Pure natural bacteriostatic anti-allergic olive oil handmade soap and preparation process thereof

Abstract

The handmade soap contains a lipidosome nano-emulsion compound system, has good film-forming property, can protect sensitive components, can encapsulate active components in traditional Chinese medicinal material extracts which are easy to oxidize, and can resist a strong alkaline environment of the medicated soap, so that the handmade soap has a good anti-allergy effect, and the handmade soap has a good anti-allergy effect and a good anti-allergy effect. The nano-emulsion increases fat-soluble components, improves bioavailability and promotes skin absorption, and the nano-scale particle size (100-200nm) of the liposome and the nano-emulsion can penetrate through the cuticle, so that slow-release delivery is realized, and the drug effect is prolonged. Meanwhile, an artificial moisturizing barrier can be constructed for the skin, and moisture evaporation on the surface of the skin is reduced; the traditional Chinese medicinal material extract has relatively strong bacteriostatic activity and very strong antioxidant activity on pathogenic bacteria and pathogenic fungi. Therefore, the handmade soap disclosed by the invention has the effects of moisturizing, cleaning, inhibiting bacteria, resisting inflammation, resisting allergy and the like, can better protect fragile skin, and can help wounded skin heal at the same time.

Description

Technical Field

[0001] This invention belongs to the field of handmade soap preparation technology, specifically a pure natural antibacterial and anti-allergic handmade soap containing a compound extract of Chinese medicinal herbs and its preparation process. Background Technology

[0002] Handmade soap is a traditional detergent made by hand using oils and lye through a saponification reaction. The main preparation process involves curing and maturation. It can be used for washing and cleaning. Based on its function, it can be categorized into medicated soap, makeup remover soap, and functional soap. Soaps made primarily from sodium fatty acids and other surfactants, with added quality and appearance improvers, are processed and shaped. Due to their high pH value, skin cannot tolerate them for extended periods, so they are only suitable for washing clothes.

[0003] Currently available plant-based mild alkaline soaps have a low pH, making them easily tolerated by human skin over a long period. The various nutrients and vitamins contained in plants provide nourishment and care for the skin, offering a relatively gentle cleansing effect. However, their functions are limited, primarily focusing on cleansing and moisturizing. With rising living standards, people have higher expectations for the efficacy of handmade soaps. For example, they desire soaps that not only moisturize and nourish but also possess anti-inflammatory, antibacterial, and even anti-allergic properties. Currently, there are no reports of handmade soaps possessing all of these functions. Summary of the Invention

[0004] Based on the above, the purpose of this invention is to provide a pure natural antibacterial and anti-allergic handmade soap containing a compound extract of traditional Chinese medicine and its preparation process, so that the handmade soap has both moisturizing and anti-inflammatory and antibacterial effects, in order to meet people's needs.

[0005] To achieve its purpose, the present invention adopts the following technical solution:

[0006] This invention provides a pure natural antibacterial and anti-allergic olive oil handmade soap, which is made from the following raw materials by weight:

[0007] The mixture consists of 10-50 parts of a liposome-nanoemulsion complex system, 60-100 parts of olive oil, 0.5-5 parts of alkali solution, 0.5-2 parts of olive leaf extract, 6-9 parts of a complex Chinese herbal medicine extract, 2-3 parts of essential oil, and 50-70 parts of Tween-20.

[0008] As a further preferred embodiment of the technical solution of the present invention, the alkaline solution is a sodium hydroxide solution with a concentration of 0.2 to 0.6 g / mL.

[0009] Preferably, the liposome-nanoemulsion composite system is prepared as follows:

[0010] The liposome suspension and nanoemulsion were mixed at a volume ratio of 1:1 to 5 and stirred at 200 rpm for 15 to 45 minutes to form a composite system.

[0011] Preferably, the liposome suspension is prepared as follows:

[0012] (1) Film formation: Dissolve 0.3g soybean lecithin, 0.1g cholesterol and 0.1g oat alkaloids in 10mL chloroform and evaporate at 50℃ for 10min to form a homogeneous film;

[0013] (2) Hydration: 15 mL of pH 7.0 phosphate buffer was added to the homogenized film in step (1), and after hydration, it was ultrasonically treated to control the particle size to 100-200 nm.

[0014] (3) Purification: High-speed centrifugation was used to remove unencapsulated components to prepare the liposome suspension.

[0015] Preferably, the nanoemulsion is prepared as follows:

[0016] (1) Primary emulsification: Mix 5g of olive oil with 1g of emulsifier (Tween-20), slowly add 40mL of water, and stir at 1000rpm for 10min to form a coarse emulsion;

[0017] (2) High-pressure homogenization: Use a homogenizer to circulate the liquid until the droplet size is <200nm;

[0018] (3) Stability treatment: Add 1% vitamin E 0.06g and store at 4℃.

[0019] Preferably, the essential oil is 2-3 parts sweet orange essential oil or 1-2 parts osmanthus essential oil.

[0020] Preferably, the compound Chinese herbal extract is obtained by mixing extracts of Saposhnikovia divaricata, Sophora flavescens, Scutellaria baicalensis, Kochia scoparia, Dictamnus dasycarpus, and Glycyrrhiza uralensis, adding isooctyl palmitate at a mass-volume ratio of 1:10, and stirring thoroughly.

[0021] Preferably, the Saposhnikovia extract is obtained by pulverizing Saposhnikovia divaricata, weighing 5g, adding 200mL of 95% ethanol solution, ultrasonically extracting at 50℃ for 30min, filtering, and then reducing the pressure, concentrating, and drying the filtrate.

[0022] The Sophora flavescens extract is prepared by crushing Sophora flavescens through a 0.2mm sieve, weighing 10g of powder, adding 55% ethanol at a material-to-liquid mass-to-volume ratio of 1:10, refluxing at 80℃ for 1h, extracting twice, combining the two filtrates, concentrating and drying.

[0023] The Scutellaria baicalensis extract is prepared by pulverizing Scutellaria baicalensis through a 0.2 mm sieve, weighing 10 g of powder, adding 70% ethanol at a material-to-liquid mass-to-volume ratio of 1:10, refluxing at 80°C for 2 h, extracting twice, combining the two filtrates, and then rotary evaporating and concentrating the filtrate at 55°C.

[0024] The Kochia scoparia extract is obtained by pulverizing Kochia scoparia, weighing 5g, adding 50mL of 45% ethanol solution, extracting in a constant temperature water bath at 70℃ for 5h, filtering, and then reducing the pressure, concentrating and drying the filtrate.

[0025] The extract of Dictamnus dasycarpus root bark is prepared by crushing Dictamnus dasycarpus root bark, weighing 20g, placing it in a 250mL round-bottom flask, adding 100mL of petroleum ether and soaking for 30min, refluxing and extracting three times, each time for 2h, filtering and combining the extracts, and then concentrating and drying under reduced pressure.

[0026] The licorice extract is obtained by pulverizing licorice, weighing 5g, adding 50mL of 75% ethanol solution, ultrasonically treating for 30min, covering and soaking at room temperature for 1 week, filtering, and then reducing the pressure, concentrating and drying the filtrate.

[0027] The preparation method of the above-mentioned all-natural antibacterial and anti-allergic handmade soap includes the following steps:

[0028] (1) Dissolving alkali: Weigh the alkali substance, add deionized water, stir to dissolve, and wait for the temperature to drop to 38℃ to obtain the alkali solution;

[0029] (2) Oil preparation: Olive oil is mixed with compound Chinese herbal extracts, olive leaf extract is added, and the mixture is stirred until well mixed to obtain oil;

[0030] (3) Soap base preparation: Mix the alkaline solution obtained in (1) and the oil obtained in (2), stir, and obtain soap base;

[0031] (4) Adding the composite system: Slowly pour the liposome-nanoemulsion composite system into the soap base and gently stir until it is evenly dispersed without layering.

[0032] (5) Add essential oil and Tween-20: Add essential oil to the product obtained in (4), continue stirring, then add Tween-20 and continue stirring until Trace (the "∞" sign is clear);

[0033] (6) Molding and solidification: Pour the product obtained in (5) into the mold, gently shake it to remove air bubbles, and demold it after cooling to obtain the finished handmade soap.

[0034] By adopting the above technical solution, the beneficial effects of the present invention are as follows:

[0035] This invention relates to a handmade soap containing a liposome nanoemulsion composite system. This system possesses excellent film-forming properties, protecting sensitive ingredients and encapsulating the active ingredients in easily oxidized herbal extracts. It also resists the highly alkaline environment of medicated soaps. The nanoemulsion further increases the fat-soluble components, improving bioavailability and promoting skin absorption. The nanoscale particle size (100-200nm) of the liposomes and nanoemulsions allows them to penetrate the stratum corneum, achieving sustained-release delivery and prolonging the therapeutic effect. Simultaneously, it constructs an artificial moisturizing barrier for the skin, reducing moisture evaporation from the skin surface. The herbal extracts exhibit strong antibacterial activity against pathogenic bacteria and fungi, as well as significant antioxidant activity. Specifically:

[0036] This invention's handmade soap contains extracts from six kinds of traditional Chinese medicinal herbs. Among them, Sophora flavescens contains matrine and oxymatrine, which have the effects of inhibiting histamine release, anti-inflammation, and anti-allergy, and are suitable for diseases such as eczema, atopic dermatitis, and urticaria; Scutellaria baicalensis contains baicalin, baicalein and flavonoids, which have multiple pharmacological effects such as anti-allergy, antibacterial and anti-inflammatory, and antioxidant, and have good external effects; Kochia scoparia alcohol extract can inhibit mast cell degranulation and inhibit histamine release, and the total saponins can produce significant inhibitory effects on type I and type IV hypersensitivity reactions; Saposhnikovia divaricata has the effects of dispelling wind and cold, relieving pain and itching, and is often used to treat skin diseases caused by wind invasion, such as urticaria, sores and rashes. Modern pharmacological studies have shown that Saposhnikovia divaricata has antipyretic, anti-inflammatory, analgesic, and sedative effects, and can regulate the body's immune function and reduce allergic reactions; Dictamnus dasycarpus has the effects of clearing heat and drying dampness, dispelling wind and detoxifying, and its dictamnus alkaloid has anti-inflammatory and anti-allergic effects, and can be used to treat diseases such as eczema, urticaria, and psoriasis; Glycyrrhiza uralensis is rich in glycyrrhizin and glycyrrhetinic acid, and has the effects of inhibiting inflammatory reactions, relieving pain, expectorating phlegm and relieving cough, and anti-allergy. When respiratory allergic diseases occur, it can protect the bronchial mucosa; The compound extract composed of the above 6 Chinese medicinal materials has good safety and efficacy as a raw material for anti-inflammatory and acne-reducing effects.

[0037] Furthermore, olive oil, as a base oil, is known as "liquid gold," and this is especially evident in handmade soap products: (1) Excellent moisturizing properties: Olive oil is rich in nutrients such as oleic acid and vitamin E, which have excellent moisturizing effects. It can provide sufficient moisture and nutrients to the skin while cleansing it, keeping the skin soft and smooth; (2) Antioxidant and anti-aging properties: Antioxidants in olive oil, such as vitamin E, help reduce the production of free radicals, protect the skin from damage caused by the external environment, promote skin metabolism, reduce wrinkles, and play an anti-aging role; (3) Gentle and non-irritating: Olive oil is gentle and will not cause irritation or damage to the skin. It is suitable for all skin types, including sensitive skin and baby skin; (4) Improves the skin barrier: Handmade soap made with olive oil can enhance the skin barrier function, reduce moisture loss, and prevent harmful substances from the outside world from invading the skin, thereby protecting skin health.

[0038] Furthermore, the olive oil handmade soap obtained through the cold process of this invention not only retains various antioxidant components in the raw oil, making the soap more delicate, but also has an extremely high content of linoleic acid and oleic acid, making the soap more moisturizing. In the liposome-nanoemulsion complex system, liposomes provide sustained release and targeting, while nanoemulsions enhance permeability and stability. At the same time, the nanoemulsions rapidly penetrate to the skin surface, while the liposomes continuously release deep into the skin, thereby better cleansing and protecting the skin.

[0039] In summary, the handmade soap of this invention has antibacterial, anti-inflammatory, and anti-allergic effects, which can better protect fragile skin and help heal damaged skin. Attached Figure Description

[0040] Figure 1 This is a product diagram of Embodiment 1 of the present invention;

[0041] Figure 2 This is a product diagram from Embodiment 2 of the present invention;

[0042] Figure 3 This is a product diagram of Embodiment 3 of the present invention;

[0043] Figure 4 This is a product diagram from Embodiment 4 of the present invention;

[0044] Figure 5 This is a product diagram from Embodiment 5 of the present invention;

[0045] Figure 6 This is a product diagram of Embodiment 6 of the present invention;

[0046] Figure 7 This is a product diagram from Embodiment 7 of the present invention;

[0047] Figure 8 For comparison example 1, see the product image;

[0048] Figure 9 For comparison example 2, see the product image;

[0049] Figure 10 For comparison example 3, see the product image;

[0050] Figure 11 For comparison example 4, see the product image;

[0051] Figure 12 For comparison example 5, see product image;

[0052] Figure 13 For comparison example 6, see product image;

[0053] Figure 14 The chromatograms of 80 mixed standard samples with a concentration of 5 μg / kg determined by liquid chromatography-tandem mass spectrometry and the sample image of Example 1 are shown. Detailed Implementation

[0054] The preparation process and performance of the handmade soap of the present invention will be described in detail below through specific embodiments.

[0055] The extraction of each Chinese medicinal herb in the compound Chinese medicinal herb extract is based on the instructions.

[0056] Example 1

[0057] This embodiment provides a pure natural antibacterial and anti-allergic olive oil handmade soap, the preparation method of which is as follows:

[0058] (1) Dissolving alkali: Weigh 26g of sodium hydroxide, add 50mL of deionized water, the water releases heat after adding water, stir evenly and then cool down in a water bath to 38℃ (if it is not stirred evenly before cooling, it will cause sodium hydroxide to clump), and obtain alkali solution.

[0059] (2) Oil preparation: Weigh 80g of olive oil as the base oil, add 7g of compound Chinese herbal extract and 1g of olive leaf extract, stir for 40min to mix them thoroughly to obtain the oil;

[0060] (3) Soap base preparation: Take 0.5 mL of the alkaline solution in (1) and add it to the oil in (2), stir thoroughly to obtain soap base;

[0061] (4) Adding the composite system: Add 20g of liposome-nanoemulsion composite system and gently stir with a silicone spatula until there is no layering.

[0062] (5) Add essential oils and Tween-20: Add 2g of sweet orange essential oil, because sweet orange essential oil evaporates quickly. Add 1g of other essential oils. The essential oils will enhance the fragrance after being added to the soap. Then add 70mL of Tween-20 while stirring until you can draw an "∞" shape.

[0063] (6) Pour the product obtained in (5) into a mold, gently shake to remove air bubbles, and unmold after cooling to obtain the finished handmade soap. See product image below. Figure 1 .

[0064] Example 2

[0065] Weigh 60g of olive oil as the base oil, add 6g of the herbal compound extract and 0.5g of olive leaf extract, stir for 40 minutes to fully mix; add 0.5mL of sodium hydroxide solution to adjust the pH to 8, stir thoroughly, add 10g of the liposome-nanoemulsion composite system, and gently fold with a silicone spatula until no layering occurs, add 2g of sweet orange essential oil, stir to fully mix, add 50mL of Tween-20, see product image. Figure 2 .

[0066] Example 3

[0067] Weigh 80g of olive oil as the base oil, add 9g of herbal compound extract and 1g of olive leaf extract, stir for 40 minutes to mix thoroughly; add 2mL of sodium hydroxide solution to bring the pH to 8, stir thoroughly, add 30g of liposome-nanoemulsion composite system, gently fold with a silicone spatula until no layering occurs, add 3g of sweet orange essential oil, stir to mix thoroughly, add 60mL of Tween-20, see product image. Figure 3 .

[0068] Example 4

[0069] Weigh 100g of olive oil as the base oil, add 9g of herbal compound extract and 2g of olive leaf extract, stir for 40 minutes to mix thoroughly; add 5mL of sodium hydroxide solution to bring the pH to 8, stir thoroughly, add 50g of liposome-nanoemulsion composite system, gently fold with a silicone spatula until no layering occurs, add 3g of sweet orange essential oil, stir to mix thoroughly, add 70mL of Tween-20, see product image. Figure 4 .

[0070] Example 5: The remaining operations are the same as in Example 1, except that the compound extract of Chinese medicinal herbs is not added. See product image below. Figure 5 .

[0071] Example 6: The remaining operations are the same as in Example 1, except that olive leaf extract is not added. See product image below. Figure 6 .

[0072] Example 7: The remaining operations are the same as in Example 1, except that the liposome-nanoemulsion composite system is not added. See product image below. Figure 7 .

[0073] Comparative Example 1

[0074] Weigh 80g of olive oil as the base oil, add 20g of comfrey extract, 3g of compound Chinese herbal extract, and 5g of olive leaf extract, and stir for 40 minutes to mix thoroughly. Add 8mL of sodium hydroxide solution to bring the pH to 8, and stir thoroughly. Add 5g of liposome-nanoemulsion composite system, stir gently, add 6g of osmanthus essential oil, and stir to mix thoroughly. Add 70mL of Tween-20. (See product image) Figure 8 .

[0075] Comparative Example 2

[0076] Weigh 80g of olive oil as the base oil, add 20g of compound extract of traditional Chinese medicine and 1g of olive leaf extract, stir for 40 minutes to mix thoroughly; add 0.5mL of sodium hydroxide solution to adjust the pH to 8, stir thoroughly, add 2g of sweet orange essential oil, stir to mix thoroughly, add 70mL of Tween-20, see product image. Figure 9 .

[0077] Comparative Example 3

[0078] Weigh 80g of olive oil as the base oil, add 20g of comfrey extract, and stir for 40 minutes to mix thoroughly. Add 6mL of sodium hydroxide solution to bring the pH to 8, and stir thoroughly. Add 2g of osmanthus essential oil, and stir to mix thoroughly. Add 50mL of Tween-20. (See product image) Figure 10 .

[0079] Comparative Example 4: The remaining operations are the same as in Example 1, except that in step (5), Tween-20 is replaced with milk, and 25 mL is added. See the product image below. Figure 11 .

[0080] Comparative Example 5: The remaining operations were the same as in Example 1, except that the compound extract of traditional Chinese medicine, olive leaf extract, and liposome-nanoemulsion composite system were not added. See product image below. Figure 12 .

[0081] Comparative Example 6: The remaining operations are the same as in Example 1, except that Tween-20 is not added. See product image below. Figure 13 .

[0082] Quality evaluation tests were conducted on the above embodiments and comparative examples:

[0083] 1. pH: The pH values ​​of all products were tested according to GB / T 13531.1-2008 General Test Methods for Cosmetics, and are shown in Table 1 below.

[0084] Table 1 pH values ​​of different products

[0085] Product Number pH value Example 1 8.5 Example 2 8.6 Example 3 7.8 Example 4 8.9 Example 5 9.5 Example 6 7.8 Example 7 10.0 Comparative Example 1 8.2 Comparative Example 2 8.8 Comparative Example 3 8.7 Comparative Example 4 8.1 Comparative Example 5 7.5 Comparative Example 6 8.2

[0086] Handmade soaps can be used with a pH value below 10. If the pH drops to 8-9 (slightly alkaline), the effect is even better. The lower the pH, the better the effect, making it gentle and non-irritating, and more suitable for sensitive skin. When the pH of natural handmade soap reaches 7, it essentially loses its cleansing ability.

[0087] 2. Appearance, odor, total active ingredient content, moisture and volatile matter, total free alkali, chloride

[0088] Referring to GB 5009.236-2016 National Food Safety Standard for Determination of Moisture and Volatile Matter in Animal and Vegetable Oils, QB / T 2623.2-2020 Soap Test Methods: Determination of Total Free Alkali Content in Soap, and QB / T2623.6-2022 Soap Test Methods: Determination of Chloride Content in Soap, the above-mentioned examples and comparative examples were tested for the above-mentioned items. The final results all meet the relevant industry standards, as detailed below:

[0089] Table 2 Physicochemical properties of different products

[0090] project index Measured value Soap body appearance The soap is neatly arranged, with a uniform color, and free of obvious impurities and stains. conform to odor It has a stable aroma and no unpleasant odors such as rancidity. conform to Total active ingredient content, % ≥53 ≥70 Moisture and volatiles, % ≤30 ≤15％ Total free base (as NaOH), % ≤0.30 ≤0.1％ Free caustic alkali (calculated as NaOH), % ≤0.10 Not detected Chloride (as NaCl), % ≤1.0 ≤1.0

[0091] 3. Stability

[0092] Stability testing is an important step in assessing whether handmade soaps will deteriorate or lose performance after long-term storage. Test methods typically include measuring the molding and demolding time, drying, constant weight measurement, placing the handmade soap under specific conditions (such as room temperature, protection from light, and drying), and observing changes in appearance, odor, and texture over three time periods: six months, one year, and two years.

[0093] Observe the handmade soaps that have undergone stability testing for any cracking, deformation, oil seepage, or blooming. Also, check for any abnormal discoloration after two days of UV exposure. See Table 3 for specific results. If there are no obvious deformations, cracks, dents, oil spots, or odors, and the soap maintains its original hardness and solubility, it can be considered to have good stability. This indicates that the handmade soap can maintain its original quality and performance during storage, making it suitable for long-term use and preservation.

[0094] Table 3 Stability Tests for Different Products

[0095] Product Number Molding and demolding time / day stability Example 1 2 good Example 2 1 good Example 3 1 good Example 4 2 good Example 5 1 good Example 6 3 good Example 7 4 good Comparative Example 1 15 / Comparative Example 2 1 good Comparative Example 3 - / Comparative Example 4 - / Comparative Example 5 - / Comparative Example 6 - /

[0096] Note: "-" indicates that demolding has not been successful after more than 30 days, and " / " indicates poor stability or inability to test.

[0097] 4. Determination of mite-killing effect

[0098] Mite-repellent tests were conducted according to GB / T 24253-2009 Evaluation of Anti-mite Performance of Textiles and T / CASME 365-2023 Anti-mite Bathing Agents. A certain amount of handmade soap samples were dissolved in an appropriate amount of water (for products that could not be demolded, the semi-solid soap solution in the mold was used directly) to prepare soap solutions of different concentrations. A glass slide was placed in a shallow dish, and an appropriate amount of soap solution was added. Live mites were placed on the glass slide, ensuring they were in contact with the soap solution. The behavior and mortality of the mites were observed, and the corresponding observation results were recorded. The mite mortality rate in the sample solution for each handmade soap case was calculated. Furthermore, the mite-repellent effects of different case samples were compared to evaluate their anti-mite performance. See Table 4 for the mite-repellent effect test results.

[0099] Table 4 Results of mite removal efficacy test

[0100]

[0101] 5. Stain removal ability test

[0102] The products from the above examples and comparative examples were dissolved in water to prepare a 2 g / L test solution. Test pieces coated with equal amounts of oil and soil were immersed in the test solution at 30°C for 3 minutes, and then rinsed by a swishing motion, maintaining a swishing distance of approximately 50 mm and a swishing speed of 150 times / min. After rinsing, the samples were quickly removed, dried with hot air, cooled, and weighed.

[0103] Calculation formula:

[0104]

[0105] In the formula: m0 represents the mass of the test piece in g; m1 represents the mass of the test piece after being coated with oil in g; m2 represents the mass of the test piece after being cleaned of the oil-coated sample in g.

[0106] Table 5. Results of Decontamination Ability Test

[0107] Product Number Stain removal efficiency % Remark Example 1 99 Best effect Example 2 96 Good effect Example 3 95 Good effect Example 4 90 Good effect Example 5 50 Poor results Example 6 65 Poor results Example 7 60 Poor results Comparative Example 1 70 Unsatisfactory results Comparative Example 2 94 Good effect Comparative Example 3 96 Good effect Comparative Example 4 88 Good effect Comparative Example 5 70 Unsatisfactory results Comparative Example 6 76 Unsatisfactory results

[0108] II. Finished Product Testing and Inspection

[0109] 1. Skin feel was tested for gentleness, moisturizing effect, and hydrating performance.

[0110] Thirty healthy subjects aged 18-45 years were selected to test the mildness, moisturizing, and hydrating properties of Example 1. Volunteers rated the product, with 10 points indicating excellent mildness, moisturizing, and hydrating properties, 8 points indicating good product effect, 6 points indicating poor product effect, and so on.

[0111] Mildness test: Apply a small amount of handmade soap to the back of your hand or the inside of your wrist and observe for redness, itching, or irritation. If any adverse reaction occurs, it may indicate that the handmade soap contains irritating substances.

[0112] Moisturizing test: After washing hands with the handmade soap, observe the skin's moisture level. High-quality handmade soap should keep the skin soft and moisturized, avoiding dryness and tightness.

[0113] Moisturizing Performance Test: The moisturizing and repairing properties of the handmade soap were determined according to the "Test Method for Transepidermal Water Loss" (T / ZHCA 003-2018) standard for cosmetics, with TEWL (transepidermal water loss) value used as the transepidermal water loss value. The handmade medicated soap was applied evenly, and the subjects' hands were cleaned with water and patted dry with lint-free absorbent paper. The subjects sat still for 20 minutes in an environment with normal temperature, no significant air movement, and a relative humidity of 40%–60%. The left side was left as a blank. After 30 minutes, the TEWL values ​​of both hands were measured, and the rate of change was calculated using the following formula. A larger TEWL rate of change indicates a greater impact of the applied sample on the skin's moisturizing performance, meaning a better moisturizing and repairing effect of the medicated soap.

[0114]

[0115] T0 (TEWL value) represents the average value of the TEWL measurements from both hands;

[0116] T n (TEWL value) represents the average TEWL measurement values ​​of both hands at different measurement time points.

[0117] Table 6 shows the test results of mildness, moisturizing and hydrating properties in Example 1.

[0118] Volunteer ID mildness Moisturizing Moisturizing No. 1 10 10 10 No. 2 10 10 10 No. 3 10 10 8 No. 4 10 10 10 No. 5 10 10 10 No. 6 10 10 10 No. 7 10 10 10 No. 8 10 10 10 No. 9 10 10 10 No. 10 10 10 10 No. 11 10 10 10 No. 12 10 10 10 No. 13 10 10 8 No. 14 10 10 10 No. 15 10 10 10 No. 16 10 10 10 No. 17 10 10 10 No. 18 10 10 10 No. 19 10 10 10 No. 20 10 10 8 No. 21 10 10 10 No. 22 10 10 10 No. 23 10 10 10 No. 24 10 10 10 No. 25 10 10 10 No. 26 10 10 10 No. 27 10 10 10 No. 28 10 10 10 No. 29 10 10 10 No. 30 10 10 10

[0119] 2. Antibacterial activity

[0120] The antibacterial effect of all handmade soaps prepared in Example 1 was tested. Six samples were tested, and the antibacterial rate was greater than 50%, which meets the requirements of "GB 38456-2020 Hygienic Requirements for Antibacterial and Antimicrobial Detergents".

[0121] 3. Hyaluronidase inhibition rate experiment

[0122] Hyaluronic acid plays a major role in the development and regulation of skin moisture and elasticity, wound healing, and angiogenesis. It also participates in vasodilation and allergic reactions, thus being closely related to skin sensitivity. Inhibiting hyaluronidase activity prevents the breakdown of hyaluronic acid, thereby maintaining normal physiological functions. Anti-allergic activity is indicated by the hyaluronidase inhibition rate; a higher inhibition rate indicates stronger anti-allergic activity.

[0123] (1) Enzyme activity assay: Add appropriate amounts of hyaluronidase and sodium hyaluronate, along with a buffer solution, to a test tube. Incubate at 37°C for a period of time to allow the enzyme to fully react. Add a colorimetric reagent and measure the absorbance at a specific wavelength, recording the result as the control value.

[0124] (2) Inhibitor test: Dilute the handmade soap sample to be tested to an appropriate concentration. Add the same reagent as the control group to another set of test tubes, but add the handmade soap sample as an inhibitor. Incubate at a certain temperature and measure the absorbance, recording it as the sample value.

[0125] During the experiment, observe for any abnormal phenomena, such as color changes or precipitation, and record these phenomena.

[0126] Hyaluronidase inhibition rate (%) = (control value - sample value) / control value × 100%.

[0127] According to the test results, the hyaluronic acid inhibition rate of Example 1 is greater than 50%, and the handmade soap has anti-allergic activity.

[0128] 4. Skin allergy test:

[0129] Test method: 1. Select a small area of ​​skin on the inner arm and behind the ear that are sensitive but not easily exposed to direct sunlight for a small-area skin test. Apply a small amount of the handmade soap from Example 1 to the test area and wait for a period of time (e.g., 24 hours) to observe whether there are any allergic reactions such as redness, swelling, itching, or stinging. 2. Use the product from Example 1 to cleanse every morning, noon and evening. After using it continuously for one month, carefully observe the cleaned area and record the changes.

[0130] Test participants: 30 young female volunteers aged 18 to 40 were selected for the test.

[0131] Controlled trial: Select a soap-free product suitable for sensitive skin as a control and use it alternately with the handmade soap to assess the differences in symptoms and determine whether the handmade soap will cause an allergic reaction.

[0132] Table 7 Skin Allergy Test

[0133]

[0134]

[0135] 5. Referring to GB / T 39665-2020, "Determination of Residues of 55 Banned Pesticides in Cosmetics Containing Plant Extracts", liquid chromatography-mass spectrometry (LC-MS) was used to detect residues of 120 pesticides.

[0136] The pesticide residue detection in Example 1 was performed as follows: 1g of sample was scraped into a 50mL stoppered centrifuge tube, 10mL of acetonitrile was added, and the mixture was vortexed for 1min. Then, it was sonicated for 10min, followed by the addition of 5g of sodium chloride. After vortexing for 1min, the mixture was centrifuged at 8000r / min for 2min. The supernatant was transferred to a new 50mL stoppered centrifuge tube. The residue was extracted again with 10mL of acetonitrile. The supernatants were combined in the same centrifuge tube and concentrated to near dryness using a nitrogen concentrator at 40℃. Approximately 10mL of ethyl acetate solution was added to the centrifuge tube to dissolve the residue. The solution was then transferred to a 10mL volumetric flask and brought to volume. The solution was filtered through an organic phase filter membrane into a gel permeation chromatograph vial. The sample was collected in a gel permeation chromatograph collection bottle, and the collected solution was transferred to a 250 mL flat-bottom flask. The collection bottle was washed twice with 10 mL of ethyl acetate solution, and the solutions were combined and transferred to the flat-bottom flask. The solution was evaporated to near dryness using a rotary evaporator at a water bath temperature of 40 °C. 0.5 mL of acetonitrile solution was added to dissolve the residue, and the mixture was mixed well. The solution was then filtered through an organic phase filter membrane (0.22 μm) for determination by liquid chromatography-tandem mass spectrometry. Testing revealed that none of the above products tested positive for any of the 80 prohibited pesticides (the pesticides tested were: carbendazim, thiamethoxam, dicofol, diflubenzuron, flutriafol, dicofol, quizalofop-P-ethyl, fluoxazin, iodobenzonitrile, isopropylidene diketone, isoxaflutole, fenoxyfen, linuron, quizalofop-P-ethyl, chlorpyrifos, propargite, propargite, pyridaben, simazine, and tridemorph, totaling 80 pesticides).

[0137] Figure 14 The chromatograms are for 80 mixed standard samples at 5 μg / kg and for the sample from Example 1. No pesticide residues were detected in the product from Example 1.

Claims

1. A pure natural antibacterial and anti-allergic olive oil handmade soap, characterized in that, The handmade soap is made from the following ingredients in parts by weight: The mixture consists of 10-50 parts of a liposome-nanoemulsion complex system, 60-100 parts of olive oil, 0.5-5 parts of alkali solution, 0.5-2 parts of olive leaf extract, 6-9 parts of a complex Chinese herbal medicine extract, 2-3 parts of essential oil, and 50-70 parts of Tween-20.

2. The all-natural antibacterial and anti-allergic olive oil handmade soap as described in claim 1, characterized in that, The alkaline solution is a sodium hydroxide solution with a concentration of 0.2–0.6 g / mL.

3. The all-natural antibacterial and anti-allergic olive oil handmade soap as described in claim 1, characterized in that, The preparation method of the liposome-nanoemulsion composite system is as follows: The liposome suspension and nanoemulsion were mixed at a volume ratio of 1:1 to 5 and stirred at 200 rpm for 15 to 45 minutes to form a composite system.

4. The all-natural antibacterial and anti-allergic olive oil handmade soap as described in claim 3, characterized in that, The liposome suspension is prepared as follows: (1) Film formation: Dissolve 0.3g soybean lecithin, 0.1g cholesterol and 0.1g oat alkaloids in 10mL chloroform and evaporate at 50℃ for 10min to form a homogeneous film; (2) Hydration: 15 mL of pH 7.0 phosphate buffer was added to the homogenized film in step (1), and after hydration, it was ultrasonically treated to control the particle size to 100-200 nm. (3) Purification: High-speed centrifugation was used to remove unencapsulated components to prepare the liposome suspension.

5. The all-natural antibacterial and anti-allergic olive oil handmade soap as described in claim 4, characterized in that, The preparation method of the nanoemulsion is as follows: (1) Primary emulsification: Mix 5g of olive oil with 1g of emulsifier (Tween-20), slowly add 40mL of water, and stir at 1000rpm for 10min to form a coarse emulsion; (2) High-pressure homogenization: Use a homogenizer to circulate the liquid until the droplet size is <200nm; (3) Stability treatment: Add 0.06g of 1% vitamin E and store at 4℃.

6. The all-natural antibacterial and anti-allergic olive oil handmade soap as described in claim 1, characterized in that, The essential oil is 2-3 parts sweet orange essential oil or 1-2 parts osmanthus essential oil.

7. The all-natural antibacterial and anti-allergic olive oil handmade soap as described in claim 1, characterized in that, The compound Chinese herbal extract is obtained by mixing extracts of Saposhnikovia divaricata, Sophora flavescens, Scutellaria baicalensis, Kochia scoparia, Dictamnus dasycarpus, and Glycyrrhiza uralensis, adding isooctyl palmitate at a mass-volume ratio of 1:10, and stirring thoroughly.

8. The all-natural antibacterial and anti-allergic olive oil handmade soap as described in claim 1, characterized in that, The Saposhnikovia extract is obtained by pulverizing Saposhnikovia divaricata, weighing 5g, adding 200mL of 95% ethanol solution, ultrasonically extracting at 50℃ for 30min, filtering, and then reducing the pressure, concentrating, and drying the filtrate. The Sophora flavescens extract is prepared by crushing Sophora flavescens through a 0.2mm sieve, weighing 10g of powder, adding 55% ethanol at a material-to-liquid mass-to-volume ratio of 1:10, refluxing at 80℃ for 1h, extracting twice, combining the two filtrates, concentrating and drying. The Scutellaria baicalensis extract is prepared by pulverizing Scutellaria baicalensis through a 0.2 mm sieve, weighing 10 g of powder, adding 70% ethanol at a material-to-liquid mass-to-volume ratio of 1:10, refluxing at 80°C for 2 h, extracting twice, combining the two filtrates, and then rotary evaporating and concentrating the filtrate at 55°C. The Kochia scoparia extract is obtained by pulverizing Kochia scoparia, weighing 5g, adding 50mL of 45% ethanol solution, extracting in a constant temperature water bath at 70℃ for 5h, filtering, and then reducing the pressure, concentrating and drying the filtrate. The extract of Dictamnus dasycarpus root bark is prepared by crushing Dictamnus dasycarpus root bark, weighing 20g, placing it in a 250mL round-bottom flask, adding 100mL of petroleum ether and soaking for 30min, refluxing and extracting three times, each time for 2h, filtering and combining the extracts, and then concentrating and drying under reduced pressure. The licorice extract is obtained by pulverizing licorice, weighing 5g, adding 50mL of 75% ethanol solution, ultrasonically treating for 30min, covering and soaking at room temperature for 1 week, filtering, and then reducing the pressure, concentrating and drying the filtrate.

9. A method for preparing a pure natural antibacterial and anti-allergic olive oil handmade soap as described in any one of claims 1-8, characterized in that, The preparation method includes the following steps: (1) Dissolving alkali: Weigh the alkali substance, add deionized water, stir to dissolve, and wait for the temperature to drop to 38℃ to obtain the alkali solution; (2) Oil preparation: Olive oil is mixed with compound Chinese herbal extracts, olive leaf extract is added, and the mixture is stirred until well mixed to obtain oil; (3) Soap base preparation: Mix the alkaline solution obtained in (1) and the oil obtained in (2), stir, and obtain soap base; (4) Adding the composite system: Slowly pour the liposome-nanoemulsion composite system into the soap base and gently stir until it is evenly dispersed without layering. (5) Add essential oil and Tween-20: Add essential oil to the product obtained in (4), continue stirring, then add Tween-20 and continue stirring until Trace state; (6) Molding and solidification: Pour the product obtained in (5) into the mold, gently shake it to remove air bubbles, and demold it after cooling to obtain the finished handmade soap.

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