Synergistic whitening composition, application thereof and cosmetics
By using a specific ratio of quercetin and tetrahydrocurcumin, a synergistic whitening composition is formed, which solves the problems of high cost and poor effect of existing whitening products, and achieves efficient whitening and cost-saving effects.
Patent Information
- Application Number
- CN202511219214.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-28
- Publication Date
- 2025-11-14
AI Technical Summary
Among existing whitening products, the classic plant extract glycyrrhizin is expensive, resulting in low dosage and difficulty in achieving significant whitening effects. In addition, traditional active ingredients are highly irritating, and there is a lack of natural and effective whitening compositions on the market.
A synergistic whitening composition is formed by using a specific mass ratio of quercetin and tetrahydrocurcumin for use in cosmetics, reducing the dosage and improving the whitening effect.
The synergistic combination of quercetin and tetrahydrocurcumin significantly enhances the whitening effect, inhibits melanin production, achieves better whitening results, and reduces costs.
Smart Images
Figure CN120938837A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of cosmetic technology, and in particular to a synergistic whitening composition, its uses, and cosmetics. Background Technology
[0002] Chinese consumers have a persistent demand for skin whitening products, resulting in a wide variety of products and technologies on the market. However, many of these whitening products contain resorcinol-based active ingredients, which are often quite irritating. As people's living standards improve, their demands for quality of life also increase, leading to a growing preference for cosmetics using various natural plant extracts as whitening active ingredients. However, the classic plant extract glycyrrhizin is expensive, so it is added in very small quantities to products, making it difficult to achieve a truly whitening effect.
[0003] Quercetin, also known as quinacrine, is a natural flavonoid with strong biological activity. It is widely found in various fruits and vegetables and has antioxidant, anti-inflammatory, and whitening effects.
[0004] Tetrahydrocurcumin is a natural functional whitening ingredient extracted from the rhizome of turmeric (Curcuma longa), a plant in the ginger family. It inhibits the activity of tyrosinase and the generation of oxygen free radicals, exhibiting significant antioxidant effects. It is widely used in whitening, spot-removing, and antioxidant skincare products. Summary of the Invention
[0005] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a whitening composition, its use and cosmetics, which utilizes the reasonable ratio of ellagic acid and tetrahydromagnoliol to produce a synergistic whitening effect, and can reduce the amount of both used in the product, thereby saving costs while achieving better whitening effect.
[0006] In a first aspect, this application provides a skin whitening composition comprising quercetin and tetrahydrocurcumin, wherein the mass ratio of quercetin to tetrahydrocurcumin is 16:1 to 1:8.
[0007] In some embodiments, the mass ratio of quercetin to tetrahydrocurcumin is 8:1 to 1:8.
[0008] In some embodiments, the mass ratio of quercetin to tetrahydrocurcumin is 4:1 to 1:4.
[0009] In some embodiments, the mass ratio of quercetin to tetrahydrocurcumin is 2:1 to 1:2.
[0010] Secondly, the present invention provides the use of the synergistic whitening composition as described in any of the above embodiments in the preparation of cosmetics.
[0011] In some embodiments, the synergistic whitening composition can synergistically inhibit melanin production.
[0012] In some embodiments, the cosmetic includes at least one of serum, gel, lotion, foundation, or cream.
[0013] Thirdly, this application provides a cosmetic product comprising the whitening composition described in any one of the above embodiments.
[0014] In some embodiments, the quercetin concentration in the cosmetic product is 0.01 wt% to 0.5 wt%.
[0015] In some embodiments, the tetrahydrocurcumin in the cosmetic has a mass percentage concentration of 0.01 wt% to 0.5 wt%.
[0016] Compared with the prior art, the present invention has the following beneficial effects:
[0017] Compared to existing technologies, this invention provides a composition with synergistic whitening effects, comprising quercetin and tetrahydrocurcumin in specific mass ratios. In the combination provided by this invention, quercetin and tetrahydrocurcumin exhibit synergistic whitening effects; in inhibiting melanin production, the composition's efficacy is significantly greater than the sum of the effects of using quercetin alone or using tetrahydrocurcumin alone. Attached Figure Description
[0018] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:
[0019] Figure 1 The graphs show the melanin content test results of the blank control group, positive control group, comparative examples 2, 7 and 5 in this application.
[0020] Figure 1 In the figures: a is the blank control group, b is the positive control group, c is comparative example 2, d is comparative example 7, and e is example 5. The scale bar is 50 μm. Detailed Implementation
[0021] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention. These all fall within the scope of protection of the present invention.
[0022] Examples 1-8, Comparative Examples 1-11
[0023] The raw material formulations of the whitening compositions of Examples 1-8 and Comparative Examples 1-11 in this invention are shown in Table 1. Among them, Comparative Examples 1-10 are single-factor control experiments.
[0024] The melanin-inhibiting effects of the products obtained in Examples 1-8 and Comparative Examples 1-11 were tested, and the specific methods were as follows:
[0025] Testing system: The cells used were melanocytes, batch number: MC25021302, provided by Guangdong Boxi Shaanxi Branch.
[0026] Main reagents: M-254 culture medium (Guangdong Boxi Shaanxi Branch), PBS (Solepro), L-DOPA (Sigma), BCA kit (Beyotime), MTT (Sigma), DMSO (Sigma), silver staining kit (Solepro), paraformaldehyde (Biosharp).
[0027] Main equipment: CO2 incubator (Thermo, 1501), laminar flow hood (Sujing Antai, SW-CJ-2F), microplate reader (BioTek, Epoch), upright microscope (Olympus, BX53).
[0028] Cytotoxicity test methods:
[0029] 1. Cell seeding: After the cells have been revived, when the plating rate reaches about 60%, seed the cells into 96-well plates and incubate overnight in a CO2 incubator (37℃, 5v / v% CO2).
[0030] 2. Experimental Groups: The experiment included a blank control group, a positive control group, comparative examples (Comparative Example 2 and Comparative Example 7), and an example group. In the examples, each sample was configured with 8 concentration gradients (corresponding to Examples 1-8), and 3 replicate wells were set for each concentration gradient.
[0031] 3. Solution preparation: Prepare working solutions of different concentrations according to the test concentration setting table.
[0032] 4. The cytotoxicity test results of quercetin and tetrahydrocurcumin are shown in Tables 2 and 3, respectively.
[0033] Methods for testing melanin content:
[0034] 1. Cell seeding: After cell resuscitation, when the cell plating rate reaches about 60%, seed the cells into 6-well plates and incubate overnight in a CO2 incubator (37℃, 5v / v% CO2).
[0035] 2. Solution preparation: Prepare the working solution of the test substance according to the test group. The positive control is 42.67 ppm kojic acid.
[0036] 3. Drug administration: According to the test groups, when the cell deposition rate in the 6-well plate reaches 40%–60%, drug administration is performed in groups, with 3 replicates per group. 2 mL of culture medium is added to each well of the blank control group, 2 mL of culture medium containing kojic acid is added to each well of the positive control group, and 2 mL of culture medium containing the corresponding concentration of the test sample is added to each well of the sample group. After drug administration, the 6-well plate is placed in a CO2 incubator (37℃, 5v / v% CO2) and incubated for 72 h. After 72 h, the medium is discarded, and the plate is washed once with PBS.
[0037] 4. Melanin content test: After cell culture, fix with 4wt% paraformaldehyde solution, stain according to the silver staining kit instructions, and photograph under a microscope. Figure 1 As shown, red represents stained melanocytes, and black represents secreted melanin. The amount of melanin produced in Comparative Examples 2 and 7 is higher than that in Example 5.
[0038] 5. Inhibition rate calculation: Inhibition rate (%) = (blank control group - sample group) / blank control group × 100%.
[0039] Synergistic effect assessment method: Bliss independence method.
[0040] In the Bliss model, the theoretical joint inhibition rate is: E A+B =E A +E B -E A *E B
[0041] If the actual combined inhibition rate is greater than the theoretical combined inhibition rate, then components A and B are determined to have a synergistic effect.
[0042] Results of melanin inhibition tests in Comparative Examples 1-11 and Examples 1-8
[0043]
[0044] Table 2. Quercetin cytotoxicity test results
[0045]
[0046] Table 3 Results of tetrahydrocurcumin cytotoxicity test
[0047]
[0048] The E theory of Example 1 was calculated from the data of Comparative Example 1 and Comparative Example 7; the E theory of Example 2 was calculated from the data of Comparative Example 1 and Comparative Example 8; the E theory of Example 3 was calculated from the data of Comparative Example 1 and Comparative Example 9; the E theory of Example 4 was calculated from the data of Comparative Example 1 and Comparative Example 10; the E theory of Example 5 was calculated from the data of Comparative Example 2 and Comparative Example 7; the E theory of Example 6 was calculated from the data of Comparative Example 3 and Comparative Example 7; the E theory of Example 7 was calculated from the data of Comparative Example 4 and Comparative Example 7; and the E theory of Example 8 was calculated from the data of Comparative Example 5 and Comparative Example 7.
[0049] Comparative Examples 1–10 were single-component administrations, and E-theory was not calculated.
[0050] The E-theory of Comparative Example 11 was calculated from the data of Comparative Example 1 and Comparative Example 10, and the E-theory of Comparative Example 12 was calculated from the data of Comparative Example 6 and Comparative Example 7.
[0051] Comparing the theoretical E and actual E of Examples 1-8 and Comparative Example 11, a synergistic determination is made:
[0052] If actual efficiency (E) > theoretical efficiency (E), then efficiency will be increased.
[0053] If Eactual < Etheoretical, then it is antagonistic;
[0054] If Eactual = Etheoretical, it is an addition.
[0055] The test results and synergistic determination of Examples 1-8 and Comparative Examples 1-11 are shown in Table 1. In Example 1, the theoretical E of 0.5 ppm quercetin and 0.25 ppm tetrahydrocurcumin is 70.5%, and the actual E of Example 1 is 87%. Since the actual E is greater than the theoretical E, it proves that quercetin and tetrahydrocurcumin have a synergistic effect in melanin inhibition when they are in a 2:1 ratio.
[0056] Similarly, the actual values of E in Examples 2 to 8 are all greater than the theoretical values of E, indicating that when the mass ratio of quercetin to tetrahydrocurcumin is in the range of 16:1 to 1:8, quercetin and tetrahydrocurcumin have a synergistic effect in inhibiting melanin.
[0057] In contrast, the actual E in Comparative Example 11 is less than the theoretical E, indicating that quercetin and tetrahydrocurcumin do not have a synergistic effect in inhibiting melanin under the ratio of Comparative Example 11.
[0058] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various modifications or variations within the scope of the claims, which do not affect the essence of the present invention.
Claims
1. A synergistic whitening composition, characterized in that, It includes quercetin and tetrahydrocurcumin, wherein the mass ratio of quercetin to tetrahydrocurcumin is 16:1 to 1:
8.
2. The synergistic whitening composition according to claim 1, characterized in that, The mass ratio of quercetin to tetrahydrocurcumin is 8:1 to 1:
8.
3. The synergistic whitening composition according to claim 2, characterized in that, The mass ratio of quercetin to tetrahydrocurcumin is 4:1 to 1:
4.
4. The synergistic whitening composition according to claim 3, characterized in that, The mass ratio of quercetin to tetrahydrocurcumin is 2:1 to 1:
2.
5. Use of the synergistic whitening composition according to any one of claims 1 to 4 in the preparation of cosmetics.
6. The use according to claim 5, characterized in that, The synergistic whitening composition can synergistically inhibit melanin production.
7. The use according to claim 5, characterized in that, The cosmetics include at least one of serum, gel, lotion, foundation, or cream.
8. A cosmetic product, characterized in that, Includes the whitening composition according to any one of claims 1 to 4.
9. The cosmetic product according to claim 8, characterized in that, The quercetin concentration in the cosmetic product is 0.01 wt% to 0.5 wt%.
10. The cosmetic product according to claim 8, characterized in that, The tetrahydrocurcumin has a mass percentage concentration of 0.01 wt% to 0.5 wt% in the cosmetic.