearyl alcohol (and), lecithin (and), sodium cetearyl sulfate (and), olus oil, cetearyl glucoside (and), cetearyl alcohol, ethyl linoleate, glyceryl oleate, glyceryl stearate (and), PEG-100 stearate, glyceryl stearate citrate, lecithin, polyglycerol-2 dihydroxystearate and potassium cetyl phosphate.

4. The use of the substance according to claim 1 as a microbiome-friendly ingredient in cosmetic formulations, wherein, This substance is selected from the group consisting of glyceryl stearate, citrate, and lecithin.

5. A cosmetic formulation having the same composition as or a composition deviating from that of any of the following formulations, such that the amount of the components in the cosmetic formulation deviates from the amount of the components in the following formulations by ±20%, preferably ±10%, more preferably ±5%: Preparation 1: Preparation 2: Preparation 3: Preparation 4: Preparation 5: Preparation 6: Preparation 7: Preparation 8: Preparation 9:

6. The formulation according to claim 5 for non-therapeutic cosmetic use as a microbiome-friendly cosmetic formulation.

7. A method for cosmetically treating skin in a microbiome-friendly manner, the method comprising contacting the skin with the preparation according to claim 5.

8. The preparation according to claim 5, for use in a method of treating a human or animal body by means of a therapy including prevention.

9. The preparation according to claim 5, for use in a method for treating or preventing diseases caused by dysbiosis of the skin microbiome.

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