Composition with soothing and brightening effects and application thereof

Abstract

The invention discloses a composition with soothing and brightening effects and application thereof, and the composition comprises the following components: water / glycerol / trehalose / urea / serine / 1, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate. 2-pentanediol / sodium alginate / glycerol polyacrylate / caprylyl glycol / sodium hyaluronate / pullulan / disodium hydrogen phosphate / potassium phosphate, 0.1-1 part of glycerol / water / butanediol / fig fruit extract / EDTA disodium, 0.1-0.8 part of dipropylene glycol / wild hawthorn fruit extract / ginseng root extract / artemisia annua leaf extract / phytosterol, 0.1-0.8 part of 1, 2-pentanediol / sodium alginate / glycerol polyacrylate / caprylyl glycol / sodium hyaluronate / pullulan / disodium hydrogen phosphate / potassium phosphate, 0.1-1 part of glycerol / water / butanediol / fig fruit extract / EDTA disodium, 0.1-0.8 part of 1, 2-pentanediol / sodium alginate / glycerol polyacrylate / caprylyl glycol / sodium hyaluronate / pullulan / disodium hydrogen phosphate / 1, 2-pentanediol / sodium alginate The traditional Chinese medicine composition is prepared from the following components in parts by weight: 0.5 to 2 parts of 1, 3-propylene glycol / glycerol / humulus lupulus flower extract / citric acid, 0.05 to 0.3 part of madecassoside / centella asiatica extract, 0.05 to 0.2 part of cyclodextrin / glabridin and 0.1 to 0.8 part of hydroxyethyl piperazine ethanesulfonic acid. Tests show that the cream prepared from the composition has very good soothing and brightening effects.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, specifically to a composition with soothing and brightening effects and its application. Background Technology

[0002] Sensitive skin has a more fragile skin barrier, making it susceptible to increased sensory nerve signals when stimulated by external factors. This can trigger immune and inflammatory responses in the skin. During inflammation, cell membrane metabolism in keratinocytes, melanocytes, and endothelial cells increases, producing inflammatory mediators such as histamine and leukotrienes. These mediators can affect melanocyte function, leading to post-inflammatory hyperpigmentation. Therefore, developing products with soothing and brightening effects suitable for sensitive skin has a promising market prospect. Summary of the Invention

[0003] In view of the shortcomings of the prior art, the purpose of this invention is to provide a composition with soothing and brightening effects and its application.

[0004] The objective of this invention is achieved through the following technical solution:

[0005] In a first aspect, the present invention provides a composition having soothing and brightening effects, comprising the following components in parts by weight: 0.5-3 parts of water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / short budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate; 0.1-1 parts of glycerin / water / butanediol / fig fruit extract / disodium EDTA; 0.1-0.8 parts of dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols; 0.5-2 parts of 1,3-propanediol / glycerin / hops flower extract / citric acid; 0.05-0.3 parts of asiaticoside / centella asiatica extract; 0.05-0.2 parts of cyclodextrin / glycyrrhizin; and 0.1-0.8 parts of hydroxyethylpiperazine ethanesulfonic acid.

[0006] As a preferred embodiment, the weight ratio of glycerol / water / butanediol / fig fruit extract / disodium EDTA, dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols, 1,3-propanediol / glycerol / hops flower extract / citric acid is 1:1:1.

[0007] As a preferred embodiment, the composition comprises the following components in parts by weight: 1 part of water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / short budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate; 0.5 parts of glycerin / water / butanediol / fig fruit extract / disodium EDTA; 0.5 parts of dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols; 0.5 parts of 1,3-propanediol / glycerin / hops flower extract / citric acid; 0.16 parts of asiaticoside / centella asiatica extract; 0.1 parts of cyclodextrin / glycyrrhizin; and 0.5 parts of hydroxyethylpiperazine ethanesulfonic acid.

[0008] Secondly, the present invention provides the application of the aforementioned composition having soothing and brightening effects in the preparation of skin care products.

[0009] Thirdly, the present invention provides a cream with soothing and brightening effects, comprising the aforementioned composition with soothing and brightening effects, and further comprising at least one of a moisturizer, emulsifier, emulsifier, emulsion stabilizer, thickener, antioxidant, chelating agent, pH adjuster, fragrance, skin conditioner, and solvent.

[0010] As a preferred embodiment, the cream with soothing and brightening effects comprises the following components in parts by weight: water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / short stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate 0.5-3 parts; glycerin / water / butylene glycol / fig fruit extract / disodium EDTA 0.1-1 part; dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols 0.1-0.8 parts; 1,3-propanediol / glycerin / hops flower extract / citric acid 0. 0.5-2 parts, 0.05-0.3 parts of asiaticoside / centella asiatica extract, 0.05-0.2 parts of cyclodextrin / glycyrrhizin, 0.1-0.8 parts of hydroxyethylpiperazine ethane sulfonic acid, 3-15 parts of moisturizer, 2-8 parts of emulsifier, 2-7 parts of emulsifier, 0.5-1.5 parts of co-emulsifier, 0.1-1 part of emulsion stabilizer, 0.2-1.2 parts of thickener, 0.2-1.5 parts of antioxidant, 0.01-0.1 parts of chelating agent, 0.1-0.8 parts of pH adjuster, 0.1-0.2 parts of fragrance, 0.4-2 parts of skin conditioning agent, and solvent to bring the total to 100 parts.

[0011] As a preferred embodiment, the humectant is selected from at least one of 1,3-propanediol, butylene glycol, 1,2-hexanediol, PPG-9 diglyceride, and panthenol.

[0012] As a preferred embodiment, the emollient is selected from at least one of polydimethylsiloxane, behenyl alcohol, caprylic / capric triglyceride, squalane, and coconut oil alcohol-caprylic / capric ester.

[0013] As a preferred embodiment, the emulsifier is selected from at least one of polyglycerol-3-methylglucose distearate, cetearyl oleate / sorbitan oleate, and sodium stearoyl glutamate.

[0014] As a preferred embodiment, the co-emulsifier is selected from cetearyl alcohol.

[0015] As a preferred embodiment, the emulsifying stabilizer is selected from polyacrylamide / water / C13-14 isoparaffin / lauryl alcohol polyether-7.

[0016] As a preferred embodiment, the thickener is selected from at least one of carbomer and xanthan gum.

[0017] As a preferred embodiment, the antioxidant is selected from at least one of p-hydroxyacetophenone and tocopheryl acetate.

[0018] As a preferred embodiment, the chelating agent is selected from disodium EDTA.

[0019] As a preferred embodiment, the pH adjuster is selected from arginine.

[0020] As a preferred option, the fragrance agent is selected from flavorings.

[0021] As a preferred embodiment, the skin conditioning agent is selected from at least one of olive oil PEG-7 esters, allantoin, hydrolyzed sodium hyaluronate, and sodium hyaluronate.

[0022] As a preferred embodiment, the solvent is water.

[0023] Fourthly, the present invention provides a method for preparing a gel-cream with soothing and brightening effects as described above, comprising the following steps:

[0024] The soothing and brightening composition, moisturizer, emulsifier, co-emulsifier, emulsion stabilizer, thickener, antioxidant, chelating agent, pH adjuster, fragrance, and skin conditioning agent are weighed according to the specified weight parts and added to the solvent. After stirring evenly, the soothing and brightening cream is obtained.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] This invention utilizes a composition prepared from water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / short budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate, glycerin / water / butanediol / fig fruit extract / disodium EDTA, dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols, 1,3-propanediol / glycerin / hops flower extract / citric acid, asiaticoside / centella asiatica extract, cyclodextrin / glycyrrhizin, and hydroxyethylpiperazine ethanesulfonic acid, which exhibits significant soothing and brightening effects. Tests showed that the cream prepared using this composition reduced hemoglobin levels by 13.35-17.39% and skin color a* values ​​by 29.21-37.64% after 2 hours of use. The cream prepared using this composition showed an inhibition rate of 21.31-25.38% on tyrosinase activity and 3.34-5.56% on melanin content in cells at a concentration of 1.25% (V / V). Attached Figure Description

[0027] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0028] Figure 1 The self-evaluation results of consumer use tests conducted on the cream prepared in Example 1. Detailed Implementation

[0029] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention. These all fall within the scope of protection of the present invention.

[0030] The terms "preferred," "more preferably," and "more suitable" used in this invention refer to embodiments of the invention that provide certain beneficial effects under certain circumstances. However, other embodiments may also be preferred under the same or other circumstances. Furthermore, the description of one or more preferred embodiments does not imply that other embodiments are unavailable, nor is it intended to exclude other embodiments from the scope of this invention.

[0031] It should be understood that, except in any operational instance or otherwise indicated, all figures representing the amounts of ingredients used, for example, in the specification and claims, should be understood to be modified in all cases by the term "about". Therefore, unless otherwise stated, the numerical parameters set forth in the following specification and appended claims are approximations varying with the desired performance to be obtained according to the invention. It is not at all an attempt to limit the application of the doctrine of equivalents to the scope of the claims; each numerical parameter should be interpreted at least according to the number of significant figures reported and by applying ordinary rounding techniques.

[0032] Although the numerical ranges and parameters illustrating the broad scope of the invention are approximate, the values ​​listed in the specific examples are reported as precisely as possible. However, any numerical value inherently contains some error that is necessarily caused by the standard deviation found in their respective test measurements.

[0033] In the following embodiments of the present invention, a composition having soothing and brightening effects is provided, comprising the following components in parts by weight: water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / short budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate 0.5-3 parts; glycerin / water / butanediol / fig fruit extract / disodium EDTA 0.1-1 parts; dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols 0.1-0.8 parts; 1,3-propanediol / glycerin / hops flower extract / citric acid 0.5-2 parts; hydroxyasiaticoside / centella asiatica extract 0.05-0.3 parts; cyclodextrin / glycyrrhizin 0.05-0.2 parts; hydroxyethylpiperazine ethanesulfonic acid 0.1-0.8 parts.

[0034] In one specific embodiment, the water / glycerol / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glycerol polyacrylate / caprylyl glycol / sodium hyaluronate / budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate is from BASF's A00297 PatcH2O. This commercial raw material contains water, glycerol, trehalose, urea, serine, 1,2-pentanediol, sodium alginate, glycerol polyacrylate, caprylyl glycol, sodium hyaluronate, budding stalk polysaccharide, disodium hydrogen phosphate, and potassium phosphate.

[0035] In one specific embodiment, the glycerol / water / butylene glycol / ficus carica fruit extract / disodium EDTA is a fig protease extract from Hangzhou Shiguang Xinya Biotechnology Co., Ltd. This product contains glycerol, water, butylene glycol, ficus carica fruit extract, and disodium EDTA. Its active ingredient is ficus carica fruit extract.

[0036] In one specific embodiment, the dipropylene glycol / crab (Crataegus cuneata) fruit extract / ginseng (PANAX GINSENG) root extract / Artemisia annua leaf extract / phytosterols are from Shanghai Zhina Biotechnology Co., Ltd.'s Fuyangshu F-192. The raw materials of this product include dipropylene glycol, crataegus cuneata fruit extract, ginseng (PANAX GINSENG) root extract, Artemisia annua leaf extract, and phytosterols. Its active ingredients are crataegus cuneata fruit extract, ginseng (PANAX GINSENG) root extract, and Artemisia annua leaf extract.

[0037] In one specific embodiment, the 1,3-propanediol / glycerin / Humulus lumulus flower extract / citric acid is from Provital SAU 71110-SENSERYN 1TM The ingredients of this product include 1,3-propanediol, glycerin, hops (HUMULUS LUPULUS) flower extract, and citric acid. Its active ingredient is hops (HUMULUS LUPULUS) flower extract.

[0038] In one specific embodiment, the asiaticoside / centella asiatica extract is 90% asiaticoside from Guangxi Changzhou Natural Pharmaceutical Co., Ltd. This commercial raw material contains asiaticoside and centella asiatica extract.

[0039] In one specific embodiment, the cyclodextrin / glabridin is glabridin derived from Jiangsu Tiansheng Pharmaceutical Co., Ltd. This product raw material comprises cyclodextrin and glabridin. Its active ingredient is glabridin.

[0040] In one specific embodiment, the hydroxyethylpiperazine ethane sulfonic acid is ActiBiso-HEPES from Shanghai Bilai Chemical Technology Co., Ltd.

[0041] In the following embodiments, a cream with soothing and brightening effects is also specifically provided, comprising the following components in parts by weight: water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / short stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate 0.5-3 parts; glycerin / water / butanediol / fig fruit extract / disodium EDTA 0.1-1 part; dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols 0.1-0.8 parts; 1,3-propanediol / glycerin / Hops flower extract / citric acid 0.5-2 parts, asiaticoside / centella asiatica extract 0.05-0.3 parts, cyclodextrin / glycyrrhizin 0.05-0.2 parts, moisturizer 3-15 parts, emollient 2-8 parts, emulsifier 2-7 parts, co-emulsifier 0.5-1.5 parts, emulsion stabilizer 0.1-1 part, thickener 0.2-1.2 parts, antioxidant 0.2-1.5 parts, chelating agent 0.01-0.1 parts, pH adjuster 0.1-0.8 parts, fragrance 0.1-0.2 parts, skin conditioning agent 0.4-2 parts, solvent to make up to 100 parts.

[0042] In one specific embodiment, the humectant specifically includes 1,3-propanediol, butylene glycol, 1,2-hexanediol, PPG-9 diglyceride, and panthenol; preferably, the weight ratio of 1,3-propanediol, butylene glycol, 1,2-hexanediol, PPG-9 diglyceride, and panthenol is 1.0-5.0:1.0-5.0:0.5-1:0.5-1:0.1-1.

[0043] In one specific embodiment, the emollient specifically includes polydimethylsiloxane, behenol, caprylic / capric triglyceride, squalane, and coconut oil alcohol-caprylic / capric ester; preferably, the weight ratio of polydimethylsiloxane, behenol, caprylic / capric triglyceride, squalane, and coconut oil alcohol-caprylic / capric ester is 1.0-4.0:0.5-1:0.5-1:0.5-1:0.1-1.

[0044] In one specific embodiment, the emulsifier specifically includes polyglycerol-3-methyl glucoside distearate, cetearyl oleate / sorbitan oleate, and sodium stearoyl glutamate; preferably, the weight ratio of polyglycerol-3-methyl glucoside distearate, cetearyl oleate / sorbitan oleate, and sodium stearoyl glutamate is 1.0-3.0:1.0-3.0:0.1-0.5.

[0045] In one specific embodiment, the co-emulsifier is cetearyl alcohol; the emulsion stabilizer is polyacrylamide / water / C13-14 isoparaffin / lauryl alcohol polyether-7.

[0046] In one specific embodiment, the thickener specifically includes carbomer and xanthan gum. Preferably, the weight ratio of carbomer to xanthan gum is 0.2-1:0.01-0.1.

[0047] In one specific embodiment, the antioxidant specifically comprises p-hydroxyacetophenone and tocopherol acetate. Preferably, the weight ratio of p-hydroxyacetophenone to tocopherol acetate is 0.1-1:0.1-0.5.

[0048] In one specific embodiment, the pH adjuster is arginine; the fragrance is (daily use) perfume; and the solvent is water.

[0049] In one specific embodiment, the skin conditioning agent specifically includes olive oil PEG-7 esters, allantoin, hydrolyzed sodium hyaluronate, and sodium hyaluronate; preferably, the weight ratio of olive oil PEG-7 esters, allantoin, hydrolyzed sodium hyaluronate, and sodium hyaluronate is 0.2-0.8:0.1-0.4:0.1-0.5:0.01-0.1.

[0050] In the following examples, all raw materials used are commercially available.

[0051] Example 1

[0052] This embodiment provides a cream with soothing and brightening effects. The composition and weight ratio of its ingredients are shown in Table 1 (wherein, the weight ratio of water is to bring the total to 100 parts). The preparation steps of the cream are as follows:

[0053] 1) Add water to the mixing bowl, then sprinkle carbomer evenly on the water surface to fully wet it;

[0054] 2) Add hydrolyzed sodium hyaluronate, sodium hyaluronate, and xanthan gum to 1,3-propanediol, disperse evenly, and then add to the main pot;

[0055] 3) Add panthenol, cetearyl oleate / sorbitan oleate, hydroxyethyl piperazine ethane sulfonic acid, sodium stearoyl glutamate, allantoin, and disodium EDTA to the mixing bowl, turn on the stirring, turn on the heating, and heat to 80-85℃.

[0056] 4) Add polydimethylsiloxane, polyglycerol-3-methylglucose distearate, behenol, caprylic / capric triglyceride, squalane, coconut oil alcohol-caprylate / capric acid ester, cetearyl alcohol, and tocopheryl acetate to the oil pan, start stirring, start heating, heat to 80-85℃, then transfer to the main pot, start homogenization in the main pot, homogenize at 3000 rpm for 3-5 minutes, then add polyacrylamide / water / C13-14 isoparaffin / lauryl alcohol polyether-7, and continue homogenization for 3-4 minutes;

[0057] 5) Dissolve the arginine in water until it is evenly dissolved, then add it to the main pot. You can gently homogenize or stir it evenly, and then start cooling.

[0058] 6) After the main pot cools down to 40-45℃, dissolve the hydroxyascorbic acid / centella asiatica extract and cyclodextrin / glycyrrhizin in water and add them to the pot.

[0059] 7) Continue to add PPG-9 diglyceride, water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate, glycerin / water / butanediol / ficus carica fruit extract / disodium EDTA, dipropylene glycol / hawthorn fruit extract / ginseng root extract / artemisia ananaana leaf extract / phytosterols, 1,3-propanediol / glycerin / humulus lupulus flower extract / citric acid, olive oil PEG-7 esters, and (daily use) fragrance to the mixing bowl, and stir well;

[0060] 8) After heating and dissolving butanediol, 1,2-hexanediol and p-hydroxyacetophenone evenly, add them to the main pot, stir evenly, cool to 38°C, and inspect the discharge to obtain a cream with soothing and brightening effects.

[0061] Example 2

[0062] This embodiment provides a cream with soothing and brightening effects. The composition and weight ratio of its ingredients are shown in Table 1 (wherein, the weight ratio of water is to make up to 100 parts). The preparation steps of the cream are the same as in Example 1.

[0063] Example 3

[0064] This embodiment provides a cream with soothing and brightening effects. The composition and weight ratio of its ingredients are shown in Table 1 (wherein, the weight ratio of water is to make up to 100 parts). The preparation steps of the cream are the same as in Example 1.

[0065] Example 4

[0066] This embodiment provides a cream with soothing and brightening effects. The composition and weight ratio of its ingredients are shown in Table 1 (wherein, the weight ratio of water is to make up to 100 parts). The preparation steps of the cream are the same as in Example 1.

[0067] Example 5

[0068] This embodiment provides a cream with soothing and brightening effects. The composition and weight ratio of its ingredients are shown in Table 1 (wherein, the weight ratio of water is to make up to 100 parts). The preparation steps of the cream are the same as in Example 1.

[0069] Example 6

[0070] This embodiment provides a cream with soothing and brightening effects. The composition and weight ratio of its ingredients are shown in Table 1 (wherein, the weight ratio of water is to make up to 100 parts). The preparation steps of the cream are the same as in Example 1.

[0071] Table 1

[0072]

[0073]

[0074] Comparative Example 1

[0075] This comparative example provides a cream with soothing and brightening effects, the composition and weight ratio of its ingredients are shown in Table 2 (wherein the weight ratio of water is to be made up to 100 parts). The only difference from Example 2 is that glycerin / water / butylene glycol / fig extract / disodium EDTA are not added, and the weight ratio of water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate is 3 parts. The preparation steps of the cream are the same as in Example 1.

[0076] Comparative Example 2

[0077] This comparative example provides a cream with soothing and brightening effects. The composition and weight proportions of its ingredients are shown in Table 2 (wherein the weight proportion of water is to be made up to 100 parts). The only difference from Example 2 is that it does not contain dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols, and the weight proportions of water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / short stalk budding enzyme polysaccharide / disodium hydrogen phosphate / potassium phosphate are 2.3 parts. The preparation steps of the cream are the same as in Example 1.

[0078] Comparative Example 3

[0079] This comparative example provides a cream with soothing and brightening effects. The composition and weight ratio of its ingredients are shown in Table 2 (wherein the weight of water is to be made up to 100 parts). The only difference from Example 2 is that 1,3-propanediol / glycerin / hop flower extract / citric acid are not added, and the weight ratio of water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate is 2.5 parts. The preparation steps of the cream are the same as in Example 1.

[0080] Comparative Example 4

[0081] This comparative example provides a cream with soothing and brightening effects, the composition and weight ratio of its ingredients are shown in Table 2 (wherein the weight ratio of water is to be made up to 100 parts). The only difference from Example 2 is that: hydroxyascorbic acid glycoside / centella asiatica extract is not added, and the weight ratio of water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate is 2.2 parts. The preparation steps of the cream are the same as in Example 1.

[0082] Comparative Example 5

[0083] This comparative example provides a cream with soothing and brightening effects. The composition and weight proportions of its ingredients are shown in Table 2 (wherein the weight proportion of water is to be made up to 100 parts). The only difference from Example 2 is that cyclodextrin / glycyrrhizin are not added, and the weight proportions of water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate are 2.05 parts. The preparation steps of the cream are the same as in Example 1.

[0084] Comparative Example 6

[0085] This comparative example provides a cream with soothing and brightening effects, the composition and weight ratio of its ingredients are shown in Table 2 (wherein the weight ratio of water is to be made up to 100 parts). The only difference from Example 2 is that hydroxyethylpiperazine ethanesulfonic acid is not added, and the weight ratio of water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate is 2.8 parts. The preparation steps of the cream are the same as in Example 1.

[0086] Table 2

[0087]

[0088]

[0089] Efficacy verification:

[0090] The creams prepared in Examples 1-6 and Comparative Examples 1-6 were used as test products to test their soothing and brightening effects, and the cream prepared in Example 1 was used in consumer testing.

[0091] 1) Soothing efficacy test

[0092] Human efficacy evaluation testing was conducted, based on T / GDCDC 021-2022 "Test Methods for Soothing Efficacy of Cosmetics". The test indicators were skin hemoglobin content and skin color a* value. The specific testing steps are as follows:

[0093] 1.1 Select healthy men and women aged 18-60 years with healthy skin and no obvious scars or other defects that may affect the test results as subjects.

[0094] 1.2 Before the test, the subjects should rinse the test sites with clean water and dry the inner sides of their forearms with a dry paper towel. Two measurement areas should be marked on the inner sides of the left and right forearms, each measuring 3cm x 3cm, with at least 1cm between each area. Before the formal test, the subjects should sit quietly in a compliant room for at least 30 minutes without drinking water or beverages. The forearms should be exposed, placed in the test position, and kept relaxed.

[0095] 1.3 Using Mexameter and SkinColorCatch, the initial values ​​(Tinitial) of skin hemoglobin content and skin color a* value of each test area were first measured. Then, hypoallergenic tape was used to peel off each test area 15 times. After peeling, the skin hemoglobin content and skin color a* value of each test area were measured again as the pre-use measurement values, and the data were recorded as T0.

[0096] 1.4 Each test area was divided into a product area and a control area. In the product area, the concentration was (2.0±0.1) mg / cm³. 2 Apply the appropriate amount of product to each test area, while leaving the control area untreated. After 2 hours, measure the hemoglobin and skin color a* values ​​in both the product and control areas as post-use measurements, and record the data as T2.

[0097] 1.5 The following formulas were used to calculate the rate of change of hemoglobin values ​​and the rate of change of skin color a* values ​​for each test sample group and the blank control group:

[0098] Rate of change = (Measurement after use - Measurement before use) / Measurement before use * 100%

[0099] The test results of the rate of change of heme value and the rate of change of skin color a* value for each test sample group and the blank control group are shown in Table 3 (the data in Table 3 are the average values ​​of multiple test data, "-" indicates a decrease).

[0100] Table 3

[0101]

[0102]

[0103] As shown in Table 3, the creams prepared in Examples 1-6 have excellent soothing effects. Compared with before use (T0), the rate of change in hemoglobin value decreased by 13.35-17.39% and the rate of change in skin color a* value decreased by 29.21-37.64% after 2 hours of use. In particular, Example 1 showed the best soothing effect, with a 17.39% decrease in the rate of change in hemoglobin value and a 37.64% decrease after 2 hours of use. Furthermore, a comparison of the results of Example 1 with Examples 5-6 shows that when the weight ratio of glycerin / water / butanediol / fig extract / disodium EDTA, dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols, 1,3-propanediol / glycerin / hops flower extract / citric acid in the soothing and brightening composition used in the cream is 1:1:1, the rate of change in hemoglobin value and skin color a* value can be further reduced after use.

[0104] As can be seen from the comparison between Example 2 and Comparative Examples 1-6, when any one of the following is missing from the composition with soothing and brightening effects used in the cream: glycerin / water / butanediol / fig fruit extract / disodium EDTA, dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols, 1,3-propanediol / glycerin / hops flower extract / citric acid, asiaticoside / centella asiatica extract, cyclodextrin / glycyrrhizin, or hydroxyethylpiperazine ethanesulfonic acid, the creams prepared in Comparative Examples 1-6 all resulted in varying degrees of worse heme value change rate and skin color a* value change rate compared to the cream in Example 2. This indicates that the components in the composition with soothing and brightening effects can work together synergistically to improve the soothing effect.

[0105] 2) Brightening effect test

[0106] Based on melanocytes, the effects of test samples prepared in each embodiment and comparative example on melanocyte tyrosinase activity and melanin synthesis were examined.

[0107] The specific steps are as follows:

[0108] 2.1 The creams prepared in each example and comparative example were added to PBS buffer (purchased from Yuanpei Biotechnology) to prepare test samples. A blank matrix was prepared by adding water, glycerol, trehalose, urea, serine, 1,2-pentanediol, sodium alginate, glyceryl polyacrylate, caprylyl glycol, sodium hyaluronate, budding stalk polysaccharide, disodium hydrogen phosphate, potassium phosphate, glycerol, water, butylene glycol, fig fruit extract, disodium EDTA, dipropylene glycol, hawthorn fruit extract, ginseng root extract, Artemisia annua leaf extract, phytosterols, 1,3-propanediol, glycerol, hop flower extract, citric acid, asiaticoside, centella asiatica extract, cyclodextrin, glycyrrhizin, and hydroxyethylpiperazine ethanesulfonic acid, with water added to make up the balance. The blank matrix was then added to PBS buffer to prepare blank matrix samples.

[0109] 2.2 Logarithmic growth phase mouse melanoma cells B16F10 (purchased from Beina Biotechnology) were collected at a cell density of 2×10⁻⁶ cells / year. 4 Cells / well were seeded into 96-well plates containing basal culture medium (purchased from Yuanpei Biotechnology). After culturing in an incubator (37℃, 5% CO2) for 24 h, each test sample and blank matrix sample were added to the plate at a final concentration of 1.25% (V / V) as the drug treatment group, and untreated cells were used as the negative control group. Each group was set up in 3 replicates. Cells in each group were cultured for 48 hours in an incubator (37℃, 5% CO2). The culture medium was discarded, and the cells were washed twice with PBS. 100 μL of 0.5% Triton X-100 was added to each well, and the cells were lysed at -80℃ for 2 hours. The 96-well plates were then removed, allowed to thaw naturally at room temperature, and 50 μL of 1 mg / mL levodopa (purchased from Adamas-beta) was added to each well. The plates were incubated at 37℃ for 2 hours, with 50 μL of 1 mg / mL levodopa used as a control group. The absorbance of each group was read at 475 nm (using a Supermax 3100 microplate reader), and the inhibition rate of tyrosinase activity in each treatment group was calculated using the following formula:

[0110] Tyrosinase activity inhibition rate (%) = (1-(A) 给药组 -A 空白组 ) / (A 阴性对照组 -A 空白组 ))×100%

[0111] Among them, A 给药组 A 空白组 A 阴性对照组 The absorbance values ​​were measured for each treatment group, negative control group, and blank group, respectively.

[0112] 2.3 Logarithmic growth phase mouse melanoma cells B16F10 (purchased from Beina Biotechnology) were collected at a cell density of 2×10⁻⁶ cells / year. 5Cells were seeded per well in 12-well plates, and the basal medium was replaced with high-glucose DMEM medium (purchased from Yuanpei Biotechnology) to induce melanin production in the cells. After culturing for 24 h in an incubator (37℃, 5% CO2), each test sample and blank matrix sample were added to the treatment group at a final concentration of 1.25% (V / V). Untreated cells served as the negative control group, and each group was divided into three replicates. Cells in each group were cultured for another 48 h in an incubator (37℃, 5% CO2). The supernatant was discarded, and 0.5 mL of 1M NaOH containing 10% DMSO was added. The cells were incubated at 80–90℃ for 3 h, and after returning to room temperature, 200 μL of each cell was transferred to a 96-well plate. 1M NaOH containing 10% DMSO served as the blank group. The absorbance of each group was read at 405 nm (using a Supermax 3100 microplate reader), and the relative melanin inhibition rate of each treatment group was calculated using the following formula:

[0113] Relative melanin inhibition rate (%) = (1-(A) 给药组 -A 空白组 ) / (A 阴性对照组 -A 空白组 ))×100% where, A 给药组 A 空白组 A 阴性对照组 The absorbance values ​​were measured for each treatment group, negative control group, and blank group, respectively.

[0114] The results of the inhibition rates of cellular tyrosinase activity and the relative inhibition rates of cellular melanin obtained from the test samples used in each drug administration group are shown in Table 4.

[0115] Table 4

[0116]

[0117]

[0118] As shown in Table 4, the creams prepared in Examples 1-6 have excellent brightening effects, inhibiting tyrosinase activity in cells by 21.31-25.38% and melanin content by 3.34-5.56% at a concentration of 1.25% (V / V). Example 1, in particular, exhibited the best brightening effect, achieving an inhibition rate of 25.38% on tyrosinase activity and 5.56% on melanin content.

[0119] As can be seen from the comparison between Example 2 and Comparative Examples 1-6, when any one of the following is missing from the composition used in the cream for soothing and brightening effects: glycerin / water / butanediol / fig fruit extract / disodium EDTA, dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols, 1,3-propanediol / glycerin / hops flower extract / citric acid, asiaticoside / centella asiatica extract, cyclodextrin / glycyrrhizin, or hydroxyethylpiperazine ethanesulfonic acid, the prepared cream will show varying degrees of decrease in its inhibition rate of tyrosinase activity and melanin content. This indicates that the components in the composition used for soothing and brightening effects can work together synergistically to improve the brightening effect.

[0120] 3) Consumer usage testing

[0121] This test evaluates the product's user experience and efficacy through 14 consecutive days of product use and self-assessment by the participants.

[0122] The selection criteria for the subjects were: women with sensitive skin who were prone to sensitivity in their private parts, who considered themselves to be sensitive in their private parts, and aged between 18 and 60 years old. A total of 30 subjects were included.

[0123] The method of use is as follows: On dry skin, apply an appropriate amount of the cream prepared in Example 1 evenly to clean, intimate skin, and gently massage until absorbed. Apply more to dry areas. After 14 days of continuous use, complete a self-assessment questionnaire.

[0124] Consumer self-evaluation results after 14 days of product use are as follows: Figure 1 As shown.

[0125] This invention has many specific applications, and the above description is only a preferred embodiment. It should be noted that the above embodiments are for illustrative purposes only and are not intended to limit the scope of protection of this invention. For those skilled in the art, several improvements can be made without departing from the principle of this invention, and these improvements should also be considered within the scope of protection of this invention.

Claims

1. A composition having soothing and brightening effects, characterized in that, The product comprises the following components in parts by weight: water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / short stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate 0.5-3 parts; glycerin / water / butanediol / fig fruit extract / disodium EDTA 0.1-1 part; dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols 0.1-0.8 parts; 1,3-propanediol / glycerin / hops flower extract / citric acid 0.5-2 parts; asiaticoside / centella asiatica extract 0.05-0.3 parts; cyclodextrin / glycyrrhizin 0.05-0.2 parts; and hydroxyethylpiperazine ethanesulfonic acid 0.1-0.8 parts.

2. The composition with soothing and brightening effects according to claim 1, characterized in that, The weight ratio of glycerol / water / butanediol / fig fruit extract / disodium EDTA, dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols, 1,3-propanediol / glycerol / hops flower extract / citric acid is 1:1:

1.

3. The composition with soothing and brightening effects according to claim 1 or 2, characterized in that, The composition comprises the following components in parts by weight: 1 part of water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / short budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate; 0.5 parts of glycerin / water / butanediol / fig fruit extract / disodium EDTA; 0.5 parts of dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols; 0.5 parts of 1,3-propanediol / glycerin / hops flower extract / citric acid; 0.16 parts of asiaticoside / centella asiatica extract; 0.1 parts of cyclodextrin / glycyrrhizin; and 0.5 parts of hydroxyethylpiperazine ethanesulfonic acid.

4. The use of a composition having soothing and brightening effects according to any one of claims 1-2 in the preparation of skin care products.

5. A gel-cream with soothing and brightening effects, characterized in that, The composition comprising the soothing and brightening effects as described in any one of claims 1-2 further comprises at least one of the following: a moisturizer, a skin emulsifier, an emulsifier, a co-emulsifier, an emulsion stabilizer, a thickener, an antioxidant, a chelating agent, a pH adjuster, a fragrance, a skin conditioning agent, and a solvent.

6. The cream with soothing and brightening effects according to claim 5, characterized in that, The product comprises the following components in parts by weight: water / glycerin / trehalose / urea / serine / 1,2-pentanediol / sodium alginate / glyceryl polyacrylate / caprylyl glycol / sodium hyaluronate / budding stalk polysaccharide / disodium hydrogen phosphate / potassium phosphate 0.5-3 parts; glycerin / water / butanediol / fig fruit extract / disodium EDTA 0.1-1 part; dipropylene glycol / hawthorn fruit extract / ginseng root extract / Artemisia annua leaf extract / phytosterols 0.1-0.8 parts; 1,3-propanediol / glycerin / hops flower extract / citric acid 0.5-2 parts; and hydroxyadenophora stricta. Glycoside / Centella asiatica extract 0.05-0.3 parts, cyclodextrin / glycyrrhizin 0.05-0.2 parts, hydroxyethylpiperazine ethane sulfonic acid 0.1-0.8 parts, moisturizer 3-15 parts, skin moisturizer 2-8 parts, emulsifier 2-7 parts, co-emulsifier 0.5-1.5 parts, emulsion stabilizer 0.1-1 part, thickener 0.2-1.2 parts, antioxidant 0.2-1.5 parts, chelating agent 0.01-0.1 parts, pH adjuster 0.1-0.8 parts, fragrance 0.1-0.2 parts, skin conditioning agent 0.4-2 parts, solvent to make up to 100 parts.

7. The cream with soothing and brightening effects according to claim 5 or 6, characterized in that, The humectant is selected from at least one of 1,3-propanediol, butylene glycol, 1,2-hexanediol, PPG-9 diglyceride, and panthenol; The emollient is selected from at least one of polydimethylsiloxane, behenol, caprylic / capric triglyceride, squalane, and coconut oil alcohol-caprylic / capric ester; The emulsifier is selected from at least one of polyglycerol-3-methyl glucoside distearate, cetearyl oleate / sorbitan oleate, and sodium stearoyl glutamate. The co-emulsifier is selected from cetearyl alcohol; The emulsifying stabilizer is selected from polyacrylamide / water / C13-14 isoparaffin / laurate polyether-7.

8. The cream with soothing and brightening effects according to claim 5 or 6, characterized in that, The thickener is selected from at least one of carbomer and xanthan gum; The antioxidant is selected from at least one of p-hydroxyacetophenone and tocopheryl acetate; The chelating agent is selected from disodium EDTA; The pH adjuster is selected from arginine; The fragrance agent is selected from flavorings.

9. The cream with soothing and brightening effects according to claim 5 or 6, characterized in that, The skin conditioning agent is selected from at least one of olive oil PEG-7 esters, allantoin, hydrolyzed sodium hyaluronate, and sodium hyaluronate. The solvent is water.

10. A method for preparing a gel-cream with soothing and brightening effects according to any one of claims 5-9, characterized in that, Includes the following steps: The soothing and brightening composition, moisturizer, emulsifier, co-emulsifier, emulsion stabilizer, thickener, antioxidant, chelating agent, pH adjuster, fragrance, and skin conditioning agent are weighed according to the specified weight parts and added to the solvent. After stirring evenly, the soothing and brightening cream is obtained.

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