Anti-pollution composition containing guaiazulene as well as preparation method and application of anti-pollution composition

Abstract

The invention relates to the technical field of skin care products, in particular to an anti-pollution composition containing guaiazulene as well as a preparation method and application of the anti-pollution composition. The composition is formed by compounding guaiazulene lipidosome, a kelp extract, a phoenix dactylifera seed extract and a kadsura longepedunculata extract, and triple protection is realized through cascade action of the four components. The composition not only can realize the common effect of adsorption, anti-inflammation, repair and soothing, but also has a considerable soothing effect on skin sensitivity, pruritus and red and swollen caused by company decoration. The preparation method of the composition provided by the invention is simple and easy to operate, is beneficial to industrial actual large-scale production and application, meets market requirements, and has relatively high market application value.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care products, in particular to an anti-pollution composition containing guaiazulene and a preparation method and application thereof. BACKGROUND

[0002] With the development of human civilization, people pay more and more attention to a healthy lifestyle, and the frequency and enthusiasm of participating in outdoor activities also increase. However, according to the statistics of the World Health Organization, more than 80% of the air quality in the world's major cities does not meet the standards, and the problem of air pollution is becoming increasingly serious. Nowadays, more and more skin problems are closely related to urban environmental pollution. Industrial emissions, motor vehicle exhaust and various environmental factors produce smoke dust particles that can penetrate the skin, damage skin cells and cause a variety of skin problems.

[0003] In the field of cosmetics, in order to solve the problem of skin being damaged by external pollution and inflammation, etc., various means have been adopted in the prior art. The mainstream solution of current anti-pollution skin care products focuses on single-dimensional physical isolation or chemical antagonistic strategies. Silicon-containing polymers, such as dimethicone, block PM2.5 and other particulate matter through film formation, but long-term use can damage the skin microecological balance, leading to an increase in hair follicle blockage rate, and is completely ineffective against water-soluble pollutants such as formaldehyde and benzene series. Antioxidant ingredients such as vitamin C derivatives, Ve, and ergothioneine can neutralize free radicals, but cannot block the aromatic hydrocarbon receptor (AhR) signaling pathway activated by pollutants. This pathway is the core mechanism for inducing the release of inflammatory factors IL-6 / IL-8, and the inhibition rate of existing products is less than 40%. Traditional soothing ingredients can only temporarily suppress redness, but cannot repair the damaged stratum corneum lipid structure caused by pollutants, and the barrier damage is difficult to repair. More seriously, these technologies generally ignore the risk of microbial co-contamination, such as the formation of composite pollutants by heavy metals and harmful bacteria or fungi in a decorated environment, and the antibacterial rate of products on the market is generally low, and they lack the ability to actively repair the skin barrier.

[0004] These problems reflect the urgent need for a soothing and anti-pollution skin care composition in the cosmetics industry to achieve soothing and anti-pollution effects, thereby meeting the urgent demand for high-efficiency, safe and fast-acting products in the market. SUMMARY

[0005] The present application aims to overcome the shortcomings of the prior art and provide an anti-pollution composition containing guaiazulene and its application. The raw material components in the composition synergize with each other, and the prepared composition not only achieves the common effect of "adsorption-anti-inflammation-repair-soothing", but also has a considerable soothing effect on skin sensitivity, itching and swelling induced by office decoration.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows:

[0007] In a first aspect, the present application provides an anti-pollution composition containing guaiazulene, comprising the following components: guaiazulene liposomes, giant kelp extract, date seed extract, and schisandra extract, wherein the weight ratio of the guaiazulene liposomes, the giant kelp extract, the date seed extract, and the schisandra extract is (0.01-1):(0.01-3):(0.1-2):(0.01-3).

[0008] Guaiazulene is a deep blue crystalline hydrocarbon compound extracted from the chrysanthemum plant (such as German chamomile), which belongs to the chamomile ring derivative. Its core mechanism is to inhibit the release of inflammatory factors IL-1α, TNF-α, and MPO, block the inflammatory cascade reaction, and up-regulate the expression of repair factors TGF-β1 and VEGF to accelerate skin barrier reconstruction and vascular regeneration. By reducing capillary permeability through antihistamine action, reducing the pruritus signal transduction mediated by neuropeptide P substance, and directly inhibiting the activation of capsaicin receptor TRPV1, the burning and stinging sensation is relieved. In addition, guaiazulene can inhibit the release of 5-hydroxytryptamine, weaken the edema reaction induced by allergens, and has rapid soothing and tissue regeneration effect on burns, scalds, chemical stimulation, and radiation damage.

[0009] The giant kelp extract is derived from the giant kelp in the Antarctic, which is rich in fucoidan and alginate. It forms a physical pollution barrier film by chelating heavy metal ions (such as lead and cadmium) and adsorbing PM2.5 particles, reducing environmental toxin penetration. At the molecular level, it can inhibit the expression of metalloproteinase (MMP-1 / 3), block the degradation of collagen induced by ultraviolet light and pollutants, and reduce wrinkle formation. Fucoidan polysaccharide can enhance the immune response of keratinocytes by activating TLR4 receptor, inhibit the colonization of pathogenic microorganisms such as Pseudomonas aeruginosa, and reduce infectious redness.

[0010] The date seed extract takes polyphenol and gallic acid as the main active ingredients, which can enhance the water-locking ability of the deep epidermis by activating aquaporin AQP3 and increase the water content of the stratum corneum. It promotes the expression of filaggrin and hyaluronic acid synthase (HAS2), repairs the damaged lipid structure between keratinocytes, and reduces trans-epidermal water loss (TEWL). Molecular mechanism research shows that the extract can down-regulate the activity of NF-κB pathway, inhibit the release of pro-inflammatory factors such as IL-6 and IL-8, and relieve neurogenic redness caused by chemical stimulation. It synergistically regulates HGF / VEGF growth factors, accelerates collagen deposition and epithelial regeneration at the wound site.

[0011] Schisandra chinensis extract takes biphenylphene lignans such as schizandrin A and schizandrin B as the core active ingredients. It can scavenge DPPH free radicals and inhibit lipid peroxidation, block ROS oxidative damage to fibroblasts, and reduce photoaging collagen fragmentation. Key mechanisms include down-regulating TNF-α / IL-1β induced by β-amyloid, inhibiting excessive activation of NF-κB signaling pathway, and reducing neurogenic inflammatory response. Schisandra chinensis extract can antagonize the expression of histamine H1 receptor and relieve allergen-triggered capillary dilation. In addition, schisantherin B can enhance the activity of superoxide dismutase (SOD) and improve the skin's own antioxidant defense system.

[0012] Preferably, the weight ratio of the guaiazulene liposome, the giant kelp extract, the Phoenix dactylifera seed extract, and the Schisandra chinensis extract is (0.05-0.5):(0.05-2):(0.5-1.2):(0.05-1.5).

[0013] More preferably, the weight ratio of the guaiazulene liposome, the giant kelp extract, the Phoenix dactylifera seed extract, and the Schisandra chinensis extract is (0.1-0.3):(0.1-1):(0.6-0.8):(0.1-0.5).

[0014] In a second aspect, the present application provides use of the anti-pollution composition of the first aspect in the preparation of a skin care product.

[0015] Preferably, the skin care product is any one of a toner, an emulsion, a cream, a mask, an essence, or a spray, and the anti-pollution composition is added in an amount of 0.5%-5% of the total weight of the skin care product.

[0016] In a third aspect, the present application provides an essence, which comprises the following raw materials in the following weight percentages: 0.5%-5% of the anti-pollution composition of the first aspect, 0.05%-0.3% of a thickening agent, 0.5%-10% of a humectant, 0.5%-1% of a preservative, and 0.01%-0.3% of a pH adjuster, and the balance is deionized water.

[0017] Preferably, the thickening agent comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / acryloyldimethyltaurate sodium copolymer, acryloyldimethyltaurate ammonium / VP copolymer, and arthrobacter gum.

[0018] Preferably, the humectant comprises at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerol, pullulan, and ceramide.

[0019] Preferably, the pH adjuster comprises at least one of arginine, tromethamine, and disodium EDTA.

[0020] Preferably, the preservative comprises at least one of 1,3-propanediol, 1,2-hexanediol and p-hydroxyacetophenone.

[0021] In a fourth aspect, the present application provides a preparation method of the serum in the third aspect, comprising the following steps:

[0022] S1, mixing the humectant, thickening agent and part of deionized water, homogenizing and uniformly mixing to obtain an aqueous phase solution;

[0023] S2, after the temperature of the aqueous phase solution in S1 is reduced to 55-65 DEG C, adding a preservative, stirring uniformly, and when the temperature of the system is reduced to 38-42 DEG C, adding each component in the anti-pollution composition of the first aspect and the remaining deionized water, stirring uniformly, finally adding a pH adjuster to adjust the pH to obtain the serum.

[0024] Preferably, the homogenization temperature in the step S1 is 75-85 DEG C.

[0025] Preferably, the pH in the step S2 is adjusted to 5.5-6.5.

[0026] Compared with the prior art, the present application has the following beneficial effects:

[0027] The present application provides an anti-pollution composition containing gualile, which is composed of gualile liposome, kelp extract, date seed extract and schisandra extract. The triple protection is realized through the cascade effect of the four components. The fucoidan in the kelp extract forms a negative-charged network film on the skin surface, captures PM2.5 and chelates heavy metal ions (such as lead and cadmium) through electrostatic adsorption, thereby reducing the penetration of pollutants from the source. The gualile liposome can target inhibit the release of TNF-alpha / IL-1 alpha downstream of the AhR pathway, and up-regulate the synthesis of filaggrin, thereby repairing the damaged lipid structure of the stratum corneum and shortening the recovery time of the barrier. The schisandra extract removes active oxygen induced by pollutants and blocks the NF-kappa B inflammatory cascade, thereby blocking oxidation and neurogenic redness. The date seed extract activates aquaporin AQP3, increases the water content of the stratum corneum, regulates the skin microecology, inhibits the colonization of pathogenic microorganisms, activates alpha-2A adrenergic receptors involved in vasoconstriction, and relieves redness and itching. The composition not only has the combined effect of "adsorption-anti-inflammatory-repair-soothing", but also has a significant soothing effect on skin sensitivity, itching and redness induced by company decoration.

[0028] The preparation method of the composition provided by the present application is simple and easy to operate, is conducive to industrialized large-scale production and application, meets market demand, and has strong market application value. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1The application example 1 group of essence liquid before and after use is used for testing the use comparison chart of the volunteers in example 4. DETAILED DESCRIPTION

[0030] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples.

[0031] The raw materials used in the following examples and comparative examples are as follows:

[0032] The guaiazulene liposome is from Nan Kang Biotechnology Co., Ltd., and the trade name is "stable guaiazulene";

[0033] The extract of giant kelp is from Guangzhou Haishi Fine Chemical Co., Ltd., and the trade name is SEACTIVE TM Algae amoxicillin;

[0034] The date seed extract is from Lacus merey company, and the trade name is IBR-CalmDeAge;

[0035] The schisandra chinensis extract is from Draco company.

[0036] Other materials, reagents, etc. used in the examples can be obtained from commercial channels if there is no special description.

[0037] The component composition (parts by weight) of the skin care composition in examples 1-7 and comparative examples 1-8 in the present application is shown as follows, each component is weighed according to the formula amount, mixed and homogenized to obtain each composition.

[0038] Example 1

[0039] An anti-pollution composition, comprising guaiazulene liposome, giant kelp extract, date seed extract and schisandra chinensis extract in a weight ratio of 0.2:0.5:0.7:0.3, the total weight parts of the guaiazulene liposome, giant kelp extract, date seed extract and schisandra chinensis extract is 100 parts.

[0040] Example 2

[0041] An anti-pollution composition, comprising guaiazulene liposome, giant kelp extract, date seed extract and schisandra chinensis extract in a weight ratio of 0.1:0.1:0.6:0.1, the total weight parts of the guaiazulene liposome, giant kelp extract, date seed extract and schisandra chinensis extract is 100 parts.

[0042] Example 3

[0043] An anti-pollution composition comprising liposomes of guaiazulene, a giant kelp extract, a Phoenix dactylifera seed extract, and a Schisandra chinensis extract in a weight ratio of 0.3:1:0.8:0.5, the total weight fraction of the liposomes of guaiazulene, the giant kelp extract, the Phoenix dactylifera seed extract, and the Schisandra chinensis extract being 100 parts.

[0044] Example 4

[0045] An anti-pollution composition comprising liposomes of guaiazulene, a giant kelp extract, a Phoenix dactylifera seed extract, and a Schisandra chinensis extract in a weight ratio of 0.05:0.05:0.5:0.05, the total weight fraction of the liposomes of guaiazulene, the giant kelp extract, the Phoenix dactylifera seed extract, and the Schisandra chinensis extract being 100 parts.

[0046] Example 5

[0047] An anti-pollution composition comprising liposomes of guaiazulene, a giant kelp extract, a Phoenix dactylifera seed extract, and a Schisandra chinensis extract in a weight ratio of 0.5:2:1.2:1.5, the total weight fraction of the liposomes of guaiazulene, the giant kelp extract, the Phoenix dactylifera seed extract, and the Schisandra chinensis extract being 100 parts.

[0048] Example 6

[0049] An anti-pollution composition comprising liposomes of guaiazulene, a giant kelp extract, a Phoenix dactylifera seed extract, and a Schisandra chinensis extract in a weight ratio of 0.01:0.01:2:3, the total weight fraction of the liposomes of guaiazulene, the giant kelp extract, the Phoenix dactylifera seed extract, and the Schisandra chinensis extract being 100 parts.

[0050] Example 7

[0051] An anti-pollution composition comprising liposomes of guaiazulene, a giant kelp extract, a Phoenix dactylifera seed extract, and a Schisandra chinensis extract in a weight ratio of 1:3:0.1:0.01, the total weight fraction of the liposomes of guaiazulene, the giant kelp extract, the Phoenix dactylifera seed extract, and the Schisandra chinensis extract being 100 parts.

[0052] Comparative Example 1

[0053] Comparative Example 1 differs from Example 1 in that no liposomes of guaiazulene are added, and the missing amount is made up with a giant kelp extract, a Phoenix dactylifera seed extract, and a Schisandra chinensis extract in a weight ratio of 0.5:0.7:0.3.

[0054] Comparative Example 2

[0055] Comparative Example 2 differs from Example 1 in that no giant kelp extract is added, and the missing amount is made up with liposomes of guaiazulene, a Phoenix dactylifera seed extract, and a Schisandra chinensis extract in a weight ratio of 0.2:0.7:0.3.

[0056] Comparative Example 3

[0057] Comparative Example 3 differs from Example 1 in that no Phoenix dactylifera seed extract is added, and the missing amount is made up with a weight ratio of 0.2:0.5:0.3 of guaiazulene liposome, Laminaria japonica extract, and Schisandra chinensis extract.

[0058] Comparative Example 4

[0059] Comparative Example 4 differs from Example 1 in that no Schisandra chinensis extract is added, and the missing amount is made up with a weight ratio of 0.2:0.5:0.7 of guaiazulene liposome, Laminaria japonica extract, and Phoenix dactylifera seed extract.

[0060] Comparative Example 5

[0061] Comparative Example 5 differs from Example 1 in that no guaiazulene liposome is added, and an equal amount of bisabolol is substituted.

[0062] Comparative Example 6

[0063] Comparative Example 6 differs from Example 1 in that no Laminaria japonica extract is added, and an equal amount of Sargassum fusiforme extract is substituted.

[0064] Comparative Example 7

[0065] Comparative Example 7 differs from Example 1 in that no Phoenix dactylifera seed extract is added, and an equal amount of Vitis vinifera seed extract is substituted.

[0066] Comparative Example 8

[0067] Comparative Example 8 differs from Example 1 in that no Schisandra chinensis extract is added, and an equal amount of Gynostemma pentaphyllum extract is substituted.

[0068] Test Example 1: Inhibitory effect of the composition on inflammation induced by air pollution

[0069] Filaggrin (FLG) is an important molecule in the stratum corneum of human skin that connects keratin fibers. With the help of FLG monomers, keratin fibers are regularly aggregated to form a solid physical barrier in the outermost layer of the epidermis, playing a role in strengthening the skin. Inflammatory factor IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. It can promote the proliferation and differentiation of various cells, accelerate the synthesis of acute phase proteins in hepatocytes, and inhibit the growth of melanoma and breast cancer cells. Polycyclic aromatic hydrocarbons in the air are common environmental pollutants, and benzo(a)pyrene (BaP) is one of the most studied polycyclic aromatic hydrocarbons and a major environmental factor that causes skin damage and inflammation. Therefore, the more FLG barrier proteins and the less inflammatory factor IL-6 after the composition is treated, the stronger the anti-air pollution ability of the composition. The barrier protection ability of the skin care composition is represented by the improvement of FLG, and the anti-inflammatory ability is represented by the improvement of IL-6.

[0070] This effect example explores the inhibitory effect of the anti-pollution composition prepared in Examples 1-7 and Comparative Examples 1-8 on inflammation and barrier damage caused by air pollution.

[0071] The cell line used is human keratinocyte HaCaT (BeNa Biotech), and the test conditions are: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%; the cells are cultured and treated according to the grouping, and then tested. The specific tests are the detection of inflammatory factor IL-6 content and the detection of barrier protein FLG gene, and the test methods are as follows:

[0072] (1) The cell suspension was inoculated in a 96-well cell culture plate at a density of 2000 cells / well, and 100 μL of DMEM medium was added to each well, and cultured for 24 h;

[0073] (2) Discard the supernatant, add 100 μL of DMEM medium containing 1 mM BaP (Sigma-Aldrich, item number B1760-500MG) to the control group, and add DMEM medium containing 1 mM BaP and 0.01wt% of the sample to be tested (skin care compositions prepared in Examples 1-7 and Comparative Examples 1-8) to the sample group;

[0074] (3) After 24 h of incubation, the culture medium and cells were collected respectively;

[0075] (4) Barrier protein FLG gene detection: RNA of cells was extracted using an RNA extraction kit (TRIzol, Thermo), and reverse transcription of RNA into cDNA was performed using a reverse transcription kit (RevertAid RT, Thermo) to synthesize cDNA. Real-time fluorescent quantitative PCR reaction (TaqMan, Thermo) was performed using the synthesized cDNA as a template. Real-time fluorescent quantitative PCR was used to detect the FLG gene expression of HaCaT cells in each group. GAPDH was used as an internal reference gene. The FLG primer sequence used was 5'-TGAAGCCTATGACACCACTGA-3', 5'-TCCCCTACGCTTTCTTGTCCT-3'. The GAPDH primer sequence used was 5'-ACTTTGTCAAGCTCATTTCC-3', 5'-TGCAGCGAACTTTATTGATG-3'.

[0076] (5) Inflammatory factor IL-6 detection: Human IL-6 ELISA kit (EHC007.96, Shenzhen Xingboshen Biotechnology Co., Ltd.) was used to detect the content of inflammatory factor IL-6 released by cells in the culture solution.

[0077] (6) Calculate the FLG improvement rate and IL-6 improvement rate, the formula is as follows:

[0078] FLG improvement rate = (FLG expression 样品组 - FLG 对照组 / FLG expression 对照组 ) x 100%;

[0079] IL-6 improvement rate = (1-IL-6 content 样品组 / IL-6 content 对照组 ) x 100%;

[0080] The results are shown in Table 1.

[0081] Test Example 2: Inhibition of oxidative stress caused by air pollution by the composition

[0082] Particulate matter is one of the main pollutants in the air, and air pollution can accumulate on the surface of the skin, triggering a series of oxidative stress reactions, leading to absorption through hair follicles or diffusion to deeper dermal layers through inhalation. Once these pollutants enter the skin, they can trigger an increase in reactive oxygen species, induce oxidative stress, and possibly lead to the collapse of the defense system and the development of tissue damage. The less ROS produced after treatment with a skin care composition, the stronger the inhibition of oxidative stress caused by air pollution by the skin care composition. The inhibition of oxidative stress by the anti-pollution composition is represented by the improvement of ROS.

[0083] The effect example explores the inhibition of oxidative stress caused by air pollution of the anti-pollution composition prepared in Examples 1-7 and Comparative Examples 1-8.

[0084] The cell line used is human keratinocyte HaCaT (BeNa Biotech), and the test conditions are: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%; the cells are cultured and treated according to the grouping, and then tested, and the specific test is the content of reactive oxygen species ROS, and the test method is as follows:

[0085] (1) The cell suspension was inoculated in a 96-well cell culture plate at a density of 2000 cells per well, and 100 μL of DMEM medium was added to each well, and cultured for 24 h;

[0086] (2) The supernatant was discarded, and 100 μL of DMEM medium containing 100 μg / mL fine particulate matter (Sigma-Aldrich, item number NIST2975) was added to the control group (2105341, Gibco), and 100 μg / mL particulate matter and 0.01 wt% of the sample to be tested (skin care compositions prepared in Examples 1-7 and Comparative Examples 1-8, respectively) were added to the sample group;

[0087] (3) After 24 h of incubation, the culture medium and cells were collected;

[0088] (4) The reactive oxygen species (ROS) test kit (chemical fluorescence method) (Enzyme-linked Biotech, ml092661) was used to determine the reactive oxygen species ROS of the model group and the sample group, and the operation was carried out according to the instructions of the reactive oxygen test kit (chemical fluorescence method, DCFH-DA probe): 100 μL of 10 μM DCFH-DA working solution was added to each well of the control group and the sample group, and incubated at 37℃ for 30 min in the dark; the probe solution was aspirated and washed with PBS for 3 times to remove excess probe; immediately measure the fluorescence intensity (A value) with a fluorescence microplate reader (excitation wavelength 502 nm, emission wavelength 530 nm), and read 3 times per well to take the average value;

[0089] (6) The ROS improvement rate is calculated according to the following formula:

[0090] ROS improvement rate (%) = (1-ROS 样品组 / ROS 对照组 ) × 100%;

[0091] The results are shown in Table 1.

[0092] Table 1 Data of the inhibition of inflammation and oxidative stress caused by air pollution of the composition

[0093]

[0094]

[0095] From the data of FLG improvement rate, IL-6 improvement rate and ROS improvement rate of Examples 1-7 and Comparative Examples 1-8 in Table 1, it can be seen that the FLG improvement rate, IL-6 improvement rate and ROS improvement rate of the composition prepared by compounding the four ingredients of azulene liposome, giant kelp extract, date seed extract and schisandra extract are all significantly improved, indicating that the four ingredients of azulene liposome, giant kelp extract, date seed extract and schisandra extract have synergistic effect; the absence or replacement of any one of the four ingredients will reduce the anti-pollution effect of the composition; compared with Example 1, Comparative Example 2 and Comparative Example 3 lack giant kelp extract and date fruit extract respectively, so their effects on FLG improvement are poor; compared with Example 1, Comparative Example 1 and Comparative Example 4 lack azulene liposome and schisandra extract respectively, so their anti-inflammatory and antioxidant abilities are the worst; in summary, the compounding of the four ingredients of azulene liposome, giant kelp extract, date seed extract and schisandra extract can achieve triple protection, so that the soothing stress and anti-pollution effects of the composition are enhanced.

[0096] Application Examples 1-7 and Comparative Application Examples 1-9

[0097] The compositions of Examples 1-7 and Comparative Examples 1-8 are added to the serum at a concentration of 3wt%, to obtain the serum of Application Examples 1-7 and Comparative Application Examples 1-8, and the formula is shown in Table 2.

[0098] The preparation method of the serum of Application Examples 1-7 and Comparative Application Examples 1-8, comprising the following steps:

[0099] The preparation method of the serum, comprising the following steps:

[0100] S1, mix the humectant, thickening agent and 1 / 2 amount of deionized water, and homogenize at 80℃ to obtain an aqueous solution;

[0101] S2, after the temperature of the aqueous solution in S1 drops to 60℃, add the preservative and stir evenly, and when the system temperature drops to 40℃, add each component in the skin care composition and the remaining deionized water, stir evenly, and finally add the pH adjuster to adjust the pH to 6 to obtain the serum.

[0102] Table 2 Formulas of the serum of Application Examples 1-7 and Comparative Application Examples 1-8

[0103]

[0104] Comparative Application Example 9

[0105] The difference between Comparative Application Example 9 and Application Example 1 is that the essence liquid of Comparative Application Example 9 does not add the composition, and an equal amount of deionized water is used instead of the composition, and the preparation method is the same as that of Application Example 1.

[0106] Test Example 3: Human long-acting soothing and repairing effect

[0107] Experimental method: According to the general principles of human safety inspection methods for cosmetics in the "Cosmetic Safety Technology Standard", 48 Asian adult volunteers aged 18-60 years who felt that their skin was sensitive were selected and randomly divided into 16 groups, with 3 people in each group. On the day of the volunteer visit, the test area was washed with water without applying any product, and the volunteers sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. The Tewameter TM Hex probe, the CM 825 probe and the MX 18 probe were used to test the TEWL, moisture content and erythema index (a* value) of the malar bone;

[0108] Long-term test: The subjects used the essence liquid twice a day, morning and evening, each time 2 pumps, about 1g, and were re-visited after 14 days, and the TEWL, moisture content and erythema index (a* value) of the malar bone were tested;

[0109] The improvement rate of each value before and after use = |mean value before use-mean value after use| / mean value before use x 100%;

[0110] The calculated data are shown in Table 3 below;

[0111] Table 3 Test data of essence liquids of Application Examples 1-7 and Comparative Application Examples 1-9

[0112]

[0113]

[0114] From the data of Application Examples 1-7 and Comparative Application Examples 1-8 in Table 3, it can be seen that the TEWL improvement rate, moisture improvement rate and erythema improvement rate of Application Examples 1-8 prepared by using the composition of guaiazulene liposomes, giant kelp extract, date seed extract and schisandra chinensis extract are significantly improved, indicating that guaiazulene liposomes, giant kelp extract, date seed extract and schisandra chinensis extract have synergistic effect, and the soothing and irritating ability of the composition is enhanced.

[0115] Test Example 4: Human soothing effect on redness and itching caused by decoration pollution allergy

[0116] Experimental method: 3 volunteers who had skin allergy caused by company decoration were recruited, and 1g of the essence liquid prepared by Application Example 1 was applied to the allergic position, and the soothing effect of redness and itching was observed within 5 minutes.

[0117] Results as shown in Table 1, the redness of 3 volunteers were significantly eased, and all expressed a significant reduction in itching, indicating that the serum prepared by the application example 1 has excellent soothing effect. Figure 1

[0118] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application, but not to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the present application.​

Claims

1. An anti-pollution composition containing guaiazulene, characterized in that, The anti-pollution composition comprises the following components: guaiazulene liposome, giant kelp extract, date seed extract and schisandra chinensis extract, and the weight ratio of the guaiazulene liposome, the giant kelp extract, the date seed extract and the schisandra chinensis extract is (0.01-1) : (0.01-3) : (0.1-2) : (0.01-3).

2. The anti-pollution composition according to claim 1, wherein The weight ratio of the guaiazulene liposome, the giant kelp extract, the date seed extract and the schisandra chinensis extract is (0.05-0.5) : (0.05-2) : (0.5-1.2) : (0.05-1.5).

3. The anti-pollution composition of claim 1, wherein The weight ratio of the guaiazulene liposome, the giant kelp extract, the date seed extract and the schisandra chinensis extract is (0.1-0.3) : (0.1-1) : (0.6-0.8) : (0.1-0.5).

4. Use of the anti-pollution composition according to any one of claims 1-3 in the preparation of a skin care product.

5. Use of the anti-pollution composition according to claim 4 for the preparation of a skin care product, characterized in that, The skin care product is any one of a toner, an emulsion, a cream, a mask, an essence or a spray, and the addition amount of the skin care composition is 0.5%-5% of the total weight of the skin care product.

6. An essence characterized by, The skin care product comprises the following raw materials in the following weight percentages: 0.5%-5% of the anti-pollution composition according to any one of claims 1-3, 0.05%-0.3% of a thickening agent, 0.5%-10% of a humectant, 0.5%-1% of a preservative and 0.01%-0.3% of a pH regulator, and the balance is deionized water.

7. The serum of claim 6, wherein the serum is a serum for skin care. The raw materials are selected from at least one of (a)-(d): (a) the thickening agent comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer and arthrobacter gum; (b) the humectant comprises at least one of allantoin, betaine, beta-glucan, trehalose, glycerol, propylene glycol, sodium hyaluronate, 1,3-butanediol, glycerol, pullulan and ceramide; (c) the pH regulator comprises at least one of arginine, tromethamine and disodium EDTA; (d) the preservative comprises at least one of 1,3-propanediol, 1,2-hexanediol and p-hydroxyacetophenone.

8. The method of preparing the serum according to claim 6 or 7, characterized in that, The method comprises the following steps: S1, mixing the humectant, the thickening agent and part of the deionized water, homogenizing and uniformly mixing to obtain an aqueous phase solution; S2, after the temperature of the aqueous phase solution in S1 is reduced to 55-65℃, adding the preservative and stirring uniformly, and when the temperature of the system is reduced to 38-42℃, adding the components in the skin care composition and the remaining deionized water, stirring uniformly, and finally adding the pH regulator to adjust the pH to obtain the essence.

9. The production method according to claim 8, wherein The homogenization temperature in step S1 is 75-85℃.

10. The production method according to claim 8, wherein The pH in step S2 is adjusted to 5.5-6.5.

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