PSEP detection kit convenient to operate

Abstract

The invention provides a PSEP detection kit convenient to operate, and relates to the field of protein detection. The outer wall of the kit main body is a heat-insulating material, and the inner wall of the kit main body is a heat-conducting material; the reagent support frame is mounted in the kit main body; the microwell plate is mounted in the kit main body; the liquid preparation tank is arranged in the kit main body; the ventilation holes are uniformly formed in the front end surface and the rear end surface of the kit main body; the ventilation opening and closing assembly is arranged on the inner side of the inner wall of the kit main body; and four kit supporting legs are fixedly connected to the bottom of the kit main body. The PSEP detection kit is simple in structure and convenient to use, reagents are convenient to prepare and take, operation convenience is improved, temperature requirements of different scenes are met, operation is easy, use is convenient, and the problems that when an existing PSEP detection kit is used, disordered placement is prone to occurring, carrying is inconvenient, an ice bag easily dissipates temperature, and the detection effect is affected are solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of protein detection, in particular to a PSEP detection kit convenient to operate. BACKGROUND

[0002] In the diagnosis process of prostate diseases, the proteins excreted by prostate corpuscles generally need to be detected, and PSEP detection is generally used. PSEP detection is generally realized by using a PSEP detection kit. At present, the PSEP detection kits on the market generally include a kit main body, reagent bottles, a microplate and other basic components, and some kits are equipped with simple storage structures to place detection-related equipment. However, when the existing PSEP detection kit is used, the detection personnel generally place the reagent bottles, the microplate and the liquid preparation container according to their own habits, which easily leads to a messy arrangement, resulting in complicated reagent taking and transferring operations and reducing the detection efficiency. Meanwhile, when the PSEP detection kit needs to be carried over a long distance, an ice bag generally needs to be provided, but the ice bag is inconvenient to carry and easily loses temperature, which affects the detection effect. SUMMARY

[0003] Therefore, the present application provides a PSEP detection kit convenient to operate, which realizes the orderly placement of the microplate, the liquid preparation tank and the reagent bottles, facilitates reagent preparation and taking, improves the operation convenience, the kit main body is made of a material combining heat insulation and heat conduction, the ice bag seat can maintain a low temperature, the ventilation opening state can be flexibly controlled by the ventilation opening and closing assembly, the temperature requirements of different scenes can be met, the ice bag seat is stably installed through the linkage of the rotary knob supporting legs, and the ventilation state is adjusted during installation, which is simple to operate and convenient to use.

[0004] The present application provides a PSEP detection kit convenient to operate, which realizes the orderly placement of the microplate, the liquid preparation tank and the reagent bottles, facilitates reagent preparation and taking, improves the operation convenience, the kit main body is made of a material combining heat insulation and heat conduction, the ice bag seat can maintain a low temperature, the ventilation opening state can be flexibly controlled by the ventilation opening and closing assembly, the temperature requirements of different scenes can be met, the ice bag seat is stably installed through the linkage of the rotary knob supporting legs, and the ventilation state is adjusted during installation, which is simple to operate and convenient to use. The outer wall of the kit main body is made of a heat insulation material, and the inner wall of the kit main body is made of a heat conduction material. The kit cover is hingedly connected to the top of the kit main body. The ice bag seat is installed at the bottom of the kit main body through the installation assembly. The reagent support frame is installed inside the kit main body. The microplate is installed inside the kit main body. The liquid preparation tank is arranged inside the kit main body. The front and rear end faces of the kit main body are uniformly arranged with ventilation holes. The ventilation opening and closing assembly is arranged on the inner side of the inner wall of the kit main body. Four kit supporting legs are fixedly connected to the bottom of the kit main body.

[0005] Furthermore, a well plate mounting slot is provided on the top left side of the reagent kit body, and a solution preparation mounting slot is provided on the top right side of the reagent kit body. The microplate is installed inside the well plate mounting slot, and the solution preparation slot is installed inside the solution preparation mounting slot. Multiple solution preparation slots are provided.

[0006] Furthermore, the reagent support frame has multiple reagent mounting holes in the middle, the size of which is adapted to the reagent bottle.

[0007] Furthermore, the installation components include: The knob-operated support legs are connected to the four corners of the bottom of the ice pack holder by rotation. The knob leg has a locking thread at the top, and the reagent kit leg has a hollow structure with an internal thread at the bottom of the inner hole. The knob leg is screwed onto the bottom of the reagent kit leg through the locking thread.

[0008] Furthermore, the mounting components also include: The synchronous sprocket and the knob support leg are coaxially fixedly connected to one of the four synchronous sprockets, which are connected by a transmission chain to form a transmission chain transmission mechanism.

[0009] Furthermore, the ventilation opening and closing assembly includes: There are four intermediate sliding frames, which are slidably connected to the four corners of the reagent kit body. The guide slide is located on the side of the middle sliding frame. The middle sliding frame is slidably connected to the reagent kit body through the guide slide. A spring is installed on the top of the middle sliding frame, and the middle sliding frame is elastically connected to the reagent kit body through the spring.

[0010] Furthermore, the ventilation opening and closing assembly also includes: The drive rod is fixedly connected to the bottom of the intermediate sliding frame and slides inside the reagent kit legs.

[0011] Furthermore, the ventilation opening and closing assembly also includes: The sealing plate is slidably connected to the inner sidewalls of the front and rear sides of the reagent kit body; The sealing plate has a guide hole at each of its four corners. The sealing plate is slidably connected to the main body of the reagent kit through the guide holes. A spring is set at each of the four outer corners of the sealing plate. The sealing plate is elastically connected to the main body of the reagent kit through the spring.

[0012] Furthermore, the ventilation opening and closing assembly also includes: A drive wedge is fixedly connected to both the upper and lower sides of the middle sliding frame, with the inclined surface of the drive wedge facing upwards. The driven wedge is fixedly connected to each of the four inner corners of the sealing plate. The inclined surface of the driven wedge faces downward. The driven wedge and the inclined surface of the driven wedge cooperate to form a wedge structure.

[0013] Furthermore, sealing blocks are evenly arranged and fixedly connected to the outer side of the sealing plate. The sealing blocks are conical structures with smaller outer sides and larger inner sides, and the ventilation holes are conical hole structures with larger inner sides and smaller outer sides. The sealing blocks and the conical surfaces of the ventilation holes are matched.

[0014] Beneficial effects This invention utilizes the design of a well plate mounting slot, a solution preparation mounting slot, and a reagent support frame to achieve orderly placement of microplates, solution preparation tanks, and reagent bottles, facilitating reagent preparation and retrieval, and improving operational convenience. The main body of the reagent kit is made of a material that combines heat insulation and thermal conductivity, and can maintain low temperatures when used with an ice pack holder. The ventilation opening and closing component can flexibly control the ventilation hole status to meet the temperature requirements of different scenarios. The ice pack holder is securely installed via a linked knob support leg, and the ventilation status can be adjusted during installation. The operation is simple and convenient.

[0015] This invention provides a microplate mounting slot and a solution preparation slot within the main body of the reagent kit. These slots can hold microplates and multiple solution preparation slots, respectively. The solution preparation slots can be specifically configured with different reagents such as enzyme-labeled reagents and cleaning agents. This makes it easier to inject solutions into the microplates using a multi-channel pipette. The reagent mounting holes on the reagent support rack can accommodate various reagent bottles, enabling centralized storage and retrieval of detection-related reagents and reducing reagent searching and transfer steps during operation.

[0016] The main body of the reagent kit of this invention is designed with heat-insulating outer wall and heat-conducting inner wall. Combined with the ice pack holder that can be installed at the bottom, the internal low temperature environment can be effectively maintained by the ice pack during the carrying process, so as to avoid the reagent from being affected by temperature fluctuations. The ventilation opening and closing component can flexibly control the state of the ventilation holes according to the usage scenario: when carrying, the ventilation holes are blocked to enhance heat insulation; when placed in a refrigerator or when room temperature equilibrium is required, the ventilation holes are opened to ensure heat exchange between the inside and the outside, so as to meet different temperature requirements.

[0017] The ice pack holder of this invention is installed by threaded connection between the knob legs and the reagent kit legs. The four sets of knob legs are linked by a synchronous sprocket and a transmission chain, and rotating any one set will operate synchronously, making installation and disassembly efficient and the connection stable. In the ventilation opening and closing assembly, the sealing block and the ventilation hole adopt a conical surface fit, which can reliably realize the sealing and opening of the ventilation hole. Each sliding part is elastically connected by a spring to ensure the stability of the structure and improve the overall reliability of use. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings of the embodiments will be briefly described below.

[0019] The accompanying drawings described below are only related to some embodiments of the invention and are not intended to limit the invention.

[0020] In the attached diagram: Figure 1 This is a schematic diagram of the overall structure of an easy-to-use PSEP detection kit according to an embodiment of the present invention.

[0021] Figure 2 This is a schematic diagram of the PSEP test kit with the lid removed, which is an embodiment of the present invention.

[0022] Figure 3 This is a schematic diagram of the exploded structure of the easy-to-operate PSEP detection kit of the present invention with the kit lid removed.

[0023] Figure 4 This is a schematic diagram of the bottom structure of an easy-to-use PSEP test kit according to an embodiment of the present invention.

[0024] Figure 5 This is a schematic diagram of the structure of the easy-to-use PSEP test kit of the present invention in the state after removing the ice pack holder.

[0025] Figure 6 This is a schematic diagram of the ice pack holder structure of an easy-to-use PSEP test kit according to an embodiment of the present invention.

[0026] Figure 7 This is a schematic diagram of the ventilation opening and closing component structure of an easy-to-operate PSEP test kit according to an embodiment of the present invention.

[0027] Figure 8 This is a cross-sectional structural diagram of an easy-to-use PSEP detection kit according to an embodiment of the present invention.

[0028] Figure 9 This is an isometric sectional view of an easy-to-use PSEP detection kit according to an embodiment of the present invention.

[0029] List of reference numerals 1. Reagent kit body; 101. Ventilation hole; 102. Plate mounting slot; 103. Solution preparation mounting slot; 104. Reagent kit support leg; 2. Reagent kit lid; 3. Ice pack holder; 4. Reagent support frame; 401. Reagent mounting hole; 5. Microplate; 6. Solution preparation tank; 7. Knob support leg; 701. Synchronous sprocket; 702. Locking thread; 8. Intermediate sliding frame; 801. Drive wedge; 802. Guide groove; 803. Drive rod; 9. Sealing plate; 901. Driven wedge; 902. Sealing block; 903. Guide hole. Detailed Implementation

[0030] Example 1: Please refer toFigures 1 to 6 As shown: This invention provides an easy-to-use PSEP detection kit, comprising a kit body 1; The outer wall of the reagent kit body 1 is made of heat-insulating material, and the inner wall of the reagent kit body 1 is made of heat-conducting material; The reagent kit lid 2 is hinged to the top of the reagent kit body 1; Ice pack holder 3 is installed at the bottom of the reagent kit body 1 via an installation component; Reagent support frame 4 is installed inside the reagent kit body 1; Microplate 5 is installed inside the reagent kit body 1; The solution preparation tank 6 is located inside the main body 1 of the reagent kit. The reagent kit body 1 has a plate mounting slot 102 on the top left side and a solution preparation mounting slot 103 on the top right side. The microplate 5 is installed inside the plate mounting slot 102, and the solution preparation tank 6 is installed inside the solution preparation mounting slot 103. There are multiple solution preparation tanks 6. In use, multiple solution preparation tanks 6 can be used to prepare enzyme-labeled reagents, cleaning agents, colorimetric agents and other reagents respectively. At the same time, it is convenient to inject the reagents into the microplate 5 through a multi-channel pipette, which improves the convenience of use.

[0031] The reagent support frame 4 has multiple reagent mounting holes 401 in the middle. The size of the reagent mounting holes 401 is adapted to the reagent bottles. In use, buffer reagent bottles, enzyme labeling reagent bottles, colorimetric reagent bottles, washing solution reagent bottles, stop solution reagent bottles and other reagent bottles are placed through the reagent mounting holes 401.

[0032] The installation components include: Knob support leg 7, which is rotatably connected to the four bottom corners of ice pack base 3; The top of the knob support leg 7 is provided with a locking thread 702. The reagent kit support leg 104 is a hollow structure. The bottom of the inner hole of the reagent kit support leg 104 is provided with an internal thread. The knob support leg 7 is tightened to the bottom of the reagent kit support leg 104 through the locking thread 702, so as to realize the installation of the ice pack holder 3 and the reagent kit body 1.

[0033] The installation components also include: The synchronous sprocket 701 and the knob support leg 7 are coaxially fixedly connected in the middle. The four synchronous sprockets 701 are connected by a transmission chain to form a transmission chain transmission mechanism. In use, when any set of knob support legs 7 is rotated, the transmission chain transmission mechanism realizes the synchronous rotation of the four sets of knob support legs 7.

[0034] The specific usage and function of this embodiment: When using this invention, a microplate 5 is placed through the plate mounting slot 102, and a solution preparation tank 6 is placed through the solution preparation mounting slot 103. As needed, enzyme-labeled reagents, cleaning agents, colorimetric agents, and other reagents are prepared in multiple solution preparation tanks 6. Then, the reagents are injected into the microplate 5 using a pipette for PSEP detection.

[0035] Example 2: Please refer to Figures 7 to 9 As shown: This invention provides an easy-to-use PSEP test kit, including ventilation holes 101, with ventilation holes 101 evenly distributed on both the front and rear ends of the kit body 1. Ventilation opening and closing component, which is located on the inner side of the inner wall of the reagent kit body 1; Four reagent kit legs 104 are fixedly connected to the bottom of the main body 1 of the reagent kit.

[0036] The ventilation opening and closing components include: The intermediate sliding frame 8 consists of four pieces, which are slidably connected to the four corners of the reagent kit body 1. The guide slide 802 is located on the side of the intermediate sliding frame 8. The intermediate sliding frame 8 is slidably connected to the reagent kit body 1 through the guide slide 802. A spring is provided on the top of the intermediate sliding frame 8, and the intermediate sliding frame 8 is elastically connected to the reagent kit body 1 through the spring.

[0037] The ventilation opening and closing components also include: The drive rod 803 is fixedly connected to the bottom of the intermediate sliding frame 8. The drive rod 803 slides inside the reagent kit support leg 104. During use, when the knob support leg 7 is tightened, the knob support leg 7 pushes the drive rod 803 upward, and the drive rod 803 drives the intermediate sliding frame 8 to compress the spring and slide upward.

[0038] The ventilation opening and closing components also include: The sealing plate 9 is slidably connected to the inner sidewalls of the front and rear sides of the reagent kit body 1; The sealing plate 9 has a guide hole 903 at each of its four corners. The sealing plate 9 is slidably connected to the reagent kit body 1 through the guide holes 903. A spring is provided at each of the four outer corners of the sealing plate 9. The sealing plate 9 is elastically connected to the reagent kit body 1 through the springs.

[0039] The ventilation opening and closing components also include: A drive wedge 801 is fixedly connected to both the upper and lower sides of the middle sliding frame 8, with the inclined surface of the drive wedge 801 facing upwards. Driven wedge 901: Each of the four inner corners of the sealing plate 9 is fixedly connected to a driven wedge 901. The inclined surface of the driven wedge 901 faces downward. The driven wedge 801 and the inclined surface of the driven wedge 901 cooperate to form a wedge structure. In use, when the intermediate sliding frame 8 slides upward, the intermediate sliding frame 8 drives the sealing plate 9 to slide outward through the wedge structure formed by the driven wedge 801 and the driven wedge 901.

[0040] Among them, sealing blocks 902 are evenly arranged and fixedly connected on the outer side of the sealing plate 9. The sealing block 902 is a conical structure with a smaller outer side and a larger inner side. The ventilation hole 101 is a conical hole structure with a larger inner side and a smaller outer side. The sealing block 902 and the conical surface of the ventilation hole 101 are matched. In use, when the sealing block 902 is inserted into the ventilation hole 101, the ventilation hole 101 is sealed through the conical surface matching, so as to achieve heat insulation between the main body of the reagent kit 1 and the outside world.

[0041] The specific usage and function of this embodiment are as follows: When remote carrying is required, place an ice pack in the ice pack holder 3, place the reagent kit body 1 on top of the ice pack holder 3, insert the locking thread 702 into the reagent kit support leg 104, rotate any set of knob support legs 7, and achieve synchronous rotation of the four sets of knob support legs 7 through the transmission chain mechanism. Tighten the locking thread 702 into the reagent kit support leg 104, and then tighten the four knob support legs 7 one by one to ensure good thread strength. The ice pack holder 3 and the bottom of the reagent kit body 1 are pressed together, realizing the connection between the reagent kit body 1 and the ice pack holder 3. The ice pack holder 3 is installed; at the same time, during the tightening of the knob support leg 7, the knob support leg 7 pushes the drive rod 803 upward, the drive rod 803 drives the intermediate sliding frame 8 to compress the spring and slide upward, the intermediate sliding frame 8 drives the sealing plate 9 to slide outward through the wedge structure composed of the drive wedge 801 and the driven wedge 901, the sealing block 902 is inserted into the ventilation hole 101, and the ventilation hole 101 is sealed by the conical surface, so as to achieve heat insulation between the reagent kit body 1 and the outside world. At this time, the ice pack is used to cool the inside of the reagent kit body 1 to prevent the temperature from being too high; When placed in a refrigerator or for room temperature equalization, loosen the knob support leg 7, remove the ice pack holder 3, and the middle sliding frame 8 and sealing plate 9 will be reset under the action of the spring. The sealing block 902 will disengage from the ventilation hole 101, and the ventilation hole 101 will open, thereby realizing heat exchange between the inside of the reagent kit body 1 and the outside.

Claims

1. An easy-to-use PSEP detection kit, characterized in that, include: The main body of the reagent kit (1); The outer wall of the reagent kit body (1) is made of heat-insulating material, and the inner wall of the reagent kit body (1) is made of heat-conducting material; The reagent kit lid (2) is hinged to the top of the reagent kit body (1); Ice pack holder (3), which is mounted on the bottom of the kit body (1) via a mounting assembly; A reagent support frame (4) is installed inside the reagent kit body (1); Microplate (5), the microplate (5) is installed inside the reagent kit body (1); A solution preparation tank (6) is disposed inside the main body (1) of the reagent kit; Ventilation holes (101) are evenly arranged on the front and rear ends of the reagent kit body (1). Ventilation opening and closing component, wherein the ventilation opening and closing component is disposed on the inner side of the inner wall of the reagent kit body (1); The bottom of the reagent kit body (1) is fixedly connected to four reagent kit legs (104).

2. The easy-to-use PSEP test kit as described in claim 1, characterized in that: The reagent kit body (1) has a plate mounting slot (102) on the top left side and a solution preparation mounting slot (103) on the top right side. The microplate (5) is installed inside the plate mounting slot (102) and the solution preparation tank (6) is installed inside the solution preparation mounting slot (103). The solution preparation tank (6) has multiple components.

3. The easy-to-use PSEP test kit as described in claim 1, characterized in that: The reagent support frame (4) has multiple reagent mounting holes (401) in the middle, and the size of the reagent mounting holes (401) is adapted to the reagent bottle.

4. The easy-to-use PSEP test kit as described in claim 1, characterized in that: The installation components include: Knob legs (7) are rotatably connected to the four bottom corners of the ice pack holder (3); Locking thread (702): The top of the knob leg (7) is provided with locking thread (702). The reagent kit leg (104) is a hollow structure. The bottom of the inner hole of the reagent kit leg (104) is provided with internal thread. The knob leg (7) is screwed onto the bottom of the reagent kit leg (104) through locking thread (702).

5. The easy-to-use PSEP test kit as described in claim 4, characterized in that: The installation components also include: Synchronous sprocket (701), one of which is coaxially fixedly connected to the middle of the knob support leg (7), and the four synchronous sprockets (701) are connected by a transmission chain to form a transmission chain transmission mechanism.

6. The easy-to-use PSEP test kit as described in claim 1, characterized in that: The ventilation opening and closing assembly includes: The intermediate sliding frame (8) is provided in four parts, and the four intermediate sliding frames (8) are slidably connected to the four corners of the reagent kit body (1); The guide slide (802) is located on the side of the intermediate sliding frame (8). The intermediate sliding frame (8) is slidably connected to the reagent kit body (1) through the guide slide (802). A spring is provided on the top of the intermediate sliding frame (8). The intermediate sliding frame (8) is elastically connected to the reagent kit body (1) through the spring.

7. The easy-to-use PSEP test kit as described in claim 6, characterized in that: The ventilation opening and closing assembly also includes: A drive rod (803) is fixedly connected to the bottom of the intermediate sliding frame (8) and slides inside the reagent kit support leg (104).

8. The easy-to-use PSEP test kit as described in claim 7, characterized in that: The ventilation opening and closing assembly also includes: The sealing plate (9) is slidably connected to the inner side of the front and rear side walls of the reagent kit body (1); Guide hole (903): A guide hole (903) is provided at each of the four corners of the sealing plate (9). The sealing plate (9) is slidably connected to the reagent kit body (1) through the guide hole (903). A spring is provided at each of the four outer corners of the sealing plate (9). The sealing plate (9) is elastically connected to the reagent kit body (1) through the spring.

9. The easy-to-use PSEP detection kit as described in claim 8, characterized in that: The ventilation opening and closing assembly also includes: A drive wedge (801) is fixedly connected to both the upper and lower sides of the intermediate sliding frame (8), with the inclined surface of the drive wedge (801) facing upward. Driven wedge (901): A driven wedge (901) is fixedly connected to each of the four inner corners of the sealing plate (9). The inclined surface of the driven wedge (901) faces downward. The driven wedge (801) and the inclined surface of the driven wedge (901) cooperate to form a wedge structure.

10. The easy-to-use PSEP detection kit as described in claim 9, characterized in that: The sealing plate (9) is uniformly arranged and fixedly connected with sealing blocks (902). The sealing blocks (902) are cone structures with smaller outer sides and larger inner sides. The ventilation holes (101) are cone structures with larger inner sides and smaller outer sides. The sealing blocks (902) and the ventilation holes (101) are in contact with the cone surfaces of the sealing blocks (902) and the ventilation holes (101).

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