Method for screening good growth and disease-resistant characters of carassius auratus gibelio

Abstract

The invention provides a method for screening good growth and disease-resistant characters of carassius auratus gibelio, and belongs to the technical field of fish culture. According to the method, the ratio of albumin to globulin in the blood of the carassius auratus gibelio serves as a screening standard, and if the ratio of albumin (A) to globulin (G) is larger than or equal to 0.75, it is judged that an individual has excellent growth and disease resistance. By measuring the ratio of the allogynogenetic prussian carp blood albumin (A) to the globulin (G), the allogynogenetic prussian carp individuals with excellent growth performance and disease resistance can be screened out, and a technical support is provided for screening the allogynogenetic prussian carp with excellent characters.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of fish farming, in particular to a screening method for growth and disease resistance of Carassius auratus gibelio. BACKGROUND

[0002] Carassius auratus gibelio is a kind of offspring which is cultivated by using natural gynogenesis, taking square silver white female as mother and Xingguo red carp as father. It has good heterosis, obvious yield increasing effect, tender meat, rich nutrition, long water survival time, wide food habit, strong resistance, easy feeding, strong adaptability, high survival rate, fast growth, large individual, short feeding cycle and other characteristics, and shows good economic traits in breeding production. Carassius auratus gibelio is one of China's major freshwater fish, and its economic benefits are very significant, and it is welcomed by breeders and consumers. More than 30 provinces, cities and autonomous regions in China have introduced and bred Carassius auratus gibelio, and the annual breeding yield of Carassius auratus gibelio is about 3 million tons, which has become the main variety of pond and net cage breeding. However, with the continuous expansion of breeding scale, more and more diseases occur in breeding, which restricts the breeding of the variety. The outbreak of hemorrhagic disease is a kind of infectious disease that is popular and harmful in recent years in artificial breeding of Carassius auratus gibelio. It has been confirmed that the pathogen of the disease is Aeromonas hydrophila. The clinical symptoms are that the diseased fish floats alone, has poor activity, reacts slowly when frightened, and has decreased or stopped feeding. The mouth, upper and lower jaws, eye sockets, gill covers and fin bars of the diseased fish have different degrees of hyperemia, and the lesion site is red or purple. The hemorrhagic disease mainly harms fish bodies above one year old, and the epidemic season is generally from April to October, and the incidence peaks from June to September. Under the conditions of high density and intensive breeding (especially in professional fishing ponds), the disease occurs frequently, it is contagious, and the mortality rate is high (about 80%). Usually, breeders use strong chlorine and bleaching powder for disinfection, and take antibiotics for treatment. In addition, the red skin disease caused by injury of fish during stocking and infection of body surface parasites also affects the quality of Carassius auratus gibelio. Therefore, under the conditions of factoryization and high density breeding, it is of positive significance to select Carassius auratus gibelio with fast growth and strong immunity in breeding production. SUMMARY

[0003] Therefore, the present application provides a screening method for growth and disease resistance of Carassius auratus gibelio to solve the above problems.

[0004] In order to achieve the above application purposes, the present application provides the following technical solutions:

[0005] The application provides a screening method for growth and disease resistance of gynogenetic gibel carp, characterized by taking the ratio of albumin and globulin in blood of the gynogenetic gibel carp as a screening standard, and if the ratio of albumin and globulin is greater than or equal to 0.75, the individual is determined to have excellent growth and disease resistance.

[0006] Preferably, the gynogenetic gibel carp is one-year-old or two-year-old gynogenetic gibel carp.

[0007] Preferably, the blood of the gynogenetic gibel carp is taken from the main blood vessel of the caudal peduncle.

[0008] Preferably, the gynogenetic gibel carp is triploid or tetraploid.

[0009] By adopting the above technical scheme, the application has the following beneficial effects: the application takes the ratio of albumin (A) and globulin (G) in blood of the gynogenetic gibel carp as a screening standard, and if the ratio of albumin and globulin is greater than or equal to 0.75, the individual is determined to have excellent growth and disease resistance. The application can screen out gynogenetic gibel carp individuals with excellent growth performance and disease resistance by determining the ratio of albumin (A) and globulin (G) in blood of the gynogenetic gibel carp, thereby providing technical support for screening gynogenetic gibel carp with excellent traits. BRIEF DESCRIPTION OF DRAWINGS

[0010] Figure 1 Table 1 and Table 2 show the body weight and A / G of the gynogenetic gibel carp in Example 1.

[0011] Figure 2 Table 1 and Table 2 show the body weight and A / G of the gynogenetic gibel carp in Example 1.

[0012] Figure 3 Table 3 shows the IFN-γ gene expression of the gynogenetic gibel carp with high A / G and the gynogenetic gibel carp with low A / G. DETAILED DESCRIPTION

[0013] The technical solutions provided by the application will be described in detail below in combination with examples, but they should not be understood as limiting the scope of protection of the application.

[0014] Example 1

[0015] In June 2023, 28 gynogenetic gibel carp "Zhongke No. 3" of 60 days old and with the same size were selected for pond culture, and the water temperature during the culture was 28-32℃. The gynogenetic gibel carp was cultured to 180 days old, and the ratio of albumin (A) and globulin (G) in blood and the body weight of the 28 gynogenetic gibel carp were determined. The results are shown in Table 1 and Table 2. Figure 1 Linear regression analysis was performed by using SPSS software, and the body weight and A / G had significant correlation, p=0.0004.

[0016] Table 1 A / G and body weight of one-year-old gynogenetic gibel carp

[0017] Table 1 A / G and body weight of one-year-old gynogenetic gibel carp

[0018]

[0019] Example 2

[0020] In September 2024, 101 Carassius auratus gibelio "Zhongke 3" were randomly selected for caudal fin blood sampling, and the detection results are shown in Table 2. Using SPSS statistical software for correlation analysis, the weight and A / G have significant correlation, p<0.0001.

[0021] Table 2 A / G and weight of one-year-old Carassius auratus gibelio

[0022]

[0023]

[0024] Six Carassius auratus gibelio with A / G≥0.75 and six individuals with A / G<0.75 were selected for electronic tag injection, so as to facilitate the differentiation of each individual in the later stage. Cyhv-2 virus culture solution (LD50=100 μL / 100g) was injected into the abdominal cavity, and 100 three days later, five experimental fish spleen tissues were taken, total RNA was extracted, and IFN-γ gene expression was detected, and the results are shown in Figure 3 The IFN-γ gene expression of the high A / G group is significantly higher than that of the low A / G group. IFN-γ (interferon-γ) is an important immune indicator, which indicates that the disease resistance of Carassius auratus gibelio with A / G≥0.75 is higher than that of individuals with A / G<0.75.

[0025] From the above examples, it can be seen that the present application provides a screening method for excellent growth and disease resistance traits of Carassius auratus gibelio. By determining the ratio of albumin (A) and globulin (G) in the blood of Carassius auratus gibelio, the present application can screen out Carassius auratus gibelio individuals with excellent growth and disease resistance performance.

[0026] The above only describes the preferred embodiments of the present application, and it should be noted that for ordinary skilled persons in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A method for screening superior traits of growth and disease resistance in heterozygous silver carp, characterized in that, The ratio of albumin to globulin in the blood of heterozygous silver carp was used as the screening criterion. If the albumin to globulin ratio was ≥ A score of 0.75 indicates that the individual possesses excellent growth and disease resistance.

2. The screening method according to claim 1, characterized in that, The heterotrophic silver carp mentioned are one-year-old or two-year-old heterotrophic silver carp.

3. The screening method according to claim 1, characterized in that, The blood of heterotrophic silver carp is taken from the main blood vessel of the caudal peduncle.

4. The screening method according to claim 1, characterized in that, The heterotrophic silver carp is triploid or tetraploid.

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