Tissue culture device for botany experiment

Abstract

The invention relates to the technical field of plant tissue culture, in particular to a botanical experiment tissue culture device which comprises a waste liquid collecting circular groove, a circular protective shell is fixedly connected to the top surface of the waste liquid collecting circular groove, an annular sliding groove is fixedly connected to the inner wall of the circular protective shell, and a placing circular plate is slidably connected to the circular protective shell through the annular sliding groove; the top surface of the placing circular plate is fixedly connected with three incubators arranged in a circumferential array form, the middle of the top surface of the placing circular plate is rotatably connected with a driving rod, the top end of the driving rod is coaxially and fixedly connected with a reciprocating threaded rod, the two ends of the reciprocating threaded rod are coaxially and fixedly connected with baffles, and the top end of the reciprocating threaded rod is coaxially and fixedly connected with a rotating rod; and the other end of the rotating rod is rotationally connected to the inner top surface of the circular protective shell. According to the tissue culture device for botanical experiments, culture device cleaning and automatic culture liquid adding can be completed, and the risk that cultures are exposed to unclean air is reduced due to the fact that the whole process is completed in the device.

Description

Technical Field

[0001] This invention relates to the field of plant tissue culture technology, specifically to a tissue culture apparatus for botanical experiments. Background Technology

[0002] Plant tissue culture technology, as a core experimental method in modern plant biology, plays an irreplaceable role in germplasm resource preservation, rapid propagation of superior varieties, genetic transformation, and secondary metabolite production. Depending on the culture method, it can be divided into solid-state culture and liquid-state culture. Liquid-state culture, in particular, allows explants or cell clusters to fully contact the culture medium, resulting in more uniform nutrient absorption and higher mass transfer efficiency. This facilitates large-scale suspension culture and bioreactor applications, making it an important method for cell-level or small-particle tissue research and production.

[0003] However, during the cultivation process, the nutrients in the culture medium are gradually depleted, and inhibitory substances such as phenols produced by explant metabolism accumulate continuously. Therefore, it is essential to regularly replace the culture medium with fresh material to maintain the optimal environment for tissue growth. Current techniques typically require completely opening or removing the culture container from the culture environment, then aseptically aspirating the old medium and adding fresh medium. This process is not only cumbersome and demands a high level of operator skill, but more importantly, it significantly increases the risk of the culture being exposed to unclean air. Even when performed in a laminar flow hood, actions such as opening the container, pouring, and pipetting can instantly disrupt the sterile environment within the container.

[0004] To address the aforementioned issues, we propose an improvement: a tissue culture apparatus for botanical experiments. Summary of the Invention

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution:

[0006] This invention provides a tissue culture device for plant experiments, including a waste liquid collection circular trough, a circular protective shell fixedly connected to the top surface of the waste liquid collection circular trough, an annular sliding groove fixedly connected to the inner wall of the circular protective shell, a placement circular plate slidably connected to the circular protective shell through the annular sliding groove, and three culture vessels arranged in a circumferential array fixedly connected to the top surface of the placement circular plate.

[0007] A drive rod is rotatably connected to the center of the top surface of the circular plate. A reciprocating threaded rod is coaxially fixed to the top end of the drive rod. Baffles are coaxially fixed to both ends of the reciprocating threaded rod. A rotating rod is coaxially fixed to the top end of the reciprocating threaded rod. The other end of the rotating rod is rotatably connected to the inner top surface of the circular protective shell. A Y-shaped plate is threaded onto the reciprocating threaded rod. A fixing rod, a water supply pipe, and an infusion pipe are sequentially provided on the bottom surface of the three support arms of the Y-shaped plate.

[0008] The top surface of the Y-shaped plate is coaxially fixedly connected to an inner annular slide bar and an outer annular slide bar. An annular plate is slidably connected to the inner annular slide bar, and a residual gear is slidably connected to the outer annular slide bar. The residual gear meshes with a spur gear. The water supply pipe extends through to the outer wall above the Y-shaped plate and is coaxially fixedly connected to the spur gear. An abutment plate is slidably connected to the outer wall of the annular plate through a slide groove. A spring is fixedly connected between the abutment plate and the inner wall of the slide groove. A groove is provided on the side of the abutment plate. One of the support arms of the Y-shaped plate is fixedly connected to an arc-shaped plate with a chamfer on one side through a connecting rod on its top surface.

[0009] As a preferred embodiment of the present invention, the fixing rod and the infusion tube are fixedly connected to the bottom surfaces of two of the support arms of the Y-shaped plate, the water infusion tube is rotatably connected to the bottom surface of the other support arm of the Y-shaped plate, the top end of the water infusion tube is connected to the telescopic water pipe through a sealed rotary joint, the top end of the infusion tube is connected to the telescopic water pipe, and both the water infusion tube and the infusion tube are equipped with wireless control valves.

[0010] The bottom end of the fixed rod is coaxially fixedly connected to a lifting disc with a number of evenly spaced holes, and the bottom end of the water supply pipe is connected to a water nozzle.

[0011] A mounting circular plate is coaxially provided at the top center of the waste liquid collection circular tank. Several support rods are fixedly connected between the bottom surface of the mounting circular plate and the bottom surface of the waste liquid collection circular tank. An annular channel is formed between the inner side of the top of the waste liquid collection circular tank and the outer side of the mounting circular plate.

[0012] At the center of the bottom surface of each culture vessel is a drain pipe equipped with a wirelessly controlled valve. The bottom end of the drain pipe extends through the circular plate and annular channel into the interior of the waste liquid collection trough.

[0013] As a preferred technical solution of the present invention, a geared motor is mounted on the top surface of the mounting circular plate, and a fully driven gear and a partially driven gear are coaxially rotatably connected on the output shaft of the geared motor. The top surfaces of the fully driven gear and the partially driven gear are respectively provided with an anti-forward rotation structure and an anti-reverse rotation structure.

[0014] A driven gear one is coaxially fixedly connected at the center of the bottom surface of the circular plate. A driven gear two is coaxially fixedly connected through the end of the circular plate and the driven gear one. The driven gear one can mesh with the driving incomplete gear, and the driven gear two meshes with the driving complete gear.

[0015] As a preferred technical solution of the present invention, the anti-rotation structure includes a spring rod and a mounting groove coaxially formed on the top surface of the driven gear. A plurality of right-angled triangular blocks are provided on the inner wall of the mounting groove.

[0016] The spring rod is fixedly connected to the outer wall of the output shaft of the geared motor, and the movable end of the spring rod has a chamfer that matches the hypotenuse of the right-angled triangular block.

[0017] As a preferred technical solution of the present invention, the anti-reverse structure includes a spring rod II and a mounting groove II coaxially opened on the top surface of the driven gear II, and a plurality of right-angled triangular blocks II are provided on the inner wall of the mounting groove II.

[0018] The second spring rod is fixedly connected to the outer wall of the output shaft of the geared motor, and the movable end of the second spring rod has a chamfer that matches the hypotenuse of the right-angled triangular block.

[0019] As a preferred embodiment of the present invention, a limiting slide bar is vertically fixedly connected to the outer wall of the rotating rod, and a driving disk is vertically slidably connected to the rotating rod through the limiting slide bar. Several connecting plates are symmetrically fixedly connected between the driving disk and the annular plate.

[0020] As a preferred embodiment of the present invention, a plurality of positioning plates are fixedly connected to the top surface of the outer annular slide bar, and an elastic rope is fixedly connected to the outermost positioning plate. One end of the elastic rope passes through the other plurality of positioning plates and is fixedly connected to the side of the residual gear.

[0021] As a preferred embodiment of the present invention, a telescopic limiting rod is fixedly connected between the top surface of one of the support arms of the Y-shaped plate and the inner top surface of the circular protective shell.

[0022] The beneficial effects of this invention are as follows: This tissue culture device for botanical experiments rotates clockwise using a geared motor to move the culture vessel, which has just discharged the culture liquid, to below the water nozzle. Then, the geared motor rotates counterclockwise, driving the Y-shaped plate downwards via a reciprocating threaded rod. This moves the lifting disc and the water nozzle into the two culture vessels respectively, and moves the infusion tube above another culture vessel, thereby completing the cleaning of the culture vessel and automatically adding the culture liquid. The entire process is completed inside the device, reducing the risk of the culture being exposed to unclean air. Attached Figure Description

[0023] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0024] Figure 1 This is a perspective view of a tissue culture apparatus for botanical experiments according to the present invention;

[0025] Figure 2 This is a top view of a tissue culture apparatus for botanical experiments according to the present invention;

[0026] Figure 3 This invention relates to a tissue culture apparatus for botanical experiments. Figure 2 Sectional view at point AA;

[0027] Figure 4 This is a perspective view of the hidden circular protective shell of a tissue culture device for botanical experiments according to the present invention;

[0028] Figure 5 This is a three-dimensional view of the internal structure of a tissue culture device for botanical experiments according to the present invention.

[0029] Figure 6 This is a top view of the hidden circular protective shell of a tissue culture device for botanical experiments according to the present invention;

[0030] Figure 7 This is a schematic diagram of the water spray nozzle of a tissue culture device for botanical experiments according to the present invention;

[0031] Figure 8 This is a three-dimensional view of the anti-reverse and anti-conversion structures of a tissue culture device for botanical experiments according to the present invention;

[0032] Figure 9 This invention relates to a tissue culture apparatus for botanical experiments. Figure 4 Enlarged view of point A in the image;

[0033] Figure 10 This invention relates to a tissue culture apparatus for botanical experiments. Figure 6 Enlarged view of point B in the image;

[0034] Figure 11 This invention relates to a tissue culture apparatus for botanical experiments. Figure 6 Enlarged view of point C in the image;

[0035] In the diagram: 1. Waste liquid collection trough; 2. Circular protective shell; 3. Placement plate; 4. Incubator; 5. Drive rod; 6. Reciprocating threaded rod; 7. Rotating rod; 8. Y-shaped plate; 9. Fixing rod; 10. Water supply pipe; 11. Infusion pipe; 12. Lifting disc; 13. Water nozzle; 14. Inner annular slide bar; 15. Outer annular slide bar; 16. Circular plate; 17. Residual gear; 18. Spur gear; 19. Contact plate; 20. Arc-shaped plate; 21. Limiting slide bar; 22. Drive disc; 23. Connecting plate; 24. Mounting disc; 25. Gear motor; 26. Fully driven gear; 27. Partially driven gear; 28. Driven gear one; 29. ​​Positioning plate; 30. Driven gear two; 31. Spring rod one; 32. Mounting groove one; 33. Right-angled triangular block one; 34. Mounting groove two; 35. Spring rod two; 36. Right-angled triangular block two; 37. Elastic rope. Detailed Implementation

[0036] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0037] Example: Figures 1-11 As shown, a tissue culture device for a plant experiment includes a waste liquid collection trough 1, a circular protective shell 2 fixedly connected to the top surface of the waste liquid collection trough 1, a sealed door installed on the outer wall of the circular protective shell 2, and an existing ultraviolet disinfection lamp, supplemental light lamp and temperature and humidity controller installed inside it.

[0038] The inner wall of the circular protective shell 2 is fixedly connected with an annular groove, and the circular protective shell 2 is slidably connected to a circular plate 3 through the annular groove. The top surface of the circular plate 3 is fixedly connected to three incubators 4 arranged in a circular array.

[0039] A drive rod 5 is rotatably connected to the top center of the circular plate 3. A reciprocating threaded rod 6 is coaxially fixedly connected to the top end of the drive rod 5. Baffles are coaxially fixedly connected to both ends of the reciprocating threaded rod 6. A rotating rod 7 is coaxially fixedly connected to the top end of the reciprocating threaded rod 6. The other end of the rotating rod 7 is rotatably connected to the inner top surface of the circular protective shell 2. A Y-shaped plate 8 is threadedly connected to the reciprocating threaded rod 6. A fixing rod 9, a water supply pipe 10, and an infusion pipe 11 are sequentially provided on the bottom surface of the three support arms of the Y-shaped plate 8.

[0040] The top surface of the Y-shaped plate 8 is coaxially fixedly connected to an inner annular slide bar 14 and an outer annular slide bar 15. An annular plate 16 is slidably connected to the inner annular slide bar 14, and a residual gear 17 is slidably connected to the outer annular slide bar 15. The residual gear 17 meshes with a spur gear 18. The water supply pipe 10 extends through to the outer wall above the Y-shaped plate 8 and is coaxially fixedly connected to the spur gear 18. A contact plate 19 is slidably connected to the outer wall of the annular plate 16 through a slide groove. A spring is fixedly connected between the contact plate 19 and the inner wall of the slide groove. A groove is provided on the side of the contact plate 19. One of the support arms of the Y-shaped plate 8 is fixedly connected to an arc-shaped plate 20 with a chamfer on one side through a connecting rod.

[0041] like Figure 5 and Figure 9 As shown, during each clockwise rotation of the rotating rod 7, the contact plate 19 first contacts the side of the residual gear 17, and then the contact plate 19 rotates clockwise with the residual gear 17. During this process, the elastic rope 37 is stretched. When the water nozzle 13 is fully inserted into the incubator 4, the reduction motor 25 stops running. When the contact plate 19 passes the arc plate 20, the contact plate 19 is pressed into the groove of the annular plate 16 by the contact between the chamfer on the arc plate 20 and the groove on the contact plate 19. After the contact plate 19 is no longer restricted, the residual gear 17 is reset under the action of the elastic rope 37. During the reset process of the residual gear 17, the residual gear 17 drives the spur gear 18 to rotate, and the spur gear 18 drives the water pipe 10 to rotate.

[0042] At the same time, the external pump is started, and water is sprayed out through the water outlet nozzle 13 to rinse the inner wall of the incubator 4. During this process, the wireless control valve on the drain pipe of the incubator 4 located below the water outlet nozzle 13 is in the open state.

[0043] The fixed rod 9 and the infusion tube 11 are fixedly connected to the bottom surface of two of the support arms of the Y-shaped plate 8. The water infusion tube 10 is rotatably connected to the bottom surface of the other support arm of the Y-shaped plate 8. The top end of the water infusion tube 10 is connected to the telescopic water pipe through a sealed rotary joint. The top end of the infusion tube 11 is connected to the telescopic water pipe. Both the water infusion tube 10 and the infusion tube 11 are equipped with wireless control valves. It should be noted that the three support arms of the Y-shaped plate 8 are arranged in a circular array.

[0044] The bottom end of the fixed rod 9 is coaxially fixedly connected to a lifting disc 12 with a number of evenly spaced holes, and the bottom end of the water supply pipe 10 is connected to a water outlet nozzle 13.

[0045] A mounting plate 24 is coaxially provided at the top center of the waste liquid collection tank 1. Several support rods are fixedly connected between the bottom surface of the mounting plate 24 and the inner bottom surface of the waste liquid collection tank 1. An annular channel is formed between the inner side of the top of the waste liquid collection tank 1 and the outer side of the mounting plate 24.

[0046] At the center of the bottom surface of each incubator 4, there is a drain pipe with a wireless control valve. The bottom end of the drain pipe passes through the circular plate 3 and the annular channel and extends into the interior of the waste liquid collection tank 1. In addition, the waste liquid collection tank 1 is connected to an external outlet pipe.

[0047] A geared motor 25 is mounted on the top surface of the mounting plate 24. A fully driven gear 26 and a partially driven gear 27 are coaxially rotatably connected on the output shaft of the geared motor 25. The top surfaces of the fully driven gear 26 and the partially driven gear 27 are respectively provided with an anti-forward rotation structure and an anti-reverse rotation structure.

[0048] A driven gear 28 is coaxially fixedly connected at the center of the bottom surface of the circular plate 3. A driven gear 30 is coaxially fixedly connected through the end of the circular plate 3 and the driven gear 28. The driven gear 28 can mesh with the driving incomplete gear 27, and the driven gear 30 meshes with the driving complete gear 26.

[0049] The anti-rotation structure includes a spring rod 31 and a mounting groove 32 coaxially opened on the top surface of the driven gear 28. Several right-angled triangular blocks 33 are provided on the inner wall of the mounting groove 32.

[0050] Spring rod 31 is fixedly connected to the outer wall of the output shaft of geared motor 25. The movable end of spring rod 31 is provided with a chamfer that matches the hypotenuse of right-angled triangular block 33.

[0051] like Figure 8 As shown, when the output shaft of the geared motor 25 rotates clockwise, the chamfer of the movable end of the spring rod 31 will be retracted into the fixed end of the spring rod 31 under the contact of the hypotenuse of the right-angled triangular block 33. Thus, the geared motor 25 cannot drive the incomplete drive gear 27 to rotate clockwise. Conversely, if the geared motor 25 rotates counterclockwise, the other side of the movable end of the spring rod 31 will contact the straight side of the right-angled triangular block 33, thereby driving the incomplete drive gear 27 to rotate counterclockwise synchronously.

[0052] The anti-reverse structure includes a spring rod 35 and a mounting groove 34 coaxially opened on the top surface of the driven gear 30. Several right-angled triangular blocks 36 are provided on the inner wall of the mounting groove 34.

[0053] Spring rod 2 35 is fixedly connected to the outer wall of the output shaft of the geared motor 25. The movable end of spring rod 2 35 is provided with a chamfer that matches the hypotenuse of right-angled triangular block 2 36.

[0054] like Figure 8 As shown, when the output shaft of the geared motor 25 rotates counterclockwise, the chamfer of the movable end of the spring rod 35 will be retracted into the fixed end of the spring rod 35 under the contact of the hypotenuse of the right-angled triangular block 36. Thus, the geared motor 25 cannot drive the drive gear 26 to rotate counterclockwise. Conversely, if the geared motor 25 rotates clockwise, the other side of the movable end of the spring rod 35 will contact the straight side of the right-angled triangular block 36, thereby driving the drive gear 26 to rotate clockwise synchronously.

[0055] A limiting slide bar 21 is vertically fixed to the outer wall of the rotating rod 7. A driving disc 22 is vertically slidably connected to the rotating rod 7 via the limiting slide bar 21. Several connecting plates 23 are symmetrically fixed between the driving disc 22 and the annular plate 16. Figure 3 and Figure 4 As shown, the function of the limiting slide bar 21 is to prevent the rotating rod 7 from rotating relative to the driving disk 22.

[0056] Several positioning plates 29 are fixedly connected to the top surface of the outer annular slide bar 15. An elastic rope 37 is fixedly connected to the outermost positioning plate 29. One end of the elastic rope 37 passes through the other positioning plates 29 and is fixedly connected to the side of the residual gear 17. The function of the elastic rope 37 is to return the residual gear 17 to its original position.

[0057] like Figure 3 As shown, a telescopic limiting rod is fixedly connected between the top surface of one of the support arms of the Y-shaped plate 8 and the inner top surface of the circular protective shell 2. The telescopic limiting rod limits the up and down movement of the Y-shaped plate 8.

[0058] First and foremost, it should be noted that all of the aforementioned electrical components are electrically connected to an external controller, such as... Figure 4 , Figure 5 and Figure 8 As shown, when the liquid culture medium needs to be replaced, the geared motor 25 is started by the external controller. The geared motor 25 rotates counterclockwise. At this time, the geared motor 25 drives the active gear 26 to rotate counterclockwise through the anti-clockwise rotation structure. The active gear 26 drives the driven gear 30 to rotate counterclockwise. The driven gear 30 drives the drive rod 5 to rotate, which in turn drives the reciprocating threaded rod 6 to rotate. The reciprocating threaded rod 6 drives the Y-shaped plate 8 to move upward along the telescopic limit rod. The Y-shaped plate 8 separates the plant tissue from the culture vessel 4 through the fixing rod 9 and the lifting disc 12. Then the geared motor 25 is turned off. The wireless control valve on the drain pipe at the bottom of the culture vessel 4 is opened by the external controller to drain the old culture liquid.

[0059] Next, the geared motor 25 is started by the external controller. The geared motor 25 rotates clockwise. At this time, the geared motor 25 drives the active incomplete gear 27. Each rotation of the active incomplete gear 27 will drive the driven gear 28 to rotate one-third of a rotation, thereby rotating the culture vessel 4, which has just discharged the culture liquid, to below the water outlet nozzle 13. Then, the geared motor 25 is started to rotate counterclockwise. The reciprocating threaded rod 6 drives the Y-shaped plate 8 to move down. The fixing rod 9 puts the lifting disc 12 into the new culture vessel 4. The wireless control valve on the water supply pipe 10 is opened. The external pump delivers clean water to the water outlet nozzle 13 through the water supply pipe 10 to further rinse the culture vessel 4. When the culture vessel 4 needs to be replaced, the culture liquid is delivered to the cleaned culture vessel 4 through the infusion pipe 11. At this time, the wireless control valve on the drain pipe of the culture vessel 4 below the infusion pipe 11 is in the closed state.

[0060] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A tissue culture apparatus for botanical experiments, comprising a waste liquid collection trough (1), characterized in that, The top surface of the waste liquid collection trough (1) is fixedly connected to a circular protective shell (2), the inner wall of the circular protective shell (2) is fixedly connected to an annular sliding groove, the circular protective shell (2) is slidably connected to a placement circular plate (3) through the annular sliding groove, and the top surface of the placement circular plate (3) is fixedly connected to three incubators (4) arranged in a circular array. A drive rod (5) is rotatably connected to the top center of the circular plate (3). A reciprocating threaded rod (6) is coaxially fixed to the top end of the drive rod (5). Baffles are coaxially fixed to both ends of the reciprocating threaded rod (6). A rotating rod (7) is coaxially fixed to the top end of the reciprocating threaded rod (6). The other end of the rotating rod (7) is rotatably connected to the inner top surface of the circular protective shell (2). A Y-shaped plate (8) is threaded onto the reciprocating threaded rod (6). A fixing rod (9), a water pipe (10), and an infusion pipe (11) are sequentially provided on the bottom surface of the three arms of the Y-shaped plate (8). The top surface of the Y-shaped plate (8) is coaxially fixedly connected to an inner annular slide bar (14) and an outer annular slide bar (15). An annular plate (16) is slidably connected to the inner annular slide bar (14), and a residual gear (17) is slidably connected to the outer annular slide bar (15). The residual gear (17) meshes with a spur gear (18). The water pipe (10) extends through to the outer wall above the Y-shaped plate (8) and is coaxially fixedly connected to the spur gear (18). A chamfered abutment plate (19) is slidably connected to the outer wall of the annular plate (16) through a slide groove. A spring is fixedly connected between the abutment plate (19) and the inner wall of the slide groove. A groove is provided on the side of the abutment plate (19). One of the support arms of the Y-shaped plate (8) is fixedly connected to an arc-shaped plate (20) with chamfered sides through a connecting rod.

2. The tissue culture apparatus for botanical experiments according to claim 1, characterized in that, The fixed rod (9) and the infusion tube (11) are fixedly connected to the bottom surfaces of two of the support arms of the Y-shaped plate (8). The water infusion tube (10) is rotatably connected to the bottom surface of the other support arm of the Y-shaped plate (8). The top end of the water infusion tube (10) is connected to the telescopic water pipe through a sealed rotary joint. The top end of the infusion tube (11) is connected to the telescopic water pipe. Both the water infusion tube (10) and the infusion tube (11) are equipped with wireless control valves. The bottom end of the fixed rod (9) is coaxially fixedly connected to a lifting disc (12) with a plurality of evenly spaced holes, and the bottom end of the water supply pipe (10) is connected to a water outlet nozzle (13).

3. The tissue culture apparatus for botanical experiments according to claim 1, characterized in that, The waste liquid collection trough (1) is provided with a mounting plate (24) coaxially at the top center. Several support rods are fixedly connected between the bottom surface of the mounting plate (24) and the bottom surface of the waste liquid collection trough (1). An annular channel is formed between the inner side of the top of the waste liquid collection trough (1) and the outer side of the mounting plate (24). Each of the culture vessels (4) has a drain pipe with a wireless control valve connected to the center of its bottom surface. The bottom end of the drain pipe extends through the circular plate (3) and the annular channel into the interior of the waste liquid collection trough (1).

4. The tissue culture apparatus for botanical experiments according to claim 3, characterized in that, A geared motor (25) is mounted on the top surface of the mounting plate (24). A fully driven gear (26) and a partially driven gear (27) are coaxially rotatably connected on the output shaft of the geared motor (25). The top surfaces of the fully driven gear (26) and the partially driven gear (27) are respectively provided with an anti-forward rotation structure and an anti-reverse rotation structure. A driven gear 1 (28) is coaxially fixedly connected at the center of the bottom surface of the placement circular plate (3). A driven gear 2 (30) is coaxially fixedly connected through the end of the placement circular plate (3) and the driven gear 1 (28). The driven gear 1 (28) can mesh with the driving incomplete gear (27), and the driven gear 2 (30) meshes with the driving complete gear (26).

5. The tissue culture apparatus for botanical experiments according to claim 4, characterized in that, The anti-rotation structure includes a spring rod (31) and a mounting groove (32) coaxially opened on the top surface of the driven gear (28). Several right-angled triangular blocks (33) are provided on the inner wall of the mounting groove (32). The spring rod (31) is fixedly connected to the outer wall of the output shaft of the geared motor (25), and the movable end of the spring rod (31) is provided with a chamfer that cooperates with the hypotenuse of the right-angled triangular block (33).

6. The tissue culture apparatus for botanical experiments according to claim 5, characterized in that, The anti-reverse structure includes a spring rod (35) and a mounting groove (34) coaxially opened on the top surface of the driven gear (30). Several right-angled triangular blocks (36) are provided on the inner wall of the mounting groove (34). The second spring rod (35) is fixedly connected to the outer wall of the output shaft of the geared motor (25), and the movable end of the second spring rod (35) is provided with a chamfer that cooperates with the hypotenuse of the right-angled triangular block (36).

7. The tissue culture apparatus for botanical experiments according to claim 5, characterized in that, A limiting slide bar (21) is vertically fixedly connected to the outer wall of the rotating rod (7). A driving disc (22) is vertically slidably connected to the rotating rod (7) through the limiting slide bar (21). Several connecting plates (23) are symmetrically fixedly connected between the driving disc (22) and the annular plate (16).

8. The tissue culture apparatus for botanical experiments according to claim 1, characterized in that, The top surface of the outer annular slide bar (15) is fixedly connected to several positioning plates (29). An elastic rope (37) is fixedly connected to the outermost positioning plate (29). One end of the elastic rope (37) passes through the other positioning plates (29) and is fixedly connected to the side of the residual gear (17).

9. A tissue culture apparatus for botanical experiments according to claim 1, characterized in that, A telescopic limiting rod is fixedly connected between the top surface of one of the support arms of the Y-shaped plate (8) and the inner top surface of the circular protective shell (2).

Images