Composition, submicron gold ball composition and application of submicron gold ball composition in preparation of skin care products

Abstract

The invention relates to the technical field of biological medicine, in particular to a composition, a submicron gold ball composition and application of the submicron gold ball composition in preparation of skin care products. The composition comprises amino acid polypeptide and a plant extract, the amino acid polypeptide is selected from one or a combination of more than one of acetyl hexapeptide-1, acetyl hexapeptide-8, palmitoyl pentapeptide-4 and snake venom-like peptide; the plant extract is selected from centella asiatica extract and / or lucid ganoderma extract. The submicron gold ball composition is obtained by wrapping the composition by adopting a submicron wrapping technology. The composition disclosed by the invention not only has related effects of amino acid polypeptide, but also has an anti-inflammatory effect; particularly, the submicron gold ball composition not only has good skin infiltration capacity, but also can effectively load active ingredients and realize a slow release effect, so that the stability and the use effect of a skin care product are remarkably improved.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, specifically to a composition, a submicron gold sphere composition, and its application in the preparation of skincare products. Background Technology

[0002] Anti-aging skincare products have become an important part of the cosmetics market. In recent years, an increasing number of studies have shown that peptide compounds, due to their good bioactivity and safety, have demonstrated significant effects in improving skin wrinkles, promoting collagen production, and enhancing skin elasticity, making them an indispensable functional ingredient in high-end skincare products.

[0003] Acetyl hexapeptide-1 is a synthetic hexapeptide widely used in anti-wrinkle products. Its mechanism of action mainly involves inhibiting the release of neurotransmitters and reducing muscle contraction intensity, thereby achieving a "botox-like" effect similar to Botox and helping to reduce the formation of dynamic wrinkles. Palmitoyl pentapeptide-4, on the other hand, is a collagen-stimulating peptide that effectively stimulates dermal fibroblasts to synthesize type I and type III collagen, enhancing skin structural support, improving skin laxity, and exhibiting good anti-wrinkle and firming effects. Furthermore, plant extracts, due to their natural and gentle nature and various biological activities (such as antioxidant, anti-inflammatory, moisturizing, and soothing properties), are widely used in skincare formulations to help improve the overall efficacy and safety of products. For example, green tea extract, centella asiatica extract, and polygonum cuspidatum root extract have been proven to have strong free radical scavenging capabilities, which can synergistically work with peptide components to resist skin damage caused by photoaging and oxidative stress. Therefore, providing a composition combining peptide compounds and extracts has significant application value in improving the overall effect of products.

[0004] Furthermore, micro and nanomaterials, due to their unique physicochemical properties and excellent biocompatibility, show broad application prospects in drug delivery, targeted therapy, and high-end skincare products. Among them, submicron-sized particles (typically ranging from 100 nm to 1 μm in size) possess excellent skin penetration capabilities due to their moderate particle size, and can effectively load active ingredients to achieve sustained-release effects, thereby significantly improving the stability and efficacy of skincare products. Therefore, further developing a submicron spherical composition and applying it to the preparation of skincare products can enhance the overall performance and market competitiveness of the products. Summary of the Invention

[0005] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides a composition, a submicron gold sphere composition and its application in the preparation of skin care products.

[0006] The technical solution of the present invention is as follows: The present invention first provides a composition comprising amino acid peptides and plant extracts; The amino acid polypeptide is selected from one or more of acetyl hexapeptide-1, acetyl hexapeptide-8, palmitoyl pentapeptide-4, and snake venom-like peptides; The plant extracts mentioned are selected from Chlorella fermentation products, Porphyra fermentation products, Centella asiatica extract and / or Ganoderma lucidum extract.

[0007] This invention provides a novel composition that combines amino acid peptides and plant extracts, and also has anti-inflammatory effects.

[0008] Preferably, the weight ratio of the amino acid polypeptide to the plant extract is 1:1 to 10.

[0009] Preferably, the weight ratio of the amino acid polypeptide to the plant extract is 1:5.

[0010] Preferably, the amino acid polypeptide is selected from a combination of acetyl hexapeptide-1 and palmitoyl pentapeptide-4.

[0011] Preferably, the weight ratio of acetyl hexapeptide-1 to palmitoyl pentapeptide-4 is 1~3:1~3.

[0012] Most preferably, the weight ratio of acetyl hexapeptide-1 to palmitoyl pentapeptide-4 is 1:1.

[0013] Preferably, the plant extract is selected from Ganoderma lucidum extract.

[0014] Preferably, the Ganoderma lucidum extract is prepared by the following method: Ganoderma lucidum powder is obtained by crushing the Ganoderma lucidum into powder and passing it through an 80-200 mesh sieve. Ganoderma lucidum powder was extracted with an organic solvent. After extraction, the extract was separated, concentrated and dried to obtain Ganoderma lucidum organic solvent extract. The Ganoderma lucidum organic solvent extract is then taken to obtain the Ganoderma lucidum extract.

[0015] Preferably, the ratio of Ganoderma lucidum powder to organic solvent is 1 kg: 8-15 L.

[0016] The most preferred ratio of Ganoderma lucidum powder to organic solvent is 1 kg: 12 L.

[0017] Preferably, the organic solvent is selected from ethyl acetate or cyclohexane.

[0018] Preferably, the organic solvent is selected from a combination of ethyl acetate and cyclohexane.

[0019] The volume ratio of ethyl acetate to cyclohexane is 1~3:1.

[0020] Most preferably, the volume ratio of ethyl acetate to cyclohexane is 2:1.

[0021] Further research by the inventors revealed that the Ganoderma lucidum extract obtained by adding an organic solvent extract using a combination of ethyl acetate and cyclohexane to the composition described in this invention exhibits significantly higher anti-inflammatory effects than the Ganoderma lucidum extract obtained by adding only ethyl acetate or cyclohexane as the organic solvent extract. Furthermore, the Ganoderma lucidum extract obtained by using an organic solvent extract using a combination of ethyl acetate and cyclohexane can synergistically enhance the anti-inflammatory effects of the composition.

[0022] Preferably, the method for preparing the Ganoderma lucidum extract further includes the following steps: The Ganoderma lucidum organic solvent extract was loaded onto a silica gel column and eluted with a mixed organic solvent of chloroform and methanol in a volume ratio of 100:10:13 (3-5 column volumes) to remove impurities. Then, it was eluted again with a mixed organic solvent of chloroform and methanol in a volume ratio of 100:26:28 (3-5 column volumes). The eluent obtained from the elution with the mixed organic solvent of chloroform and methanol in a volume ratio of 100:26:28 was collected, concentrated, and dried to obtain the Ganoderma lucidum extract.

[0023] Most preferably, the method for preparing the Ganoderma lucidum extract further includes the following steps: The Ganoderma lucidum organic solvent extract was loaded onto a silica gel column and eluted with a mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:12 (4 column volumes) to remove impurities. Then, it was eluted again with a mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:27 (4 column volumes). The eluent obtained from the elution was collected, concentrated, and dried to obtain the Ganoderma lucidum extract.

[0024] In further research, the inventors discovered that adding Ganoderma lucidum extract prepared under the silica gel column elution conditions described above to the composition of this invention significantly enhanced its anti-inflammatory effect.

[0025] The inventors also discovered in their research that the silica gel column elution conditions of the present invention are crucial; the anti-inflammatory effect can only be significantly enhanced by adding Ganoderma lucidum extract prepared under the silica gel column elution conditions described above in the composition of the present invention; however, the anti-inflammatory effect cannot be significantly enhanced by adding Ganoderma lucidum extract prepared under other silica gel column elution conditions.

[0026] Preferably, the organic solvent is acetone.

[0027] The present invention also provides a submicron gold sphere composition, which is prepared by the following method: The above composition is added to water and dispersed evenly. Then, submicron gold spheres are added, and the composition is grafted onto the surface of the submicron gold spheres by a self-assembly grafting method to form a micro spherical structure. The micro spherical structure is then separated by centrifugation and dried to obtain the submicron gold sphere composition.

[0028] Most preferably, the ratio of the composition, water, and submicron gold spheres is 1~2g:20~40mL:3~8g.

[0029] Most preferably, the ratio of the composition, water, and submicron gold spheres is 1g:20mL:5g.

[0030] The submicron gold spheres are spherical gold powders with a particle size in the submicron range.

[0031] Application of the above composition or submicron gold sphere composition in the preparation of skin care products.

[0032] Preferably, the skincare product is a skincare product with anti-inflammatory properties.

[0033] Beneficial effects: This invention provides a novel composition that combines amino acid peptides and plant extracts; it not only possesses the relevant effects of amino acid peptides but also has anti-inflammatory properties; therefore, using the composition of this invention as an active ingredient in the preparation of skincare products has significant application value.

[0034] Furthermore, based on the above composition, the present invention also employs submicron encapsulation technology to encapsulate the above composition, providing a submicron gold sphere composition; this submicron gold sphere composition not only has good skin penetration ability, but also effectively loads active ingredients and achieves sustained release, thereby significantly improving the stability and efficacy of skin care products. Detailed Implementation

[0035] The present invention will be further explained below with reference to specific embodiments, but the embodiments do not limit the present invention in any way.

[0036] Example 1 Preparation of the composition The composition is obtained by mixing amino acid peptides and plant extracts at a weight ratio of 1:5. The amino acid polypeptide is composed of acetyl hexapeptide-1 and palmitoyl pentapeptide-4 in a weight ratio of 1:1. The plant extract mentioned is Ganoderma lucidum extract; The Ganoderma lucidum extract was prepared by the following method: (1) Ganoderma lucidum powder is obtained by crushing Ganoderma lucidum into powder and passing it through a 100-mesh sieve. (2) Heat Ganoderma lucidum powder to boiling with organic solvent and reflux for 70 min. After extraction, separate the extract and concentrate and dry the extract to obtain Ganoderma lucidum organic solvent extract. Take the Ganoderma lucidum organic solvent extract to obtain the Ganoderma lucidum extract. The ratio of Ganoderma lucidum powder to organic solvent is 1 kg: 12 L. The organic solvent is ethyl acetate.

[0037] Example 2 Preparation of the composition The composition is obtained by mixing amino acid peptides and plant extracts at a weight ratio of 1:5. The amino acid polypeptide is composed of acetyl hexapeptide-1 and palmitoyl pentapeptide-4 in a weight ratio of 1:1. The plant extract mentioned is Ganoderma lucidum extract; The Ganoderma lucidum extract was prepared by the following method: (1) Ganoderma lucidum powder is obtained by crushing Ganoderma lucidum into powder and passing it through a 100-mesh sieve. (2) Heat Ganoderma lucidum powder to boiling with organic solvent and reflux for 70 min. After extraction, separate the extract and concentrate and dry the extract to obtain Ganoderma lucidum organic solvent extract. Take the Ganoderma lucidum organic solvent extract to obtain the Ganoderma lucidum extract. The ratio of Ganoderma lucidum powder to organic solvent is 1 kg: 12 L. The organic solvent is cyclohexane.

[0038] Example 3 Preparation of the composition The composition is obtained by mixing amino acid peptides and plant extracts at a weight ratio of 1:5. The amino acid polypeptide is composed of acetyl hexapeptide-1 and palmitoyl pentapeptide-4 in a weight ratio of 1:1. The plant extract mentioned is Ganoderma lucidum extract; The Ganoderma lucidum extract was prepared by the following method: (1) Ganoderma lucidum powder is obtained by crushing Ganoderma lucidum into powder and passing it through a 100-mesh sieve. (2) Heat Ganoderma lucidum powder to boiling with organic solvent and reflux for 70 min. After extraction, separate the extract and concentrate and dry the extract to obtain Ganoderma lucidum organic solvent extract. Take the Ganoderma lucidum organic solvent extract to obtain the Ganoderma lucidum extract. The ratio of Ganoderma lucidum powder to organic solvent is 1 kg: 12 L. The organic solvent is composed of ethyl acetate and cyclohexane in a volume ratio of 2:1.

[0039] Example 4 Preparation of the composition The composition is obtained by mixing amino acid peptides and plant extracts at a weight ratio of 1:5. The amino acid polypeptide is composed of acetyl hexapeptide-1 and palmitoyl pentapeptide-4 in a weight ratio of 1:1. The plant extract mentioned is Ganoderma lucidum extract; The Ganoderma lucidum extract was prepared by the following method: (1) Ganoderma lucidum powder is obtained by crushing Ganoderma lucidum into powder and passing it through a 100-mesh sieve. (2) Ganoderma lucidum powder is heated to boiling with organic solvent and then refluxed for 70 min. After extraction, the extract is separated and concentrated and dried to obtain Ganoderma lucidum organic solvent extract. The ratio of Ganoderma lucidum powder to organic solvent is 1 kg: 12 L. The organic solvent is composed of ethyl acetate and cyclohexane in a volume ratio of 2:1. (3) Load the Ganoderma lucidum organic solvent extract onto a silica gel column (the silica gel column is filled with 200-300 mesh silica gel at a volume ratio of 30 times that of the Ganoderma lucidum organic solvent extract). First, use a mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:12 (4 column volumes) to remove impurities. Then, use a mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:27 (4 column volumes) to elute again. Collect the eluent eluted by the mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:27, concentrate and dry it to obtain the Ganoderma lucidum extract.

[0040] Comparative Example 1: Preparation of the Composition The composition is obtained by mixing amino acid peptides and plant extracts at a weight ratio of 1:5. The amino acid polypeptide is composed of acetyl hexapeptide-1 and palmitoyl pentapeptide-4 in a weight ratio of 1:1. The plant extract mentioned is Ganoderma lucidum extract; The Ganoderma lucidum extract was prepared by the following method: (1) Ganoderma lucidum powder is obtained by crushing Ganoderma lucidum into powder and passing it through a 100-mesh sieve. (2) Ganoderma lucidum powder is heated to boiling with organic solvent and then refluxed for 70 min. After extraction, the extract is separated and concentrated and dried to obtain Ganoderma lucidum organic solvent extract. The ratio of Ganoderma lucidum powder to organic solvent is 1 kg: 12 L. The organic solvent is composed of ethyl acetate and cyclohexane in a volume ratio of 2:1. (3) Load the Ganoderma lucidum organic solvent extract onto a silica gel column (the silica gel column is filled with 200-300 mesh silica gel at a volume ratio of 30 times that of the Ganoderma lucidum organic solvent extract). First, use a mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:1 to remove impurities, which is 4 times the column volume. Then, use a mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:10 to remove impurities. Collect the eluent eluted by the mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:10, concentrate and dry it to obtain the Ganoderma lucidum extract.

[0041] Comparative Example 2: Preparation of the Composition The composition is obtained by mixing amino acid peptides and plant extracts at a weight ratio of 1:5. The amino acid polypeptide is composed of acetyl hexapeptide-1 and palmitoyl pentapeptide-4 in a weight ratio of 1:1. The plant extract mentioned is Ganoderma lucidum extract; The Ganoderma lucidum extract was prepared by the following method: (1) Ganoderma lucidum powder is obtained by crushing Ganoderma lucidum into powder and passing it through a 100-mesh sieve. (2) Ganoderma lucidum powder is heated to boiling with organic solvent and then refluxed for 70 min. After extraction, the extract is separated and concentrated and dried to obtain Ganoderma lucidum organic solvent extract. The ratio of Ganoderma lucidum powder to organic solvent is 1 kg: 12 L. The organic solvent is composed of ethyl acetate and cyclohexane in a volume ratio of 2:1. (3) Load the Ganoderma lucidum organic solvent extract onto a silica gel column (the silica gel column is filled with 200-300 mesh silica gel at a volume ratio of 30 times that of the Ganoderma lucidum organic solvent extract). First, use a mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:25 (4 column volumes) to remove impurities. Then, use a mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:50 (4 column volumes) to elute again. Collect the eluent eluted by the mixed organic solvent consisting of chloroform and methanol in a volume ratio of 100:50, concentrate and dry it to obtain the Ganoderma lucidum extract.

[0042] Anti-inflammatory experiment HaCaT cells were added to DMEM medium and cultured in a cell culture incubator at 37°C and 5% CO2 for 24 h. Then, the medium was aspirated, and the experimental and irradiated groups were treated with 8 × 10⁻⁶ cells / mL solution. -5 J / cm 2 Cells were irradiated with ultraviolet light at a distance of 20 cm. After irradiation, the irradiated group was cultured in DMEM medium for 24 h, while the experimental group was cultured in DMEM medium containing 15 μg / mL of the test composition for 24 h. A normal group was also included, but without ultraviolet light irradiation. Each group was repeated three times. After the experiment, the cell culture medium was collected from each group, and the content of the inflammatory factor IL-1β in the cell culture medium was detected using ELISA reagents according to the instructions. The relative content of IL-1β in the experimental group and the model group relative to the blank group was calculated based on the content of IL-1β in the blank group. The results are shown in Table 1. The relative content of IL-1β (%) = (the content of IL-1β in the experimental group or the irradiated group / the content of IL-1β in the blank control group) * 100%.

[0043] Table 1. Anti-inflammatory test results of the compositions of the present invention Test composition Relative content of IL-1β (%) Irradiation group none 338.9±32.1 Experimental group 1 Example 1 Composition 261.4±27.7 Experimental group 2 Example 2 Composition 283.6±28.6 Experimental group 3 Example 3 Composition 225.1±23.7 Experimental group 4 Example 4 Composition 132.2±15.9 Experimental group 5 Comparative Example 1 Composition 181.3±20.2 Experimental group 6 Comparative Example 2 Composition 205.2±24.9 As can be seen from the experimental results in Table 1, the relative content of the inflammatory factor IL-1β in experimental groups 1 and 2 was significantly lower than that in the irradiated group; this indicates that the composition obtained by combining amino acid peptides and Ganoderma lucidum extract prepared by the method described in this invention has anti-inflammatory effects.

[0044] As can be seen from the experimental results in Table 1, the relative content of the inflammatory factor IL-1β in experimental group 3 was significantly lower than that in experimental groups 1 and 2. This indicates that the Ganoderma lucidum extract obtained by adding an organic solvent extract using a combination of ethyl acetate and cyclohexane to the composition described in this invention has a significantly higher anti-inflammatory effect than the Ganoderma lucidum extract obtained by adding only ethyl acetate or cyclohexane as an organic solvent extract. The Ganoderma lucidum extract obtained by using an organic solvent extract using a combination of ethyl acetate and cyclohexane can synergistically enhance the anti-inflammatory effect of the composition.

[0045] As can be seen from the experimental results in Table 1, the relative content of the inflammatory factor IL-1β in experimental group 4 was significantly lower than that in experimental group 3. This indicates that the anti-inflammatory effect of Ganoderma lucidum extract prepared by the silica gel column elution conditions described above was further significantly improved when added to the composition of the present invention.

[0046] As can be seen from the experimental results in Table 1, although the relative content of the inflammatory factor IL-1β in experimental groups 5 and 6 was lower than that in experimental group 3, the decrease was far less significant than that in experimental group 4. This indicates that the silica gel column elution conditions of this invention are crucial; the anti-inflammatory effect can only be significantly enhanced by adding Ganoderma lucidum extract prepared under the silica gel column elution conditions described in this invention to the composition; however, adding Ganoderma lucidum extract prepared under other silica gel column elution conditions does not significantly enhance the anti-inflammatory effect.

[0047] Example 5: Preparation of submicron gold ball composition The composition described in Examples 1, 2, 3 or 4 is added to water and dispersed evenly. Then, submicron gold spheres are added, and the composition is grafted onto the surface of the submicron gold spheres by a self-assembly grafting method to form a micro spherical structure. The micro spherical structure is then separated by centrifugation and dried to obtain the submicron gold sphere composition.

[0048] The composition, water, and submicron gold spheres are used in a ratio of 1g:20mL:5g; the submicron gold spheres are spherical gold powder with a particle size of submicron.

Claims

1. A composition, characterized in that, It contains amino acid peptides and plant extracts; The amino acid polypeptide is selected from one or more of acetyl hexapeptide-1, acetyl hexapeptide-8, palmitoyl pentapeptide-4, and snake venom-like peptides; The plant extracts mentioned are selected from Chlorella fermentation products, Porphyra fermentation products, Centella asiatica extract and / or Ganoderma lucidum extract.

2. The composition according to claim 1, characterized in that, The weight ratio of the amino acid peptides to the plant extracts is 1:1 to 10.

3. The composition according to claim 2, characterized in that, The weight ratio of the amino acid peptides to the plant extracts is 1:

5.

4. The composition according to claim 1, characterized in that, The amino acid polypeptide is selected from a combination of acetyl hexapeptide-1 and palmitoyl pentapeptide-4.

5. The composition according to claim 4, characterized in that, The weight ratio of acetyl hexapeptide-1 to palmitoyl pentapeptide-4 is 1~3:1~3.

6. The composition according to claim 4, characterized in that, The plant extract mentioned is selected from Ganoderma lucidum extract.

7. The composition according to claim 5, characterized in that, The Ganoderma lucidum extract was prepared by the following method: Ganoderma lucidum powder is obtained by crushing the Ganoderma lucidum into powder and passing it through an 80-200 mesh sieve. Ganoderma lucidum powder was extracted with an organic solvent. After extraction, the extract was separated, concentrated and dried to obtain Ganoderma lucidum organic solvent extract. The Ganoderma lucidum organic solvent extract is then taken to obtain the Ganoderma lucidum extract.

8. The composition according to claim 7, characterized in that, The organic solvent is acetone or cyclohexane.

9. A submicron gold sphere composition, characterized in that, The composition is prepared by the following method: the composition according to any one of claims 1 to 8 is added to water and dispersed evenly, then submicron gold spheres are added, and the composition is grafted onto the surface of the submicron gold spheres by a self-assembly grafting method to form a micro spherical structure; then the micro spherical structure is separated by centrifugation and dried to obtain the submicron gold sphere composition.

10. The use of the composition or submicron gold sphere composition according to any one of claims 1 to 9 in the preparation of skin care products.