A positive pressure over column apparatus

Abstract

The embodiment of the present application provides a positive pressure column passing device, which comprises a seat body, a hole plate and a positive pressure assembly. The positive pressure assembly has a gas inlet and a plurality of gas inlet units. The gas inlet unit comprises a gas outlet, a gas inlet cavity and a gas outlet cavity. The gas inlet cavity is communicated with the gas inlet, and the gas outlet cavity is communicated with the gas outlet. The gas outlet corresponds to the mounting hole, so as to be used for the gas to enter the solid phase extraction column. The gas inlet unit has a communication state and a separation state. The gas inlet unit is abutted with the solid phase extraction column, so that the gas inlet cavity and the gas outlet cavity are communicated, and a gas flow path passing through the gas inlet, the gas inlet cavity, the gas outlet cavity, the gas outlet and the solid phase extraction column in sequence is formed. The gas inlet unit is in the communication state. When the gas inlet unit is separated from the solid phase extraction column, the gas inlet cavity and the gas outlet cavity are separated to cut off the gas flow path, and the gas inlet unit is in the separation state. The positive pressure column passing device of the embodiment of the present application can reduce the risk of gas leakage.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of positive pressure column passing, and particularly relates to a positive pressure column passing device. BACKGROUND

[0002] In the related art, the positive pressure column passing device realizes sealing by overall force deformation of the end face of the compressible material, and then gas enters. When there is a missing solid phase extraction (SPE) column on the hole plate (for example, there are only 95 SPE columns in a 96-hole plate), the positive pressure column passing device is not completely sealed locally, which easily causes gas leakage, thereby causing insufficient extraction of the SPE column within the set time pressure. SUMMARY

[0003] Therefore, the main purpose of the embodiments of the present application is to provide a positive pressure column passing device capable of reducing the risk of gas leakage.

[0004] To achieve the above purpose, the technical scheme of the embodiments of the present application is as follows:

[0005] The positive pressure column passing device provided by the embodiments of the present application comprises a seat body, a hole plate and a positive pressure assembly.

[0006] The hole plate is arranged on the seat body and has a plurality of mounting holes for accommodating solid phase extraction columns.

[0007] The positive pressure assembly is arranged on the seat body and spaced from the seat body to form a positive pressure space for accommodating the hole plate. The positive pressure assembly has a gas inlet and a plurality of gas inlet units. The gas inlet unit comprises a gas outlet, a gas inlet cavity and a gas outlet cavity. The gas inlet cavity is in communication with the gas inlet, and the gas outlet cavity is in communication with the gas outlet. The gas outlet corresponds to the mounting hole, so as to allow gas to enter the solid phase extraction column.

[0008] The gas inlet unit has a communication state and a separation state. The gas inlet unit is in abutment with the solid phase extraction column corresponding to the gas inlet unit, so as to communicate between the gas inlet cavity and the gas outlet cavity, and form a gas flow path passing through the gas inlet, the gas inlet cavity, the gas outlet cavity, the gas outlet and the solid phase extraction column in sequence. The gas inlet unit is in the communication state. When the gas inlet unit is separated from the solid phase extraction column, the gas inlet cavity and the gas outlet cavity are arranged in a separated manner to cut off the gas flow path, and the gas inlet unit is in the separation state.

[0009] In one embodiment, the positive pressure assembly comprises a positive pressure seat having a plurality of mounting spaces, a portion of the mounting spaces forming the air inlet cavity, and the air inlet unit comprises a valve shaft movably arranged in the mounting spaces one by one to form the air outlet cavity with the positive pressure seat in the mounting spaces, the valve shaft being abutted and separated from the solid phase extraction column to switch the air inlet unit between the communication state and the separation state.

[0010] In one embodiment, the valve shaft has an abutment end at one end away from the air inlet cavity, the abutment end extending out of the mounting space for abutting the solid phase extraction column, the valve shaft having a flow passage extending in the extending direction inside the valve shaft, and the abutment end having the air outlet port, the opposite ends of the flow passage being in communication with the air outlet cavity and the air outlet port respectively.

[0011] In one embodiment, the valve shaft has a protruding end, a valve stem segment, and an abutment end, the valve stem segment being located between the protruding end and the abutment end, another portion of the mounting spaces forming a mounting passage, the valve shaft movably arranged in the mounting passage, and a portion of the outer side wall of the valve stem segment being recessed to form the air outlet cavity with the wall of the mounting passage spaced, the protruding end protruding into the air inlet cavity.

[0012] In one embodiment, when the abutment end is separated from the solid phase extraction column, at least one of the protruding end and the valve stem segment blocks the mounting passage to separate the air inlet cavity and the air outlet cavity; when the abutment end is abutted with the solid phase extraction column, the valve shaft moves towards the side close to the air inlet cavity to communicate the air inlet cavity and the air outlet cavity.

[0013] In one embodiment, the cross-sectional dimension of the air inlet cavity is greater than the cross-sectional dimension of the mounting passage to form a first limiting step at the communication; the cross-sectional dimension of the protruding end is greater than the cross-sectional dimension of the valve stem segment to form a second limiting step at the connection; when the air inlet unit is in the separation state, the first limiting step and the second limiting step correspondingly fit; when the air inlet unit is in the communication state, the first limiting step and the second limiting step are separated from each other.

[0014] In one embodiment, the positive pressure assembly further comprises a first sealing member arranged at one end of the mounting passage close to the air inlet cavity and located at one side of the air outlet cavity close to the air inlet cavity.

[0015] In one embodiment, the positive pressure assembly further comprises a second sealing member arranged at one end of the mounting passage away from the air inlet cavity and located at one side of the air outlet cavity away from the air inlet cavity.

[0016] In one embodiment, the positive pressure assembly further comprises a third sealing member, when the abutting end abuts against the solid phase extraction column, the abutting end blocks the opening of the solid phase extraction column, and the third sealing member is arranged at the abutting end and the solid phase extraction column.

[0017] In one embodiment, a part of the outer surface of the abutting end is protruded to form a protruding part, and the protruding part is used to abut against the outer end surface of the solid phase extraction column; the positive pressure assembly further comprises a first elastic member, the first elastic member is sleeved on the valve shaft, and the opposite ends of the first elastic member abut against the protruding part and the positive pressure seat respectively.

[0018] In one embodiment, the positive pressure assembly further comprises a second elastic member, the second elastic member is arranged in the air inlet cavity, and the opposite ends of the second elastic member abut against the protruding end and the positive pressure seat respectively.

[0019] In one embodiment, the positive pressure assembly further comprises a baffle, a guide column and a third elastic member, the guide column is arranged on one side of the positive pressure seat close to the hole plate, the baffle is slidably sleeved on the guide column, the third elastic member is sleeved on the guide column, and the baffle and the positive pressure seat abut against each other, the baffle has a plurality of through holes, one end of the valve shaft away from the air inlet cavity has an abutting end, the abutting end is correspondingly arranged in the through hole, and the baffle is used to abut against the outer end surface of the solid phase extraction column.

[0020] In one embodiment, the lower end surface of the abutting end is located in the through hole.

[0021] In one embodiment, the positive pressure column equipment further comprises a temperature control assembly, the temperature control assembly is arranged on the seat body in a lifting manner, the temperature control assembly has a temperature control cavity with an opening downward, and the temperature control assembly is lifted to switch the hole plate between entering and leaving the temperature control cavity.

[0022] In one embodiment, the temperature control assembly comprises a silica gel heating pad, a heat dissipation plate and a temperature control seat, the temperature control seat has the temperature control cavity, the silica gel heating pad and the heat dissipation plate are arranged in the temperature control cavity, and the silica gel heating pad is arranged on the side of the heat dissipation plate away from the hole plate.

[0023] In one embodiment, the positive pressure column equipment comprises a lifting assembly and a translation assembly, the lifting assembly is arranged on the seat body, the temperature control assembly and the positive pressure assembly are arranged on the lifting assembly in a first direction, so as to be lifted up and down relative to the seat body through the lifting assembly, and the translation assembly is slidably arranged on the seat body in the first direction, and the hole plate is arranged on the translation assembly, so as to move relative to the seat body through the translation assembly.

[0024] In an embodiment, the positive pressure column passing device comprises a lifting assembly, the lifting assembly comprises an upper support plate, a lifting support plate, a synchronous belt wheel structure, a driving motor, a ball screw nut and a ball screw, the ball screw is rotatably arranged on the seat body, the upper support plate is sleeved on the ball screw, the lifting support plate is sleeved on the ball screw through the ball screw nut, the positive pressure assembly is arranged on the lifting support plate, the driving motor and the synchronous belt wheel structure are arranged on the upper support plate, the driving motor is drivingly connected with the ball screw through the synchronous belt wheel structure, so that the lifting support plate drives the positive pressure assembly to lift by driving the ball screw to rotate.

[0025] The positive pressure column passing device provided by the embodiment of the application comprises a seat body, a hole plate and a positive pressure assembly. An air inlet cavity of the positive pressure assembly is in communication with an air inlet, and an air outlet cavity is in communication with an air outlet. An air inlet unit has a communication state and a separation state. The air inlet unit is in abutment with the solid-phase extraction column to enable the air inlet cavity and the air outlet cavity to be in communication, and to form an air flow path that sequentially passes through the air inlet, the air inlet cavity, the air outlet cavity, the air outlet and the solid-phase extraction column. The air inlet unit is in the communication state. When the air inlet unit is separated from the solid-phase extraction column, the air inlet cavity and the air outlet cavity are separated to cut off the air flow path, and the air inlet unit is in the separation state. That is, the air inlet unit of the application can correspondingly realize the separation and communication of the air inlet cavity and the air outlet cavity by switching between separation and abutment with the solid-phase extraction column. Thus, when the hole plate is missing the solid-phase extraction column (i.e., there is a case where the solid-phase extraction column is not installed in some of the mounting holes), only the air inlet unit in abutment with the solid-phase extraction column is in the communication state and can normally supply air to the solid-phase extraction column. The air inlet unit that is not in abutment with the solid-phase extraction column (i.e., is separated from the solid-phase extraction column) is in the separation state, and the air inlet cavity and the air outlet cavity of the air inlet unit are not in communication. Thus, the gas of the positive pressure column passing device cannot leak from the air inlet unit, thereby reducing the risk of gas leakage and enabling the solid-phase extraction column to be more fully extracted within a set time pressure. Meanwhile, the operator can flexibly arrange the number of solid-phase extraction columns on the hole plate, and the number of solid-phase extraction columns in a single test process does not need to strictly correspond to the mounting holes, thereby improving the operation convenience of the positive pressure column passing device. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 FIG. 1 is a structural schematic diagram of a positive pressure column passing device according to an embodiment of the application;

[0027] Figure 2 FIG. 2 is a structural schematic diagram of a positive pressure column passing device according to another embodiment of the application; Figure 1 FIG. 3 is a sectional view of the positive pressure column passing device along a first direction in FIG. 2;

[0028] Figure 3 FIG. 4 is a sectional view of the positive pressure column passing device along a second direction in FIG. 2; and Figure 1Structure diagram of the pressure over column device from another perspective;

[0029] Figure 4 For Figure 1 Structure diagram of the cooperation between the middle hole plate and the solid phase extraction column;

[0030] Figure 5 For Figure 2 A-A direction sectional view;

[0031] Figure 6 For Figure 5 Local enlarged view at B;

[0032] Figure 7 For Figure 6 Structure diagram of the cooperation between the air inlet unit, the baffle and the solid phase extraction column; in the diagram, the air inlet unit and the solid phase extraction column are separated, and the baffle abuts against the solid phase extraction column;

[0033] Figure 8 For Figure 6 Structure diagram of the cooperation between the air inlet unit, the baffle and the solid phase extraction column; in the diagram, the air inlet unit is separated from the solid phase extraction column, and the baffle abuts against the solid phase extraction column;

[0034] Figure 9 For Figure 6 Structure diagram of the cooperation between the air inlet unit, the baffle and the solid phase extraction column; in the diagram, the air inlet unit abuts against the solid phase extraction column, the baffle abuts against the solid phase extraction column, and the air inlet cavity is separated from the air outlet cavity;

[0035] Figure 10 For Figure 6 Structure diagram of the cooperation between the air inlet unit, the baffle and the solid phase extraction column; in the diagram, the air inlet unit abuts against the solid phase extraction column, the baffle abuts against the solid phase extraction column, and the air inlet cavity is communicated with the air outlet cavity;

[0036] Figure 11 For Figure 6 Structure diagram of the air inlet unit not abutting against the solid phase extraction column;

[0037] Figure 12 For Figure 6 Partial structure diagram of the pressure seat;

[0038] Figure 13 For Figure 2 C-C direction sectional view;

[0039] Figure 14 For Figure 13 Structure diagram of the cooperation between the hole plate, the solid phase extraction column and the temperature control assembly; in the diagram, the hole plate and the solid phase extraction column are located in the temperature control cavity.

[0040] Explanation of reference signs

[0041] 10, seat body; 20, aperture plate; 20a, mounting hole; 30, positive pressure assembly; 30a, positive pressure space; 30b, air inlet; 31, air inlet unit; 31a, air outlet; 31b, air inlet cavity; 31c, air outlet cavity; 311, valve shaft; 311a, flow passage; 312, abutting end; 3121, protruding part; 313, extending end; 314, valve stem section; 315, second limiting step; 32, positive pressure seat; 32a, mounting space; 32b, mounting channel; 321, first limiting step; 33, first sealing member; 331, second sealing member; 332, third sealing member; 34, first elastic member; 341, third elastic member; 35, baffle; 35a, via hole; 36, guide column; 40, solid phase extraction column; 50, temperature control assembly; 50a, temperature control cavity; 51, silica gel heating pad; 52, heat sink; 53, temperature control seat; 60, lifting assembly; 61, upper support plate; 62, lifting support plate; 63, synchronous belt wheel structure; 631, synchronous belt wheel; 632, synchronous belt; 633, tension pulley; 634, idler; 64, drive motor; 65, ball screw nut; 66, ball screw; 70, translation assembly; 71, linear guide rail; 72, screw motor; 73, screw nut; 74, sliding table adapter; 75, bearing seat; 76, motor seat. DETAILED DESCRIPTION

[0042] In the present application, the "up-down direction", "first direction" orientation or positional relationship is based on the orientation or positional relationship shown in the drawings. Figure 2 It should be understood that these orientation terms are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0043] In the description of the embodiments of the present application, the technical terms "first", "second", "third" and the like are only used to distinguish different objects, and cannot be understood as indicating or implying relative importance or implicitly indicating the number, specific order or primary and secondary relationship of the technical features indicated. In the description of the embodiments of the present application, the meaning of "multiple" is two and more than two, unless otherwise explicitly and specifically limited.

[0044] Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The occurrence of the phrase in various places in the specification is not necessarily all a reference to the same embodiment, or is necessarily an independent or alternative embodiment to other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined.

[0045] An embodiment of the present application provides a positive pressure column passing device, please refer to Figures 1 to 3The positive pressure column device comprises a seat body 10, a hole plate 20 and a positive pressure assembly 30.

[0046] Referring to Figure 4 The hole plate 20 is arranged on the seat body 10 and has a plurality of mounting holes 20a for accommodating the solid phase extraction column 40.

[0047] The positive pressure assembly 30 is arranged on the seat body 10 and spaced from the seat body 10 to form a positive pressure space 30a for accommodating the hole plate 20; the positive pressure assembly 30 has a gas inlet 30b and a plurality of gas inlet units 31, each of which comprises a gas outlet 31a, a gas inlet cavity 31b and a gas outlet cavity 31c, the gas inlet cavity 31b is in communication with the gas inlet 30b, the gas outlet cavity 31c is in communication with the gas outlet 31a, and the gas outlet 31a corresponds to the mounting hole 20a for the gas to enter the solid phase extraction column 40.

[0048] Referring to Figures 5 to 11 The gas inlet unit 31 has a communication state and a separation state; the gas inlet unit 31 is abutted corresponding to the solid phase extraction column 40 to make the gas inlet cavity 31b and the gas outlet cavity 31c in communication, and form a gas flow path passing through the gas inlet 30b, the gas inlet cavity 31b, the gas outlet cavity 31c, the gas outlet 31a and the solid phase extraction column 40 in sequence, and the gas inlet unit 31 is in the communication state; when the gas inlet unit 31 is separated from the solid phase extraction column 40, the gas inlet cavity 31b and the gas outlet cavity 31c are arranged in separation to cut off the gas flow path, and the gas inlet unit 31 is in the separation state.

[0049] Specifically, the positive pressure column device can be used for processing various samples. For example, the positive pressure column device is used for pretreating a biological sample before mass spectrometric analysis. By pretreating the biological sample, including protein extraction, protein reduction, alkylation, enzymatic hydrolysis, and removing possible interferents in the protein sample, such as salts, lipids, nucleic acids, etc., the protein can be purified or the clean polypeptide after enzymatic hydrolysis can be obtained, so as to improve the signal-to-noise ratio, the number of protein identifications and the quantitative accuracy of mass spectrometric analysis. Moreover, high-precision temperature control is required during the pretreatment process to ensure the stable and smooth progress of biochemical reactions such as protein reduction, alkylation and enzymatic hydrolysis. The present application describes the positive pressure column device for pretreating a protein sample as an example.

[0050] The solid phase extraction column 40 is mounted on the mounting hole 20a of the hole plate 20 and is used for accommodating the protein sample.

[0051] It should be noted that the hole plate 20 has a plurality of mounting holes 20a, the number of which is not limited, such as 96 mounting holes 20a. Each mounting hole 20a can correspondingly install a solid phase extraction column 40. According to actual needs, some of the mounting holes 20a can correspondingly install a solid phase extraction column 40, and the other mounting holes 20a can not install a solid phase extraction column 40. In this way, the operator can flexibly arrange the solid phase extraction column 40, and it is not required that the number of solid phase extraction columns 40 in a single experiment must correspond to the number of mounting holes 20a, and the installation position of the solid phase extraction column 40 can be randomly set. For example, the solid phase extraction column 40 can be arranged at intervals on the hole plate 20.

[0052] The positive pressure assembly 30 is used for positive pressure column passing of the solid phase extraction column 40. The protein sample is pretreated by using the positive pressure column passing mode, which can facilitate the compatibility of the positive pressure column passing equipment in the automatic pipetting workstation and can be flexibly configured. Compared with the centrifugal column passing mode, a high-integration-degree device is not required, and the volume is relatively small.

[0053] The space between the positive pressure assembly 30 and the seat body 10 is a positive pressure space 30a, which is used for the hole plate 20 to enter and perform the positive pressure column passing operation.

[0054] The gas inlet 30b of the positive pressure assembly 30 is used for the gas to enter.

[0055] The positive pressure assembly 30 has a plurality of gas inlet units 31, and one gas inlet unit 31 is used to correspond to the solid phase extraction column 40 on one mounting hole 20a. Of course, according to actual needs, each gas inlet unit 31 can correspond to one solid phase extraction column 40. When some of the mounting holes 20a are not installed with the solid phase extraction column 40, only some of the gas inlet units 31 can correspond to one solid phase extraction column 40, and the other gas inlet units 31 can not correspond to the solid phase extraction column 40.

[0056] It should be noted that the gas inlet unit 31 is a unit in the positive pressure assembly 30, each of which is used for the gas from the gas inlet 30b to enter the solid phase extraction column 40.

[0057] Each gas inlet unit 31 includes a gas outlet 31a, a gas inlet cavity 31b in communication with the gas inlet 30b, and a gas outlet cavity 31c in communication with the gas outlet 31a. Among them, the gas outlet 31a corresponds to the position of the mounting hole 20a one by one, when the corresponding position of the mounting hole 20a is installed with the solid phase extraction column 40, the gas outlet 31a is in communication with the opening of the solid phase extraction column 40, so that the gas enters the solid phase extraction column 40.

[0058] It should be noted that the gas inlet cavity 31b and the gas outlet cavity 31c have two states of communication and separation, and the switching of the above two states is determined by whether the gas inlet unit 31 is in abutment with the solid phase extraction column 40.

[0059] Specifically, when the solid phase extraction column 40 is installed on the mounting hole 20a, the gas inlet unit 31 corresponding to the position of the mounting hole 20a is in abutment with the solid phase extraction column 40, so that the gas inlet cavity 31b and the gas outlet cavity 31c of the gas inlet unit 31 are in communication. Thus, the gas inlet 30b, the gas inlet cavity 31b, the gas outlet cavity 31c and the gas outlet 31a are sequentially communicated, the gas flowing from the gas inlet 30b can sequentially flow along the above-mentioned cavities, and then enter the solid phase extraction column 40, thereby forming a gas flow path sequentially passing through the gas inlet 30b, the gas inlet cavity 31b, the gas outlet cavity 31c, the gas outlet 31a and the solid phase extraction column 40.

[0060] When the solid phase extraction column 40 is not installed on the mounting hole 20a, or the solid phase extraction column 40 is installed on the mounting hole 20a but is not in abutment with the corresponding gas inlet unit 31, the gas inlet unit 31 is separated from the solid phase extraction column 40, so that the gas inlet cavity 31b and the gas outlet cavity 31c of the gas inlet unit 31 are separated, and they are not in communication. The gas cannot flow from the gas inlet 30b and the gas inlet cavity 31b to the gas outlet cavity 31c, so there is no gas leakage, and the above-mentioned gas flow path is cut off.

[0061] In the positive pressure column passing device in the embodiments of the present application, the air inlet cavity 31b of the positive pressure assembly 30 is in communication with the air inlet 30b, and the air outlet cavity 31c is in communication with the air outlet 31a. The air inlet unit 31 has a communication state and a separation state; the air inlet unit 31 is in abutment with the solid phase extraction column 40 to make the air inlet cavity 31b and the air outlet cavity 31c in communication, and a gas flow path is formed in sequence through the air inlet 30b, the air inlet cavity 31b, the air outlet cavity 31c, the air outlet 31a, and the solid phase extraction column 40, and the air inlet unit 31 is in the communication state. When the air inlet unit 31 is separated from the solid phase extraction column 40, the air inlet cavity 31b and the air outlet cavity 31c are separated to cut off the gas flow path, and the air inlet unit 31 is in the separation state. That is, the air inlet unit 31 of the present application can realize the separation and communication of the air inlet cavity 31b and the air outlet cavity 31c by switching between separation and abutment with the solid phase extraction column 40. Thus, when the hole plate 20 is missing the solid phase extraction column 40 (i.e., there is a case where the solid phase extraction column 40 is not installed on part of the mounting hole 20a), only the air inlet unit 31 in abutment with the solid phase extraction column 40 is in the communication state, and can normally supply gas to the solid phase extraction column 40. The air inlet unit 31 not in abutment with the solid phase extraction column 40 (i.e., separated from the solid phase extraction column 40) is in the separation state, and the air inlet cavity 31b and the air outlet cavity 31c of the air inlet unit 31 are not in communication. Thus, the gas of the positive pressure column passing device cannot leak from the air inlet unit 31, thereby reducing the risk of gas leakage and enabling the solid phase extraction column 40 to be more fully extracted within a set time pressure. Moreover, it can also prevent the case in the related art where the force on part of the solid phase extraction column 40 is inconsistent, causing gas leakage. At the same time, it can facilitate the operator to flexibly arrange the number of solid phase extraction columns 40 on the hole plate 20, and the number of solid phase extraction columns 40 in a single test process does not need to strictly correspond to the mounting hole 20a, thereby improving the operation convenience of the positive pressure column passing device.

[0062] In an embodiment, referring to Figures 6 to 11 , the positive pressure assembly 30 includes a positive pressure seat 32 having a plurality of mounting spaces 32a, part of the mounting space 32a forms the air inlet cavity 31b, the air inlet unit 31 includes a valve shaft 311, the valve shaft 311 is movably arranged in the mounting space 32a one by one to form the air outlet cavity 31c in the mounting space 32a and the positive pressure seat 32, and the valve shaft 311 is in abutment and separation with the solid phase extraction column 40 to make the air inlet unit 31 switch between the communication state and the separation state. That is, by using the movable valve shaft 311, the communication and separation of the air inlet cavity 31b and the air outlet cavity 31c can be better realized, and the state switching of the air inlet unit 31 can be realized.

[0063] Specifically, the positive pressure seat 32 has a plurality of installation spaces 32a inside, each of which corresponds to an installation of a valve shaft 311. In fact, for the positive pressure assembly 30, each installation space 32a and the corresponding valve shaft 311 form at least part of an air inlet unit 31.

[0064] In the installation space 32a, a part of the area forms an air inlet cavity 31b, and another part of the area (the space between the valve shaft 311 and the positive pressure seat 32) forms an air outlet cavity 31c. The valve shaft 311 is arranged in the installation space 32a and can move relative to the positive pressure seat 32.

[0065] When the valve shaft 311 is not in contact with the solid phase extraction column 40 (i.e., the two are separated), the valve shaft 311 is in an initial position that can separate (not communicate) the air inlet cavity 31b and the air outlet cavity 31c, and the air inlet unit 31 is in a separated state. When the valve shaft 311 gradually comes into contact with the solid phase extraction column 40, the valve shaft 311 moves relative to the positive pressure seat 32 under the abutting action of the solid phase extraction column 40, thereby enabling the air inlet cavity 31b and the air outlet cavity 31c to communicate, and the air inlet unit 31 is in a connected state.

[0066] It should be noted that the specific formation of the air flow path in the positive pressure assembly 30 can be set according to actual conditions.

[0067] For example, please refer to Figures 7 to 11 , the end of the valve shaft 311 away from the air inlet cavity 31b has an abutting end 312 extending out of the installation space 32a for abutting with the solid phase extraction column 40, the valve shaft 311 has a flow passage 311a extending in the extending direction, and the abutting end 312 has an air outlet 31a. The opposite ends of the flow passage 311a are respectively communicated with the air outlet cavity 31c and the air outlet 31a. In this way, by flowing inside the valve shaft 311, the air flow path can be sealed, and the air can be better introduced into the solid phase extraction column 40.

[0068] Specifically, the valve shaft 311 is arranged by the abutting end 312 extending out of the installation space 32a to abut with the solid phase extraction column 40, and the air outlet 31a of the abutting end 312 is communicated with the solid phase extraction column 40.

[0069] The valve shaft 311 is hollow to form a flow passage 311a extending in the axial direction of the valve shaft 311 inside. The flow passage 311a communicates the air outlet cavity 31c and the air outlet 31a, thereby avoiding leakage of gas flowing from the air outlet cavity 31c to the air outlet 31a.

[0070] In a specific embodiment, please refer to Figures 7 to 11, the valve shaft 311 has an insertion end 313, a valve rod section 314, and an abutting end 312, the valve rod section 314 is located between the insertion end 313 and the abutting end 312, and the gas outlet cavity 31c is located on the side of the valve rod section 314 close to the insertion end 313. In this way, the length of the flow passage 311a in the valve shaft 311 can be greatly extended, and the sealing performance of the gas flow path for the gas flow can be greatly improved.

[0071] Of course, in other embodiments, the gas can also enter through the outside of the valve shaft 311.

[0072] For example, the installation space 32a includes an installation channel 32b in communication with the gas inlet cavity 31b, the installation channel 32b has an installation opening, the valve shaft 311 is movably arranged in the installation channel 32b, and the installation channel 32b is spaced from the wall of the installation channel 32b to form the gas outlet cavity 31c and the flow passage 311a, the opening size of the installation opening is greater than the cross-sectional size of the valve shaft 311, to form the gas outlet 31a, and the gas outlet cavity 31c is in communication with the gas outlet 31a through the flow passage 311a. In this way, by using the gas passing through the outside of the valve shaft 311, the flow passage 311a in the valve shaft 311 does not need to be machined.

[0073] In an embodiment, referring to Figures 7 to 11 , the valve shaft 311 has an insertion end 313, a valve rod section 314, and an abutting end 312, the valve rod section 314 is located between the insertion end 313 and the abutting end 312, another part of the installation space 32a forms an installation channel 32b, the valve shaft 311 is movably arranged in the installation channel 32b, and part of the outer side wall of the valve rod section 314 is recessed to form the gas outlet cavity 31c with the wall of the installation channel 32b, and the insertion end 313 extends into the gas inlet cavity 31b. In this way, by moving the valve shaft 311, the gas inlet cavity 31b and the gas outlet cavity 31c can be communicated, and the state switching of the gas inlet unit 31 can be realized.

[0074] Specifically, the insertion end 313 of the valve shaft 311 is one end of the valve shaft 311 away from the abutting end 312, which extends into the inside of the gas inlet cavity 31b.

[0075] The valve rod section 314 of the valve shaft 311 is the shaft rod region located between the insertion end 313 and the abutting end 312, and the opposite ends thereof are connected with the insertion end 313 and the abutting end 312, respectively.

[0076] The specific structure of the valve shaft 311 can be set according to actual conditions.

[0077] For example, the insertion end 313 of the valve shaft 311 is a screw, which is tightly connected at one end of the valve rod section 314 away from the abutting end 312.

[0078] The valve shaft 311 is movable relative to the mounting channel 32b. In fact, the valve stem section 314 of the valve shaft 311 is located in the mounting channel 32b. Since a part of the outer wall of the valve stem section 314 is recessed relative to other parts, a gap space is formed between this part and the mounting channel 32b, which is the air outlet chamber 31c.

[0079] When the intake unit 31 is in the separated state, this space (i.e., the outlet chamber 31c) is not connected to the intake chamber 31b on the side of the extension end 313. As the valve shaft 311 moves toward the side closer to the intake chamber 31b, this space (i.e., the outlet chamber 31c) will also move toward the side closer to the intake chamber 31b until it is connected to the intake chamber 31b.

[0080] It should be noted that the shape of the recessed area on the outer wall of the valve stem section 314 and the connection method with the air outlet 31a can be set according to the actual situation.

[0081] For example, in the recessed area of ​​the outer wall of the valve stem section 314, the depth of the recess first increases and then decreases from top to bottom. The connection between the flow passage 311a and the intake chamber 31b is located at the maximum recessed depth of the recessed area, thereby improving the airflow effect.

[0082] In one embodiment, please refer to Figure 7 and Figure 8 When the contact end 312 separates from the solid-phase extraction column 40, at least one of the extension end 313 and the valve stem section 314 blocks the installation channel 32b, thereby separating the inlet chamber 31b and the outlet chamber 31c. When the contact end 312 abuts against the solid-phase extraction column 40, the valve shaft 311 moves towards the side closer to the inlet chamber 31b, thereby connecting the inlet chamber 31b and the outlet chamber 31c. Thus, the separation and connection between the inlet chamber 31b and the outlet chamber 31c can be effectively achieved.

[0083] Specifically, the valve shaft 311 can block the mounting channel 32b through the insertion end 313. For example, the cross-sectional dimension of the insertion end 313 is larger than the cross-sectional dimension of the mounting channel 32b, and the mounting channel 32b is blocked by the insertion end 313 fitting against the end face of the mounting channel 32b.

[0084] Depending on the actual situation, the valve shaft 311 can also block the installation channel 32b through the valve stem section 314. For example, the cross-sectional dimensions of the area where the valve stem section 314 connects with the extension end 313 are the same as the cross-sectional dimensions of the installation channel 32b, or the area where the valve stem section 314 connects with the extension end 313 is interference-fitted with the installation channel 32b to achieve the blocking of the installation channel 32b.

[0085] Of course, the valve shaft 311 can also be blocked by the insertion end 313 and the valve stem segment 314 together.

[0086] When the abutting end 312 is separated from the solid phase extraction column 40, under the blocking effect of at least one of the insertion end 313 and the valve stem segment 314, the air inlet cavity 31b and the air outlet cavity 31c are separated and not communicated. When the abutting end 312 abuts against the solid phase extraction column 40, under the abutting effect of the solid phase extraction column 40, the valve shaft 311 moves towards the side close to the air inlet cavity 31b, so that the air outlet cavity 31c gradually approaches the air inlet cavity 31b until the two are in communication.

[0087] In an embodiment, referring to Figure 10 and Figure 12 , the cross-sectional size of the air inlet cavity 31b is greater than that of the installation channel 32b, so as to form a first limiting step 321 at the communication position; the cross-sectional size of the insertion end 313 is greater than that of the valve stem segment 314, so as to form a second limiting step 315 at the joint position; when the air inlet unit 31 is in the separated state, the first limiting step 321 and the second limiting step 315 correspondingly abut against each other; when the air inlet unit 31 is in the communicated state, the first limiting step 321 and the second limiting step 315 are separated from each other. In this way, on the one hand, the blocking effect on the installation channel 32b can be greatly improved, and on the other hand, the valve shaft 311 can be prevented from being separated from the installation channel 32b.

[0088] Specifically, the installation space 32a of the positive pressure seat 32 includes the air inlet cavity 31b and the installation channel 32b, one end of the installation channel 32b communicates with the air inlet cavity 31b, and the other end is open.

[0089] Since the cross-sectional size of the air inlet cavity 31b is greater than that of the installation channel 32b, a first limiting step 321 can be formed at the position where the two are communicated. At the same time, the cross-sectional size of the insertion end 313 located in the air inlet cavity 31b is greater than that of the valve stem segment 314 located in the installation channel 32b, so that a second limiting step 315 can be formed at the joint position of the two.

[0090] It can be understood that when the abutting end 312 is separated from the solid phase extraction column 40, i.e. when the air inlet unit 31 is in the separated state, the first limiting step 321 and the second limiting step 315 correspondingly abut against each other, so as to block the communication position between the installation channel 32b and the air inlet cavity 31b, and to limit the valve shaft 311 from being separated from the positive pressure seat 32.

[0091] When the abutting end 312 abuts against the solid phase extraction column 40, i.e. when the air inlet unit 31 is in the separated state, the first limiting step 321 and the second limiting step 315 are separated from each other, so that the air inlet cavity 31b and the air outlet cavity 31c are communicated.

[0092] In an embodiment, referring to Figures 6 to 11 The positive pressure assembly 30 further comprises a first sealing member 33, which is arranged at one end of the mounting channel 32b close to the air inlet cavity 31b and at one side of the air outlet cavity 31c close to the air inlet cavity 31b. In this way, the sealing performance between the air outlet cavity 31c and the air inlet cavity 31b can be greatly improved, and it can be ensured that the air outlet cavity 31c and the air inlet cavity 31b are not connected in the case that the abutting end 312 is separated from the solid phase extraction column 40.

[0093] It should be noted that the type and arrangement of the first sealing member 33 can be set according to actual conditions.

[0094] For example, the first sealing member 33 is a sealing ring, and a part of the wall of the mounting channel 32b is recessed to form a mounting groove, the first sealing member 33 is clamped in the mounting groove and is sleeved on the valve shaft 311.

[0095] In an embodiment, referring to Figures 6 to 11 The positive pressure assembly 30 further comprises a second sealing member 331, which is arranged at one end of the mounting channel 32b away from the air inlet cavity 31b and at one side of the air outlet cavity 31c away from the air inlet cavity 31b. In this way, the sealing performance between the air outlet cavity 31c and the external environment can be greatly improved, and it can be ensured that the gas in the air outlet cavity 31c will not leak through the lower end opening of the mounting channel 32b.

[0096] It should be noted that the type and arrangement of the second sealing member 331 can be set according to actual conditions.

[0097] For example, the second sealing member 331 is a sealing ring, and a part of the wall of the mounting channel 32b is recessed to form a mounting groove, the second sealing member 331 is clamped in the mounting groove and is sleeved on the valve shaft 311.

[0098] In an embodiment, referring to Figures 6 to 11 The positive pressure assembly 30 further comprises a third sealing member 332, which is arranged at the joint between the abutting end 312 and the solid phase extraction column 40 when the abutting end 312 abuts against the solid phase extraction column 40 and blocks the opening of the solid phase extraction column 40.

[0099] Specifically, the opening of the solid phase extraction column 40 can allow the abutting end 312 of the valve shaft 311 to extend into it, so that the gas can enter the solid phase extraction column 40 through the valve shaft 311. Therefore, the third sealing member 332 arranged here can improve the sealing performance in the solid phase extraction column 40 and can prevent the gas from leaking from the connection between the valve shaft 311 and the solid phase extraction column 40.

[0100] It should be noted that the type and arrangement of the third sealing member 332 can be set according to actual conditions.

[0101] For example, the third seal 332 is a sealing ring, a part of the outer side wall of the abutting end 312 is recessed to form a mounting groove, and the third seal 332 is clamped in the mounting groove.

[0102] In an embodiment, referring to Figures 6 to 11 , a part of the outer surface of the abutting end 312 is protruded to form a protruding part 3121, and the protruding part 3121 is used to abut against the outer end surface of the solid-phase extraction column 40; the positive pressure assembly 30 further comprises a first elastic member 34, which is sleeved on the valve shaft 311 and abuts against the protruding part 3121 and the positive pressure seat 32 at opposite ends, respectively.

[0103] Specifically, when the air inlet unit 31 abuts against the solid-phase extraction column 40 in correspondence, the protruding part 3121 abuts against the outer end surface of the solid-phase extraction column 40, thereby being able to limit the valve shaft 311 to continue moving towards the side close to the solid-phase extraction column 40. With the positive pressure assembly 30 and the solid-phase extraction column 40 moving close to each other, the solid-phase extraction column 40 is able to push the valve shaft 311 upward in reverse, and further make the air inlet cavity 31b and the air outlet cavity 31c communicate with each other.

[0104] Meanwhile, the protruding part 3121 can also be used for the first elastic member 34 to abut against. By arranging the first elastic member 34, on the one hand, the valve shaft 311 is able to move towards the side away from the air inlet cavity 31b under the elastic force of the first elastic member 34 in the process of switching the air inlet unit 31 from abutting against the solid-phase extraction column 40 to separating from each other, so as to reset to the initial position of making the air inlet cavity 31b and the air outlet cavity 31c re-separated. On the other hand, the abutting force can always be applied to the valve shaft 311 when the abutting end 312 separates from the solid-phase extraction column 40, so as to prevent the valve shaft 311 from loosening, thereby being able to improve the sealing effect between the air inlet cavity 31b and the air outlet cavity 31c.

[0105] In an embodiment, the positive pressure assembly 30 further comprises a second elastic member, which is arranged in the air inlet cavity 31b and abuts against the protruding end 313 and the positive pressure seat 32 at opposite ends, respectively. By arranging the second elastic member in the air inlet cavity 31b, on the one hand, the valve shaft 311 is facilitated to reset, and on the other hand, the abutting force can always be applied to the valve shaft 311 when the abutting end 312 separates from the solid-phase extraction column 40, so as to prevent the valve shaft 311 from loosening. Meanwhile, the protruding end 313 is also prevented from directly impacting or directly contacting the cavity wall of the air inlet cavity 31b.

[0106] In an embodiment, referring to Figures 6 to 11The positive pressure assembly 30 further comprises a baffle 35, a guide column 36 and a third elastic member 341. The guide column 36 is arranged on the positive pressure seat 32 close to the hole plate 20. The baffle 35 is slidably sleeved on the guide column 36. The third elastic member 341 is sleeved on the guide column 36 and abuts against the baffle 35 and the positive pressure seat 32 respectively. The baffle 35 has a plurality of through holes 35a. The end of the valve shaft 311 away from the air inlet cavity 31b has an abutting end 312 which is correspondingly arranged in the through hole 35a. The baffle 35 is arranged to abut against the outer end surface of the solid phase extraction column 40. By arranging the baffle 35, the solid phase extraction column 40 can be compressed during the switching of the air inlet unit 31 from the communication state to the separation state, and the solid phase extraction column 40 can be prevented from being separated from the hole plate 20 together with the valve shaft 311.

[0107] Specifically, when the air inlet unit 31 does not abut against the solid phase extraction column 40, the baffle 35 is limited at the lower end of the guide column 36 under the action of the third elastic member 341 and the self weight.

[0108] During the movement of the positive pressure assembly 30 towards the side close to the solid phase extraction column 40, the baffle 35 is in contact with the solid phase extraction column 40, and the solid phase extraction column 40 pushes the baffle 35 to move towards the side of the positive pressure seat 32. The third elastic member 341 arranged between the baffle 35 and the positive pressure seat 32 is compressed, the solid phase extraction column 40 abuts against the valve shaft 311, and the air inlet cavity 31b and the air outlet cavity 31c are communicated.

[0109] When the air supply into the solid phase extraction column 40 is completed, the positive pressure assembly 30 moves towards the side away from the solid phase extraction column 40. The baffle 35 compresses the solid phase extraction column 40 under the abutting action of the third elastic member 341, so that the solid phase extraction column 40 can be compressed on the hole plate 20, the valve shaft 311 gradually separates from the solid phase extraction column 40, and the solid phase extraction column 40 will not be separated from the hole plate 20.

[0110] It can be understood that the plurality of through holes 35a on the baffle 35 correspond to the number of valve shafts 311, so that the valve shaft 311 can pass through and abut against the corresponding solid phase extraction column 40.

[0111] In an embodiment, as shown in Figures 6 to 8 When the air inlet unit 31 is separated from the solid phase extraction column 40, the lower end surface of the abutting end 312 is located in the through hole 35a. Thus, the baffle 35 can always compress the solid phase extraction column 40 during the separation of the valve shaft 311 and the solid phase extraction column 40, so as to achieve a better compression effect.

[0112] In an embodiment, as shown in Figures 6 to 11 The positive pressure assembly 30 can be lifted and lowered relative to the seat body 10.

[0113] In the initial position, the positive pressure assembly 30 is spaced apart from the hole plate 20 in the up-down direction. As the positive pressure assembly 30 gradually descends, the baffle 35 first abuts against the solid phase extraction column 40 on the hole plate 20, and the third elastic member 341 is compressed.

[0114] As the positive pressure assembly 30 gradually descends, the valve shaft 311 also abuts against the solid phase extraction column 40, and the solid phase extraction column 40 pushes the valve shaft 311 relative to the positive pressure seat 32 towards the side close to the air inlet cavity 31b, so as to make the air inlet cavity 31b and the air outlet cavity 31c communicate. When the positive pressure assembly 30 ascends, the valve shaft 311 moves relative to the positive pressure seat 32 towards the side away from the air inlet cavity 31b under the elastic action of the first elastic member 34, so as to make the air inlet cavity 31b and the air outlet cavity 31c re-divide. As the positive pressure assembly 30 gradually ascends, the valve shaft 311 is separated from the solid phase extraction column 40, and the solid phase extraction column 40 cannot be separated from the hole plate 20 under the compression of the baffle 35.

[0115] It can be understood that for the mounting hole 20a without the solid phase extraction column 40, since the valve shaft 311 at the corresponding position does not abut against the solid phase extraction column 40, the valve shaft 311 and the positive pressure seat 32 will not produce relative movement, thereby enabling the air inlet cavity 31b and the air outlet cavity 31c to always be in a divided state, and thus being able to prevent gas leakage.

[0116] In an embodiment, please refer to Figure 2 , Figure 13 and Figure 14 , the positive pressure column device further comprises a temperature control assembly 50, which is arranged on the seat body 10 in a liftable manner, and has a temperature control cavity 50a with an opening downward. The temperature control assembly 50 is lifted to switch the hole plate 20 between entering and leaving the temperature control cavity 50a. In this way, the hole plate 20 can be conveniently loaded into the temperature control assembly 50, and the structure of the temperature control assembly 50 can be simplified.

[0117] Specifically, the temperature control assembly 50 can be used to heat and control the temperature of the solid phase extraction column 40 mounted on the hole plate 20, and can start heating according to the set parameters, and keep the temperature within the interval range when heated to the set temperature.

[0118] The bottom of the temperature control cavity 50a forms an opening, and by lifting the temperature control assembly 50, the hole plate 20 and the solid phase extraction column 40 on the hole plate 20 can enter the temperature control cavity 50a.

[0119] It should be noted that the specific structure of the temperature control assembly 50 can be set according to actual conditions.

[0120] For example, please refer to Figure 13 and Figure 14The temperature control assembly 50 comprises a silica gel heating pad 51, a heat dissipation plate 52 and a temperature control seat 53. The temperature control seat 53 has a temperature control cavity 50a. The silica gel heating pad 51 and the heat dissipation plate 52 are arranged in the temperature control cavity 50a. The silica gel heating pad 51 is arranged on the side of the heat dissipation plate 52 away from the hole plate 20. In this way, the solid phase extraction column 40 can be heated and temperature controlled well.

[0121] Specifically, the silica gel heating pad 51 can convert electrical energy into heat energy. According to actual conditions, a temperature sensor can also be arranged in the silica gel heating pad 51 to feed back real-time temperature. The heating power is adjusted according to the real-time temperature and the predetermined temperature until the predetermined temperature is reached and maintained.

[0122] The heat dissipation plate 52 has good heat transfer effect. The heat can be distributed more evenly in the temperature control cavity 50a.

[0123] In an embodiment, referring to Figure 1 and Figure 2 The positive pressure column passing device comprises a lifting assembly 60 and a translation assembly 70. The lifting assembly 60 is arranged on the seat body 10. The temperature control assembly 50 and the positive pressure assembly 30 are arranged on the lifting assembly 60 along the first direction, so as to be lifted up and down relative to the seat body 10 through the lifting assembly 60. The translation assembly 70 is slidably arranged on the seat body 10 along the first direction. The hole plate 20 is arranged on the translation assembly 70, so as to be moved relative to the seat body 10 through the translation assembly 70. In this way, through the translation function of the translation assembly 70 and the lifting function of the lifting assembly 60, the solid phase extraction column 40 can be well matched with the temperature control assembly 50 and the positive pressure assembly 30 respectively.

[0124] Specifically, the hole plate 20 and the solid phase extraction column 40 can be moved to the lower side of the positive pressure assembly 30 along the first direction through the translation assembly 70. The positive pressure assembly 30 can be lifted to perform positive pressure column passing operation on the solid phase extraction column 40 through the lifting assembly 60.

[0125] When the solid phase extraction column 40 needs to be heated and temperature controlled through the temperature control assembly 50, the hole plate 20 and the solid phase extraction column 40 can be moved to the lower side of the temperature control assembly 50 along the first direction through the translation assembly 70. The temperature control assembly 50 can be lifted through the lifting assembly 60 to heat and temperature control the solid phase extraction column 40.

[0126] In an embodiment, the positive pressure column device further comprises a first positioning structure and a second positioning structure. The first positioning structure is arranged on the seat body 10 and located at the bottom side of the positive pressure assembly 30. The first positioning structure is used for positioning cooperation with the translation assembly 70, so that the translation assembly 70 can be better positioned at the lower side of the positive pressure assembly 30 during movement in the first direction. The second positioning structure is arranged on the seat body 10 and located at the bottom side of the temperature control assembly 50. The second positioning structure is used for positioning cooperation with the translation assembly 70, so that the translation assembly 70 can be better positioned at the lower side of the temperature control assembly 50 during movement in the first direction.

[0127] It should be noted that the specific structure of the first positioning structure and the second positioning structure is not limited. For example, a buckle structure or a magnetic attraction structure.

[0128] It should be noted that the specific structure of the lifting assembly 60 can be set according to actual conditions.

[0129] In an embodiment, referring to Figure 1 and Figure 3 , the positive pressure column device comprises a lifting assembly 60, the lifting assembly 60 comprises an upper support plate 61, a lifting support plate 62, a synchronous belt pulley structure 63, a driving motor 64, a ball screw nut 65 and a ball screw 66, the ball screw 66 is rotatably arranged on the seat body 10, the upper support plate 61 is sleeved on the ball screw 66, the lifting support plate 62 is sleeved on the ball screw 66 through the ball screw nut 65, the positive pressure assembly 30 is arranged on the lifting support plate 62, the driving motor 64 and the synchronous belt pulley structure 63 are arranged on the upper support plate 61, and the driving motor 64 is drivingly connected with the ball screw 66 through the synchronous belt pulley structure 63. Thus, by starting the driving motor 64, the positive pressure assembly 30 can be driven to lift as a whole, and the stability of the lifting of the positive pressure assembly 30 as a whole can be improved.

[0130] Specifically, the upper support plate 61 is used for fixedly mounting the driving motor 64 and the synchronous belt pulley structure 63, and the lifting support plate 62 is used for mounting the positive pressure assembly 30. The ball screw 66 passes through the upper support plate 61, the lifting support plate 62 and the seat body 10 in sequence and is rotatable relative to the three. The lifting support plate 62 and the ball screw 66 are connected through the ball screw nut 65. Thus, by cooperation of the ball screw 66 and the ball screw nut 65, rotation can be converted into linear motion, so that the ball screw nut 65 and the lifting support plate 62 move axially along the ball screw 66, thereby driving the positive pressure assembly 30 to lift.

[0131] According to actual conditions, the temperature control assembly 50 can also be arranged on the lifting support plate 62, so that the lifting support plate 62 can drive the temperature control assembly 50 and the positive pressure assembly 30 to lift at the same time.

[0132] It should be noted that the specific number of the ball screws 66 can be set according to actual conditions.

[0133] For example, the lifting assembly 60 includes two ball screws 66 and two rotating shafts, which are also arranged through the upper support plate 61, the lifting support plate 62 and the seat body 10.

[0134] The specific structure of the synchronous belt wheel structure 63 can be set according to actual conditions.

[0135] For example, referring to Figure 1 and Figure 3 , the synchronous belt wheel structure 63 includes a plurality of synchronous belt wheels 631 and a synchronous belt 632, the synchronous belt wheels 631 are rotatably arranged on the upper support plate 61, the driving motor 64 is drivingly connected with one of the synchronous belt wheels 631, and the end of each of the ball screws 66 is provided with one of the synchronous belt wheels 631, and each of the synchronous belt wheels 631 is drivingly connected through the synchronous belt 632.

[0136] In some embodiments, referring to Figure 1 and Figure 3 , the synchronous belt wheel structure 63 further includes a tension pulley 633 and an idler pulley 634, which are respectively drivingly connected with the synchronous belt 632.

[0137] The specific structure of the translation assembly 70 can be set according to actual conditions.

[0138] For example, referring to Figure 2 , the translation assembly 70 includes a linear guide rail 71, a lead screw motor 72, a lead screw nut 73, a sliding table adapter 74, a bearing seat 75 and a motor seat 76. The bearing seat 75 and the motor seat 76 are arranged on the seat body 10 along the first direction, the lead screw motor 72 is arranged on the motor seat 76, and the lead screw of the lead screw motor 72 extends to the bearing seat 75 along the first direction and can rotate relative to the bearing seat 75 and the motor seat 76. The linear guide rail 71 is located on the lower side of the lead screw, the hole plate 20 is arranged on the sliding table adapter 74, the sliding table adapter 74 is slidingly arranged on the linear guide rail 71, and the sliding table adapter 74 is connected with the lead screw of the lead screw motor 72 through the lead screw nut 73. Thus, by driving the lead screw nut 73 to move through the lead screw motor 72, the sliding table adapter 74 and the hole plate 20 can be driven to move along the first direction.

[0139] In a specific embodiment, the translation assembly 70 includes the sliding table adapter 74, when the hole plate 20 is located in the temperature control cavity 50a, the sliding table adapter 74 seals the lower opening of the temperature control cavity 50a. The sliding table adapter 74 is circumferentially formed with a clamping groove around the hole plate 20, and the wall body at the lower opening of the temperature control cavity 50a is clamped into the clamping groove, thereby improving the sealing and heat preservation performance of the temperature control cavity 50a.

[0140] In the description of the application, the description of the terms "in an embodiment", "in some embodiments", "in a specific embodiment", or "exemplary" and the like means that the specific feature, structure, material or characteristic being described is included in at least one embodiment or example of the application. The illustrative appearance of the above-mentioned terms in various places in the specification is not necessarily intended to refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics can be combined in any suitable manner in one or more embodiments or examples. Furthermore, the different embodiments or examples described in the application and the features of the different embodiments or examples can be combined with each other, if not mutually exclusive.

[0141] The above only describes the preferred embodiments of the application and is not intended to limit the application. The application can have various modifications and changes for those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the application is included in the protection scope of the application.

Claims

1. A positive pressure over column apparatus, characterized by, The device comprises a seat body, a hole plate and a positive pressure assembly. The hole plate is arranged on the seat body and has a plurality of mounting holes for accommodating solid phase extraction columns. The positive pressure assembly is arranged on the seat body and spaced from the seat body to form a positive pressure space for accommodating the hole plate. The positive pressure assembly has an air inlet and a plurality of air inlet units, each of which comprises an air outlet, an air inlet cavity and an air outlet cavity. The air inlet unit has a communication state and a separation state. When the air inlet unit is in abutment with the solid phase extraction column, the air inlet cavity and the air outlet cavity are in communication. The positive pressure assembly comprises a positive pressure seat having a plurality of mounting spaces. The valve shaft has an abutment end extending out of the mounting space for abutting with the solid phase extraction column.

2. The positive pressure over column apparatus of claim 1, wherein, The valve shaft further has a protruding end and a valve stem segment. The valve stem segment is located between the protruding end and the abutment end. The positive pressure assembly further comprises a baffle, a guide column and a third elastic member. When the abutment end is separated from the solid phase extraction column, at least one of the protruding end and the valve stem segment blocks the mounting channel to separate the air inlet cavity and the air outlet cavity. When the abutment end is in abutment with the solid phase extraction column, the valve shaft moves towards the side close to the air inlet cavity to make the air inlet cavity and the air outlet cavity in communication.

3. The positive pressure over column apparatus of claim 1, wherein, The cross-sectional size of the air inlet cavity is larger than that of the mounting channel to form a first limiting step at the communication position; the cross-sectional size of the protruding end is larger than that of the valve stem segment to form a second limiting step at the connection position; the first limiting step and the second limiting step correspondingly abut when the air inlet unit is in the separated state; the first limiting step and the second limiting step are separated from each other when the air inlet unit is in the communication state.

4. The positive pressure over column apparatus of claim 1, wherein, The positive pressure assembly further comprises a first sealing member, which is arranged at one end of the mounting channel close to the air inlet cavity and at one side of the air outlet cavity close to the air inlet cavity; and / or, The positive pressure assembly further comprises a second sealing member, which is arranged at one end of the mounting channel away from the air inlet cavity and at one side of the air outlet cavity away from the air inlet cavity; and / or, The positive pressure assembly further comprises a third sealing member, which is arranged at the abutting end and the solid-phase extraction column when the abutting end abuts against the solid-phase extraction column, and the abutting end blocks the opening of the solid-phase extraction column.

5. The positive pressure over column apparatus of claim 1, wherein, Part of the outer surface of the abutting end is protruded to form a protruding part, which is used to abut against the outer end surface of the solid-phase extraction column; the positive pressure assembly further comprises a first elastic member, which is sleeved on the valve shaft and abuts against the protruding part and the positive pressure seat at opposite ends; and / or, The positive pressure assembly further comprises a second elastic member, which is arranged in the air inlet cavity and abuts against the protruding end and the positive pressure seat at opposite ends.

6. The positive pressure over column apparatus of claim 1, wherein, The guide column is arranged at one side of the positive pressure seat close to the hole plate, the baffle is slidably sleeved on the guide column, the third elastic member is sleeved on the guide column and abuts against the baffle and the positive pressure seat, the baffle has a plurality of through holes, one end of the valve shaft away from the air inlet cavity has an abutting end, the abutting end is correspondingly arranged in the through hole, and the baffle is used to abut against the outer end surface of the solid-phase extraction column.

7. The positive pressure over column apparatus of claim 6, wherein, When the air inlet unit is separated from the solid-phase extraction column, the lower end surface of the abutting end is located in the through hole.

8. The positive pressure over column apparatus of any one of claims 1-7, wherein, The positive pressure column device further comprises a temperature control assembly, which is arranged on the seat body in a lifting manner, has a temperature control cavity with an opening downward, and is switched between entering and leaving the temperature control cavity by lifting.

9. The positive pressure over column apparatus of claim 8, wherein, The temperature control assembly comprises a silica gel heating pad, a heat dissipation plate and a temperature control seat, the temperature control seat has the temperature control cavity, the silica gel heating pad and the heat dissipation plate are arranged in the temperature control cavity, and the silica gel heating pad is arranged at one side of the heat dissipation plate away from the hole plate.

10. The positive pressure over column apparatus of claim 8, wherein, The positive pressure column device comprises a lifting assembly and a translation assembly, the lifting assembly is arranged on the seat body, the temperature control assembly and the positive pressure assembly are arranged on the lifting assembly along a first direction to be lifted up and down relative to the seat body through the lifting assembly, and the translation assembly is slidably arranged on the seat body along the first direction, and the hole plate is arranged on the translation assembly to move relative to the seat body through the translation assembly.

11. The positive pressure over column apparatus of any one of claims 1-7, wherein, The positive pressure column device comprises a lifting assembly, the lifting assembly comprises an upper supporting plate, a lifting supporting plate, a synchronous belt wheel structure, a driving motor, a ball screw nut and a ball screw, the ball screw is rotatably arranged on the seat body, the upper supporting plate is sleeved on the ball screw, the lifting supporting plate is sleeved on the ball screw through the ball screw nut, the positive pressure assembly is arranged on the lifting supporting plate, the driving motor and the synchronous belt wheel structure are arranged on the upper supporting plate, and the driving motor is drivingly connected with the ball screw through the synchronous belt wheel structure to drive the ball screw to rotate, so that the lifting supporting plate drives the positive pressure assembly to lift.

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