Microalgae and methanotrophic bacteria isolated culture device

Abstract

The invention relates to the technical field of agricultural waste high-value utilization, in particular to a microalgae and methanotrophic bacteria isolated culture device.The microalgae and methanotrophic bacteria isolated culture device comprises a first culture chamber, a second culture chamber, a semi-permeable membrane and a culture medium conveying mechanism, the first culture chamber is used for culturing microalgae, and the second culture chamber is used for culturing methanotrophic bacteria; the first culture chamber is provided with a first discharge hole; the second culture chamber and the first culture chamber form a nested structure, the second culture chamber is used for culturing methane nutrition bacteria, and the second culture chamber is provided with a second discharge port; the semi-permeable membrane is arranged between the first culture chamber and the second culture chamber and is used for separating the microalgae from the methanotrophic bacteria and allowing gas and liquid to circulate; the culture medium conveying mechanism is used for introducing biogas slurry and biogas into the first culture chamber and the second culture chamber, and the microalgae and methane nutrient bacterium separation culture device provided by the embodiment of the invention can realize effective exchange and cyclic utilization of substances while ensuring that two microorganisms obtain respective suitable growth environments.

Description

Technical Field

[0001] This invention relates to the field of high-value utilization technology of agricultural waste, and in particular to a device for separating and cultivating microalgae and methanogenic bacteria. Background Technology

[0002] With increasingly stringent environmental protection requirements, technologies utilizing microorganisms to treat organic wastewater are being widely applied. Among these, microalgae and methanogenic bacteria cultivation systems hold significant value in wastewater treatment. Microalgae can absorb carbon dioxide and release oxygen through photosynthesis, while simultaneously producing economically valuable biomass; methanogenic bacteria, on the other hand, can utilize oxygen and methane to produce single-cell proteins, thereby reducing greenhouse gas emissions.

[0003] Currently, the cultivation of microalgae and methanogenic bacteria mainly employs two methods: one is to co-culture the two microorganisms in the same culture chamber, and the other is to cultivate them independently using separate culture devices. While co-culture is structurally simple, it is not conducive to subsequent biomass separation, and the two microorganisms compete for nutrients, resulting in lower quality biomass products. Independent cultivation, on the other hand, provides optimal growth environments for each microorganism, but requires separate culture devices, increasing equipment investment and preventing material exchange and recycling between the two microorganisms, leading to resource waste. Summary of the Invention

[0004] This invention provides a device for separating and culturing microalgae and methanogenic bacteria. This device ensures complete separation of the two microbial products while also enabling effective exchange and recycling of substances.

[0005] This invention provides a device for separating and culturing microalgae and methanogenic bacteria, comprising: a first culture chamber for culturing microalgae, the first culture chamber having a first outlet; a second culture chamber, forming a nested structure with the first culture chamber, the second culture chamber for culturing methanogenic bacteria, the second culture chamber having a second outlet; a semi-permeable membrane disposed between the first culture chamber and the second culture chamber for separating microalgae and methanogenic bacteria while allowing gas and liquid to flow; and a culture medium conveying mechanism for introducing biogas slurry and biogas into the first culture chamber and the second culture chamber.

[0006] In one possible implementation, the second culture chamber is nested within the first culture chamber, and the output end of the culture medium delivery mechanism is connected to the first culture chamber.

[0007] In one possible implementation, the second culture chamber is equipped with a first stirring assembly.

[0008] In one possible implementation, a first aeration component is also included, the input end of which is connected to the top of the second culture chamber, and the first aeration plate of the first aeration component is located at the bottom of the second culture chamber.

[0009] In one possible implementation, a lighting assembly is also included, which is disposed on the inner wall of the first culture chamber.

[0010] In one possible implementation, the culture medium delivery mechanism includes: an anaerobic fermentation chamber with a feed inlet, a first heating component and a second stirring component installed inside the anaerobic fermentation chamber; a pretreatment chamber with a third stirring component installed inside the pretreatment chamber, an adjustment port connected to the top of the pretreatment chamber, and a first acid-base monitoring probe installed inside the pretreatment chamber; a biogas delivery unit, with its input end connected to the anaerobic fermentation chamber and its output end connected to the first culture chamber; a first liquid delivery unit, with its input end connected to the supernatant in the anaerobic fermentation chamber and its output end connected to the pretreatment chamber; and a second liquid delivery unit, with its input end located at the bottom of the pretreatment chamber and its output end connected to the first culture chamber.

[0011] In one possible implementation, the biogas delivery unit includes: a gas storage device; a gas pump, the input end of which is connected to the anaerobic fermentation chamber and the output end of which is connected to the gas storage device; and a second aeration assembly, the input end of which is connected to the gas storage device, and the second aeration plate of the second aeration assembly is located at the bottom of the first culture chamber.

[0012] In one possible implementation, the biogas delivery unit further includes a gas flow rate detection device, which is installed in the pipeline of the second aeration component to control the aeration rate of the second aeration component.

[0013] In one possible implementation, the first liquid delivery unit includes: a delivery pipeline, with its two ends connected to the anaerobic fermentation chamber and the pretreatment chamber, respectively; a pump body disposed on the delivery pipeline for delivering the liquid in the delivery pipeline; and a valve disposed in the delivery pipeline for controlling the opening degree of the delivery pipeline.

[0014] In one possible implementation, the first liquid delivery unit further includes a filter membrane disposed in the liquid delivery line.

[0015] The microalgae and methanogenic bacteria separation and cultivation device provided by this invention achieves the separate cultivation of microalgae and methanogenic bacteria through a nested structure design of a first and second cultivation chamber, with a semi-permeable membrane placed between the two chambers. The semi-permeable membrane has a specific pore size that prevents the penetration of microbial cells but allows gas molecules and soluble substances to pass through. Therefore, oxygen produced by microalgae during photosynthesis in the first cultivation chamber can pass through the semi-permeable membrane into the second cultivation chamber, promoting the growth of methanogenic bacteria. Simultaneously, carbon dioxide produced by the metabolism of methanogenic bacteria in the second cultivation chamber can diffuse through the semi-permeable membrane to the first cultivation chamber and be utilized by the microalgae. This material exchange process is based on the principle of molecular diffusion and requires no additional energy input. By providing outlets in the first and second cultivation chambers respectively, the culture products of the two microorganisms can be conveniently collected separately. The culture medium delivery mechanism ensures a continuous supply of the culture medium required for the cultivation process. These structural designs enable the entire simultaneous cultivation device to maintain stable operation. In summary, the microalgae and methanogenic bacteria separation and cultivation device provided by this invention solves the technical problem of the difficulty in separating microalgae and methanogenic bacteria in mixed cultivation, allowing both microorganisms to obtain a suitable growth environment. On the other hand, it maintains the material exchange function between the two microorganisms, realizes the recycling of the cultivation medium, and overcomes the resource waste problem of independent cultivation methods. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the planar structure of a microalgae and methanogenic bacteria separation and culture device provided by the present invention.

[0018] Figure 2 This is a three-dimensional perspective structural diagram of a first culture chamber and a second culture chamber provided in an embodiment of the present invention.

[0019] Figure 3 This is a top view schematic diagram of a device for separating and culturing microalgae and methanogenic bacteria according to an embodiment of the present invention.

[0020] Figure 4 This is a partially enlarged structural schematic diagram of a first liquid delivery unit provided in an embodiment of the present invention.

[0021] in: 1. First incubation chamber; 11. First discharge port; 12. Lighting assembly; 13. First feeding port; 2. Second culture chamber; 21. Second discharge port; 22. First stirring assembly; 23. Dissolved oxygen probe; 24. Second acid-base monitoring probe; 25. Second feed port; 3. Semi-permeable membrane; 4. First aeration component; 41. First aeration plate; 5. Anaerobic fermentation chamber; 51. Feed inlet; 52. First heating component; 53. Second stirring component; 6. Pretreatment chamber; 61. Third stirring assembly; 62. Water inlet; 63. pH adjustment port; 64. First acid-base monitoring probe; 7. Biogas delivery unit; 71. Gas storage device; 72. Air pump; 73. Second aeration assembly; 731. Second aeration plate; 8. First liquid delivery unit; 81. Infusion pipeline; 82. Pump body; 83. Valve; 84. Filter membrane; 9. Second liquid delivery unit. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0023] The following is combined with Figure 1-4 This invention describes a device for separating and culturing microalgae and methanogenic bacteria, comprising: a first culture chamber 1, a second culture chamber 2, a semi-permeable membrane 3, and a culture medium delivery mechanism, wherein: The first culture chamber 1 is used to cultivate microalgae, and the first culture chamber 1 has a first discharge port 11. The first discharge port 11 is used to discharge microalgal protein.

[0024] The second culture chamber 2 forms a nested structure with the first culture chamber 1. The second culture chamber 2 is used to cultivate methanogenic bacteria and has a second discharge port 21. The first culture chamber 1 can be nested inside or outside the second culture chamber 2. The second discharge port 21 is used to discharge bacterial protein.

[0025] A semi-permeable membrane 3 is disposed between the first culture chamber 1 and the second culture chamber 2 to separate microalgae and methanogenic bacteria while allowing gas and liquid to flow.

[0026] The culture medium conveying mechanism is used to introduce biogas slurry and biogas into the first culture chamber 1 and the second culture chamber 2.

[0027] In this invention, the first culture chamber 1 and the second culture chamber 2 adopt a nested structure design. Compared with the traditional side-by-side placement, this design saves equipment floor space and avoids the light attenuation problem inside traditional microalgae culture chambers by using the outer culture chamber for cultivating microalgae. The semi-permeable membrane 3, through its specific pore size and material properties, can separate the two types of microorganisms to avoid mutual interference while ensuring normal exchange of gases and soluble substances. This design allows methane in biogas to be effectively utilized by methanogenic bacteria, while the carbon dioxide produced by biogas and methanogenic bacteria metabolism can be utilized by microalgae, forming a material recycling process. In addition, the first culture chamber 1 and the second culture chamber 2 are respectively equipped with a first outlet 11 and a second outlet 21 to facilitate the collection and separation of culture products. The culture medium delivery mechanism ensures a continuous supply of nutrients to the entire system, enabling the system to maintain stable operation.

[0028] Furthermore, the nested structure design improves space utilization efficiency. Compared to the parallel placement method in the prior art, the synchronous culture device of the present invention, through its inner and outer two-layer arrangement, can achieve a larger culture volume under the same floor space conditions. Simultaneously, the culture medium in the first culture chamber 1 can serve as an insulation medium for the second culture chamber 2, reducing heat loss.

[0029] Specifically, the semi-permeable membrane 3 uses a 0.2-micron nanofiltration membrane. The nanofiltration membrane is only used to isolate microalgae and methanogenic bacteria, while gas and liquid can flow between the two chambers. The nested structure of the two chambers separates the algae and bacteria for cultivation, avoiding the self-shading problem of mixed cultivation of microalgae and methanogenic bacteria, increasing the light-receiving area of ​​the microalgae, and allowing the two products to be collected separately, thereby improving the quality and yield of microbial proteins.

[0030] The second culture chamber 2 is equipped with a dissolved oxygen probe 23 and a second acid-base monitoring probe 24 to monitor the solution conditions in real time. The top of the first culture chamber 1 is equipped with a first feed port 13 for adding microalgae; the top of the second culture chamber 2 is equipped with a second feed port 25 for adding methanogenic bacteria.

[0031] In some embodiments, the second culture chamber 2 is nested within the first culture chamber 1, and the output end of the culture medium delivery mechanism is connected to the first culture chamber 1.

[0032] This invention specifically defines a structural feature where the second culture chamber 2 is nested within the first culture chamber 1. This nested arrangement, because microalgae cultivation requires light and generates heat during the process, can maintain the temperature of the inner methanogenic bacteria culture chamber through heat conduction, reducing additional heating energy consumption. Secondly, the design of the culture medium delivery mechanism being directly connected to the first culture chamber 1 simplifies the piping system, reduces pipe connection points, and lowers the risk of leakage. Thirdly, this structural design facilitates system cleaning and maintenance, reducing the creation of dead zones. In practical applications, when the system requires cleaning, the inner second culture chamber 2 can be easily disassembled for separate cleaning.

[0033] Specifically, a water bath heating component is installed on the outside of the first culture chamber 1, which can provide a suitable temperature environment for the first culture chamber 1.

[0034] In some embodiments, a first stirring assembly 22 is provided in the second culture chamber 2.

[0035] In this embodiment of the invention, a first stirring component 22 is added to the second culture chamber 2. The stirring by the first stirring component 22 prevents the sedimentation of methanogenic bacteria cells during cultivation, maintaining the homogeneity of the culture medium. The stirring process breaks up larger air bubbles, forming more smaller bubbles, increasing the gas-liquid contact area and improving gas mass transfer efficiency. This is crucial for improving the utilization rate of gases such as methane. Experiments have shown that an appropriate stirring speed can increase gas utilization by approximately 15%. Simultaneously, stirring also promotes the uniform distribution of nutrients in the culture medium, providing better environmental conditions for the growth of methanogenic bacteria.

[0036] In some embodiments, a first aeration component 4 is also included, the input end of which is connected to the top of the second culture chamber 2, and the first aeration plate 41 of the first aeration component 4 is located at the bottom of the second culture chamber 2.

[0037] In this invention, the input end of the first aeration component 4 is connected to the top of the second culture chamber 2, while the first aeration plate 41 is located at the bottom, forming a gas recycling pathway. When the gas rises to the top of the second culture chamber 2, any unused gas can be collected and reused, improving gas utilization efficiency. Bottom aeration causes the bubbles to move upwards, providing both aeration and a certain degree of stirring. This arrangement prolongs the residence time of the gas in the culture medium, ensuring sufficient contact between the gas and the culture medium, and improving mass transfer efficiency.

[0038] Specifically, the first aeration component 4 is also equipped with a gas flow rate detection unit, which controls the aeration rate of the first aeration component 4 by detecting the gas flow rate.

[0039] In some embodiments, an illumination component 12 is also included, which is disposed on the inner sidewall of the first culture chamber 1.

[0040] In this invention, the lighting component 12 is positioned on the inner wall of the first culture chamber 1. This arrangement brings the light source closer to the culture medium, reducing light energy loss during transmission. The annular arrangement on the inner wall ensures uniform illumination and avoids blind spots. From a maintenance perspective, installation on the inner wall facilitates inspection and replacement of the lighting fixtures. In practical applications, this arrangement provides stable lighting conditions for microalgae, ensuring their normal growth and photosynthesis.

[0041] Specifically, the first culture chamber 1 has a rectangular structure, and strip LED lights are installed on the four inner walls of the first culture chamber 1, arranged at 2 cm intervals from above the aeration plate to a height of 26 cm, to ensure that sufficient light can be provided for the microalgae in the first culture chamber 1.

[0042] In some embodiments, the culture medium conveying mechanism includes: an anaerobic fermentation chamber 5 having an inlet 51, a first heating component 52 disposed inside the anaerobic fermentation chamber 5, and a second stirring component 53 disposed inside the anaerobic fermentation chamber 5; a pretreatment chamber 6 having a third stirring component 61 disposed inside the pretreatment chamber 6, an adjustment port connected to the top of the pretreatment chamber 6, and a first acid-base monitoring probe 64 disposed inside the pretreatment chamber 6; a biogas conveying unit 7 having its input end connected to the anaerobic fermentation chamber 5 and its output end connected to the first culture chamber 1; a first liquid conveying unit 8 having its input end connected to the supernatant in the anaerobic fermentation chamber 5 and its output end connected to the pretreatment chamber 6; and a second liquid conveying unit 9 having its input end located at the bottom of the pretreatment chamber 6 and its output end connected to the first culture chamber 1.

[0043] This invention describes in detail the structure and working principle of the culture medium delivery mechanism. A first heating component 52 is installed in the anaerobic fermentation chamber 5 to maintain the temperature conditions required for fermentation. Specifically, when the ambient temperature is low, the first heating component 52 can maintain the fermentation chamber temperature within the mesophilic fermentation range of 35-37℃, ensuring stable fermentation. The pretreatment chamber 6 reflects the requirements for controlling the quality of the culture medium. The third stirring component 61 ensures that the regulators added during pretreatment are fully mixed. The adjustment port on the pretreatment chamber 6 facilitates the addition of substances such as pH regulators, and the first acid-base monitoring probe 64 can monitor pH changes in real time, providing a basis for adding regulators. The biogas delivery unit 7 and the two liquid delivery units form a complete material delivery system. Their coordination ensures the timely supply of substances required for the cultivation process. For example, when the dissolved oxygen content in the first culture chamber 1 decreases, the biogas delivery unit 7 can increase the aeration rate; when the nutrients in the culture medium are insufficient, the liquid delivery units can supplement with fresh culture medium.

[0044] Specifically, the first heating component 52 uses a water bath heating system, consisting of a water bath jacket 1 cm outside the outer wall of the anaerobic fermentation chamber 5, used to maintain the temperature inside the anaerobic fermentation chamber 5 at 35°C, allowing the material to undergo mesophilic fermentation. The first liquid conveying unit 8 transports the supernatant liquid from the anaerobic fermentation chamber 5 to the pretreatment chamber 6 for pretreatment. The input end of the second liquid conveying unit 9 extends into the bottom of the pretreatment chamber 6, transporting the fully treated biogas slurry from the bottom into the first culture chamber 1, ensuring sufficient pretreatment of the biogas slurry. The regulating ports include a water inlet 62 and a pH adjustment port 63. The ammonia nitrogen concentration of the biogas slurry produced by the anaerobic fermentation chamber 5 is approximately 2000 mg / L. The biogas slurry entering the pretreatment chamber 6 is diluted with water to an ammonia nitrogen concentration of approximately 500 mg / L, and the pH is adjusted to approximately 6.8~7.0. A spiral stirrer is used to ensure thorough mixing of the liquid.

[0045] The entire cultivation device uses a single shell, within which are partitions forming separate compartments: an anaerobic fermentation chamber 5, a pretreatment chamber 6, and cultivation chambers (first cultivation chamber 1 and second cultivation chamber 2). To improve the anaerobic fermentation effect, a second stirring assembly 53 is installed in the anaerobic fermentation chamber 5.

[0046] In some embodiments, the biogas delivery unit 7 includes: a gas storage device 71; a gas pump 72, the input end of which is connected to the anaerobic fermentation chamber 5 and the output end of which is connected to the gas storage device 71; and a second aeration assembly 73, the input end of which is connected to the gas storage device 71 and the second aeration plate 731 of the second aeration assembly 73 is located at the bottom of the first culture chamber 1.

[0047] This embodiment of the invention specifically defines the components of the biogas delivery unit 7. The gas storage device 71 solves the problem of uneven biogas production: when the anaerobic fermentation chamber 5 produces a large amount of biogas, the excess biogas can be temporarily stored in the gas storage device 71; when the production is insufficient, the biogas in the gas storage device 71 can be used to supplement it. This "peak-valley regulation" function ensures the continuous and stable operation of the system. The air pump 72 enables precise control of the gas delivery pressure; the gas delivery rate can be controlled by adjusting the speed of the air pump 72. The second aeration plate 731 of the second aeration component 73 is located at the bottom of the first culture chamber 1. This arrangement can generate uniform small bubbles, increasing the gas-liquid contact area. During the rising process of the small bubbles, due to the large specific surface area, the gas mass transfer effect is better. At the same time, the rising bubbles can also drive the culture liquid to circulate, playing a partial stirring role.

[0048] In some embodiments, the biogas delivery unit 7 further includes a gas flow rate detection device disposed in the pipeline of the second aeration component 73 for controlling the aeration rate of the second aeration component 73.

[0049] This invention incorporates a gas flow rate detection device. In practical applications, the control of gas flow rate directly affects the system's performance: too low a flow rate leads to insufficient gas supply, hindering microbial growth; too high a flow rate not only wastes energy but may also damage microbial cells due to excessive shear force. The gas flow rate detection device enables real-time monitoring and precise control of the aeration rate. For example, in the early stages of microalgae cultivation, a lower aeration rate can be used due to the small biomass; as the cultivation density increases, the aeration rate can be gradually increased, satisfying growth requirements while avoiding resource waste. This dynamic adjustment capability keeps the system in optimal operating condition at all times.

[0050] In some embodiments, the first liquid delivery unit 8 includes: a delivery pipeline 81, the two ends of which are respectively connected to the anaerobic fermentation chamber 5 and the pretreatment chamber 6; a pump body 82, disposed on the delivery pipeline 81, for delivering liquid in the delivery pipeline 81; and a valve 83, disposed in the delivery pipeline 81, for controlling the opening degree of the delivery pipeline 81.

[0051] This embodiment of the invention describes the specific structure of the first liquid delivery unit 8. The infusion pipeline 81 constructs the channel for liquid transmission; its arrangement must consider hydrodynamic characteristics to avoid dead zones and sedimentation. The pump body 82 provides the power for liquid delivery; its selection must consider factors such as delivery distance and head. The valve 83 enables precise flow control, allowing adjustment of the culture medium replenishment amount according to cultivation needs. The cooperation of these three components forms a complete liquid delivery system. In practical applications, this system can automatically adjust the culture medium replenishment amount according to changes in cultivation parameters. For example, when a deficiency of certain nutrients in the culture medium is detected, the system can automatically open valve 83 to replenish fresh culture medium.

[0052] In some embodiments, the first liquid delivery unit 8 further includes a filter membrane 84 disposed in the liquid delivery line 81.

[0053] In this invention, a filter membrane 84 is added to the first liquid delivery unit 8. This design plays a crucial role in ensuring the quality of the culture medium. In practical applications, anaerobic fermentation broth often contains suspended solids, impurities, and other substances that affect microbial growth. The filter membrane 84 can retain these substances, allowing only beneficial components to pass through. For example, when treating agricultural organic wastewater containing a large amount of solid particles, the filter membrane 84 can remove solid impurities, ensuring that clean culture medium is delivered to the culture system. This pretreatment not only protects the subsequent culture process but also extends the service life of the equipment.

[0054] Specifically, the filter membrane 84 uses a 0.45-micron nanofiltration membrane, which can trap harmful macromolecules.

[0055] Example: 1. Agricultural waste such as chicken manure and straw are pre-treated externally before being added to the anaerobic fermentation chamber for mesophilic fermentation. Once gas production stabilizes, a water pump transports the supernatant from the anaerobic fermentation chamber to the pretreatment chamber. The transport pipeline also functions as a filter, with a 0.45-micron nanofiltration membrane and a water pump in the middle to trap harmful macromolecules. Valves on both sides of the channel control the flow rate and speed of the liquid, allowing a suitable amount of biogas slurry to be drawn into the pretreatment chamber each time.

[0056] 2. The ammonia nitrogen concentration of the biogas slurry produced in the anaerobic fermentation chamber is approximately 2000 mg / L. The biogas slurry entering the pretreatment chamber is diluted with water to an ammonia nitrogen concentration of approximately 500 mg / L, and the pH is adjusted to approximately 6.8-7.0. A spiral agitator is used to ensure thorough mixing of the liquid. Subsequently, a water pump is used to transport the treated biogas slurry to the microalgae and methanogenic bacteria cultivation chamber. The transport pipeline also functions as a filter, with a 0.2-micron nanofiltration membrane in the middle capable of trapping harmful macromolecules. The biogas produced in the anaerobic fermentation chamber is collected in the gas storage chamber. The gas input rate is related to the volume of the cultivation chamber. Taking a 3L microalgae cultivation chamber and a 3L methanogenic bacteria chamber as an example, gas can be supplied to the microalgae cultivation chamber at an input rate of 1L / day.

[0057] 3. Biogas enters the microalgae cultivation chamber through bottom aeration plates. Carbon dioxide in the biogas is absorbed by the microalgae through photosynthesis, producing oxygen. Simultaneously, the microalgae utilize nutrients such as ammonia nitrogen and other small molecules in the biogas slurry for biomass accumulation. The methane and oxygen mixture accumulated in the headspace of the microalgae cultivation chamber is pumped into the methanogenic bacteria cultivation chamber at a rate of 300-500 mL / day for the growth of methanogenic bacteria and the production of microbial protein. A fed-batch culture method is used, meaning that the algae and bacteria generated in the cultivation chamber are collected daily, while biogas slurry from the pretreatment chamber is supplied daily as needed.

[0058] 4. The microalgae culture chamber and the methanogenic bacteria culture chamber are separated by a 0.2-micron nanofiltration membrane. The nanofiltration membrane is only used to isolate the microalgae and methanogenic bacteria, while gas and liquid can flow between the two chambers. The nested structure of the two chambers separates the algae and bacteria for cultivation, avoiding the self-shading problem of mixed cultivation of microalgae and methanogenic bacteria, increasing the light-receiving area of ​​the microalgae, and allowing the two products to be collected separately, thereby improving the quality and yield of microbial proteins.

[0059] The device embodiments described above are merely illustrative. The units described as separate components may or may not be physically separate, and the components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the modules can be selected to achieve the purpose of this embodiment according to actual needs. Those skilled in the art can understand and implement this without any creative effort.

[0060] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A device for separating and culturing microalgae and methanogenic bacteria, characterized in that, include: The first culture chamber (1) is used to cultivate microalgae, and the first culture chamber (1) has a first discharge port (11). The second culture chamber (2) forms a nested structure with the first culture chamber (1). The second culture chamber (2) is used to cultivate methanogenic bacteria. The second culture chamber (2) has a second discharge port (21). A semi-permeable membrane (3) is disposed between the first culture chamber (1) and the second culture chamber (2) to separate microalgae and methanogenic bacteria and allow gas and liquid to flow; The culture medium conveying mechanism is used to introduce biogas slurry and biogas into the first culture chamber (1) and the second culture chamber (2).

2. The microalgae and methanogenic bacteria separation and cultivation device according to claim 1, characterized in that, The second culture chamber (2) is nested inside the first culture chamber (1), and the output end of the culture medium delivery mechanism is connected to the first culture chamber (1).

3. The microalgae and methanogenic bacteria separation and cultivation device according to claim 2, characterized in that, The second culture chamber (2) is equipped with a first stirring assembly (22).

4. The microalgae and methanogenic bacteria separation and cultivation device according to claim 2, characterized in that, It also includes a first aeration component (4), the input end of which is connected to the top of the second culture chamber (2), and the first aeration plate (41) of the first aeration component (4) is located at the bottom of the second culture chamber (2).

5. The microalgae and methanogenic bacteria separation and cultivation device according to claim 2, characterized in that, It also includes a lighting assembly (12) disposed on the inner wall of the first culture chamber (1).

6. The microalgae and methanogenic bacteria separation and cultivation device according to any one of claims 2-5, characterized in that, The culture medium delivery mechanism includes: The anaerobic fermentation chamber (5) has a feed inlet (51), a first heating component (52) is provided on the outer wall of the anaerobic fermentation chamber (5), and a second stirring component (53) is provided inside the anaerobic fermentation chamber (5). A pretreatment chamber (6) is provided with a third stirring assembly (61), an adjustment port is provided on the upper part of the pretreatment chamber (6), and a first acid-base monitoring probe (64) is provided in the pretreatment chamber (6). The biogas delivery unit (7) has its input end connected to the anaerobic fermentation chamber (5) and its output end connected to the first culture chamber (1). The first liquid delivery unit (8) has its input end connected to the supernatant in the anaerobic fermentation chamber (5) and its output end connected to the pretreatment chamber (6). The second liquid delivery unit (9) has its input end located at the bottom of the pretreatment chamber (6) and its output end connected to the first culture chamber (1).

7. The microalgae and methanogenic bacteria separation and cultivation device according to claim 6, characterized in that, The biogas delivery unit (7) includes: Gas storage device (71); An air pump (72) is connected to the anaerobic fermentation chamber (5) at its input end and to the gas storage device (71) at its output end. The second aeration component (73) has its input end connected to the gas storage device (71), and the second aeration plate (731) of the second aeration component (73) is located at the bottom of the first culture chamber (1).

8. The microalgae and methanogenic bacteria separation and cultivation device according to claim 7, characterized in that, The biogas delivery unit (7) also includes: A gas flow rate detection device is installed in the pipeline of the second aeration component (73) to control the air flow rate of the second aeration component (73).

9. The microalgae and methanogenic bacteria separation and cultivation device according to claim 6, characterized in that, The first liquid delivery unit (8) includes: An infusion pipeline (81) is provided, with its two ends connected to the anaerobic fermentation chamber (5) and the pretreatment chamber (6), respectively. A pump body (82) is installed on the infusion pipeline (81) for conveying liquid in the infusion pipeline (81); A valve (83) is provided in the infusion line (81) to control the opening degree of the infusion line (81).

10. The microalgae and methanogenic bacteria separation and cultivation device according to claim 9, characterized in that, The first liquid delivery unit (8) further includes: A filter membrane (84) is disposed in the infusion line (81).

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