Preparation method of fermented feed microbial inoculum additive

Abstract

The invention relates to the technical field of fermented feed microbial inoculum additives for livestock breeding, in particular to a preparation method of a fermented feed microbial inoculum additive, which comprises the following steps: carrying out steam sterilization on a fermentation tank with a culture medium; judging the temperature transfer degree and reducing the introduction speed of cooling water according to the section length proportion with the same temperature change trend in the first time section and the second time section of the temperature change, and adjusting the forward rotation duration of stirring according to the area proportion of the bacterial liquid plane region of the surface image of the to-be-fermented bacterial liquid conforming to the rotational flow characteristic; determining a nutrient feeding angle and a nutrient feeding speed according to a relative relationship between the inoculation port and the plane area; and sequentially carrying out continuous fermentation, microbial inoculum stabilizing treatment, forming processing and finished product quality detection on the to-be-fermented bacterial liquid to form a finished product of the fermented feed microbial inoculum additive. According to the invention, the number of viable bacteria and metabolites thereof in the fermented feed microbial inoculum additive is increased.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of fermented feed microbial agent additives, and particularly relates to a preparation method of a fermented feed microbial agent additive. BACKGROUND

[0002] As a key supporting product for the green development of modern animal husbandry, the core value of the fermented feed microbial agent additive lies in improving the nutritional structure of feed, enhancing the intestinal microecological balance of animals, and reducing the dependence on antibiotics, so as to realize the dual improvement of breeding efficiency and animal product safety. In the industrialized preparation process of the fermented feed microbial agent additive, the environmental control of the fermentation tank, the optimization of the process parameters and the uniformity of the material mixing are the core links to determine the activity of the microbial agent, the density of the microbial cells and the stability of the product. However, the existing technology only focuses on the monitoring and regulation of the overall temperature inside the fermentation tank, ignores the temperature transfer correlation between the joint of the exhaust pipe and the disc sleeve, and the reduction of the stirring efficiency caused by the increase of the bubbles at the top of the fermentation tank due to the temperature transfer correlation, so that the supplemented nutrients cannot be immediately dispersed and uniformly mixed, and a dead zone is formed at the bottom of the tank. Therefore, it is urgent to develop a fermented feed microbial agent additive preparation method which can realize the quantitative monitoring and accurate regulation of the whole process, so as to improve the product quality stability and production efficiency.

[0003] Chinese Patent Publication No. CN114766597A discloses a method for preparing an antibacterial peptide feed additive by double-mixed fermentation, which comprises the steps of preparing Bacillus licheniformis and Pediococcus acidilactici seed liquid, preparing Bacillus licheniformis and Pediococcus acidilactici fermentation liquid, high-pressure homogenization, separation and ultrafiltration purification, and spray drying. The present application selects specific Bacillus licheniformis (CGMCC No. 24209) and Pediococcus acidilactici for double-mixed fermentation, so that the antibacterial peptide crude product obtained after double-mixed fermentation has high antibacterial titer and good inhibition effect on wheat scab fungus. The physiological characteristics of aerobic bacteria and anaerobic bacteria are complementary during the fermentation of Bacillus licheniformis and Pediococcus acidilactici, so that the activity of the antibacterial peptide crude product obtained after fermentation is stronger. It can be seen that the method for preparing an antibacterial peptide feed additive by double-mixed fermentation has the problems that the heat transfer of the joint of the exhaust pipe and the cooling process of the disc sleeve affect the temperature of the joint of the exhaust pipe, which intensifies the foam layer phenomenon above the bacterial liquid, so that the nutrients pass through the foam layer above the bacterial liquid during the feeding process of the fermentation process, which causes the insufficient supply of nutrients above and the formation of un-mixed area at the bottom of the fermentation tank, and further causes the excessive nutrients of the bacterial species at the bottom of the fermentation tank. SUMMARY

[0004] To this end, the present application provides a preparation method of a fermented feed probiotic additive, which is used to overcome the problem that the heat transfer to the joint of the exhaust pipe and the cooling temperature of the disc sleeve of the fermenter affect the foam layer above the bacterial solution, which causes the nutrients to pass through the foam layer above the bacterial solution and form an unmixed area at the bottom of the fermenter, and further causes the excessive nutrients for the bacteria at the bottom of the fermenter.

[0005] To achieve the above-mentioned purpose, the present application provides a preparation method of a fermented feed probiotic additive, comprising: steam sterilizing the fermenter with the culture medium; obtaining a first time section of temperature change at the joint of the top of the fermenter and the exhaust pipe and a second time section of temperature change at the corresponding position of the disc sleeve of the side wall of the fermenter, respectively, wherein, the corresponding position of the disc sleeve of the side wall of the fermenter is the projection area of the exhaust pipe on the disc sleeve; determining the temperature transfer degree of the disc sleeve to the joint of the exhaust pipe according to the length ratio of the same section of the temperature change trend of the first time section and the second time section; adjusting the inflow speed of the cooling water of the disc sleeve according to the length ratio of the same section of the temperature change trend which does not meet the requirement of the temperature transfer degree; cooling the fermenter at the adjusted inflow speed of the cooling water, and feeding the bacterial solution to be fermented into the inoculation port at the top of the fermenter; stirring and mixing the bacterial solution to be fermented in the fermenter and obtaining the surface image of the bacterial solution to be fermented; adjusting the forward rotation time length of stirring according to the area ratio of the planar area of the bacterial solution to be fermented which meets the cyclone characteristics in the surface image; adjusting the nutrient feeding angle and the nutrient feeding speed according to the relative relationship between the inoculation port and the planar area; continuing to sequentially perform the sustained fermentation, the probiotic stabilization treatment, the molding processing and the finished product quality detection on the bacterial solution to be fermented according to the adjusted nutrient feeding angle and the nutrient feeding speed, so as to form the fermented feed probiotic additive.

[0006] Further, the determination of the temperature transfer degree of the disc sleeve to the joint of the exhaust pipe according to the length ratio of the same section of the temperature change trend of the first time section and the second time section comprises: comparing the length ratio of the same section of the temperature change trend with a preset ratio; If the length proportion of the section with the same temperature change trend is greater than the preset proportion, it is determined that the temperature transfer degree does not meet the requirement, and the cooling water input speed of the disc sleeve is reduced.

[0007] Further, the length proportion of the section with the same temperature change trend is a ratio of the length of the time section with the same temperature change trend to the time length of the first time section, wherein, The temperature change trend same includes: The temperature at the joint of the exhaust pipe in the first time section is in an increasing trend, and the temperature at the corresponding position of the disc sleeve in the second time section is in an increasing trend. The temperature at the joint of the exhaust pipe in the first time section is in a decreasing trend, and the temperature at the corresponding position of the disc sleeve in the second time section is in a decreasing trend.

[0008] Further, the cooling water input speed of the disc sleeve and the length proportion of the section with the same temperature change trend are in a negative correlation.

[0009] Further, the length proportion of the section with the same temperature change trend is a ratio of the length of the time section with the same temperature change trend to the time length of the first time section, wherein, The largest region surrounded by the sampling points meeting the cyclone characteristics on the surface image is determined as the surface stirring region; The area of the surface stirring region is compared with a preset area; If the area of the surface stirring region is greater than the preset area, it is determined that the gas exhaust effect of the exhaust pipe does not meet the requirement, and the forward rotation time of stirring is shortened, wherein, The sampling points meeting the cyclone characteristics are all sampling points in which the rotational linear velocity of the surface sampling points of the fermentation bacteria liquid in unit time is greater than a first preset rotational linear velocity.

[0010] Further, the forward rotation time and the area of the surface stirring region are in a negative correlation.

[0011] Further, the relative relationship between the inoculation port and the planar region is determined to determine the nutrient feeding angle and adjust the nutrient feeding speed, comprising: If the vertically downward projection region of the inoculation port is located outside the surface stirring region, it is determined that the nutrient short circuit flow of the fermentation bacteria liquid is abnormal, the nutrient feeding angle is adjusted from a first angle to a second angle, and the nutrient feeding speed is increased, wherein, The first angle is an included angle between a geometric center of a nozzle of a nutrient feeding device along an extension line in a jet direction and an upper surface of the fermentation bacteria liquid.

[0012] Further, the second angle is an included angle between a line connecting a sampling point with the maximum surface rotational linear velocity of the fermentation bacteria solution and a geometric center of a nozzle of the nutrient feeding device and an upper surface of the fermentation bacteria solution.

[0013] Further, the increasing the feeding speed of the nutrient comprises: comparing the rotational linear velocity with a second preset rotational linear velocity; if the rotational linear velocity is less than the second preset rotational linear velocity, increasing the feeding speed of the nutrient.

[0014] Further, the feeding speed of the nutrient is negatively correlated with the speed value.

[0015] Compared with the prior art, the beneficial effects of the present application are that the present application determines the out-of-sync problem of the joint of the exhaust pipe and the tank body of the fermentation tank caused by material or thickness difference during the cooling process after sterilization of the fermentation tank through the same length ratio of the temperature change trend of the first time section of the temperature change of the joint of the exhaust pipe and the second time section of the temperature change of the corresponding position of the disc sleeve of the side wall of the fermentation tank, determines the risk of excessive thermal stress by comparing the temperature change synchronism of the first time section of the temperature change of the joint of the exhaust pipe and the second time section of the temperature change of the corresponding position of the disc sleeve of the side wall of the fermentation tank, and the microscopic deformation or the decrease of the sealing performance of the joint of the exhaust pipe caused by the thermal stress will directly affect the exhaust smoothness of the exhaust pipe of the fermentation tank. Since the air above the fermentation bacteria solution cannot be discharged in time, it accumulates in the headspace of the fermentation tank. Under high back pressure, these gases that cannot be discharged in time are more easily forced into the upper layer of the fermentation bacteria solution by the high-speed rotating stirring paddle during the stirring process of the fermentation bacteria solution. The gas is dispersed in the upper layer of the fermentation bacteria solution in the form of fine bubbles. The protein and other surface active substances in the liquid quickly wrap the bubbles to form a foam layer. Then, when the vertically downward projection area of the inoculation port is located outside the surface stirring area, high-concentration nutrients will appear abnormal short-circuiting phenomenon when the vertically downward projection area of the inoculation port coincides with the stirring area covered by the foam layer. Therefore, by adjusting the feeding angle of the nutrient from the first angle to the second angle and increasing the feeding speed of the nutrient, the input nutrient enters the inside of the surface stirring area, so that the phenomenon of the nutrient directly passing through the foam layer formed by the bubbles wrapped by the protein and other surface active substances in the liquid is reduced by the radial shear force of the surface stirring area.

[0016] Further, the present application reduces the inflow speed of the cooling water of the disc sleeve when the length ratio of the section with the same temperature change trend is larger, reduces the temperature change rate of the disc sleeve, weakens the temperature transfer strength of the disc sleeve to the joint of the exhaust pipe, further reduces the problem of the joint of the exhaust pipe and the thermal expansion and contraction of the fermenter, avoids the influence of thermal stress on the material of the joint of the exhaust pipe, thereby protecting the sealing and structural integrity of the joint of the exhaust pipe, and preventing the problem of micro-deformation or sealing decline caused by thermal stress.

[0017] Further, when the area of the surface stirring area is greater than the preset area, it indicates that the air at the top of the fermenter cannot be discharged in time, accumulates in the top space of the fermenter, and causes the internal back pressure of the fermenter to rise. Under normal exhaust conditions, the vortex generated by the stirring paddle forms a relatively concentrated surface stirring area, but when the back pressure rises, the back pressure will resist the force of the stirring paddle pulling the liquid downward, causing the vortex of the surface stirring area to be flattened, the central area of the vortex, i.e. the horizontal expansion of the surface stirring area, the area of the surface stirring area increases, by shortening the forward rotation time of the stirring, breaking the single direction of the fermentation broth, reducing the disturbance intensity of the stirring paddle to the surface of the fermentation broth, thereby causing the vortex to wrap the air above the fermentation broth that cannot be discharged in time into the upper layer of the fermentation broth, thereby further hindering the foam layer problem caused by the path of the gas rising vertically to the exhaust pipe, promoting the release of the wrapped gas from the fermentation broth and gathering and rising, and ultimately improving the gas discharge effect of the exhaust pipe.

[0018] Further, the present application adjusts the nutrient feeding angle and the nutrient feeding speed based on the disturbance range of the stirring paddle. Since one of the roots of the nutrient directly penetrating the foam layer formed by the bubbles rapidly wrapped by the protein and other surface active substances in the liquid is the misalignment of the nutrient feeding position and the sampling point with the maximum rotational speed on the surface stirring area of the stirring paddle, if the nutrient falls on the point with small rotational speed on the surface stirring area, the nutrient cannot be dispersed in time by the disturbance of the stirring paddle, but can only sink to the lower layer by relying on the density advantage of the nutrient, thereby making the amount of nutrient in the upper layer of the fermentation broth less than that in the lower layer of the fermentation broth. By adjusting the nutrient feeding angle to the second angle between the connecting line of the sampling point with the maximum surface rotational speed on the fermentation broth and the geometric center of the nozzle of the nutrient feeding device and the upper surface of the fermentation broth, the nutrient enters the position on the surface stirring area with the maximum speed after feeding. When the high-concentration nutrient solution falls on the position on the surface stirring area with the maximum rotational speed, it will be rapidly cut and dispersed into small droplets by the strong stirring vortex. After the droplet size is reduced, the density advantage is weakened, and the nutrient solution will diffuse in the disturbance range of the stirring paddle, avoiding the problem of the nutrient solution directly penetrating the foam layer and sinking. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 The overall flow chart of the preparation method of the fermentation feedstuff bacterial agent additive of the embodiment of the present application; Figure 2 The structure schematic diagram of the fermentation tank of the preparation method of the fermentation feedstuff bacterial agent additive of the embodiment of the present application; Figure 3 The flow chart of the adjustment of the forward rotation time length of the stirring process of the bacterial liquid to be fermented of the preparation method of the fermentation feedstuff bacterial agent additive of the embodiment of the present application; Figure 4 The flow chart of the increase of the feeding speed of the nutrients of the preparation method of the fermentation feedstuff bacterial agent additive of the embodiment of the present application; Figure 5 The horizontal sectional view of the disc sleeve and the exhaust pipe of the preparation method of the fermentation feedstuff bacterial agent additive of the embodiment of the present application; The label explanation: 1-fermentation tank, 2-exhaust pipe, 3-disc sleeve, 4-steam pipe, 5-cooling water inlet pipe, 6-cooling water valve, 7-cooling water outlet pipe, 8-stirring paddle, 9-inoculation port, 10-industrial camera, 11-motor, 12-sampling pipe, 13-discharge pipe, 14-discharge valve, 15-observation hole, 16-first temperature sensor, 17-second temperature sensor, 18-projection area. DETAILED DESCRIPTION

[0020] In order to make the purpose and the advantage of the present application more clear and obvious, the present application is further described below by combining with the embodiments; it should be understood that the specific embodiments described here are only used for explaining the present application, and are not used for limiting the present application.

[0021] The preferred embodiments of the present application are described below by referring to the drawings. The skilled in the art should understand that these embodiments are only used for explaining the technical principles of the present application, and are not used for limiting the protection scope of the present application.

[0022] It should be noted that, in the description of the present application, the terms of the directions or the position relationship indicated by the terms of "up", "down", "left", "right", "inner", "outer" and the like are based on the directions or the position relationship shown in the drawings, which are only for the convenience of the description, and are not used for indicating or implying that the device or the element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as the limitation of the present application.

[0023] Moreover, it needs to be explained that in the description of the present application, unless otherwise explicitly specified and limited, the terms "mount", "connect", "connection" should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integrally connected; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through intermediate medium, or the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0024] Please refer to Figure 1 and Figure 5 respectively, the preparation method of the fermentation feed probiotic additive, comprising: Step S1: steam sterilization is performed on the fermenter 1 with culture medium; Step S2: a first time section of temperature change at the joint of the top of the fermenter 1 and the exhaust pipe 2 and a second time section of temperature change at the corresponding position of the disc sleeve 3 of the side wall of the fermenter 1 are obtained respectively, wherein, The corresponding position of the disc sleeve 3 of the side wall of the fermenter 1 is the projection area 18 of the exhaust pipe 2 on the disc sleeve 3; Step S3: according to the length ratio of the same temperature change trend section of the first time section and the second time section, the temperature transfer degree of the disc sleeve 3 to the joint of the exhaust pipe 2 is determined; Step S4: according to the length ratio of the same temperature change trend section that does not meet the requirement of the temperature transfer degree, the inflow speed of the cooling water of the disc sleeve 3 is adjusted; Step S5: the fermenter 1 is cooled at the adjusted inflow speed of the cooling water, and the fermenting bacteria liquid is poured into the inoculation port 9 at the top of the fermenter 1; Step S6: the fermenting bacteria liquid in the fermenter 1 is stirred and mixed, and a surface image of the fermenting bacteria liquid is obtained; Step S7: according to the area ratio of the planar area of the fermenting bacteria liquid meeting the cyclone characteristics in the surface image, the forward rotation time length of stirring is adjusted; Step S8: according to the relative relationship between the inoculation port 9 and the planar area, the nutrient feeding angle and the nutrient feeding speed are adjusted; Step S9: the fermenting bacteria liquid is continuously fermented, probiotic stabilized, formed and finished product quality detected in sequence according to the adjusted nutrient feeding angle and nutrient feeding speed, so as to form the fermentation feed probiotic additive.

[0025] Specifically, the tank volume of the fermenter 1 is 15m 3 , the working volume is 11m 3 , the material of the fermenter 1 is SUS 316L stainless steel, which is used for medium sterilization and fermentation process.

[0026] Specifically, the fermented bacteria liquid which has been processed according to the abnormal feeding mode continues to be subjected to the sustained fermentation in the fermenter 1, and the fermentation time is 12-48 hours.

[0027] Specifically, the bacteria agent stabilization treatment process adopts a double-screw mixing machine, a vacuum concentration tank for removing part of the water in a low-temperature vacuum environment, a low-temperature refrigerator for pre-cooling the mixed bacteria agent, and a moisture meter for real-time monitoring of the moisture content of the bacteria agent.

[0028] Specifically, the forming process adopts a swing granulator for forming the granular bacteria agent, a fluidized bed dryer, and a granular cooling machine.

[0029] Specifically, the finished product quality detection process adopts a biological safety cabinet, a colony counter and other detection equipment.

[0030] Those skilled in the art can understand that the operating principle and process of the equipment used in the processes of the fermented bacteria liquid subjected to the sustained fermentation, the bacteria agent stabilization treatment, the forming process and the finished product quality detection are conventional technical means known to those skilled in the art, and therefore the operating principle and process of the equipment used in the processes of the fermented bacteria liquid subjected to the sustained fermentation, the bacteria agent stabilization treatment, the forming process and the finished product quality detection will not be described here.

[0031] Please refer to Figure 2 , which is a structural schematic diagram of the fermenter of the preparation method of the fermented feed bacteria agent additive according to the embodiment of the present application, and the fermenter 1 further comprises: A stirring paddle 8 is arranged inside the fermenter 1, the stirring paddle 8 is a three-layer six-straight-blade disc turbine, the diameter of the stirring paddle 8 is 0.5m, the rotating speed is 50-300rpm, the stirring paddle 8 is powered by an electric motor 11, the electric motor 11 is an explosion-proof variable frequency motor, the rated power is 22kW, and the frequency converter inside the electric motor 11 is used to control the rotating speed and realize the automatic control of the forward and reverse rotation; An exhaust pipe 2 is arranged at the top of the fermenter 1, which is used to discharge steam, and a first temperature sensor 16 is arranged at the joint of the exhaust pipe 2; A disc sleeve 3 is arranged in a half-ring around the outer wall of the tank body of the fermenter 1, which is used to pass in cooling water or steam, the covering area of the disc sleeve 3 on the fermenter 1 is 8m 3 , and a second temperature sensor 17 is arranged at the corresponding position of the disc sleeve 3; Steam pipe 4, which is connected with the disc sleeve 3, is used to pass high-pressure steam for sterilization, and the material of the steam pipe 4 is 304 stainless steel; Cooling water inlet pipe 5, which is connected with the disc sleeve 3, is used to pass cooling water, and the material of the cooling water inlet pipe 5 is 304 stainless steel; Cooling water valve 6, which is arranged on the cooling water inlet pipe 5, is an electric regulating valve, which can adjust the opening degree to control the cooling water flow according to the control signal; Cooling water outlet pipe 7, which is connected with the disc sleeve 3, is used to discharge the cooling water in the disc sleeve 3, and the material of the cooling water outlet pipe 7 is consistent with that of the cooling water inlet pipe 5; Industrial camera 10, which is arranged at the observation hole 15 at the top of the fermentation tank 1, has a shooting direction of facing the liquid surface of the bacterial liquid, and is used to continuously collect the image of the bacterial liquid surface; Inoculation port 9, which is arranged at the top of the fermentation tank 1, is used to put the bacterial liquid to be fermented and the nutrients for the bacterial liquid to be fermented. After the bacterial liquid to be fermented is put in, the nutrient feeding device is connected during the stirring process of the bacterial liquid to be fermented. The nutrient feeding device includes an adjustable nozzle and a liquid tank (not shown in the figure) controlled by a metering pump to control the feeding speed of the nutrients; Sampling pipe 12, which is connected with the side wall of the fermentation tank 1, is used to monitor the density of the bacterial body during the fermentation process. The diameter of the sampling pipe 12 is 4-8mm, and the material is consistent with that of the steam pipe 4; Discharge pipe 13 and discharge valve 14, which are arranged at the bottom of the fermentation tank 1, are used to discharge the finished product of the fermented feed probiotic additive after the fermentation process is completed.

[0032] Specifically, the culture medium in the fermentation tank 1 is a special culture medium for bacillus, and the volume of the culture medium is 11m 3 .

[0033] In implementation, the present application determines the problem of thermal expansion and contraction of the joint of the exhaust pipe 2 and the tank body of the fermenter 1 caused by material or thickness difference during the cooling process after sterilization of the fermenter 1 by the length ratio of the same segment of the temperature change trend of the first time segment of the temperature change of the joint of the exhaust pipe 2 and the second time segment of the temperature change of the corresponding position of the disc sleeve 3 of the side wall of the fermenter 1, determines the risk of excessive thermal stress by comparing the synchronization of the temperature change of the first time segment of the temperature change of the joint of the exhaust pipe 2 and the second time segment of the temperature change of the corresponding position of the disc sleeve 3 of the side wall of the fermenter 1, and the microscopic deformation or sealing decline of the joint of the exhaust pipe 2 caused by thermal stress will directly affect the exhaust smoothness of the exhaust pipe 2 of the fermenter 1. Because the air above the fermentation liquid cannot be discharged in time, it accumulates in the headspace of the fermenter 1, and under high back pressure, these gases that cannot be discharged in time are more easily forced into the upper layer of the fermentation liquid by the high-speed rotating stirring paddle 8 during the stirring process of the fermentation liquid. The gas is dispersed in the upper layer of the fermentation liquid in the form of fine bubbles, and the protein and other surface active substances in the liquid quickly wrap the bubbles to form a foam layer. When the vertically downward projection area of the inoculation port 9 is located outside the surface stirring area, and when the vertically downward projection area of the inoculation port 9 coincides with the stirring area covered by the foam layer, high-concentration nutrients will appear abnormal conditions such as layer-penetrating short-circuiting. Therefore, by adjusting the nutrient feeding angle from the first angle to the second angle and increasing the nutrient feeding speed, the nutrients enter the inside of the surface stirring area, so that the phenomenon of nutrients directly penetrating through the foam layer formed by the rapid wrapping of bubbles by protein and other surface active substances in the liquid is reduced by the radial shear force of the surface stirring area.

[0034] Specifically, the length ratio of the same segment of the temperature change trend of the first time segment and the second time segment determines the temperature transfer degree of the disc sleeve 3 to the temperature of the joint of the exhaust pipe 2, comprising: comparing the length ratio of the same segment of the temperature change trend with the preset ratio; if the length ratio of the same segment of the temperature change trend is greater than the preset ratio, it is determined that the temperature transfer degree does not meet the requirements, and the inlet speed of the cooling water of the disc sleeve 3 is reduced.

[0035] Specifically, the time length of the first time segment and the second time segment is the same.

[0036] Specifically, the length ratio of the same segment of the temperature change trend is the ratio of the length of the time segment of the same temperature change trend to the time length of the first time segment, wherein the same temperature change trend includes: The temperature at the joint of the exhaust pipe 2 in the first time segment is in an increasing trend, and the temperature at the corresponding position of the disc sleeve 3 in the second time segment is in an increasing trend; The temperature at the joint of the exhaust pipe 2 in the first time segment is in a decreasing trend, and the temperature at the corresponding position of the disc sleeve 3 in the second time segment is in a decreasing trend.

[0037] Specifically, if the temperature at the joint of the exhaust pipe 2 in the first time segment is continuously increasing / decreasing in the first time segment, it is determined that the temperature at the joint of the exhaust pipe 2 is in an increasing / decreasing trend; If the temperature at the corresponding position of the disc sleeve 3 in the second time segment is continuously increasing / decreasing in the second time segment, it is determined that the temperature at the corresponding position of the disc sleeve 3 is in an increasing / decreasing trend.

[0038] Optionally, the preset proportion range is [50%, 70%].

[0039] Preferably, the preferred embodiment of the preset proportion is 60%.

[0040] Specifically, the cooling water inlet speed of the disc sleeve 3 is negatively correlated with the proportion of the length of the segment with the same temperature change trend.

[0041] In implementation, when the proportion of the length of the segment with the same temperature change trend exceeds the value of the preset proportion by 10% or less, the cooling water inlet speed of the disc sleeve 3 is adjusted to 90% of the current inlet speed, and when the proportion of the length of the segment with the same temperature change trend exceeds the value of the preset proportion by more than 10% every 5%, the inlet speed is reduced by 0.05%. In a specific embodiment, the proportion of the length of the segment with the same temperature change trend is 75%, the current inlet speed is 20 L / min, and the reduced inlet speed is 20 L / min x 90% x (1-0.05%) = 17.991 L / min. When the calculated cooling water inlet speed of the disc sleeve 3 has more than one decimal place, it is rounded to the first decimal place, i.e. 18.0 L / min.

[0042] In implementation, the present application reduces the cooling water inlet speed of the disc sleeve 3 when the proportion of the length of the segment with the same temperature change trend is larger, reduces the temperature change rate of the disc sleeve 3, weakens the temperature transfer intensity of the disc sleeve 3 to the joint of the exhaust pipe 2, further reduces the problem of the joint of the exhaust pipe 2 and the thermal expansion and contraction of the fermenter 1 being out of sync, avoids the influence of thermal stress on the material of the joint of the exhaust pipe 2, thereby protecting the sealing performance and structural integrity of the joint of the exhaust pipe 2, and preventing the problem of microscopic deformation or reduced sealing performance caused by thermal stress.

[0043] Please refer to Figure 3As shown, it is a flow chart of adjusting the forward rotation time length of the stirring process of the to-be-fermented bacterial liquid in the preparation method of the fermentation feed bacterial agent additive according to the embodiment of the application, and the forward rotation time length of the stirring is adjusted according to the area ratio of the planar area of the to-be-fermented bacterial liquid meeting the cyclone characteristics in the surface image, and the method comprises the following steps of: determining the maximum area surrounded by the sampling points meeting the cyclone characteristics on the surface image as a surface stirring area; comparing the area of the surface stirring area with a preset area; if the area of the surface stirring area is greater than the preset area, it is determined that the gas discharge effect of the exhaust pipe 2 does not meet the requirements, and the forward rotation time length of the stirring is shortened, wherein the sampling points meeting the cyclone characteristics are all sampling points whose rotational linear velocity of the surface sampling points of the to-be-fermented bacterial liquid in a unit time is greater than a first preset rotational linear velocity.

[0044] Optionally, the range of the preset area is [0.85m 2 , 1.38m 2 ]; and the range of the first preset rotational linear velocity is [0.1m / s, 0.3m / s].

[0045] Preferably, the preferred embodiment of the preset area is 1.04m 2 ; and the preferred embodiment of the first preset rotational linear velocity is 0.2m / s.

[0046] Specifically, the forward rotation time length is in a negative correlation with the area of the surface stirring area.

[0047] In the implementation, when the area of the surface stirring area exceeds the value of the preset area within 0.2m 2 , the forward rotation time length is adjusted to 90% of the current forward rotation time length, when the area of the surface stirring area exceeds the value of the preset area greater than 0.2m 2 , the forward rotation time length is reduced by 0.05% for each 0.1m 2 , in a specific embodiment, the area of the surface stirring area is 1.5m 2 , the current forward rotation time length is 80 seconds, and the reduced forward rotation time length is 80 seconds*90%*(1-0.13%)=71.9 seconds, when the calculated forward rotation time length has a decimal, rounding is performed, that is, 72 seconds.

[0048] In the implementation, when the area of the surface stirring area is greater than the preset area, it indicates that the air at the top of the fermenter 1 cannot be discharged in time, accumulates in the top space of the fermenter 1, and causes the internal back pressure of the fermenter 1 to rise. Under normal exhaust conditions, the vortex generated by the stirring paddle 8 forms a relatively concentrated surface stirring area. When the back pressure rises, the back pressure resists the force of the stirring paddle 8 pulling the liquid downward, causing the vortex of the surface stirring area to be flattened, the central area of the vortex, that is, the surface stirring area, to expand horizontally, and the area of the surface stirring area to increase. By shortening the forward rotation time of the stirring, the single-direction rotational inertia of the fermentation bacteria liquid is broken, the disturbance intensity of the fermentation bacteria liquid surface caused by the forward rotation of the stirring paddle 8 is reduced, the rotational flow of the fermentation bacteria liquid cannot timely discharge the air above the fermentation bacteria liquid, thereby further hindering the foam layer problem caused by the path of the gas rising vertically to the exhaust pipe 2, promoting the gas trapped from the fermentation bacteria liquid to release and gather upward, and finally improving the gas discharge effect of the exhaust pipe 2.

[0049] Specifically, the determination of the nutrient feeding angle and the adjustment of the nutrient feeding speed according to the relative relationship between the inoculation port 9 and the planar area include: If the vertically downward projection area of the inoculation port 9 is located outside the surface stirring area, it is determined that the nutrient short-circuit flow of the fermentation bacteria liquid is abnormal, the nutrient feeding angle is adjusted from the first angle to the second angle, and the nutrient feeding speed is increased, wherein, The first angle is the included angle between the extension line of the geometric center of the nozzle of the nutrient feeding device in the jet direction and the upper surface of the fermentation bacteria liquid.

[0050] Specifically, the second angle is the included angle between the line connecting the sampling point with the maximum surface rotational speed of the fermentation bacteria liquid and the geometric center of the nozzle of the nutrient feeding device and the upper surface of the fermentation bacteria liquid.

[0051] Specifically, the nutrient feeding angle is adjusted through the rotatable hinge structure on the adjustable angle nozzle of the nutrient feeding device, and the nutrient feeding angle is rotated to the target angle by adjusting the direction of the fixing bolt.

[0052] Please refer to Figure 4 The preparation method of the fermentation feed bacteria additive of the embodiment of the present application is shown in the flowchart of increasing the nutrient feeding speed, and the increasing of the nutrient feeding speed includes: Comparing the rotational speed with the second preset rotational speed; If the rotational speed is less than the second preset rotational speed, the nutrient feeding speed is increased.

[0053] Optionally, the second preset rotating linear velocity is in a range of [0.25 m / s, 0.4 m / s].

[0054] Preferably, the preferred embodiment of the second preset rotating linear velocity is 0.3 m / s.

[0055] As can be understood by those skilled in the art, the optional range and the preferred embodiment of the second preset rotating linear velocity are based on the optional range and the preferred embodiment of the viscosity characteristics of the fermentation broth to be fermented, and those skilled in the art can adaptively adjust the second preset rotating linear velocity according to specific application scenarios.

[0056] Specifically, the feeding speed of the nutrient is negatively correlated with the rotating linear velocity.

[0057] In implementation, when the rotating linear velocity is less than the second preset rotating linear velocity by a value within 0.1 m / s, the feeding speed of the nutrient is adjusted to 1.1 times the current feeding speed of the nutrient, and when the rotating linear velocity is less than the second preset rotating linear velocity by a value greater than 0.1 m / s, the feeding speed of the nutrient is increased by 0.05% for each 0.05 m / s. In a specific embodiment, the rotating linear velocity is 0.15 m / s, the current feeding speed of the nutrient is 3.0 m / s, and the increased feeding speed of the nutrient is 3.0 m / s x 1.1 x (1+0.05%) = 3.30165 m / s. When the calculated feeding speed of the nutrient has more than two decimal places, it is rounded to two decimal places, i.e. 3.30 m / s.

[0058] In implementation, the present application adjusts the nutrient feeding angle and the feeding speed of the nutrient based on the disturbance range of the stirring paddle 8. Since one of the reasons why the nutrient directly penetrates the foam layer formed by the rapid wrapping of gas bubbles by the protein and other surface active substances in the liquid is that the feeding position of the nutrient is misaligned with the sampling point with the maximum rotating speed on the surface stirring area of the stirring paddle 8, if the nutrient falls on a point with a small rotating speed on the surface stirring area, the nutrient cannot be dispersed in time by the disturbance of the stirring paddle 8, but can only sink to the lower layer by virtue of the density advantage of the nutrient, thereby making the amount of nutrient in the upper layer of the fermentation broth less than the amount of nutrient in the lower layer of the fermentation broth. By adjusting the nutrient feeding angle to the second angle between the line connecting the sampling point with the maximum rotating linear velocity on the surface of the fermentation broth and the geometric center of the nozzle of the nutrient feeding device and the upper surface of the fermentation broth, the nutrient enters the position on the surface stirring area with the maximum entering speed after feeding. When the high-concentration nutrient solution falls on the position on the surface stirring area with the maximum rotating linear velocity, it will be rapidly cut and dispersed into fine droplets by strong stirring flow. After the droplet size is reduced, the density advantage is weakened, and the nutrient solution will diffuse in the disturbance range of the stirring paddle 8, thereby avoiding the problem of direct penetration of the nutrient solution through the foam layer.

[0059] Working process: the fermentation tank 1 containing the culture medium is subjected to steam sterilization to build a sterile fermentation environment; after sterilization, the temperature changes of the joint of the exhaust pipe 2 at the top of the fermentation tank 1 and the corresponding position of the side wall disc sleeve 3 are monitored synchronously, and the time segments of the temperature changes of the two are recorded respectively, the length proportion of the same temperature change trend of the two time segments is analyzed, the temperature transfer degree of the disc sleeve 3 to the joint of the exhaust pipe 2 is judged, if the transfer degree does not meet the requirements, the cooling water inlet speed is reduced, the fermentation tank 1 is cooled according to the adjusted cooling water inlet speed, and the fermentation bacteria liquid is poured into the inoculation port 9 at the top of the fermentation tank 1; the fermentation bacteria liquid in the tank is stirred and mixed, and the surface image of the fermentation bacteria liquid is collected, based on the surface image, the sampling points meeting the cyclone characteristics are selected, that is, all the sampling points with a rotational linear velocity greater than a first preset rotational linear velocity in a unit time, and the maximum area surrounded by these sampling points is determined as the surface stirring area; the area of the surface stirring area is compared with the preset area, if the area of the surface stirring area is greater than the preset area, it is determined that the gas discharge effect of the exhaust pipe 2 does not meet the requirements, the forward rotation time of stirring needs to be shortened, then according to the relative relationship between the vertical downward projection area of the inoculation port 9 and the surface stirring area, the nutrient feeding angle and the nutrient feeding speed are adjusted: if the vertical downward projection area of the inoculation port 9 is located outside the surface stirring area, it is determined that the nutrient short circuit flow of the fermentation bacteria liquid is abnormal, the nutrient feeding angle needs to be adjusted from the first angle to the second angle, and the nutrient feeding speed needs to be increased, when the nutrient feeding speed is increased, the rotational linear velocity of the sampling point with the maximum rotational linear velocity is compared with the second preset rotational linear velocity, if the rotational linear velocity is less than the second preset rotational linear velocity, the feeding speed is increased, finally, the fermentation bacteria liquid is subjected to continuous fermentation, inoculant stabilization treatment, molding processing and finished product quality detection in turn according to the adjusted nutrient feeding angle and nutrient feeding speed, and finally the fermentation feed inoculant additive is formed.

[0060] So far, the technical scheme of the present application has been described in combination with the preferred embodiments shown in the drawings, but those skilled in the art can easily understand that the protection scope of the present application is obviously not limited to these specific embodiments. Those skilled in the art can make equivalent changes or replacements to the related technical features without departing from the principles of the present application, and the technical schemes after these changes or replacements will all fall within the protection scope of the present application.

Claims

1. A method for preparing a fermented feed microbial agent additive, characterized in that, include: The fermenter containing the culture medium was steam sterilized; The temperature change at the joint between the top of the fermenter and the exhaust pipe is acquired in a first time period, and the temperature change at the corresponding position on the sidewall of the fermenter is acquired in a second time period. The corresponding position of the disc sleeve on the side wall of the fermenter is the projection area of ​​the exhaust pipe on the disc sleeve; The degree of temperature transfer from the disc sleeve to the joint of the exhaust pipe is determined based on the proportion of the length of the section with the same temperature change trend in the first time segment and the second time segment. The cooling water flow rate of the disc sleeve is adjusted according to the proportion of the length of the section with the same temperature change trend where the temperature transfer degree does not meet the requirements. The fermenter is cooled according to the adjusted cooling water flow rate, and the fermentation liquid is added to the inoculation port at the top of the fermenter. The fermentation liquid inside the fermenter is stirred and mixed, and a surface image of the fermentation liquid is obtained. Adjust the forward rotation time of the stirring according to the area ratio of the planar region of the bacterial liquid to be fermented that conforms to the swirling characteristics in the surface image; Adjust the nutrient delivery angle and nutrient delivery speed according to the relative relationship between the inoculation port and the planar area; Following the adjusted nutrient delivery angle and speed, the fermentation broth was continuously fermented, stabilized, shaped, and the finished product was tested to form a fermented feed microbial additive.

2. The method for preparing the fermented feed microbial agent additive according to claim 1, characterized in that, Based on the proportion of lengths in the first and second time periods where the temperature change trends are the same, the degree of temperature transfer from the disc sleeve to the joint of the exhaust pipe is determined, including: Compare the percentage of lengths of segments with the same temperature change trend with a preset percentage; If the proportion of the length of the sections with the same temperature change trend is greater than the preset proportion, it is determined that the degree of temperature transmission does not meet the requirements, and the cooling water flow rate of the disc sleeve is reduced.

3. The method for preparing the fermented feed microbial agent additive according to claim 2, characterized in that, The proportion of the length of the time interval with the same temperature change trend is the ratio of the length of the time interval with the same temperature change trend to the length of the first time interval, wherein, The same temperature change trend includes: The temperature at the joint of the exhaust pipe in the first time period shows an increasing trend, and the temperature at the corresponding position of the disc sleeve in the second time period also shows an increasing trend. In the first time period, the temperature at the joint of the exhaust pipe shows a decreasing trend, and in the second time period, the temperature at the corresponding position of the disc sleeve also shows a decreasing trend.

4. The method for preparing the fermented feed microbial agent additive according to claim 3, characterized in that, The rate at which cooling water is introduced into the sleeve is negatively correlated with the proportion of the section length that follows the same temperature change trend.

5. The method for preparing the fermented feed microbial agent additive according to claim 4, characterized in that, The step of adjusting the forward rotation time of the stirring according to the area ratio of the planar region of the bacterial broth to be fermented that conforms to the swirling characteristics in the surface image includes: The largest region enclosed by the sampling points that conform to the swirling characteristics on the surface image is defined as the surface stirring region; The area of ​​the surface stirring region is compared with the preset area; If the area of ​​the surface stirring zone is larger than the preset area, it is determined that the gas discharge effect of the exhaust pipe does not meet the requirements, and the forward rotation time of the stirring is shortened. The sampling points that meet the swirling flow characteristics are all sampling points whose rotational linear velocity on the surface of the bacterial liquid to be fermented is greater than the first preset rotational linear velocity per unit time.

6. The method for preparing the fermented feed microbial agent additive according to claim 5, characterized in that, The forward rotation time is negatively correlated with the area of ​​the surface stirring region.

7. The method for preparing the fermented feed microbial agent additive according to claim 6, characterized in that, The step of determining the nutrient delivery angle and adjusting the nutrient delivery speed based on the relative relationship between the inoculation port and the planar area includes: If the vertically downward projection area of ​​the inoculation port is located outside the surface stirring area, it is determined that the nutrient short-circuit flow of the bacterial culture to be fermented is abnormal. The nutrient delivery angle is adjusted from the first angle to the second angle, and the nutrient delivery speed is increased. The first angle is the angle formed by the extension of the geometric center of the nozzle of the nutrient delivery device along the spray direction and the upper surface of the bacterial liquid to be fermented.

8. The method for preparing the fermented feed microbial agent additive according to claim 7, characterized in that, The second angle is the angle formed by the line connecting the sampling point with the maximum rotational linear velocity on the surface of the bacterial liquid to be fermented and the geometric center of the nozzle of the nutrient delivery device, and the upper surface of the bacterial liquid to be fermented.

9. The method for preparing the fermented feed microbial agent additive according to claim 8, characterized in that, Increasing the rate of nutrient delivery includes: The rotational linear velocity is compared with the second preset rotational linear velocity; If the rotational linear velocity is less than the second preset rotational linear velocity, then the nutrient delivery speed is increased.

10. The method for preparing the fermented feed microbial agent additive according to claim 9, characterized in that, The rate at which nutrients are delivered is negatively correlated with the rotational linear velocity.

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