Detection structure, detection method and bionic simulation organ cultivation tank

Abstract

The invention discloses a detection structure, a detection method and a bionic simulation organ cultivation tank, and relates to the field of detection equipment. The circulating detection pipe is connected and mounted on the outer wall of the bionic simulation organ cultivation tank, cultivation liquid in the bionic simulation organ cultivation tank is driven by the hydraulic pumping unit to circularly flow in the circulating detection pipe, and the pH value of the cultivation liquid flowing through the circulating detection pipe is continuously detected by the pH value detection sensor. Compared with sampling detection, the detection process is more convenient and faster, and due to the fact that the cultivation liquid in the bionic simulation organ cultivation tank continuously and circularly flows for detection, the cultivation liquid in the cultivation tank can be comprehensively detected, and the pH value detection precision of the cultivation liquid is improved. The circulating detection pipe is connected with the cleaning assembly, so that when the pH value detection is completed, cleaning liquid can be conveyed into the circulating detection pipe through the cleaning assembly, and the circulating detection pipe and a detection probe of the pH value detection sensor are cleaned.

Description

Technical Field

[0001] This invention belongs to the field of testing equipment, and more specifically, relates to a testing structure, a testing method, and a biomimetic simulated organ culture vessel. Background Technology

[0002] Bezoar is a gallstone found in the gallbladder of bovine animals (Bovidae), belonging to the class Mammalia, phylum Chordata, and has high medicinal value. However, the production of natural bezoar is limited, and most of it currently used is artificial bezoar. Based on the formation mechanism of natural bezoar, modern biomimetic organoid technology and traditional pill-making processes are used to prepare in vitro cultured bezoar with the same composition and properties as natural bezoar. This process includes four steps: strain selection and culture, calcification, cultivation, and shaping. Selected and cultured E. coli and bovine bile are injected into a fermentation tank for fermentation. Bilirubin and saturated calcium hydroxide solution are then added to prepare a complex bilirubin calcium salt. Separately, fermented bovine bile, complex bilirubin calcium, bovine deoxycholic acid, cholic acid, and trace elements such as magnesium sulfate and zinc sulfate are added to a biomimetic organoid culture tank. Using a fully automated biomimetic organoid function device, the cultivation temperature, pH, and stirring speed are controlled. After 10-12 hours of cultivation, bezoar granules and solid bezoar are obtained.

[0003] When cultivating bezoar using biomimetic organoid culture tanks, it is necessary to detect the pH value inside the tank. Currently, two common methods are: 1) Directly installing a pH sensor on the culture tank for real-time monitoring. However, this method involves the sensor probe being constantly immersed in acidic culture medium, leading to corrosion and reducing the sensor's lifespan and accuracy. 2) Installing a plunger pump or similar sampling structure on the culture tank for periodic sampling. However, this method is cumbersome, inefficient, and requires a limited sample size, failing to comprehensively monitor the culture medium and affecting accuracy. Furthermore, the samples are discarded after testing, wasting culture medium and increasing bezoar production costs. Summary of the Invention

[0004] In response to the problems in related technologies, this invention proposes a detection structure, a detection method, and a biomimetic simulated organ culture vessel to overcome the aforementioned technical problems existing in the prior art.

[0005] To solve the above-mentioned technical problems, the present invention is achieved through the following technical solution:

[0006] This invention discloses a biomimetic organ culture tank, comprising a detection structure, a control module, a temperature regulation unit, a pH regulation unit, a stirring unit, and an oxygen supply unit. The control module intelligently regulates the detection structure and each unit to simulate the ecological environment of a bovine gallbladder. The stirring and oxygen supply units maintain the culture medium in an agitated state through stirring and oxygen input. The pH regulation unit can deliver acidic or alkaline regulating solutions to the biomimetic organ culture tank based on the pH of the culture medium detected by the detection structure, thereby adjusting the pH value to be between 6.5 and 7.0. The temperature regulation unit can detect and adjust the reaction temperature within the biomimetic organ culture tank online.

[0007] Furthermore, the biomimetic organ culture tank also includes an automatic quantitative feeding system and an online cleaning and disinfection system, which can automatically add drugs in quantitative quantities during the reaction and automatically clean and disinfect the biomimetic organ culture tank after the reaction is completed.

[0008] Furthermore, the detection structure includes a pH detection component, which includes a circulation detection tube. Both ends of the circulation detection tube are connected to a biomimetic organ culture tank. A pH detection sensor and a hydraulic pumping unit are connected and installed on the circulation detection tube. The hydraulic pumping unit can drive the culture medium in the biomimetic organ culture tank to circulate within the circulation detection tube. The detection probe of the pH detection sensor extends into the interior of the circulation detection tube and can detect the pH of the culture medium flowing through the circulation detection tube.

[0009] The detection structure also includes a cleaning component and a flow channel adjustment component. The cleaning component can deliver cleaning solution into the circulating detection tube after the pH of the culture medium is detected, so as to clean the circulating detection tube and the detection probe of the pH detection sensor.

[0010] The flow channel adjustment assembly includes a feed sealing valve group and a cleaning sealing valve group. The cleaning sealing valve group can seal and isolate the circulation detection tube from the cleaning assembly when the culture medium is being detected. The feed sealing valve group can delay the isolation between the circulation detection tube and the biomimetic organ culture tank when the cleaning liquid enters the circulation detection tube, so that the culture medium in the circulation detection tube can flow back into the biomimetic organ culture tank.

[0011] Furthermore, the pH detection component also includes a sampling conduit and a reflux conduit, both of which are connected and installed on the biomimetic simulated organ culture tank, and the circulation detection tube is connected and installed between the sampling conduit and the reflux conduit.

[0012] Furthermore, the cleaning assembly includes an inlet conduit and a drain pipe, which are respectively connected to the two ends of the circulation detection pipe, and one end of the inlet conduit is connected to the cleaning fluid tank.

[0013] Furthermore, the hydraulic pumping unit includes a hydraulic pump, which is connected and installed on the circulation detection pipe, and the hydraulic pump is a bidirectional hydraulic pump.

[0014] Furthermore, the feed sealing valve assembly includes a reflux pipe sealing valve and a sampling pipe sealing valve, which are respectively connected and installed on the reflux conduit and the sampling conduit, and the sampling pipe sealing valve is a time-delay sealing valve. The cleaning sealing valve assembly includes an inlet pipe sealing valve and a drain pipe sealing valve, which are respectively connected and installed on the inlet conduit and the drain pipe, and the drain pipe sealing valve is a pressure valve.

[0015] Furthermore, the sampling tube sealing valve has a sampling valve chamber and a pressure chamber inside, and an elastic diaphragm is fixedly installed between the sampling valve chamber and the pressure chamber. A valve chamber partition for isolating the valve inlet and outlet is installed inside the sampling valve chamber. A sampling valve hole is opened on the valve chamber partition. A sampling valve core is sealed and snapped into the sampling valve hole. A sampling valve rod is fixedly installed at the top of the sampling valve core. The top of the sampling valve rod is fixedly connected to the middle of the elastic diaphragm.

[0016] A flow guide tube is connected to one end of the sampling tube sealing valve that is connected to the circulation detection tube. One end of the flow guide tube is connected to the pressure chamber. A flow regulating valve is also connected to the flow guide tube.

[0017] Furthermore, the upper end of the sampling valve rod extends upward into the interior of the pressure chamber, and a limit block is fixedly installed at the top end of the sampling valve rod, with a limit hole provided on the limit block;

[0018] The pressure chamber is fixedly installed with a limiting cylinder that slides into the upper end of the sampling valve rod. The limiting cylinder is provided with a telescopic groove. A slider is slidably fitted inside the telescopic groove. A return spring is installed on the inner end of the slider. A limiting post that can slide into the limiting hole is fixedly installed on the outer end of the slider.

[0019] Furthermore, the drain pipe sealing valve has a drain valve chamber inside, and a drain baffle plate for separating the two ends of the valve is fixedly installed inside the drain valve chamber. A drain valve hole is opened on the drain baffle plate, and a drain valve core is sealed and fitted inside the drain valve hole. A drain valve rod is fixedly installed at the top of the drain valve core. A limiting diaphragm fixedly connected to the drain valve rod is installed at the upper end of the drain valve chamber. A spring seat that slides into the top of the drain valve rod is fixedly installed at the top of the drain valve chamber. A pressure spring that abuts against the top of the drain valve rod is installed inside the spring seat.

[0020] The present invention also discloses a detection method, the specific steps of which are as follows:

[0021] First, adjust the feed sealing valve group to the open state and the cleaning sealing valve group to the closed state. Then, drive the culture medium in the bionic simulated organ culture tank to circulate in the circulation detection tube through the hydraulic pumping unit. At this time, the acid-base detection sensor can continuously detect the acid-base of the culture medium flowing through the circulation detection tube.

[0022] Once the pH test is complete, the cleaning sealing valve assembly is opened, allowing the cleaning components to begin supplying cleaning fluid into the circulating detection tube to clean the circulating detection tube and the detection probe of the pH sensor. Meanwhile, the feed sealing valve assembly is closed after a delay, allowing any residual culture medium in the circulating detection tube to flow back into the biomimetic organ culture tank under the pressure of the cleaning fluid.

[0023] The present invention has the following beneficial effects:

[0024] 1. In this invention, a circulation detection tube is connected and installed on the outer wall of the bionic simulated organ culture tank. A pH sensor and a hydraulic pumping unit are installed on the circulation detection tube. The hydraulic pumping unit drives the culture medium inside the bionic simulated organ culture tank to circulate within the circulation detection tube. The pH sensor continuously detects the pH of the culture medium flowing through the circulation detection tube. Compared to sampling detection, this detection process is more convenient and faster. Furthermore, because the culture medium inside the bionic simulated organ culture tank is continuously circulated, a comprehensive detection of the culture medium can be performed, thereby improving the accuracy of pH detection.

[0025] 2. In this invention, a cleaning assembly is connected to the circulating detection tube. When the pH test is completed, the cleaning assembly can deliver cleaning fluid into the circulating detection tube to clean the circulating detection tube and the detection probe of the pH sensor. This not only keeps the circulating detection tube clean, but also removes the culture solution adhering to the detection probe of the pH sensor, preventing corrosion of the detection probe and improving the service life and detection accuracy of the pH sensor.

[0026] 3. In this invention, when the cleaning sealing valve assembly is opened, allowing the cleaning assembly to begin conveying cleaning fluid into the circulating detection tube for cleaning, the feed sealing valve assembly installed between the circulating detection tube and the bionic simulated organ culture tank closes after a delay. This allows the culture fluid remaining in the circulating detection tube to flow back into the bionic simulated organ culture tank under the pressure of the cleaning fluid, thereby reducing the loss of culture fluid during the detection process and lowering the production cost of bezoar.

[0027] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0028] To more clearly illustrate the technical solutions of the embodiments of the invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the invention. For those skilled in the art, the drawings can be obtained from these drawings without creative effort.

[0029] Figure 1 This is one of the three-dimensional structural schematic diagrams of the detection structure of the present invention;

[0030] Figure 2 For the present invention Figure 1 A magnified schematic diagram of the structure at point A;

[0031] Figure 3 This is a second three-dimensional structural schematic diagram of the detection structure of the present invention;

[0032] Figure 4 For the present invention Figure 3 A magnified schematic diagram of the structure at point B;

[0033] Figure 5 This is the third three-dimensional structural schematic diagram of the detection structure of the present invention;

[0034] Figure 6 For the present invention Figure 5 A magnified schematic diagram of the structure at point C;

[0035] Figure 7 For the present invention Figure 5 A magnified schematic diagram of the structure at point D;

[0036] Figure 8 This is a front view of the detection structure of the present invention;

[0037] Figure 9 For the present invention Figure 8 A magnified schematic diagram of the structure at point E.

[0038] In the diagram: 1. pH detection assembly; 11. Circulation detection tube; 12. pH sensor; 13. Hydraulic pump; 14. Sampling conduit; 15. Return conduit; 2. Cleaning assembly; 21. Inlet conduit; 22. Drain pipe; 3. Flow channel adjustment assembly; 31. Return pipe sealing valve; 32. Inlet pipe sealing valve; 33. Sampling tube sealing valve; 34. Drain pipe sealing valve; 35. Pressure chamber; 36. Guide pipe; 37. Flow regulating valve; 38. Sampling valve chamber; 39. Valve 310. Sampling valve orifice; 311. Sampling valve core; 312. Sampling valve stem; 313. Elastic diaphragm; 314. Limiting cylinder; 315. Drain valve cavity; 316. Drain baffle; 317. Drain valve orifice; 318. Drain valve core; 319. Drain valve stem; 320. Spring seat; 321. Pressure spring; 322. Limiting diaphragm; 323. Limiting block; 324. Limiting hole; 325. Telescopic slide; 326. Return spring; 327. Sliding block; 328. Limiting post. Detailed Implementation

[0039] The technical solutions of the embodiments of the invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the invention, and not all embodiments. Based on the embodiments of the invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the invention.

[0040] In the description of this invention, it should be understood that the terms "opening", "upper", "lower", "top", "middle", "inner", etc., which indicate orientation or positional relationship, are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the invention.

[0041] Example 1

[0042] Please see Figure 1 , Figure 2As shown, this invention is a detection structure that can be applied to a biomimetic organ culture tank. The detection structure includes a pH detection component 1, which is connected to the biomimetic organ culture tank. The pH detection component 1 includes a circulation detection tube 11, both ends of which are connected to the biomimetic organ culture tank. A pH sensor 12 and a hydraulic pumping unit are connected and installed on the circulation detection tube 11. The hydraulic pumping unit drives the culture medium inside the biomimetic organ culture tank to circulate within the circulation detection tube 11. The detection probe of the pH sensor 12 extends into the circulation detection tube 11, enabling it to detect the pH of the culture medium flowing through the circulation detection tube 11. The detection structure also includes a cleaning component 2 and a flow channel adjustment component 3. The cleaning component 2 can... After the pH of the culture medium is detected, cleaning fluid is supplied to the circulation detection tube 11 to clean the circulation detection tube 11 and the detection probe of the pH detection sensor 12. The flow channel regulating assembly 3 includes a feed sealing valve group and a cleaning sealing valve group. The cleaning sealing valve group can seal and isolate the circulation detection tube 11 from the cleaning assembly 2 when the culture medium is flowing. The feed sealing valve group can delay the isolation between the circulation detection tube 11 and the bionic simulated organ culture tank when the cleaning fluid enters the circulation detection tube 11, so that the culture medium in the circulation detection tube 11 can flow back into the bionic simulated organ culture tank.

[0043] When the pH of the culture medium in the biomimetic organ culture tank is tested, the feed sealing valve group is first adjusted to the open state, and the cleaning sealing valve group is adjusted to the closed state. Then, the culture medium in the biomimetic organ culture tank is driven to circulate in the circulation detection tube 11 by the hydraulic pumping unit. At this time, the pH detection sensor 12 can continuously detect the pH of the culture medium flowing through the circulation detection tube 11. When the pH test is completed, the cleaning sealing valve group is adjusted to the open state, so that the cleaning component 2 starts to deliver cleaning fluid into the circulation detection tube 11 to clean the circulation detection tube 11 and the detection probe of the pH detection sensor 12. The feed sealing valve group is closed after a delay so that the culture medium remaining in the circulation detection tube 11 can flow back into the biomimetic organ culture tank through the feed sealing valve group under the squeezing and conveying of the cleaning fluid.

[0044] The pH sensor 12 continuously monitors the pH of the culture medium flowing through the circulation detection tube 11. Compared to sampling, this method is more convenient and faster. Furthermore, the continuous circulation of the culture medium within the biomimetic organ culture tank allows for comprehensive monitoring, improving the accuracy of pH detection. The cleaning assembly 2 delivers cleaning fluid to the circulation detection tube 11, cleaning both the tube and the sensor's probe. This not only keeps the tube clean but also removes any adhering culture medium from the sensor's probe, preventing corrosion and damage, thus extending its lifespan and improving its accuracy. By delaying the closure of the feed sealing valve assembly, residual culture medium in the circulation detection tube 11 is returned to the biomimetic organ culture tank under the pressure of the cleaning fluid, reducing culture medium loss during monitoring and lowering production costs.

[0045] Example 2

[0046] Please see Figure 1 , Figure 2 As shown, the difference between this embodiment and the above embodiment is that the pH detection component 1 further includes a sampling conduit 14 and a return conduit 15, both of which are connected and installed on the biomimetic simulated organ culture tank, and the circulation detection tube 11 is connected and installed between the sampling conduit 14 and the return conduit 15; the cleaning component 2 includes an inlet conduit 21 and a drain conduit 22, which are respectively connected and installed at both ends of the circulation detection tube 11, and one end of the inlet conduit 21 is connected to the cleaning solution tank;

[0047] The hydraulic pumping unit includes a hydraulic pump 13, which is connected and installed on the circulation detection pipe 11, and the hydraulic pump 13 is a bidirectional hydraulic pump; the feed sealing valve group includes a return pipe sealing valve 31 and a sampling pipe sealing valve 33, which are respectively connected and installed on the return conduit 15 and the sampling conduit 14, and the sampling pipe sealing valve 33 is a delayed sealing valve; the cleaning sealing valve group includes an inlet pipe sealing valve 32 and a drain pipe sealing valve 34, which are respectively connected and installed on the inlet conduit 21 and the drain pipe 22, and the drain pipe sealing valve 34 is a pressure valve;

[0048] When performing pH testing, the reflux pipe sealing valve 31 and sampling pipe sealing valve 33 are opened, while the inlet pipe sealing valve 32 and drain pipe sealing valve 34 are closed. Then, the hydraulic pump 13 is activated. The hydraulic pump 13 hydraulically draws the culture medium from the biomimetic organ culture tank through the sampling conduit 14, causing the culture medium to circulate along the sampling conduit 14, the circulation detection pipe 11, and the reflux pipe 15. Meanwhile, the pH sensor 12 continuously detects the pH of the culture medium as it flows through the circulation detection pipe 11. When cleaning is required after pH testing, the reflux pipe sealing valve 31 is closed, and the inlet pipe sealing valve 32 is opened. Simultaneously, the hydraulic pump 13 reverses its flow, pumping the cleaning solution through the inlet conduit 21. The cleaning solution in the tank is gradually delivered to the circulation detection tube 11 to clean the detection probe of the circulation detection tube 11 and the pH detection sensor 12. At the same time, the culture solution remaining in the circulation detection tube 11 is gradually returned to the bionic simulated organ culture tank by the squeezing and delivery of the cleaning solution through the inlet pipe sealing valve 32 and the sampling conduit 14. Simultaneously, the inlet pipe sealing valve 32 automatically closes after a delay under the return hydraulic drive to prevent the cleaning solution in the circulation detection tube 11 from flowing into the bionic simulated organ culture tank. Afterwards, as the cleaning solution is continuously delivered, the hydraulic pressure in the circulation detection tube 11 increases, causing the drain pipe sealing valve 34 to open automatically under the hydraulic drive, thereby discharging the cleaned wastewater through the drain pipe 22 and the drain pipe sealing valve 34.

[0049] Example 3

[0050] Please see Figures 3-9 As shown, the difference between this embodiment and the above embodiment is that the sampling tube sealing valve 33 has a sampling valve chamber 38 and a pressure chamber 35 inside. An elastic diaphragm 313 is fixedly installed between the sampling valve chamber 38 and the pressure chamber 35. A valve chamber partition 39 for isolating the valve inlet and outlet is installed inside the sampling valve chamber 38. A sampling valve hole 310 is opened on the valve chamber partition 39. A sampling valve core 311 is sealed and snapped into the sampling valve hole 310. A sampling valve stem 312 is fixedly installed at the top of the sampling valve core 311. The top of the sampling valve stem 312 is fixedly connected to the middle of the elastic diaphragm 313. A guide pipe 36 is connected to one end of the sampling tube sealing valve 33 that is connected to the circulation detection pipe 11. One end of the guide pipe 36 is connected to the pressure chamber 35. A flow regulating valve 37 is also connected to the guide pipe 36.

[0051] When the culture medium is being tested, the sampling valve core 311 moves upward and opens under the negative pressure suction of the culture medium hydraulic pressure and the hydraulic pump 13, allowing the culture medium to flow through the sampling valve orifice 310 for normal delivery. At the same time, the sampling valve rod 312 drives the elastic diaphragm 313 to deform and bulge upward. When the test is completed and the cleaning is finished, the culture medium in the circulation test tube 11 flows back to the bionic simulated organ culture tank through the sampling tube sealing valve 33. At the same time, some of the culture medium is gradually delivered to the pressure chamber 35 through the guide tube 36, so that the hydraulic pressure in the pressure chamber 35 gradually increases. At this time, the elastic diaphragm 313 gradually deforms and resets downward under the action of hydraulic pressure, thereby driving the sampling valve rod 312 and the sampling valve core 311 to move downward until the sampling valve core 311 seals and closes the sampling valve orifice 310, and the culture medium no longer flows back.

[0052] The flow rate of the culture medium delivered to the pressure chamber 35 via the guide tube 36 can be adjusted by regulating the opening size of the flow regulating valve 37. This, in turn, regulates the speed at which the sampling valve core 311 descends to seal the sampling valve orifice 310, thereby adjusting the delayed closing speed of the sampling tube sealing valve 33. This ensures that as much culture medium as possible is returned to the biomimetic organ culture tank while preventing the cleaning solution from returning to the biomimetic organ culture tank and causing contamination of the culture medium.

[0053] Furthermore, the upper end of the sampling valve rod 312 extends upward into the interior of the pressure chamber 35, and a limiting block 323 is fixedly installed at the top end of the sampling valve rod 312, with a limiting hole 324 provided on the limiting block 323; a limiting cylinder 314 is fixedly installed at the top end of the pressure chamber 35, which slides into the upper end of the sampling valve rod 312; a telescopic groove 325 is provided inside the limiting cylinder 314; a slider 327 is slidably fitted inside the telescopic groove 325; a return spring 326 is installed at the inner end of the slider 327; and a limiting post 328 that can slide into the limiting hole 324 is fixedly installed at the outer end of the slider 327.

[0054] When the sampling valve rod 312 moves upward during the culture medium flow detection process, it drives the limiting block 323 to move upward synchronously, causing the limiting post 328 to insert into the limiting hole 324 on the limiting block 323. This pin-connects and limits the sampling valve rod 312. When the culture medium flows back during the cleaning process, the sampling valve rod 312 and the sampling valve core 311 will not immediately move downward because the sampling valve rod 312 is pin-connected and limited. This allows the sampling valve orifice 310 to maintain a large opening, thereby increasing the reflux speed and flow rate of the culture medium. The pressure of the culture medium in the pressure chamber 35 on the elastic diaphragm 313 is greater than the locking force between the limiting post 328 and the limiting hole 324. Under the guidance of the arc surface at the end of the limiting post 328, the limiting block 323 pushes the limiting post 328 into the telescopic groove 325 to receive and retract, releasing the pin connection limit on the limiting block 323. At this time, the elastic diaphragm 313 deforms downward under the action of the hydraulic pressure, and drives the sampling valve rod 312 and the sampling valve core 311 to move downward, sealing and closing the sampling valve hole 310 to prevent the culture medium from continuing to flow back.

[0055] By limiting and locking the limiting post 328 and the limiting block 323, the sampling valve core 311 can be prevented from descending too early to seal the sampling valve hole 310, so that the culture medium in the circulating detection tube 11 has enough reflux time, thereby reducing the loss of culture medium during the detection process.

[0056] Example 4

[0057] Please see Figure 5 , Figure 7 As shown, the difference between this embodiment and the above embodiment is that the drain pipe sealing valve 34 has a drain valve cavity 315 inside, a drain baffle 316 for separating the two ends of the valve is fixedly installed inside the drain valve cavity 315, a drain valve hole 317 is opened on the drain baffle 316, a drain valve core 318 is sealed and fitted inside the drain valve hole 317, a drain valve rod 319 is fixedly installed at the top of the drain valve core 318, a limiting diaphragm 322 fixedly connected to the drain valve rod 319 is installed at the upper end of the drain valve cavity 315, a spring seat 320 that slides into the top of the drain valve rod 319 is fixedly installed at the top of the drain valve cavity 315, and a pressure spring 321 that abuts against the top of the drain valve rod 319 is installed inside the spring seat 320;

[0058] During cleaning and sewage discharge, the sewage discharge valve core 318 initially seals the sewage discharge valve hole 317 under the elastic force of the pressure spring 321, thereby keeping the sewage discharge pipe sealing valve 34 in a sealed state and preventing the culture solution from being discharged outward from the sewage discharge pipe sealing valve 34 and the sewage discharge pipe 22 in the early stage of cleaning and sewage discharge. However, after the sampling pipe sealing valve 33 is closed, the hydraulic pressure on the sewage discharge valve core 318 increases with the continuous input of cleaning solution. At this time, the sewage discharge valve core 318 moves upward under the action of hydraulic thrust, causing the sewage discharge valve hole 317 to open. After that, the cleaning sewage is continuously discharged outward through the sewage discharge pipe sealing valve 34 and the sewage discharge pipe 22.

[0059] Example 5

[0060] This embodiment discloses a detection method, the specific steps of which are as follows:

[0061] First, adjust the feed sealing valve group to the open state and the cleaning sealing valve group to the closed state. Then, drive the culture medium in the bionic simulated organ culture tank to circulate in the circulation detection tube 11 through the hydraulic pumping unit. At this time, the acid-base detection sensor 12 can continuously detect the acid-base of the culture medium flowing through the circulation detection tube 11.

[0062] Once the pH test is complete, the cleaning sealing valve assembly is opened, allowing the cleaning component 2 to begin supplying cleaning fluid into the circulating detection tube 11 to clean the circulating detection tube 11 and the detection probe of the pH sensor 12. Meanwhile, the feed sealing valve assembly is closed after a delay, allowing any remaining culture medium in the circulating detection tube 11 to flow back into the biomimetic simulated organ culture tank under the pressure of the cleaning fluid.

[0063] Example 6

[0064] This embodiment discloses a biomimetic organ culture tank, including a detection structure, a control module, a temperature regulation unit, a pH regulation unit, a stirring unit, and an oxygen supply unit. The control module intelligently regulates the detection structure and each unit to simulate the ecological environment of a bovine gallbladder. The stirring and oxygen supply units keep the culture medium in an agitated state through stirring and oxygen input. The pH regulation unit can deliver acidic or alkaline regulating solution into the biomimetic organ culture tank according to the pH of the culture medium detected by the detection structure to adjust the pH value between 6.5 and 7.0. The temperature regulation unit can detect and adjust the reaction temperature in the biomimetic organ culture tank online.

[0065] In some embodiments, the biomimetic organ culture tank also includes an automatic quantitative feeding system and an online cleaning and disinfection system, which can automatically add drugs in quantitative quantities during the reaction and automatically clean and disinfect the biomimetic organ culture tank after the reaction is completed.

[0066] The control module uses a PLC controller from the prior art. The temperature regulation unit includes a heating jacket, a water supply pipe, and a temperature detection sensor located on the outer ring of the bionic organ culture tank. The temperature detection sensor detects the temperature inside the bionic organ culture tank, and hot water is supplied to the heating jacket through the water supply pipe to regulate the temperature of the bionic organ culture tank. The stirring unit includes stirring blades and a stirring motor for driving the stirring blades to rotate. The oxygen supply unit includes an oxygen supply pipe connected to the bionic organ culture tank to supply oxygen to the bionic organ culture tank.

[0067] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0068] The preferred embodiments of the invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention.

Claims

1. A detection structure, comprising an acid-base detection component, characterized in that: The pH detection component includes a circulation detection tube, both ends of which are connected to a biomimetic organ culture tank. A pH detection sensor and a hydraulic pumping unit are connected and installed on the circulation detection tube. The hydraulic pumping unit can drive the culture medium in the biomimetic organ culture tank to circulate within the circulation detection tube. The detection probe of the pH detection sensor extends into the interior of the circulation detection tube and can detect the pH of the culture medium flowing through the circulation detection tube. The detection structure also includes a cleaning component and a flow channel adjustment component. The cleaning component can deliver cleaning solution into the circulating detection tube after the pH of the culture medium is detected, so as to clean the circulating detection tube and the detection probe of the pH detection sensor. The flow channel adjustment assembly includes a feed sealing valve group and a cleaning sealing valve group. The cleaning sealing valve group can seal and isolate the circulation detection tube from the cleaning assembly when the culture medium is being detected. The feed sealing valve group can delay the isolation between the circulation detection tube and the biomimetic organ culture tank when the cleaning liquid enters the circulation detection tube, so that the culture medium in the circulation detection tube can flow back into the biomimetic organ culture tank.

2. The detection structure according to claim 1, characterized in that: The pH detection component also includes a sampling catheter and a reflux catheter, both of which are connected and installed on the biomimetic simulated organ culture tank, and the circulation detection tube is connected and installed between the sampling catheter and the reflux catheter.

3. The detection structure according to claim 2, characterized in that: The cleaning assembly includes an inlet conduit and a drain pipe, which are respectively connected to the two ends of the circulation detection pipe, and one end of the inlet conduit is connected to the cleaning fluid tank.

4. The detection structure according to claim 1, characterized in that: The hydraulic pumping unit includes a hydraulic pump, which is connected and installed on the circulation detection pipe, and the hydraulic pump is a bidirectional hydraulic pump.

5. The detection structure according to claim 3, characterized in that: The feed sealing valve assembly includes a reflux pipe sealing valve and a sampling pipe sealing valve, which are respectively connected and installed on the reflux conduit and the sampling conduit. The sampling pipe sealing valve is a time-delay sealing valve. The cleaning sealing valve assembly includes an inlet pipe sealing valve and a drain pipe sealing valve, which are respectively connected and installed on the inlet conduit and the drain pipe. The drain pipe sealing valve is a pressure valve.

6. The detection structure according to claim 5, characterized in that: The sampling tube sealing valve has a sampling valve chamber and a pressure chamber inside. An elastic diaphragm is fixedly installed between the sampling valve chamber and the pressure chamber. A valve chamber partition for isolating the valve inlet and outlet is installed inside the sampling valve chamber. A sampling valve hole is opened on the valve chamber partition. A sampling valve core is sealed and snapped into the sampling valve hole. A sampling valve rod is fixedly installed at the top of the sampling valve core. The top of the sampling valve rod is fixedly connected to the middle of the elastic diaphragm. A flow guide tube is connected to one end of the sampling tube sealing valve that is connected to the circulation detection tube. One end of the flow guide tube is connected to the pressure chamber. A flow regulating valve is also connected to the flow guide tube.

7. The detection structure according to claim 6, characterized in that: The upper end of the sampling valve rod extends upward into the interior of the pressure chamber, and a limit block is fixedly installed at the top of the sampling valve rod, with a limit hole provided on the limit block; The pressure chamber is fixedly installed with a limiting cylinder that slides into the upper end of the sampling valve rod. The limiting cylinder is provided with a telescopic groove. A slider is slidably fitted inside the telescopic groove. A return spring is installed on the inner end of the slider. A limiting post that can slide into the limiting hole is fixedly installed on the outer end of the slider.

8. The detection structure according to claim 5, characterized in that: The drain pipe sealing valve has a drain valve chamber inside. A drain baffle plate for separating the two ends of the valve is fixedly installed inside the drain valve chamber. A drain valve hole is opened on the drain baffle plate. A drain valve core is sealed and fitted inside the drain valve hole. A drain valve rod is fixedly installed at the top of the drain valve core. A limiting diaphragm fixedly connected to the drain valve rod is installed at the upper end of the drain valve chamber. A spring seat that slides into the top of the drain valve rod is fixedly installed at the top of the drain valve chamber. A pressure spring that abuts against the top of the drain valve rod is installed inside the spring seat.

9. A detection method using the detection structure according to any one of claims 1-8, characterized in that, The specific steps are as follows: First, adjust the feed sealing valve group to the open state and the cleaning sealing valve group to the closed state. Then, drive the culture medium in the bionic simulated organ culture tank to circulate in the circulation detection tube through the hydraulic pumping unit. At this time, the acid-base detection sensor can continuously detect the acid-base of the culture medium flowing through the circulation detection tube. Once the pH test is complete, the cleaning sealing valve assembly is opened, allowing the cleaning components to begin supplying cleaning fluid into the circulating detection tube to clean the circulating detection tube and the detection probe of the pH sensor. Meanwhile, the feed sealing valve assembly is closed after a delay, allowing any residual culture medium in the circulating detection tube to flow back into the biomimetic organ culture tank under the pressure of the cleaning fluid.

10. A biomimetic simulated organ culture vessel, comprising the detection structure described in any one of claims 1-8, characterized in that: It also includes a control module, a temperature regulation unit, a pH regulation unit, a stirring unit, and an oxygen supply unit. The control module intelligently regulates the detection structure and each unit to make the biomimetic organ culture tank simulate the ecological environment of a bovine gallbladder. The stirring unit and the oxygen supply unit keep the culture medium in the biomimetic organ culture tank in a state of agitation through stirring and oxygen input. The pH regulation unit can deliver acidic or alkaline regulating solution into the biomimetic organ culture tank according to the pH of the culture medium detected by the detection structure to adjust the pH value in the biomimetic organ culture tank. The temperature regulation unit can detect and adjust the reaction temperature in the biomimetic organ culture tank online.

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