Sampler for collecting biomolecules from the surface of biological tissues

Abstract

This invention relates to a sampler for collecting biomolecules from the surface of biological tissues, comprising: a main body equipped with a power device, the sampler rotating under the drive of the power device; and sampling units, each equipped with a braking structure and connected to the main body via the braking structure. The braking structure is configured as follows: when the number of braking structures is ≤2, the braking structure is an automatic adjustment component used to automatically adjust the position of the sampling units to adjust the sampling radius; when the number of braking structures is >2, the braking structure is a mechanically adjustable structure, pre-setting and fixing the position of the sampling units before use to pre-set and fix the sampling radius. It features adjustable resolution and soft contact characteristics, reduces tissue disturbance through non-embedded biomolecule sampling technology, and, combined with a pre-planned and comprehensive sampling motion path design, can complete full tissue characterization and ultimately achieve biomolecule collection.

Description

Technical Field

[0001] This invention relates to the field of biological sampling technology, specifically, to a sampler for collecting biomolecules from the surface of biological tissues. Background Technology

[0002] As molecular biology, pathology, and precision medicine research delve deeper into the microscopic scale, in-situ biomolecule collection technology has become a crucial link in elucidating the mechanisms of life activities and pathological diagnosis. Against the backdrop of rapid development in minimally invasive diagnostic and treatment technologies, the need for dynamic monitoring of biomolecules on the surfaces of biological tissues (such as the epidermis of skin, organ mucous membranes, and wound repair) is increasingly urgent. Samplers for collecting biomolecules from biological tissue surfaces have been widely used, but the following problems still exist:

[0003] 1. Decreased molecular capture efficiency due to insufficient surface adaptability: Traditional samplers have difficulty effectively conforming to the complex curved or irregular surfaces of biological tissues (such as skin folds, organ mucosa, and wounds), resulting in insufficient or uneven contact between the sampling probe and the tissue;

[0004] 2. Limited spatial resolution of sampling sites: Existing technologies have difficulty in flexibly and accurately controlling the location and density of sampling points, especially at the microscale or when dense or sparse sampling of specific areas (such as around an implant) is required, making it impossible to construct high-resolution spatial distribution maps.

[0005] 3. Poor operational compatibility: Existing samplers may have complex structures and cumbersome operations, making them difficult to integrate into minimally invasive surgery or diagnostic equipment, or causing unnecessary interference to tissues during operation.

[0006] Existing samplers often face technical bottlenecks when dealing with complex tissue interfaces, such as insufficient surface adaptability leading to decreased molecular capture efficiency, limited spatial resolution of sampling sites, and poor operational compatibility. These technical limitations severely restrict the accuracy of dynamic surface molecular monitoring and hinder the possibility of real-time detection and analysis in integrated diagnostic and therapeutic scenarios. Summary of the Invention

[0007] The purpose of this invention is to provide a sampler for collecting biomolecules from the surface of biological tissues. It features adjustable resolution and soft contact characteristics. By using non-embedded biomolecule sampling technology, it reduces disturbance to the tissue. Combined with a pre-planned and complete sampling motion path design, it can complete the full spectrum characterization of the tissue and ultimately achieve biomolecule collection.

[0008] The object of this invention is achieved as follows: a sampler for collecting biomolecules from the surface of biological tissues, comprising:

[0009] The main body is equipped with a power device, and the main body is connected to the power device and rotates based on a vertical central axis under the drive of the power device.

[0010] At least one sampling unit, the sampling unit having a sampling end, wherein the sampling end of the sampling unit rotates around the central axis of the main body when in use, and each sampling unit is equipped with a braking structure and is connected to the main body through the braking structure;

[0011] The sampling radius is defined as the distance between the sampling end and the central axis, and the braking structure is configured as follows:

[0012] When the number of braking structures / sampling units is ≤2, the braking structure is set as an automatic adjustment component and used to automatically adjust the position of the sampling units to adjust the sampling radius;

[0013] When the number of braking structures / sampling units is greater than 2, the braking structure is set as a mechanically adjustable structure. Before use, the position of the sampling unit is preset and fixed by the braking structure to preset and fix the sampling radius.

[0014] Furthermore, when the number of the braking structure / sampling unit is greater than 2, the sampler is defined as a fixed sampler. The braking structure includes positioning holes on the main body. The number of positioning holes is the same as the number of sampling units and they correspond one-to-one in position. Each sampling unit can be adjusted up and down to fit into the corresponding positioning hole.

[0015] Furthermore, the surface of the sampling unit is provided with at least two limiting protrusions spaced apart from top to bottom, and each positioning hole is provided with a positioning groove with its opening facing upward and communicating with it. When the height position of the sampling unit is locked, one of the limiting protrusions can be detachably inserted into the positioning groove.

[0016] Furthermore, each positioning hole has a vertically extending strip hole on one side, which accommodates the limiting protrusion to move through when the height of the sampling unit is adjusted.

[0017] Furthermore, the top of the main body is provided with a boss, and the boss is provided with a connecting groove for transmission connection with the rotary power unit.

[0018] Furthermore, the sampling unit is arranged as follows: it includes a central sampling unit located on a vertical central axis, and two sets of peripheral sampling units. The two sets of peripheral sampling units are staggered vertically and have different sampling radii. The central sampling unit and the peripheral sampling units are distributed in a three-dimensional manner to match the entire surface of the target tissue.

[0019] Fixed samplers have the following advantages: with the structure of a double-layer preset sampling radius + a central sampling unit, efficient synchronous sampling of three independent regions (such as the two rings and one center in the pig brain experiment) can be completed in a single operation, avoiding multiple surgical interventions.

[0020] When the number of braking structures / sampling units is 2, the sampler is defined as a variable sampler, including:

[0021] A rotary motor, which is connected to the main body to drive the main body to rotate;

[0022] An automatic lifting component is installed in the middle of the main body;

[0023] A braking platform, which is connected to an automatic lifting component;

[0024] A pair of support arms are distributed opposite each other on the left and right. The upper ends of the two sampling units have sliders that slide horizontally with the main body to form two braking structures. The upper ends of the two support arms are rotatably connected to the sliders of the two sampling units respectively, and the lower ends of the two support arms are rotatably connected to the left and right sides of the braking platform respectively. Driven by the automatic lifting component, the braking platform automatically lifts and lowers to drive the two support arms and the two sampling units to work together to automatically adjust the sampling radius.

[0025] Furthermore, the braking structure includes a guide rail for guiding the motion, the slider at the upper end of the sampling unit slides horizontally with the motion rail, and a limiting seat for limiting the position of the slider of the sampling unit is installed at the end of the motion rail.

[0026] Furthermore, the automatic lifting component is configured as a lead screw motor, the output end of the lead screw motor is configured as a lead screw, and the lead screw of the lead screw motor is threadedly connected to the brake platform to drive the brake platform to lift.

[0027] The variable sampler has the following advantages: it can achieve real-time continuous radius adjustment (0.1-10mm) during the operation through the linkage of the lead screw motor and the slide rail linkage mechanism, and combined with the programmable rotation angle of the stepper motor (30°-90°) and the droplet size coordinated control (0.6±0.1mm to 1±0.1mm), it can achieve adaptive high-resolution sampling at any position on the entire surface. Attached Figure Description

[0028] Figure 1 This is a perspective view of Embodiment 1 of the present invention.

[0029] Figure 2 This is a first side view of Embodiment 1 of the present invention.

[0030] Figure 3 This is a second side view of Embodiment 1 of the present invention.

[0031] Figure 4This is a schematic diagram of the sampling unit according to Embodiment 1 of the present invention.

[0032] Figure 5 This is the assembly drawing of Embodiment 2 of the present invention.

[0033] Figure 6 This is a usage state diagram of Embodiment 2 of the present invention.

[0034] Explanation of reference numerals in the attached figures:

[0035] 11-Main body base; 11a-Positioning hole; 11b-Strip hole; 11c-Positioning groove; 11d-Connecting groove; 12-Sampling main arm; 12a-Limiting protrusion; 13-Fluid tube; 14-Sampling needle;

[0036] 21-Rotary motor; 22-Screw motor; 23-Motion slide rail; 24-Support arm; 25-Sampling arm; 26-Brake table; 27-Limit seat. Detailed Implementation

[0037] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] To address the technical problems existing in the prior art, a sampler for collecting biomolecules from the surface of biological tissues is proposed, the core of which includes:

[0039] The main body is equipped with a power unit, and the main body is connected to the power unit (servo stepper motor) and rotates based on a vertical central axis under the drive of the power unit;

[0040] At least one sampling unit, the sampling unit having a sampling end, the sampling end of the sampling unit rotates around the central axis of the main body when in use, each sampling unit is equipped with a braking structure and is connected to the main body through the braking structure;

[0041] The sampling radius is defined as the distance between the sampling end and the central axis, and the braking structure is set as follows:

[0042] When the number of braking structures / sampling units is ≤2, the braking structure is set as an automatic adjustment component and used to automatically adjust the position of the sampling unit in order to automatically adjust the sampling radius. It is set as a radius variable sampler. When the entire sampler is working, it can automatically adjust the sampling radius and rotate as a whole in order to cover the entire sampling circle. It can adjust its own radius by movement to form a suitable structure.

[0043] When the number of braking structures / sampling units is greater than 2, the braking structure is set as a mechanically adjustable structure. Before use, the position of the sampling unit is preset and fixed through the braking structure to preset and fix the sampling radius. That is, the sampling radius is adjusted and locked by mechanical adjustment, and it is set as a radius-fixed sampler. The entire sampler only needs to rotate as a whole when working. The layout and position of the sampling units can be preset in advance according to the size of the sampling surface to complete the sampling covering the entire surface.

[0044] Based on this core idea, two preferred embodiments are proposed; it is worth noting that the embodiments are not limited to the following two.

[0045] Example 1

[0046] like Figure 1-4 As shown, for cases where the number of braking structures / sampling units is greater than 2, the sampler is defined as a fixed sampler. The braking structure includes positioning holes 11a provided on the main body 11. The number of positioning holes 11a is the same as the number of sampling units, and they correspond one-to-one in position. Each sampling unit can be adjusted up and down to fit into the corresponding positioning hole 11a.

[0047] like Figure 1 , 4 As shown, the surface of the sampling unit is provided with at least two limiting protrusions 12a spaced apart from top to bottom. Each positioning hole 11a is provided with a positioning groove 11c with its opening facing upward and communicating with it. When locking the height position of the sampling unit, one of the limiting protrusions 12a can be detachably inserted into the positioning groove 11c. The height of the sampling unit can be adjusted by switching the limiting protrusions 12a. Each positioning hole 11a has a vertically penetrating strip hole 11b on one side.

[0048] The top of the main body is provided with a boss, and the boss is provided with a connecting groove 11d that is connected to the rotating power unit for transmission, so as to transmit power to make the entire sampler rotate.

[0049] In Example 1, the sampling unit includes the following parts:

[0050] Sampling main arm 12;

[0051] A fluid tube 13 is provided in the sampling main arm 12, and the fluid tube 13 protrudes downward from the lower end of the sampling main arm 12;

[0052] A sampling needle 14 is provided in the fluid tube 13, and the sampling needle 14 protrudes downward from the lower end of the fluid tube 13.

[0053] In addition, such as Figure 4As shown, there is a long strip-shaped opening on the side of the sampling main arm 12. On the one hand, if the fluid tube 13 is made of a material with high friction, the strip-shaped opening can reduce friction during the loading of the fluid tube 13. On the other hand, it also enables the sampling main arm 12 to be compatible with fluid tubes 13 of different diameters.

[0054] The preferred layout of the sampling units is as follows:

[0055] It includes a central sampling unit located on the vertical central axis, and two sets of peripheral sampling units (equivalent to two layers of peripheral sampling units). The two sets of peripheral sampling units are staggered vertically and have different sampling radii. The central sampling unit is used to sample the central part of the tissue. The central sampling unit and the peripheral sampling units are distributed in a three-dimensional manner to match the entire surface of the target tissue.

[0056] As described above, the sampler is primarily a double-layer structure, with each layer capable of assembling two symmetrical sampling arms 12 (the number can be increased). The sampling radius is pre-designed based on the surface of the biological sample to be collected, with the upper and lower outer sampling units each having a different sampling radius. After pre-setting the sampling radius, the corresponding sampling arms 12 are assembled, and the fluid tube 13 and sampling needle 14 are also assembled within the sampling arms 12, thus enabling sampling at two different radii. Finally, combined with the fluid tube 13 at the center of the main body 11, surface samples of three tissues with different radii are collected. By reasonably adjusting the sampling radius and the size of the sampling droplets, full-surface sampling of the tissue surface can be achieved.

[0057] Example 2

[0058] like Figure 5-6 The example shown addresses the case where the number of braking structures / sampling units is ≤2; the example uses the case where the number of braking structures / sampling units is =2. For ease of explanation, the main body of the sampling unit in Embodiment 2 is defined as sampling arm 25.

[0059] The sampler includes:

[0060] A rotary motor 21 is connected to the main body to drive the main body to rotate;

[0061] An automatic lifting component is installed in the middle of the main body;

[0062] Braking platform 26, which is connected to automatic lifting components;

[0063] A pair of support arms 24 are distributed opposite to each other on the left and right. The upper ends of the two sampling units have sliders that slide horizontally with the main body to form two braking structures (that is, each braking structure is a combination of slider and track, and the two braking structures are connected by the braking platform 26 and the support arms 24 to form a linkage mechanism). The upper ends of the two support arms 24 are rotatably connected to the sliders of the two sampling units respectively, and the lower ends of the two support arms 24 are rotatably connected to the left and right sides of the braking platform 26 respectively. Under the drive of the automatic lifting component, the braking platform 26 automatically lifts and lowers to drive the two support arms 24 and the two sampling units to link together and automatically adjust the sampling radius.

[0064] The slider at the upper end of the sampling unit slides horizontally with the motion slide rail 23 to guide the motion slide rail 23. The end of the motion slide rail 23 is equipped with a limiting seat 27 to limit the position of the slider of the sampling unit.

[0065] The automatic lifting component is a lead screw motor 22, the output end of which is a lead screw. The lead screw of the lead screw motor 22 is threadedly connected to the brake platform 26 to drive the brake platform 26 to lift. The brake platform 26, the two support arms 24, and the two sampling units form a linkage mechanism. While the brake platform 26 is automatically lifting, it drives the two sampling units through the two support arms 24 to drive the two sampling units to slide relative to each other, thereby automatically adjusting the sampling radius. Because the brake platform 26 is driven to lift by a lead screw, the relative sliding of the two sampling units can be kept in a precise state, thereby accurately and automatically adjusting the sampling radius.

[0066] Driven by the rotary motor 21, the entire sampler rotates, enabling it to collect all sample points within a defined radius of the circumference. The sampling radius can be adjusted at any time to meet the complex surface sampling requirements of biological samples.

[0067] During the actual sampling process, the entire sampler rotates under the control of the rotary motor 21, enabling the sampler to collect all sample points within the currently determined radius. After completing the sampling of that radius, the sampling radius is adjusted by the movement of the lead screw motor 22. Then, the sampler continues to rotate to complete the sampling operation of the current radius. This process is repeated until the surface sampling of biological samples of any radius is completed.

[0068] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "front," and "rear," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention. In this invention, it should also be noted that the terms "installation" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, an integrally formed connection, a mechanical connection, or an indirect connection through intermediate connecting parts. The specific meaning of the terms in this utility model can be understood according to the specific circumstances.

[0069] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention, and no reference numerals in the claims should be construed as limiting the scope of the claims.

[0070] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A sampler for collecting biomolecules from the surface of biological tissues, characterized in that, include: The main body is equipped with a power device, and the main body is connected to the power device and rotates based on a vertical central axis under the drive of the power device. At least one sampling unit, the sampling unit having a sampling end, wherein the sampling end of the sampling unit rotates around the central axis of the main body when in use, and each sampling unit is equipped with a braking structure and is connected to the main body through the braking structure; The sampling radius is defined as the distance between the sampling end and the central axis, and the braking structure is configured as follows: When the number of braking structures / sampling units is ≤2, the braking structure is set as an automatic adjustment component and used to automatically adjust the position of the sampling units to adjust the sampling radius; When the number of braking structures / sampling units is greater than 2, the braking structure is set as a mechanically adjustable structure. Before use, the position of the sampling unit is preset and fixed by the braking structure to preset and fix the sampling radius.

2. A sampler for collecting biomolecules from the surface of biological tissues according to claim 1, characterized in that, When the number of braking structures / sampling units is 2, the sampler includes: A rotary motor (21) is connected to the main body to drive the main body to rotate; An automatic lifting component is installed in the middle of the main body; Braking platform (26), which is connected to the automatic lifting component; A pair of support arms (24) are distributed opposite to each other on the left and right. The upper ends of the two sampling units have sliders that slide horizontally with the main body to form two braking structures. The upper ends of the two support arms (24) are rotatably connected to the sliders of the two sampling units respectively. The lower ends of the two support arms (24) are rotatably connected to the left and right sides of the braking platform (26) respectively. Under the drive of the automatic lifting component, the braking platform (26) automatically lifts and lowers to drive the two support arms (24) and the two sampling units to work together to automatically adjust the sampling radius.

3. A sampler for collecting biomolecules from the surface of biological tissues according to claim 2, characterized in that, The braking structure includes a guide rail (23) for guiding the motion, the slider at the upper end of the sampling unit is horizontally slidably engaged with the motion rail (23), and a limiting seat (27) for limiting the position of the slider of the sampling unit is installed at the end of the motion rail (23).

4. A sampler for collecting biomolecules from the surface of biological tissues according to claim 2, characterized in that, The automatic lifting component is a lead screw motor (22), the output end of the lead screw motor (22) is a lead screw, and the lead screw of the lead screw motor (22) is threadedly connected to the brake platform (26) to drive the brake platform (26) to lift.

5. A sampler for collecting biomolecules from the surface of biological tissues according to claim 1, characterized in that, When the number of the braking structure / sampling unit is greater than 2, the braking structure includes positioning holes (11a) provided on the main body. The number of positioning holes (11a) is the same as the number of sampling units, and they correspond one-to-one in position. Each sampling unit can be adjusted up and down to fit into the corresponding positioning hole (11a).

6. A sampler for collecting biomolecules from the surface of biological tissues according to claim 5, characterized in that, The surface of the sampling unit is provided with at least two limiting protrusions (12a) spaced apart from top to bottom. Each positioning hole (11a) is provided with a positioning groove (11c) with its opening facing upward and communicating with it. When the height position of the sampling unit is locked, one of the limiting protrusions (12a) can be detachably inserted into the positioning groove (11c).

7. A sampler for collecting biomolecules from the surface of biological tissues according to claim 6, characterized in that, Each positioning hole (11a) has a vertically penetrating strip hole (11b) on one side, through which the limiting protrusion (12a) moves when the height of the sampling unit is adjusted.

8. A sampler for collecting biomolecules from the surface of biological tissues according to claim 5, characterized in that, The top of the main body is provided with a boss, and the boss is provided with a connecting groove (11d) that is connected to the rotary power unit for transmission.

9. A sampler for collecting biomolecules from the surface of biological tissues according to claim 5, characterized in that, The sampling unit is arranged as follows: it includes a central sampling unit located on the vertical central axis, and two sets of peripheral sampling units. The two sets of peripheral sampling units are staggered vertically and have different sampling radii. The central sampling unit and the peripheral sampling units are distributed in a three-dimensional manner to match the full surface of the target tissue.

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