Nucleic acid qualitative detection device combining RAA amplification with electrochemical sensing

Abstract

The invention discloses a nucleic acid qualitative detection device combining RAA amplification with electrochemical sensing, and relates to the technical field of nucleic acid qualitative detection.The nucleic acid qualitative detection device comprises a box body, a box door is rotationally installed on the front portion of the box body, a guide rail is fixedly installed on the inner wall of the box body, a reagent plate is slidably installed in the guide rail, and detection equipment is arranged at the top of the inner wall of the box body; a stabilizing device, a protecting device and a locking device are arranged in the box body, the supporting blocks move to make contact with the reagent plate and support the reagent plate, the supporting force of the reagent plate is effectively increased, the reagent plate is prevented from deviating or deforming due to vibration or external force in the detection process, and therefore the accuracy of the position of the reagent plate is guaranteed; meanwhile, the damage or deformation of the reagent plate caused by external force is avoided, and the long-term stable use of the reagent plate is ensured.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid qualitative detection technology, specifically to a nucleic acid qualitative detection device that combines RAA amplification with electrochemical sensing. Background Technology

[0002] The RAA (Recombinase-Aided Amplification) amplification combined with electrochemical sensing is an innovative device for nucleic acid detection. It combines the advantages of RAA amplification technology and electrochemical sensors to achieve rapid, sensitive, and low-cost nucleic acid qualitative detection.

[0003] Patent publication number CN217127434U relates to the field of nucleic acid qualitative detection technology. This patent provides a nucleic acid qualitative detection device that provides intuitive display of results. The device includes a housing, within which are arranged a reaction module, a temperature control module, a detection module, an interpretation module, and a moving module. The reaction module includes at least one placement rack into which at least one detection tube is inserted. The detection module includes an optical sensor and a detection light source. The detection tube, driven by the moving module, moves between the optical sensor and the detection light source, ensuring that the detection light source, detection tube, and optical sensor are on the same optical path. The interpretation module includes an electrically connected data processor and an indicator light array. This nucleic acid qualitative detection device provides intuitive display of detection results via indicator lights, is easy to operate, and is suitable for real-time detection of various types and scenarios of nucleic acid samples.

[0004] In the aforementioned patent, the detection tube is moved between the optical sensor and the detection light source by the moving module, so that the detection light source, the detection tube, and the optical sensor are located on the same optical path; the interpretation module includes an electrically connected data processor and an indicator light array, but it is difficult to prevent the detection reagent plate from shaking during the detection process when performing qualitative detection of nucleic acid. This can easily cause the reagent plate to move due to vibration during the detection process, resulting in deviations in the detection results. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing, which solves the problems mentioned in the background section.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solution: a nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing, comprising: a box body, a door rotatably installed at the front of the box body, a guide rail fixedly installed on the inner wall of the box body, a reagent plate slidably installed inside the guide rail, a detection device provided at the top of the inner wall of the box body, and a stabilizing device, a protective device, and a locking device provided inside the box body;

[0007] The stabilizing device includes a sliding rod, a contact rod, a slide rail, a base plate, a first elastic telescopic rod, a support block, a first inclined block, a second elastic telescopic rod, and a stop block. The sliding rod is slidably installed on the bottom of the inner wall of the box. The contact rod is fixedly installed on the top of the sliding rod. The slide rail is fixedly installed on the inner wall of the box. The base plate is slidably installed inside the slide rail. The first elastic telescopic rod is fixedly installed on the top of the base plate. The support block is fixedly installed on the movable end of the first elastic telescopic rod. The first inclined block is fixedly installed on the bottom of the base plate. The second elastic telescopic rod is fixedly installed on the inner wall of the box. The stop block is fixedly installed on the movable end of the second elastic telescopic rod. When the box door is closed, the box door contacts and pushes the contact rod to start moving. The movement of the contact rod causes the sliding rod to start moving. The movement of the sliding rod contacts and pushes the first inclined block to start moving upward. The movement of the first inclined block causes the base plate to start moving along the extension direction of the slide rail. The movement of the base plate causes the first elastic telescopic rod to start moving upward. The movement of the first elastic telescopic rod causes the support block to start moving upward. The movement of the support block contacts the reagent plate and supports the reagent plate.

[0008] According to the above technical solution, the sides of the sliding rod and the inclined block are both set as inclined surfaces. The contact rod contacts the box door, and the stop block contacts the reagent plate. By setting the inclined surfaces, it is ensured that the sliding rod can smoothly push the inclined block when moving. By contact, it is ensured that the box door can push the contact rod. By contact, it is ensured that the stop block can support the reagent plate.

[0009] According to the above technical solution, the protective device includes a sliding column, a protective plate, and a first spring. The sliding column slides through the box body, and the protective plate is fixedly installed on the side of the sliding column away from the box body. A first spring is provided between the protective plate and the box body. When the sliding rod moves to contact and pushes the inclined block to move upward, it disengages from the sliding column and releases its restriction. Then the protective device starts to operate. When the detection device is working, the protective plate starts to move when the device is squeezed or collided. The movement of the protective plate drives the sliding column to move and squeeze the first spring.

[0010] According to the above technical solution, the protective device also includes a long rod, a sealing strip, a second inclined block, and a second spring. The long rod is fixedly installed at one end of the sliding column near the inside of the box. The sealing strip is slidably installed on the inner wall of the box. The second inclined block is fixedly installed on the back of the sealing strip. A second spring is provided between the sealing strip and the guide rail. When the sliding column starts to move, it drives the long rod to start to move. The long rod moves and contacts and pushes the second inclined block to start to move. The movement of the second inclined block drives the sealing strip to start to move. The sealing strip moves and makes complete contact with the box door and seals it.

[0011] According to the above technical solution, the side of the long rod that contacts the inclined block two is set as an inclined surface, and the sealing strip contacts the box door. By setting the inclined surface, it is ensured that the long rod can smoothly push the inclined block two when moving, and by contact, it is ensured that the sealing strip can close the box door.

[0012] According to the above technical solution, the locking device includes a fixed plate, two elastic telescopic rods (three in total), a connecting rod, a three-sided inclined block, an inclined rod, a four-sided elastic telescopic rod, a locking block, a hollow block, and a pulling block. The fixed plate is fixedly installed on the right side of the housing. The two elastic telescopic rods (three in total) are fixedly installed on the upper and lower sides of the fixed plate. The connecting rod is fixedly installed on the movable end of the elastic telescopic rod (three in total). The three-sided inclined block (three in total) is fixedly installed on the side of the connecting rod away from the movable end of the elastic telescopic rod (three in total). The inclined rod is fixedly installed on the side of the protective plate close to the housing. The four-sided elastic telescopic rod (four in total) is fixedly installed on the side of the connecting rod close to the three-sided elastic telescopic rod. The locking block... The hollow block is fixedly installed on the right side of the box door, and the pull block is fixedly installed on the right side of the locking block. The movement of the guard plate causes the sliding column to move and compress the first spring, which in turn causes the inclined rod to move. The inclined rod moves and contacts and pushes the inclined block to move. The movement of the inclined block causes the connecting rod to move. The movement of the connecting rod causes the elastic telescopic rod four to move and compress the movable end of the elastic telescopic rod three. The movement of the elastic telescopic rod four causes the locking block to move. The locking block moves and contacts the hollow block and enters its interior. After the locking block enters the hollow block, it restricts the box door.

[0013] According to the above technical solution, the sides of the inclined rod and the inclined block three that are in contact with each other are both set as inclined surfaces. The locking block is in contact with the hollow block. By setting the inclined surfaces, it is ensured that the inclined rod can smoothly push the inclined block three when it moves. By contact, it is ensured that the locking block can restrict the hollow block.

[0014] According to the above technical solution, the hollow block is hollow inside, and there are two card blocks. By setting it to be hollow, the card blocks can be moved into the hollow block. By setting two card blocks, better restriction can be achieved.

[0015] This invention provides a nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing. It has the following beneficial effects:

[0016] (1) This invention, by moving the support block to contact the reagent plate and support the reagent plate, effectively increases the support force of the reagent plate, prevents the reagent plate from shifting or deforming due to vibration or external force during the detection process, thereby ensuring the accuracy of the reagent plate position and avoiding affecting the accuracy of the detection results. At the same time, it avoids the reagent plate from being damaged or deformed due to external force, ensuring that it can be used stably for a long time. Under the restriction of the stop block and the movable end of the elastic telescopic rod, the movement position of the reagent plate is restricted to ensure that the reagent plate can be moved to the correct position, preventing the reagent plate from moving too much and causing the detection to fail.

[0017] (2) The invention uses the movement of the guard plate to drive the sliding column to start moving and squeeze the No. 1 spring, so as to reduce the impact force generated during squeezing or collision, effectively prevent external force from acting directly on the nucleic acid qualitative detection device, reduce equipment damage caused by impact or squeezing, improve the overall impact resistance of the device, extend the service life of the device, reduce failures caused by external physical interference, and at the same time effectively protect the device from external impact, ensuring the stability and accuracy of the detection process.

[0018] (3) The invention effectively prevents external air, dust, impurities or harmful gases from entering the device by moving the sealing strip to make full contact with the door and sealing it. It ensures that the device can remain sealed when squeezed or bumped, avoiding leakage of reagents or samples, causing environmental pollution or exposure of operators to hazardous substances. At the same time, it can effectively prevent changes in external environmental conditions, ensure that the device maintains a stable working environment, and improve the accuracy and repeatability of experiments.

[0019] (4) This invention restricts the door of the box after the card block enters the hollow block, effectively preventing the door from being accidentally opened or loosened due to external force, ensuring the sealing performance of the door, avoiding the sealing strip from being loosened or the sealing performance from decreasing, maintaining the stability of the internal environment, preventing the leakage of pollutants or reagents, and ensuring the safety of samples and reagents. The card block moves and disengages from the hollow block and releases the restriction on the door. Then, the staff pulls the door to open it, ensuring that the door can be opened manually when needed. This avoids the situation where the door cannot be opened when it is in a state of compression or collision, which would prevent the reagents or samples from being better preserved. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 This is a schematic diagram of the guide rail and reagent plate structure of the present invention;

[0022] Figure 3 This is a cross-sectional schematic diagram of the stabilization device structure of the present invention;

[0023] Figure 4 This is a schematic diagram of the elastic telescopic rod and support block structure of the present invention;

[0024] Figure 5 This is a cross-sectional schematic diagram of the protective device structure of the present invention;

[0025] Figure 6 For the present invention Figure 5 Enlarged schematic diagram of section A in the middle;

[0026] Figure 7This is a cross-sectional schematic diagram of the locking device structure of the present invention;

[0027] Figure 8 This is a schematic diagram of the three structures of the present invention: the fixed plate and the elastic telescopic rod.

[0028] In the diagram: 1. Box body; 2. Box door; 3. Guide rail; 4. Reagent plate; 5. Testing equipment; 6. Slide rod; 7. Contact rod; 8. Slide rail; 9. Base plate; 10. Elastic telescopic rod one; 11. Support block; 12. Inclined block one; 13. Elastic telescopic rod two; 14. Stop block; 151. Sliding column; 152. Guard plate; 153. Spring No. 1; 154. Long rod; 155. Sealing strip; 156. Inclined block two; 157. Spring No. 2; 161. Fixing plate; 162. Elastic telescopic rod three; 163. Connecting rod; 164. Inclined block three; 165. Inclined rod; 166. Elastic telescopic rod four; 167. Locking block; 168. Hollow block; 169. Pulling block. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] Please see Figures 1-8 One embodiment of the present invention is: a nucleic acid qualitative detection device for RAA amplification combined with electrochemical sensing, comprising: a box body 1, a box door 2 rotatably installed at the front of the box body 1, a guide rail 3 fixedly installed on the inner wall of the box body 1, a reagent plate 4 slidably installed inside the guide rail 3, a detection device 5 provided at the top of the inner wall of the box body 1, and a stabilizing device provided inside the box body 1.

[0031] The stabilizing device includes a slide rod 6, a contact rod 7, a slide rail 8, a base plate 9, a first elastic telescopic rod 10, a support block 11, a first inclined block 12, a second elastic telescopic rod 13, and a stop block 14. The slide rod 6 is slidably installed on the bottom of the inner wall of the housing 1, the contact rod 7 is fixedly installed on the top of the slide rod 6, the slide rail 8 is fixedly installed on the inner wall of the housing 1, the base plate 9 is slidably installed inside the slide rail 8, the first elastic telescopic rod 10 is fixedly installed on the top of the base plate 9, the support block 11 is fixedly installed on the movable end of the first elastic telescopic rod 10, and the first inclined block 12 is fixedly installed on the base plate. At the bottom, the second elastic telescopic rod 13 is fixedly installed on the inner wall of the box 1, and the stop block 14 is fixedly installed on the movable end of the second elastic telescopic rod 13. The stop block 14 moves to contact the reagent plate 4 through the support block 11 and supports the reagent plate 4, effectively increasing the support force of the reagent plate 4 and preventing the reagent plate 4 from shifting or deforming due to vibration or external force during the detection process. This ensures the accuracy of the position of the reagent plate 4, avoids affecting the accuracy of the detection results, and at the same time avoids the reagent plate 4 from being damaged or deformed due to external force, ensuring that it can be used stably for a long time.

[0032] The sides of the slide bar 6 and the inclined block 12 that are in contact with each other are both set as inclined surfaces. The contact rod 7 is in contact with the door 2, and the stop block 14 is in contact with the reagent plate 4. By setting the inclined surfaces, it is ensured that the slide bar 6 can smoothly push the inclined block 12 when it moves. By contact, it is ensured that the door 2 can push the contact rod 7. By contact, it is ensured that the stop block 14 can support the reagent plate 4.

[0033] In this embodiment, the following steps are required before starting the device for testing: First, a professional staff member must inspect the entire device. After the inspection, the nucleic acid qualitative test begins. The staff member places the nucleic acid sample to be tested on reagent plate 4, and then places reagent plate 4, containing the nucleic acid sample, inside the housing 1 via guide rail 3. After placement, the housing door 2 is closed, and the testing device 5 is started. The testing device 5 performs qualitative detection of the target nucleic acid using an electrochemical sensor. After the test is completed, the test data is transmitted to a designated device via a data transmission device. The staff member can then view the test data on the designated device. Simultaneously with the closing of housing door 2, housing door 2 contacts and pushes contact rod 7 to move. The movement of contact rod 7 causes slide rod 6 to move, and slide rod 6 contacts and pushes inclined block 12 to move upwards. The movement of inclined block 12 causes base plate 9 to move along... The slide rail 8 moves in its extension direction, and the base plate 9 moves, causing the elastic telescopic rod 10 to move upward. The elastic telescopic rod 10 moves, causing the support block 11 to move upward. The support block 11 moves and contacts the reagent plate 4, supporting the reagent plate 4 and effectively increasing the support force of the reagent plate 4. This prevents the reagent plate 4 from shifting or deforming due to vibration or external force during the detection process, thereby ensuring the accuracy of the position of the reagent plate 4 and avoiding affecting the accuracy of the detection results. At the same time, it avoids damage or deformation of the reagent plate 4 caused by external force, ensuring that it can be used stably for a long time. When the reagent plate 4 moves along the guide rail 3, it contacts and pushes the stop block 14 to start moving. The stop block 14 moves and squeezes the movable end of the elastic telescopic rod 13. Under the restriction of the stop block 14 and the movable end of the elastic telescopic rod 13, the movement position of the reagent plate 4 is restricted, ensuring that the reagent plate 4 can move to the correct position and preventing the reagent plate 4 from moving too much and causing the detection to fail.

[0034] Please see Figures 1-8 Based on the above embodiments, in another embodiment of the present invention, the housing 1 is provided with a protective device and a locking device.

[0035] The protective device includes a sliding column 151, a protective plate 152, and a first spring 153. The sliding column 151 slides through the housing 1, and the protective plate 152 is fixedly installed on the side of the sliding column 151 away from the housing 1. A first spring 153 is provided between the protective plate 152 and the housing 1. The movement of the protective plate 152 drives the sliding column 151 to move and compress the first spring 153, thereby reducing the impact force generated during compression or collision. This effectively prevents external forces from directly acting on the nucleic acid qualitative detection device, reduces equipment damage caused by impact or compression, improves the overall impact resistance of the device, extends the service life of the device, reduces malfunctions caused by external physical interference, and effectively protects the device from external impacts, ensuring the stability and accuracy of the detection process.

[0036] The protective device also includes a long rod 154, a sealing strip 155, a second inclined block 156, and a second spring 157. The long rod 154 is fixedly installed at one end of the sliding column 151 near the inside of the box 1. The sealing strip 155 is slidably installed on the inner wall of the box 1. The second inclined block 156 is fixedly installed on the back of the sealing strip 155. A second spring 157 is provided between the sealing strip 155 and the guide rail 3. The sealing strip 155 moves to make full contact with the box door 2 and seal it, effectively preventing external air, dust, impurities, or harmful gases from entering the device. This ensures that the device can remain sealed even when squeezed or impacted, avoiding reagent or sample leakage, environmental pollution, or operator exposure to hazardous substances. At the same time, it can effectively prevent changes in external environmental conditions, ensuring a stable working environment inside the device and improving the accuracy and repeatability of experiments.

[0037] The sides of the long rod 154 that come into contact with the inclined block 156 are both set as inclined surfaces. The sealing strip 155 contacts the box door 2. By setting the inclined surfaces, it is ensured that the long rod 154 can smoothly push the inclined block 156 when it moves. By contacting, it is ensured that the sealing strip 155 can close the box door 2.

[0038] The locking device includes a fixed plate 161, two elastic telescopic rods 162, a connecting rod 163, a ramp block 164, a ramp rod 165, an elastic telescopic rod 166, a locking block 167, a hollow block 168, and a pull block 169. The fixed plate 161 is fixedly installed on the right side of the housing 1. The two elastic telescopic rods 162 are fixedly installed on the upper and lower sides of the fixed plate 161. The connecting rod 163 is fixedly installed on the movable end of the elastic telescopic rod 162. The ramp block 164 is fixedly installed on the side of the connecting rod 163 away from the movable end of the elastic telescopic rod 162. The ramp rod 165 is fixedly installed on the side of the guard plate 152 close to the housing 1. The elastic telescopic rod 166 is fixedly installed on the side of the connecting rod 163 near the elastic telescopic rod 162. The locking block 167 is fixedly installed on the movable end of the elastic telescopic rod 166. The hollow block 168 is fixedly installed on the right side of the door 2. The pull block 169 is fixedly installed on the right side of the locking block 167. After the locking block 167 enters the hollow block 168, it restricts the door 2, effectively preventing the door 2 from being accidentally opened or loosened due to external force, ensuring the sealing performance of the door 2, preventing the sealing strip 155 from being loosened or the sealing performance from decreasing, maintaining the stability of the internal environment, preventing the leakage of pollutants or reagents, and ensuring the safety of samples and reagents.

[0039] The inclined rod 165 and the inclined block 164 are both set as inclined surfaces on their contact surfaces. The locking block 167 is in contact with the hollow block 168. By setting the inclined surfaces, it is ensured that the inclined rod 165 can smoothly push the inclined block 164 when it moves. By contact, it is ensured that the locking block 167 can restrict the hollow block 168.

[0040] The hollow block 168 is set to be hollow inside, and there are two card blocks 167. By setting it to be hollow, the card blocks 167 can be moved into the hollow block 168. By setting the number of card blocks 167 to two, better restriction can be achieved.

[0041] In this embodiment, as the sliding rod 6 moves to contact and pushes the inclined block 12 upward, it simultaneously disengages from the sliding column 151 and releases its restriction. The protective device then activates. When the detection device is subjected to pressure or impact during operation, the protective plate 152 moves, causing the sliding column 151 to move and compress the first spring 153. This reduces the impact force generated during pressure or impact, effectively preventing external forces from directly acting on the nucleic acid qualitative detection device, reducing equipment damage caused by impact or pressure, improving the overall impact resistance of the device, extending its service life, reducing malfunctions caused by external physical interference, and effectively protecting the device from external impacts. To ensure the stability and accuracy of the detection process, the sliding column 151 moves simultaneously with the long rod 154. The long rod 154 moves and contacts and pushes the inclined block 156 to move. The movement of the inclined block 156 causes the sealing strip 155 to move, making full contact with the door 2 and sealing it. This effectively prevents external air, dust, impurities, or harmful gases from entering the device, ensuring that the device remains sealed even when squeezed or impacted. This avoids reagent or sample leakage, preventing environmental pollution or operator exposure to hazardous substances. At the same time, it effectively prevents changes in external environmental conditions, ensuring a stable working environment inside the device and improving the accuracy and repeatability of the experiment.

[0042] As the guard plate 152 moves, it causes the sliding column 151 to move and compress the first spring 153, simultaneously causing the inclined rod 165 to move. The inclined rod 165 then contacts and pushes the inclined block 164 to move. The movement of the inclined block 164 causes the connecting rod 163 to move, which in turn causes the elastic telescopic rod 166 to move and compress the movable end of the elastic telescopic rod 162. The movement of the elastic telescopic rod 166 causes the locking block 167 to move. The locking block 167 then contacts the hollow block 168 and enters its interior. After entering the hollow block 168, the locking block 167 restricts the door 2, effectively preventing the door 2 from accidentally opening or loosening due to external force, thus ensuring the safety of the door 2. The sealing performance is ensured to prevent the sealing strip 155 from being loosened or its sealing performance from deteriorating, maintaining the stability of the internal environment, preventing the leakage of contaminants or reagents, and ensuring the safety of samples and reagents. When the device is squeezed or subjected to a collision, if the staff needs to open the door 2, the staff can manually pull the pull block 169 to start moving. The movement of the pull block 169 causes the locking block 167 to start moving. The locking block 167 moves and disengages from the hollow block 168, releasing the restriction on the door 2. Then the staff can pull the door 2 to open it, ensuring that the door 2 can be opened manually when needed. This avoids the situation where the door 2 cannot be opened when it is squeezed or subjected to a collision, which would prevent the reagents or samples from being properly preserved.

[0043] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid qualitative detection device combining RAA amplification and electrochemical sensing, characterized in that, include: Box (1), the front of the box (1) is rotatably installed with a box door (2), the inner wall of the box (1) is fixedly installed with a guide rail (3), the inside of the guide rail (3) is slidably installed with a reagent plate (4), the top of the inner wall of the box (1) is provided with a detection device (5), and the inside of the box (1) is provided with a stabilizing device, a protective device and a locking device; The stabilizing device includes a slide rod (6), a contact rod (7), a slide rail (8), a base plate (9), an elastic telescopic rod one (10), a support block (11), an inclined block one (12), an elastic telescopic rod two (13), and a stop block (14). The slide rod (6) is slidably installed on the bottom of the inner wall of the box (1). The contact rod (7) is fixedly installed on the top of the slide rod (6). The slide rail (8) is fixedly installed on the inner wall of the box (1). The base plate (9) is slidably installed inside the slide rail (8). The elastic telescopic rod one (10) is fixedly installed on the top of the base plate (9). The support block (11) is fixedly installed on the movable end of the elastic telescopic rod one (10). The inclined block one (12) is fixedly installed on the bottom of the base plate (9). The elastic telescopic rod two (13) is fixedly installed on the inner wall of the box (1). The stop block (14) is fixedly installed on the movable end of the elastic telescopic rod two (13).

2. The nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing according to claim 1, characterized in that: The sides of the slide bar (6) that are in contact with the inclined block (12) are both set as inclined surfaces. The contact rod (7) is in contact with the box door (2). The stop block (14) is in contact with the reagent plate (4).

3. The nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing according to claim 1, characterized in that: The protective device includes a sliding column (151), a protective plate (152), and a first spring (153). The sliding column (151) slides through the box (1). The protective plate (152) is fixedly installed on the side of the sliding column (151) away from the box (1). The first spring (153) is provided between the protective plate (152) and the box (1).

4. The nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing according to claim 3, characterized in that: The protective device also includes a long rod (154), a sealing strip (155), a second inclined block (156), and a second spring (157). The long rod (154) is fixedly installed on one end of the sliding column (151) near the inside of the box (1). The sealing strip (155) is slidably installed on the inner wall of the box (1). The second inclined block (156) is fixedly installed on the back of the sealing strip (155). A second spring (157) is provided between the sealing strip (155) and the guide rail (3).

5. The nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing according to claim 4, characterized in that: The side of the long rod (154) that contacts the inclined block (156) is set as an inclined surface, and the sealing strip (155) contacts the box door (2).

6. The nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing according to claim 1, characterized in that: The locking device includes a fixed plate (161), two elastic telescopic rods (162), a connecting rod (163), a three-sided inclined block (164), an inclined rod (165), an elastic telescopic rod (166), a locking block (167), a hollow block (168), and a pulling block (169). The fixed plate (161) is fixedly installed on the right side of the box (1), the two elastic telescopic rods (162) are fixedly installed on the upper and lower sides of the fixed plate (161), the connecting rod (163) is fixedly installed on the movable end of the elastic telescopic rod (162), and the three-sided inclined block (164) is fixedly installed on the lower and upper sides of the fixed plate (165). 164) The connecting rod (163) is fixedly installed on the side away from the movable end of the elastic telescopic rod three (162). The inclined rod (165) is fixedly installed on the side of the guard plate (152) close to the box (1). The elastic telescopic rod four (166) is fixedly installed on the side of the connecting rod (163) close to the elastic telescopic rod three (162). The locking block (167) is fixedly installed on the movable end of the elastic telescopic rod four (166). The hollow block (168) is fixedly installed on the right side of the box door (2). The pull block (169) is fixedly installed on the right side of the locking block (167).

7. The nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing according to claim 6, characterized in that: The inclined rod (165) and the inclined block three (164) are both set as inclined surfaces on the side that are in contact with each other, and the card block (167) is in contact with the hollow block (168).

8. The nucleic acid qualitative detection device based on RAA amplification combined with electrochemical sensing according to claim 7, characterized in that: The hollow block (168) is hollow inside, and the card block (167) consists of two blocks.

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