A microfluidic method for preparing a nano-liposome emulsion with moisturizing efficacy and a preparation method thereof

Abstract

This invention belongs to the field of cosmetic technology, specifically disclosing a moisturizing nanoliposome emulsion prepared by microfluidic method and its preparation method, aiming to solve the problems of poor stability of active ingredients, low transdermal absorption efficiency, single efficacy, and uneven particle size in the preparation process of liposomes in existing moisturizing cosmetics. This nanoliposome emulsion contains glyceryl glucoside, acetylglucosamine, Tremella fuciformis polysaccharide, Myrothamnus fruticosa leaf / stem extract, Artemisia annua extract, Lactobacillus fermentation product, Lysimachia christinae oil, hydrogenated lecithin, and phosphatidylcholine; this emulsion has excellent moisturizing, soothing, and repairing effects, and can be used to prepare skin care products such as aqueous solutions, emulsions, and creams, with the advantages of high utilization rate of active ingredients and good stability.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more specifically, to a method for preparing a moisturizing nanoliposome emulsion using a microfluidic method. Background Technology

[0002] In the field of cosmetic moisturizing, consumers' demands for products have evolved from "simple hydration" to a combination of effects including "long-lasting moisturizing + barrier repair + soothing and anti-inflammatory." However, existing technologies still have the following key shortcomings:

[0003] Limited efficacy and poor stability of active ingredients: Traditional moisturizing products often rely on a single moisturizer, lacking soothing and repairing ingredients for synergy. Furthermore, active ingredients are easily degraded by temperature and light, leading to a decrease in efficacy. At the same time, some active ingredients have low transdermal absorption efficiency, making it difficult for them to penetrate deep into the stratum corneum to exert their effects, and they only provide temporary moisturization on the surface.

[0004] Defects in liposome preparation processes: Liposomes are commonly used carriers to improve the stability and transdermal permeability of active ingredients, but traditional preparation methods (such as thin film dispersion and ultrasonication) have significant shortcomings: Thin film dispersion easily forms liposomes with uneven particle size, leading to easy stratification of the system; Although ultrasonication can reduce particle size, the local temperature is too high, which can easily damage the active ingredients; In addition, traditional wall materials often use single lecithin or a combination of lecithin and cholesterol, which has a low encapsulation rate for various active ingredients with large polarity differences, and is prone to leakage of active ingredients.

[0005] Insufficient overall product synergy: In existing products, moisturizing, soothing, and repairing ingredients are often simply mixed without considering the interaction between them; at the same time, the carrier process is not compatible with the characteristics of the ingredients, resulting in the final product's efficacy failing to achieve synergistic effects and making it difficult to meet the needs of sensitive and dry skin for integrated moisturizing, soothing, and repair. Summary of the Invention

[0006] In view of the shortcomings of the prior art, the purpose of this invention is to provide a nanoliposome emulsion and its preparation method, which can not only achieve synergistic effect of active ingredients, but also improve the stability of carrier process adaptation, and at the same time, improve transdermal absorption and promote efficacy.

[0007] To achieve the above objectives, the present invention discloses the following technical solutions:

[0008] In a first aspect, the present invention provides a nanoliposome emulsion with moisturizing effects, wherein the nanoliposome emulsion contains glyceryl glucoside, acetylglucosamine, Tremella fuciformis polysaccharide, Myrothamnus fruticosa leaf / stem extract, Artemisia annua extract, Lactobacillus fermentation product, Pleurotus ostreatus oil, hydrogenated lecithin, and phosphatidylcholine.

[0009] Preferably, the nanoliposome emulsion contains the following components in parts by weight:

[0010] 2-8 parts of glycerol glucoside;

[0011] 1-3 parts of acetylglucosamine;

[0012] 1-5 parts of Tremella polysaccharide;

[0013] Myrothamnus flabellifolia leaf / stem extract 0.1-1 part;

[0014] Artemisia annua extract 1-5 parts;

[0015] 5-10 parts of Lactobacillus fermentation product;

[0016] 1-4 parts of flat-kernel wood oil;

[0017] 1-12 parts of hydrogenated lecithin;

[0018] Phosphatidylcholine 1-2 parts.

[0019] In a second aspect, the present invention provides a method for preparing the nanoliposome emulsion described in the first aspect, the method comprising the following steps:

[0020] Step 1. Aqueous phase pretreatment: Glyceryl glucoside, acetylglucosamine, Tremella fuciformis polysaccharide, Myrothamnus fruticosa leaf / stem extract, Artemisia annua extract, and Lactobacillus fermentation product are added to deionized water and stirred to form an aqueous phase solution;

[0021] Step 2. Wall material pretreatment: Add flat-shell wood oil, hydrogenated lecithin, and phosphatidylcholine to anhydrous ethanol and mix until completely dissolved, then remove the anhydrous ethanol to form a wall material film;

[0022] Step 3. Add the aqueous solution to the wall material film and stir to mix, forming a primary emulsion system;

[0023] Step 4. The colostrum system is circulated 2-7 times under a high-pressure microfluidic device to obtain the moisturizing nanoliposome emulsion.

[0024] Preferably, in step 1, the material is added to deionized water and mixed at 45-55°C and 200-300 rpm for 20-30 minutes to form an aqueous solution.

[0025] Preferably, in step 2, the flat-shell wood oil, hydrogenated lecithin, and phosphatidylcholine are added to anhydrous ethanol and mixed, stirred at 25°C and 200-300 rpm until completely dissolved, and then the anhydrous ethanol is removed by rotary evaporation at 40-45°C to form a wall material film.

[0026] More preferably, the flat-shell wood oil, hydrogenated lecithin, and phosphatidylcholine are added to anhydrous ethanol in a mass ratio of (1-2):(5-6):1.

[0027] Preferably, in step 3, the aqueous solution is slowly added to the wall material film at a dropping rate of 10 mL / min, and mixed for 20-30 min under stirring at 50-60℃ and 500-1000 rpm to form a primary emulsion system.

[0028] Preferably, in step 4, the colostrum system is first introduced into a high-pressure microfluidic device, the pressure is set to 70-90 MPa, and the system is circulated 2-3 times; then the pressure is adjusted to 100-130 MPa, and the system is circulated 3-4 times. During the process, the system temperature is controlled to be ≤40℃ to obtain the moisturizing nanoliposome emulsion.

[0029] Thirdly, the present invention provides the application of the nanoliposome emulsion described in the first aspect in the preparation of skin care products with moisturizing, soothing and / or repairing effects.

[0030] Preferably, the skincare product is an aqueous solution, emulsion, cream, ointment, serum, or mask.

[0031] In this invention:

[0032] Glyceryl glucoside, as a core moisturizer, has both water-absorbing and water-locking capabilities. It can absorb moisture from the environment and form a moisturizing film on the skin surface, while promoting the hydration of the stratum corneum, which is the basis for long-lasting moisturizing.

[0033] Acetyl glucosamine has both moisturizing and stratum corneum repairing effects. It can gently promote the metabolism of the stratum corneum, improve the tightness of the stratum corneum arrangement, enhance the skin barrier's ability to retain moisture, and at the same time assist other active ingredients in transdermal absorption.

[0034] As a natural high-molecular-weight moisturizer, Tremella polysaccharide contains a large number of hydroxyl groups in its molecular structure. It can bind several times its own weight of water to form a breathable moisturizing film on the skin surface, preventing water evaporation. At the same time, it has a certain soothing effect and reduces skin irritation caused by dryness.

[0035] The core soothing ingredient, Myrothamnus flabellifolia leaf / stem extract, contains flavonoids and polyphenols, which can inhibit the release of inflammatory factors and relieve redness and itching caused by dryness and external irritation, while enhancing the skin's tolerance to environmental stress. Artemisia annua extract, as a synergistic soothing ingredient, has anti-inflammatory and antibacterial effects, which can further inhibit inflammatory responses. Combined with Myrothamnus flabellifolia leaf / stem extract, it forms a "double soothing" effect, which is especially suitable for sensitive skin.

[0036] Lactobacillus fermentation products can regulate the skin's surface microecological balance, promote the synthesis of ceramides in the stratum corneum, enhance the integrity of the skin barrier, reduce transepidermal water loss, and improve the skin's own moisturizing ability from the root.

[0037] Flat-kernel wood oil is a natural oil carrier that combines moisturizing and wall material auxiliary functions. It can replenish skin lipids and improve dry skin. At the same time, its weak polarity and high antioxidant properties not only regulate the hydrophilicity and hydrophobicity of the wall material and improve the encapsulation rate of weakly polar active ingredients, but also inhibit the risk of wall material oxidation and improve stability.

[0038] Hydrogenated lecithin, as the core wall material matrix, has good biocompatibility and emulsification stability. It can self-assemble in water to form a lipid bilayer to encapsulate active ingredients. Compared with ordinary lecithin, hydrogenated lecithin has a higher saturation, a more stable wall film structure, is less prone to oxidation, and extends the product's shelf life.

[0039] Phosphatidylcholine, as a wall material modifier, can enhance the fluidity and flexibility of hydrogenated lecithin bilayer, prevent liposomes from breaking due to external forces (such as high-pressure homogenization, microfluidic treatment, etc.), and at the same time improve the compatibility of liposomes with the stratum corneum of the skin, promoting the transdermal release of active ingredients.

[0040] The beneficial effects of this invention are:

[0041] 1. The nanoliposome emulsion provided by this invention effectively increases the moisture content of the stratum corneum and reduces transepidermal water loss through the synergistic combination of moisturizing, soothing and repairing ingredients. It has significant moisturizing and barrier repair effects. At the same time, the liposome emulsion can effectively inhibit the generation of inflammatory factors and has excellent soothing and anti-inflammatory effects, which can meet the multiple needs of dry skin and sensitive skin.

[0042] 2. The specific wall material combination of hydrogenated lecithin, phosphatidylcholine and flat-shell wood oil provided by the present invention, combined with the micro-jet segmented processing technology, makes the liposomes uniform in size and have excellent stability, avoiding their degradation or oxidation and extending the duration of efficacy. Attached Figure Description

[0043] Figure 1 The chart shows a comparison of the stability index (TSI) between Example 2 and Comparative Examples 6-9. Detailed Implementation

[0044] The present invention will be described in detail below with reference to specific embodiments and examples, thereby making the advantages and various effects of the present invention more clearly apparent. Those skilled in the art should understand that these specific embodiments and examples are for illustrative purposes only and are not intended to limit the present invention.

[0045] Throughout this specification, unless otherwise specified, the terminology used herein should be understood as having the meaning commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the event of any conflict, this specification shall prevail.

[0046] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be purchased from the market or prepared by existing methods.

[0047] In this invention:

[0048] Glyceryl glucoside: purchased from Guangdong Miqi New Materials Co., Ltd.;

[0049] Acetyl glucosamine: purchased from Shanghai Zibang Biopharmaceutical Co., Ltd.;

[0050] Tremella polysaccharide: purchased from Guangdong Zhuangshu Fine Chemical Co., Ltd.;

[0051] Myrothamnus flabellifolia leaf / stem extract: purchased from RAHN AG;

[0052] Artemisia annua extract: purchased from Nanjing Institute of Comprehensive Utilization of Wild Plants;

[0053] Lactobacillus fermentation products: purchased from Youkemeigu (Shandong) Biotechnology Co., Ltd.

[0054] Preparation of moisturizing nanoliposomes

[0055] Example 1

[0056] Step 1: Raw material pretreatment

[0057] Aqueous phase pretreatment: 2g glyceryl glucoside, 1g acetylglucosamine, 1g Tremella fuciformis polysaccharide, 0.1g Myrothamnus fruticosa leaf / stem extract, 1g Artemisia annua extract, and 5g Lactobacillus fermentation product were added to 500mL of deionized water and mixed at 45℃ and 200rpm for 20min to form an aqueous phase solution.

[0058] Wall material pretreatment: Add 1g of flat-shell wood oil, 5g of hydrogenated lecithin and 1g of phosphatidylcholine to 500mL of anhydrous ethanol and mix. Stir at 25℃ until completely dissolved, then transfer to a vacuum rotary evaporator to remove the anhydrous ethanol at 40℃ to form a wall material film.

[0059] Step 2: Colostrum Preparation

[0060] The aqueous solution was slowly added to the wall material film at a dropping rate of 10 mL / min and mixed for 30 min at 50 °C and 500 rpm to form a primary emulsion system.

[0061] Step 3: High-pressure micro-jet segmented processing

[0062] Primary dispersion: The colostrum system was passed into a high-pressure microfluidic device (model: MF7125BP-30K), the pressure was set to 70MPa, and the system was circulated 3 times. At this time, the particle size of the system was reduced to 200-300nm.

[0063] Secondary refinement: Adjust the microjet pressure to 100MPa, cycle the process 4 times, and control the system temperature to ≤40℃ through the equipment cooling system during the process. Finally, obtain a nanoliposome system with a particle size of 50-100nm, and obtain the moisturizing nanoliposome emulsion.

[0064] Example 2

[0065] Step 1: Raw material pretreatment

[0066] Aqueous phase pretreatment: 6g glyceryl glucoside, 2.5g acetylglucosamine, 3g Tremella fuciformis polysaccharide, 0.7g Myrothamnus fruticosa leaf / stem extract, 4g Artemisia annua extract, and 8g Lactobacillus fermentation product were added to 500mL of deionized water and mixed at 50℃ and 300rpm for 30min to form an aqueous phase solution.

[0067] Wall material pretreatment: Add 3g of flat-shell wood oil, 10g of hydrogenated lecithin and 2g of phosphatidylcholine to 500mL of anhydrous ethanol and mix. Stir at 25℃ until completely dissolved, then transfer to a vacuum rotary evaporator to remove the anhydrous ethanol at 45℃ to form a wall material film.

[0068] Step 2: Colostrum Preparation

[0069] The aqueous solution was slowly added to the wall material film at a dropping rate of 10 mL / min and mixed for 20 min at 55 °C and 700 rpm to form a primary emulsion system.

[0070] Step 3: High-pressure micro-jet segmented processing

[0071] Primary dispersion: The colostrum system was passed into a high-pressure microfluidic device (model: MF7125BP-30K), the pressure was set to 80MPa, and the system was circulated twice. At this time, the particle size of the system was reduced to 200-300nm.

[0072] Secondary refinement: Adjust the microjet pressure to 120MPa, cycle the process 3 times, and control the system temperature to ≤40℃ through the equipment cooling system during the process. Finally, obtain a nanoliposome system with a particle size of 50-100nm, and obtain the moisturizing nanoliposome emulsion.

[0073] Example 3

[0074] Step 1: Raw material pretreatment

[0075] Aqueous phase pretreatment: 8g glyceryl glucoside, 3g acetylglucosamine, 5g Tremella fuciformis polysaccharide, 1g Myrothamnus fruticosa leaf / stem extract, 5g Artemisia annua extract, and 10g Lactobacillus fermentation product were added to 500mL of deionized water and mixed at 55℃ and 300rpm for 30min to form an aqueous phase solution.

[0076] Wall material pretreatment: Add 4g of flat-shell wood oil, 12g of hydrogenated lecithin and 2g of phosphatidylcholine to 500mL of anhydrous ethanol and mix. Stir at 25℃ until completely dissolved, then transfer to a vacuum rotary evaporator to remove the anhydrous ethanol at 45℃ to form a wall material film.

[0077] Step 2: Colostrum Preparation

[0078] The aqueous solution was slowly added to the wall material film at a dropping rate of 10 mL / min and mixed for 20 min at 60 °C and 1000 rpm to form a primary emulsion system.

[0079] Step 3: High-pressure micro-jet segmented processing

[0080] Primary dispersion: The colostrum system was passed into a high-pressure microfluidic device (model: MF7125BP-30K), the pressure was set to 90MPa, and the system was circulated twice. At this time, the particle size of the system was reduced to 200-300nm.

[0081] Secondary refinement: Adjust the microjet pressure to 130MPa, cycle the process 3 times, and control the system temperature to ≤40℃ through the equipment cooling system during the process, finally obtain a nanoliposome system with a particle size of 50-100nm, and obtain the moisturizing nanoliposome emulsion.

[0082] Comparative Example 1

[0083] The preparation of Comparative Example 1 was carried out in accordance with the preparation method of Example 2, except that it lacked glycerol glucoside, while the rest was the same as that of Example 2.

[0084] Comparative Example 2

[0085] Comparative Example 2 was prepared according to the preparation method of Example 2, except that it lacked acetyl glucosamine, otherwise it was the same as Example 2.

[0086] Comparative Example 3

[0087] Comparative Example 3 was prepared according to the preparation method of Example 2, except that it lacked the Myrothamnus flabellifolia leaf / stem extract, otherwise it was the same as Example 2.

[0088] Comparative Example 4

[0089] The preparation of Comparative Example 4 was carried out in accordance with the preparation method of Example 2, except that Artemisia annua extract was not included, otherwise it was the same as Example 2.

[0090] Comparative Example 5

[0091] Comparative Example 5 was prepared according to the preparation method of Example 2, except that it lacked the Lactobacillus fermentation product, otherwise it was the same as Example 2.

[0092] Comparative Example 6

[0093] The preparation of Comparative Example 6 was carried out in accordance with the preparation method of Example 2. The difference from Example 2 was that the hydrogenated lecithin in the wall material pretreatment in step 1 was replaced with lecithin, and the amount was 10g. The rest was the same as in Example 2.

[0094] Comparative Example 7

[0095] Comparative Example 7 was prepared according to the preparation method of Example 2. The difference from Example 2 was that phosphatidylcholine in the wall material pretreatment step 1 was replaced with cholesterol, with an amount of 2g. The rest was the same as in Example 2.

[0096] Comparative Example 8

[0097] The preparation of Comparative Example 8 was carried out in accordance with the preparation method of Example 2. The difference from Example 2 was that the flat-shell wood oil in step 1 wall material pretreatment was omitted, and instead, 12.5g of hydrogenated lecithin and 2.5g of phosphatidylcholine were added to 500mL of anhydrous ethanol and mixed. The rest was the same as in Example 2.

[0098] Comparative Example 9

[0099] The preparation of Comparative Example 9 was carried out in accordance with the preparation method of Example 2. The difference from Example 2 was that the mass of flat-shell wood oil, hydrogenated lecithin and phosphatidylcholine in the wall material pretreatment in step 1 was adjusted to 5g flat-shell wood oil, 5g hydrogenated lecithin and 5g phosphatidylcholine added to 500mL anhydrous ethanol and mixed. The rest was the same as in Example 2.

[0100] Performance testing

[0101] 1. Soothing efficacy test - Inflammatory factor inhibition experiment

[0102] Test samples: Take a certain amount of nanoliposome emulsions from Examples 1-3 and Comparative Examples 1-5, and dilute them to 0.5v / v%, 1v / v%, and 3v / v respectively using cell culture medium to obtain cell culture medium containing a certain concentration of nanoliposome emulsions, which are denoted as test samples 1-8.

[0103] Experimental Methods: RAW264.7 macrophages were used as the research subject. A cellular inflammation model was established by stimulating cells with lipopolysaccharide (LPS), a bacterial endotoxin. Macrophages were seeded into 12-well plates and incubated at 37°C and 5 v / v% CO2 for 24 h. Then, appropriate drugs were added as follows:

[0104] Drug administration: Add the corresponding test sample, and after 2 hours add cell culture medium containing 1 μg / mL LPS. Repeat for 6 wells and record as T1.

[0105] Negative control: Add drug-free cell culture medium (the same volume as the test sample), and after 2 hours, add cell culture medium containing 1 μg / mL LPS. Repeat for 6 wells, denoted as T. 总 ;

[0106] Blank control: The group that does not add LPS but only adds the sample to be tested is denoted as T0.

[0107] After 24 hours of stimulation following the completion of the above drug administration, the supernatant was collected, centrifuged, and analyzed. The release level of the pro-inflammatory cytokine TNF-α in RAW264.7 was analyzed using an ELISA kit.

[0108] The TNF-α inhibition rate is calculated using the following formula:

[0109] TNF-α inhibition rate (%) = [(T 总 -T1) / (T 总 -T0)]×100%.

[0110] A higher TNF-α inhibition rate indicates a better anti-inflammatory and soothing effect of the soothing and moisturizing composition. The TNF-α inhibition test results are shown in Table 1.

[0111] Table 1 TNF-α inhibition rate

[0112]

[0113] Results analysis:

[0114] As shown in Table 1, the TNF-α inhibition rate of Examples 1-3 all increased with increasing sample concentration, indicating that the soothing effect of the active ingredient is concentration-dependent and has a strong anti-inflammatory effect at a concentration of 3v / v%.

[0115] The TNF-α inhibition rates of Comparative Examples 1-5 also showed a certain concentration dependence, but were all inferior to those of Example 2. Among them, Comparative Examples 3 and 4 lacked Myrothamnus flabellifolia leaf / stem extract and Artemisia annua extract, respectively, and had the lowest inflammatory factor inhibition rates, indicating that these two extracts are the core soothing components, and the two synergistically inhibit TNF-α release. Comparative Examples 1, 2, and 5 lacked glycerol glucoside, acetyl glucosamine, and Lactobacillus fermentation products, respectively, which led to a decrease in their performance compared to Example 2. This indicates that glycerol glucoside, acetyl glucosamine, and Lactobacillus fermentation products also participate in the synergistic soothing effect, and the absence of any one of these components will lead to a significant reduction in anti-inflammatory and soothing efficacy.

[0116] 2. Stability Test

[0117] The physical stability of the 6-9 nanoparticle liposome emulsions from Example 2 and Comparative Example 2 was rapidly analyzed using a Turbiscan stability analyzer. 20 mL of sample was placed in a sample vial, the temperature was set to 25°C, and scans were performed every 3 hours for 24 hours. The stability index (TSI, unit: %) was recorded.

[0118] See results Figure 1 .

[0119] Results analysis:

[0120] Example 2 uses a combination of hydrogenated lecithin, phosphatidylcholine, and walnut oil as wall materials in a specific mass ratio, combined with microfluidic segmented treatment. The lowest 24-hour TSI value indicates that the system has uniform particle size, no obvious particle agglomeration or stratification, and excellent stability. The high stability of hydrogenated lecithin, the flexibility of phosphatidylcholine, and the high antioxidant and hydrophilicity-hydrophobicity regulation effects of walnut oil together ensure the stability of the liposome membrane structure and avoid system instability.

[0121] 3. Moisturizing efficacy test

[0122] The moisture content and transepidermal water loss on the inner forearm skin surface of the test subjects were measured to characterize the moisturizing and repairing effects of the nanoliposome emulsions prepared in Examples 1-3 and Comparative Examples 1-5.

[0123] Several subjects aged 35-50 years with dry skin on the inner side of their arms were randomly divided into 8 groups, with 10 subjects in each group (5 males and 5 females). Each group received the nanoliposome emulsions described in Examples 1-3 and Comparative Examples 1-5, at a concentration of 1.8 mg / cm³. 2 The appropriate amount of product was applied once and evenly to the test area on the inner forearm skin of the subject. The stratum corneum moisture content and transepidermal water loss were measured using an MPA580. The test values ​​were recorded before and 10 hours after application of the nanoliposome emulsion. Experimental conditions were: temperature (25±0.5)℃, relative humidity (55±5)%. The rate of change in stratum corneum moisture content (Q) and the rate of change in transepidermal water loss (K) after 10 hours of use were then calculated. The results are shown in Table 2.

[0124] Table 2

[0125] Group Q value of the rate of change of water content in the stratum corneum of the skin K-value of skin transepidermal water loss rate Example 1 16.54 -12.74 Example 2 23.17 -19.43 Example 3 24.82 -20.29 Comparative Example 1 14.57 -10.38 Comparative Example 2 12.65 -8.26 Comparative Example 3 10.08 -6.55 Comparative Example 4 11.37 -7.29 Comparative Example 5 15.13 -11.41

[0126] Results analysis:

[0127] As can be seen from the data in Table 2, the skin stratum corneum moisture content change rate Q value of Examples 1-3 is relatively high, reaching 16.54% to 24.82%, and the transepidermal water loss change rate K value is relatively low, reaching -20.29% to -12.74%, indicating that the nanoliposome emulsion of the present invention has significant moisturizing and barrier repair effects.

[0128] The Q values ​​of Comparative Examples 1-5 were all lower than those of the Example, and the absolute values ​​of their K values ​​were smaller, indicating poorer moisturizing effects. Among them, Comparative Example 3, lacking Myrothamnus flabellifolia leaf / stem extract, and Comparative Example 4, lacking Artemisia annua extract, showed the worst moisturizing effects, indicating that these two extracts make significant contributions to the overall moisturizing and repairing efficacy of the formula. Comparative Example 1, lacking glyceryl glucoside, and Comparative Example 5, lacking Lactobacillus fermentation product, showed better moisturizing effects than the other comparative examples, but still inferior to the Example, indicating that glyceryl glucoside and Lactobacillus fermentation product are key moisturizing ingredients. Overall, this invention achieves excellent moisturizing efficacy through the synergistic effect of multiple active ingredients.

[0129] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A nano-liposome emulsion having a moisturizing effect, characterized by, The nanoliposome emulsion contains the following components by mass fraction: Glycosylglycerol 6-8 parts; Acetylated chitosamine 2.5-3 parts; Tremella polysaccharide 3-5 parts; Myrobalan leaf / stem extract 0.7-1 part; Artemisia annua extract 4-5 parts; Lactobacillus fermentation product 8-10 parts; Flat-core wood oil 3-4 parts; Hydrogenated lecithin 10-12 parts; Phosphatidylcholine 2 parts.

2. The method of claim 1, wherein the nanoliposomal emulsion is prepared by, The preparation method comprises the following steps: Step 1. Water phase pretreatment: glycosylglycerol, acetylated chitosamine, tremella polysaccharide, myrobalan leaf / stem extract, artemisia annua extract, and lactobacillus fermentation product are added to deionized water, stirred and mixed to form a water phase solution; Step 2. Wall material pretreatment: flat-core wood oil, hydrogenated lecithin, and phosphatidylcholine are added to anhydrous ethanol and mixed until completely dissolved, and then the anhydrous ethanol is removed to form a wall material film; Step 3. The water phase solution is added to the wall material film and stirred and mixed to form a preliminary emulsion system; Step 4. The preliminary emulsion system is treated by circulating in a high-pressure microfluidization device for 2-7 times to obtain the moisturizing nanoliposome emulsion.

3. The production method according to claim 2, characterized by, In step 1, after the materials are added to deionized water, they are mixed at 45-55°C and a stirring rate of 200-300 rpm for 20-30 min to form a water phase solution.

4. The preparation method according to claim 2, characterized in that, In step 2, flat-core wood oil, hydrogenated lecithin, and phosphatidylcholine are added to anhydrous ethanol and mixed, and then stirred at 25°C and a stirring rate of 200-300 rpm until completely dissolved, and then anhydrous ethanol is removed by rotary evaporation at 40-45°C to form a wall material film.

5. The preparation method according to claim 2, characterized in that, In step 3, the water phase solution is slowly added to the wall material film at a drop rate of 10 mL / min, and then stirred and mixed at 50-60°C and a stirring rate of 500-1000 rpm for 20-30 min to form a preliminary emulsion system.

6. The preparation method according to claim 2, characterized in that, In step 4, the preliminary emulsion system is first introduced into a high-pressure microfluidization device, the pressure is set to 70-90 MPa, and the system is treated by circulating for 2-3 times; then the pressure is adjusted to 100-130 MPa, and the system is treated by circulating for 3-4 times, and the temperature of the system is controlled to be ≤40°C during the process to obtain the moisturizing nanoliposome emulsion.

7. Use of the nanoliposome emulsion of claim 1 in the preparation of a skin care product with moisturizing, soothing, and / or repairing effects.

8. Use according to claim 7, characterized in that, The skin care product is an aqueous agent, an emulsion, a cream, a paste, or a mask.

9. Use according to claim 7, characterized in that, The skin care product is a serum.

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