Anti-aging composition and application thereof as well as anti-aging freeze-dried patch and preparation method thereof

Abstract

The invention provides an anti-aging composition and application thereof, an anti-aging freeze-dried patch and a preparation method thereof. The anti-aging composition comprises an aesculus hippocastanum extract, a vaccinium macrocarpum fruit extract, a silybum marianum extract and peptides. In the anti-aging composition, the aesculus hippocastanum extract, the vaccinium macrocarpum fruit extract, the silybum marianum extract and the peptides have a synergistic effect, so that collagen synthesis can be promoted, regeneration of damaged tissues can be promoted, skin elasticity can be improved, skin wrinkles can be reduced, and a relatively good moisturizing effect can be achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care products, in particular to an anti-aging composition and application thereof, an anti-aging freeze-dried patch and a preparation method thereof. BACKGROUND

[0002] With the improvement of living standards, people pay more and more attention to the skin. Due to age, staying up late, high pressure, unhealthy diet leading to endogenous aging, and external factors such as ultraviolet radiation and long-term computer radiation, more and more skin problems such as skin wrinkles, aging, dullness, and skin sagging occur. Therefore, it is urgent to provide a skin care product with anti-aging effect. SUMMARY

[0003] Therefore, the present application provides an anti-aging composition and application thereof, and an anti-aging freeze-dried patch and a preparation method thereof, so as to achieve the anti-aging effect.

[0004] The first aspect of the present application provides an anti-aging composition comprising European horse chestnut extract, blueberry fruit extract, milk thistle extract and peptides.

[0005] In some embodiments, the anti-aging composition comprises 1-5 parts of European horse chestnut extract, 0.5-3 parts of blueberry fruit extract, 1-5 parts of milk thistle extract and 1-2 parts of peptides, by weight.

[0006] In some embodiments, the peptides comprise heptapeptide-6.

[0007] In some embodiments, the anti-aging composition further comprises sodium hyaluronate.

[0008] Optionally, the anti-aging composition comprises 2-5 parts of sodium hyaluronate, by weight.

[0009] The second aspect of the present application provides the use of the anti-aging composition of the first aspect of the present application in the preparation of a skin care product with anti-aging efficacy.

[0010] The third aspect of the present application provides an anti-aging freeze-dried patch comprising the anti-aging composition of the first aspect of the present application.

[0011] In some embodiments, the anti-aging freeze-dried patch is prepared by a freeze-drying process from an anti-aging stock solution comprising the anti-aging composition of the first aspect of the present application.

[0012] In some embodiments, the shape of the freeze-dried patch comprises at least one of a square, a rectangle, an arc, and a mask type.

[0013] The fourth aspect of the present application provides a preparation method of an anti-aging freeze-dried patch, comprising the following steps:

[0014] The anti-aging composition of the first aspect of the application is mixed with water to prepare an anti-aging stock solution;

[0015] The anti-aging stock solution is freeze-dried to prepare the anti-aging freeze-dried patch.

[0016] In some embodiments, the freeze-drying process comprises pre-freezing at -50°C for 1-2 hours, then gradually warming, vacuum control at 10-20 Pa, maintaining at -40°C to -20°C for 1-2 hours, maintaining at -20°C to -10°C for 2-4 hours, maintaining at -10°C to 1°C for 4-5 hours, until the freeze-dried water lines disappear; then maintaining at -1°C to 10°C for 4-5 hours, maintaining at 10°C to 20°C for 4-5 hours, maintaining at 20°C to 33°C for 4-5 hours; finally warming to 33°C to 35°C, vacuumizing for 1-2 hours.

[0017] The anti-aging composition provided above comprises European horse chestnut extract, blueberry fruit extract, silybum marianum extract and peptides, which four substances synergistically promote collagen synthesis, promote damaged tissue regeneration, increase skin elasticity, fade skin wrinkles, and have good moisturizing effect. DETAILED DESCRIPTION

[0018] In order to facilitate the understanding of the present application, the present application will be described more fully below with reference to relevant embodiments. The following describes the preferred embodiments of the present application. However, the present application can be implemented in many different forms, and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terminology used in the specification of the present application herein is only for the purpose of describing specific embodiments and is not intended to limit the present application.

[0020] The selection range of the terms "and / or", "or / and", "and / or" used herein includes any one of two or more related listed items, and also includes any and all combinations of related listed items, including any two related listed items, any more related listed items, or all related listed items. It should be noted that when at least two conjunctions selected from "and / or", "or / and", "and / or" are combined to connect at least three items, it should be understood that in the present application, the technical solution undoubtedly includes the technical solution connected by "logical and", and also undoubtedly includes the technical solution connected by "logical or".

[0021] In the present application, the open description of the technical features includes the closed technical solution consisting of the listed features, and also includes the open technical solution containing the listed features.

[0022] In the present application, if no special description is made, the numerical interval is considered to be continuous, and includes the minimum value and the maximum value of the range, and every value between the minimum value and the maximum value. Further, when the range refers to an integer, every integer between the minimum value and the maximum value of the range is included. In addition, when multiple ranges are provided to describe a feature or a characteristic, the ranges can be combined. In other words, unless otherwise indicated, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0023] Only some numerical ranges are specifically disclosed herein. However, any lower limit can be combined with any upper limit to form a range not explicitly recited; and any lower limit can be combined with other lower limits to form a range not explicitly recited, and likewise any upper limit can be combined with any other upper limit to form a range not explicitly recited. In addition, each individual disclosed point or single numerical value can itself be combined as a lower limit or an upper limit with any other point or single numerical value or with other lower limits or upper limits to form a range not explicitly recited.

[0024] In the present application, the temperature parameter, if not specifically limited, allows for constant temperature treatment, and also allows for treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the accuracy range controlled by the instrument. Fluctuation within a range such as ±5°C, ±4°C, ±3°C, ±2°C, ±1°C is allowed.

[0025] In the present application, the "suitable" in "suitable combination", "suitable manner", "any suitable manner" and the like means that the technical solution of the present application can be implemented, the technical problems of the present application can be solved, and the expected technical effects of the present application can be achieved.

[0026] In the present application, "further", "still further", "in particular" and the like are used for the purpose of description, indicating differences in content, but should not be understood as limiting the scope of protection of the present application.

[0027] In the present application, "optionally", "optional" and "optional" mean that it can or can not be present, i.e. it means to select either of the two parallel schemes of "yes" or "no". If there are multiple "optional" in a technical solution, unless otherwise specified, and there is no contradictory or mutual restrictive relationship, each "optional" is independent of each other.

[0028] In the description of the application, "multiple" means at least two, for example, two, three, etc., unless otherwise specifically limited.

[0029] All formulations and tests herein were conducted at ambient 25°C, unless otherwise specified.

[0030] "Comprise", "comprising", "contain", "containing", "have", "having", or any other variation thereof, are intended to cover a non-exclusive inclusion, and are not intended to exclude other steps, components, or elements not expressly stated. The terms "comprising" and "including" are used herein to mean that the named steps, components, or elements are essential, but that other steps, components, or elements which do not affect the end result can be added. The term "efficacy", "performance", "effect", "efficiency" are not distinguished between herein.

[0031] If not specified, all the embodiments and optional embodiments of the present application can be combined to form new technical solutions. If not specified, all the technical features and optional technical features of the present application can be combined to form new technical solutions.

[0032] If not specified, all the steps of the present application can be performed in sequence or randomly, preferably in sequence.

[0033] Due to age, staying up late, stress, unhealthy diet, endogenous aging, and external factors such as ultraviolet radiation and long-term computer radiation, there are more and more skin problems such as wrinkles, aging, dullness, and skin sagging.

[0034] The first aspect of the present application provides an anti-aging composition comprising European horse chestnut extract, blueberry fruit extract, milk thistle extract and peptides.

[0035] European horse chestnut extract has strong antioxidant and free radical scavenging effects, and can provide nutrition and luster to healthy skin by increasing the activity of structural proteins in the dermal-epidermal junction and the migration ability of fibroblasts. Blueberry fruit extract is rich in polyphenolic compounds such as flavonoids and anthocyanins, which have strong antioxidant effects. Antioxidants help neutralize free radicals and reduce oxidative stress damage to the body, thereby protecting cells from oxidative damage. Milk thistle extract can selectively eliminate senescent cells, promote the apoptosis of old cells, or convert the state of senescent cells to make them vibrant. Peptides can promote keratinocyte proliferation and differentiation, and repair damaged skin.

[0036] Understandably, the European horse chestnut extract, blueberry fruit extract, milk thistle extract and peptides in the anti-aging composition of the present application play a synergistic role, can promote collagen synthesis, promote regeneration of damaged tissue, increase skin elasticity, fade skin wrinkles, and have good moisturizing effect.

[0037] In some embodiments, the anti-aging composition comprises 1-5 parts by weight of the extract of European horse chestnut, 0.5-3 parts by weight of the extract of blueberry fruit, 1-5 parts by weight of the extract of silybum marianum, and 1-2 parts by weight of the peptides.

[0038] For example, the weight fraction of the extract of European horse chestnut in the anti-aging composition can be, but is not limited to, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, or a range between any two of the above weight fractions, etc.

[0039] For example, the weight fraction of the extract of blueberry fruit in the anti-aging composition can be, but is not limited to, 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, or a range between any two of the above weight fractions, etc.

[0040] For example, the weight fraction of the extract of silybum marianum in the anti-aging composition can be, but is not limited to, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, or a range between any two of the above weight fractions, etc.

[0041] For example, the weight fraction of the peptides in the anti-aging composition can be, but is not limited to, 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, or a range between any two of the above weight fractions, etc.

[0042] When the weight fractions of the extract of European horse chestnut, the extract of blueberry fruit, the extract of silybum marianum, and the peptides in the anti-aging composition are within the above ranges, respectively, the anti-aging composition can better promote collagen synthesis, promote regeneration of damaged tissues, increase skin elasticity, reduce skin wrinkles, and have better moisturizing effect.

[0043] In some embodiments, the peptides include heptapeptide-6.

[0044] As a possible embodiment, the anti-aging composition further comprises sodium hyaluronate. Since sodium hyaluronate has good moisturizing effect, the addition of sodium hyaluronate can improve the moisturizing effect of the anti-aging composition.

[0045] In some optional embodiments, the anti-aging composition comprises 2-5 parts by weight of sodium hyaluronate. For example, the anti-aging composition can contain sodium hyaluronate in a fraction of, but not limited to, 2 parts, 2.3 parts, 2.5 parts, 2.8 parts, 3 parts, 3.3 parts, 3.5 parts, 3.8 parts, 4 parts, 4.3 parts, 4.5 parts, 4.8 parts, 5 parts, or a range between any two of the above weight fractions, etc.

[0046] As a possible implementation, the anti-aging composition comprises 1-3 parts of European horse chestnut extract, 0.5-2 parts of blueberry fruit extract, 1-3 parts of silybum marianum extract, and 1-1.5 parts of heptapeptide-6, by weight.

[0047] In some embodiments, the method for preparing the anti-aging stock solution comprising the anti-aging composition described above comprises: dissolving the European horse chestnut extract, the blueberry fruit extract, the silybum marianum extract, and the heptapeptide-6 in water, stirring until clear and transparent, adding sodium hyaluronate, continuing to stir until it is uniformly dissolved, stirring until clear and transparent, to obtain the anti-aging stock solution.

[0048] The second aspect of the present application provides a use of the anti-aging composition of the first aspect of the present application in the preparation of skin care products with anti-aging efficacy.

[0049] The third aspect of the present application provides an anti-aging freeze-dried patch comprising the anti-aging composition of the first aspect of the present application. The freeze-dried patch comprising the anti-aging composition has good anti-aging and moisturizing effects.

[0050] In some embodiments, the anti-aging freeze-dried patch is prepared by a freeze-drying process from an anti-aging stock solution comprising the anti-aging composition of the first aspect of the present application.

[0051] The anti-aging freeze-dried patch of the present application is prepared by a freeze-drying process. Compared with ordinary patches, the freeze-drying process greatly ensures the stability of the active ingredients such as European horse chestnut extract, blueberry fruit extract, silybum marianum extract, and peptides. At the same time, the water-free condition is not conducive to the growth of microorganisms, thereby prolonging the shelf life of the anti-aging freeze-dried patch.

[0052] In some embodiments, the shape of the anti-aging freeze-dried patch comprises at least one of a square, a rectangle, an arc, and a mask type.

[0053] The fourth aspect of the present application provides a method for preparing an anti-aging freeze-dried patch, comprising the following steps:

[0054] mixing the anti-aging composition of the first aspect of the present application with water to prepare an anti-aging stock solution;

[0055] freeze-drying the anti-aging stock solution to prepare the anti-aging freeze-dried patch.

[0056] In some embodiments, the step of mixing the anti-aging composition of the first aspect of the present application with water to prepare an anti-aging stock solution comprises: dissolving the European horse chestnut extract, the blueberry fruit extract, the silybum marianum extract, and the heptapeptide-6 in water, stirring until clear and transparent, adding sodium hyaluronate, continuing to stir until it is uniformly dissolved, stirring until clear and transparent, to obtain the anti-aging stock solution.

[0057] In some embodiments, the freeze-drying process comprises pre-freezing at -50℃ for 1-2h, then gradually increasing the temperature, controlling the vacuum at 10-20Pa, keeping at -40℃ to -20℃ for 1-2h, keeping at -20℃ to -10℃ for 2-4h, keeping at -10℃ to 1℃ for 4-5h until the freeze-drying water lines disappear, then keeping at -1℃ to 10℃ for 4-5h, keeping at 10℃ to 20℃ for 4-5h, keeping at 20℃ to 33℃ for 4-5h, and finally increasing the temperature to 33-35℃, vacuumizing for 1-2h.

[0058] The technical solutions of the present application are described in detail below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present application and are not used to limit the scope of the present application. In the following examples, the experimental methods not specified in the specific conditions can be preferably referred to the instructions given in the present application, and can also be performed according to the experimental manuals or conventional conditions in the art, or according to the conditions suggested by the manufacturers, or according to the known experimental methods in the art.

[0059] In the following specific examples, the measurement parameters of the raw material components may have slight deviations within the weighing accuracy range if not otherwise specified. The temperature and time parameters allow for acceptable deviations caused by the instrument testing accuracy or operation accuracy.

[0060] It should be noted that the raw materials used in the above examples and comparative examples can be purchased from the market.

[0061] I. Preparation of Anti-aging Freeze-dried Patch

[0062] Example 1

[0063] Step S1. Preparation of Anti-aging Stock Solution: Dissolve 1 part of European horse chestnut extract, 1 part of Vaccinium macrocarpon fruit extract, 1 part of Silybum marianum extract, and 1 part of heptapeptide-6 in 94 parts of pure water, stir until clear and transparent, then add 2 parts of sodium hyaluronate, continue to stir until it is uniformly dissolved, and stir until clear and transparent.

[0064] Step S2. Precisely sterile filter the anti-aging stock solution obtained in step S1.

[0065] Step S3. Fill the filtered anti-aging stock solution into a mold for freeze-drying, and the freeze-drying process is as follows: pre-freeze at -50℃ for 1.5h, then gradually increase the temperature, control the vacuum at 15Pa, keep at -40℃ to -20℃ for 1.5h, keep at -20℃ to -10℃ for 3h, keep at -10℃ to 1℃ for 4h until the freeze-drying water lines disappear, then keep at -1℃ to 10℃ for 5h, keep at 10℃ to 20℃ for 4h, keep at 20℃ to 33℃ for 5h, finally increase the temperature to 35℃, vacuumize for 2h, and take out the box. The anti-aging freeze-dried patch is prepared.

[0066] Example 2

[0067] Step S1. Preparation of the anti-aging stock solution: 5 parts of European horse chestnut extract, 0.5 parts of blueberry fruit extract, 3 parts of silybum marianum extract, and 1.5 parts of heptapeptide-6 were dissolved in 85 parts of pure water, and after stirring to clarify and become transparent, 5 parts of sodium hyaluronate was added and continued to be stirred to uniformly dissolve, and stirring was continued until it became clear and transparent.

[0068] Step S2. The anti-aging stock solution obtained in step S1 was precisely sterile filtered.

[0069] Step S3. The filtered anti-aging stock solution was filled into a mold for freeze-drying, and the freeze-drying process was as follows: pre-freezing at -50℃ for 1.5 hours, then gradually warming up, vacuum control at 15 Pa, maintaining at -40℃ to -20℃ for 1.5 hours, at -20℃ to -10℃ for 3 hours, at -10℃ to 1℃ for 4 hours until the freeze-dried water lines disappeared; then maintaining at -1℃ to 10℃ for 5 hours, at 10℃ to 20℃ for 4 hours, at 20℃ to 33℃ for 5 hours; finally warming up to 35℃, vacuumizing for 2 hours, and taking out of the box. The anti-aging freeze-dried patch was prepared.

[0070] Example 3

[0071] Step S1. Preparation of the anti-aging stock solution: 3 parts of European horse chestnut extract, 3 parts of blueberry fruit extract, 5 parts of silybum marianum extract, and 2 parts of heptapeptide-6 were dissolved in 84 parts of pure water, and after stirring to clarify and become transparent, 3 parts of sodium hyaluronate was added and continued to be stirred to uniformly dissolve, and stirring was continued until it became clear and transparent.

[0072] Step S2. The anti-aging stock solution obtained in step S1 was precisely sterile filtered.

[0073] Step S3. The filtered anti-aging stock solution was filled into a mold for freeze-drying, and the freeze-drying process was as follows: pre-freezing at -50℃ for 1.5 hours, then gradually warming up, vacuum control at 15 Pa, maintaining at -40℃ to -20℃ for 1.5 hours, at -20℃ to -10℃ for 3 hours, at -10℃ to 1℃ for 4 hours until the freeze-dried water lines disappeared; then maintaining at -1℃ to 10℃ for 5 hours, at 10℃ to 20℃ for 4 hours, at 20℃ to 33℃ for 5 hours; finally warming up to 35℃, vacuumizing for 2 hours, and taking out of the box. The anti-aging freeze-dried patch was prepared.

[0074] Comparative Example 1

[0075] The preparation method of the anti-aging freeze-dried patch in Comparative Example 1 was similar to that in Example 1, except that no blueberry fruit extract, silybum marianum extract, and heptapeptide-6 were added, and the amount of European horse chestnut extract added was 4 parts.

[0076] Comparative Example 2

[0077] The preparation method of the anti-aging lyophilized patch in Comparative Example 2 is similar to that of the anti-aging lyophilized patch in Example 1, except that no European horse chestnut extract, milk thistle extract, and heptapeptide-6 are added, and the addition amount of Vaccinium macrocarpon fruit extract is 4 parts.

[0078] Comparative Example 3

[0079] The preparation method of the anti-aging lyophilized patch in Comparative Example 3 is similar to that of the anti-aging lyophilized patch in Example 1, except that no European horse chestnut extract, Vaccinium macrocarpon fruit extract, and heptapeptide-6 are added, and the addition amount of milk thistle extract is 4 parts.

[0080] Comparative Example 4

[0081] The preparation method of the anti-aging lyophilized patch in Comparative Example 4 is similar to that of the anti-aging lyophilized patch in Example 1, except that no European horse chestnut extract, Vaccinium macrocarpon fruit extract, and milk thistle extract are added, and the addition amount of heptapeptide-6 is 4 parts.

[0082] Comparative Example 5

[0083] The preparation method of the anti-aging lyophilized patch in Comparative Example 5 is similar to that of the anti-aging lyophilized patch in Example 1, except that no European horse chestnut extract is added, and the addition amounts of Vaccinium macrocarpon fruit extract, milk thistle extract, and heptapeptide-6 are 1.3, 1.3, and 1.4 parts, respectively.

[0084] Comparative Example 6

[0085] The preparation method of the anti-aging lyophilized patch in Comparative Example 6 is similar to that of the anti-aging lyophilized patch in Example 1, except that no Vaccinium macrocarpon fruit extract is added, and the addition amounts of European horse chestnut extract, milk thistle extract, and heptapeptide-6 are 1.3, 1.3, and 1.4 parts, respectively.

[0086] Comparative Example 7

[0087] The preparation method of the anti-aging lyophilized patch in Comparative Example 7 is similar to that of the anti-aging lyophilized patch in Example 1, except that no milk thistle extract is added, and the addition amounts of European horse chestnut extract, Vaccinium macrocarpon fruit extract, and heptapeptide-6 are 1.3, 1.3, and 1.4 parts, respectively.

[0088] Comparative Example 8

[0089] The preparation method of the anti-aging lyophilized patch in Comparative Example 8 is similar to that of the anti-aging lyophilized patch in Example 1, except that no heptapeptide-6 is added, and the addition amounts of European horse chestnut extract, Vaccinium macrocarpon fruit extract, and milk thistle extract are 1.3, 1.3, and 1.4 parts, respectively.

[0090] The weight part composition of the anti-aging stock solution in each of the above examples and comparative examples is shown in Table 1.

[0091] Table 1

[0092]

[0093] II. Antioxidant performance test

[0094] 1. Preparation of sample solution: 0.08 g of the product prepared in Examples 1-3 and Comparative Examples 1-8 above was weighed and added to 100 ml of distilled water to prepare a 0.8 mg / ml sample solution.

[0095] 2. Hydroxyl radical scavenging rate experiment

[0096] Reagents: 1.865 mmol / L phenanthroline anhydrous ethanol solution, 0.2 mol / L pH 7.4 phosphate buffer, 1.865 mmol / L FeSO4·7H2O solution, 0.03% (v / v) H2O2, distilled water, sample solution.

[0097] Method: 1.0 mL of 1.865 mmol / L phenanthroline anhydrous ethanol solution was taken in a stoppered test tube, 2 mL of 0.2 mol / L pH 7.4 phosphate buffer and 1 mL of sample solution were added, respectively, and mixed well. Then 1.0 mL of 1.865 mmol / L FeSO4·7H2O solution was added and mixed again. Then 1.0 mL of 0.03% (v / v) H2O2 was added, and the mixture was incubated in a 37°C constant temperature water bath. After 60 min, the absorbance value of each group of mixed solution was measured at 536 nm to obtain As. Distilled water was used instead of sample solution as a blank group to measure the absorbance value Ab. Distilled water was used instead of H2O2 as a damage group to measure the absorbance value An. The hydroxyl radical scavenging rate of the antioxidant was calculated according to the following formula: hydroxyl radical scavenging rate (%) = [(As-An) / (Ab-An)]x100. The average value of three measurements was taken, and the test results are shown in Table 2.

[0098] 3. DPPH radical scavenging rate experiment

[0099] Reagents: 0.0178 mmol / L DPPH-anhydrous methanol solution, anhydrous ethanol, 0.8 mg / ml sample solution.

[0100] Method: 3ml of 0.0178mmol / L DPPH-anhydrous methanol solution was taken in a cuvette, 3ml of sample to be tested was added, mixed immediately, and the absorbance value was measured at 517nm wavelength, and the absorbance value was recorded as Ai; the absorbance value was measured after 30min of storage at 37℃ in the dark, and the absorbance value was recorded as Aj; the blank control was only DPPH-anhydrous methanol solution without sample to be tested, mixed immediately, and the absorbance value was measured at 517nm wavelength, and the absorbance value was recorded as Ac. The DPPH free radical scavenging rate was calculated according to the following formula: DPPH free radical scavenging rate (%) = [1-(Ai-Aj) / Ac]x100%, and the average value of three measurements was taken, and the test results are shown in Table 2.

[0101] Table 2

[0102]

[0103] From the results in Table 2, compared with Comparative Examples 1-8, the anti-aging stock solution in Example 1 has a significant hydroxyl radical scavenging effect and DPPH free radical scavenging effect. The content of active ingredients in Comparative Examples 1-8 is the same as that in Example 1, but the hydroxyl radical scavenging effect and DPPH free radical scavenging effect in Comparative Examples 1-8 are significantly weaker than in Example; it is shown that European horse chestnut extract, Vaccinium macrocarpon fruit extract, Silybum marianum extract, and heptapeptide-6 have a synergistic effect, which can effectively scavenge hydroxyl radicals and DPPH free radicals.

[0104] III. Human skin test

[0105] Subject selection criteria: 352 healthy subjects were selected, and the test group was required to be 32 subjects with wrinkles, healthy skin and no damage, with an average age of 35-45 years old, randomly divided into 11 groups, and the anti-aging freeze-dried patches prepared from Examples 1-3 and Comparative Examples 1-8 were used.

[0106] Method for use: the subjects used the samples after cleansing in the morning and evening, wetted the test site with water, then applied the anti-aging freeze-dried patch to the test site, immediately removed the anti-aging patch film cloth, gently pressed until the freeze-dried eye patch was completely absorbed, and removed the patch film after 20min. During the entire test period, long-term sun exposure, outdoor exercise, travel, etc. should not be performed, and during the entire test period, no cosmetics or drugs with similar efficacy to the sample should be used, and the subjects should not change their daily care habits during the test period.

[0107] The subjects were tested after 0 weeks and 4 weeks of continuous use of the test sample, and the test results were the average values of 32 subjects in each group.

[0108] Elasticity test:

[0109] Skin elasticity was tested by using the skin elasticity tester MPA580 of Germany CK company, and the cheek part was tested by using the Reviscometer RV600 probe, and the results are shown in Table 3.

[0110] Table 3

[0111]

[0112] From the results in Table 3, it can be seen that compared with Comparative Example 1-8, the skin elasticity of the test site was significantly improved after using the anti-aging freeze-dried patch in Example 1 for 28 days. The content of active ingredients in Comparative Example 1-8 is the same as that in Example 1, but the effect in Comparative Example 1-8 is significantly worse than that in Example 1; indicating that European horse chestnut extract, Vaccinium macrocarpon fruit extract, Silybum marianum extract, and Heptapeptide-6 have a synergistic effect, which can further improve skin elasticity.

[0113] Wrinkle improvement

[0114] The measuring instrument of wrinkle area ratio is facial image analyzer VISIA-CR, and the test area is the corner of the eye. The data in Table 4 are calculated. The greater the absolute value of the change rate, the better the wrinkle improvement effect.

[0115] Table 4

[0116]

[0117] From the results in Table 4, it can be seen that compared with Comparative Example 1-8, the wrinkles of the test site were significantly improved after using the anti-aging freeze-dried patch in Example 1 for 28 days. The content of active ingredients in Comparative Example 1-8 is the same as that in Example 1, but the wrinkle improvement effect in Comparative Example 1-8 is significantly weaker than that in Example 1; indicating that European horse chestnut extract, Vaccinium macrocarpon fruit extract, Silybum marianum extract, and Heptapeptide-6 have a synergistic effect, which can further improve skin elasticity.

[0118] Moisturizing efficacy test

[0119] The test environment is a room with temperature: 25.0℃±1.0℃; humidity: 50%±10%, and the test personnel sit still for 30 min. The stratum corneum water content of the test area is tested by using MoistureMeter SC, and the results are shown in Table 5. The trans-epidermal water loss (TEWL) of the test area is tested by using VapoMeter, and the results are shown in Table 6.

[0120] Table 5 Skin moisture content (%) at different times (h)

[0121]

[0122] Table 6 Results of skin transepidermal water loss test

[0123]

[0124] From the results of Table 5 and Table 6, it can be seen that the moisturizing effect of the anti-aging freeze-dried patch in Examples 1-3 is better than that of Comparative Examples 1-8; it is shown that the anti-aging freeze-dried patch provided in the present application can significantly increase the skin water content, make the skin persistent water-locking, and has good moisturizing effect.

[0125] In summary, the anti-aging freeze-dried patch containing European horse chestnut extract, blueberry fruit extract, silybum marianum extract, heptapeptide-6 and sodium hyaluronate provided in the present application can promote collagen synthesis, promote damaged tissue regeneration, increase skin elasticity, and fade skin wrinkles, and has good moisturizing effect.

[0126] The technical features of the above-described embodiments can be combined arbitrarily, and to make the description concise, all possible combinations of the technical features in the above-described embodiments are not described, however, as long as the combinations of the technical features do not exist contradictions, they should be considered as the scope of the present disclosure.

[0127] The above-described embodiments only express several embodiments of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent. It should be noted that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are within the scope of the present application. Therefore, the scope of the patent protection of the present application should be subject to the appended claims.

Claims

1. An anti-aging composition, characterized in that, It contains extracts of European horse chestnut, blueberry fruit, milk thistle, and peptides.

2. The anti-aging composition according to claim 1, characterized in that, The anti-aging composition comprises, by weight, 1 to 5 parts of European horse chestnut extract, 0.5 to 3 parts of blueberry fruit extract, 1 to 5 parts of milk thistle extract, and 1 to 2 parts of peptides.

3. The anti-aging composition according to claim 1, characterized in that, The peptides include heptapeptide-6.

4. The anti-aging composition according to any one of claims 1 to 3, characterized in that, The anti-aging composition also contains sodium hyaluronate; Optionally, the anti-aging composition comprises 2 to 5 parts by weight of sodium hyaluronate.

5. The use of an anti-aging composition as described in any one of claims 1 to 4 in the preparation of a skin care product having anti-aging effects.

6. An anti-aging freeze-dried patch, characterized in that, Includes the anti-aging composition as described in any one of claims 1 to 4.

7. The anti-aging freeze-dried patch as described in claim 6, characterized in that, The anti-aging freeze-dried patch is prepared by freeze-drying an anti-aging stock solution containing the anti-aging composition as described in any one of claims 1 to 4.

8. The anti-aging freeze-dried patch as described in any one of claims 6 to 7, characterized in that, The shape of the anti-aging freeze-dried patch includes at least one of square, rectangular, arc, and mask type.

9. A method for preparing an anti-aging freeze-dried patch as described in any one of claims 6 to 8, characterized in that, Includes the following steps: The anti-aging composition according to any one of claims 1 to 4 is mixed with water to prepare an anti-aging stock solution; The anti-aging stock solution was freeze-dried to prepare the anti-aging freeze-dried patch.

10. The preparation method according to claim 9, characterized in that, The freeze-drying process includes: pre-freezing at -50℃ for 1-2 hours, then gradually increasing the temperature while maintaining a vacuum of 10Pa-20Pa, holding at -40℃--20℃ for 1-2 hours, holding at -20℃--10℃ for 2-4 hours, and holding at -10℃-1℃ for 4-5 hours until the freeze-dried watermarks disappear; then holding at -1℃-10℃ for 4-5 hours, holding at 10℃-20℃ for 4-5 hours, and holding at 20℃-33℃ for 4-5 hours; finally, increasing the temperature to 33℃-35℃ and evacuating the vacuum for 1-2 hours.

Images