Positive pressure column passing equipment

Abstract

The embodiment of the invention provides positive-pressure column passing equipment. The positive-pressure column passing equipment comprises a seat body, a pore plate and a positive-pressure assembly, the positive pressure assembly is provided with a gas inlet and a plurality of gas inlet units, each gas inlet unit comprises a gas outlet, a gas inlet cavity and a gas outlet cavity, the gas inlet cavity is communicated with the gas inlet, the gas outlet cavity is communicated with the gas outlet, and the gas outlet corresponds to the mounting hole and is used for allowing gas to enter the solid-phase extraction column. The air inlet unit has a communicating state and a separating state; the gas inlet unit correspondingly abuts against the solid-phase extraction column, so that the gas inlet cavity communicates with the gas outlet cavity, a gas flow path sequentially passing through the gas inlet, the gas inlet cavity, the gas outlet cavity, the gas outlet and the solid-phase extraction column is formed, and the gas inlet unit is in a communicating state; when the gas inlet unit is separated from the solid-phase extraction column, the gas inlet cavity and the gas outlet cavity are separated to cut off the gas flow path, and the gas inlet unit is in a separated state. The positive pressure column passing equipment provided by the embodiment of the invention can reduce the risk of gas leakage.

Description

Technical Field

[0001] This application relates to the field of positive pressure column technology, and in particular to a positive pressure column device. Background Technology

[0002] In related technologies, positive pressure column chromatography equipment achieves sealing through the overall deformation of the end face of a compressible material under stress, and then gas is introduced. When a solid phase extraction (SPE) column is missing from the well plate (e.g., a 96-well plate has only 95 SPE columns), the positive pressure column chromatography equipment may not be completely sealed in some areas, which can easily cause gas leakage, resulting in insufficient extraction by the SPE column within the set time and pressure. Summary of the Invention

[0003] In view of this, the main objective of the embodiments of this application is to provide a positive pressure column device that can reduce the risk of gas leakage.

[0004] To achieve the above objectives, the technical solution of this application embodiment is implemented as follows: This application provides a positive pressure column device, including a base, an orifice plate, and a positive pressure assembly; The perforated plate is disposed on the base and has multiple mounting holes for accommodating a solid phase extraction column. The positive pressure component is disposed on the base and spaced apart from the base to form a positive pressure space, the positive pressure space being used to accommodate the orifice plate; the positive pressure component has an air inlet and multiple air inlet units, each air inlet unit including an air outlet, an air inlet chamber and an air outlet chamber, the air inlet chamber communicating with the air inlet, the air outlet chamber communicating with the air outlet, and the air outlet corresponding to the mounting hole for supplying gas into the solid phase extraction column; The air intake unit has a connected state and a separated state; the air intake unit connects with the solid phase extraction column to form an airflow path that sequentially passes through the air inlet, the air intake chamber, the air outlet chamber, the air outlet, and the solid phase extraction column, and the air intake unit is in the connected state; when the air intake unit separates from the solid phase extraction column, the air intake chamber and the air outlet chamber are separated to block the airflow path, and the air intake unit is in the separated state.

[0005] In one embodiment, the positive pressure assembly includes a positive pressure seat having multiple mounting spaces, a portion of which forms the air intake chamber. The air intake unit includes a valve shaft, which is movably disposed in the mounting space to form the air outlet chamber with the positive pressure seat within the mounting space. The valve shaft switches between the connected state and the separated state by contacting and separating from the solid phase extraction column.

[0006] In one embodiment, the valve shaft has an abutment end at one end away from the air inlet chamber, the abutment end extending out of the mounting space for contacting the solid phase extraction column, the valve shaft having a flow channel extending in the extension direction, the abutment end having the air outlet, and the opposite ends of the flow channel communicating with the air outlet chamber and the air outlet, respectively.

[0007] In one embodiment, the valve shaft has an extension end, a valve stem section, and an abutment end, the valve stem section being located between the extension end and the abutment end, another portion of the mounting space forming a mounting channel, the valve shaft being movably inserted into the mounting channel, and a portion of the outer wall of the valve stem section being recessed to form the air outlet chamber spaced apart from the wall of the mounting channel, the extension end extending into the air inlet chamber.

[0008] In one embodiment, when the abutting end separates from the solid-phase extraction column, at least one of the extending end and the valve stem section blocks the installation channel to separate the air inlet chamber and the air outlet chamber; when the abutting end abuts against the solid-phase extraction column, the valve shaft moves toward the side closer to the air inlet chamber to connect the air inlet chamber and the air outlet chamber.

[0009] In one embodiment, the cross-sectional dimension of the air intake chamber is larger than the cross-sectional dimension of the mounting channel to form a first limiting step at the connection; the cross-sectional dimension of the extended end is larger than the cross-sectional dimension of the valve stem section to form a second limiting step at the connection; when the air intake unit is in the separated state, the first limiting step and the second limiting step are correspondingly engaged; when the air intake unit is in the connected state, the first limiting step and the second limiting step are separated from each other.

[0010] In one embodiment, the positive pressure assembly further includes a first seal, which is disposed at one end of the mounting channel near the air inlet chamber and located on the side of the air outlet chamber near the air inlet chamber.

[0011] In one embodiment, the positive pressure assembly further includes a second seal, which is disposed at one end of the mounting channel away from the air inlet chamber and located on the side of the air outlet chamber away from the air inlet chamber.

[0012] In one embodiment, the positive pressure assembly further includes a third seal, which blocks the opening of the solid phase extraction column when the abutting end abuts against the solid phase extraction column, and the third seal is disposed at the junction of the abutting end and the solid phase extraction column.

[0013] In one embodiment, a portion of the outer surface of the abutting end protrudes to form a protrusion, the protrusion being used to abut against the outer end face of the solid phase extraction column; the positive pressure assembly further includes a first elastic element, the first elastic element being sleeved on the valve shaft, and its opposite ends abutting against the protrusion and the positive pressure seat, respectively.

[0014] In one embodiment, the positive pressure assembly further includes a second elastic element disposed within the air intake chamber, with its opposite ends abutting against the extension end and the positive pressure seat, respectively.

[0015] In one embodiment, the positive pressure assembly further includes a baffle, a guide post, and a third elastic element. The guide post is disposed on the side of the positive pressure seat near the orifice plate. The baffle is slidably sleeved on the guide post. The third elastic element is sleeved on the guide post and abuts against the baffle and the positive pressure seat respectively. The baffle has multiple through holes. The end of the valve shaft opposite to the air inlet chamber has an abutment end. The abutment ends are correspondingly inserted into the through holes. The baffle is used to abut against the outer end face of the solid phase extraction column.

[0016] In one embodiment, the lower end face of the abutting end is located within the through hole.

[0017] In one embodiment, the positive pressure column device further includes a temperature control component, which is vertically and flexibly mounted on the base. The temperature control component has a downward-facing temperature control cavity, and the temperature control component switches the orifice plate between entering and exiting the temperature control cavity by raising and lowering itself.

[0018] In one embodiment, the temperature control component includes a silicone heating pad, a heat sink, and a temperature control base. The temperature control base has a temperature control cavity, and the silicone heating pad and the heat sink are disposed within the temperature control cavity. The silicone heating pad is disposed on the side of the heat sink away from the perforated plate.

[0019] In one embodiment, the positive pressure column device includes a lifting assembly and a translation assembly. The lifting assembly is disposed on the base body, and the temperature control assembly and the positive pressure assembly are disposed on the lifting assembly along a first direction to move relative to the base body in a vertical direction via the lifting assembly. The translation assembly is slidably disposed on the base body along the first direction, and the perforated plate is disposed on the translation assembly to move relative to the base body via the translation assembly.

[0020] In one embodiment, the positive pressure column-passing device includes a lifting assembly, which includes an upper support plate, a lifting support plate, a synchronous pulley structure, a drive motor, a ball screw nut, and a ball screw. The ball screw is rotatably mounted on the base, the upper support plate is sleeved on the ball screw, and the lifting support plate is sleeved on the ball screw via the ball screw nut. The positive pressure assembly is mounted on the lifting support plate, and the drive motor and the synchronous pulley structure are mounted on the upper support plate. The drive motor is driven to the ball screw via the synchronous pulley structure, so that by driving the ball screw to rotate, the lifting support plate drives the positive pressure assembly to rise and fall.

[0021] This application provides a positive pressure column feed device, which includes a base, an orifice plate, and a positive pressure assembly. The air inlet chamber of the positive pressure assembly is connected to the air inlet, and the air outlet chamber is connected to the air outlet. The air inlet unit has a connected state and a separated state; the air inlet unit connects the air inlet chamber and the air outlet chamber by correspondingly abutting against the solid-phase extraction column, forming an airflow path that sequentially passes through the air inlet, air inlet chamber, air outlet chamber, air outlet, and solid-phase extraction column, and the air inlet unit is in the connected state. When the air inlet unit is separated from the solid-phase extraction column, the air inlet chamber and the air outlet chamber are separated to block the airflow path, and the air inlet unit is in the separated state. That is to say, the air inlet unit of this application can achieve the separation and connection of the air inlet chamber and the air outlet chamber by switching between separation and contact with the solid-phase extraction column. Therefore, when there are missing solid-phase extraction columns on the orifice plate (i.e., when some mounting holes are not equipped with solid-phase extraction columns), only the air inlet unit corresponding to the solid-phase extraction column will be in the connected state, and can normally supply gas into the solid-phase extraction column. The inlet unit, which is not in contact with the solid-phase extraction column (i.e., separated from the solid-phase extraction column), is in a separated state, with no communication between its inlet and outlet chambers. Therefore, gas from the positive pressure column equipment will not leak from this inlet unit, reducing the risk of gas leakage and allowing for more complete extraction within the set time and pressure. Simultaneously, it allows operators to flexibly arrange the number of solid-phase extraction columns on the orifice plate; the number of columns in a single test does not need to strictly correspond to the mounting holes, improving the operational convenience of the positive pressure column equipment. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the structure of a positive pressure column device according to an embodiment of this application; Figure 2 for Figure 1 A cross-sectional view of the positive pressure column equipment along the first direction; Figure 3 for Figure 1 A structural schematic diagram of the positive pressure column equipment from another perspective; Figure 4 for Figure 1 A schematic diagram showing the fit between the mesoporous plate and the solid-phase extraction column; Figure 5 for Figure 2 Sectional view along line AA; Figure 6 for Figure 5 A magnified view of a section at point B in the middle; Figure 7 for Figure 6 A schematic diagram showing the relationship between the air intake unit, baffle, and solid-phase extraction column; in the diagram, the air intake unit and baffle are separated from the solid-phase extraction column; Figure 8 for Figure 6 A schematic diagram showing the relationship between the intake unit, baffle, and solid-phase extraction column; in the diagram, the intake unit is separated from the solid-phase extraction column, and the baffle is in contact with the solid-phase extraction column; Figure 9 for Figure 6 A schematic diagram showing the relationship between the intake unit, baffle, and solid-phase extraction column; in the diagram, the intake unit abuts against the solid-phase extraction column, the baffle abuts against the solid-phase extraction column, and the intake chamber and outlet chamber are separated. Figure 10 for Figure 6 A schematic diagram showing the relationship between the intake unit, baffle, and solid-phase extraction column; in the diagram, the intake unit abuts against the solid-phase extraction column, the baffle abuts against the solid-phase extraction column, and the intake chamber and the outlet chamber are connected. Figure 11 for Figure 6 A schematic diagram of the structure when the central air intake unit is not in contact with the solid phase extraction column; Figure 12 for Figure 6 A partial structural diagram of the positive pressure seat; Figure 13 for Figure 2 C-axis sectional view; Figure 14 for Figure 13 A schematic diagram showing the relationship between the orifice plate, solid-phase extraction column, and temperature control components. The orifice plate and solid-phase extraction column are located inside the temperature control chamber.

[0023] Explanation of reference numerals in the attached figures 10. Seat; 20. Orifice plate; 20a. Mounting hole; 30. Positive pressure assembly; 30a. Positive pressure space; 30b. Air inlet; 31. Air inlet unit; 31a. Air outlet; 31b. Air inlet chamber; 31c. Air outlet chamber; 311. Valve shaft; 311a. Flow passage; 312. Abutment end; 3121. Protrusion; 313. Extension end; 314. Valve stem section; 315. Second limiting step; 32. Positive pressure seat; 32a. Mounting space; 32b. Mounting channel; 321. First limiting step; 33. First seal; 331. Second seal; 332. Third seal; 34. First elastic element; 341. Third elastic element. Components; 35, baffle; 35a, through hole; 36, guide post; 40, solid phase extraction column; 50, temperature control assembly; 50a, temperature control chamber; 51, silicone heating pad; 52, heat sink; 53, temperature control base; 60, lifting assembly; 61, upper support plate; 62, lifting support plate; 63, synchronous belt pulley structure; 631, synchronous belt pulley; 632, synchronous belt; 633, tensioning pulley; 634, idler pulley; 64, drive motor; 65, ball screw nut; 66, ball screw; 70, translation assembly; 71, linear guide rail; 72, screw motor; 73, screw nut; 74, slide adapter; 75, bearing seat; 76, motor seat. Detailed Implementation

[0024] In this application, the orientation or positional relationship of "vertical direction" and "first direction" is based on the appendix. Figure 2 The orientation or positional relationship shown is for illustrative purposes only and is not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this application.

[0025] In the description of the embodiments of this application, technical terms such as "first," "second," and "third" are used only to distinguish different objects and should not be construed as indicating or implying relative importance or implicitly specifying the number, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of this application, "multiple" means two or more, unless otherwise explicitly defined.

[0026] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0027] One embodiment of this application provides a positive pressure column conveying device; please refer to [link / reference]. Figures 1 to 3The positive pressure column device includes a base 10, an orifice plate 20, and a positive pressure component 30.

[0028] Please see Figure 4 The orifice plate 20 is disposed on the base 10 and has multiple mounting holes 20a, which are used to accommodate the solid phase extraction column 40.

[0029] The positive pressure component 30 is disposed on the base 10 and forms a positive pressure space 30a with the base 10 at a distance. The positive pressure space 30a is used to accommodate the orifice plate 20. The positive pressure component 30 has an air inlet 30b and a plurality of air inlet units 31. The air inlet unit 31 includes an air outlet 31a, an air inlet chamber 31b and an air outlet chamber 31c. The air inlet chamber 31b is connected to the air inlet 30b, and the air outlet chamber 31c is connected to the air outlet 31a. The air outlet 31a corresponds to the mounting hole 20a for supplying gas into the solid phase extraction column 40.

[0030] Please see Figures 5 to 11 The intake unit 31 has a connected state and a separated state. The intake unit 31 connects with the solid phase extraction column 40 to form an airflow path that passes through the intake port 30b, intake chamber 31b, exhaust chamber 31c, exhaust port 31a and solid phase extraction column 40 in sequence. The intake unit 31 is in the connected state. When the intake unit 31 is separated from the solid phase extraction column 40, the intake chamber 31b and exhaust chamber 31c are separated to cut off the airflow path. The intake unit 31 is in the separated state.

[0031] Specifically, positive pressure column chromatography equipment can be used for processing various types of samples. For example, it is used to pretreat biological samples before mass spectrometry analysis. Pretreatment of biological samples includes protein extraction, protein reduction, alkylation, enzymatic digestion, and removal of interfering substances such as salts, lipids, and nucleic acids. This purifies proteins or yields clean peptides after enzymatic digestion, thereby improving the signal-to-noise ratio, the number of proteins identified, and the accuracy of quantification in mass spectrometry analysis. Furthermore, high-precision temperature control is required during pretreatment to ensure the stable and smooth execution of biochemical reactions such as protein reduction, alkylation, and enzymatic digestion. This application describes the use of positive pressure column chromatography equipment for protein sample pretreatment as an example.

[0032] The solid-phase extraction column 40 is installed in the mounting hole 20a of the well plate 20 and is used to contain the protein sample.

[0033] It should be noted that the well plate 20 has multiple mounting holes 20a, the number of which is unlimited, such as 96 mounting holes 20a. Each mounting hole 20a can have a corresponding solid-phase extraction column 40 installed. Depending on actual needs, some mounting holes 20a can have one solid-phase extraction column 40 installed, while others can remain uninstalled. This allows operators to flexibly arrange the solid-phase extraction columns 40, without requiring a direct correspondence between the number of columns and mounting holes 20a in a single experiment, and the installation positions of the columns can be randomly set. For example, the solid-phase extraction columns 40 can be arranged at intervals on the well plate 20.

[0034] The positive pressure assembly 30 is used to perform positive pressure column permeation of the solid-phase extraction column 40. Using positive pressure column permeation for protein sample pretreatment facilitates the integration of the positive pressure column permeation equipment into automated pipetting workstations, allowing for flexible configuration. Compared to centrifugal column permeation, it does not require highly integrated equipment and is relatively smaller in size.

[0035] The space between the positive pressure component 30 and the base 10 is a positive pressure space 30a, which is used for the orifice plate 20 to enter and perform positive pressure column operation.

[0036] The air inlet 30b of the positive pressure component 30 is used to supply gas.

[0037] The positive pressure assembly 30 has multiple air intake units 31, each corresponding to a solid-phase extraction column 40 on a mounting hole 20a. However, depending on actual needs, each air intake unit 31 can correspond to a solid-phase extraction column 40. Alternatively, when some mounting holes 20a do not have a solid-phase extraction column 40, only some air intake units 31 may have one corresponding solid-phase extraction column 40, while other air intake units 31 may not have one.

[0038] It should be noted that the air intake unit 31 is a unit within the positive pressure assembly 30 used to supply gas from the air intake port 30b into the solid phase extraction column 40.

[0039] Each air intake unit 31 includes an air outlet 31a, an air intake chamber 31b communicating with the air intake 30b, and an air outlet chamber 31c communicating with the air outlet 31a. The positions of the air outlets 31a and the mounting holes 20a are one-to-one. When a solid phase extraction column 40 is installed on the mounting hole 20a at the corresponding position, the air outlet 31a communicates with the opening of the solid phase extraction column 40 to allow gas to enter the solid phase extraction column 40.

[0040] It should be noted that the air inlet chamber 31b and the air outlet chamber 31c have two states: connected and separated. The switching between the two states is determined by whether the air inlet unit 31 is in contact with the solid phase extraction column 40.

[0041] Specifically, when a solid-phase extraction column 40 is installed on the mounting hole 20a, the air intake unit 31 corresponding to the position of the mounting hole 20a abuts against the solid-phase extraction column 40, thereby connecting the air intake chamber 31b and the air outlet chamber 31c of the air intake unit 31. Thus, the air inlet 30b, air intake chamber 31b, air outlet chamber 31c, and air outlet 31a are sequentially connected, and the gas flowing in from the air inlet 30b can flow sequentially along the above-mentioned chambers and then enter the solid-phase extraction column 40, thereby forming an airflow path that sequentially passes through the air inlet 30b, air intake chamber 31b, air outlet chamber 31c, air outlet 31a, and solid-phase extraction column 40.

[0042] When the solid-phase extraction column 40 is not installed on the mounting hole 20a, or when the solid-phase extraction column 40 is installed on the mounting hole 20a but not in contact with the corresponding air intake unit 31, the air intake unit 31 and the solid-phase extraction column 40 are separated, thus separating the air intake chamber 31b and the air outlet chamber 31c of the air intake unit 31, and the two are not connected. Gas cannot flow through the air intake port 30b and the air intake chamber 31b to the air outlet chamber 31c, so no gas leakage will occur, and the aforementioned airflow path is blocked.

[0043] In the positive pressure column extraction device of this application embodiment, the air inlet chamber 31b of the positive pressure component 30 is connected to the air inlet 30b, and the air outlet chamber 31c is connected to the air outlet 31a. The air inlet unit 31 has a connected state and a separated state; the air inlet unit 31 connects the air inlet chamber 31b and the air outlet chamber 31c by correspondingly abutting against the solid phase extraction column 40, and forms an airflow path that sequentially passes through the air inlet 30b, the air inlet chamber 31b, the air outlet chamber 31c, the air outlet 31a, and the solid phase extraction column 40, and the air inlet unit 31 is in the connected state. When the air inlet unit 31 is separated from the solid phase extraction column 40, the air inlet chamber 31b and the air outlet chamber 31c are separated to cut off the airflow path, and the air inlet unit 31 is in the separated state. That is to say, the air inlet unit 31 of this application can realize the separation and connection of the air inlet chamber 31b and the air outlet chamber 31c by switching between separation and contact with the solid phase extraction column 40. Therefore, when a solid-phase extraction column 40 is missing from the orifice plate 20 (i.e., when some mounting holes 20a are not equipped with a solid-phase extraction column 40), only the air intake unit 31 that is in contact with the solid-phase extraction column 40 will be in a connected state and can supply gas to the solid-phase extraction column 40 normally. The air intake unit 31 that is not in contact with the solid-phase extraction column 40 (i.e., separated from the solid-phase extraction column 40) will be in a separated state, and the air intake chamber 31b and the air outlet chamber 31c of this air intake unit 31 will not be connected. Thus, the gas in the positive pressure column device will not leak from the air intake unit 31, thereby reducing the risk of gas leakage and allowing the solid-phase extraction column 40 to extract more completely within the set time and pressure. Furthermore, it also prevents gas leakage caused by inconsistent stress heights of some solid-phase extraction columns 40, as is present in related technologies. At the same time, it allows operators to flexibly arrange the number of solid phase extraction columns 40 on the orifice plate 20. The number of solid phase extraction columns 40 in a single test does not need to strictly correspond to the installation hole 20a, which can improve the ease of operation of the positive pressure column equipment.

[0044] In one embodiment, please refer to Figures 6 to 11 The positive pressure assembly 30 includes a positive pressure base 32 with multiple mounting spaces 32a. A portion of the mounting space 32a forms an air intake chamber 31b. The air intake unit 31 includes a valve shaft 311, which is movably disposed in the mounting space 32a to form an air outlet chamber 31c with the positive pressure base 32 within the mounting space 32a. The valve shaft 311 switches between a connected state and a separated state by contacting and separating from the solid phase extraction column 40. In other words, by using the movable valve shaft 311, the connection and separation of the air intake chamber 31b and the air outlet chamber 31c can be effectively achieved, thereby enabling the state switching of the air intake unit 31.

[0045] Specifically, the positive pressure seat 32 has multiple mounting spaces 32a inside, and each mounting space 32a corresponds to a valve shaft 311. In fact, for the positive pressure assembly 30, each mounting space 32a and the corresponding valve shaft 311 form at least a part of an intake unit 31.

[0046] Within the installation space 32a, a portion forms an intake chamber 31b, and another portion (the area between the valve shaft 311 and the positive pressure seat 32) forms an outlet chamber 31c. The valve shaft 311 is disposed within the installation space 32a and is movable relative to the positive pressure seat 32.

[0047] When the valve shaft 311 is not in contact with the solid-phase extraction column 40 (i.e., the two are separated), the valve shaft 311 is in an initial position that allows the intake chamber 31b and the outlet chamber 31c to be separated (not connected), and the intake unit 31 is in a separated state. However, when the valve shaft 311 gradually comes into contact with the solid-phase extraction column 40, the valve shaft 311 moves relative to the positive pressure seat 32 under the action of the solid-phase extraction column 40, thereby allowing the intake chamber 31b and the outlet chamber 31c to be connected, and the intake unit 31 is in a connected state.

[0048] It should be noted that the specific formation method of the airflow path within the positive pressure component 30 can be set according to the actual situation.

[0049] For example, please see Figures 7 to 11 The valve shaft 311 has an abutment end 312 at the end opposite to the air inlet chamber 31b. The abutment end 312 extends into an installation space 32a for contacting the solid-phase extraction column 40. The valve shaft 311 has a flow passage 311a extending in the extending direction, and the abutment end 312 has an air outlet 31a. The two opposite ends of the flow passage 311a are respectively connected to the air outlet chamber 31c and the air outlet 31a. Thus, by allowing flow through the inside of the valve shaft 311, the sealing of the airflow path can be improved, and it can be better facilitated to allow air to enter the solid-phase extraction column 40.

[0050] Specifically, the valve shaft 311 abuts against the solid phase extraction column 40 through the abutment end 312 extending out of the installation space 32a, and communicates with the solid phase extraction column 40 through the air outlet 31a of the abutment end 312.

[0051] The valve shaft 311 is hollow, so that a flow passage 311a extending axially along the valve shaft 311 is formed inside. The flow passage 311a connects the outlet chamber 31c and the outlet 31a, thereby preventing gas leakage from the outlet chamber 31c to the outlet 31a.

[0052] In one specific embodiment, please refer to Figures 7 to 11The valve shaft 311 has an extension end 313, a valve stem section 314, and an abutment end 312. The valve stem section 314 is located between the extension end 313 and the abutment end 312, and the outlet chamber 31c is located on the side of the valve stem section 314 closer to the extension end 313. This significantly extends the length of the flow passage 311a within the valve shaft 311, thereby greatly enhancing the sealing performance of the gas flow path.

[0053] Of course, in other embodiments, air can also be introduced from the outside through the valve shaft 311.

[0054] For example, the installation space 32a includes an installation channel 32b communicating with the intake chamber 31b. The installation channel 32b has an installation port. The valve shaft 311 is movably disposed within the installation channel 32b and forms an outlet chamber 31c and a flow passage 311a with the wall of the installation channel 32b at intervals. The opening size of the installation port is larger than the cross-sectional size of the valve shaft 311 to form the outlet 31a at intervals. The outlet chamber 31c communicates with the outlet 31a through the flow passage 311a. Thus, by using a method of air passing through the outside of the valve shaft 311, it is not necessary to machine the flow passage 311a inside the valve shaft 311.

[0055] In one embodiment, please refer to Figures 7 to 11 The valve shaft 311 has an extension end 313, a valve stem section 314, and an abutment end 312. The valve stem section 314 is located between the extension end 313 and the abutment end 312. Another part of the mounting space 32a forms a mounting channel 32b. The valve shaft 311 is movably inserted into the mounting channel 32b, and a portion of the outer wall of the valve stem section 314 is recessed to form an exhaust chamber 31c spaced apart from the wall of the mounting channel 32b. The extension end 313 extends into the intake chamber 31b. Thus, the intake chamber 31b and the exhaust chamber 31c can be connected by the movement of the valve shaft 311, thereby enabling the switching of the state of the intake unit 31.

[0056] Specifically, the extension end 313 of the valve shaft 311 is the end of the valve shaft 311 that is away from the abutment end 312, and it extends into the air intake chamber 31b.

[0057] The valve stem section 314 of the valve shaft 311 is a shaft region located between the extension end 313 and the abutment end 312, with its opposite ends connected to the extension end 313 and the abutment end 312, respectively.

[0058] The specific structure of valve shaft 311 can be set according to the actual situation.

[0059] For example, the insertion end 313 of the valve shaft 311 is a screw, which is fastened to the end of the valve stem section 314 away from the abutment end 312.

[0060] The valve shaft 311 is movable relative to the mounting channel 32b. In fact, the valve stem section 314 of the valve shaft 311 is located in the mounting channel 32b. Since a part of the outer wall of the valve stem section 314 is recessed relative to other parts, a gap space is formed between this part and the mounting channel 32b, which is the air outlet chamber 31c.

[0061] When the intake unit 31 is in the separated state, this space (i.e., the outlet chamber 31c) is not connected to the intake chamber 31b on the side of the extension end 313. As the valve shaft 311 moves toward the side closer to the intake chamber 31b, this space (i.e., the outlet chamber 31c) will also move toward the side closer to the intake chamber 31b until it is connected to the intake chamber 31b.

[0062] It should be noted that the shape of the recessed area on the outer wall of the valve stem section 314 and the connection method with the air outlet 31a can be set according to the actual situation.

[0063] For example, in the recessed area of ​​the outer wall of the valve stem section 314, the depth of the recess first increases and then decreases from top to bottom. The connection between the flow passage 311a and the intake chamber 31b is located at the maximum recessed depth of the recessed area, thereby improving the airflow effect.

[0064] In one embodiment, please refer to Figure 7 and Figure 8 When the contact end 312 separates from the solid-phase extraction column 40, at least one of the extension end 313 and the valve stem section 314 blocks the installation channel 32b, thereby separating the inlet chamber 31b and the outlet chamber 31c. When the contact end 312 abuts against the solid-phase extraction column 40, the valve shaft 311 moves towards the side closer to the inlet chamber 31b, thereby connecting the inlet chamber 31b and the outlet chamber 31c. Thus, the separation and connection between the inlet chamber 31b and the outlet chamber 31c can be effectively achieved.

[0065] Specifically, the valve shaft 311 can block the mounting channel 32b through the insertion end 313. For example, the cross-sectional dimension of the insertion end 313 is larger than the cross-sectional dimension of the mounting channel 32b, and the mounting channel 32b is blocked by the insertion end 313 fitting against the end face of the mounting channel 32b.

[0066] Depending on the actual situation, the valve shaft 311 can also block the installation channel 32b through the valve stem section 314. For example, the cross-sectional dimensions of the area where the valve stem section 314 connects with the extension end 313 are the same as the cross-sectional dimensions of the installation channel 32b, or the area where the valve stem section 314 connects with the extension end 313 is interference-fitted with the installation channel 32b to achieve the blocking of the installation channel 32b.

[0067] Of course, the valve shaft 311 can also block the installation channel 32b by the extension end 313 and the valve stem section 314 together.

[0068] When the contact end 312 separates from the solid-phase extraction column 40, the inlet chamber 31b and the outlet chamber 31c are separated and not connected due to the blocking effect of at least one of the extension end 313 and the valve stem section 314. When the contact end 312 abuts against the solid-phase extraction column 40, the valve shaft 311 moves towards the side closer to the inlet chamber 31b under the abutment action of the solid-phase extraction column 40, causing the outlet chamber 31c to gradually approach the inlet chamber 31b until the two are connected.

[0069] In one embodiment, please refer to Figure 10 and Figure 12 The cross-sectional dimension of the intake chamber 31b is larger than that of the mounting channel 32b, forming a first limiting step 321 at the connection point; the cross-sectional dimension of the extension end 313 is larger than that of the valve stem section 314, forming a second limiting step 315 at the connection point; when the intake unit 31 is in the separated state, the first limiting step 321 and the second limiting step 315 are correspondingly engaged; when the intake unit 31 is in the connected state, the first limiting step 321 and the second limiting step 315 are separated from each other. Therefore, on the one hand, the sealing effect on the mounting channel 32b can be greatly improved, and on the other hand, the valve shaft 311 can be prevented from detaching from the mounting channel 32b.

[0070] Specifically, the installation space 32a of the positive pressure seat 32 includes an air intake chamber 31b and an installation channel 32b, one end of which is connected to the air intake chamber 31b and the other end is open.

[0071] Because the cross-sectional dimension of the intake chamber 31b is larger than that of the mounting channel 32b, a first limiting step 321 can be formed at the point where the two are connected. At the same time, the cross-sectional dimension of the extension end 313 located in the intake chamber 31b is larger than that of the valve stem section 314 located in the mounting channel 32b, thus a second limiting step 315 can be formed at the point where the two are connected.

[0072] It is understandable that when the contact end 312 is separated from the solid phase extraction column 40, that is, when the air intake unit 31 is in the separated state, the first limiting step 321 and the second limiting step 315 are in corresponding contact, thereby blocking the connection between the installation channel 32b and the air intake chamber 31b, and preventing the valve shaft 311 from disengaging from the positive pressure seat 32.

[0073] When the contact end 312 contacts the solid phase extraction column 40, that is, when the air intake unit 31 is in the separated state, the first limiting step 321 and the second limiting step 315 separate from each other, thus enabling the air intake chamber 31b and the air outlet chamber 31c to be connected.

[0074] In one embodiment, please refer to Figures 6 to 11 The positive pressure assembly 30 also includes a first seal 33, which is disposed at one end of the mounting channel 32b near the inlet chamber 31b and on the side of the outlet chamber 31c near the inlet chamber 31b. This significantly improves the sealing performance between the outlet chamber 31c and the inlet chamber 31b, ensuring that the outlet chamber 31c and the inlet chamber 31b are not connected when the contact end 312 is separated from the solid-phase extraction column 40.

[0075] It should be noted that the type and setting method of the first seal 33 can be set according to the actual situation.

[0076] For example, the first seal 33 is a sealing ring, and a portion of the wall of the mounting channel 32b is recessed to form a mounting groove. The first seal 33 is engaged in the mounting groove and sleeved on the valve shaft 311.

[0077] In one embodiment, please refer to Figures 6 to 11 The positive pressure assembly 30 also includes a second seal 331, which is disposed at one end of the mounting channel 32b away from the intake chamber 31b and located on the side of the outlet chamber 31c away from the intake chamber 31b. This significantly improves the sealing performance between the outlet chamber 31c and the external environment, ensuring that gas in the outlet chamber 31c does not leak through the lower opening of the mounting channel 32b.

[0078] It should be noted that the type and setting method of the second seal 331 can be set according to the actual situation.

[0079] For example, the second seal 331 is a sealing ring, and a portion of the wall of the mounting channel 32b is recessed to form a mounting groove. The second seal 331 is engaged in the mounting groove and sleeved on the valve shaft 311.

[0080] In one embodiment, please refer to Figures 6 to 11 The positive pressure assembly 30 also includes a third seal 332. When the abutting end 312 abuts against the solid phase extraction column 40, the abutting end 312 blocks the opening of the solid phase extraction column 40. The third seal 332 is disposed at the junction of the abutting end 312 and the solid phase extraction column 40.

[0081] Specifically, the opening of the solid-phase extraction column 40 allows the abutment end 312 of the valve shaft 311 to extend into it, so that gas can enter the solid-phase extraction column 40 through the valve shaft 311. Therefore, the provision of a third seal 332 here can improve the sealing performance inside the solid-phase extraction column 40 and prevent gas from leaking from the connection between the valve shaft 311 and the solid-phase extraction column 40.

[0082] It should be noted that the type and setting method of the third seal 332 can be set according to the actual situation.

[0083] For example, the third seal 332 is a sealing ring, and a portion of the outer wall of the abutment end 312 is recessed to form a mounting groove, and the third seal 332 is engaged in the mounting groove.

[0084] In one embodiment, please refer to Figures 6 to 11 A portion of the outer surface of the contact end 312 protrudes to form a protrusion 3121, which is used to abut against the outer end face of the solid phase extraction column 40. The positive pressure assembly 30 also includes a first elastic member 34, which is sleeved on the valve shaft 311 and abuts against the protrusion 3121 and the positive pressure seat 32 at opposite ends.

[0085] Specifically, when the intake unit 31 abuts against the solid-phase extraction column 40, the protrusion 3121 abuts against the outer end face of the solid-phase extraction column 40, thereby restricting the valve shaft 311 from continuing to move towards the side closer to the solid-phase extraction column 40. As the positive pressure assembly 30 and the solid-phase extraction column 40 approach each other, the solid-phase extraction column 40 can push the valve shaft 311 upward in the opposite direction, thereby making the intake chamber 31b and the outlet chamber 31c interconnected.

[0086] Meanwhile, the protrusion 3121 can also be used for the first elastic member 34 to abut. By providing the first elastic member 34, on the one hand, during the process of the intake unit 31 switching from abutting with the solid phase extraction column 40 to separating from it, the valve shaft 311 can move away from the intake chamber 31b under the elastic force of the first elastic member 34, thereby resetting to the initial position that re-separates the intake chamber 31b and the outlet chamber 31c. On the other hand, when the abutting end 312 separates from the solid phase extraction column 40, an abutting force can always be applied to the valve shaft 311 to prevent the valve shaft 311 from loosening, thereby improving the sealing effect between the intake chamber 31b and the outlet chamber 31c.

[0087] In one embodiment, the positive pressure assembly 30 further includes a second elastic element disposed within the air intake chamber 31b, with its opposite ends abutting against the insertion end 313 and the positive pressure seat 32, respectively. By providing the second elastic element within the air intake chamber 31b, the valve shaft 311 can be easily reset. Furthermore, it ensures that an abutting force is always applied to the valve shaft 311 when the abutting end 312 separates from the solid-phase extraction column 40, preventing the valve shaft 311 from loosening. Simultaneously, it prevents the insertion end 313 from directly impacting or contacting the wall of the air intake chamber 31b.

[0088] In one embodiment, please refer to Figures 6 to 11The positive pressure assembly 30 also includes a baffle 35, a guide post 36, and a third elastic element 341. The guide post 36 is disposed on the side of the positive pressure seat 32 near the orifice plate 20. The baffle 35 is slidably sleeved on the guide post 36. The third elastic element 341 is sleeved on the guide post 36 and abuts against the baffle 35 and the positive pressure seat 32 respectively. The baffle 35 has multiple through holes 35a. The end of the valve shaft 311 facing away from the intake chamber 31b has an abutment end 312, which is inserted into the through hole 35a. The baffle 35 is used to abut against the outer end face of the solid phase extraction column 40. By setting the baffle 35, the solid phase extraction column 40 can be pressed during the process of switching the intake unit 31 from the connected state to the separated state, which can prevent the solid phase extraction column 40 from detaching from the orifice plate 20 along with the valve shaft 311.

[0089] Specifically, when the intake unit 31 is not in contact with the solid phase extraction column 40, the baffle 35 is restricted to the lower end of the guide column 36 by the third elastic element 341 and its own weight.

[0090] As the positive pressure assembly 30 moves toward the side closer to the solid-phase extraction column 40, the baffle 35 comes into contact with the solid-phase extraction column 40, and the solid-phase extraction column 40 pushes the baffle 35 toward the side of the positive pressure seat 32. The third elastic member 341 disposed between the baffle 35 and the positive pressure seat 32 is compressed, and the solid-phase extraction column 40 abuts against the valve shaft 311, so that the inlet chamber 31b and the outlet chamber 31c are connected.

[0091] When the gas supply process in the solid phase extraction column 40 ends, the positive pressure component 30 moves away from the solid phase extraction column 40. The baffle 35 presses the solid phase extraction column 40 under the action of the third elastic element 341, so that the solid phase extraction column 40 is pressed on the orifice plate 20. The valve shaft 311 gradually separates from the solid phase extraction column 40, while the solid phase extraction column 40 does not detach from the orifice plate 20.

[0092] It is understandable that the number of through holes 35a on the baffle 35 corresponds to the number of valve shafts 311, so that the valve shafts 311 can pass through and abut against the corresponding solid phase extraction column 40.

[0093] In one specific embodiment, please refer to Figures 6 to 8 When the intake unit 31 separates from the solid-phase extraction column 40, the lower end face of the contact end 312 is located inside the through hole 35a. This ensures that the baffle 35 remains pressed against the solid-phase extraction column 40 throughout the separation process between the valve shaft 311 and the solid-phase extraction column 40, resulting in a better pressing effect.

[0094] In one specific embodiment, please refer to Figures 6 to 11 The positive pressure component 30 can be raised and lowered relative to the base 10.

[0095] In the initial position, the positive pressure component 30 and the orifice plate 20 are spaced apart in the vertical direction. As the positive pressure component 30 gradually descends, the baffle 35 first comes into contact with the solid phase extraction column 40 on the orifice plate 20, and the third elastic element 341 is compressed.

[0096] As the positive pressure assembly 30 gradually descends, the valve shaft 311 comes into contact with the solid-phase extraction column 40. The solid-phase extraction column 40 pushes the valve shaft 311 relative to the positive pressure seat 32 towards the side closer to the inlet chamber 31b, thereby connecting the inlet chamber 31b and the outlet chamber 31c. When the positive pressure assembly 30 rises, the valve shaft 311, under the elastic action of the first elastic element 34, moves relative to the positive pressure seat 32 away from the inlet chamber 31b, thereby separating the inlet chamber 31b and the outlet chamber 31c again. As the positive pressure assembly 30 gradually rises, the valve shaft 311 separates from the solid-phase extraction column 40, which will not detach from the orifice plate 20 under the pressure of the baffle 35.

[0097] It is understandable that for the mounting hole 20a where the solid phase extraction column 40 is not installed, since there is no solid phase extraction column 40 to abut against the valve shaft 311 at the corresponding position, there will be no relative movement between the valve shaft 311 and the positive pressure seat 32. This allows the inlet chamber 31b and the outlet chamber 31c to always be separated, thus preventing gas leakage.

[0098] In one embodiment, please refer to Figure 2 , Figure 13 and Figure 14 The positive pressure column device also includes a temperature control component 50, which is vertically and flexibly mounted on the base 10. The temperature control component 50 has a downward-facing temperature control cavity 50a. The temperature control component 50 is raised and lowered to allow the orifice plate 20 to switch between entering and exiting the temperature control cavity 50a. This facilitates the installation of the orifice plate 20 into the temperature control component 50 and simplifies the structure of the temperature control component 50.

[0099] Specifically, the temperature control component 50 can be used to heat and control the temperature of the solid phase extraction column 40 mounted on the orifice plate 20. It can start heating according to the set parameters and keep the temperature within the range when the set temperature is reached.

[0100] An opening is formed at the bottom of the temperature control chamber 50a. By raising and lowering the temperature control component 50, the orifice plate 20 and the solid phase extraction column 40 on the orifice plate 20 can enter the temperature control chamber 50a.

[0101] It should be noted that the specific structure of the temperature control component 50 can be set according to the actual situation.

[0102] For example, please see Figure 13 and Figure 14The temperature control assembly 50 includes a silicone heating pad 51, a heat sink 52, and a temperature control base 53. The temperature control base 53 has a temperature control cavity 50a. The silicone heating pad 51 and the heat sink 52 are disposed within the temperature control cavity 50a, with the silicone heating pad 51 positioned on the side of the heat sink 52 facing away from the orifice plate 20. This allows for effective heating and temperature control of the solid-phase extraction column 40.

[0103] Specifically, the silicone heating pad 51 can convert electrical energy into heat energy. Depending on the actual situation, a temperature sensor can also be installed inside the silicone heating pad 51 to provide real-time temperature feedback. Based on the real-time temperature and the preset temperature, the heating power is adjusted until the preset temperature is reached and maintained.

[0104] The heat sink 52 has good heat transfer performance, enabling a more uniform distribution of heat within the temperature control cavity 50a.

[0105] In one embodiment, please refer to Figure 1 and Figure 2 The positive pressure column extraction device includes a lifting assembly 60 and a translation assembly 70. The lifting assembly 60 is mounted on the base 10. The temperature control assembly 50 and the positive pressure assembly 30 are mounted on the lifting assembly 60 along a first direction, allowing the lifting assembly 60 to move up and down relative to the base 10. The translation assembly 70 is slidably mounted on the base 10 along the first direction, and the orifice plate 20 is mounted on the translation assembly 70, allowing the translation assembly 70 to move relative to the base 10. Thus, through the translation function of the translation assembly 70 and the lifting function of the lifting assembly 60, the solid-phase extraction column 40 can be effectively matched with the temperature control assembly 50 and the positive pressure assembly 30, respectively.

[0106] Specifically, the orifice plate 20 and the solid phase extraction column 40 can be moved along the first direction by the translation component 70 to the lower side of the positive pressure component 30. Under the drive of the lifting component 60, the positive pressure component 30 can perform positive pressure column passage operation on the solid phase extraction column 40 by lifting and lowering.

[0107] When it is necessary to heat and control the solid phase extraction column 40 through the temperature control component 50, the orifice plate 20 and the solid phase extraction column 40 can be moved along the first direction to the lower side of the temperature control component 50 through the translation component 70, and the temperature control component 50 can be raised and lowered through the lifting component 60 to heat and control the solid phase extraction column 40.

[0108] In one specific embodiment, the positive pressure column device further includes a first positioning structure and a second positioning structure. The first positioning structure is disposed on the base 10 and located on the bottom side of the positive pressure component 30. The first positioning structure is used to position and cooperate with the translation component 70 so that the translation component 70 can be better positioned on the bottom side of the positive pressure component 30 during movement along the first direction. The second positioning structure is disposed on the base 10 and located on the bottom side of the temperature control component 50. The second positioning structure is used to position and cooperate with the translation component 70 so that the translation component 70 can be better positioned on the bottom side of the temperature control component 50 during movement along the first direction.

[0109] It should be noted that the specific structure of the first and second positioning structures is not limited. For example, a snap-fit ​​structure or a magnetic structure can be used.

[0110] It should be noted that the specific structure of the lifting component 60 can be set according to the actual situation.

[0111] In one embodiment, please refer to Figure 1 and Figure 3 The positive pressure column-passing device includes a lifting assembly 60, which comprises an upper support plate 61, a lifting support plate 62, a synchronous pulley structure 63, a drive motor 64, a ball screw nut 65, and a ball screw 66. The ball screw 66 is rotatably mounted on the base 10. The upper support plate 61 is sleeved on the ball screw 66, and the lifting support plate 62 is sleeved on the ball screw 66 via the ball screw nut 65. The positive pressure assembly 30 is mounted on the lifting support plate 62, and the drive motor 64 and the synchronous pulley structure 63 are mounted on the upper support plate 61. The drive motor 64 is connected to the ball screw 66 via the synchronous pulley structure 63, so that by driving the ball screw 66 to rotate, the lifting support plate 62 drives the positive pressure assembly 30 to rise and fall. Therefore, by activating the drive motor 64, the overall lifting and falling of the positive pressure assembly 30 can be effectively achieved, thus improving the stability of the overall lifting and falling of the positive pressure assembly 30.

[0112] Specifically, the upper support plate 61 is used to fix and install the drive motor 64 and the synchronous pulley structure 63, while the lifting support plate 62 is used to install the positive pressure assembly 30. The ball screw 66 passes through the upper support plate 61, the lifting support plate 62, and the base 10 in sequence, and can rotate relative to the three. The lifting support plate 62 and the ball screw 66 are connected by a ball screw nut 65. Thus, through the cooperation of the ball screw 66 and the ball screw nut 65, rotation can be converted into linear motion, allowing the ball screw nut 65 and the lifting support plate 62 to move along the axial direction of the ball screw 66, thereby driving the positive pressure assembly 30 to rise and fall.

[0113] Depending on the actual situation, the temperature control component 50 can also be set on the lifting support plate 62, so that the lifting support plate 62 can simultaneously drive the temperature control component 50 and the positive pressure component 30 to rise and fall.

[0114] It should be noted that the specific number of ball screws 66 can be set according to the actual situation.

[0115] For example, the lifting assembly 60 includes two ball screws 66 and two rotating shafts, which are also mounted on the upper support plate 61, the lifting support plate 62 and the base 10.

[0116] The specific structure of the synchronous belt pulley structure 63 can be set according to the actual situation.

[0117] For example, please refer to Figure 1 and Figure 3 The synchronous pulley structure 63 includes multiple synchronous pulleys 631 and a synchronous belt 632. The synchronous pulleys 631 are rotatably mounted on the upper support plate 61. The drive motor 64 is driven by one of the synchronous pulleys 631. A synchronous pulley 631 is provided at the end of the ball screw 66. Each synchronous pulley 631 is connected by a synchronous belt 632.

[0118] In some embodiments, please refer to Figure 1 and Figure 3 The synchronous belt pulley structure 63 also includes a tensioner 633 and an idler 634, which are respectively connected to the synchronous belt 632 for transmission.

[0119] The specific structure of the translation component 70 can be set according to the actual situation.

[0120] For example, please see Figure 2 The translation assembly 70 includes a linear guide rail 71, a lead screw motor 72, a lead screw nut 73, a slide adapter 74, a bearing seat 75, and a motor seat 76. The bearing seat 75 and motor seat 76 are spaced apart on the base 10 along a first direction. The lead screw motor 72 is mounted on the motor seat 76, and its lead screw extends along the first direction to the bearing seat 75 and is rotatable relative to the bearing seat 75 and motor seat 76. The linear guide rail 71 is located below the lead screw. The perforated plate 20 is mounted on the slide adapter 74, which is slidably mounted on the linear guide rail 71 and connected to the lead screw of the lead screw motor 72 via the lead screw nut 73. Thus, by driving the lead screw nut 73 to move via the lead screw motor 72, the slide adapter 74 and the perforated plate 20 can be moved along the first direction.

[0121] In one specific embodiment, the translation component 70 includes a slide adapter 74. When the orifice plate 20 is located inside the temperature control cavity 50a, the slide adapter 74 closes the lower opening of the temperature control cavity 50a. The slide adapter 74 has a groove formed around the orifice plate 20, and the wall at the lower opening of the temperature control cavity 50a is engaged in the groove, thereby improving the sealing and heat preservation performance of the temperature control cavity 50a.

[0122] In the description of this application, the references to terms such as "in one embodiment," "in some embodiments," "in a specific embodiment," or "exemplary," etc., refer to a specific feature, structure, material, or characteristic described in connection with that embodiment or example, which is included in at least one embodiment or example of the embodiments of this application. In this application, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Furthermore, without contradiction, those skilled in the art can combine the different embodiments or examples described in this application, as well as the features of the different embodiments or examples.

[0123] The above description is merely a preferred embodiment of this application and is not intended to limit the application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application are included within the scope of protection of this application.

Claims

1. A positive pressure column conveying device, characterized in that, Includes the base, orifice plate, and positive pressure assembly; The perforated plate is disposed on the base and has multiple mounting holes for accommodating a solid phase extraction column. The positive pressure component is disposed on the base and spaced apart from the base to form a positive pressure space, the positive pressure space being used to accommodate the orifice plate; the positive pressure component has an air inlet and multiple air inlet units, each air inlet unit including an air outlet, an air inlet chamber and an air outlet chamber, the air inlet chamber communicating with the air inlet, the air outlet chamber communicating with the air outlet, and the air outlet corresponding to the mounting hole for supplying gas into the solid phase extraction column; The air intake unit has a connected state and a separated state; the air intake unit connects with the solid phase extraction column to form an airflow path that sequentially passes through the air inlet, the air intake chamber, the air outlet chamber, the air outlet, and the solid phase extraction column, and the air intake unit is in the connected state; when the air intake unit separates from the solid phase extraction column, the air intake chamber and the air outlet chamber are separated to block the airflow path, and the air intake unit is in the separated state.

2. The positive pressure column conveying device according to claim 1, characterized in that, The positive pressure assembly includes a positive pressure seat with multiple installation spaces. A portion of the installation space forms the air intake chamber. The air intake unit includes a valve shaft, which is movably disposed in the installation space to form the air outlet chamber with the positive pressure seat within the installation space. The valve shaft switches between the connected state and the separated state by contacting and separating from the solid phase extraction column.

3. The positive pressure column conveying device according to claim 2, characterized in that, The valve shaft has an abutment end at one end away from the air inlet chamber. The abutment end extends out of the installation space for contact with the solid phase extraction column. The valve shaft has a flow channel extending in the extension direction. The abutment end has the air outlet. The opposite ends of the flow channel are respectively connected to the air outlet chamber and the air outlet.

4. The positive pressure column conveying device according to claim 2, characterized in that, The valve shaft has an insertion end, a valve stem section, and an abutment end. The valve stem section is located between the insertion end and the abutment end. Another part of the mounting space forms a mounting channel. The valve shaft is movably inserted into the mounting channel. A portion of the outer wall of the valve stem section is recessed to form the air outlet chamber by being spaced apart from the wall of the mounting channel. The insertion end extends into the air inlet chamber.

5. The positive pressure column conveying device according to claim 4, characterized in that, When the abutting end separates from the solid-phase extraction column, at least one of the extending end and the valve stem section blocks the installation channel to separate the air inlet chamber and the air outlet chamber; when the abutting end abuts against the solid-phase extraction column, the valve shaft moves toward the side closer to the air inlet chamber to connect the air inlet chamber and the air outlet chamber.

6. The positive pressure column conveying device according to claim 4, characterized in that, The cross-sectional dimension of the air intake chamber is larger than that of the installation channel to form a first limiting step at the connection point; the cross-sectional dimension of the extended end is larger than that of the valve stem section to form a second limiting step at the connection point; when the air intake unit is in the separated state, the first limiting step and the second limiting step are in contact with each other; when the air intake unit is in the connected state, the first limiting step and the second limiting step are separated from each other.

7. The positive pressure column conveying device according to claim 4, characterized in that, The positive pressure assembly further includes a first seal, which is disposed at one end of the mounting channel near the air inlet chamber and located on the side of the air outlet chamber near the air inlet chamber; and / or, The positive pressure assembly further includes a second seal, which is disposed at one end of the mounting channel opposite to the air inlet chamber and located on the side of the air outlet chamber opposite to the air inlet chamber; and / or, The positive pressure assembly also includes a third seal, which blocks the opening of the solid phase extraction column when the abutting end abuts against the solid phase extraction column, and the third seal is disposed at the junction of the abutting end and the solid phase extraction column.

8. The positive pressure column conveying device according to claim 4, characterized in that, A portion of the outer surface of the abutting end protrudes to form a raised portion, which abuts against the outer end face of the solid-phase extraction column; the positive pressure assembly further includes a first elastic element, which is sleeved on the valve shaft and abuts against the raised portion and the positive pressure seat at opposite ends; and / or, The positive pressure assembly further includes a second elastic element, which is disposed in the air intake chamber and its opposite ends abut against the extension end and the positive pressure seat, respectively.

9. The positive pressure column conveying device according to claim 2, characterized in that, The positive pressure assembly further includes a baffle, a guide post, and a third elastic element. The guide post is disposed on the side of the positive pressure seat near the orifice plate. The baffle is slidably sleeved on the guide post. The third elastic element is sleeved on the guide post and abuts against the baffle and the positive pressure seat respectively. The baffle has multiple through holes. The end of the valve shaft opposite to the air inlet chamber has an abutting end. The abutting ends are correspondingly inserted into the through holes. The baffle is used to abut against the outer end face of the solid phase extraction column.

10. The positive pressure column conveying device according to claim 9, characterized in that, When the air intake unit separates from the solid phase extraction column, the lower end face of the contact end is located inside the through hole.

11. The positive pressure column conveying device according to any one of claims 1-10, characterized in that, The positive pressure column device also includes a temperature control component, which is vertically and flexibly mounted on the base. The temperature control component has a downward-facing temperature control cavity, and the temperature control component switches the orifice plate between entering and exiting the temperature control cavity by raising and lowering itself.

12. The positive pressure column conveying device according to claim 11, characterized in that, The temperature control component includes a silicone heating pad, a heat sink, and a temperature control base. The temperature control base has a temperature control cavity. The silicone heating pad and the heat sink are disposed in the temperature control cavity, and the silicone heating pad is disposed on the side of the heat sink away from the perforated plate.

13. The positive pressure column conveying device according to claim 11, characterized in that, The positive pressure column conveyor includes a lifting assembly and a translation assembly. The lifting assembly is disposed on the base body. The temperature control assembly and the positive pressure assembly are disposed on the lifting assembly along a first direction so that the lifting assembly can move up and down relative to the base body in the vertical direction. The translation assembly is slidably disposed on the base body along the first direction. The perforated plate is disposed on the translation assembly so that the translation assembly can move relative to the base body.

14. The positive pressure column conveying device according to any one of claims 1-10, characterized in that, The positive pressure column-passing device includes a lifting assembly, which comprises an upper support plate, a lifting support plate, a synchronous pulley structure, a drive motor, a ball screw nut, and a ball screw. The ball screw is rotatably mounted on the base body. The upper support plate is sleeved on the ball screw. The lifting support plate is sleeved on the ball screw via the ball screw nut. The positive pressure assembly is mounted on the lifting support plate. The drive motor and the synchronous pulley structure are mounted on the upper support plate. The drive motor is connected to the ball screw via the synchronous pulley structure, so that by driving the ball screw to rotate, the lifting support plate drives the positive pressure assembly to rise and fall.

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