Method for cleaning seeds of Berchemia lanceolata
By removing the pericarp from the seeds of the spotted vine and sealing them for 0.5-3 days, and then vigorously cleaning the pulp, the problem of decreased seed vigor was solved, resulting in pure seeds with high germination rate and high vigor, and simplifying the seed cleaning process.
Patent Information
- Application Number
- CN202511401550.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-28
- Publication Date
- 2025-11-14
AI Technical Summary
In the existing technology, there are no reported methods for cleaning seeds of *Gnaphalium affine*, which leads to decreased seed vigor and low germination rate, hindering the promotion and utilization of *Gnaphalium affine*.
After removing the pericarp of the spotted vine, the seeds with pulp are sealed for 0.5-3 days, and then the pulp is cleaned with a force of 2-8N to obtain pure seeds.
It achieves 100% germination rate and high viability of pure seeds, is easy to operate, and is suitable for cleaning and storing seeds of *Vitis thunbergii*.
Smart Images
Figure CN120937573A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of plant seed cleaning technology, specifically relating to a method for cleaning seeds of *Vitis vinifera*. Background Technology
[0002] Stixis suaveolens (Roxb.) Pierre, a large woody vine with fragrant flowers, is cultivated for ornamental purposes; its young leaves can be used as a tea substitute; and its fruit is edible (as recorded in Hainan). It grows at altitudes of 1,500 meters and is a common vine found in thickets and sparse forests. It has high economic value, but has not yet been artificially introduced and cultivated. While Stixis suaveolens has significant economic value, research on its seed biology characteristics, including seed cleaning, germination, and storage, has been lacking to date, hindering its research and utilization. Summary of the Invention
[0003] In view of this, and in order to overcome the aforementioned shortcomings of the existing technology, the purpose of this invention is to provide a method for cleaning seeds of *Vitis vinifera*. This method not only improves the vigor of pure seeds, resulting in highly vigorous and pure seeds, but it is also simple and easy to implement. Furthermore, it maintains high seed vigor, achieving a 100% germination rate, and thus has high potential for widespread application. This method provides a foundation for the promotion and utilization of *Vitis vinifera* and offers a feasible technical solution for further enhancing its economic value.
[0004] To achieve the above-mentioned objectives of the present invention, the present invention provides the following technical solution:
[0005] A method for cleaning the fruit of the spotted vine to obtain pure seeds, the method comprising the following steps:
[0006] 1) Take the fruit of the spotted vine, remove the pericarp, and obtain seeds with pulp;
[0007] 2) Seal the obtained seeds with pulp and let them sit for 0.5-3 days;
[0008] 3) Use 2-8N of force to clean the sealed seeds to remove the pulp and obtain pure seeds.
[0009] According to the method, the fruit of the spotted vine in step 1) is a mature and plump fruit of the spotted vine. Attached Figure Description
[0010] Figure 1 This is a schematic diagram of the technical route for the method of cleaning seeds of *Vitis vinifera* according to the present invention. Detailed Implementation
[0011] To further illustrate the present invention, the method for cleaning seeds of *Vitis vinifera* provided by the present invention will be described in detail below with reference to the accompanying drawings and comparative examples and embodiments. However, these descriptions should not be construed as limiting the scope of protection of the present invention.
[0012] Comparative Example 1
[0013] Mature and plump fruits of *Stixis suaveolens* (Roxb.) Pierre were collected from Malipo County, Yunnan Province, and then transported back to the Germplasm Conservation Center of the Southwest China Wild Species Germplasm Bank, Kunming Institute of Botany, for seed cleaning and germination experiments.
[0014] Five fruits of the *Ilex speciosa* were taken, and the pericarp was removed using pruning shears to obtain seeds with pulp. These seeds were then placed on a sieve with a rubber stopper and rubbed under running water with a force of 2–8 N to remove the pulp, resulting in cleaned seeds. The cleaned seeds were sown on 1% agar medium and placed in a germination experiment at 3000 lux light intensity for 12 hours, followed by 12 hours of darkness at 20℃. The germination rate was recorded and shown in Table 1.
[0015] Comparative Example 2
[0016] Five mature, plump fruits of *Stixis suaveolens* (Roxb.) Pierre were taken, and the pericarp was removed using pruning shears to obtain seeds with pulp. These seeds were sealed in a plastic bag and left at room temperature for 4 days. Then, the seeds were placed on a sieve with a rubber stopper under running water and rubbed with a force of 2–8 N to remove the pulp, resulting in cleaned seeds. The cleaned seeds were sown on 1% agar medium and placed under 3000 lux light for 12 hours, followed by 12 hours of darkness at 20°C for germination experiments. The germination rate was recorded. Germination rates are shown in Table 1.
[0017] Comparative Example 3
[0018] Five mature, plump fruits of *Stixis suaveolens* (Roxb.) Pierre were taken, and the pericarp was removed using pruning shears to obtain seeds with pulp. These seeds were sealed in a plastic bag and left at room temperature for 5 days. Then, the seeds were placed on a sieve with a rubber stopper under running water and rubbed with a force of 2–8 N to remove the pulp, resulting in cleaned seeds. The cleaned seeds were sown on 1% agar medium and placed under 3000 lux light for 12 hours, followed by 12 hours of darkness at 20°C for germination experiments. The germination rate was recorded. Germination rates are shown in Table 1.
[0019] Comparative Example 4
[0020] Five mature, plump fruits of *Stixis suaveolens* (Roxb.) Pierre were taken, and the pericarp was removed using pruning shears to obtain seeds with pulp. These seeds were sealed in a plastic bag and left at room temperature for 6 days. Then, the seeds were placed on a sieve with a rubber stopper under running water and rubbed with a force of 2–8 N to remove the pulp, resulting in cleaned seeds. The cleaned seeds were sown on 1% agar medium and placed under 3000 lux light for 12 hours, followed by 12 hours of darkness at 20°C for germination experiments. The germination rate was recorded. Germination rates are shown in Table 1.
[0021] Example 1
[0022] Five mature, plump fruits of *Stixis suaveolens* (Roxb.) Pierre were taken, and the pericarp was removed using pruning shears to obtain seeds with pulp. The seeds were sealed in a plastic bag and left at room temperature for 12-14 hours. Then, the seeds were placed on a sieve with a rubber stopper under running water and rubbed with a force of 2-8 N to remove the pulp, resulting in cleaned seeds. The cleaned seeds were sown on 1% agar medium and placed under 3000 lux light for 12 hours, followed by 12 hours of darkness at 20℃ for germination experiments. The germination rate was recorded. Germination rates are shown in Table 1.
[0023] Example 2
[0024] Five mature, plump fruits of *Stixis suaveolens* (Roxb.) Pierre were taken, and the pericarp was removed using pruning shears to obtain seeds with pulp. The seeds were sealed in a plastic bag and left at room temperature for one day. Then, the seeds were placed on a sieve with a rubber stopper under running water and rubbed with a force of 2–8 N to remove the pulp, resulting in cleaned seeds. The cleaned seeds were sown on 1% agar medium and placed under 3000 lux light for 12 hours, followed by 12 hours of darkness at 20°C for germination experiments. The germination rate was recorded. Germination rates are shown in Table 1.
[0025] Example 3
[0026] Five mature, plump fruits of *Stixis suaveolens* (Roxb.) Pierre were taken, and the pericarp was removed using pruning shears to obtain seeds with pulp. The seeds were sealed in a plastic bag and left at room temperature for two days. Then, the seeds were placed on a sieve with a rubber stopper under running water and rubbed with a force of 2–8 N to remove the pulp, resulting in cleaned seeds. The cleaned seeds were sown on 1% agar medium and placed under 3000 lux light for 12 hours, followed by 12 hours of darkness at 20°C for germination experiments. The germination rate was recorded. Germination rates are shown in Table 1.
[0027] Example 4
[0028] Five mature, plump fruits of *Stixis suaveolens* (Roxb.) Pierre were taken, and the pericarp was removed using pruning shears to obtain seeds with pulp. These seeds were sealed in a plastic bag and left at room temperature for 3 days. Then, the seeds were placed on a sieve with a rubber stopper under running water and rubbed with a force of 2–8 N to remove the pulp, resulting in cleaned seeds. The cleaned seeds were sown on 1% agar medium and placed under 3000 lux light for 12 hours, followed by 12 hours of darkness at 20°C for germination experiments. The germination rate was recorded. Germination rates are shown in Table 1.
[0029] Table 1. Germination rate statistics of seeds after cleaning in the examples and comparative examples.
[0030]
[0031] As shown in Table 1, Comparative Example 1 took a long time, and the high pulp retention rate (100%) was not conducive to later preservation, easily leading to mold growth and shortening seed lifespan. Comparative Example 2 showed no significant difference in germination rate compared to the Example, but the proportion of normal seedlings decreased significantly. The germination rate and proportion of normal seedlings in Comparative Examples 3-4 were significantly lower than those in the Example, but not significantly different from Comparative Example 1. This indicates that the prolonged sealing time in Comparative Examples 3-4 reduced seed viability, making them unsuitable for cleaning *Vitis vinifera* seeds. In conclusion, the preferred embodiment of this invention is to remove the pericarp of *Vitis vinifera* to obtain seeds with pulp; seal the obtained seeds with pulp and leave them for 0.5-3 days; then clean the sealed seeds with 2-8N of force to remove the pulp and obtain pure seeds. Using this method, the germination rate of *Vitis vinifera* seeds can reach 100%, significantly improving the germination rate and the proportion of normal seedlings, and the cleaning method is simple. The cleaning method in the Examples can significantly improve the germination rate of *Vitis vinifera* seeds, obtaining highly vigorous pure seeds, and is simple to operate.
[0032] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Anyone skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should not be determined by the scope defined in the embodiments.
Claims
1. A method for cleaning the fruits of the spotted vine to obtain pure seeds, characterized in that, The method includes the following steps: (1) Take the fruit of the spotted vine, remove the pericarp, and obtain seeds with pulp; (2) Seal the obtained seeds with pulp and leave them for 0.5-3 days; (3) Clean the sealed seeds with a force of 2-8N to remove the pulp and obtain pure seeds.
2. The method according to claim 1, characterized in that, The fruit of the spotted vine mentioned in step 1) refers to the mature and plump fruit of the spotted vine.