Oral care compositions for increased confidence

Abstract

The present invention relates to the use of an oral care composition comprising (1R, 2S, 5R)-N-(4-cyanomethylphenyl)-p-menthane carboxamide for increasing the level and / or duration of individual confidence.

Description

[0001] This invention relates to the use of oral care compositions comprising (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthaneformamide. In particular, this invention relates to the use of oral care compositions comprising (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthaneformamide for increasing individual self-confidence. Background of the Invention

[0003] Bad breath varies considerably throughout the day and night, and when it exceeds socially acceptable levels, it is termed bad breath or oral malodor. People with better oral health appear to have better human interactions, and therefore higher self-esteem and confidence. In consumer marketing, oral care brands with packaging claims associated with social confidence are positioned primarily by leveraging the product's ability to boost confidence by delivering core category benefits such as a fresh taste, smell, and flavor (to oneself and others); a clean, healthy mouth; and attractively white teeth. These packaging claims primarily serve as proof of confident behavior related to social situations and feelings.

[0004] Self-confidence is considered one of the most influential motivators and behavioral regulators in people's daily lives. Self-confidence is "a person's belief that they can successfully perform a specific activity, rather than a general trait that explains overall optimistic performance."

[0005] It is known that the mere thought of having bad breath is enough to affect an individual's self-confidence (McKeown L. (2003) Social relations and breath odour).

[0006] Brushing teeth alone has been shown to be sufficient to boost self-confidence (Taylor, P. et al., The Journal of Social Psychology, 2021, 182-196). However, most studies have only assessed the immediate effects of brushing on self-confidence (i.e., immediately after brushing). These studies also do not directly measure self-confidence, relying solely on its correlation with perceived expectations.

[0007] There is a need to directly link self-confidence to actual consumer goods. In recent years, methods for measuring individual self-confidence have been developed, such as the "Self-Reported Self-Confidence Scale," which is administered in questionnaire format and requires respondents to report their level of self-confidence. The Self-Reported Self-Confidence Scale is an overall self-assessment of self-confidence, requiring reflection on personal experiences and tendencies. Typically, items on the Self-Reported Self-Confidence Scale require respondents to consider their confidence in a specific task or domain and select the degree to which they agree with statements or questions related to their own behavior. Therefore, this method is particularly well-suited for measuring individual self-confidence.

[0008] Cooling agents are largely tasteless and odorless; however, they have been used to produce the cooling effect in consumer products such as toothpaste or mouthwash. Their use in consumer products can also influence consumers' emotional states, affecting, for example, mood and feelings, and evoking positive memories and feelings of well-being.

[0009] One object of the present invention is to use cooling agents, particularly (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthanecarboxamide, in oral care compositions to increase the level of individual self-confidence and increase the persistence of individual self-confidence. Invention Overview

[0011] In one aspect, the use of an oral care composition comprising (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide for increasing individual self-confidence levels is provided.

[0012] In another aspect, the use of an oral care composition comprising (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide is provided for increasing the duration of individual self-confidence.

[0013] In another aspect, an oral care composition comprising (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide is provided for use in increasing both the level and duration of an individual's self-confidence. Brief description of the attached diagram

[0015] Figure 1 The comparison shows the levels of breath freshness measured at different time points within 60 minutes after brushing with a single flavored toothpaste versus a flavored toothpaste containing 150 ppm and 250 ppm of (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide.

[0016] Figure 2 The study shows a comparison of confidence levels measured at different time points within 60 minutes after brushing with a single flavored toothpaste versus a flavored toothpaste containing 150 ppm and 250 ppm of (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide.

[0017] definition

[0018] As used in this article, “confidence” refers to the firmness or strength of one’s belief in one’s ability (“ability”; “the ability to do something successfully or effectively”).

[0019] The “level” of self-confidence involves the strength of one’s belief in one’s abilities.

[0020] The “duration” of self-confidence refers to the length of time people believe they feel any measurable level of self-confidence.

[0021] Unless otherwise stated, all percentages and ratios are by weight.

[0022] Detailed description

[0023] Preferred and / or optional features of the invention will now be set forth. Unless the context otherwise requires, any aspect of the invention may be combined with any other aspect of the invention. Unless the context otherwise requires, any preferred or optional feature of any aspect may be combined, alone or in combination, with any aspect of the invention and with any other preferred or optional feature.

[0024] The applicant was surprised and unexpected to find that the use of (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthaneformamide in oral care compositions resulted in an increase in the level, duration, or both of the level and duration of individual self-confidence.

[0025] Therefore, it is desirable to provide oral care compositions containing (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthaneformamide to increase individual self-confidence levels and the duration of time individuals feel confident.

[0026] Cooling agent

[0027] Cooling compounds, which impart a cooling sensation to the skin or mucous membranes, are well-known in the field and are widely used in a variety of products, such as food, tobacco products, beverages, dental floss, mouthwash, and toiletries.

[0028] Examples of such reagents have been disclosed, for example, in Leffingwell, JC, Perfumer & Flavorist, 2004, 39, 34-43 and Research Disclosure RD 691054, the contents of which are incorporated herein by reference.

[0029] Commercially known cooling agents are selected from N-substituted p-menthaneformamides. Particularly important compounds include N-(4-cyanomethylphenyl)-p-menthaneformamide, N-(4-aminosulfonylphenyl)-p-menthaneformamide, N-(4-cyanophenyl)-p-menthaneformamide, N-(4-acetylphenyl)-p-menthaneformamide, N-(4-hydroxymethylphenyl)-p-menthaneformamide, N-(3-hydroxy-4-methoxyphenyl)-p-menthaneformamide, or mixtures thereof. N-(4-cyanomethylphenyl)-p-menthaneformamide can be traded under the name EverCool. TM180 was purchased from Givaudan. Other important N-substituted p-menthaneformamides include menthone glycerol acetal (e.g., from Haarmann & Reimer). MGA (for sale), N-(4-cyanomethylphenyl)-p-menthaneformamide or N-(4-cyanomethylphenyl)-5-methyl-2-(1-methylethyl)cyclohexaneformamide (e.g., commercially available from Givaudan), N-(2-(pyridin-2-yl)ethyl-3-p-menthaneformamide (e.g., commercially available from Givaudan), N-(4-aminosulfonylphenyl)-p-menthaneformamide, N-(4-cyanophenyl)-p-menthaneformamide, N-(4-acetyl) N-(4-hydroxymethylphenyl)-p-menthaneformamide, N-(3-hydroxy-4-methoxyphenyl)-p-menthaneformamide, 2-isopropyl-N,2,3-trimethylbutyramide (e.g., referred to as WS-23); N-ethyl-p-menthane-3-carboxamide (e.g., referred to as WS-3); ethyl 3-(p-menthane-3-carboxamido)acetate (e.g., referred to as WS-5), menthyl lactate (e.g., available from Haarmann & Reimer) ML (commercially available), menthol-1,2-diol (e.g., available from Takasago International under Coolant Agent 10), p-menthol-3,8-diol (e.g., available from Takasago International under PMD38), isomenthol (e.g., available from Takasago International under the name...) Commercially available), (1R,2S,5R)-2-isopropyl-5-methyl-N-(2-(pyridin-2-yl)ethyl)cyclohexane-1-carboxamide (available from Givaudan under the trade name EVERCOOL190), (1-glycero-p-menthane-3-carboxylate), (ethylene glycol-p-menthane-3-carboxylate), (N-tert-butyl-p-menthane-3-carboxamide), (N-(4-ethoxyphenyl)-p-menthane-3-carboxamide), 3-(1-menthoxy)propane-1,2-diol, 3-(1-menthoxy)-2-methylpropane-1,2-diol, menthyl pyrrolidone carboxylate (for example, as...) Commercially available), (1R,3R,4S)-3-mentheptyl-3,6-dioxane-heptyl ester (e.g., available commercially from Firmenich), methoxyacetic acid (1R,2S,5R)-3-mentheptyl ester (e.g., available commercially from Firmenich), 3,6,9-trioxanedecanoic acid (1R,2S,5R)-3-mentheptyl ester (e.g., available commercially from Firmenich), 11-hydroxy-3,6,9-trioxanedecanoic acid (1R,2S,5R)-mentheptyl ester (e.g., available commercially from Firmenich), (2-hydroxyethoxy)acetic acid (1R,2S,5R)-3-menthyl ester (e.g., commercially available from Firmenich), piperazine alcohol (e.g., commercially available from Firmenich), 1-[2-hydroxyphenyl]-4-[2-nitrophenyl]-1,2,3,6-tetrahydropyrimidin-2-one), 4-methyl-3-(1-pyrrolidinyl)-2[5H]-furanone (e.g., known as Icilin or AG-3-5), menthyl lactate, menthone glycerol acetal, L-monomenthyl succinate, L-monomenthyl glutarate, 3-1-menthoxypropane-1,2-diol (e.g., known as Coolact 10), 2-1-menthoxyethanol (e.g., known as Cooltact 5), and mixtures thereof. Other non-menthol-based cooling agents are described in U.S. Patent Nos. 7,414,152, 20100086498A1, and WO2010 / 128026A2, which are incorporated herein by reference.Other cooling agents are disclosed in U.S. Patent Nos. 4,136,163 and 4,150,052 and 4,178,459 and 4,190,643 and 4,193,936 and 4,226,988, U.S. Patent Nos. 4,230,688 and 4,032,661 and 4,153,679 and 4,296,255, U.S. Patent No. 4,459,425, and U.S. Patent No. 5,009. 893, US Patent No. 5,266,592, US Patent No. 5,698,181, US Patent Nos. 5,725,865 and 5,843,466, US Patent No. 6,231,900, US Patent No. 6,277,385, US Patent Nos. 6,280,762 and 6,306,429 and 6,432,441, US Patent No. 6,455,0 US Patent Nos. 6,627,233 and 7,078,066, 6,783,783, 6,884,906, 7,030,273 and 7,090,832; US Patent Nos. 2004 / 0175489, 2004 / 0191402, 2005 / 0019445, and 2005 / 0222256 and US2005 / 0265930, US2006 / 0159819 and US2006 / 0249167; EP1689256A1; and WO2005 / 082154A1, WO2005 / 099473A1, WO2006 / 058600A1, WO2006 / 092076A2 and WO2006 / 125334A1, all of which are incorporated herein by reference.

[0030] In particular, (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthanecarboxamide (also known as EC 180) is disclosed in WO2005 / 049553, which exhibits a strong and long-lasting cooling effect, especially when used in oral care compositions such as mouthwashes or toothpaste. (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthanecarboxamide provides a long-lasting cooling sensation in the mouth, but it has no aroma (flavor) of its own and therefore does not contribute to a pleasant odor. Surprisingly, it has now been found that despite the lack of fragrance, (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthanecarboxamide enhances confidence.

[0031] Fresh breath generally contributes significantly to an individual's confidence in social interactions and situations. While (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthaneformamide does not contribute to confidence-related benefits such as a clean, healthy mouth and pleasant odor, it has been found to enhance confidence compared to flavoring agents alone, and this enhanced confidence is also prolonged.

[0032] This invention demonstrates that the use of (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthaneformamide in oral care compositions, in addition to its known cooling effect, leads to increased consumer confidence. The increased effect is related to the magnitude and duration of the effect.

[0033] Flavor components

[0034] Flavoring ingredients are incorporated into oral care compositions, such as toothpaste, mouthwash, and chewing gum, to send a signal to consumers that the product delivers a fresh and clean feeling when used. A fresh taste and smell are key indicators for consumers that their teeth, mouth, and breath will be clean when using the product.

[0035] Examples of flavoring ingredients include natural flavoring agents, artificial flavoring agents, spices, seasonings, etc. Exemplary flavoring ingredients include synthetic flavor oils and flavoring aromatics and / or oils, oleoresins, essential oils and distillates, and combinations comprising at least one of the foregoing.

[0036] Flavoring oils include spearmint oil, cinnamon oil, wintergreen oil (methyl salicylate), peppermint oil, peppermint oil, clove oil, laurel oil, fennel oil, eucalyptus oil, thyme oil, cypress oil, nutmeg oil, allspice, sage oil, nutmeg seed coat, bitter almond oil, and cassia oil; useful flavorings include artificial, natural, and synthetic fruit flavorings such as vanilla, and citrus oils including lemon, orange, lime, grapefruit, yuzu, and sudachi, and fruit flavorings including apple, pear, peach, grape, raspberry, blackberry, currant, blueberry, strawberry, cherry, plum, prune, raisin, kola nut, guarana, neroli oil, pineapple, apricot, banana, melon, apricot, cherry, tropical fruits, mango, mangosteen, pomegranate, papaya, etc.

[0037] Other exemplary flavoring agents imparted by the flavoring composition include milk flavoring agents, butter flavoring agents, cheese flavoring agents, cream flavoring agents, and yogurt flavoring agents; vanilla flavoring agents; tea or coffee flavoring agents, such as green tea flavoring agents, oolong tea flavoring agents, tea flavoring agents, cocoa flavoring agents, chocolate flavoring agents, and coffee flavoring agents; mint flavoring agents, such as peppermint flavoring agents, spearmint flavoring agents, and Japanese mint flavoring agents; spicy flavoring agents, such as asafoetida flavoring agents, ajowan flavoring agents, fennel flavoring agents, angelica flavoring agents, cumin flavoring agents, allspice flavoring agents, cinnamon flavoring agents, chamomile flavoring agents, mustard flavoring agents, cardamom flavoring agents, coriander flavoring agents, cumin flavoring agents, clove flavoring agents, pepper flavoring agents, coriander flavoring agents, savory flavoring agents, Sichuan pepper flavoring agents, and perilla flavoring agents. Flavorings, including juniper berry flavoring, ginger flavoring, star anise flavoring, horseradish flavoring, thyme flavoring, tarragon flavoring, dill flavoring, chili flavoring, nutmeg flavoring, basil flavoring, oregano flavoring, rosemary flavoring, betel leaf flavoring, and wasabi (Japanese horseradish) flavoring; nut flavorings, such as almond flavoring, hazelnut flavoring, macadamia nut flavoring, peanut flavoring, pecan flavoring, pistachio flavoring, and walnut flavoring; alcoholic flavorings, such as wine flavoring, whiskey flavoring, brandy flavoring, rum flavoring, gin flavoring, and liqueur flavoring; floral flavorings; and vegetable flavorings, such as onion flavoring, garlic flavoring, cabbage flavoring, carrot flavoring, celery flavoring, mushroom flavoring, and tomato flavoring.

[0038] Generally, any flavoring agent or food additive (including food coloring) may be used, such as those described in Leatherhead Food International Ltd.'s "Essential guide to food additives," 3rd edition, 2008, pp. 101-321 (ISBN: 978-1-905224-50-0). This publication is incorporated herein by reference.

[0039] Many types of flavoring agents are known and used in the oral care field, but flavoring compositions containing peppermint oil (such as peppermint oil and spearmint oil) are particularly popular in oral care compositions. Peppermint oils are often used to impart a refreshing connotation because they naturally contain menthol, which has physiological cooling properties. This refreshing impression is often enhanced by including additional menthol, regardless of the type of flavoring agent.

[0040] Typical peppermint flavorings may contain anethole (up to 20%, for example 5 to 15%), menthol (up to 70%, for example 20-50%), field mint (up to 70%, for example 10-30%), peppermint (up to 50%, for example 10-30%), spearmint and / or L-carvone (up to 20%, for example 5-15%), and other flavorings or aromatic components.

[0041] Typical spearmint flavorings may contain anethole (up to 20%, for example 5 to 15%), menthol (up to 50%, for example 15-30%), spearmint and / or L-carvone (up to 50%, for example 20-40%), field mint and / or peppermint (up to 50%, for example 10-30%), and other flavorings or aromatic components.

[0042] In one embodiment, the flavor component is present in the oral care composition in the range of about 0.02% by weight to about 10% by weight, preferably in the range of about 0.2% by weight to about 5.0% by weight, and more preferably in the range of about 0.3% by weight to about 3.0% by weight.

[0043] Flavoring agents are particularly important in providing pleasant breath, but WO2004 / 073669A1 indicates that flavoring agents have a relatively short oral residence time, typically less than 10 minutes, and therefore confidence associated with the fresh breath provided by flavored toothpaste alone decreases rapidly after use.

[0044] This has been confirmed by monitoring the levels of breath freshness and self-confidence reported by many participants in this study, such as... Figure 1 and 2 As shown by the dotted line, this indicates that although breath freshness levels decrease, confidence levels increase slightly 10 minutes after brushing. Thereafter, both breath freshness and confidence levels decrease significantly over a 60-minute period.

[0045] Therefore, this invention demonstrates that brushing teeth with flavored toothpaste alone is only sufficient to maintain a certain level of confidence for a short period of time (such as about 10 minutes). However, the level of confidence then begins to decline, and the value drops significantly after about 60 minutes. Figure 1 ).

[0046] In contrast, the presence of (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol in flavored toothpaste significantly increased confidence levels 10 minutes after brushing, despite providing the same level of breath freshness over the same time period. Figure 1 and Figure 2(Solid and dashed lines in the image). Confidence levels remained elevated for approximately 30 minutes after brushing, and the effect was still observed 60 minutes after brushing, although breath freshness levels steadily declined over the same time period.

[0047] In one embodiment, (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthaneformamide is used at a level of 2 to 800 ppm, optionally 20 to 500 ppm, particularly 50 to 400 ppm, more particularly 100 to 300 ppm, and even more particularly 150 to 250 ppm, based on the weight of the oral care composition.

[0048] Oral care composition

[0049] Oral care compositions, such as toothpaste or mouthwash, can be formulated in a conventional manner well known to those skilled in the art. In one embodiment, in addition to (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide, the oral care composition also contains a flavoring ingredient.

[0050] In one embodiment, the oral care composition further comprises at least one orally acceptable carrier. The orally acceptable carrier may comprise one or more compatible solid or liquid excipients or diluents suitable for topical oral application. As used herein, “compatible” means that the components of the composition can be mixed without interacting in a manner that significantly reduces stability and / or efficacy. The carrier may include common and conventional components of dental flossers, non-abrasive gels, subgingival gels, mouthwashes or rinses, oral sprays, chewing gum, lozenges, and mints. The choice of carrier is largely determined by the manner in which the composition is introduced into the oral cavity. Carrier materials used in toothpaste, dental gels, etc., include abrasive materials, foaming agents, binders, humectants, flavoring agents, and sweeteners, as disclosed, for example, in U.S. Patent No. 3,988,433. Carrier materials for biphasic dental floss formulations are disclosed in U.S. Patent Nos. 5,213,790; 5,145,666 and 5,281,410, and U.S. Patent Nos. 4,849,213 and 4,528,180. Mouthwash, rinse, or oral spray carrier materials typically include water, flavoring agents, and sweeteners, as disclosed in U.S. Patent No. 3,988,433. Tablet carrier materials typically include a candy matrix; chewing gum carrier materials include a gum base, flavoring agents, and sweeteners, as described, for example, in U.S. Patent No. 4,083,955. Pouch carrier materials typically include a pouch, flavoring agent, and sweetener. For subgingival gels used to deliver active substances into or around periodontal pockets, “subgingival gel carriers” as disclosed, for example, in U.S. Patent Nos. 5,198,220 and 5,242,910 are selected. Carriers suitable for preparing the compositions of this disclosure are well known in the art. Their selection will depend on secondary considerations such as taste, cost, and storage stability. Other suitable types of orally acceptable carrier materials or excipients are listed in WO2010 / 059289, particularly pages 17-31, which is incorporated herein by reference.

[0051] In one embodiment, the oral care composition is toothpaste.

[0052] In one implementation plan, confidence refers to confidence in a person's oral health.

[0053] The invention is further illustrated by the following non-limiting embodiments.

[0054] Measurement methods

[0055] Using the nine subscales of the modified Competitive State Anxiety Questionnaire (CSAI-2), individuals' thoughts and feelings about their confidence in their perceived oral health (e.g., bad breath) were measured.

[0056] The Competitive State Anxiety Inventory (CSAI) was originally designed by Martens et al. to examine anxiety in competitive performance (Martens, RB et al., (1990), Development and validation of the competitive state anxiety inventory-2. Competitive anxiety in sport, Human Kinetics, Chapaign, 117-190). A third subscale labeled “Confidence” was then added, characterized by positive expectations of success (CSAI-2) (Cerin, E., J. Appl. Sport psychology, 2003, 15, 223-238). CSAI-2 has become the preferred tool for measuring anxiety before intensive performances (e.g., sports or music). Arruza Gabilondo et al. (Arruza Gabilondo, J.A. et al., Revista Internacional de Medicina y Ciencias de la Actividad Fisicay del Deporte, 2012, 47, 539-556) validated a simplified version of the questionnaire using only one subscale. A recent oral care study (Dumitrescu, A.L. et.al., Int. J. Dent. Hygiene, 2012, 22-29) used a nine-item self-confidence subscale from the Competition State Anxiety Scale-2 (CSAI-2) when examining self-confidence.

[0057] The Confidence Scale consists of nine items, including five describing positive expectations ("I feel confident," "I am confident I can meet challenges," "I am confident I will perform well," "I am confident because I mentally visualize myself achieving my goals," and "I am confident I can handle pressure") and four describing general feelings of calm ("I feel calm," "I feel comfortable," "I feel safe," and "I feel mentally relaxed"). All items are rated on a 4-point scale anchored by 1 ("Not at all") and 4 ("Very much so").

[0058] Participants

[0059] This home testing study was conducted in the UK in July / August 2022. Twenty-six participants were pre-screened to ensure they did not have underlying dental problems.

[0060] Research Design and Procedures

[0061] A repeated measures design was used, with each product tested in randomized repeated trials. Three types of toothpaste were prepared using the following substances:

[0062] - Comparison: Peppermint flavoring

[0063] -EC180-150ppm: Peppermint flavoring + 150ppm EC180 (concentration in toothpaste)

[0064] -EC180-250ppm: Peppermint flavoring + 250ppm EC180 (concentration in toothpaste)

[0065] The toothpaste base formulation is shown in Table 1.

[0066] Table 1: Toothpaste Base Formulation

[0067]

[0068]

[0069] Flavor compositions also include (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide (EC180), which are shown in Table 2.

[0070] Table 2: Flavor compositions containing EC180

[0071]

[0072] Participants were given two tubes of each type of toothpaste (6 tubes in total). All products were assigned a different code and presented in white tubes to prevent color from being a confounding factor.

[0073] Participants conducted the test at home each morning according to their usual teeth cleaning time (between 7:00 AM and 10:00 AM) and were instructed to avoid using any other oral care methods besides brushing with the test product. One product was tested each day, with a one-day interval before testing the next product. A questionnaire was sent to them via an online link, and they were asked to complete it before and after teeth cleaning. Reactions before teeth cleaning were recorded as a baseline score.

[0074] Data from the test was collected using an online questionnaire developed by SurveyMonkey Audience. Participants' breath freshness and confidence were assessed at five time points: (i) baseline before brushing, (ii) 0 min after brushing, (iii) 30 min after brushing, and (iv) 60 min after brushing. A total of 47 datasets were entered for each product for analysis, and 5 incomplete datasets were removed.

[0075] Measurement

[0076] breath freshness assessment

[0077] Participants self-assessed their breath freshness using a set of three questions: (i) overall mouth cleanliness; (ii) natural mouth freshness; and (iii) a cooling sensation in the mouth. To obtain self-assessment measurements, participants were instructed to abstain from drinking water, eating, and oral hygiene practices for at least one hour before brushing and throughout the assessment period after brushing. Breath assessments recorded before brushing were categorized as baseline measurements.

[0078] confidence

[0079] Self-confidence was measured using the 9-item subscale of the Revised Competition Status Scale-2 (CSAI-2). The questionnaire used the following 4-point scale:

[0080] 1-Not at all; 2-Somewhat; 3-Moderately so; 4-Very so.

[0081] Example 1: Baseline breath freshness assessment and confidence score

[0082] Before brushing (baseline), the mean and standard deviation (SD) of the products were calculated and are shown in Tables 3 and 4 below. Two-way ANOVA showed p-values ​​greater than 0.05, indicating no significant differences in breath freshness and confidence between the products before brushing. This suggests that participants rated their breath freshness and confidence almost identically before brushing throughout the test day, prior to product use.

[0083] Table 3: Baseline (Before Brushing) Fresh Breath Awareness under Two-Factor ANOVA

[0084]

[0085] Table 4: Baseline Confidence (Before Brushing) in Two-Factor ANOVA

[0086]

[0087] Table 5 shows the baseline summary statistics of participants' confidence and breath freshness (before brushing). There were no significant differences in baseline measurements between the two questionnaires and the products.

[0088] Table 5: Baseline Measurements of Fresh Breath and Confidence

[0089]

[0090] Example 2: Δ Breath Freshness Assessment and Confidence Score

[0091] To assess the effect of changes from baseline (before brushing) to different times after brushing, the average difference between the mean score at each time point and the baseline was calculated to obtain the Δ value.

[0092] ΔBreath Freshness Assessment

[0093] Figure 1 The mean Δ value of breath freshness over time is shown for EC180-150ppm, EC180-250ppm and the control.

[0094] Two-way ANOVA (Table 6) showed that there was a statistically significant difference in the mean breath freshness score between toothpaste products (F(2,552)=10.298, p=0.000) and time (F(3,552)=33.322, p=0.000).

[0095] Post-hoc analysis of Tukey's HSD (a multiple comparison test) revealed that at all time points after brushing, the average values ​​of the toothpaste products differed significantly between the control product and the two EC180 levels. There were no significant differences in EC180 levels between the two products.

[0096] Tukey’s honest significance test (HSD) is a post-hoc test commonly used to assess the significance of differences between paired group means.

[0097] Table 6: Two-way ANOVA breath freshness assessment

[0098]

[0099] Δ Confidence Assessment

[0100] The Δ value is calculated as the change from the baseline. Figure 2 The changes in the confidence mean Δ value for EC180-150ppm, EC180-250ppm and the control are shown.

[0101] Two-way ANOVA (Table 7) showed that there was a statistically significant difference in mean self-confidence scores between toothpaste products (F(2,552) = 37.465, p = 0.000) and time (F(3,552) = 4.120, p = 0.007).

[0102] Post-hoc analysis, Tukey's HSD (test for multiple comparisons) found that the mean values ​​of the toothpaste products were significantly different between the control product and the EC180 level of 250 ppm at time 30 minutes (p = 0.071, 90% CI = [-0.576, -0.020]) and time 60 minutes (p = 0.009, 90% CI = [-0.642, -0.119]). There were no statistically significant differences between the toothpaste products at times 0 and 10 minutes.

[0103] Table 7: Two-way ANOVA Confidence Assessment

[0104]

[0105] Therefore, it is clear that although breath freshness begins to decrease significantly after brushing with toothpaste containing EC180 for 10 minutes (reaching about 60% of the initial level after brushing for 60 minutes), a person's confidence remains at a perceptible level (greater than about 80%) even after brushing for 60 minutes, compared to the level immediately obtained after brushing with toothpaste containing EC180.

[0106] It was also observed that breath freshness increased only minimally for up to 10 minutes after brushing compared to immediately after brushing (0 min), while when using toothpaste containing EC180, a significant increase in confidence levels was observed 10 minutes after brushing compared to immediately after brushing (0 min).

Claims

1. The use of an oral care composition containing (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide for increasing individual self-confidence levels.

2. The use of oral care compositions containing (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide for increasing the duration of individual self-confidence.

3. The use of oral care compositions containing (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthol carboxamide for increasing the level and duration of individual self-confidence.

4. The use according to any one of claims 1 to 3, wherein the individual confidence is confidence in their oral health.

5. The use according to any one of the preceding claims, wherein the oral care composition further comprises at least one flavoring ingredient.

6. The use according to any one of the preceding claims, wherein the at least one flavor ingredient is selected from spearmint oil, cinnamon oil, wintergreen oil, peppermint oil, peppermint oil, clove oil, laurel oil, fennel oil, eucalyptus oil, thyme oil, cypress oil, nutmeg oil, allspice, sage oil, nutmeg seed coat, bitter almond oil, and cinnamon oil; artificial, natural, and synthetic fruit flavorings, such as vanilla, and citrus oils, such as lemon, orange, lime, grapefruit, yuzu, and saffron, and fruit flavorings, such as apple, pear, peach, grape, raspberry, blackberry, currant, blueberry, strawberry, cherry, plum, prune, raisin, kola nut, guarana, neroli oil, pineapple, apricot, banana, melon, apricot, cherry, tropical fruit, mango, mangosteen, pomegranate, papaya, and mixtures thereof, optionally wherein the at least one flavor ingredient is peppermint oil, spearmint oil, or a combination thereof.

7. Use according to any one of the preceding claims, wherein (1R,2S,5R)-N-(4-cyanomethylphenyl)-p-menthaneformamide is used at a level of 2 to 800 ppm, optionally 20 to 500 ppm, particularly 50 to 400 ppm, more particularly 100 to 300 ppm, and even more particularly 150 to 250 ppm, based on the weight of the oral care composition.

8. The use according to any one of the preceding claims, wherein the oral care composition is toothpaste or mouthwash, optionally wherein the oral care composition is toothpaste.

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