Microbial fermentation device with sealing detection function

Abstract

The invention relates to the technical field of microbial fermentation, and discloses a microbial fermentation device with a sealing detection function, the microbial fermentation device comprises a base, one side of the top of the base is fixedly connected with a monitoring box, the other side of the top of the base is fixedly connected with a plurality of groups of supporting legs, and the middle parts of the plurality of groups of supporting legs are provided with a fermentation tank; a cooling interlayer is fixedly connected to the outer side of the fermentation tank, and a bearing sleeve is fixedly connected to the middle of the top end of the fermentation tank. A driving motor is started to drive a first rotating shaft to rotate, after the first rotating shaft rotates, a first crushing plate and a second crushing plate are driven to rotate through a series of transmission, bubbles are scattered and changed into a plurality of small bubbles, meanwhile, due to shearing of the first crushing plate and the second crushing plate, sterile air can be further subdivided, and the air quality is improved. The sterile air can be in better contact with the liquid culture medium, so that the gas-liquid contact specific surface area can be obviously increased, and the oxygen dissolution is accelerated.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of microbial fermentation, and particularly relates to a microbial fermentation device with a sealing detection function. BACKGROUND

[0002] The microbial fermentation device realizes sufficient contact between a substrate and a bacterial body by constructing a closed and sterile environment, precisely controlling parameters such as temperature, pH and dissolved oxygen, and combining mechanical stirring or gas circulation to drive efficient synthesis of microbial metabolites.

[0003] However, the existing microbial fermentation device still has some problems in actual use. First, the fermentation process of the fermentation device is extremely strict, and the inside of the fermentation device needs to be completely sealed. If the sealing is not strict, microorganisms in the air are likely to enter the inside of the fermentation device, thereby invading the fermentation system and competing with beneficial bacteria for nutrients, resulting in failure of fermentation. Second, the existing microbial fermentation device introduces sterile gas into the inside of the tank during the working process, so that the microorganisms inside are in an active state. In the prior art, the sterile gas is directly introduced into the inside of the tank through a pipeline. After the gas enters the inside of the tank, large bubbles are formed. The large bubbles contact the liquid medium, thereby fusing the oxygen in the sterile air into the liquid medium. However, the contact area of the bubbles with the liquid is small, and the oxygen in the sterile air cannot be well fused into the inside of the liquid medium. The bubbles continuously rise, so that the oxygen content at the top of the tank is too high, and the oxygen content at the bottom of the tank is low, thereby causing uneven distribution of the oxygen content, which may affect the microbial fermentation, thereby reducing the working efficiency. SUMMARY

[0004] The application provides a microbial fermentation device with a sealing detection function, which has the advantages of quickly fusing oxygen into the inside of a liquid medium, so as to solve the problem of uneven distribution of oxygen.

[0005] To achieve the above object, the application adopts the following technical scheme: a microbial fermentation device with a sealing detection function, comprising a base, one side of the top of the base is fixedly connected with a monitoring box, the other side of the top of the base is fixedly connected with a plurality of supporting legs, the middle parts of the plurality of supporting legs are provided with fermentation tanks, the outer sides of the fermentation tanks are fixedly connected with cooling interlayers, and the middle parts of the top ends of the fermentation tanks are fixedly connected with bearing sleeve pipes. The top of the bearing sleeve pipe is fixedly connected with a driving motor, the output end of the driving motor is fixedly connected with a first rotating shaft, the outer side of the first rotating shaft is movably sleeved with a mounting sleeve, and the inside of the mounting sleeve is provided with a coaxial reverse rotation assembly. The inside of the fermenter is fixedly connected with a first bottom plate, the inside of the first bottom plate is provided with a smashing assembly, the outside of the first rotating shaft is provided with a plurality of stirring assemblies; The outside of the first rotating shaft is fixedly connected with a mounting block, the inside of the mounting block is provided with a scraping assembly; The outside of the top of the fermenter is movably sleeved with a monitoring ring, the inside of the monitoring ring is provided with a monitoring assembly.

[0006] Preferably, the coaxial reverse assembly comprises a driving gear fixedly sleeved on the outside of the first rotating shaft, a plurality of driven gears meshingly connected on the outside of the driving gear, a second rotating shaft fixedly connected in the plurality of driven gears, the second rotating shaft rotatably connected with the mounting sleeve, a gear ring fixedly connected in the mounting sleeve, the gear ring meshingly connected with the plurality of driven gears, a triangular block movably sleeved on the outside of the second rotating shaft, a connecting shaft fixedly connected on one side of the middle of the triangular block, the connecting shaft movably sleeved on the outside of the first rotating shaft, a fixed rod fixedly connected on one side of the mounting sleeve, the fixed rod fixedly connected with the fermenter, and the connecting shaft rotatably connected with the mounting sleeve.

[0007] Preferably, the smashing assembly comprises a first air outlet plate fixedly sleeved on the outside of the connecting shaft, a second air outlet plate provided on the lower end of the first air outlet plate, the second air outlet plate fixedly sleeved on the outside of the first rotating shaft, and a plurality of air outlet holes formed in the middle of the first air outlet plate and the second air outlet plate.

[0008] Preferably, the smashing assembly further comprises a plurality of first smashing plates fixedly connected to the lower surface in the first air outlet plate, a plurality of second smashing plates fixedly connected to the top of the second air outlet plate, the teeth of the first smashing plate and the second smashing plate are staggered, a gas guide hole formed in the inside of the first bottom plate, and a sterile air inlet fixedly connected on one side of the bottom of the fermenter, the sterile air inlet in communication with the gas guide hole, so as to facilitate the gas to be delivered to the inside of the fermenter.

[0009] Preferably, the stirring assembly comprises a fixed plate, and a plurality of impellers fixedly connected on the outside of the fixed plate.

[0010] Preferably, the scraping assembly comprises a breaking column fixedly connected to the inside of the mounting block, a cleaning sleeve column movably sleeved on the outside of the breaking column, a scraper fixedly connected in the cleaning sleeve column, the inner ring of the scraper abutting against the outer surface of the breaking column, a pushing block fixedly connected to the top of the cleaning sleeve column, one side of the pushing block fixedly connected with one end of a first spring, and the other end of the first spring fixedly connected with the mounting block.

[0011] Preferably, the scraping assembly further comprises a variable speed bearing fixedly sleeved on the outside of the first rotating shaft, the outside of the variable speed bearing is fixedly connected with a cam, the cam and the push block are arranged on the same axis, and the cam and the push block are equal in height.

[0012] Preferably, the monitoring assembly comprises an upper top plate and a lower bottom plate, the upper top plate and the lower bottom plate are fixedly connected to the upper surface inside the monitoring ring and the lower surface inside the monitoring ring respectively, the inner side surface of the upper top plate is provided with a plurality of first sliding grooves, the inside of each of the plurality of first sliding grooves is slidably connected with a connecting block, one side of the connecting block is fixedly connected with a moving column, the outside of the upper top plate is movably sleeved with a moving plate, and the other side of the connecting block is fixedly connected with the moving plate.

[0013] Preferably, the monitoring assembly further comprises a plurality of second sliding grooves, the plurality of second sliding grooves are all arranged in the inside of the lower bottom plate, one end of a second spring is fixedly connected to the inside of each of the plurality of second sliding grooves, the other end of the second spring is fixedly connected with a connecting column, the connecting column is slidably connected with the second sliding groove, the connecting column is fixedly connected with the moving plate, and the width of the lower bottom plate is equal to the sum of the upper top plate and the moving plate.

[0014] Preferably, the top side of the fermenter is fixedly connected with a feeding pipe, one side of the cooling layer is fixedly connected with a cooling water inlet, the other side of the cooling layer is fixedly connected with a cooling water outlet, the bottom of the fermenter is fixedly connected with a discharging outlet, one side of the discharging outlet is fixedly connected with a steam inlet, one side of the cooling layer is fixedly connected with a window, the top of the fermenter is fixedly connected with an exhaust port, and the cooling layer is fixedly connected with the supporting leg.

[0015] The beneficial effects of the present application are as follows: The application drives the first rotating shaft to rotate by the driving motor, the first rotating shaft drives the driving gear to rotate, the driving gear drives the driven gear to rotate along the gear ring, the driven gear drives the second rotating shaft and the triangular block to rotate, the triangular block drives the connecting shaft to rotate, the connecting shaft rotates to make the first air outlet plate rotate, at this time, the rotation of the first rotating shaft drives the second air outlet plate to rotate, the rotation direction of the connecting shaft and the first rotating shaft is changed due to the wheel system inside the mounting sleeve, so that the first air outlet plate and the second air outlet plate rotate in opposite directions, realize coaxial reverse rotation, after the sterile air enters the inside of the first air outlet plate and the second air outlet plate, the rotating first air outlet plate and the second air outlet plate drive the first crushing plate and the second crushing plate to crush them, so that they become a plurality of small bubbles, at the same time, due to the staggered arrangement of the first crushing plate and the second crushing plate, the bubbles in the first air outlet plate and the second air outlet plate will be sheared during rotation, so that the bubbles are further cut and scattered, and they become smaller bubbles, thereby significantly increasing the gas-liquid contact specific surface area, accelerating oxygen dissolution, at the same time, the rotation direction of the second air outlet plate and the first air outlet plate is opposite, which forms a two-way stirring flow field, eliminates the liquid laminar flow dead angle, ensures uniform distribution of oxygen, and guarantees efficient dispersion of sterile air, maximization of gas-liquid mass transfer and optimization of mixing uniformity, improves fermentation efficiency, reduces energy consumption and cost, and guarantees process stability.

[0016] The first rotating shaft can also drive the fixed plate and the impeller to rotate, the microorganism fermentation is stirred, so that the microorganism fermentation process maintains a suspended state, avoids sediment accumulation, ensures uniform distribution of bacteria and sufficient contact with culture medium, thereby accelerating the metabolic reaction rate of substrate and microorganism, the stirring can also promote gas-liquid mass transfer at the same time, increase oxygen dissolution efficiency (especially aerobic fermentation) by breaking bubbles, and improve temperature, pH and uniformity of nutrients, prevent local environmental differences from causing fermentation efficiency to decrease. In addition, stirring can also reduce the risk of clumping in the liquid medium, maintain fluidization, promote timely diffusion of metabolites, reduce the probability of growth of miscellaneous bacteria, and improve overall fermentation stability and product yield.

[0017] The application drives the mounting block and the breaking column to rotate through the first rotating shaft, and the breaking column mechanically defoams the foam generated in the microbial reaction process after rotating, the surface of the breaking column is rich in water after multiple contacts with the foam, and the elimination efficiency of the foam is reduced due to the rich water on the surface when the foam is eliminated by impact, and the rotating speed of the cam is slower than the rotating speed of the mounting block driven by the first rotating shaft due to the setting of the variable speed bearing, at this time, the cam is in contact with the pushing block, the pushing block is pushed out, the cleaning sleeve column and the top of the scraper are driven to scrape the hydration layer attached to the surface of the breaking column, the water in the foam film is reduced, the direct action of the defoaming device is enhanced, the defoaming efficiency is reduced due to the water layer, and the hydration layer can also cause corrosion or scaling on the surface of the breaking column, the scraping action of the breaking column can prolong the service life of the equipment and reduce the maintenance cost, and the pushing block is reset by the first spring after the cam rotates away, and the cycle work is realized.

[0018] When the monitoring ring is arranged at the connection of the fermentation tank, the edge of the fermentation tank lifts the moving column, the moving column drives the connecting block and the moving plate to lift, so that the inside of the monitoring ring is opened, the water flow in the monitoring ring liquid seals the connection of the fermentation tank, the connection can be monitored in real time by the monitoring ring during the working process, the risk of leakage is avoided, the system safety is improved, the deformation of the connection is self-adapted by the water flow pressure, and the sealing and monitoring functions are integrated. BRIEF DESCRIPTION OF DRAWINGS

[0019] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the present disclosure and, together with the description, serve to explain the principles of the present disclosure.

[0020] The present disclosure can be more clearly understood and appreciated from the following detailed description, taken in conjunction with the following drawings, in which: Figure 1 It is a schematic diagram of the overall structure of the present application; Figure 2 It is an internal view of the fermentation tank of the present application; Figure 3 It is a schematic diagram of the structure of the first rotating shaft of the present application; Figure 4 It is an internal view of the mounting sleeve of the present application; Figure 5 It is a schematic diagram of the structure of the triangular block of the present application; Figure 6 It is a schematic diagram of the breaking assembly structure of the present application; Figure 7 It is a comparison diagram of the first gas outlet plate and the second gas outlet plate of the present application; Figure 8 It is a schematic diagram of the internal structure of the mounting block of the present application; Figure 9 This is a schematic diagram of the cam structure of the present invention; Figure 10 This is a schematic diagram of the detection component structure of the present invention; Figure 11 This is a schematic diagram of the structure of the second spring of the present invention.

[0021] The components include: 1. Base; 2. Monitoring box; 3. Support leg; 4. Fermentation tank; 5. Cooling jacket; 6. Bearing sleeve; 7. Drive motor; 8. First rotating shaft; 9. Mounting sleeve; 10. Drive gear; 11. Driven gear; 12. Second rotating shaft; 13. Gear ring; 14. Triangular block; 15. Connecting shaft; 16. First air outlet plate; 17. Second air outlet plate; 18. Air outlet; 19. First crushing plate; 20. Second crushing plate; 21. First base plate; 22. Fixing plate; 23. Impeller; 24. Mounting block; 25. Crushing column; 26. Cleaning sleeve column. 27. Scraper; 28. Pushing block; 29. ​​First spring; 30. Cam; 31. Monitoring ring; 32. Upper top plate; 33. Lower bottom plate; 34. First sliding groove; 35. Connecting block; 36. Moving column; 37. Moving plate; 38. Second sliding groove; 39. Second spring; 40. Connecting column; 41. Feed pipe; 42. Cooling water inlet; 43. Cooling water outlet; 44. Discharge port; 45. Steam inlet; 46. Viewing window; 47. Sterile air inlet; 48. Exhaust port; 49. Variable speed bearing; 50. Fixing rod; 51. Air guide hole. Detailed Implementation

[0022] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0023] Please see Figures 1-11 This invention provides a microbial fermentation device with a sealing detection function, including a base 1, a monitoring box 2 fixedly connected to one side of the top of the base 1, multiple sets of support legs 3 fixedly connected to the other side of the top of the base 1, a fermentation tank 4 arranged in the middle of the multiple sets of support legs 3, a cooling jacket 5 fixedly connected to the outside of the fermentation tank 4, and a bearing sleeve 6 fixedly connected to the middle of the top of the fermentation tank 4. A drive motor 7 is fixedly connected to the top of the bearing sleeve 6. A first rotating shaft 8 is fixedly connected to the output end of the drive motor 7. An mounting sleeve 9 is movably sleeved on the outside of the first rotating shaft 8. A coaxial reversing assembly is provided inside the mounting sleeve 9. The inside of the fermentation tank 4 is fixedly connected with a first bottom plate 21, the inside of the first bottom plate 21 is provided with a crushing assembly, and the outside of the first rotating shaft 8 is provided with a plurality of stirring assemblies; The outside of the first rotating shaft 8 is fixedly connected with a mounting block 24, and the inside of the mounting block 24 is provided with a scraping assembly; The outside of the top of the fermentation tank 4 is movably sleeved with a monitoring ring 31, the inside of the monitoring ring 31 is provided with a monitoring assembly, and through the cooperation of the coaxial reversing assembly and the crushing assembly, the bubbles entering the inside of the fermentation tank 4 can be subdivided into countless small bubbles, so that the oxygen content in the liquid culture medium is improved, the uniform distribution of oxygen in the inside of the fermentation tank 4 is ensured, and the fermentation efficiency is improved.

[0024] The coaxial reversing assembly comprises a driving gear 10 fixedly sleeved on the outside of the first rotating shaft 8, a plurality of driven gears 11 meshingly connected on the outside of the driving gear 10, a second rotating shaft 12 fixedly connected in the plurality of driven gears 11, the plurality of second rotating shafts 12 rotatably connected with the mounting sleeve 9, a gear ring 13 fixedly connected in the mounting sleeve 9 and meshingly connected with the plurality of driven gears 11, a triangular block 14 movably sleeved on the outside of the plurality of second rotating shafts 12, a connecting shaft 15 fixedly connected to the middle of one side of the triangular block 14 and movably sleeved on the outside of the first rotating shaft 8, a fixed rod 50 fixedly connected to one side of the mounting sleeve 9 and fixedly connected with the fermentation tank 4, and the connecting shaft 15 rotatably connected with the mounting sleeve 9; Through the rotation of the first crushing plate 19 and the second crushing plate 20 for shearing the bubbles, the sterile air is further subdivided, the sterile air is better contacted with the liquid culture medium, the gas-liquid contact specific surface area can be significantly increased, the oxygen dissolution is accelerated, the rotation directions of the second air outlet plate 17 and the first air outlet plate 16 are opposite, a bidirectional stirring flow field is formed, the liquid laminar flow dead angle is eliminated, the uniform distribution of oxygen is ensured, the efficient dispersion of sterile air, the maximization of gas-liquid mass transfer and the optimization of mixing uniformity are ensured during work, the fermentation efficiency is improved, the energy consumption and cost are reduced, and the process stability is ensured.

[0025] The crushing assembly comprises a first air outlet plate 16 fixedly sleeved on the outer side of the connecting shaft 15, the lower end of the first air outlet plate 16 is provided with a second air outlet plate 17 fixedly sleeved on the outer side of the first rotating shaft 8, the middle part of the first air outlet plate 16 and the second air outlet plate 17 is provided with a plurality of groups of air outlet holes 18, the crushing assembly further comprises a plurality of groups of first crushing plates 19 fixedly connected to the lower surface of the inside of the first air outlet plate 16, the top of the second air outlet plate 17 is fixedly connected with a plurality of groups of second crushing plates 20, the teeth of the first crushing plate 19 and the second crushing plate 20 are staggered, the inside of the first bottom plate 21 is provided with a gas guide hole 51, one side of the bottom of the fermentation tank 4 is fixedly connected with a sterile air inlet 47, the sterile air inlet 47 is communicated with the gas guide hole 51, so as to conveniently convey the gas to the inside of the fermentation tank 4. The driving motor 7 is started to drive the first rotating shaft 8 to rotate, the first rotating shaft 8 drives the driving gear 10 to rotate, the driving gear 10 drives the driven gear 11 to rotate along the gear ring 13, the driven gear 11 drives the second rotating shaft 12 and the triangular block 14 to rotate, the triangular block 14 drives the connecting shaft 15 to rotate, the connecting shaft 15 rotates to make the first air outlet plate 16 rotate, at this time, the rotation of the first rotating shaft 8 drives the second air outlet plate 17 to rotate, due to the wheel system in the mounting sleeve 9, the rotation direction of the connecting shaft 15 and the first rotating shaft 8 is changed, so that the first air outlet plate 16 and the second air outlet plate 17 rotate in opposite directions, realizing coaxial reverse rotation, after the sterile air enters the inside of the first air outlet plate 16 and the second air outlet plate 17, the rotating first air outlet plate 16 and the second air outlet plate 17 drive the first crushing plate 19 and the second crushing plate 20 to crush them, so that they become a plurality of small bubbles, at the same time, due to the staggered arrangement of the first crushing plate 19 and the second crushing plate 20, the bubbles in the first air outlet plate 16 and the second air outlet plate 17 are sheared in the rotating process, so that the bubbles are further sheared to become smaller bubbles, thereby significantly increasing the gas-liquid contact specific surface area, accelerating oxygen dissolution, at the same time, due to the opposite rotation directions of the second air outlet plate 17 and the first air outlet plate 16, a bidirectional stirring flow field is formed, liquid laminar flow dead angle is eliminated, oxygen is uniformly distributed, high-efficiency dispersion of sterile air, maximization of gas-liquid mass transfer and optimization of mixing uniformity are ensured in work, fermentation efficiency is improved, energy consumption and cost are reduced, and process stability is ensured.

[0026] The stirring assembly comprises a fixed plate 22, a plurality of impellers 23 are fixedly connected to the outer side of the fixed plate 22. The rotation of the first rotating shaft 8 can also drive the fixed plate 22 and the impeller 23 to rotate, stirring the microbial fermentation, keeping the microbial fermentation in a suspended state, avoiding sediment accumulation, ensuring uniform distribution of the bacterial body and sufficient contact with the culture medium, thereby accelerating the metabolic reaction rate of the substrate and the microorganism, and promoting gas-liquid mass transfer at the same time. The broken bubbles increase the oxygen dissolution efficiency, especially for aerobic fermentation, and improve the uniformity of temperature, pH and nutrients, preventing local environmental differences from causing a decrease in fermentation efficiency. In addition, stirring can also reduce the risk of clumping in the liquid medium, maintain a fluidized state, promote the timely diffusion of metabolic products, reduce the probability of the growth of miscellaneous bacteria, and improve the overall fermentation stability and product yield.

[0027] The scraping assembly includes a breaking column 25 fixedly connected to the inside of the mounting block 24, a cleaning sleeve column 26 movably sleeved on the outside of the breaking column 25, a scraper 27 fixedly connected to the inside of the cleaning sleeve column 26, the inner circle of the scraper 27 abutting against the outer surface of the breaking column 25, a push block 28 fixedly connected to the top of the cleaning sleeve column 26, one side of the push block 28 fixedly connected to one end of a first spring 29, the other end of the first spring 29 fixedly connected to the mounting block 24, and a variable speed bearing 49 fixedly sleeved on the outside of the first rotating shaft 8, the outside of the variable speed bearing 49 fixedly connected with a cam 30, the cam 30 and the push block 28 arranged on the same axis, the height of the cam 30 and the push block 28 being equal; The rotation of the first rotating shaft 8 drives the mounting block 24 and the breaking column 25 to rotate, and the breaking column 25 rotates to mechanically defoam the foam generated during the microbial reaction. After the breaking column 25 contacts the foam multiple times, its surface is rich in water, which may reduce the defoaming efficiency when eliminating the foam. At the same time, the rotation speed of the cam 30 is slower than that of the mounting block 24 driven by the first rotating shaft 8 due to the arrangement of the variable speed bearing 49. At this time, the cam 30 contacts the push block 28 and pushes it out, and the push block 28 drives the cleaning sleeve column 26 and the scraper 27 to scrape off the hydration layer attached to the surface of the breaking column 25, reducing the interference of water in the foam film and enhancing the direct action of the defoaming device. At the same time, the hydration layer may cause corrosion or scaling on the surface of the breaking column 25, and the scraping action of the breaking column 25 can prolong the service life of the equipment and reduce maintenance costs. After the cam 30 rotates away, the first spring 29 drives the push block 28 to reset, realizing a cycle of work.

[0028] The monitoring assembly comprises an upper top plate 32 and a lower bottom plate 33, the upper top plate 32 and the lower bottom plate 33 are fixedly connected to the upper surface inside the monitoring ring 31 and the lower surface inside the monitoring ring 31 respectively, the inner side surface of the upper top plate 32 is provided with a plurality of first sliding grooves 34, the plurality of first sliding grooves 34 are slidably connected with connecting blocks 35, one side of the connecting block 35 is fixedly connected with a moving column 36, the outer side of the upper top plate 32 movably sleeves a moving plate 37, the other side of the connecting block 35 is fixedly connected with the moving plate 37, the monitoring assembly further comprises a plurality of second sliding grooves 38, the plurality of second sliding grooves 38 are provided in the inside of the lower bottom plate 33, one end of the second spring 39 is fixedly connected with the inside of the plurality of second sliding grooves 38, the other end of the second spring 39 is fixedly connected with a connecting column 40, the connecting column 40 is slidably connected with the second sliding groove 38, the connecting column 40 is fixedly connected with the moving plate 37, and the width of the lower bottom plate 33 is equal to the sum of the upper top plate 32 and the moving plate 37; When the monitoring ring 31 is sleeved on the connecting part of the fermentation tank 4, the edge of the fermentation tank 4 will lift the moving column 36, at this time the moving column 36 will lift the connecting block 35 and the moving plate 37, thereby opening the inside of the monitoring ring 31, the water flow in the monitoring ring 31 can seal the connecting part of the fermentation tank 4, and the connecting part can be monitored in real time during the working process, so as to avoid the risk of leakage and improve the safety of the system. The water flow pressure is self-adaptive to the deformation of the connecting part, and the sealing and monitoring functions are integrated.

[0029] The fermentation tank 4 is fixedly connected with a feeding pipe 41 on one side of the top, the cooling layer 5 is fixedly connected with a cooling water inlet 42 on one side, the cooling layer 5 is fixedly connected with a cooling water outlet 43 on the other side, the bottom of the fermentation tank 4 is fixedly connected with a discharge port 44, one side of the discharge port 44 is fixedly connected with a steam inlet 45, one side of the cooling layer 5 is fixedly connected with a window 46, the top of the fermentation tank 4 is fixedly connected with an exhaust port 48, and the cooling layer 5 is fixedly connected with the supporting leg 3. Through a plurality of pipelines, water cooling, gas outlet, feeding, discharging, water outlet and other work are realized, which provides effective help for microbial reaction.

[0030] Working principle: In the working process, the raw materials to be fermented are injected into the inside of the fermentation tank 4 through the feeding pipe 41, and sterile air is introduced into the inside of the fermentation tank 4 through the sterile air inlet 47 in the process of microbial reaction in the inside of the fermentation tank 4, so as to ensure the oxygen content in the inside of the fermentation tank 4. At this time, the first rotating shaft 8 can be driven to rotate by starting the driving motor 7, the first rotating shaft 8 drives the driving gear 10 to rotate, the driving gear 10 drives the driven gear 11 to rotate along the gear ring 13, the driven gear 11 drives the second rotating shaft 12 and the triangular block 14 to rotate, the triangular block 14 drives the connecting shaft 15 to rotate, and the first air outlet plate 16 will rotate after the connecting shaft 15 rotates. At this time, the rotation of the first rotating shaft 8 will drive the second air outlet plate 17 to rotate. At this time, the introduced sterile air will preferentially contact the second air outlet plate 17 through the air guide hole 51. At this time, the rotating second air outlet plate 17 will block the rising sterile air to increase the time of the air in the liquid culture medium, so as to make more oxygen dissolve into the liquid culture medium; The sterile air continues to rise to the middle of the first air outlet plate 16 and the second air outlet plate 17. At this time, due to the change of the rotation direction of the connecting shaft 15 and the first rotating shaft 8 caused by the wheel system in the installation sleeve 9, the first air outlet plate 16 and the second air outlet plate 17 rotate in opposite directions, realizing coaxial reverse rotation. After the sterile air enters the inside of the first air outlet plate 16 and the second air outlet plate 17, the rotating first air outlet plate 16 and the second air outlet plate 17 will drive the first crushing plate 19 and the second crushing plate 20 to crush them, so as to become a plurality of small bubbles. At the same time, due to the staggered arrangement of the first crushing plate 19 and the second crushing plate 20, the bubbles in the first air outlet plate 16 and the second air outlet plate 17 will be sheared in the process of rotation, so as to further cut the bubbles, making them become smaller bubbles, thereby significantly increasing the gas-liquid contact specific surface area, accelerating oxygen dissolution, and at the same time, the rotation direction of the second air outlet plate 17 and the first air outlet plate 16 is opposite, forming a two-way stirring flow field, eliminating the liquid laminar flow dead angle, and ensuring the uniform distribution of oxygen. In the working process, the efficient dispersion of sterile air, the maximization of gas-liquid mass transfer and the optimization of mixing uniformity can be ensured, the fermentation efficiency can be improved, the energy consumption and cost can be reduced, and the process stability can be ensured; At the same time, the rotation of the first rotating shaft 8 can also drive the fixed plate 22 and the impeller 23 to rotate, and stir the microbial fermentation, so that the microorganisms maintain a suspended state during the fermentation process, avoid sediment accumulation, ensure uniform distribution of bacteria and sufficient contact with the culture medium, thereby accelerating the metabolic reaction rate of the substrate and the microorganisms. Stirring can also promote gas-liquid mass transfer at the same time, increase oxygen dissolution efficiency through bubble crushing, especially for aerobic fermentation, and improve temperature, pH and uniformity of nutrients, prevent local environmental differences from causing fermentation efficiency to decrease. In addition, stirring can also reduce the risk of clumping in the liquid culture medium, maintain fluidization, promote the timely diffusion of metabolic products, reduce the probability of the growth of miscellaneous bacteria, and improve the overall fermentation stability and product yield. At the same time, the first rotating shaft 8 rotates to drive the mounting block 24 and the breaking column 25 to rotate, and the breaking column 25 rotates to mechanically defoam the foam generated in the microbial reaction process. After the breaking column 25 contacts the foam for multiple times, the surface of the breaking column 25 is rich in water, which may reduce the defoaming efficiency when the foam is eliminated by impact. At the same time, due to the setting of the variable speed bearing 49, the rotating speed of the cam 30 is slower than that of the mounting block 24 driven by the first rotating shaft 8. At this time, the cam 30 contacts the push block 28 to push the push block 28 out, and the push block 28 drives the cleaning sleeve column 26 and the scraper 27 to remove the hydration layer attached to the surface of the breaking column 25, which can reduce the water interference in the foam film, enhance the direct action of the defoaming device, avoid the defoaming efficiency reduction caused by the water layer blocking, and also avoid the corrosion or scaling of the surface of the breaking column 25 caused by the hydration layer. Through the scraping action of the breaking column 25, the service life of the equipment can be prolonged, and the maintenance cost can be reduced. After the cam 30 rotates away, the first spring 29 drives the push block 28 to reset, realizing the cycle work. When the monitoring ring 31 is sleeved on the connection of the fermentation tank 4, the edge of the fermentation tank 4 will lift the moving column 36, and the moving column 36 will lift the connecting block 35 and the moving plate 37 to open the inside of the monitoring ring 31, so that the water flow in the monitoring ring 31 liquid seals the connection of the fermentation tank 4. During the work process, the connection can be monitored in real time through the monitoring ring 31 to avoid the risk of leakage and improve the system safety. The water flow pressure self-adapts to the deformation of the connection, and integrates the sealing and monitoring functions.

[0031] After the internal microbial reaction is completed, the product is taken out through the discharge port 44, the discharge is completed, and the work is ended.

[0032] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not limited. Although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application, which should be covered in the scope of the claims of the present application.

Claims

1. A microorganism fermentation device with a sealing detection function, comprising a base (1), characterized in that, The top of the base (1) is fixedly connected with a monitoring box (2), the other side of the top of the base (1) is fixedly connected with a plurality of groups of supporting legs (3), the middle of the plurality of groups of supporting legs (3) is provided with a fermentation tank (4), the outer side of the fermentation tank (4) is fixedly connected with a cooling sandwich (5), the middle of the top of the fermentation tank (4) is fixedly connected with a bearing sleeve (6); The top of the bearing sleeve (6) is fixedly connected with a driving motor (7), the output end of the driving motor (7) is fixedly connected with a first rotating shaft (8), the outer side of the first rotating shaft (8) movably sleeved with a mounting sleeve (9), the inside of the mounting sleeve (9) is provided with a coaxial reverse component; The inside of the first bottom plate (21) is provided with a crushing component, the outer side of the first rotating shaft (8) is provided with a plurality of groups of stirring components; The outer side of the first rotating shaft (8) is fixedly connected with a mounting block (24), the inside of the mounting block (24) is provided with a scraping component; The outer side of the top of the fermentation tank (4) movably sleeved with a monitoring ring (31), the inside of the monitoring ring (31) is provided with a monitoring component.

2. The microorganism fermentation device with a sealing detection function according to claim 1, characterized in that, The coaxial reverse component includes a driving gear (10), the driving gear (10) is fixedly sleeved on the outer side of the first rotating shaft (8), the outer side of the driving gear (10) is engagedly connected with a plurality of groups of driven gears (11), the inside of the plurality of groups of driven gears (11) is fixedly connected with a second rotating shaft (12), the plurality of groups of second rotating shafts (12) are rotatably connected with the mounting sleeve (9), the inside of the mounting sleeve (9) is fixedly connected with a gear ring (13), the gear ring (13) is engagedly connected with the plurality of groups of driven gears (11), the outer side of the plurality of groups of second rotating shafts (12) movably sleeved with a triangular block (14), the middle of one side of the triangular block (14) is fixedly connected with a connecting shaft (15), the connecting shaft (15) movably sleeved on the outer side of the first rotating shaft (8), one side of the mounting sleeve (9) is fixedly connected with a fixed rod (50), the fixed rod (50) is fixedly connected with the fermentation tank (4), the connecting shaft (15) is rotatably connected with the mounting sleeve (9).

3. The microorganism fermentation device with a sealing detection function according to claim 2, characterized in that, The crushing component includes a first air outlet plate (16), the first air outlet plate (16) is fixedly sleeved on the outer side of the connecting shaft (15), the lower end of the first air outlet plate (16) is provided with a second air outlet plate (17), the second air outlet plate (17) is fixedly sleeved on the outer side of the first rotating shaft (8), the middle of the first air outlet plate (16) and the second air outlet plate (17) is provided with a plurality of groups of air outlet holes (18).

4. The microorganism fermentation device with a sealing detection function according to claim 3, characterized in that, The smashing assembly further comprises a plurality of first smashing plates (19), each of which is fixedly connected to the lower surface inside the first air outlet plate (16); the top of the second air outlet plate (17) is fixedly connected with a plurality of second smashing plates (20); the teeth of the first smashing plates (19) and the second smashing plates (20) are staggered; the inside of the first bottom plate (21) is provided with an air guide hole (51); one side of the bottom of the fermentation tank (4) is fixedly connected with a sterile air inlet (47); the sterile air inlet (47) is in communication with the air guide hole (51), so as to facilitate the delivery of gas to the inside of the fermentation tank (4).

5. The microorganism fermentation device with a sealing detection function according to claim 4, characterized in that, The stirring assembly comprises a fixed plate (22), and a plurality of impellers (23) are fixedly connected to the outer side of the fixed plate (22).

6. The microorganism fermentation device with a sealing detection function according to claim 5, characterized in that, The scraping assembly comprises a breaking column (25) fixedly connected to the inside of the mounting block (24) on both sides; a cleaning sleeve column (26) movably sleeved on the outer side of the breaking column (25); a scraper (27) fixedly connected to the inside of the cleaning sleeve column (26); the inner ring of the scraper (27) abuts against the outer surface of the breaking column (25); a pushing block (28) fixedly connected to the top of the cleaning sleeve column (26); one side of the pushing block (28) is fixedly connected with one end of a first spring (29); the other end of the first spring (29) is fixedly connected with the mounting block (24).

7. The microorganism fermentation device with a sealing detection function according to claim 6, characterized in that, The scraping assembly further comprises a variable speed bearing (49) fixedly sleeved on the outer side of the first rotating shaft (8); a cam (30) fixedly connected to the outer side of the variable speed bearing (49); the cam (30) and the pushing block (28) are arranged on the same axis; the height of the cam (30) is equal to that of the pushing block (28).

8. The microorganism fermentation device with a sealing detection function according to claim 7, characterized in that, The monitoring assembly comprises an upper top plate (32) and a lower bottom plate (33), which are respectively fixedly connected to the upper surface inside the monitoring ring (31) and the lower surface inside the monitoring ring (31); a plurality of first sliding grooves (34) are formed in the inner side surface of the upper top plate (32); a connecting block (35) is slidably connected to the inside of each of the first sliding grooves (34); one side of the connecting block (35) is fixedly connected with a moving column (36); a moving plate (37) movably sleeved on the outer side of the upper top plate (32); the other side of the connecting block (35) is fixedly connected with the moving plate (37).

9. The microorganism fermentation device having a sealing detection function according to claim 8, characterized in that, The monitoring assembly further comprises a plurality of second sliding grooves (38), each of which is formed in the inside of the lower bottom plate (33); one end of a second spring (39) is fixedly connected to the inside of each of the second sliding grooves (38); the other end of the second spring (39) is fixedly connected with a connecting column (40); the connecting column (40) is slidably connected with the second sliding groove (38); the connecting column (40) is fixedly connected with the moving plate (37); the width of the lower bottom plate (33) is equal to the sum of the upper top plate (32) and the moving plate (37).

10. The microorganism fermentation device having a sealing detection function according to claim 9, wherein One side of the top of the fermenter (4) is fixedly connected with a feeding pipe (41), one side of the cooling sandwich (5) is fixedly connected with a cooling water inlet (42), the other side of the cooling sandwich (5) is fixedly connected with a cooling water outlet (43), the bottom of the fermenter (4) is fixedly connected with a discharge port (44), one side of the discharge port (44) is fixedly connected with a steam inlet (45), one side of the cooling sandwich (5) is fixedly connected with a window (46), the top of the fermenter (4) is fixedly connected with an exhaust port (48), and the cooling sandwich (5) is fixedly connected with the supporting leg (3).

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