Total-effect itching-relieving skin care composition and application thereof

Abstract

The invention relates to a skin care composition capable of fully relieving itching and application thereof, the skin care composition comprises the following components: kelp extract, kadsura longepedunculata extract, andrographis paniculata leaf extract and roselle flower extract, and the weight ratio of the kelp extract to the kadsura longepedunculata extract to the andrographis paniculata leaf extract to the roselle flower extract is (0.01-3): (0.01-3): (0.1-5): (0.1-3). The four components cooperate with one another in multiple dimensions of skin barrier repair, nerve regulation, anti-inflammation, micro-ecological regulation, oxidation resistance and the like, and the synergistic itching relieving effect is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to a skin care composition for relieving itching and application thereof. BACKGROUND

[0002] Facial itching as a common skin problem has complex causes, mainly including three types. Skin barrier damage is the main cause of skin-derived itching. When the skin barrier structure is damaged due to excessive cleaning, dry environment or improper skin care, its water retention capacity decreases, leading to increased water loss, dry skin and desquamation, directly stimulating exposed nerve endings to produce itching, which is usually accompanied by obvious skin dryness, tightness and even desquamation. Neurogenic itching is caused by abnormal activation of the sensory nervous system or dysfunction of the conduction pathway. Its characteristics are that the itching may suddenly occur, the position is not fixed or wandering, and the skin surface is often without primary skin lesions, but emotional fluctuations, temperature changes and dietary stimuli can easily aggravate it. Microecology-related itching is mainly triggered by skin flora imbalance. For example, when Staphylococcus aureus proliferates excessively, it can secrete protease V8, which can directly activate the PAR1 receptor on the skin nerve fiber, thereby bypassing the traditional inflammatory pathway to directly transmit the itch signal to the brain. At the same time, flora imbalance can damage the normal biological barrier function, exacerbate inflammation and skin barrier damage, forming a vicious cycle of "the more you scratch, the more it itches".

[0003] Most of the anti-itch products on the market use a single mechanism solution: one is to use antihistamines such as diphenhydramine and steroids to temporarily suppress inflammation, but long-term use can cause skin atrophy and dependence; two is to use nerve modulators, which can block the transmission of itch signals to some extent, but often have no inhibitory and improving effect on inflammation and barrier; three is to use antibacterial ingredients to regulate the microecology, but broad-spectrum antibacterial agents will indiscriminately kill beneficial bacteria and harmful bacteria, disrupting the balance of the microecosystem. These traditional methods have obvious defects. Single-target treatment cannot cover the multiple pathological mechanisms of itching, and it only treats the symptoms but not the root cause. Secondly, chemically synthesized ingredients can easily cause skin irritation and tolerance problems. Furthermore, current measures to relieve itching ignore the vicious cycle of pathogenic bacteria such as Staphylococcus aureus and itching. Research by Cell has confirmed that Staphylococcus aureus directly induces itching through the TSLP pathway. SUMMARY

[0004] The present application relates to the technical field of cosmetics, in particular to a skin care composition for relieving itching and application thereof.

[0005] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows:

[0006] In a first aspect, the present application provides a skin care composition with full-effect anti-itching, comprising the following components: a giant kelp extract, a schisandra chinensis extract, a justicia gendarussa leaf extract, and a Hibiscus sabdariffa flower extract, wherein the weight ratio of the giant kelp extract, the schisandra chinensis extract, the justicia gendarussa leaf extract, and the Hibiscus sabdariffa flower extract is (0.01-3):(0.01-3):(0.1-5):(0.1-3).

[0007] The components of the skin care composition of the present application have the following effects:

[0008] The fucoidan contained in the giant kelp extract forms a biomimetic moisturizing film on the skin surface, reducing transdermal water loss, while the brown algae polyphenol protects type IV collagen and laminin from degradation by inhibiting the activity of matrix metalloproteinase MMP-9, thereby strengthening the basement membrane zone structure. This mechanism is particularly suitable for repairing the damaged skin barrier due to excessive cleaning or external stimulation, creating a favorable environment for subsequent active ingredient penetration.

[0009] The schisandra chinensis extract exerts a dual effect of nerve regulation and anti-inflammation through its core components schizandrin A and schizandrin B. Schizandrin A can effectively inhibit the activity of TRPV1 channels on sensory neurons, reducing the intensity of itch signal transmission; at the same time, by reducing the release of substance P and histamine, it raises the itch threshold. Schizandrin B, on the other hand, regulates the NF-κB inflammatory pathway, inhibiting TNF-α, IL-31, and other pruritogenic factors, thereby reducing the stimulation of nerve endings by inflammation from the source.

[0010] The active ingredient andrographolide in the justicia gendarussa leaf extract has a microecological intelligent regulation function, specifically inhibiting the quorum sensing system of Staphylococcus aureus, reducing the stimulation of delta-toxin produced by bacteria on keratinocytes; on the other hand, it promotes the growth of beneficial bacteria Staphylococcus epidermidis, increasing its secreted sphingomyelinase activity, and enhancing ceramide synthesis. More importantly, andrographolide can block the thymic stromal lymphopoietin (TSLP) signaling pathway induced by Staphylococcus aureus, fundamentally breaking the vicious cycle of microecological imbalance and itch formation.

[0011] The rose flower extract is rich in high-activity anthocyanins and natural polyphenol compounds. Its unique small molecular structure enables it to quickly penetrate into the deep keratin layer. By eliminating oxygen free radicals and inhibiting lipid peroxidation, it effectively blocks the continuous stimulation of oxidative stress on nerve endings. At the same time, the natural polysaccharide ingredients in the extract form a breathable biological protective film on the skin surface, significantly improving dry pruritus caused by impaired barrier function. More importantly, the quercetin derivatives in the rose flower extract can synergistically enhance the inhibitory effect of the schisandra extract on the TRPV1 channel, and regulate the activity of transient receptor potential ankyrin 1 (TRPA1) to bidirectionally inhibit the itch signal transduction pathway. The contained chlorogenic acid can also promote keratinocytes to secrete β-defensin, selectively inhibit the growth of pathogenic bacteria without disrupting the balance of beneficial bacteria, and consolidate the skin defense system from the microecological level.

[0012] Preferably, the weight ratio of the giant kelp extract, the schisandra extract, the andrographis extract, and the rose flower extract is (0.05-1.5):(0.05-2):(0.5-3):(0.5-2).

[0013] Preferably, the weight ratio of the giant kelp extract, the schisandra extract, the andrographis extract, and the rose flower extract is (0.5-1):(0.1-1):(1-2):(1-1.5).

[0014] In a second aspect, the present application provides use of the skin care composition of the first aspect in the preparation of a cosmetic product.

[0015] Preferably, the cosmetic product is a cosmetic water, emulsion, cream, mask, serum, or spray, and the skin care composition is added in an amount of 0.5%-5% of the total weight of the cosmetic product.

[0016] In a third aspect, the present application provides a serum, comprising the following raw materials in the following weight percentages: 0.5%-5% of the skin care composition of the first aspect, 0.05%-0.5% of a thickening agent, 0.5%-10% of a moisturizing agent, 0.5%-5% of a preservative, and 0.01%-0.3% of a pH regulator, and the balance being deionized water.

[0017] Preferably, the thickening agent comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / acryloyl dimethyl taurate sodium copolymer, acryloyl dimethyl ammonium taurate / VP copolymer, and arthrobacter gum.

[0018] Preferably, the moisturizing agent comprises at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerol, D-panthenol, and ceramide.

[0019] Preferably, the pH regulator comprises at least one of arginine, tromethamine and disodium EDTA.

[0020] Preferably, the preservative comprises at least one of 1,3-propanediol, 1,2-hexanediol and p-hydroxyacetophenone.

[0021] In a fourth aspect, the present application provides a preparation method of the serum in the third aspect, characterized in that comprising the following steps:

[0022] S1, mixing the humectant, the thickening agent and part of the deionized water, homogenizing at 75-85 DEG C, then adding the preservative, stirring uniformly to obtain a mixture;

[0023] S2, after the temperature of the mixture in S1 drops to 35-45 DEG C, adding each component in the skin care composition and the remaining deionized water, stirring uniformly, and finally adding the pH regulator to adjust the pH to obtain the serum.

[0024] Compared with the prior art, the present application has the following beneficial effects:

[0025] The brown algal polyphenol and fucoidan rich in the giant kelp extract in the skin care composition of the present application have excellent antioxidant and barrier repair functions; the lignan compounds in the Schisandra chinensis, such as schizandrin A and B, can inhibit the release of inflammatory mediators and regulate nerve sensitivity; andrographolide in the Andrographis herb extract has been proved to selectively inhibit Staphylococcus aureus without affecting the beneficial Staphylococcus epidermidis, while reducing the expression of inflammatory factors such as IL-1β and IL-6. The rose bengal flower extract provides strong antioxidant protection by its rich anthocyanins and gallic acid, scavenging free radicals and reducing nerve ending sensitization caused by oxidative stress, while its polysaccharide component forms a water-retaining film on the skin surface, enhancing the barrier function. The giant kelp extract, the Schisandra chinensis extract, the Andrographis herb extract and the rose bengal flower extract cooperate with each other in multiple dimensions such as skin barrier repair, nerve regulation, anti-inflammatory, microecological regulation and antioxidant through their unique mechanisms of action, achieving the effect of synergistic itching relief. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 The figure is a zebrafish neutrophil inflammation map for Example 1 and the control group. DETAILED DESCRIPTION

[0027] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples.

[0028] The raw materials used in the following examples and comparative examples are as follows:

[0029] Sargassum extract: manufacturer Guangzhou Haishi Fine Chemical Co., Ltd., trade name SEACTIVE TM Algae amoxicillin;

[0030] Rose flower extract: manufacturer GREENTECH, model / tradename FLOWER

[0031] Schisandra chinensis extract: manufacturer Draco, trade name Schisandra Chinensis Extract

[0032] Andrographis panniculata leaf extract: manufacturer Nanchang Biotechnology Co., Ltd., trade name SupraBiome TM AP.

[0033] Other materials, reagents, etc. used in the examples can be obtained from commercial channels unless otherwise specified.

[0034] Examples 1-7 and Comparative Examples 1-4

[0035] The composition components (mass ratio) of the all-purpose anti-itch skin care compositions of Examples 1-7 and Comparative Examples 1-4 are shown in Table 1. The total mass parts of each group of compositions are 100 parts.

[0036] The preparation method of the all-purpose anti-itch skin care compositions of Examples 1-7 and Comparative Examples 1-4 includes the following steps:

[0037] Each component is stirred and mixed uniformly at a rotation speed of 300 rpm to obtain the all-purpose anti-itch skin care composition.

[0038] Table 1 Formulation of each group of all-purpose anti-itch skin care compositions

[0039]

[0040] Test Example 1: Inflammation inhibition test of the composition

[0041] Neutrophils are the first white blood cells to arrive at the site of inflammation in the immune system, responsible for phagocytosis and clearance of harmful substances released by pathogens and damaged tissues. When inflammation occurs, neutrophils will rapidly migrate to the damaged area and aggregate, and the number change can directly reflect the severity of inflammation. The neutrophils of zebrafish are highly similar to humans in morphology, biochemistry and function, so by observing the dynamic changes of zebrafish neutrophils, the process of human inflammatory response can be accurately simulated. Inflammation is one of the important factors leading to itching, and by detecting the size of the inhibitory effect of the composition on inflammation, the strength of the anti-itch effect of the composition can be obtained.

[0042] 1) Preparation of experimental materials

[0043] Biological sample: healthy zebrafish developed for 5 days after fertilization (screening criteria: normal morphology and development to the blastula stage).

[0044] Reagents:

[0045] Zebrafish culture solution: 2940 mg of anhydrous calcium chloride, 1233 mg of magnesium sulfate heptahydrate, 630 mg of sodium bicarbonate, 55 mg of potassium chloride, and 1 g of methyl blue were dissolved in 10 L of water. The stimulant was sodium dodecyl sulfate (60 μg / mL). All chemicals were of analytical grade.

[0046] Zebrafish culture solution containing test samples: the zebrafish culture solution was diluted to a sample solution of 2 wt% for the full-effect antipruritic compositions of Examples 1-7 and Comparative Examples 1-4.

[0047] 2) Experimental grouping

[0048] Healthy zebrafish developed for 5 days after fertilization were selected and grouped and operated as follows:

[0049] Blank group: 200 μL of zebrafish culture solution was added to each well;

[0050] Control group: 10 μL of sodium dodecyl sulfate (60 μg / mL) was injected into the tail fin of the zebrafish before culture, and 200 μL of zebrafish culture solution was added to each well during culture;

[0051] Sample group: 10 μL of sodium dodecyl sulfate (60 μg / mL) was injected into the tail fin of the zebrafish before culture, and 200 μL of zebrafish culture solution containing 2 wt% test sample was added to each well during culture.

[0052] 3) Culture conditions

[0053] The blank group, control group, and sample group were placed in a 24-well plate for culture, with at least 10 zebrafish in each group. After the healthy zebrafish were treated according to the above grouping conditions, they were placed in a constant-temperature incubator and cultured at 28°C for 3 h.

[0054] 4) Data collection and analysis

[0055] Fluorescence imaging: 10 zebrafish in each group were placed under a fluorescence microscope to take fluorescence images of the dorsal region of the zebrafish.

[0056] Neutrophil quantification: the data were analyzed and collected using advanced image processing software ImageJ, the target area was accurately delineated using the rectangle tool, and the average fluorescence intensity of the selected area was measured using the gray intensity method, i.e., the number of neutrophils in the zebrafish.

[0057] 5) Calculation of the inhibition rate of neutrophils in zebrafish

[0058] The specific data are shown in Table 2.

[0059] Test Example 2: Pruritogenic Factor TSLP Inhibition Test

[0060] Staphylococcus aureus can induce thymic stromal lymphopoietin (TSLP) signaling pathway, triggering a vicious cycle of skin microecological imbalance and pruritus formation. By detecting the size of the inhibition of TSLP by the composition, the strength of the antipruritic effect of the composition can be obtained.

[0061] The cell line used is human keratinocyte HaCaT (BeNa Biotech), and the test conditions are: incubator temperature 37±1℃, humidity 90±5 RH%, carbon dioxide 5±1% v / v; cell culture and treatment are carried out according to the grouping, followed by testing, and the specific test is TSLP content detection, the test method is as follows:

[0062] (1) The cell suspension was inoculated in a 96-well cell culture plate, with a density of 2000 cells per well, and 100 μL of culture medium was added to each well, and cultured for 24 h;

[0063] (2) Discard the supernatant, add 100 μL of DMEM medium containing 10 μg / mL of LPS (2105341, Gibco) to the control group, and add 100 μL of DMEM medium containing 10 μg / mL of LPS and 0.1% of the full-effect antipruritic composition prepared in Examples 1-7 or Comparative Examples 1-4 to the sample group;

[0064] (3) After 24 h of incubation, the culture medium and cells were collected respectively;

[0065] (4) TSLP detection: The TSLP content in keratinocytes was detected according to the experimental steps of the human thymic stromal lymphopoietin (TSLP) ELISA kit (Enzymedynamics, EH0322). The standard and the appropriately diluted sample to be tested were added to the corresponding wells, and after incubation, the unbound components were washed away. Biotin-detection antibody was added, and the biotin-detection antibody combined with TSLP that had been bound to the coated antibody. After washing away the unbound components, HRP-streptavidin (SABC) was added. After washing away the unbound components, TMB color developing substrate was added, which turned blue under the catalysis of horseradish peroxidase (HRP), and turned yellow after the addition of reaction termination solution. The OD value was measured at 450 nm wavelength with a microplate reader. The concentration of TSLP in the sample was calculated by drawing a standard curve, and the calculation formula of TSLP inhibition rate is as follows:

[0066] TSLP inhibition rate (%) = (control group content - sample group content) / control group content x 100%, and the specific data is shown in Table 2.

[0067] Table 2: Neutrophil inhibition rate and TSLP inhibition rate of each sample

[0068] Group / Performance Neutrophil inhibition rate / % TSLP inhibition rate / % Example 1 72.3 89.3 Example 2 68.4 85.3 Example 3 71.9 87.9 Example 4 62.0 82.8 Example 5 64.8 80.3 Example 6 58.1 73.3 Example 7 59.8 76.1 Comparative Example 1 45.4 56.2 Comparative Example 2 51.4 59.0 Comparative Example 3 54.8 63.2 Comparative Example 4 48.7 67.5

[0069] Depend on Figure 1 It can be seen that the dorsal region of the zebrafish treated with the composition of Example 1 has a smaller proportion of fluorescence compared with the control group, indicating that the number of neutrophils in the zebrafish treated with the composition of Example 1 is smaller. Example 1 has a higher inhibition rate of neutrophils in zebrafish and a better anti-inflammatory effect.

[0070] The sum of Example 1 and Comparative Examples 1-4 in Table 2 Figure 1 Data shows that the absence of any one of the following extracts—Kelp extract, Schisandra chinensis extract, Andrographis paniculata leaf extract, and Roselle flower extract—reduces the neutrophil inhibition rate and TSLP inhibition rate of the composition, indicating that these four components have a synergistic effect in inhibiting inflammation and pruritus.

[0071] As can be seen from the data in Examples 1 and 2-7 in Table 2, when the weight ratio of giant kelp extract, Schisandra chinensis extract, Andrographis paniculata leaf extract and roselle flower extract is (0.5-1):(0.1-1):(1-2):(1-1.5), the antipruritic and anti-inflammatory effects of the composition are at a better level.

[0072] Application Example 1-7 and Comparative Application Example 1-4

[0073] The all-purpose antipruritic compositions of Examples 1-7 and Comparative Examples 1-4 were added to the serum at a concentration of 3 wt% to obtain the serums of Application Examples 1-7 and Comparative Application Examples 1-4. The formulations are shown in Table 3.

[0074] The preparation methods of the serums used in Application Examples 1-7 and Comparative Application Examples 1-4 include the following steps:

[0075] The preparation method of the serum includes the following steps:

[0076] S1. Mix the humectant, thickener and 1 / 2 volume of deionized water, homogenize at 80°C, then add the preservative and stir until homogeneous to obtain the mixture.

[0077] S2. Once the temperature of the mixture in S1 drops to 40°C, add all components of the all-in-one anti-itch skincare composition and the remaining deionized water, stir well, and finally add a pH adjuster to adjust the pH to 6.5 to obtain the essence.

[0078] Table 3. Serum formulations for Application Examples 1-7 and Comparative Application Examples 1-4.

[0079]

[0080]

[0081] Comparative Application Example 5

[0082] The essence of Comparative Application Example 5 does not add the full-effect antipruritic composition, and an equal amount of deionized water is used instead of the composition, and the preparation method is the same as Application Example 1.

[0083] Test Example 3: Instant itch relief and long-term moisturizing, soothing, and improving microecological effects of the essence

[0084] Subject criteria: The volunteers were included according to the general provisions of the safety test of the Cosmetics Safety Technical Specifications, and 48 Asian adult test users aged 18-60 years who felt that their skin was sensitive were selected for the test, with a lactic acid stinging score of ≥3 points, and were randomly divided into 12 groups, with 4 people in each group.

[0085] Patch test (closed type): 48 hours before all subjects were enrolled, normal skin areas (avoiding scars and pigmentation) on both sides of the spine on the back were used to apply standard 10-hole patch devices (Beijing Baiyi Yida) with samples containing ① the essence of Application Examples 1-7 and Comparative Examples 1-5, ② negative control (normal saline), and ③ positive control (0.1 wt% benzophenone-3), with a distance of ≥1 cm between each point. After 24 hours of application, the patches were removed, and skin reactions were observed at 30 minutes, 24 hours, and 48 hours after removal, respectively. The reactions were scored according to the standards of “no reaction (0), slight erythema (1), obvious erythema + edema (2), and blisters (3)”. Subjects with a score of ≤1 were enrolled, and those with a score of ≥2 were directly excluded from the group.

[0086] Acute irritation screening: For subjects who passed the patch test, 0.1 g of the essence to be tested was applied to a small piece of normal skin (1 cm x 1 cm) on the face in front of the ear twice a day for 3 consecutive days. Subjects with no redness, swelling, or stinging were included in the efficacy test.

[0087] Steps for instant itch improvement test: After each subject applied a 10% lactic acid solution to the nasolabial fold of the face (diameter 0.8 cm double-layer filter paper) for 5 minutes, the induced facial itching was removed, and a questionnaire was used to evaluate the self-itch score. Then, 50 μg of the essence containing the examples and comparative examples was applied, and the facial itching caused by lactic acid was scored within 1 minute after application. The subjects' itching sensation was evaluated using a 4-point scale, and the questionnaire content was: What is your current facial itching score? 0 - no sensation, 1 - mild itching, 2 - obvious itching, 3 - severe itching. The calculation formula for the instant itch improvement rate is as follows:

[0088] Instant itch improvement rate (%) = (initial score - score after use) / initial score x 100%; see Table 4 for specific data.

[0089] Procedure of long-lasting barrier repair and moisturizing test: On the day of the visit, the volunteers washed their faces with water in the test area without applying any product, and sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 min. The Tewameter™ Hex probe was used to test the TEWL and the water content of the cheekbone area. CM 825 probe was used to test the TEWL and the water content of the cheekbone area. The subjects applied the serum twice a day, once in the morning and once in the evening, each time 1 pump, about 1 g, and returned for a visit after 28 days, and the TEWL and water content of the cheekbone area were tested. The improvement rate of TEWL value and the improvement rate of water content were as follows:

[0090] TEWL value improvement rate (%) = |TEWL value 使用后 -TEWL value 使用前 | / TEWL value 使用前 × 100%;

[0091] Water improvement rate (%) = |water 使用后 - water 使用前 | / water 使用前 × 100%; see Table 4 for specific data.

[0092] The improvement effect of the application example on the barrier is represented by the TEWL improvement rate, and the improvement effect of the application example on the water is represented by the water improvement rate.

[0093] Table 4 Test results of test example 3 of the serum of each group

[0094] Group / Performance Lactic acid itch improvement rate / % TEWL value improvement rate / % Moisture improvement rate / % Application Example 1 90.9 23.0 45.3 Application Example 2 81.8 20.6 42.8 Application Example 3 77.8 20.2 43.7 Application Example 4 81.8 18.9 38.4 Application Example 5 80.0 19.5 41.6 Application Example 6 72.7 15.7 32.6 Application Example 7 77.8 15.4 31.4 Comparative Application Example 1 63.6 7.1 15.0 Comparative Application Example 2 54.5 9.7 19.2 Comparative Application Example 3 50.0 11.0 23.4 Comparative Application Example 4 46.5 12.5 26.3 Comparative Application Example 5 40.3 2.3 4.3

[0095] Test example 4: improvement of facial microecology effect test of serum

[0096] The subjects were randomly selected from the application examples and the comparative application examples of the serum of test example 3 above and the blank serum group.

[0097] Facial microorganism collection:

[0098] 1) Preparation: sterile swab, sterile cryogenic tube, marker pen, sampling liquid (sterile physiological saline).

[0099] 2) Sampling: the sampling site was the left cheekbone area, and a 2 cm x 2 cm square area was marked,

[0100] If the area has acne, scars, replace the right side of the cheekbone symmetrical area; take a sterile swab, immerse it in a cryopreservation tube containing 0.5 mL of sterile saline, gently squeeze the swab rod (remove excess liquid, make sure the swab is wet but not dripping, the squeezing force should be appropriate so that the swab head is not deformed; use a sterile forceps to fix the middle of the swab rod, completely stick the swab head to the skin in the delineated area, keep the swab rod at a 45-degree angle with the skin, draw a circle clockwise, speed 1 circle / 2 seconds, last 30 seconds (15 circles), during which rotate the swab rod every 10 seconds (5 circles) 1 time (clockwise rotation 90 degrees, make sure each side of the swab head contacts the skin), take 3 swabs for each sample;

[0101] 3) Cryopreservation sample detection: After wiping, cut or break off the cotton swab head, place it in a sterile cryopreservation tube, tightly cap and seal, immediately transport to the laboratory for microbiome testing. Extract total bacterial DNA from the sample according to the procedure of the Bacterial Genomic DNA Extraction Kit (Tiangen, DP302), and strictly control its purity. The extracted DNA is subjected to random fragmentation, end repair and adapter ligation to construct a standardized sequencing library. Subsequently, high-throughput sequencing platforms such as Illumina NovaSeq are used for sequencing by synthesis to generate double-end read data. The raw data obtained needs to be filtered by quality control, removing low-quality sequences and adapter sequences, then using BLAST in NCBI (https: / / blast.ncbi.nlm.nih.gov / Blast.cgi) for species identification, obtaining the total number of strains on the face of the subject, then calculating the number of strains of each different species, calculating the percentage of Staphylococcus aureus and the percentage of Staphylococcus epidermidis on the face of the subject, and the distribution of Staphylococcus aureus / Staphylococcus epidermidis on the face of each subject is calculated, the formula is as follows:

[0102] The percentage of different species (%) = (total number of skin strains - number of strains of each species) / number of strains of each species x 100%. The lower the number of Staphylococcus aureus / Staphylococcus epidermidis on the face of the subject, the more Staphylococcus epidermidis, the less harmful Staphylococcus aureus, the more stable the skin microecology, the data is shown in Table 5. Table 5 Test results of sterilization effect of each group

[0103]

[0104]

[0105] It can be seen from Tables 4-5 that, according to the data of application example 1 and comparative application examples 1-4 in Table 4, the lactic acid itching improvement rate, repair, moisturizing and pathogenic bacteria removal effect of the serum of application example 1 using the composition containing the extract of giant kelp, the extract of Schisandra chinensis, the extract of Andrographis herb and the extract of Hibiscus sabdariffa flower are all significantly improved compared with comparative application examples 1-4 lacking one of the above components, which shows that the above four components cooperate with each other in multiple dimensions such as skin barrier repair, nerve regulation, anti-inflammatory, microecological regulation and antioxidant, and achieve the synergistic itching-relieving effect.

[0106] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A skin care composition for full efficacy in relieving itching, characterized by comprising: The composition comprises the following components: giant kelp extract, schisandra chinensis extract, andyrianthi leaf extract, and hibiscus sabdariffa flower extract, the weight ratio of the giant kelp extract, the schisandra chinensis extract, the andyrianthi leaf extract, and the hibiscus sabdariffa flower extract is (0.01-3):(0.01-3):(0.1-5):(0.1-3).

2. The full-effect antipruritic skin care composition of claim 1, wherein, The weight ratio of the giant kelp extract, the schisandra chinensis extract, the andyrianthi leaf extract, and the hibiscus sabdariffa flower extract is (0.05-1.5):(0.05-2):(0.5-3):(0.5-2).

3. The full-effect antipruritic skin care composition of claim 1, wherein, The weight ratio of the giant kelp extract, the schisandra chinensis extract, the andyrianthi leaf extract, and the hibiscus sabdariffa flower extract is (0.5-1):(0.1-1):(1-2):(1-1.5).

4. Use of the skin care composition of any one of claims 1-3 in the preparation of a cosmetic product.

5. Use of the full-effect antipruritic skin care composition according to claim 4 for the preparation of a cosmetic product, characterized in that, The cosmetic product is a cosmetic water, emulsion, cream, mask, serum, or spray, and the skin care composition is added in an amount of 0.5%-5% by weight of the total weight of the cosmetic product.

6. An essence characterized by, The composition comprises the following raw materials: 0.5%-5% by weight of the skin care composition of any one of claims 1-3, 0.05%-0.5% by weight of a thickening agent, 0.5%-10% by weight of a humectant, 0.5%-5% by weight of a preservative, and 0.01%-0.3% by weight of a pH adjuster, and the balance is deionized water.

7. The serum of claim 6, wherein the serum is a serum for skin care. The raw materials are selected from at least one of (a)-(d): (a) the thickening agent comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, and sclerotium gum; (b) the humectant comprises at least one of allantoin, betaine, beta-glucan, trehalose, glycerol, D-panthenol, and ceramide; (c) the pH adjuster comprises at least one of arginine, tromethamine, and disodium EDTA; (d) the preservative comprises at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

8. The method of claim 6, wherein the serum is prepared by the steps of: The method comprises the following steps: S1, mixing the humectant, the thickening agent, and part of the deionized water, homogenizing at 75-85°C, then adding the preservative and stirring until uniform to obtain a mixture; S2, after the temperature of the mixture in S1 drops to 35-45°C, adding the components of the skin care composition and the remaining deionized water, stirring until uniform, and finally adding the pH adjuster to adjust the pH to obtain the serum.

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