Beauty instrument efficacy evaluation device and beauty instrument efficacy evaluation method

Abstract

The invention discloses a beauty instrument efficacy evaluation device and a beauty instrument efficacy evaluation method, the beauty instrument efficacy evaluation device comprises: a cell culture box, the cell culture box comprises two culture group boxes, the culture group boxes are used for culturing test cells; the beauty instrument to be tested is arranged above the cell culture box and is used for performing beauty treatment on the test cells; the beauty instrument to be detected is hung on the hanging moving assembly, and the hanging moving assembly is used for driving the beauty instrument to be detected to move. The beauty instrument efficacy evaluation device can replace a traditional in-vivo test with an in-vitro cell culture system, does not need to depend on living animals or human subjects, and avoids the problem of time consumption of experiment object screening, preparation and long observation period in the in-vivo experiment; and meanwhile, the problems of difficulty in testee recruitment and high experiment cost in a human body experiment are solved.

Description

Technical Field

[0001] This invention relates to the field of beauty device efficacy evaluation technology, specifically to a beauty device efficacy evaluation device and a beauty device efficacy evaluation method. Background Technology

[0002] With the rapid development of beauty devices, various types of beauty devices have emerged on the market, such as radio frequency beauty devices, phototherapy beauty devices, and ultrasonic beauty devices. These beauty devices have effects such as promoting collagen production, improving skin elasticity, and reducing wrinkles.

[0003] Traditional methods for evaluating the efficacy of beauty devices primarily rely on in vivo testing. In vivo testing involves directly applying the device to live animals or humans to observe and assess its effects on skin tissue. While this method provides a relatively direct reflection of the device's effectiveness in real-world applications, it has several drawbacks. Firstly, in vivo testing requires significant time for subject selection, preparation, and subsequent observation. Secondly, human trials face challenges such as difficulty in recruiting participants and high costs. Summary of the Invention

[0004] This invention aims to solve at least one of the technical problems existing in the prior art. To this end, this invention proposes a device for evaluating the efficacy of a beauty instrument, which can replace traditional in vivo experiments with an in vitro cell culture system, without relying on live animals or human subjects, thus avoiding the time-consuming problems of subject selection, preparation, and long observation periods in in vivo experiments.

[0005] The present invention also proposes a method for evaluating the efficacy of a beauty instrument using the aforementioned beauty instrument efficacy evaluation device.

[0006] A beauty device efficacy evaluation apparatus according to a first aspect of the present invention includes: A cell culture box, comprising 2N culture boxes, where N is a positive integer, wherein the culture boxes are used to culture test cells; A beauty device to be tested, located above the cell culture box, is used to perform beauty treatment on the test cells; A suspension moving component is provided, on which the beauty device under test is suspended, and the suspension moving component is used to move the beauty device under test.

[0007] According to an embodiment of the present invention, a device for evaluating the efficacy of a beauty instrument has at least the following beneficial effects: This application uses two culture boxes within a cell culture box to culture test cells. The beauty instrument to be tested is placed above the cell culture box and moved by a suspension and moving component to perform cosmetic treatment on the test cells. This replaces traditional in vivo experiments with an in vitro cell culture system, eliminating the need for live animals or human subjects. This avoids the time-consuming problems of subject selection, preparation, and long observation periods in in vivo experiments, while also solving the difficulties of subject recruitment and high costs in human experiments. Furthermore, moving the beauty instrument to be tested by the suspension and moving component to perform cosmetic treatment on the test cells improves testing efficiency, reduces manual intervention, avoids operational errors, ensures consistent treatment conditions each time, and improves testing accuracy.

[0008] According to some embodiments of the present invention, the two culture boxes are divided into a control group box and a treatment group box, and in a first direction, the control group box and the treatment group box have a height difference.

[0009] According to some embodiments of the present invention, the cell culture box further includes a cover plate with an opening area, the opening area corresponding one-to-one with the culture tank of the treatment box, so as to facilitate the beauty instrument to be tested to perform beauty treatment on the treatment box.

[0010] According to some embodiments of the present invention, the cell culture box further includes a partition, a connecting post is provided at the bottom of the partition, a first connecting hole is provided on the cover plate, a second connecting hole is provided on the control group box, and the connecting post passes through the first connecting hole and is inserted into the second connecting hole.

[0011] According to some embodiments of the present invention, each of the culture boxes includes a plurality of culture tanks, and the plurality of culture tanks are arranged in a matrix.

[0012] According to some embodiments of the present invention, the suspended moving assembly includes a first moving assembly, a support frame, a second moving assembly, and a telescopic assembly. The first moving assembly is arranged along a second direction, which is perpendicular to the first direction, and the support frame is connected to the moving end of the first moving assembly. The second movable component is disposed along a third direction, which is perpendicular to the first direction and the second direction respectively, and the second movable component is disposed on the top of the support frame; The telescopic component is arranged along a first direction, the telescopic component is connected to the moving end of the second moving component, and the beauty instrument to be tested is connected to the telescopic end of the telescopic component.

[0013] A method for evaluating the efficacy of a beauty device according to a second aspect of the present invention, employing a device for evaluating the efficacy of a beauty device according to a first aspect of the present invention, includes the following steps: Step S100: Culture test cells in the control group box and the treatment group box of the cell culture cassette, respectively; Step S200: Set the processing parameters of the beauty device to be tested; Step S300: Set the movement parameters of the intelligent control system; Step S400: Install the beauty device to be tested on the suspended moving assembly; Step S500: The intelligent control system controls the suspended moving component to move above the cell culture box to perform cosmetic treatment on the treatment box; Step S600: Dynamically monitor the changes in biomarkers in the control group box and the treatment group box using a multispectral imaging system to obtain test data; Step S700: The test data is processed and analyzed by the data processing module to generate a beauty device efficacy evaluation report.

[0014] The method for evaluating the efficacy of a beauty device according to embodiments of the present invention has at least the following beneficial effects: By simultaneously culturing test cells in the control group box and the treatment group box, compared with the complexity of subject selection in traditional in vivo experiments, this application can ensure data reliability and save labor costs. Furthermore, by setting the treatment parameters of the beauty device under test and the movement parameters of the suspended moving component, the operating modes of the beauty device under different usage scenarios can be simulated, improving the applicability of the test.

[0015] According to some embodiments of the present invention, the culture of test cells in the control group box and the treatment group box of the cell culture cassette includes: The target cells were placed in the culture tanks of the control group box and the treatment group box. A zebrafish embryo is placed on top of the target cell, and the target cell and the zebrafish embryo are combined to form the test cell.

[0016] According to some embodiments of the present invention, setting the movement parameters of the intelligent control system includes: Set the movement parameters to scan height, movement speed, movement path, number of loops, and interval time.

[0017] According to some embodiments of the present invention, the step of dynamically monitoring changes in biomarkers in the control group box and the treatment group box includes: The changes in the biomarkers were set as the wilting rate of the horsefish tail fin, the area of ​​melanin deposition, the amount of collagen secreted by cells, and the activity of tyrosinase.

[0018] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0019] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein: Figure 1 This is a schematic diagram of the structure of the beauty device efficacy evaluation device according to the first aspect of the present invention; Figure 2 This is an exploded view of the beauty device efficacy evaluation apparatus according to a first aspect embodiment of the present invention; Figure 3 This is a schematic diagram of the structure of a cell culture box according to a first aspect embodiment of the present invention; Figure 4 This is an exploded view of the cell culture box according to a first aspect embodiment of the present invention; Figure 5 This is a partial structural schematic diagram of a cell culture box according to a first aspect embodiment of the present invention; Figure 6 This is a flowchart illustrating the efficacy evaluation method of a beauty device according to a second aspect of the present invention.

[0020] Reference numerals: 100, cell culture box; 110, culture tank; 120, control group box; 130, treatment group box; 140, cover plate; 141, opening area; 142, first connecting hole; 150, partition plate; 151, connecting column; 200, beauty instrument to be tested; 300, suspension moving component; 310, first moving component; 320, support frame; 330, second moving component; 340, telescopic component; 400, base. Detailed Implementation

[0021] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0022] In the description of this invention, it should be understood that the orientation descriptions, such as up, down, front, back, left, right, etc., are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0023] In the description of this invention, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0024] In the description of this invention, unless otherwise explicitly defined, terms such as "set up," "install," and "connect" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this invention in conjunction with the specific content of the technical solution.

[0025] In the description of this invention, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0026] Reference Figure 1 The first aspect of the present invention provides a device for evaluating the efficacy of a beauty instrument, including a cell culture box 100, a beauty instrument to be tested 200, and a suspension and movement component 300.

[0027] Specifically, the cell culture box 100 includes 2N culture boxes, where N is a positive integer, and the culture boxes are used to culture test cells; the beauty device under test 200 is above the cell culture box 100 and is used to perform beauty treatment on the test cells; the suspension moving component 300 suspends the beauty device under test 200 on the suspension moving component 300, and the suspension moving component 300 is used to move the beauty device under test 200.

[0028] It should be noted that cosmetic treatments may involve ultrasound or radiofrequency, and the specific treatment can be designed according to actual needs.

[0029] Reference Figure 3 , Figure 4In some embodiments, each culture box includes several culture tanks 110, arranged in a matrix. Specifically, each culture box has six culture tanks 110 arranged in two rows and three columns to provide sufficient contact area for the beauty device 200 under test. This ensures that the effect of the beauty device can evenly cover the test cells in different culture tanks 110, reducing experimental errors caused by insufficient action area. Furthermore, the beauty device 200 can act on all six culture tanks 110 simultaneously, allowing for the acquisition of six data points in a single test. Compared to the traditional method of testing each culture tank 110 one by one, this improves data acquisition efficiency and allows testing of the beauty device 200 to be performed even with a small culture system, saving manpower and culture costs. Of course, in actual design, the number of culture tanks 110 in each culture box can be designed according to actual needs.

[0030] In some embodiments, N is set to 1. Of course, N can also be set to 2 or 3. In actual design, the size of N can be designed according to actual needs.

[0031] Reference Figure 4 , Figure 5 In some embodiments, the two culture boxes are divided into a control group box 120 and a treatment group box 130. In the first direction, the control group box 120 and the treatment group box 130 have a height difference to avoid cross-contamination of test cells within the treatment group box 130 and the control group box 120. This ensures that the test cells in the control group box 120 are not interfered with by the test cells in the treatment group box, allowing the comparative data from the control group box 120 and the treatment group box 130 to accurately reflect the actual efficacy of the beauty device 200 under test and preventing any impact on the experimental results. Specifically, the height H1 of the control group box 120 is greater than the height H2 of the treatment group box 130, and the height difference between the control group box 120 and the treatment group box 130 is 5mm to 10mm. This ensures isolation while balancing the convenience of experimental operation and the compactness of the device space. It avoids both excessively large height differences affecting the effect of the suspended moving component 300 on the test cells in the treatment group box 130 and excessively small height differences weakening the isolation effect.

[0032] In some embodiments, the cell culture box 100 is made of PC material. PC material has good mechanical strength, chemical stability, and temperature resistance, and can withstand common sterilization processes during cell culture (such as high-temperature and high-pressure steam sterilization) as well as the erosion of various culture media and reagents, ensuring that the cell culture box 100 is reusable and reducing the cost of experimental consumables. In addition, the treatment box 130 is made transparent to facilitate the beauty treatment performed by the beauty instrument 200 under test; the control box 120 is made brown to block light and prevent the light influence of the treatment box 130 on the control box 120 during beauty treatment, so that the comparison data between the control box 120 and the treatment box 130 can truly and accurately reflect the actual effect of the treatment by the beauty instrument 200 under test.

[0033] Reference Figure 3 , Figure 4 In some embodiments, the cell culture box 100 further includes a cover plate 140, which covers the upper surface of the control group box 120. This cover plate prevents contamination of the control group box 120 and also prevents light interference from the beauty device under test 200, improving the accuracy of the test data. Furthermore, the cover plate 140 has opening areas 141 that correspond one-to-one with the culture tanks 110 of the treatment group box 130, facilitating the beauty device under test 200's treatment of the treatment group box 130. Additionally, the opening areas 141 consist of a matrix of through holes, each corresponding one-to-one with the culture tanks 110 of the treatment group box 130. This allows for precise positioning of the beauty device's action area, enabling the beauty device under test 200 to accurately treat the test cells within the treatment group box 130. This ensures that the energy, light radiation, microcurrent, and other treatment conditions of the beauty device are applied evenly and precisely to the test cells in each culture tank 110, avoiding experimental errors caused by deviations in the application area.

[0034] In some embodiments, a 0.22µm filter membrane is disposed on the side of the cover plate 140 near the upper surface of the control group box 120 to further prevent the control group box 120 from being contaminated.

[0035] Reference Figure 3 , Figure 4In some embodiments, the cell culture box 100 further includes a partition 150, with a connecting post 151 at the bottom of the partition 150, a first connecting hole 142 in the cover plate 140, and a second connecting hole in the control group box 120. The connecting post 151 passes through the first connecting hole 142 and is inserted into the second connecting hole, thereby placing the partition 150 at the top of the partition 150. The partition 150 can further enhance the light-blocking effect and further prevent the light generated by the beauty device 200 under test from affecting the control group box 120. Of course, a detachable connection method, such as a snap-fit ​​connection between the partition 150 and the cover plate 140, can also be designed. In actual design, the connection method between the partition 150, the cover plate 140, and the control group box 120 can be designed according to actual needs.

[0036] In some embodiments, multiple cell culture boxes 100 can be configured, arranged along a third direction to simulate different parts of the face. For example, two, three, or four cell culture boxes 100 can be configured. In actual design, the number and arrangement of cell culture boxes 100 can be designed according to actual needs.

[0037] Reference Figure 1 , Figure 2 In some embodiments, the suspended moving assembly 300 includes a first moving assembly 310, a support frame 320, a second moving assembly 330, and a telescopic assembly 340. The first moving assembly 310 is arranged along a second direction, which is perpendicular to the first direction. The support frame 320 is disposed on top of the first moving assembly 310 and connected to the moving end of the first moving assembly 310, enabling the first moving assembly 310 to drive the support frame 320 to move along the second direction. The second moving assembly 330 is arranged along a third direction, which is perpendicular to both the first and second directions. The second moving assembly 330 is disposed on top of the support frame 320. The telescopic assembly 340 is arranged along the first direction and connected to the moving end of the second moving assembly 330, enabling the second moving assembly 330 to drive the telescopic assembly 340 to move along the third direction.

[0038] Reference Figure 1 It should be noted that the first direction can be set to the Z direction, the second direction can be set to the Y direction, and the third direction can be set to the X direction.

[0039] The first moving component 310 moves along a second horizontal direction, covering the longitudinal area of ​​the cell culture box 100; the second moving component 330 moves along a third horizontal direction, covering the transverse area of ​​the cell culture box 100; and the telescopic component 340, with its lifting function along a first direction, adjusts the vertical distance between the beauty device and the cell culture box 100. The first moving component 310, the second moving component 330, and the telescopic component 340 form an orthogonal layout, constructing a three-dimensional motion space. This allows the beauty device 200 to be precisely positioned directly above the cell culture box 100, and also enables the beauty device 200 to perform beauty treatments on multiple cell culture boxes 100, saving testing time and improving testing efficiency.

[0040] Reference Figure 1 , Figure 2 In some embodiments, the beauty device efficacy evaluation device also includes a base 400, a cell culture box 100, and a suspension moving assembly 300, all of which are disposed on top of the base 400.

[0041] In some embodiments, the beauty device efficacy evaluation apparatus also includes an intelligent control system, which is wirelessly connected to the suspended moving component 300. The intelligent control system can set the scanning height, moving speed, moving path, number of cycles, and interval time to simulate the cyclical use of the beauty device 200 on the entire face during actual use.

[0042] Specifically, the scanning height is the height to which the beauty device 200 under test descends, driven by the telescopic component 340. Based on the working characteristics of different types of beauty devices 200 under test, the vertical distance between the beauty device 200 under test and the cell culture box 100 is adjusted to facilitate the beauty device 200 under test performing beauty treatments on the processing unit 130 of the cell culture box 100. The moving speed is the moving speed of the first moving component 310, the second moving component 330, and the telescopic component 340, thereby enabling beauty treatments on multiple cell culture boxes 100. The moving path is the path along which the suspended moving component 300 drives the beauty device 200 under test to perform beauty treatments on the cell culture box 100, allowing the beauty device 200 under test to simulate the movement trajectory in different areas of the face during actual use, such as along a second or third direction. The number of cycles is the number of times the suspended moving component 300 drives the beauty device 200 under test to cycle through the cell culture box 100. The interval time is the duration during which the suspended moving component 300 remains stationary while the beauty instrument under test 200 is at the scanning height and processing each cell culture box 100.

[0043] It should be noted that the scanning height, movement speed, movement path, number of cycles and interval time can be set through the intelligent control system, which is existing technology. This invention has not made any improvements to this part, so its principle and process will not be described in detail.

[0044] This embodiment uses two culture cassettes within a cell culture box 100 to culture test cells. The cosmetic device 200 to be tested is placed above the cell culture box 100 and moved by a suspension and movement component 300 to perform cosmetic treatment on the test cells. This replaces traditional in vivo experiments with an in vitro cell culture system, eliminating the need for live animals or human subjects. This avoids the time-consuming issues of subject selection, preparation, and long observation periods in in vivo experiments, while also circumventing the strict ethical restrictions associated with using live animals. It also solves the problems of difficult subject recruitment and high costs in human experiments. Furthermore, moving the cosmetic device 200 to be tested via the suspension and movement component 300 improves testing efficiency, reduces manual intervention, avoids operational errors, ensures consistent treatment conditions each time, and improves test accuracy.

[0045] Reference Figure 6 A second aspect of the present invention provides a method for evaluating the efficacy of a beauty device, employing a beauty device efficacy evaluation apparatus according to a first aspect of the present invention, comprising the following steps: Step S100: Culture test cells in the control group box 120 and the treatment group box 130 of the cell culture box 100, respectively.

[0046] In step S100, target cells are placed in culture tank 110 of both the control group box 120 and the treatment group box 130. Zebrafish embryos, fertilized for 120 hours, are placed on top of the target cells. Specifically, the target cells are seeded in the lower layer of culture tank 110, and the zebrafish embryos are seeded in the upper layer. The combination of target cells and zebrafish embryos forms the test cells. The lower layer of target cells provides a structure and metabolic basis similar to skin tissue, while the upper layer of zebrafish embryos simulates the overall physiological response of a living organism. Compared to a single-cell model, this more realistically reflects the mechanism of action of the beauty device 200 in vivo.

[0047] In step S100, the target cells can be selected from epidermal, dermal, immune, and accessory organ-related cells, such as keratinocytes, melanocytes, and human skin fibroblasts (HSF), to meet the evaluation requirements of different types of beauty devices 200. Keratinocytes, as the main epidermal cells, can directly reflect the impact of beauty devices on the skin barrier function; melanocytes can be used to evaluate the pigmentation regulation effect of whitening beauty devices; and skin fibroblasts are suitable for detecting the collagen synthesis capacity of anti-wrinkle and firming beauty devices. In actual design, the target cells can be selected according to the evaluation requirements of different types of beauty devices.

[0048] Step S200: Set the processing parameters of the beauty instrument 200 to be tested.

[0049] In step S200, the processing parameters of the beauty device under test 200 include the duration of action. The duration of action on the test cells is controlled by adjusting the power supply and de-energization of the beauty device 200, thus simulating cyclical application to the entire face or a specific area during actual use. For example, if the beauty device 200 uses radiofrequency (RF) technology to simulate cyclical application to the entire face, the duration of action is set to 108 seconds. In actual design, the processing parameters of the beauty device can be designed according to specific needs.

[0050] Step S300: Set the movement parameters of the intelligent control system.

[0051] In step S300, the movement parameters are set according to the evaluation requirements of different types of beauty instruments, and the movement parameters are set as scanning height, movement speed, movement path, number of cycles and interval time.

[0052] For example, phototherapy beauty devices need to maintain a certain irradiation distance to ensure that the light evenly covers the test cells. By adjusting the telescopic component 340, the vertical distance between the beauty device 200 under test and the cell culture box 100 can be set to 5cm. This can avoid damage to the test cells due to excessive light intensity caused by too close a distance, and also ensure that the light energy can effectively act on the test cells. On the other hand, microcurrent beauty devices need to be close to the cell surface. The scanning height can be set to 1cm to ensure current conduction efficiency and improve the reliability of experimental results.

[0053] In addition, the speed parameter setting can be adjusted as follows: for beauty devices 200 that require rapid movement (such as high-frequency vibration devices), the speed of the first moving component 310 and the second moving component 330 can be set to 10cm / s to quickly cover multiple cell culture boxes 100 and increase the test throughput; for radiofrequency beauty devices that require slow action, the speed can be reduced to 3cm / s to ensure that energy is fully transferred to the cells and to avoid uneven processing due to excessive movement.

[0054] Additionally, the movement path can be set to a linear path to simulate the reciprocating movement of the beauty device 200 on the face from the forehead to the chin, making the experimental data closer to the actual application effect. For example, taking an anti-aging radiofrequency beauty device as an example, setting the number of cycles to 3 with an interval of 20 seconds between each cycle can simulate the scenario of multiple and intermittent use in daily skincare, and observe the long-term response of test cells under periodic stimulation; for a whitening phototherapy device, the number of cycles can be increased to 5, and the interval shortened to 10 seconds to observe the immediate reaction of test cells under continuous light exposure.

[0055] In step 300, multiple sets of movement parameters of the intelligent control system can be set at once through the program, allowing the suspended moving component 300 to automatically complete the positioning, processing, and resetting of the beauty instrument according to preset instructions, thereby improving testing efficiency. For example, in one experiment, three different scanning heights, two moving speeds, and four moving paths can be set simultaneously to differentiate six cell culture boxes 100, quickly obtaining a large amount of comparative data.

[0056] Step S400: Install the beauty device to be tested 200 on the suspended moving component 300.

[0057] Step S500: The intelligent control system controls the suspended moving component 800 to move above the cell culture box 100 to perform cosmetic treatment on the treatment box 130.

[0058] Step S600: Dynamically monitor the changes in biomarkers in the control group box 120 and the treatment group box 130 using a multispectral imaging system to obtain test data.

[0059] In step S600, the changes in biomarkers in the control group box 120 and the treatment group box 130 can be monitored using a multispectral imaging system. These biomarker changes are set as zebrafish tail fin wrinkling rate, melanin deposition area, cellular collagen secretion, and tyrosinase activity. The multispectral imaging system utilizes the specific penetration and reflection characteristics of different wavelengths of light into biological tissues to non-invasively and at high resolution capture the microscopic changes in target cells and zebrafish embryos. For example, by irradiating with specific wavelengths of light, the fluorescent labeling of intracellular collagen can be accurately identified, and its secretion changes can be quantified. Using spectral analysis technology, melanin deposition areas can be distinguished from normal tissue, and area changes can be accurately calculated. Simultaneously, continuous dynamic monitoring of the same test sample can be achieved, completely recording the biological response trajectory during the action of the beauty device 200. In addition, the zebrafish tail fin wrinkling rate serves as a direct indicator of zebrafish embryonic developmental toxicity, enabling rapid detection of whether the tested beauty device 200 poses a risk of cytotoxicity or teratogenicity. The area of ​​melanin deposition is directly related to tyrosinase activity, which is in turn related to the effectiveness of whitening beauty devices. The area of ​​melanin deposition quantifies the degree of pigment reduction, while tyrosinase activity reveals the whitening mechanism from the perspective of enzyme activity. The amount of collagen secreted by cells is a core evaluation indicator for anti-aging beauty devices, reflecting their ability to promote skin repair and firming.

[0060] In step S600, the zebrafish tail fin wrinkling rate was calculated by statistically analyzing the tail fin morphology of zebrafish embryos using image analysis software, and the proportion of wrinkled embryos was determined. The melanin deposition area was measured by exciting melanin within melanocytes with specific wavelength light and measuring its deposition area within the culture tank 110. Cellular collagen secretion was quantified by performing collagen-specific fluorescent staining on human skin fibroblasts and measuring the fluorescence intensity. Tyrosinase activity was calculated by spectrophotometry, measuring the absorbance of the tyrosinase-catalyzed substrate product in the cell lysate, and determining the enzyme activity.

[0061] It should be noted that monitoring changes in biomarkers in the control group box 120 and the treatment group box 130 using a multispectral imaging system is existing technology. This invention has also made improvements to this part, so its principles and processes will not be described in detail here.

[0062] Step S700: The test data is processed and analyzed by the data processing module to generate a beauty device efficacy evaluation report.

[0063] In step S700, the test data acquired by the multispectral imaging system is transmitted to the data processing module. This module possesses automated data integration and analysis capabilities, enabling it to quickly summarize biomarker change data acquired by the multispectral imaging system, avoiding errors and omissions that may occur during manual statistical analysis. Furthermore, the data processing module performs data standardization and statistical analysis using preset algorithms, automatically identifying differences between the treatment group box 130 and the control group box 120, thus improving data analysis efficiency. Simultaneously, the data processing module supports visualization, converting test values ​​into intuitive charts such as line graphs, bar charts, and heatmaps. For example, a bar chart can be used to compare the differences in melanin deposition area between the treatment group box 130 and the control group box 120.

[0064] It should be noted that the test data is processed and analyzed using the data processing module, which is in accordance with existing technology. This invention does not make any improvements to the stinger part, so its principle and process will not be described in detail.

[0065] In step S700, the beauty device efficacy evaluation report includes basic report information, data results and analysis, visualization charts, and conclusions. The beauty device efficacy evaluation report clearly presents the differences in the effects of the tested beauty device 200 under different processing parameters, providing data support for optimizing product design.

[0066] The second embodiment of the beauty device efficacy evaluation method of the present invention, by simultaneously culturing test cells in the control group box 120 and the treatment group box 130, ensures data reliability and saves labor costs compared to the complexity of subject selection in traditional in vivo experiments. Furthermore, by setting the processing parameters of the beauty device 200 under test and the movement parameters of the suspended moving component 300, the operating modes of the beauty device under different usage scenarios can be simulated, improving the applicability of the test.

[0067] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof can be combined with each other unless otherwise specified.

Claims

1. A device for evaluating the efficacy of a beauty instrument, characterized in that, include: A cell culture box, comprising 2N culture boxes, where N is a positive integer, wherein the culture boxes are used to culture test cells; A beauty device to be tested, located above the cell culture box, is used to perform beauty treatment on the test cells; A suspension moving component is provided, on which the beauty device under test is suspended, and the suspension moving component is used to move the beauty device under test.

2. The beauty instrument efficacy evaluation device according to claim 1, characterized in that, The culture box is divided into a control group box and a treatment group box, and in a first direction, the control group box and the treatment group box have a height difference.

3. The beauty instrument efficacy evaluation device according to claim 2, characterized in that, The cell culture box also includes a cover plate with an opening area that corresponds one-to-one with the culture tank of the treatment box, so that the beauty instrument to be tested can perform beauty treatment on the treatment box.

4. The beauty instrument efficacy evaluation device according to claim 3, characterized in that, The cell culture box also includes a partition, a connecting post at the bottom of the partition, a first connecting hole in the cover plate, and a second connecting hole in the control group box. The connecting post passes through the first connecting hole and is inserted into the second connecting hole.

5. The beauty instrument efficacy evaluation device according to claim 1, characterized in that, Each of the culture boxes includes several culture tanks, and the several culture tanks are arranged in a matrix.

6. The beauty instrument efficacy evaluation device according to claim 2, characterized in that, The suspended moving component includes a first moving component, a support frame, a second moving component, and a telescopic component. The first moving component is arranged along a second direction, which is perpendicular to the first direction, and the support frame is connected to the moving end of the first moving component. The second movable component is disposed along a third direction, which is perpendicular to the first direction and the second direction respectively, and the second movable component is disposed on the top of the support frame; The telescopic component is arranged along the first direction, the telescopic component is connected to the moving end of the second moving component, and the beauty instrument to be tested is connected to the telescopic end of the telescopic component.

7. A method for evaluating the efficacy of a beauty device, employing the beauty device efficacy evaluation apparatus as described in any one of claims 1 to 6, characterized in that, Includes the following steps: Step S100: Culture test cells in the control group box and the treatment group box of the cell culture cassette, respectively; Step S200: Set the processing parameters of the beauty device to be tested; Step S300: Set the movement parameters of the intelligent control system; Step S400: Install the beauty device to be tested on the suspended moving assembly; Step S500: The intelligent control system controls the suspended moving component to move above the cell culture box to perform cosmetic treatment on the treatment box; Step S600: Dynamically monitor the changes in biomarkers in the control group box and the treatment group box using a multispectral imaging system to obtain test data; Step S700: The test data is processed and analyzed by the data processing module to generate a beauty device efficacy evaluation report.

8. The method for evaluating the efficacy of a beauty device according to claim 7, characterized in that, The process of culturing test cells in the control group and treatment group boxes of the cell culture cassette includes: The target cells were placed in the culture tanks of the control group box and the treatment group box. A zebrafish embryo is placed on top of the target cell, and the target cell and the zebrafish embryo are combined to form the test cell.

9. The method for evaluating the efficacy of a beauty device according to claim 7, characterized in that, The movement parameters of the intelligent control system include: Set the movement parameters to scan height, movement speed, movement path, number of loops, and interval time.

10. The method for evaluating the efficacy of a beauty device according to claim 7, characterized in that, The dynamic monitoring of biomarker changes in the control group box and the treatment group box using a multispectral imaging system includes: The changes in the biomarkers were set as the wilting rate of the horsefish tail fin, the area of ​​melanin deposition, the amount of collagen secreted by cells, and the activity of tyrosinase.

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