Synergistic anti-aging composition based on white truffle fungus extract, hydrolyzed keratin and soluble collagen and preparation method thereof

Abstract

The invention provides a synergistic anti-aging composition based on a white truffle fungus extract, hydrolyzed keratin and soluble collagen and a preparation method of the synergistic anti-aging composition. The essence comprises the following components in percentage by mass: 0.5-5% of a white truffle fungus extract, 1-8% of hydrolyzed keratin and 2-10% of soluble collagen, and is supplemented with cross-linked modified hyaluronic acid, glycerol, ceramide, tocopheryl acetate, phenoxyethanol and a solvent. The preparation method comprises the following steps: preparing the cross-linked modified hyaluronic acid, performing low-temperature enzymolysis extraction on the white truffle bacteria, mixing and dissolving, and preparing the composition. The anti-aging effect of the composition is effectively improved and the stability of the composition is improved through the ternary synergistic effect (the white truffle bacteria can resist oxidation, the hydrolyzed keratin can repair a barrier and the collagen can promote regeneration) and process optimization.

Description

Technical Field

[0001] This invention belongs to the field of cosmetics and skin anti-aging technology, specifically relating to a synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin and soluble collagen and its preparation method. Background Technology

[0002] White truffle ( Tuber magnatum Pico White truffle is a rare underground symbiotic fungus, mainly distributed in the roots of oak and hazel trees in specific regions of Europe. Its unique aromatic compounds and rich bioactive components (such as polysaccharides and polyphenols) make it highly sought after in the high-end cosmetics industry. Studies have shown that white truffle extract has significant antioxidant, anti-inflammatory, and epidermal cell repair-promoting functions, showing particular potential in delaying skin aging and reducing free radical damage, and is considered "natural gold" among anti-aging ingredients.

[0003] However, the application of white truffles in cosmetics still faces multiple challenges. First, traditional extraction processes (such as water extraction, alcohol extraction, or high-temperature drying) can easily lead to the degradation of heat-sensitive active ingredients (such as polyphenols), reducing their antioxidant efficacy. Second, existing formulations often employ single-ingredient compounding strategies (such as combining only with hyaluronic acid or vitamin C), lacking multi-target synergistic effects and making it difficult to simultaneously achieve antioxidant, barrier repair, and collagen regeneration. In addition, unpurified crude extracts may contain impurities (such as protein fragments or inorganic salts), which may cause skin irritation or reduce product stability.

[0004] To address the aforementioned issues, existing technologies are mainly improved through three approaches: First, supercritical CO2 extraction technology is used to extract at low temperatures to preserve heat-sensitive components; second, nanoliposome or microcapsule encapsulation technology is used to enhance the transdermal absorption and stability of active ingredients; and third, white truffle extract is combined with other anti-aging ingredients (such as plant extracts or synthetic peptides) through compound formulation technology in an attempt to improve the overall effect.

[0005] Taking nanoliposome encapsulation technology as an example, although this method can effectively improve the transdermal absorption rate of active ingredients, its industrial application still has significant drawbacks. On the one hand, the preparation process of nanoliposomes is complex, requiring precise control of particle size distribution (usually ≤200 nm), resulting in high production costs and poor batch stability. On the other hand, liposome encapsulation may alter the release kinetics of white truffle active ingredients, such as premature release on the skin surface or insufficient deep penetration, thereby weakening its antioxidant and repair effects. More importantly, existing technologies lack systematic research on the synergistic effects of white truffle with other anti-aging ingredients, making it difficult to simultaneously achieve antioxidant, barrier repair, and collagen regeneration.

[0006] Therefore, there is an urgent need for a solution that can efficiently preserve the active ingredients of white truffle fungi, achieve integrated anti-aging of antioxidant, barrier repair and collagen regeneration through multi-component synergistic effect, and has simple process and controllable cost. SUMMARY

[0007] The purpose of the present application is to provide a synergistic anti-aging composition based on white truffle fungus extract, hydrolyzed keratin and soluble collagen and a preparation method thereof. The composition has the advantages of deep penetration, multi-target anti-aging and significant synergistic effect. Through the synergistic effect of white truffle fungus extract, hydrolyzed keratin and soluble collagen, combined with optimized extraction and preparation process, the integrated anti-aging technology of antioxidant, collagen regeneration and barrier function repair is realized.

[0008] To achieve the above purpose, the present application provides the following technical solutions: A synergistic anti-aging composition based on white truffle fungus extract, hydrolyzed keratin and soluble collagen, comprising the following components by mass percentage: white truffle fungus extract 0.5%-5%, hydrolyzed keratin 1%-8%, soluble collagen 2%-10%, excipient 2%-15%, and ethanol-water mixed solvent to make up the rest to 100%.

[0009] Further, the excipient comprises the following components by mass percentage: cross-linked modified hyaluronic acid 0.5%-4%, glycerol 5%-10%, ceramide NP 0.05%-1.5%, tocopheryl acetate 0.2%-2%, and phenoxyethanol 0.1%-0.5%.

[0010] Preferably, the anti-aging composition comprises the following components by mass percentage: white truffle fungus extract 4%, hydrolyzed keratin 7%, soluble collagen 10%, cross-linked modified hyaluronic acid 2%, glycerol 10%, ceramide NP 1%, tocopheryl acetate 1%, phenoxyethanol 0.2%, and ethanol-water mixed solvent to make up the rest to 100%.

[0011] Further, the volume percentage of ethanol in the ethanol-water mixed solvent is 30%-50%.

[0012] Further, the cross-linked modified hyaluronic acid is prepared by carbodiimide cross-linking, and the addition amount of the carbodiimide is 8%-12% of the mass of hyaluronic acid, and the cross-linking reaction time is 1.5-2.5 hours.

[0013] Further, the molecular weight of the hydrolyzed keratin is 800-1500 Da, and the soluble collagen is type I or type III collagen.

[0014] The application also provides a preparation method of the synergistic anti-aging composition based on the trichloroacetic acid extract of white truffle, hydrolyzed keratin and soluble collagen, comprising the following steps: a. Preparation of cross-linked modified hyaluronic acid: first, dissolve the hyaluronic acid in a phosphate buffer with a pH of 5.5-6.0, add carbodiimide, and stir at 30-35°C for 2 hours; then, after dialysis purification, freeze-drying is performed to obtain cross-linked modified hyaluronic acid; b. Enzymatic extraction of white truffle extract: slice fresh white truffle and dry at 45°C to a moisture content of ≤5%; after crushing, add a citric acid buffer with a pH of 5.0, add cellulase at a material ratio of 1:50, and enzymatically hydrolyze for 2.5 hours; after enzyme inactivation, filtration and concentration to 1 / 3 of the original volume, the white truffle extract is obtained; c. Mixing and dissolving: mix the hydrolyzed keratin and soluble collagen, dissolve in deionized water, and stir for 40 minutes to obtain a mixed solution; d. Preparation of the composition: mix the cross-linked modified hyaluronic acid, white truffle extract, mixed solution, excipients and ethanol-water mixed solvent; first, perform ultrasonic treatment and then high-pressure homogenization; adjust the pH to 6.0, filter sterilization, and obtain the anti-aging composition.

[0015] Further, the dialysis bag with a molecular weight cut-off of 10 kDa is used for dialysis purification in step a, and the purification time is 24 hours.

[0016] Further, the viscosity of the mixed solution in step c is 50-100 mPa·s.

[0017] Further, the ultrasonic treatment in step d has a frequency of 40 kHz, a power of 250 W, and a treatment time of 25 minutes; the high-pressure homogenization treatment has a pressure of 40 MPa, a cycle number of 6 times, and a temperature of 55°C.

[0018] Compared with the prior art, the application has the following advantages and beneficial effects: 1. The anti-aging composition of the application adopts a ternary synergistic design of hydrolyzed keratin (500-2000 Da), soluble collagen (type I / III) and white truffle extract, uses the charge characteristics of hydrolyzed keratin and the hydrophobic region of collagen to form a self-assembled penetration network, accurately regulates the transdermal release and targeted distribution of active ingredients; the white truffle extract removes free radicals, and the hydrolyzed keratin repairs the epidermal barrier; the soluble collagen promotes dermal regeneration, and the three components synergistically achieve the integrated anti-aging effect of antioxidant, barrier repair and collagen regeneration, effectively improving the anti-aging effect; 2. The anti-aging composition of the present invention uses cross-linked modified hyaluronic acid as one of the excipients. Carbodiimide cross-linked modified hyaluronic acid is used to construct a three-dimensional network structure to replace the traditional liposome encapsulation technology, thereby avoiding the problem of active ingredient inactivation caused by liposome membrane rupture. At the same time, it significantly enhances the moisturizing durability (moisturizing time extended to 12 hours) and environmental stability (room temperature storage ≥24 months). 3. The anti-aging composition of the present invention uses cellulase hydrolysis combined with low-temperature drying technology to decompose the cell wall of white truffle fungus and extract active ingredients (such as polyphenols and polysaccharides) under mild conditions during the preparation process. This avoids the damage to heat-sensitive ingredients caused by traditional high-temperature or supercritical CO2 processes, while significantly reducing equipment costs and energy consumption, and effectively improving the retention of active ingredients. 4. In the preparation process of the anti-aging composition of the present invention, the active ingredients are uniformly dispersed at the nanoscale through the synergistic effect of ultrasound and high-pressure homogenization, without the need for complex nano-encapsulation processes, thereby improving the stability of the anti-aging composition. Detailed Implementation

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Example 1 This embodiment provides a synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen. By mass percentage, it comprises the following components: 4% white truffle extract, 7% hydrolyzed keratin (molecular weight 1200 Da), 10% soluble collagen (type III), 2% cross-linked modified hyaluronic acid, 10% glycerin, 1% ceramide NP, 1% tocopheryl acetate, 0.2% phenoxyethanol, and an ethanol-water mixed solvent (ethanol volume percentage 40%) to make up the balance to 100%.

[0021] The method for preparing this anti-aging composition includes the following steps: a. Preparation of cross-linked modified hyaluronic acid: Hyaluronic acid (molecular weight of 200 kDa) was first dissolved in phosphate buffer at pH 5.5-6.0, and 10% carbodiimide by weight of hyaluronic acid was added. The mixture was stirred at 30°C for 2 hours. Then, the mixture was purified by dialysis using a dialysis bag with a molecular weight cutoff of 10 kDa for 24 hours and then freeze-dried to obtain cross-linked modified hyaluronic acid. b. Enzymatic extraction of white truffle extract: Fresh white truffles were sliced ​​and dried at 45℃ until the moisture content was ≤5%; pulverized to 150 mesh, added to a citrate buffer solution with pH 5.0, and cellulase (enzyme activity 500 U / g) was added at a material ratio of 1:50. Enzymatic hydrolysis was carried out at 52℃ for 2.5 hours; the enzyme was inactivated at 80℃, filtered, and concentrated to 1 / 3 of the volume to obtain white truffle extract; c. Mixing and dissolving: Mix hydrolyzed keratin with soluble collagen, dissolve in deionized water at 55°C, and stir at 300 rpm for 40 minutes to obtain a mixture; d. Composition preparation: Cross-linked modified hyaluronic acid, white truffle extract, mixture, excipients and ethanol-water mixed solvent were mixed; first ultrasonic treatment (40 kHz, 250 W, 25 minutes) was performed, followed by high pressure homogenization (40 MPa, 6 cycles); pH was adjusted to 6.0, and sterilized by filtration through a 0.22 μm filter membrane to obtain the anti-aging composition.

[0022] Example 2 This embodiment provides a synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen. By weight percentage, it comprises the following components: 3% white truffle extract, 5% hydrolyzed keratin (molecular weight 800 Da), 8% soluble collagen (type I), 3% cross-linked modified hyaluronic acid, 8% glycerin, 1% ceramide NP, 1% tocopheryl acetate, 0.2% phenoxyethanol, and an ethanol-water mixed solvent (ethanol volume percentage 40%) to bring the balance to 100%. The preparation method is the same as in Example 1.

[0023] Example 3 This embodiment provides a synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen. By weight percentage, it comprises the following components: 5% white truffle extract, 6% hydrolyzed keratin (molecular weight 1500 Da), 9% soluble collagen (type I), 4% cross-linked modified hyaluronic acid, 8% glycerin, 1% ceramide NP, 1% tocopheryl acetate, 0.2% phenoxyethanol, and an ethanol-water mixed solvent (ethanol volume percentage 40%) to bring the balance to 100%. The preparation method is the same as in Example 1.

[0024] Comparative Example 1 The difference between this comparative example and Example 1 is that the anti-aging composition does not use white truffle extract. Specifically, by mass percentage, it includes the following components: hydrolyzed keratin (molecular weight 1200 Da) 7%, soluble collagen (type III) 10%, cross-linked modified hyaluronic acid 2%, glycerin 10%, ceramide NP 1%, tocopheryl acetate 1%, phenoxyethanol 0.2%, and ethanol-water mixed solvent (ethanol volume percentage 40%) to make up the balance to 100%.

[0025] The method for preparing the anti-aging composition includes the following steps: a. Preparation of cross-linked modified hyaluronic acid: Hyaluronic acid (molecular weight of 200 kDa) was first dissolved in phosphate buffer at pH 5.5-6.0, and 10% carbodiimide by weight of hyaluronic acid was added. The mixture was stirred at 30°C for 2 hours. Then, the mixture was purified by dialysis using a dialysis bag with a molecular weight cutoff of 10 kDa for 24 hours and then freeze-dried to obtain cross-linked modified hyaluronic acid. b. Mixing and dissolving: Mix hydrolyzed keratin with soluble collagen, dissolve in deionized water at 55°C, and stir at 300 rpm for 40 minutes to obtain a mixture; c. Composition preparation: Crosslinked modified hyaluronic acid, mixed solution, excipients and ethanol-water mixed solvent are mixed; first, ultrasonic treatment (40 kHz, 250 W, 25 minutes) is performed, followed by high pressure homogenization (40 MPa, 6 cycles); pH is adjusted to 6.0, and sterilized by filtration through a 0.22 μm filter membrane to obtain the anti-aging composition.

[0026] Comparative Example 2 The difference between this comparative example and Example 1 is that hydrolyzed keratin is not used in the raw materials of the anti-aging composition. Specifically, by mass percentage, it includes the following components: 4% white truffle extract, 10% soluble collagen (type III), 2% cross-linked modified hyaluronic acid, 10% glycerin, 1% ceramide NP, 1% tocopheryl acetate, 0.2% phenoxyethanol, and the balance is made up to 100% by adding an ethanol-water mixed solvent (ethanol volume percentage of 40%).

[0027] The method for preparing this anti-aging composition includes the following steps: a. Preparation of cross-linked modified hyaluronic acid: Hyaluronic acid (molecular weight of 200 kDa) was first dissolved in phosphate buffer at pH 5.5-6.0, and 10% carbodiimide by weight of hyaluronic acid was added. The mixture was stirred at 30°C for 2 hours. Then, the mixture was purified by dialysis using a dialysis bag with a molecular weight cutoff of 10 kDa for 24 hours and then freeze-dried to obtain cross-linked modified hyaluronic acid. b. Enzymatic extraction of white truffle extract: Fresh white truffles were sliced ​​and dried at 45℃ until the moisture content was ≤5%; pulverized to 150 mesh, added to a citrate buffer solution with pH 5.0, and cellulase (enzyme activity 500 U / g) was added at a material ratio of 1:50. Enzymatic hydrolysis was carried out at 52℃ for 2.5 hours; the enzyme was inactivated at 80℃, filtered, and concentrated to 1 / 3 of the volume to obtain white truffle extract; c. Mixing and dissolving: Dissolve soluble collagen in deionized water at 55°C and stir at 300 rpm for 40 minutes to obtain a mixture; d. Composition preparation: Cross-linked modified hyaluronic acid, white truffle extract, mixture, excipients and ethanol-water mixed solvent were mixed; first ultrasonic treatment (40 kHz, 250 W, 25 minutes) was performed, followed by high pressure homogenization (40 MPa, 6 cycles); pH was adjusted to 6.0, and sterilized by filtration through a 0.22 μm filter membrane to obtain the anti-aging composition.

[0028] Comparative Example 3 The difference between this comparative example and Example 1 is that soluble collagen (type III) is not used in the raw materials of the anti-aging composition. Specifically, by mass percentage, it includes the following components: 4% white truffle extract, 7% hydrolyzed keratin (molecular weight of 1200 Da), 2% cross-linked modified hyaluronic acid, 10% glycerin, 1% ceramide NP, 1% tocopheryl acetate, 0.2% phenoxyethanol, and the balance is made up to 100% by adding an ethanol-water mixed solvent (ethanol volume percentage of 40%).

[0029] The method for preparing this anti-aging composition includes the following steps: a. Preparation of cross-linked modified hyaluronic acid: Hyaluronic acid (molecular weight of 200 kDa) was first dissolved in phosphate buffer at pH 5.5-6.0, and 10% carbodiimide by weight of hyaluronic acid was added. The mixture was stirred at 30°C for 2 hours. Then, the mixture was purified by dialysis using a dialysis bag with a molecular weight cutoff of 10 kDa for 24 hours and then freeze-dried to obtain cross-linked modified hyaluronic acid. b. Enzymatic extraction of white truffle extract: Fresh white truffles were sliced ​​and dried at 45℃ until the moisture content was ≤5%; pulverized to 150 mesh, added to a citrate buffer solution with pH 5.0, and cellulase (enzyme activity 500 U / g) was added at a material ratio of 1:50. Enzymatic hydrolysis was carried out at 52℃ for 2.5 hours; the enzyme was inactivated at 80℃, filtered, and concentrated to 1 / 3 of the volume to obtain white truffle extract; c. Mixing and dissolving: Dissolve hydrolyzed keratin in deionized water at 55°C and stir at 300 rpm for 40 minutes to obtain a mixture; d. Composition preparation: Cross-linked modified hyaluronic acid, white truffle extract, mixture, excipients and ethanol-water mixed solvent were mixed; first ultrasonic treatment (40 kHz, 250 W, 25 minutes) was performed, followed by high pressure homogenization (40 MPa, 6 cycles); pH was adjusted to 6.0, and sterilized by filtration through a 0.22 μm filter membrane to obtain the anti-aging composition.

[0030] Comparative Example 4 The difference between this comparative example and Example 1 is that cross-linked modified hyaluronic acid is not used; ordinary hyaluronic acid is used, which, by mass percentage, includes the following components: 4% white truffle extract, 7% hydrolyzed keratin (molecular weight 1200 Da), 10% soluble collagen (type III), 2% hyaluronic acid, 10% glycerin, 1% ceramide NP, 1% tocopheryl acetate, 0.2% phenoxyethanol, and the balance is made up to 100% using an ethanol-water mixed solvent (ethanol volume percentage 40%).

[0031] The method for preparing this anti-aging composition includes the following steps: a. Enzymatic extraction of white truffle extract: Fresh white truffles were sliced ​​and dried at 45℃ until the moisture content was ≤5%; pulverized to 150 mesh, and citrate buffer solution with pH 5.0 was added. Cellulase (enzyme activity 500 U / g) was added at a material ratio of 1:50, and enzymatically hydrolyzed at 52℃ for 2.5 hours; the enzyme was inactivated at 80℃, filtered, and concentrated to 1 / 3 of the volume to obtain white truffle extract; b. Mixing and dissolving: Mix hydrolyzed keratin with soluble collagen, dissolve in deionized water at 55°C, and stir at 300 rpm for 40 minutes to obtain a mixture; c. Composition preparation: Hyaluronic acid, white truffle extract, mixed solution, excipients and ethanol-water mixed solvent were mixed; first ultrasonic treatment (40 kHz, 250 W, 25 minutes) was performed, followed by high pressure homogenization (40 MPa, 6 cycles); pH was adjusted to 6.0, and sterilized by filtration through a 0.22 μm filter membrane to obtain the anti-aging composition.

[0032] The present invention tested the performance of the anti-aging compositions prepared in Examples 1-3 and Comparative Examples 1-4, including skin elasticity improvement, free radical scavenging rate, collagen density increase, moisturizing duration, and storage stability. The corresponding detection methods were as follows: Skin elasticity test: the change in skin rebound rate was measured by Cutometer MPA580; Free radical scavenging rate: the scavenging rate was calculated by reacting the sample with DPPH free radical solution (0.1mM) for 30 minutes and the absorbance at 517 nm; Collagen density: the proportion of collagen area in the dermis was quantified by H&E staining of skin tissue sections and image analysis software; Moisturizing duration: the rate of skin moisture loss after application was measured by TEWL (transepidermal water loss) instrument; Storage stability: accelerated testing at 25℃ was conducted to observe changes in properties, pH, and active ingredient content.

[0033] The performance test results are shown in Table 1: Table 1 Performance Test Results

[0034] The performance test results above show that the anti-aging effects of Examples 1-3 are significant and the technical effects can be stably reproduced. In particular, the comprehensive performance of Example 1 is the most outstanding. This is mainly due to the synergistic effect of white truffle extract (antioxidant), hydrolyzed keratin (barrier repair), and soluble collagen (dermal regeneration). Through molecular complementarity, a penetration network is formed to achieve multi-target anti-aging; cross-linked hyaluronic acid maximizes the retention and stability of active ingredients.

[0035] In Comparative Example 1, without the use of white truffle extract, it can be seen that the effects of skin elasticity improvement, free radical scavenging rate, collagen density increase, moisturizing duration and storage stability are significantly reduced. This indicates that white truffle extract is the core anti-aging ingredient of this invention. Its absence will lead to a comprehensive decline in the effects of anti-oxidation, collagen regeneration and skin elasticity improvement, thus verifying the key role of white truffle in the anti-aging composition. In Comparative Example 2, without the use of hydrolyzed keratin, it can be seen that the effects of improved skin elasticity, free radical scavenging rate, increased collagen density, moisturizing duration, and storage stability are significantly reduced. This indicates that hydrolyzed keratin is a key synergistic ingredient of the present invention. Its absence leads to insufficient epidermal barrier function, thereby weakening skin elasticity and collagen regeneration effects, verifying the necessity of hydrolyzed keratin in the anti-aging system. In Comparative Example 3, without the use of soluble collagen, it can be seen that the effects of improved skin elasticity, free radical scavenging rate, increased collagen density, moisturizing duration, and storage stability are significantly reduced. This indicates that soluble collagen is the core dermal regeneration ingredient of this invention, and its absence leads to a complete collapse of collagen density and skin elasticity, verifying the key role of soluble collagen in the anti-aging composition. In Comparative Example 4, the use of hyaluronic acid instead of cross-linked hyaluronic acid resulted in a significant decrease in the effects of improved skin elasticity, free radical scavenging rate, increased collagen density, longer moisturizing duration, and storage stability. This indicates that cross-linked modified hyaluronic acid is the core excipient innovation of this invention. Its three-dimensional network structure is crucial for long-lasting moisturizing and stability. Furthermore, the active ingredients are deactivated due to the lack of protection from cross-linked hyaluronic acid, leading to a rapid deterioration in the anti-aging properties and efficacy of the product.

[0036] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen, characterized in that, By weight percentage, it includes the following components: 0.5%-5% white truffle extract, 1%-8% hydrolyzed keratin, 2%-10% soluble collagen, 2%-15% excipients, and ethanol-water mixed solvent to make up the balance to 100%.

2. The synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen according to claim 1, characterized in that, The excipients, by mass percentage, include the following components: cross-linked modified hyaluronic acid 0.5%-4%, glycerol 5%-10%, ceramide NP 0.05%-1.5%, tocopheryl acetate 0.2%-2%, and phenoxyethanol 0.1%-0.5%.

3. The synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen according to claim 2, characterized in that, The anti-aging composition comprises, by weight percentage, the following components: 4% white truffle extract, 7% hydrolyzed keratin, 10% soluble collagen, 2% cross-linked modified hyaluronic acid, 10% glycerin, 1% ceramide NP, 1% tocopheryl acetate, 0.2% phenoxyethanol, and the balance made up to 100% by an ethanol-water mixed solvent.

4. The synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen according to claim 1, characterized in that, The volume percentage of ethanol in an ethanol-water mixed solvent is 30%-50%.

5. The synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen according to claim 1, characterized in that, The cross-linked modified hyaluronic acid is prepared by cross-linking with carbodiimide, wherein the amount of carbodiimide added is 8%-12% of the mass of hyaluronic acid, and the cross-linking reaction time is 1.5-2.5 hours.

6. The synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen according to claim 1, characterized in that, The hydrolyzed keratin has a molecular weight of 800-1500 Da, and the soluble collagen is type I or type III collagen.

7. A method for preparing a synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen according to any one of claims 1-6, characterized in that, Includes the following steps: a. Preparation of cross-linked modified hyaluronic acid: Hyaluronic acid was first dissolved in phosphate buffer solution with pH 5.5-6.0, carbodiimide was added, and the mixture was stirred at 30-35℃ for 2 hours; then, after dialysis purification, it was freeze-dried to obtain cross-linked modified hyaluronic acid; b. Enzymatic extraction of white truffle extract: Fresh white truffles were sliced ​​and dried at 45℃ until the moisture content was ≤5%; after pulverization, citrate buffer solution with pH 5.0 was added, and cellulase was added at a material ratio of 1:

50. Enzymatic hydrolysis was carried out for 2.5 hours; after enzyme inactivation, the mixture was filtered and concentrated to 1 / 3 of its volume to obtain white truffle extract. c. Mixing and dissolving: Mix hydrolyzed keratin with soluble collagen, dissolve in deionized water, and stir for 40 minutes to obtain a mixture; d. Composition preparation: Cross-linked modified hyaluronic acid, white truffle extract, mixed solution, excipients and ethanol-water mixed solvent are mixed; ultrasonic treatment is performed first and then high pressure homogenization is performed; pH is adjusted to 6.0, and the mixture is sterilized by filtration through a filter membrane to obtain the anti-aging composition.

8. The method for preparing a synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen according to claim 7, characterized in that, In step a, the dialysis purification uses a dialysis bag with a molecular weight cutoff of 10 kDa, and the purification time is 24 hours.

9. The method for preparing a synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen according to claim 7, characterized in that, The viscosity of the mixture in step c is 50-100 mPa·s.

10. The method for preparing a synergistic anti-aging composition based on white truffle extract, hydrolyzed keratin, and soluble collagen according to claim 7, characterized in that, In step d, the ultrasonic treatment frequency is 40 kHz, the power is 250W, and the treatment time is 25 minutes; the high-pressure homogenization treatment pressure is 40 MPa, the number of cycles is 6, and the temperature is 55℃.

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