Use method of composite probiotic deodorization disinfectant

Abstract

The invention belongs to the technical field of microbial deodorization, and particularly relates to a use method of a compound probiotic deodorization disinfectant. The method comprises the following steps: making change curves of feces discharge and urine discharge of livestock of different day ages; manufacturing excrement discharge amount coefficients and urine discharge amount coefficients of different varieties of livestock; a deodorization ingredient proportioning scheme is formulated, and the deodorization disinfectant with the needed microbial inoculum concentration is prepared; spraying the body surfaces of the livestock and living places of the livestock to ensure that the bacterial liquid uniformly covers a target area to form a biological membrane; and / or adding the deodorization disinfectant into the livestock drinking water, and spraying once every 3-7 days. According to the method, the dosage of the microbial inoculum is adjusted according to the defecation amount and urination amount curves and variety difference coefficients of pigs at different day ages, and an accurate material proportioning scheme is provided, so that the deodorization effect is more accurate and effective. The larger the day age is, the larger the defecation amount and the urination amount are, and the spraying amount is correspondingly increased; the spraying amount of different varieties of pigs is adjusted according to the difference coefficient of the defecation amount and the urination amount, and cost control is accurate.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of microbial deodorization, and particularly relates to a use method of a composite probiotic deodorizing and disinfecting solution. BACKGROUND

[0002] The odor of pig house is mainly ammonia (NH3) and hydrogen sulfide (H2S), and the concentration exceeding the standard will cause respiratory diseases of pigs (such as the incidence of atrophic rhinitis increasing by 20%-40%) and growth retardation (daily weight gain decreasing by 5%-15%). With the development of scale and intensive breeding of livestock and poultry industry, the breeding density is increasing, which leads to the increase of the concentration of harmful gases such as ammonia and hydrogen sulfide in the breeding environment, and seriously affects the health of livestock and poultry and the breeding efficiency. At present, the deodorization of domestic and foreign livestock and poultry farms mainly adopts physical, chemical and microbial methods. The chemical deodorant, represented by sodium hypochlorite and peroxoacetic acid, can quickly neutralize the odor, but it is easy to corrode the equipment, has residual toxicity (such as the irritation rate of chlorine preparation to the respiratory mucosa of pigs reaching 65%), and cannot inhibit the regeneration of odor. The physical adsorption method: the adsorption materials such as activated carbon and zeolite need to be replaced after saturation, and the operation cost is high (the treatment cost of each cubic meter is 8-12 yuan), and the odor generation cannot be reduced from the source. The biological deodorization method relies on specific microbial flora to degrade odor, and gradually becomes a research hotspot and is widely used due to its environmental protection, safety and durability. It has been widely applied to the prevention and control of foul odor due to its high treatment efficiency, low energy consumption, low cost and easy operation.

[0003] The biological deodorization technology mainly utilizes the metabolic activity of microorganisms to decompose or transform odor substances. The biological deodorization technology has incomparable advantages over traditional methods, especially for pollutants with good water solubility, which has good treatment effect and does not produce secondary pollution after treatment, so it has a wide prospect in the treatment of odor. The research work on biological deodorization in China started relatively late, and relevant laboratory research work was carried out only after the 1990s. Therefore, there are still many problems to be solved in theoretical research and practical application.

[0004] Some biological deodorants have been developed on the market, such as single spraying deodorants, which have short effect maintenance time and need to be sprayed frequently (1-2 times a day), increasing the labor cost; some deodorants need to be moved to another place when used, and the environment needs to be sprayed and disinfected, etc. All of these bring inconvenience to use, and there is no strict standard for the dosage, such as how to determine the dosage for different types of pigs, the growth cycle of pigs and the dosage of deodorant, which may greatly cause waste of deodorant or too little dosage to achieve the effect.

[0005] Therefore, there is an urgent need for a safe and convenient deodorization scheme for livestock places and feeding farms, which can accurately control the cost with a standard dosage, maximize the deodorization effect and sterilization, inhibit the growth of harmful bacteria, and has a long duration. SUMMARY

[0006] The technical problem solved by the present application is to provide a use method of a composite probiotic deodorizing disinfectant, determine the weight ratio of Bacillus subtilis and Aspergillus oryzae in the bacterial agent based on different pig excretion and urine output curves and breed difference coefficients, and formulate a personalized deodorizing ingredient ratio scheme, so that the pig house can maximize the deodorizing effect and sterilization without wasting deodorizing disinfectant and accurately controlling the cost.

[0007] The technical scheme adopted is as follows:

[0008] A use method of a composite probiotic deodorizing disinfectant, the use method comprising the following steps:

[0009] (1) Track and count the excretion and urine output of different age poultry, and make excretion and urine output change curves;

[0010] (2) Determine the excretion and urine output coefficients of different breeds of poultry;

[0011] (3) According to the results obtained in steps (1) and (2), formulate a deodorizing ingredient ratio scheme, take the required bacterial agent, and prepare the dosage according to the weight ratio, add pure water, adsorbent, acid-base regulator, nutrient agent and protective agent, mix uniformly, and prepare the deodorizing disinfectant with the required concentration of the bacterial agent;

[0012] (4) Put the deodorizing disinfectant in the spraying equipment, and spray the poultry body surface and its living place to ensure that the bacterial solution uniformly covers the target area and forms a biofilm;

[0013] And / or, add the deodorizing disinfectant to the drinking water of the poultry;

[0014] (5) Spray once every 3-7 days to ensure the deodorizing effect.

[0015] Preferably, the poultry includes any one of pigs, sheep, cattle, horses, rabbits, quails, ducks, chickens and geese.

[0016] Preferably, the poultry is a pig.

[0017] Preferably, in step (1), the pigs are divided into four stages, i.e. 0-28 days, 29-70 days, 71-180 days and more than 180 days, and the excretion and urine output of each pig per day are tracked and counted to obtain the excretion and urine output change curves of different age pigs.

[0018] In step (2), a standard breed of pig is selected according to the physiological characteristics of different breeds of pigs, and the excretion and urine output coefficients of other breeds of pigs are determined based on the standard breed of pig.

[0019] Preferably, the bacterial agent comprises Bacillus subtilis 10-25 parts, Aspergillus oryzae 20-50 parts; the viable bacterial number of Bacillus subtilis and Aspergillus oryzae accounts for 40-60% of the total.

[0020] Preferably, the adsorbent is one or a mixture of activated carbon and zeolite; the acid-base regulator is one or a mixture of sodium bicarbonate and citric acid; the nutrient agent is one or a mixture of glucose and amino acid; the protective agent is one or a mixture of glycerol and alginic acid; and in the deodorizing and disinfecting solution, the adsorbent is 5-15 parts by weight, the acid-base regulator is 1-5 parts by weight, the nutrient agent is 3-10 parts by weight, and the protective agent is 2-8 parts by weight.

[0021] Preferably, the deodorizing and disinfecting solution is prepared by diluting the bacterial agent with water at a dilution ratio of 50-100 times, and the viable bacterial number in the bacterial agent is greater than or equal to 10 8 CFU / mL, and the dilution is uniform.

[0022] Preferably, the spraying amount and interval are determined according to the physiological characteristics of pigs of different breeds, the age of pigs, and the amount of excrement and urine; the spraying amount is increased with the increase of the age of pigs and the amount of excrement and urine.

[0023] Preferably, the weight ratio of Bacillus subtilis and Aspergillus oryzae in the bacterial agent is determined based on the amount of excrement and urine of pigs of different ages and the breed difference coefficient, and a personalized deodorizing and disinfecting solution ratio scheme is formulated.

[0024] Preferably, the pH value of the deodorizing and disinfecting solution is 5.0-6.5, and after spraying, a biofilm can be formed on the surface of the poultry and livestock, which can continuously secrete protease, lipase, amylase and organic acid.

[0025] Compared with the prior art, the present application has the following advantages:

[0026] The present application provides a precise material ratio scheme according to the difference in the amount of excrement and urine of pigs of different ages and the breed difference, and adjusts the amount of bacterial agent according to the amount of excrement and urine of pigs of different ages and the breed difference coefficient, so that the deodorizing effect is more precise and effective.

[0027] The present application precisely determines the scheme to ensure that the bacterial solution uniformly covers the target area and forms a biofilm. The specific amount is adjusted according to the age, breed, amount of excrement and urine of pigs. The spraying amount is increased with the increase of the age of pigs and the amount of excrement and urine; the spraying amount is adjusted according to the difference in the amount of excrement and urine of different breeds of pigs, so as to accurately control the cost.

[0028] The bacillus subtilis used in the application can secrete protease and lipase, can decompose organic matters (such as protein and fat) on the surface of pigs, reduce the generation of odor precursor, and reduce the generation of odor from the source; the aspergillus oryzae can produce amylase, glucoamylase, phytase and organic acid, can degrade carbohydrates and phytic acid, acidify the environment, inhibit the growth of spoilage bacteria, and further reduce the generation of odor. The composite probiotic deodorizing disinfectant liquid of the application combines the synergistic effect of bacillus subtilis and aspergillus oryzae, realizes the whole-chain deodorization of "decomposition-transformation-acidification", and has the advantages of safety, high efficiency, durability and long-lasting deodorization effect. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 A flow chart of the use method of the composite probiotic deodorizing disinfectant liquid.

[0030] Figure 2 A curve graph of the excretion amount and the urine amount of pigs of different ages.

[0031] Figure 3 A difference coefficient graph of the excretion amount and the urine amount of pigs of different breeds.

[0032] Figure 4 A comparison curve graph before and after spraying for pigs of different ages and breeds.

[0033] Figure 5 A comparison graph of the ammonia concentration before and after spraying. DETAILED DESCRIPTION

[0034] The drawings are only used for illustrative purposes; the following detailed description aims to describe the technical solutions described in the application in detail and clearly. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as generally understood by those skilled in the art to which the application belongs.

[0035] If not specified, the raw materials used in the application can be purchased by conventional methods, and the data measured by experiments can also be realized by conventional methods.

[0036] Example 1

[0037] A use method of a composite probiotic deodorizing disinfectant liquid, the use method comprising the following steps:

[0038] (1) Track and count the excretion amount and the urine amount of different ages of poultry and livestock, and make a curve graph of the excretion amount and the urine amount.

[0039] The piglets are divided into four stages, namely 0-28 days, 29-70 days, 71-180 days, and more than 180 days. Through long-term tracking experiments, it is found that the excretion amount and urine output of pigs of different ages are significantly different. The overall trend is that the excretion amount and urine output of milk piglets are less, and gradually increase with age, reaching a peak in the fattening pig period and tending to be stable, providing key basis for material proportioning. The specific data is shown in the excretion amount and urine output curve with age change in Table 1. According to the statistical results in Table 1, the accurate excretion amount and urine output change with age statistics are obtained according to the conventional statistical methodology, and the results are shown in Figure 2

[0040] Table 1 Excretion amount and urine output change statistics with age

[0041] Age stage (days) Fecal output (g / day·head) Urine output (ml / day·head) 0-28 50-100 100-200 29-70 200-400 300-500 71-180 500-800 600-1000 180+ 600-900 800-1200

[0042] Table 2 Tracking experiment to obtain accurate excretion amount and urine output change statistics with age

[0043] Age stage (days) Fecal output (g / day·head) Urine output (ml / day·head) 0-28 75 150 29-70 300 400 71-180 650 800 180+ 750 1000

[0044] (2) Making excretion amount and urine output coefficients of different varieties of poultry and livestock

[0045] Different varieties of pigs have different physiological characteristics, and the excretion amount and urine output also differ. Selecting Landrace as the standard variety, through single factor experiment, selecting 20 pigs of the same variety and batch for testing. Taking the average value (tested according to the method of key laboratory of animal science academy), the excretion amount and urine output difference coefficient of each variety relative to the standard variety is determined, providing key basis for material proportioning. The specific data is shown in Table 3 and Figure 2

[0046] Table 3 Excretion and urine output coefficients of different pigs

[0047] Breed category Fecal output coefficient Urine output coefficient Landrace (standard breed) 1.00 1.00 Large white 1.10 1.05 Duroc 1.20 1.10 Local breed 1.15 1.08

[0048] (3) According to the results obtained in steps (1) and (2), develop a deodorization proportioning scheme.

[0049] Fully considering the influence of physiological characteristics and variety differences of pigs on deodorization effect, it can more accurately meet the deodorization needs of different pig houses, improve the deodorization efficiency, and the details are shown in Table 4 and Figure 3

[0050] Table 4 Excretion amount and urine output curve of pigs of different ages and variety difference coefficient adjustment of bacteria agent dosage

[0051]

[0052] ​​​

[0053] For pigs of different ages and different breeds, the required bacterial agent is prepared according to the weight ratio. The bacterial agent includes Bacillus subtilis 10-25 parts, Aspergillus oryzae 20-50 parts; the viable bacterial count of Bacillus subtilis and Aspergillus oryzae accounts for 40%-60%. Water, adsorbent, acid-base regulator, nutrient agent and protective agent are added and mixed uniformly to prepare the required concentration of deodorizing and disinfecting solution.

[0054] (4) The deodorizing and disinfecting solution is placed in a spraying device. Suitable spraying devices are selected according to the environment of the pig house, such as sprayers, sprinkler systems, etc. Sprayers have the characteristics of flexible operation and low cost, and are suitable for small-scale pig houses or local spraying; the sprinkler system can realize large-area and uniform spraying, and is suitable for large-scale pig houses. According to the size and layout of the pig house, suitable spraying equipment is selected, and the normal operation of the equipment is ensured.

[0055] Spray on the body surface of pigs, floor of the house, air, etc. (mainly acting on the body surface). Spraying on the body surface of pigs can make the bacterial agent directly act on the organic matter on the body surface to decompose the precursor of odor substances; spraying on the floor of the house can clean the residual excrement and other organic matter on the floor to reduce the breeding of putrefactive bacteria; spraying on the air can improve the air quality in the house and reduce the odor concentration. Ensure that the bacterial solution uniformly covers the target area to form a biofilm.

[0056] (5) Spray once every 3-7 days, and adjust according to the odor situation of the breeding environment. If the odor in the breeding environment is heavy, the spraying interval time can be appropriately shortened; if the odor is light, the spraying interval time can be appropriately extended. In summer or when the odor in the pig house is heavy, the spraying frequency can be appropriately increased.

[0057] By installing ammonia gas sensors, humidity sensors and other equipment in the pig house, the environmental parameters in the pig house are monitored in real time, and the operation of the spraying equipment is automatically controlled according to the preset threshold value to realize precise spraying, improve the deodorizing effect, and reduce the labor cost. To ensure that the bacterial solution uniformly covers the target area to form a biofilm. The specific dosage is adjusted according to the age, breed, excrement and urine output of pigs. The older the pigs are, the more excrement and urine they produce, and the more spraying is required. Different breeds of pigs should be adjusted according to the difference coefficient of excrement and urine output to determine the optimal spraying amount. See Table 5 and Table 6. Figure 4

[0058] Table 5 Spraying amount of deodorizing and disinfecting solution for pigs of different ages and different breeds

[0059]

[0060]

[0061] Mechanism of Bacillus subtilis and Aspergillus oryzae in long-term deodorization: ​

[0062] I. Bacillus subtilis: enzymatic degradation of organic macromolecules, blocking the generation of odor precursors

[0063] 1. Protease breaks down proteins

[0064] Reaction formula:

[0065] Mechanism of action: The proteins in sweat and sebum are degraded into small molecule peptides and amino acids, reducing the production of ammonia (NH3), hydrogen sulfide (H2S), and other odor substances by spoilage bacteria.

[0066] 2. Lipase breaks down fats

[0067] Reaction formula:

[0068] Mechanism of action: The degradation of triglycerides in sebum into glycerol and fatty acids avoids the oxidation of fatty acids into volatile fatty acids by odor-producing bacteria, thereby reducing the pungent and sour odor.

[0069] II. Aspergillus oryzae: degradation of starch and phytic acid, acidification of the environment to inhibit spoilage bacteria

[0070] 1. Starch amylase and glucoamylase synergistically degrade carbohydrates

[0071] Reaction formula:

[0072] Mechanism of action: The decomposition of starch attached to feed residues and pig body surface reduces the excessive production of acid by spoilage bacteria utilizing carbohydrates, leading to pH imbalance or the production of CO2, CH4, etc.

[0073] 2. Phytase degrades phytic acid

[0074] Reaction formula:

[0075] Mechanism of action: The decomposition of phytic acid in plant feed releases phosphorus elements for pig absorption, while reducing the indigestible complex formed by the combination of phytic acid and minerals, thereby reducing the odor produced by undigested substances in feces.

[0076] 3. Organic acid metabolic products acidify the environment (mainly lactic acid):

[0077] Mechanism of action: Aspergillus oryzae fermentation produces lactic acid, citric acid, and other organic acids, reducing the pH value of the pig body surface and the environment of the barn (target pH 4.5-5.5), inhibiting the growth of Escherichia coli, Salmonella, and other spoilage bacteria.

[0078] The proteins in the pig body surface or feces are decomposed into amino acids by Bacillus subtilis, and the amino acids are converted into organic acids by Aspergillus oryzae, so that the pH value of the feces is reduced to below 4.5, thereby inhibiting the production of odor by spoilage bacteria (such as Escherichia coli). Through the synergistic effect of "decomposition before fermentation", the source of odor (protein) can be efficiently converted into odorless and beneficial organic acids, and the acidification of the environment further inhibits the growth of odor-producing bacteria.

[0079] Test Example 1: Ammonia detection verification effect.

[0080] Before and after spraying the deodorizing disinfectant, air samples were collected in the pig house, and the ammonia concentration was determined using a portable malodorous gas detector. To ensure the accuracy and reliability of the test results, the air samples were collected strictly in accordance with the operating procedures to avoid contamination. At the same time, environmental temperature, humidity and other parameters were recorded to exclude the influence of external factors on the test results. Changes in temperature and humidity can affect the volatilization of ammonia and the readings of the detector, and by recording these parameters, the test results can be corrected and calibrated.

[0081] During the test, 15 pigs of the same age, same breed and same house were selected as test objects to reduce the influence of individual differences of the pigs on the test results, as shown in Table 6 and Table 7. Figure 5

[0082] Table 6 Comparison of ammonia concentration before and after spraying deodorizing disinfectant in the same environment

[0083]

[0084]

[0085] As shown in Figure 5 The change of ammonia concentration before and after spraying is shown in the figure. According to the spraying amount and accurate proportioning determined by the present application, the deodorizing disinfectant has a significant deodorizing effect after use.

[0086] The average value of the ammonia concentration detected 15 times before spraying was calculated: 5.09 (ppm)

[0087] The average value of the ammonia concentration detected 15 times after spraying was calculated: 3.07 (ppm)

[0088] Comparison shows that the average concentration of ammonia after spraying is reduced by (5.09-3.07) ÷ 5.09 x 100% ≈ 39.7% compared with before spraying, and the deodorizing effect is significant.

[0089] ​Concentration trend analysis: From the test data and the graph, it can be seen that the ammonia concentration fluctuates relatively large before spraying, and the overall is at a high level. After spraying the deodorizing agent, the ammonia concentration decreases rapidly and remains at a relatively stable low level in the subsequent test. This shows that the deodorizing material of the application can quickly and effectively reduce the ammonia concentration in the pig house, and continuously play a deodorizing role.

[0090] It is shown that the use of the method according to the application for deodorizing and disinfecting the pig house can make the ammonia concentration in the pig house reach and be better than the industry standard requirement, and provide a healthier and more comfortable living environment for the pigs. Through the ammonia detection verification, it is fully proved that the deodorizing scheme provided by the application has a significant effect in reducing the ammonia concentration in the pig house and improving the environment of the pig house.

[0091] Example 2

[0092] A method for using a composite probiotic deodorizing and disinfecting solution, the method comprising the following steps:

[0093] (1) making a change curve of pig manure and urine output at different ages;

[0094] (2) making pig manure and urine output coefficients of different breeds;

[0095] (3) according to the results obtained in steps (1) and (2), formulating a deodorizing ingredient ratio scheme, taking the required agent, and preparing the amount according to the weight ratio, the agent including 15 parts of grass bacillus, 30 parts of aspergillus oryzae, 8 parts of activated carbon, 3 parts of citric acid, 3 parts of glucose and 5 parts of glycerol, adding pure water, mixing uniformly, and preparing a deodorizing and disinfecting solution with the required concentration of the agent;

[0096] (4) placing the deodorizing and disinfecting solution in a spraying device, and spraying the surface of the poultry and livestock and their living places to ensure that the bacterial solution uniformly covers the target area and forms a biofilm;

[0097] At the same time, the deodorizing and disinfecting solution is added to the drinking water of the poultry and livestock. By adding the agent to the drinking water, the feces odor is significantly reduced, and the feed utilization rate is improved.

[0098] (5) suitable for Landrace pigs of 29-70 days old, ensuring that the spraying amount is 0.7L / m 2 · day.

[0099] Other places not mentioned are the same as in Example 1.

[0100] Example 3

[0101] A method for using a composite probiotic deodorizing disinfectant solution, a deodorizing ingredient ratio scheme is formulated, the required bacterial agent is taken, and the dosage is prepared according to the weight ratio, the bacterial agent includes 20 parts of grass bacillus and 40 parts of aspergillus oryzae, water, adsorbent, acid-base regulator, nutrient agent and protective agent are added, and the deodorizing disinfectant solution with the required bacterial agent concentration is prepared by mixing uniformly;

[0102] (4) The deodorizing disinfectant solution is placed in a spraying device, and the surface of poultry and livestock and their living places are sprayed to ensure that the bacterial solution uniformly covers the target area and forms a biological membrane;

[0103] (5) Ensure. It is suitable for 71-180 day-old Landrace pigs, and the spraying amount is 1.0 L / m 2 · day.

[0104] The other places not mentioned are the same as in Example 1.

[0105] Example 4

[0106] A method for using a composite probiotic deodorizing disinfectant solution, a deodorizing ingredient ratio scheme is formulated, the required bacterial agent is taken, and the dosage is prepared according to the weight ratio, the bacterial agent includes 20 parts of grass bacillus and 40 parts of aspergillus oryzae, water, adsorbent, acid-base regulator, nutrient agent and protective agent are added, and the deodorizing disinfectant solution with the required bacterial agent concentration is prepared by mixing uniformly;

[0107] (4) The deodorizing disinfectant solution is placed in a spraying device, and the surface of poultry and livestock and their living places are sprayed to ensure that the bacterial solution uniformly covers the target area and forms a biological membrane;

[0108] (5) Ensure. It is suitable for 71-180 day-old Landrace pigs, and the spraying amount is 1.0 L / m 2 · day.

[0109] The other places not mentioned are the same as in Example 1.

[0110] Of course, the above description is not a limitation of the present application, and the present application is not limited to the above examples, and the changes, modifications, additions or replacements made by the person skilled in the art within the essential scope of the present application also belong to the protection scope of the present application.

Claims

1. A method of using a composite probiotic deodorizing disinfectant solution, characterized in that, The use method comprises the following steps: (1) tracking and counting the excrement and urine output of poultry of different ages, and making a curve of the excrement and urine output; (2) determining the excrement and urine output coefficients of poultry of different breeds; (3) according to the results obtained in steps (1) and (2), formulating a deodorizing ingredient ratio scheme, taking the required microbial agent, and preparing the microbial agent according to the weight ratio, adding pure water, adsorbent, acid-base regulator, nutrient agent, and protective agent, and mixing them uniformly to prepare a deodorizing and disinfecting solution with the required concentration of the microbial agent; (4) storing the deodorizing and disinfecting solution in a spraying device, and spraying it on the body surface of the poultry and its living place to ensure that the microbial solution uniformly covers the target area and forms a biofilm; and / or, adding the deodorizing and disinfecting solution to the drinking water of the poultry; (5) spraying once every 3-7 days.

2. A method of using the composite probiotic deodorant disinfectant solution according to claim 1, characterized in that, The poultry includes any one of pigs, sheep, cattle, horses, rabbits, quails, ducks, chickens, and geese.

3. A method of using the composite probiotic deodorant disinfectant solution as claimed in claim 2, wherein, The poultry is pigs.

4. A method of using the composite probiotic deodorant disinfectant solution as claimed in claim 3, wherein, In step (1), the pigs are divided into four stages, i.e., 0-28 days, 29-70 days, 71-180 days, and more than 180 days, and the excrement and urine output of each pig per day is tracked and counted to obtain a curve of the excrement and urine output of pigs of different ages; In step (2), a standard breed of pigs is selected according to the physiological characteristics of different breeds of pigs, and the excrement and urine output coefficients of other breeds of pigs are determined according to the standard breed of pigs.

5. A method of using the composite probiotic deodorant disinfectant solution as claimed in claim 4, wherein, The microbial agent comprises 10-25 parts by weight of Bacillus subtilis and 20-50 parts by weight of Aspergillus oryzae; the viable bacterial count of Bacillus subtilis and Aspergillus oryzae accounts for 40%-60%.

6. A method of using the composite probiotic deodorant disinfectant solution as claimed in claim 4, wherein, The adsorbent is one or a mixture of activated carbon and zeolite; the acid-base regulator is one or a mixture of sodium bicarbonate and citric acid; the nutrient agent is one or a mixture of glucose and amino acid; and the protective agent is one or a mixture of glycerol and algal polysaccharide; in the deodorizing and disinfecting solution, the adsorbent accounts for 5-15 parts by weight, the acid-base regulator accounts for 1-5 parts by weight, the nutrient agent accounts for 3-10 parts by weight, and the protective agent accounts for 2-8 parts by weight.

7. A method of using the composite probiotic deodorant disinfectant solution as claimed in claim 4, wherein, The deodorizing disinfectant is to dilute the bacterial agent with water by 50-100 times, and the active bacteria number in the bacterial agent is ≥10 8 CFU / mL.

8. A method of using the composite probiotic deodorant disinfectant solution as claimed in claim 4, wherein, The spraying amount and interval are determined according to the physiological characteristics of different breeds of pigs, different ages of pigs, and the excrement and urine output; the larger the age and the more the excrement and urine output, the greater the spraying amount.

9. A method of using the composite probiotic deodorant disinfectant solution as claimed in claim 8, wherein, Based on the excrement and urine output curves of pigs of different ages and the breed difference coefficients, the weight ratio of Bacillus subtilis and Aspergillus oryzae in the microbial agent is determined, and an individualized deodorizing ingredient ratio scheme is formulated.

10. A method of using the composite probiotic deodorant disinfectant solution as claimed in claim 1, wherein, The pH value of the deodorizing and disinfecting solution is 5.0-6.5, and after spraying, a biofilm can be formed on the body surface of the poultry, which continuously secretes protease, lipase, amylase, and organic acid.

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