Pet nucleic acid isothermal detection equipment with constant temperature regulation control function
By using an expansion heat dissipation component and fan design in the pet nucleic acid testing device, the problem of the buffer structure affecting heat exchange was solved, achieving rapid cooling and stability, and improving the nucleic acid testing efficiency and safety of the device.
Patent Information
- Application Number
- CN202511037886.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-28
- Publication Date
- 2025-11-18
AI Technical Summary
Existing pet nucleic acid isothermal testing equipment suffers from reduced testing efficiency in high-temperature outdoor environments due to the buffer structure affecting heat exchange, leading to prolonged cooling time of test tubes, nucleic acid sample amplification failure, or increased amplification time.
The device employs an expansion heat dissipation component and a fan design. When the temperature is triggered, the expansion bar expands the elastic wrapping layer to form a heat dissipation channel. The fan and the chiller work together to increase the heat exchange area between the test tube and the outside environment. The movable base plate design maintains the stability of the heat dissipation channel and achieves rapid cooling.
This improves the efficiency of nucleic acid testing in high-temperature environments, ensures that test tubes quickly return to the optimal reaction temperature, avoids sample leakage, and guarantees the safety and stability of the equipment.
Smart Images
Figure CN120966618A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the field of outdoor pet nucleic acid detection technology, in particular, to a pet nucleic acid isothermal detection equipment with constant temperature regulation control. BACKGROUND
[0002] The pet nucleic acid isothermal detection equipment is a detection instrument based on constant temperature nucleic acid amplification technology, which is designed for rapid nucleic acid screening of pet pathogens (viruses, bacteria, parasites, etc.), and can complete nucleic acid amplification and detection at a constant temperature, greatly simplifying the operation process. In actual use, the equipment often needs to perform nucleic acid detection in an outdoor environment, so the device usually has a device for maintaining the reaction chamber at a constant temperature.
[0003] The existing equipment is often carried by the staff for outdoor work, so the reaction chamber is usually wrapped with a buffer such as rubber to fix the test tube containing the detection sample, so as to avoid sample leakage caused by test tube shaking. However, this buffer structure will affect the heat preservation effect of the constant temperature maintaining device in actual application: when working outdoors in high temperature weather, the temperature inside the device will exceed the optimal temperature (60-65℃) for nucleic acid amplification due to the influence of external temperature, sunlight and heat generated by the motor and other devices inside the device, at which time the constant temperature maintaining device will dissipate heat to the reaction chamber; the test tube is wrapped outside by the buffer, which is difficult to exchange heat with the outside, so that the time required for the test tube and the sample inside to cool down is prolonged, and the nucleic acid sample will fail to amplify or the time required for amplification will increase, which will further cause the nucleic acid amplification and detection to be difficult to be quickly performed, and seriously reduce the detection efficiency of the equipment. SUMMARY
[0004] The present application provides a pet nucleic acid isothermal detection equipment with constant temperature regulation control, which sets an expansion heat dissipation assembly, uses the automatic expansion of the expansion strip when the trigger temperature is reached to expand the mounting groove of the elastic wrapping layer, separates the movable bottom plate from the bottom of the reaction chamber, forms first and second heat dissipation channels to increase the heat exchange area of the test tube, improves the cooling effect of the equipment on the test tube, uses the fan, branch pipe and two air outlet cylinders to quickly blow out hot air from the machine box, and cooperates with the movable bottom plate provided with a lower convex surface to make the airflow generate a downward thrust on the movable bottom plate to maintain the stability of the second heat dissipation channel, thereby solving the problems in the background art, that is:
[0005] The buffer structure of the conventional equipment will wrap the test tube, reduce the heat exchange area of the test tube, and thus cause the test tube to take a long time to cool down, the nucleic acid sample will fail to amplify or the time required for amplification will increase, and the nucleic acid detection efficiency of the equipment will be reduced.
[0006] In order to achieve the above object, the pet nucleic acid isothermal detection equipment with constant temperature regulation control comprises a cabinet, a reaction bin is fixedly arranged in the cabinet, a heating and heat preservation assembly is arranged outside the reaction bin, the heating and heat preservation assembly is used for improving the internal temperature of the reaction bin and heat preservation of the reaction bin, a plurality of elastic wrapping layers are fixedly arranged in the reaction bin, and the elastic wrapping layers are used for wrapping the fixed test tube.
[0007] The inside of the elastic wrapping layer is provided with a plurality of expansion heat dissipation assemblies, the bottom of the reaction bin is hollowed out and is provided with a movable bottom plate, the expansion heat dissipation assemblies are used for expanding the elastic wrapping layer when the temperature of the reaction bin exceeds the optimum temperature of nucleic acid amplification, so that the first heat dissipation channel is formed between the elastic wrapping layer and the test tube, and the movable bottom plate is pushed to be separated from the bottom of the reaction bin, so that the second heat dissipation channel is formed.
[0008] The inside of the cabinet is also provided with a fan and a refrigerator, and the air outlet direction of the fan is towards the refrigerator.
[0009] In the above technical scheme, when the temperature of the reaction bin exceeds the optimum temperature of nucleic acid amplification, the expansion heat dissipation assemblies expand the elastic wrapping layer and push the movable bottom plate downward, so that the first and second heat dissipation channels are formed between the elastic wrapping layer and the test tube and between the bottom of the reaction bin and the movable bottom plate respectively, the heat exchange area of the test tube with the outside is increased, the cooling efficiency of the equipment on the test tube is improved, the temperature in the test tube can be quickly recovered to the optimum reaction temperature of nucleic acid amplification by cooperating with the fan and the refrigerator, and the detection efficiency of the equipment in the high-temperature environment is improved.
[0010] On this basis, the material of the elastic wrapping layer is rubber, the top surface of the elastic wrapping layer is provided with an installation groove for wrapping and installing the test tube, a plurality of branch grooves are arranged on the inner wall of the installation groove, and the expansion heat dissipation assemblies are arranged in the branch grooves.
[0011] The expansion heat dissipation assembly comprises an expansion strip fixedly arranged in the branch groove, the material of the expansion strip is memory alloy, the trigger temperature of expansion of the expansion strip is set to ℃, a rubber pad is fixedly connected to one side of the expansion strip close to the test tube, and the rubber pad is attached to the test tube.
[0012] The overall shape of the expansion strip is L-shaped, the bottom surface of the expansion strip is fixedly connected to the top surface of the movable bottom plate through the rubber pad, reset spring rods are fixedly connected to the bottom surface of the movable bottom plate at four corners, and the bottom of the reset spring rod is fixedly connected to the inside of the cabinet.
[0013] In the technical scheme, the trigger temperature of the expansion strip is set to be the same as the maximum of the optimal temperature of the nucleic acid amplification reaction, when the temperature of the reaction chamber exceeds the maximum of the optimal temperature, the expansion strip expands automatically to expand the mounting groove, and the movable bottom plate is separated from the bottom of the reaction chamber to form the first and second heat dissipation channels, the test tube is clamped and fixed by the rubber pad on the side of the expansion strip to avoid the test tube from shaking to cause the nucleic acid sample to leak during heat dissipation, and the safety of the equipment is ensured.
[0014] In another technical scheme, the air outlet of the fan is communicated with a branch pipe, and the branch pipe comprises at least two air outlet ends, and each air outlet end is fixedly provided with an air outlet cylinder.
[0015] One of the air outlet cylinders is arranged above the reaction chamber, and the other air outlet cylinder is arranged at the connection between the reaction chamber and the movable bottom plate.
[0016] The bottom surface of the movable bottom plate is a lower convex surface, when the other air outlet cylinder blows air, part of the air passes through the lower convex surface, and the lower convex surface forms a low pressure area below the movable bottom plate, so that the overall air flow generates a downward thrust on the movable bottom plate, the upward reaction force of the reset spring rod on the movable bottom plate is dispersed, and the stability of the second heat dissipation channel is maintained.
[0017] In the technical scheme, the air of the fan is guided to the upper and lower sides of the reaction chamber through the branch pipe and the air outlet cylinder, the air outlet cylinder above blows the hot air gathered above the reaction chamber out of the machine box to achieve rapid heat dissipation, the air outlet cylinder below blows air to the first and second heat dissipation channels to dissipate heat, and the lower convex surface of the movable bottom plate can also cooperate with the air outlet cylinder below to form a low pressure area below the movable bottom plate, so that the air flow generates a downward pressure on the movable bottom plate, thereby dispersing the upward reaction force of the reset spring rod on the movable bottom plate, maintaining the stability of the second heat dissipation channel formed between the movable bottom plate and the reaction chamber, and ensuring the heat dissipation effect of the equipment on the test tube.
[0018] Compared with the prior art, the beneficial effects of the present application are:
[0019] In the pet nucleic acid isothermal detection equipment with constant temperature regulation control, the expansion heat dissipation assembly is arranged, the trigger temperature of the expansion strip in the expansion heat dissipation assembly is set to be the same as the maximum of the optimal temperature of the nucleic acid amplification reaction, when the temperature of the reaction chamber exceeds the maximum of the optimal temperature, the expansion strip expands automatically to expand the mounting groove and push the movable bottom plate away from the reaction chamber to form the first and second heat dissipation channels, the area of heat exchange between the test tube and the outside is increased, the heat dissipation effect of the fan and the refrigerator on the test tube and the elastic wrapping layer is improved, rapid cooling is achieved, and the expansion of the expansion strip and the cooperation of the rubber pad can also produce stable clamping action on the test tube during heat dissipation, so that the shaking of the test tube during heat dissipation is avoided to cause the leakage of the pathogen sample, and the safety of the equipment is ensured.
[0020] Meanwhile, the fan's airflow is guided to the upper and lower sides of the reaction chamber through branch pipes and air outlets. The upper air outlet blows the hot air gathered above the reaction chamber out of the casing, achieving rapid heat dissipation. The lower air outlet, while blowing air to dissipate heat from the first and second heat dissipation channels, also works with the convex surface of the movable base plate to create a low-pressure area below the movable base plate. This causes the airflow to exert downward pressure on the movable base plate, thereby dispersing the upward reaction force of the reset spring rod on the movable base plate and maintaining the stability of the second heat dissipation channel formed between the movable base plate and the reaction chamber, ensuring the equipment's heat dissipation effect on the test tubes. Attached Figure Description
[0021] Figure 1 This is a schematic diagram of the overall structure of the present invention;
[0022] Figure 2 This is a schematic diagram of the internal structure of the chassis of the present invention;
[0023] Figure 3 This is a schematic diagram of the overall structure of some parts of the present invention;
[0024] Figure 4 This is a schematic diagram of the overall structure of the reaction chamber after it has been separated from the heating and insulation components and the movable base plate in this invention;
[0025] Figure 5 This is a schematic diagram of the internal structure of the insulation layer in this invention;
[0026] Figure 6 This is a cross-sectional view of the elastic wrapping layer and a disassembled structural diagram of the expansion heat dissipation component in this invention.
[0027] Figure 7 For the present invention Figure 6 Enlarged schematic diagram of the structure at point A;
[0028] Figure 8 This is a schematic diagram showing the state of the mounting groove before and after it is expanded by the expansion strip in this invention;
[0029] Figure 9 This is a partial structural diagram of the movable base plate after it is separated from the reaction chamber in this invention.
[0030] The meanings of the labels in the diagram are as follows:
[0031] 1. Chassis;
[0032] 2. Reaction chamber;
[0033] 3. Heating and insulation components; 31. Insulation layer; 32. Heating element; 33. Temperature sensor;
[0034] 4. Elastic wrapping layer; 41. Mounting groove; 42. Branch groove;
[0035] 5, inflation heat dissipation assembly; 51, inflation strip; 52, rubber pad;
[0036] 6, movable bottom plate; 61, lower convex surface;
[0037] 7, reset spring rod;
[0038] 8, fan; 81, branch pipe; 82, air outlet cylinder;
[0039] 9, refrigeration machine. DETAILED DESCRIPTION
[0040] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0041] Currently, the buffer structure of the test tube in the traditional device will wrap the test tube, reduce the heat exchange area of the test tube, and thus cause the test tube to have a long cooling time, and the nucleic acid sample will thus have the phenomenon of amplification failure or increased amplification time, thereby reducing the nucleic acid detection efficiency of the device. The present application provides a pet nucleic acid isothermal detection device with constant temperature regulation control, as shown in Figure 1 Figure 3 The inside of the case 1 is provided with a reaction bin 2, the outside of the reaction bin 2 is provided with a heating and heat preservation assembly 3, the heating and heat preservation assembly 3 is used to improve the temperature inside the reaction bin 2 and heat preservation of the reaction bin 2, the inside of the reaction bin 2 is provided with a plurality of elastic wrapping layers 4, the elastic wrapping layer 4 is used to wrap the test tube, the inside of the elastic wrapping layer 4 is further provided with a plurality of inflation heat dissipation assemblies 5, the inflation heat dissipation assembly 5 will expand the elastic wrapping layer 4 when the temperature in the reaction bin 2 exceeds the optimum temperature of nucleic acid amplification, so as to form a first heat dissipation channel between the elastic wrapping layer 4 and the test tube, and at the same time fix the test tube, the inside of the case 1 is further provided with a fan 8 and a refrigeration machine 9, the fan 8 accelerates the heat in the case 1 to be dissipated outward by blowing, and cooperates with the refrigeration machine 9 to make the internal temperature of the case 1 and the reaction bin 2 rapidly decrease in a high temperature environment, the air outlet direction of the fan 8 is towards the refrigeration machine 9, and the fan 8 can send the heat of the refrigeration machine 9 generated by heat exchange out of the case 1 when blowing.
[0042] Specifically, as shown in Figure 4 、 Figure 6 and Figure 7 The elastic wrapping layer 4 is made of rubber, and the top surface of the elastic wrapping layer 4 is provided with a mounting groove 41 for mounting the test tube, and the inner wall of the mounting groove 41 is provided with a plurality of branch grooves 42 distributed uniformly, and the expansion heat dissipation assembly 5 is arranged in the branch grooves 42.
[0043] The expansion heat dissipation assembly 5 comprises an expansion strip 51 fixedly arranged in the branch grooves 42, the expansion strip 51 is made of memory alloy, and the side of the expansion strip 51 close to the test tube is fixedly connected with a rubber pad 52, and the rubber pad 52 is attached to the test tube.
[0044] Cooperation Figure 8 In the embodiment, the rubber itself is a material with heat preservation and shock resistance effects, so the elastic wrapping layer 4 has heat preservation and shock resistance effects; the memory alloy will expand when the temperature of the outside environment exceeds the trigger temperature of the memory alloy, and the trigger temperature of the expansion strip 51 can be controlled by adjusting the proportion of the metal in the memory alloy, which is a prior art and will not be described in detail here. In actual use, the trigger temperature of the expansion strip 51 can be set to the maximum value (65℃) of the optimal temperature of nucleic acid amplification.
[0045] When the temperature inside the reaction chamber 2 exceeds the optimal reaction temperature (65℃) of nucleic acid amplification, the expansion strip 51 expands to expand and enlarge the mounting groove 41, so that a gap is formed between the mounting groove 41 and the test tube as a first heat dissipation channel of the test tube; at the same time, due to the expansion of the expansion strip 51, the rubber pad 52 is pressed by the expansion strip 51 outside the test tube, so that after the mounting groove 41 is enlarged, the rubber pad 52 and the expansion strip 51 form a new fixed clamping effect on the test tube, effectively avoiding the leakage of nucleic acid samples caused by the shaking of the test tube during heat dissipation.
[0046] By setting the expansion heat dissipation assembly 5 and setting the trigger temperature of the expansion of the expansion strip 51 to be the same as the optimal reaction temperature of nucleic acid amplification (65℃), when the temperature inside the reaction chamber 2 exceeds the optimal reaction temperature of nucleic acid amplification, the expansion strip 51 automatically expands to expand the mounting groove 41, so that a gap is formed between the mounting groove 41 and the test tube as a first heat dissipation channel of the test tube, which increases the contact area of the test tube with the outside environment and accelerates the heat exchange effect between the test tube and the outside environment, thereby improving the heat dissipation efficiency of the test tube. In a high temperature environment, the fan 8 and the refrigerator 9 can quickly restore the temperature in the test tube to the optimal reaction temperature of nucleic acid amplification, thereby improving the detection efficiency of the equipment in a high temperature environment.
[0047] At the same time, by using the expansion of the expansion strip 51, after the mounting groove 41 is enlarged, the expansion strip 51 and the rubber pad 52 replace the elastic wrapping layer 4 to clamp and fix the test tube, effectively avoiding the leakage of nucleic acid samples caused by the shaking of the test tube during heat dissipation, and improving the safety of the equipment.
[0048] In addition, the bottom of the reaction bin 2 is hollowed out, and the bottom of the reaction bin 2 is provided with a movable bottom plate 6, which is attached to the bottom of the reaction bin 2 when the temperature in the reaction bin 2 does not exceed the optimum temperature of nucleic acid amplification, and is separated from the bottom of the reaction bin 2 when the temperature in the reaction bin 2 exceeds the optimum temperature of nucleic acid amplification, so as to expose the mounting groove 41 and the bottom of the test tube;
[0049] The overall shape of the expansion strip 51 is L-shaped, and the bottom surface of the expansion strip 51 is fixedly connected to the top surface of the movable bottom plate 6 through the rubber pad 52;
[0050] In this embodiment, when the expansion strip 51 expands, its bottom pushes the movable bottom plate 6 downward by its own expansion, so that the movable bottom plate 6 is separated from the reaction bin 2, thereby exposing the mounting groove 41 and the bottom of the test tube, forming a second heat dissipation channel for overall heat dissipation of the reaction bin 2 and the test tube, and further improving the heat dissipation effect of the reaction bin 2 and the test tube.
[0051] Further, referring to Figure 5 As shown in the figure, the heating and heat preservation assembly 3 includes a heat preservation layer 31 wrapped outside the reaction bin 2, the inside of the heat preservation layer 31 is provided with a heating pipe 32, and the inside of the heat preservation layer 31 is provided with a temperature sensor 33;
[0052] The inside of the case 1 is provided with a temperature control system, the heating pipe 32, the temperature sensor 33, the fan 8 and the refrigerating machine 9 are electrically connected with the temperature control system; the temperature control system adopts a PID temperature control algorithm, combines with the real-time monitoring feedback of the temperature sensor 33 on the temperature of the reaction bin 2, can control the working state of the heating pipe 32, the fan 8 and the refrigerating machine 9 according to the actual temperature, and improves the efficiency of temperature regulation;
[0053] The bottom surface of the movable bottom plate 6 is fixedly connected with a reset spring rod 7 at four corners, and the bottom of the reset spring rod 7 is fixedly connected with the inside of the case 1;
[0054] In this embodiment, when the movable bottom plate 6 is separated from the reaction bin 2, the movable bottom plate 6 extrudes and shrinks the reset spring rod 7, after heat dissipation, the expansion strip 51 shrinks to the original size, the reset spring rod 7 releases the elastic force to push the movable bottom plate 6 back to the original position, and the movable bottom plate 6 re-closes the bottom surface of the reaction bin 2, and re-forms a complete heat preservation structure of the reaction bin 2 with the heat preservation layer 31.
[0055] Further, referring to Figure 8 As shown in the figure, the outlet of the fan 8 is communicated with a branch pipe 81, the branch pipe 81 includes at least two air outlets, and the two air outlets of the branch pipe 81 are fixedly provided with air outlet cylinders 82, one of which is arranged above the reaction bin 2, and the other is arranged at the connection between the reaction bin 2 and the movable bottom plate 6, and the bottom surface of the movable bottom plate 6 is provided as a lower convex surface 61;
[0056] In this embodiment, the fan 8 can blow air into the inside of the cabinet 1 through the branch pipe 81 and the air outlet 82 to dissipate heat. Since hot air generally moves upward, the air outlet 82 located above the reaction chamber 2 can quickly blow the hot air gathered above the reaction chamber 2 out of the inside of the cabinet 1.
[0057] The air outlet 82 located below the reaction chamber 2 can blow air to the bottom of the reaction chamber 2 after the movable bottom plate 6 is separated from the reaction chamber 2 to dissipate heat, thereby accelerating the heat dissipation of the exposed mounting groove 41 and the test tube. After the movable bottom plate 6 is separated from the reaction chamber 2, a second heat dissipation channel is formed between the two, and at this time, part of the air blown by the air outlet 82 at this position flows from the second heat dissipation channel to accelerate the heat dissipation in the first and second heat dissipation channels, and the other part flows from below the movable bottom plate 6. The lower convex surface 61 makes the overall shape of the movable bottom plate 6 similar to that of an airplane wing. According to Bernoulli's principle, the pressure above the movable bottom plate 6 is high and the pressure below the movable bottom plate 6 is low due to the different air flow rates on the upper and lower sides of the movable bottom plate 6, so that the air blown by the air outlet 82 forms a downward thrust on the movable bottom plate 6, which helps to disperse the upward reaction force of the reset spring rod 7 on the movable bottom plate 6, thereby maintaining the stability of the second heat dissipation channel formed between the movable bottom plate 6 and the reaction chamber 2.
[0058] The working principle of the device is as follows:
[0059] When the temperature of the reaction chamber 2 is lower than the optimal temperature (60℃) for nucleic acid amplification, the temperature sensor 33 first detects that the temperature of the reaction chamber 2 is low and sends an electrical signal to the temperature control system in the cabinet 1. After receiving the electrical signal, the temperature control system controls the heating tube 32 to turn on. After the heating tube 32 is started, the reaction chamber 2 is heated. When the temperature sensor 33 detects that the temperature of the reaction chamber 2 reaches the optimal temperature (60℃-65℃), it sends an electrical signal to the temperature control system again. After receiving the electrical signal, the temperature control system turns off the heating tube 32. At this time, the heat preservation layer 31 preserves the temperature of the reaction chamber 2.
[0060] When the temperature of the reaction chamber 2 is higher than the optimum temperature (65℃) of nucleic acid amplification, the temperature sensor 33 sends an electric signal to the temperature control system, and the temperature control system controls the fan 8 and the refrigerator 9 to start working under the cooperation of the two, so as to accelerate the heat dissipation of the inside of the machine box 1 and the reaction chamber 2; during the process, when the temperature of the reaction chamber 2 exceeds the optimum temperature, the expansion strip 51 reaches the trigger temperature and starts to expand, so as to expand the installation groove 41, separate the elastic wrapping layer 4 from the test tube, and form the first heat dissipation channel between the installation groove 41 and the test tube; at the same time, the bottom of the expansion strip 51 pushes the movable bottom plate 6 to move downwards after expansion, so as to separate the movable bottom plate 6 from the bottom of the reaction chamber 2, expose the bottom of the installation groove 41 and the test tube, and form the second heat dissipation channel; during the heat dissipation, the fan 8 blows air flow upwards and downwards of the reaction chamber 2 through the branch pipe 81 and the two air outlets 82, the air outlet 82 upwards blows the hot air gathered above the reaction chamber 2, so as to quickly dissipate the heat to the outside of the machine box 1, the air flow blown by the air outlet 82 downwards is separated by the movable bottom plate 6, a part of the air flow flows between the movable bottom plate 6 and the reaction chamber 2, so as to accelerate the heat dissipation in the first and second heat dissipation channels, and the other part of the air flow flows through the lower convex surface 61 of the bottom of the movable bottom plate 6, so as to generate a downward thrust on the movable bottom plate 6 through the Bernoulli principle, and disperse the upward reaction force of the reset spring rod 7 on the movable bottom plate 6, so as to maintain the stability of the second heat dissipation channel;
[0061] When the temperature of the reaction chamber 2 decreases to the optimum temperature range (greater than 60℃ and less than 65℃), the expansion strip 51 is no longer at the trigger temperature, the expansion strip 51 shrinks to rewrap the test tube with the installation groove 41, and the elastic force of the reset spring rod 7 makes the movable bottom plate 6 re-connect with the bottom of the reaction chamber 2, so as to close the first and second heat dissipation channels, and the movable bottom plate 6 and the heat preservation layer 31 re-combine to form a complete heat preservation structure for the reaction chamber 2;
[0062] Through the temperature monitoring of the temperature sensor 33 and the temperature control system adopting the PID temperature control algorithm, the heating pipe 32, the fan 8 and the refrigerator 9 work alternately, so as to maintain the constant temperature of the reaction chamber 2.
[0063] The above shows and describes the basic principles, main features and advantages of the present application. It should be understood by those skilled in the art that the present application is not limited to the above-mentioned embodiments, the above-mentioned embodiments and descriptions in the specification are only preferred examples of the present application, and are not intended to limit the present application, various changes and improvements can be made to the present application without departing from the spirit and scope of the present application, and these changes and improvements all fall within the scope of the present application. The scope of protection of the present application is defined by the appended claims and their equivalents.
Claims
1. A pet nucleic acid isothermal detection device with constant temperature regulation and control, comprising a chassis (1), wherein a reaction chamber (2) is fixedly installed inside the chassis (1), characterized in that: The reaction chamber (2) is provided with a heating and heat preservation component (3) on the outside. The heating and heat preservation component (3) is used to increase the internal temperature of the reaction chamber (2) and to keep the reaction chamber (2) warm. Several elastic wrapping layers (4) are fixedly provided inside the reaction chamber (2). The elastic wrapping layers (4) are used to wrap and fix the test tube. The elastic wrapping layer (4) is provided with several expansion heat dissipation components (5). The bottom of the reaction chamber (2) is hollowed out and is provided with a movable base plate (6). When the temperature of the reaction chamber (2) exceeds the optimal temperature for nucleic acid amplification, the expansion heat dissipation components (5) expand the elastic wrapping layer (4) to form a first heat dissipation channel between the elastic wrapping layer (4) and the test tube, and push the movable base plate (6) to separate it from the bottom of the reaction chamber (2) to form a second heat dissipation channel. The chassis (1) is also equipped with a fan (8) and a refrigeration unit (9), with the fan (8) having its air outlet direction facing the refrigeration unit (9).
2. The pet nucleic acid isothermal detection device with constant temperature regulation and control according to claim 1, characterized in that: The elastic wrapping layer (4) is made of rubber. The top surface of the elastic wrapping layer (4) is provided with an installation groove (41) for wrapping and installing test tubes. The inner wall of the installation groove (41) is provided with several evenly distributed branch grooves (42). The expansion heat dissipation component (5) is located inside the branch grooves (42).
3. The pet nucleic acid isothermal detection device with constant temperature regulation and control according to claim 2, characterized in that: The expansion heat dissipation component (5) includes an expansion strip (51) fixedly installed inside the branch groove (42). The material of the expansion strip (51) is a shape memory alloy. The trigger temperature for the expansion strip (51) to expand is set to 65°C. A rubber pad (52) is fixedly connected to the side of the expansion strip (51) near the test tube. The rubber pad (52) is in contact with the test tube.
4. The pet nucleic acid isothermal detection device with constant temperature regulation and control according to claim 3, characterized in that: The expansion bar (51) is L-shaped. The bottom surface of the expansion bar (51) is fixedly connected to the top surface of the movable base plate (6) through a rubber pad (52). A reset spring rod (7) is fixedly connected to each of the four corners of the bottom surface of the movable base plate (6). The bottom of the reset spring rod (7) is fixedly connected to the inside of the chassis (1).
5. The pet nucleic acid isothermal detection device with constant temperature regulation and control according to claim 1, characterized in that: The air outlet of the fan (8) is connected to a branch pipe (81), the branch pipe (81) includes at least two air outlets, and each of the two air outlets of the branch pipe (81) is fixedly provided with an air outlet duct (82).
6. The pet nucleic acid isothermal detection device with constant temperature regulation and control according to claim 5, characterized in that: One of the air outlets (82) is located above the reaction chamber (2), and the other air outlet (82) is located at the connection between the reaction chamber (2) and the movable base plate (6).
7. The pet nucleic acid isothermal detection device with constant temperature regulation and control according to claim 6, characterized in that: The bottom surface of the movable base plate (6) is a convex surface (61). When the other air outlet (82) blows out airflow, part of the airflow passes through the convex surface (61). Under the convex arc of the convex surface (61), a low-pressure area is formed below the movable base plate (6), thereby causing the overall airflow to generate a downward thrust on the movable base plate (6), dispersing the upward reaction force of the reset spring rod (7) on the movable base plate (6), and maintaining the stability of the second heat dissipation channel.
8. The pet nucleic acid isothermal detection device with constant temperature regulation and control according to claim 1, characterized in that: The heating and insulation component (3) includes an insulation layer (31) wrapped around the outside of the reaction chamber (2), a heating tube (32) is provided inside the insulation layer (31), and a temperature sensor (33) is provided inside the insulation layer (31).
9. The pet nucleic acid isothermal detection device with constant temperature regulation and control according to claim 8, characterized in that: The chassis (1) is equipped with a temperature control system. The heating tube (32), temperature sensor (33), fan (8) and refrigeration unit (9) are all electrically connected to the temperature control system.
10. The pet nucleic acid isothermal detection device with constant temperature regulation and control according to claim 9, characterized in that: The temperature control system uses a PID temperature control algorithm.