Processing method of radix aconiti carmichaeli (Limjiang slices)

Abstract

The invention relates to the technical field of traditional Chinese medicine decoction piece processing, and particularly discloses a processing method of radix aconiti carmichaeli (Linjiang tablets). The invention relates to a traditional processing technology of 'Cinnamomum camphora' characteristic, which solves the problems of unstable toxic components and large quality fluctuation of processed products in the prior art by accurately controlling the soaking and bleaching time sequence, the steaming time length and the drying temperature. Through processing, the mass fraction of the toxic component mesaconitine in the radix aconiti carmichaeli is reduced to be not detected from 0.0300% before processing; the mass fraction of hypaconitine and the mass fraction of aconitine are both reduced from 0.0156% to 0.0064% to below ten thousandth. The mass fraction of the effective component benzoyl mesaconitine is increased to 0.0097% and 0.0013% from 0.0034% and 0.0004% before processing, and the mass fractions of the effective component benzoyl mesaconitine and the benzoyl mesaconitine are respectively increased to 285.97% and 353.59%. According to the processing method, the content of toxic components in the salted radix aconiti lateralis praeparata can be remarkably reduced, the content of effective components can be increased, the effects of reducing toxicity and increasing efficiency are achieved, the safety index of the Chinese pharmacopoeia is met, and the processing method is suitable for standardized production of toxic decoction pieces of radix aconiti lateralis praeparata.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine processing technology, and in particular to a processing method for Aconitum carmichaelii (Linjiang slices). Background Technology

[0002] Aconite (Fuzi) is the root of the plant *Aconitum carmichaelii* Debx., belonging to the Ranunculaceae family. It is a commonly used warming herb in Traditional Chinese Medicine (TCM) with the functions of restoring yang, tonifying fire and assisting yang, and dispelling cold and relieving pain. Aconite is pungent, sweet, and very hot in nature. It readily travels through the twelve meridians and the Governing Vessel, guiding fire back to its origin, warming the interior of the Lesser Yin meridian, and tonifying the true yang of the Gate of Life. It is an extremely effective and important herb for treating yang deficiency and collapse, and its medicinal history can be traced back to the *Shennong Bencao Jing* (Shennong's Classic of Materia Medica). As the "leader of all herbs," aconite holds an irreplaceable and important position in clinical applications, especially in restoring yang and rescuing from collapse, where it is hailed as the "number one herb for restoring yang and rescuing from collapse."

[0003] However, Aconitum carmichaelii contains aconitine alkaloids (such as aconitine, hypoaconitine, and neoaconitine), which are highly toxic. Therefore, it requires strict processing before clinical use to reduce toxicity and preserve or enhance its efficacy. The processing of Aconitum carmichaelii has a long history, with records of its use dating back to the Han Dynasty in the *Treatise on Febrile Diseases*. Physicians throughout history have developed various processing methods based on this, such as steaming, boiling, and processing with bile, forming a rich system of processing techniques. Modern pharmacological research has confirmed that diester alkaloids in Aconitum carmichaelii are its main toxic components, while monoester alkaloids have cardiotonic and hypoxia-resistant effects. Preserving or enhancing efficacy while reducing toxicity is the core issue in Aconitum carmichaelii processing research.

[0004] The Zhangbang school, representing one of the traditional Chinese medicine processing techniques in Jiangxi, is renowned for its distinctive processed medicinal slices such as "Linjiang Slices," "White Peony Root Soaring to the Sky," and "A Single Thread of Tangerine Peel." Its processing techniques are centered on "exquisite knife work, meticulous use of excipients, and unique control of heat," particularly adept at reducing toxicity and enhancing efficacy through the synergistic control of excipients and heat. The Zhangbang-processed Aconitum carmichaelii (Linjiang Slices) is characterized by its use of "ginger and rice water processing combined with prolonged steaming" to transform the raw Aconitum carmichaelii into a cooked product, achieving the goal of "reducing toxicity and increasing efficacy." This involves multiple processes including soaking, rinsing, steaming, and slicing to moderate the medicinal properties and decompose toxic components. The Zhangbang Aconitum carmichaelii processing technique has been passed down for over a thousand years and is one of the traditional local advantages of the "Zhangbang" school of Chinese medicine in Jiangxi. However, current research and reports on Linjiang Slices are scarce, with insufficient research on the processing mechanism, a lack of local quality standards, and unclear mechanisms of action; furthermore, Linjiang Slices are not included in the *Chinese Pharmacopoeia*, severely limiting their clinical application. In addition, compared with other traditional Chinese medicine products such as light-processed aconite root and salt-processed aconite root, Linjiang tablets have unique advantages in restoring yang and rescuing from collapse, and in tonifying yang and dispelling cold.

[0005] This invention not only provides a novel method for the processing of toxic Chinese medicinal herbs, but also provides scientific support for the differences in chemical components that contribute to the "detoxification and efficacy enhancement" effect of Aconitum carmichaelii after processing with camphor trees. Summary of the Invention

[0006] Based on the above background, the technical problem to be solved by the present invention is to provide a method for processing Aconitum carmichaelii (Linjiang slices). The process of the present invention can expand the medicinal scope and enhance the efficacy of Linjiang slices, solve the problem of insufficient research on the processing mechanism, and at the same time, it is simple to operate and suitable for industrial production.

[0007] The solution of the present invention to the above-mentioned technical problems is as follows:

[0008] A method for processing Aconitum carmichaelii (Linjiang slices) includes the following steps:

[0009] (1) Cleaning: Take the salt-processed Aconitum carmichaelii, place it on the cleaning workbench, screen the salt-processed Aconitum carmichaelii, and transfer it into a clean container for later use. This yields the cleaned salt-processed Aconitum carmichaelii.

[0010] (2) Soaking: Place the cleaned salt-treated aconite obtained in step (1) in a soaking tank and soak it in clean water for 2 to 3 days, changing the water 2 to 3 times a day until the salt is removed. Scrape off the outer skin with a knife, rinse with clean water, and store in a special container for later use to obtain the aconite that has undergone the first soaking.

[0011] (3) Cutting: Adjust the blade of the cutting machine to meet the requirements, and cut the salt-soaked aconite obtained in step (2) into thick slices with a thickness of 3-5 mm to obtain aconite thick slices.

[0012] (4) Soaking: Place the aconite slices obtained in step (3) into a soaking pool and soak them. First soak them in rice water for 1 to 2 days, then soak them in clean water for 2 to 3 days to obtain aconite slices that have been soaked twice.

[0013] (5) Steaming: The thick slices of Aconitum carmichaelii obtained in step (4) after two soakings are spread flat in a wooden steamer and steamed for 6 to 8 hours until the surface is covered with oil to obtain steamed Aconitum carmichaelii.

[0014] (6) Fan drying: Spread the steamed aconite obtained in step (5) on a bamboo sieve, and use a fan with a wind speed of 3-5 m / s to blow air continuously for 10-20 minutes to make its surface cool and dry quickly, thus obtaining the fan-dried aconite.

[0015] (7) Drying: Spread the aconite obtained in step (6) evenly on a stainless steel tray, transfer it to an air source heat pump drying oven, dry for 7-9 hours to dry the moisture of the slices, take it out, and you will get the processed aconite (Linjiang slices) slices.

[0016] The above-mentioned processing method of Aconitum carmichaelii (Linjiang slices) includes step (1), in which the cleaned salt Aconitum carmichaelii medicinal material needs to be manually screened. The screening criteria include: removing impurities such as mud and sand and ash, removing excessive salt frost on the surface, removing rotten, moldy and insect-eaten parts, and removing residual non-medicinal parts.

[0017] The above-mentioned method for processing Aconitum carmichaelii (Linjiang slices) includes step (2), in which Aconitum carmichaelii is soaked according to size, and the water used must be flowing water.

[0018] In the above-mentioned processing method of Aconitum carmichaelii (Linjiang slices), in step (3), the cutting machine is a QYJ2-100 rotary cutting machine, which cuts Aconitum carmichaelii slices of 3-5mm thickness. The thickness is measured by wet slices, and 6 slices are tested every 15 minutes. The error shall not exceed 1mm of the standard. During the cutting process, the shape of the medicinal material slices must be observed at any time, and abnormalities must be screened out.

[0019] The above-mentioned method for processing Aconitum carmichaelii (Linjiang slices) includes step (4), in which Aconitum carmichaelii is soaked according to size, and the water used is running water.

[0020] The above-mentioned method for processing Aconitum carmichaelii (Linjiang slices) includes step (5), in which the thick slices of Aconitum carmichaelii laid flat on the wooden steamer are arranged with the large slices in the center and the small slices around the edges. The steaming process uses a tilting steamer and a fully automatic steam generator, with the temperature not exceeding 120℃, and the degree of steaming of the Aconitum carmichaelii slices is strictly monitored.

[0021] In the above-mentioned processing method of Aconitum carmichaelii (Linjiang slices), in step (6), a fan with a wind speed of 3 to 5 m / s is used to blow air continuously for 10 to 20 minutes until a dense and smooth film structure is formed on the surface of the medicinal material.

[0022] The above-mentioned method for processing Aconitum carmichaelii (Linjiang slices) includes step (7), in which the thickness of the material spread on the stainless steel tray does not exceed 30mm, the temperature of the air source heat pump drying oven is set to 60℃, and the drying time is started after the temperature inside the oven rises to the specified temperature, with the drying endpoint being a moisture content of no more than 13.0%.

[0023] Unless otherwise specified, all percentages mentioned in this invention refer to percentages by mass.

[0024] The present invention also provides a processed Aconitum carmichaelii (Linjiang slices) prepared according to the above-described processing method. The toxic components detected in the processed Aconitum carmichaelii (Linjiang slices) include a decrease in the content of neoaconitine, hypoaconitine, and aconitine, while the content of the effective components, including benzoylneopioidine, benzoylneopioidine, and benzoylhypoaconitine, is increased. The total amount of benzoylneopioidine, benzoylneopioidine, and benzoylhypoaconitine is not less than 0.010%, and the total amount of diester alkaloids, calculated based on the total amount of neoaconitine, hypoaconitine, and aconitine, does not exceed 0.010%, which complies with the provisions of the 2025 edition of the Chinese Pharmacopoeia.

[0025] The present invention provides a method for processing Aconitum carmichaelii (Linjiang slices), which has the following beneficial effects:

[0026] (1) This invention provides a method for processing Aconitum carmichaelii (Linjiang slices). This method uses salted Aconitum carmichaelii as raw material and integrates the essence of traditional Zhangbang craftsmanship (such as soaking in rice water, long-term steaming and fan-drying to form a film) with modern industrial technology, achieving a significant goal of "reducing toxicity and increasing efficacy". The content of diester alkaloids after hydrolysis and conversion is strictly controlled at ≤0.010%, which fully complies with the safety limits of the 2025 edition of the Chinese Pharmacopoeia. On the other hand, the total amount of monoester active ingredients is increased to ≥0.010%, and its cardiotonic and yang-restoring effects are significantly enhanced (the content of benzoyl aconitine and benzoyl aconitine increases by 2 to 3 times or more).

[0027] (2) The process of this invention ensures standardized production flow by precisely quantifying parameters and combining them with automated equipment. It not only inherits the characteristic of "reducing toxicity and increasing efficacy" of the Zhangbang method, but also solves the problems of traditional processing relying on experience and unstable quality. This invention significantly improves the storage stability of medicinal slices (forming a dense film and keratinized texture), and provides key technical support for establishing scientific quality control standards for Linjiang slices, filling the gaps in the pharmacopoeia, and promoting industrial production. Attached Figure Description

[0028] Figure 1 Image of a sample of Aconitum carmichaelii (salted aconite).

[0029] Figure 2 This is a picture of aconite (Linjiang slices) slices.

[0030] Figure 3The characteristic chromatogram of Aconitum carmichaelii (Linjiang slices) is as follows: Peak 1: Neoaconitine (m / z 486); Peak 2 (S1): Songguoling (m / z 358); Peak 3: Fuziling (m / z 454); Peak 4: Neoline (m / z 438); Peak 5: m / z 342; Peak 6 (S2): Benzoyl Neoaconitine (m / z 590); Peak 7: Benzoyl Neoaconitine (m / z 604); Peak 8: Benzoyl Hypoaconitine (m / z 574); Peak 9: m / z 588. Chromatographic column: BEH C18, 100mm × 2.1mm, 1.7μm.

[0031] Figure 4 The image shows the Q-TOF positive ion TIC images of 18 batches of salt-processed Aconitum carmichaelii and Linjiang tablets.

[0032] Figure 5 The results show the determination of toxic and active ingredient content in 18 batches of salted aconite and Linjiang tablets. Detailed Implementation

[0033] To facilitate understanding of the present invention, a more complete description will be given below with reference to the accompanying drawings. Several embodiments of the invention are illustrated in the drawings. However, the invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0035] The sample information used in the embodiments of the present invention is shown in Table 1 below.

[0036] Table 1 Sample Information

[0037]

[0038] Example 1: A method for processing Aconitum carmichaelii (Linjiang slices)

[0039] (1) Cleaning: Take the salt-processed Aconitum carmichaelii medicinal material, place it on the cleaning workbench, manually sift out impurities such as mud and sand, dust and debris, remove excessive salt frost on the surface, remove rotten, moldy and insect-infested parts, remove residual non-medicinal parts, and transfer it into a clean container for later use. The cleaned Aconitum carmichaelii medicinal material is then obtained.

[0040] (2) Soaking: The cleaned salt-treated aconite medicinal materials are sorted by size and placed in a soaking pool with clean water for 2 days. The water is changed 3 times a day until the salt is removed. The outer skin is scraped off with a knife, rinsed with clean water (running water), and stored in a special container for later use. This yields the aconite medicinal materials after the first soaking.

[0041] (3) Cutting: Adjust the blade of the QYJ2-100 rotary herb cutter to be qualified, and cut the salt-soaked aconite into slices with a thickness of 3-5mm (measured by wet slices). Check 6 slices every 15 minutes. The error should not exceed 1mm of the standard. During the cutting process, the shape of the herb slices should be observed at any time, and abnormalities should be removed to obtain thick slices of aconite.

[0042] (4) Soaking: The thick slices of Aconitum carmichaelii are classified by size and placed in a soaking pool. First, they are soaked in rice water for 2 days, and then soaked in clean water (running water) for 2 days to obtain thick slices of Aconitum carmichaelii that have been soaked twice.

[0043] (5) Steaming: The thick slices of Aconitum carmichaelii that have been soaked twice are laid flat in a wooden steamer, with the larger slices in the center and the smaller slices around the edges. The slices are steamed for 7 hours using a tilting steamer and a fully automatic steam generator, with the temperature controlled not to exceed 120°C, until the surface is covered with oil. This is how you get steamed Aconitum carmichaelii.

[0044] (6) Fan drying: After steaming, the aconite is spread flat on a bamboo sieve and blown continuously for 15 minutes with a fan with a wind speed of 4m / s to make its surface cool and dry quickly to form a dense and smooth film structure, thus obtaining the aconite that has been fan dried.

[0045] (7) Drying: Spread the fan-dried aconite evenly on a stainless steel tray, with a thickness not exceeding 30mm. Transfer it to an air source heat pump drying oven, with the temperature set at 60℃. Start timing the drying process after the temperature inside the oven reaches the specified temperature. Dry for 8 hours, until the moisture content of the processed aconite slices does not exceed 13.0%, at which point the drying is complete. Remove the slices to obtain the processed aconite (Linjiang slices).

[0046] The salt-processed Aconitum carmichaelii sample used in Example 1 is as follows: Figure 1 As shown, the processed Aconitum carmichaelii (Linjiang slices) slices prepared according to the method of Example 1 are as follows: Figure 2 As shown.

[0047] Example 2: Mass spectrometry data acquisition of Aconitum carmichaelii (Linjiang slices)

[0048] The determination was performed according to the high performance liquid chromatography-mass spectrometry method (General Chapter 0512 and General Chapter 0431 of the 2025 edition of the Chinese Pharmacopoeia).

[0049] Chromatographic conditions: BEH C18 column (2.1×100mm, 1.7μm; Waters Corporation, USA); mobile phase: acetonitrile (A)-0.1% formic acid aqueous solution (B); gradient elution: 0–11 min, 5%–25% A; 11–15 min, 25%–50% A; 15–16 min, 50%–95% A; flow rate: 0.4 mL / min; injection volume: 1.0 μL; column temperature: 35℃.

[0050] The mass spectrometry conditions were as follows: ion source was a dual-jet electrospray ion source (DualAJS ESI); scanning mode: positive ion mode; mass spectrometry information was acquired using Auto MS / MS acquisition mode; ion scan range: m / z 50~1500, MS / MS m / z 50~1000; curtain gas temperature 320℃; sheath gas temperature 320℃; drying gas volumetric flow rate 8L / min; ionization voltage +3500V / -3200V; capillary voltage 75V; collision energies 20, 40, and 60eV.

[0051] Preparation of reference solutions: Accurately weigh appropriate amounts of benzoylneoprothiolane reference standards, benzoylneoprothiolane reference standards, and benzoylhypoaconitine reference standards. Add isopropanol-dichloromethane (1:1) mixed solution to prepare a stock solution containing 20 μg of benzoylneoprothiolane, 10 μg of benzoylneoprothiolane, and 10 μg of benzoylhypoaconitine per 1 mL. Accurately pipette an appropriate amount of this stock solution and add 50% methanol to prepare a mixed solution containing 200 ng of benzoylneoprothiolane, 100 ng of benzoylneoprothiolane, and 100 ng of benzoylhypoaconitine per 1 mL. Accurately weigh an appropriate amount of sophoridine reference standard and add isopropanol-dichloromethane (1:1) mixed solution to prepare a stock solution containing 8 μg per 1 mL. Accurately pipette an appropriate amount of this stock solution and add 50% methanol to prepare a solution containing 80 ng per 1 mL, which serves as the reference solution.

[0052] Preparation of the test solution: Take about 4.0 g of sample powder (passed through a No. 2 sieve), place it in a stoppered conical flask, add 25 ml of 50% methanol, seal tightly, sonicate (power 250W, frequency 40kHz, water temperature below 25℃) for 30 minutes, cool, shake well, filter, and collect the filtrate to obtain the test solution.

[0053] The determination method involves precisely pipetting 1 μL each of the reference solution and the test solution into a liquid chromatograph-mass spectrometer and measuring the results.

[0054] The characteristic chromatogram of Aconitum carmichaelii (Linjiang slices) obtained in Example 2 is as follows: Figure 3As shown, peak 1: neoaconitine (m / z 486); peak 2 (S1): succinyl aconitine (m / z 358); peak 3: aconitine (m / z 454); peak 4: neorin (m / z 438); peak 5: m / z 342; peak 6 (S2): benzoyl neoaconitine (m / z 590); peak 7: benzoyl aconitine (m / z 604); peak 8: benzoyl hypoaconitine (m / z 574); peak 9: m / z 588. Chromatographic column: BEH C18, 100 mm × 2.1 mm, 1.7 μm.

[0055] The Q-TOF positive ion TIC graphs of 18 batches of salt-processed Aconitum carmichaelii and Linjiang tablets are shown below. Figure 4 As shown. The ion chromatography of the test sample extracted with a mass-to-charge ratio (m / z) should exhibit nine characteristic peaks, among which peaks 2, 6, 7, and 8 should correspond to the retention times of their respective reference peaks. The peak corresponding to the reference peak of sineproterenol is peak S1; calculate the relative retention times of peaks 1-5 with peak S1. The peak corresponding to the reference peak of benzoylneoprothiolane is peak S2; calculate the relative retention time of peak 9 with peak S2. All relative retention times should be within ±10% of the specified values, which are: 0.77 (peak 1), 1.26 (peak 3), 1.38 (peak 4), 1.44 (peak 5), and 1.25 (peak 9).

[0056] Example 3: Determination of the content of toxic and active ingredients in 18 batches of salted aconite and Linjiang slices

[0057] Chromatographic conditions: BEH C18 column (100×2.1mm, 1.7μm; Waters Corporation, USA); mobile phase: methanol (A)-0.1% formic acid aqueous solution (B); gradient elution: 0–1 min, 5%–30% A, 1–2 min, 30%–33% A, 2–3 min, 33%–45% A, 3–10 min, 45%–48% A, 10–10.1 min, 48%–90% A, 10.1–11 min, 90% A, 11–11.5 min, 90%–5% A, 11.5–14 min, 5% A; flow rate: 0.3 mL / min; injection volume: 2.0 μL; column temperature: 35℃.

[0058] The mass spectrometry conditions were as follows: ion source was a dual AJS ESI electrospray ion source; scanning mode: positive ion mode; mass spectrometry information was acquired using Auto MS / MS acquisition mode; ion scan range: m / z 50~1500, MS / MS m / z 50~1000; curtain gas temperature 320℃; sheath gas temperature 320℃; drying gas volumetric flow rate 8L / min; ionization voltage +3500V / -3200V; capillary voltage 75V; collision energies 20, 40, and 60eV.

[0059] Preparation of the reference solution: Accurately weigh appropriate amounts of benzoylneoprothizoline reference standard, benzoylneoprothizoline reference standard, and benzoylhypoaconitine reference standard. Add isopropanol-dichloromethane (1:1) mixed solution to prepare a stock solution containing 19.5412 μg of benzoylneoprothizoline, 9.9009 μg of benzoylneoprothizoline, and 9.8193 μg of benzoylhypoaconitine per 1 mL. Accurately pipette an appropriate amount of this stock solution and add 50% methanol to prepare a mixed solution containing 195.4120 ng of benzoylneoprothizoline, 99.0094 ng of benzoylneoprothizoline, and 98.1930 ng of benzoylhypoaconitine per 1 mL, which serves as the reference solution for the active ingredient. Accurately weigh appropriate amounts of neoaconitine, hypoaconitine, and aconitine reference standards, and prepare a stock solution containing 5.3141 μg of neoaconitine, 5.1386 μg of hypoaconitine, and 5.0959 μg of aconitine per 1 mL by adding isopropanol-dichloromethane (1:1) mixed solution. Accurately pipette an appropriate amount of this stock solution and add 50% methanol to prepare a mixed solution containing 10.6282 ng of neoaconitine, 10.2771 ng of hypoaconitine, and 10.1918 ng of aconitine per 1 mL, which will be used as the reference solution for the toxic components.

[0060] Preparation of the test solution: Accurately weigh 2.0 g of sample powder (passed through a No. 2 sieve) and place it in a stoppered conical flask. Add 3 mL of ammonia test solution and accurately add 50 mL of isopropanol-ethyl acetate (1:1) mixed solution. Weigh the solution and sonicate (300 W power, 40 kHz frequency, water temperature below 25 °C) for 30 minutes. Cool the solution and weigh it again. Make up the weight loss with isopropanol-ethyl acetate (1:1) mixed solution, shake well, and filter. Accurately measure 25 mL of the filtrate, and recover the solvent under reduced pressure below 40 °C until dry. Accurately dissolve the residue in 30% methanol, 50% methanol, and 5 mL of methanol, respectively. Filter the solution and collect the filtrate.

[0061] The determination method involves precisely pipetting 2 μl of each of the above-mentioned reference solution and test solution into a liquid chromatograph-mass spectrometer and measuring the results.

[0062] The results of the determination of toxic and active ingredient contents of 18 batches of salt-processed Aconitum carmichaelii and Linjiang tablets obtained in Example 3 are as follows: Figure 5 As shown. Neoaconitine (C 33 H 45 NO 11 The concentration of aconitine (C) decreased from 0.0300% to undetectable. 33 H 45 NO 10 The concentration of aconitine (C) decreased from 0.0156% to less than one ten-thousandth. 34 H 47 NO 11The percentage of benzoyl aconitine decreased from 0.0064% to less than one ten-thousandth. The percentage of the active ingredient benzoyl neoaconitine increased from 0.0034% in salted aconite to 0.0097%, and the percentage of benzoyl aconitine increased from 0.0004% to 0.0013%, representing increases of 285.97% and 353.59% respectively. The percentage of benzoyl hypoaconitine decreased from 0.0007% to 0.0005%, with the total percentage remaining at no less than 0.001%.

[0063] In summary, the present invention provides a method for processing Aconitum carmichaelii (Linjiang slices), which integrates traditional Zhangbang techniques with modern technology. This method significantly reduces the content of toxic diester alkaloids while substantially increasing the content of effective monoester alkaloids, achieving the goal of "reducing toxicity and increasing efficacy." The processed slices meet the safety standards of the Chinese Pharmacopoeia. The resulting Aconitum carmichaelii (Linjiang slices) have a horny luster, retaining the core medicinal effects of Aconitum carmichaelii in restoring yang, rescuing from collapse, warming the middle jiao, and dispelling cold, while also enhancing storage stability through a "surface-forming" process. This method is the first to quantify the unique Zhangbang techniques, solving the problems of traditional processing relying on experience and experiencing large quality fluctuations. It establishes a standardized production process and quality control system, promoting the scientific inheritance and industrial innovation of traditional processing techniques in Jiangxi.

[0064] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A method for processing Aconitum carmichaelii (Linjiang slices), characterized by comprising the following steps: (1) Cleaning: Take the salt-processed Aconitum carmichaelii, place it on the cleaning workbench, screen the salt-processed Aconitum carmichaelii, and transfer it into a clean container for later use. This yields the cleaned salt-processed Aconitum carmichaelii. (2) Soaking: Place the cleaned salt-treated aconite obtained in step (1) in a soaking tank and soak it in clean water for 2 to 3 days, changing the water 2 to 3 times a day until the salt is removed. Scrape off the outer skin with a knife, rinse with clean water, and store in a special container for later use to obtain the aconite that has undergone the first soaking. (3) Cutting: Adjust the blade of the cutting machine to meet the requirements, and cut the salt-soaked aconite obtained in step (2) into thick slices with a thickness of 3-5 mm to obtain aconite thick slices. (4) Soaking: Place the aconite slices obtained in step (3) into a soaking pool and soak them. First soak them in rice water for 1 to 2 days, then soak them in clean water for 2 to 3 days to obtain aconite slices that have been soaked twice. (5) Steaming: The thick slices of Aconitum carmichaelii obtained in step (4) after two soakings are spread flat in a wooden steamer and steamed for 6 to 8 hours until the surface is covered with oil to obtain steamed Aconitum carmichaelii. (6) Fan drying: Spread the steamed aconite obtained in step (5) on a bamboo sieve, and use a fan with a wind speed of 3-5 m / s to blow air continuously for 10-20 minutes to make its surface cool and dry quickly, thus obtaining the fan-dried aconite. (7) Drying: Spread the aconite obtained in step (6) evenly on a stainless steel tray, transfer it to an air source heat pump drying oven, dry for 7-9 hours to dry the moisture of the slices, take it out, and you will get the processed aconite (Linjiang slices) slices.

2. The method for processing Aconitum carmichaelii (Linjiang slices) according to claim 1, characterized in that, In step (1), the cleaned salt-processed Aconitum carmichaelii medicinal material needs to be manually screened. The screening criteria include: removing impurities such as mud and sand, dust and debris, removing excessive salt frost on the surface, removing rotten, moldy and insect-infested parts, and removing residual non-medicinal parts.

3. The method for processing Aconitum carmichaelii (Linjiang slices) according to claim 1, characterized in that, In step (2), the aconite root needs to be soaked and rinsed according to its size, and the water used must be running water.

4. The method for processing Aconitum carmichaelii (Linjiang slices) according to claim 1, characterized in that, In step (3), the medicine cutting machine is a QYJ2-100 rotary medicine cutting machine, which cuts aconite slices of 3-5mm thickness. The thickness is measured by wet slices, and 6 slices are tested every 15 minutes. The error must not exceed 1mm of the standard. During the cutting process, the shape of the medicinal material slices must be observed at any time, and abnormalities must be screened out.

5. The method for processing Aconitum carmichaelii (Linjiang slices) according to claim 1, characterized in that, In step (4), the aconite root needs to be soaked and rinsed according to its size, and the water used must be running water.

6. The method for processing Aconitum carmichaelii (Linjiang slices) according to claim 1, characterized in that, In step (5), the thick slices of Aconitum carmichaelii laid flat on the wooden steamer should be arranged with the larger slices in the center and the smaller slices around the edges. The steaming process uses a tilting steamer and a fully automatic steam generator, with the temperature not exceeding 120℃. The degree of steaming of the Aconitum carmichaelii slices during the steaming process is strictly monitored.

7. The method for processing Aconitum carmichaelii (Linjiang slices) according to claim 1, characterized in that, In step (6), a fan with a wind speed of 3 to 5 m / s is used to blow air continuously for 10 to 20 minutes until a dense and smooth film structure is formed on the surface of the medicinal material.

8. The method for processing Aconitum carmichaelii (Linjiang slices) according to claim 1, characterized in that, In step (7), the thickness of the material on the stainless steel tray shall not exceed 30 mm, the temperature of the air source heat pump drying oven shall be set to 60°C, and the drying time shall be started after the temperature inside the oven rises to the specified temperature, with the drying endpoint being a moisture content of no more than 13.0%.

9. A processed herbal slice of Aconitum carmichaelii (Linjiang slices) prepared using the processing method described in any one of claims 1 to 8, characterized in that, The content of toxic components, including neoaconitine, hypoaconitine, and aconitine, in the tested Aconitum carmichaelii (Linjiang slices) was reduced, while the content of effective components, including benzoylneoaconitine, benzoylneoaconitine, and benzoyl hypoaconitine, was increased. The total amount of benzoylneoaconitine, benzoylneoaconitine, and benzoyl hypoaconitine was not less than 0.010%, and the total amount of diester alkaloids, calculated as neoaconitine, hypoaconitine, and aconitine, did not exceed 0.010%, which complies with the provisions of the 2025 edition of the Chinese Pharmacopoeia.

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