Makeup removing wet tissue containing papain and preparation method thereof

Abstract

The invention discloses makeup removing wet tissue containing papain and a preparation method of the makeup removing wet tissue. The makeup removing wet tissue containing papain comprises a base material and a liquid composition, wherein the liquid composition comprises the following components in percentage by weight: 0.1 to 1.0 percent of compound enzyme or compound enzyme microcapsules, 2 to 4 percent of sodium cocoyl glutamate, 3 to 10 percent of capric triglyceride, 0.05 to 0.1 percent of centella asiatica extract, 0.02 to 0.05 percent of bisabolol and the balance of water. The invention also provides a preparation method of the makeup removing wet tissue bag, which comprises the following steps: firstly preparing the liquid composition, then soaking the spunlace non-woven fabric in the liquid composition, and then folding, sealing and packaging to obtain the makeup removing wet tissue bag containing papain. Compared with the prior art, the makeup removing wet tissue containing papain has the advantages of being good in makeup removing effect and moisturizing effect, small in skin irritation, capable of adjusting microbial flora on the skin surface, inhibiting growth of harmful bacteria, promoting generation of beneficial bacteria and the like.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of wet wipes, in particular to a papain-containing makeup-removing wet wipe and a preparation method thereof. BACKGROUND

[0002] Most women will wear makeup in their work or life, and generally use cream-based cosmetics including two types of powder cream and liquid cream. Liquid cream used for makeup can be divided into water cream and oil cream. Oil cream is divided into "water-in-oil type" and "oil-in-water type" according to the amount of oil. For the above-mentioned makeup products, the most common makeup-removing oil and makeup-removing milk on the market are mostly in cans, which are inconvenient to carry when traveling or going on business trips. When traveling or going on business trips, people generally choose light makeup and skin care products, such as small samples. In addition to small samples, a very convenient makeup-removing product is makeup-removing wet wipes. Makeup-removing wet wipes are disposable cleaning and sanitary products that assist in makeup removal and have the basic function of cleaning the skin. Non-woven fabric is used as a carrier, and a cleaning liquid containing makeup-removing ingredients is added to achieve the purpose of makeup removal by wiping. Makeup-removing wet wipes are very suitable for some emergency situations without clean water, such as during travel or camping.

[0003] The makeup-removing wet wipes on the market generally have the problems of incomplete cleaning of waterproof makeup, uncomfortable skin after cleaning, residual makeup, poor moisturizing function, etc. Generally, they only have the basic functions of cleaning and moisturizing the skin, not to mention the functions of moisturizing, regulating skin balance, and other skin care functions. Therefore, it is necessary to develop makeup-removing wet wipes with better functions.

[0004] CN114259451A discloses a wet wipe convenient for removing makeup and a preparation method thereof, comprising dust-free paper and makeup remover; the makeup remover comprises the following raw material components: cetyl pyridinium chloride 0.03-0.05%, ethylhexyl glycerin 0.05-0.07%, phenoxyethanol 0.4-0.8%, aloe extract 0.5-0.9%, glycerin 1-3%, PEG-7 glyceryl cocoate 1.5-2.1%, propylene glycol 0.5-0.7%, vitamin E acetate 0.1-0.2%, polysorbate-20 0.2-0.4%, pro-vitamin B5 0.05-0.08%, fullerene 0.05-0.08% and Japanese late cherry blossom extract 0.05-0.09%, and the balance is deionized water, which improves the storage time of the makeup removal wet wipe. However, the main makeup removal component of the invention is a surfactant, which may cause damage to the skin barrier. CN110141547A discloses an anti-allergic multi-effect makeup removal wipe, comprising a paper towel layer, a packaging layer wrapping the paper towel layer and a makeup removal liquid soaking the paper towel layer, the makeup removal liquid comprising plant extracts, humectants, surfactants, vegetable oils, vitamins, emulsifiers and the balance of water, which improves the anti-allergic performance by adding plant extracts. However, the invention may have the disadvantage of low makeup removal cleaning ability. SUMMARY

[0005] In view of the above-mentioned defects of the prior art, the purpose of the present application is to provide a papain-containing makeup removal wet wipe, which has good makeup removal effect and good moisturizing effect, has small skin irritation, can regulate the skin surface microbial flora, inhibit the growth of harmful bacteria and promote the generation of beneficial bacteria.

[0006] To achieve the above-mentioned purpose, the present application provides a papain-containing makeup removal wet wipe, comprising a substrate and a liquid composition, the liquid composition comprising the following components by weight percentage: 0.1-1.0% complex enzyme or complex enzyme microcapsule, 2-4% sodium cocoyl glutamate, 3-10% capric acid triglyceride, 0.05-0.1% gotu kola extract, 0.02-0.05% farnesol, and the balance is water.

[0007] Preferably, the substrate is a spunlace non-woven fabric.

[0008] Preferably, the pH value of the liquid composition is 4.5-6.5.

[0009] Preferably, the mass ratio of the substrate to the liquid composition is 1:2-4.

[0010] Preferably, the complex enzyme is compounded by papain and bromelain at a mass ratio of 1-5:1-5.

[0011] Preferably, the preparation method of the complex enzyme microcapsule comprises the following steps, by mass fraction: Step 1, 1-5 parts of fish skin gelatin is swelled in 20-40 parts of water for 1-3 hours, then stirred at 30-50℃ for 5-15 minutes, 0.1-1 parts of modified hyaluronic acid and 0.1-0.5 parts of complex enzyme are added, stirred for 20-40 minutes, to obtain fish skin gelatin mixture; Step 2, 0.5-1.5 parts of polyglycerol ricinoleate is added to 10-30 parts of soybean oil, stirred for 5-15 minutes, to obtain oil phase for standby; Step 3, 1-5 parts of fish skin gelatin mixture obtained in step 1 is added to the oil phase obtained in step 2 and mixed uniformly, then 0.1-1.0 parts of tannic acid is added, stirred at 30-50℃ for 5-7 hours, centrifuged for 5-15 minutes, the precipitate is collected, washed with water, and freeze-dried to obtain complex enzyme microcapsules.

[0012] As a further illustration of the present application, fish skin gelatin can be used as a wall material, modified hyaluronic acid and gelatin chains are intertwined to enhance strength, and tannic acid is further crosslinked to form a microcapsule structure. It can protect the complex enzyme, and the modified hyaluronic acid can improve the makeup removal effect, moisturizing effect, and also regulate the skin surface microbial flora, inhibit the growth of harmful bacteria, and promote the growth of beneficial bacteria.

[0013] Preferably, the preparation method of the modified hyaluronic acid comprises the following steps, by mass fraction: 50-150 parts of hyaluronic acid and 15000-25000 parts of water are mixed uniformly, sterilized, cooled, inoculated with 0.1-0.5wt% complex bacterial solution, and fermented at 40-45℃ for 8-12 hours; centrifuged for 20-40 minutes, the supernatant is collected, sterilized to obtain hyaluronic acid fermentation liquor; 1-5 parts of modifier and 250-350 parts of water are mixed uniformly, then 10-50 parts of hyaluronic acid fermentation liquor is added and mixed uniformly, and freeze-dried to obtain modified hyaluronic acid.

[0014] Preferably, the complex bacterial solution is obtained by mixing Lactobacillus rhamnosus and Lactobacillus plantarum, and the viable bacterial count of the bacterial solution is 0.5-1.5×10 9 CFU / mL.

[0015] Further preferably, the Lactobacillus rhamnosus and Lactobacillus plantarum are compounded according to a viable bacterial count ratio of 1-5:1-5.

[0016] Preferably, the modifier is at least one of xanthan gum, beta-glucan, and konjac glucomannan.

[0017] As a further explanation of the present invention, the molecular size of large-molecule hyaluronic acid can be reduced by Lactobacillus rhamnosus and Lactobacillus plantarum, and it also has a variety of biological activities such as deep repair, soothing and anti-inflammatory, and promoting collagen production. Furthermore, through the reaction with the modifier, it can further enhance the effects of makeup removal, moisturizing, regulating the skin surface microbial flora, inhibiting the growth of harmful bacteria, and promoting the generation of beneficial bacteria.

[0018] This invention also provides a method for preparing makeup remover wipes containing papain, comprising the following steps: Step 1: Weigh out the compound enzyme or compound enzyme microcapsules, sodium cocoyl glutamate, triglyceride decanoate, Centella asiatica extract, bisabolol, and water, then mix the substances to prepare a liquid composition. Step 2: Immerse the sterilized and dried substrate in the above liquid composition, then fold, seal and package to obtain makeup remover wipes containing papain.

[0019] The beneficial effects of this invention are: 1. This invention utilizes the interaction between various substances through reasonable proportioning and optimizes the preparation process to obtain makeup remover wipes containing papain. The makeup remover wipes containing papain obtained by this invention have the advantages of good makeup removal effect, good moisturizing effect, low skin irritation, ability to regulate the microbial flora on the skin surface, inhibit the growth of harmful bacteria, and promote the generation of beneficial bacteria.

[0020] 2. Compared with the prior art, the present invention, through the synergistic effect of compound enzymes and modified hyaluronic acid, can protect enzyme activity, improve makeup removal effect, moisturize effect, reduce skin irritation, regulate the skin surface microbial flora, inhibit the growth of harmful bacteria, and promote the generation of beneficial bacteria. Detailed Implementation

[0021] The parameters for using specific chemical substances, and their sources.

[0022] Papain, 200,000 U / g; Bromelain, 100,000 U / g; Preparation method of Centella asiatica extract: 100g of dried Centella asiatica was pulverized and passed through a 100-mesh sieve. 1000g of water was added, and the mixture was extracted twice under reflux at 100℃, each extraction lasting 1.5 hours. The filtrates were combined and concentrated to a density of 1.20g / cm³ at 25℃. 3 This yields Centella Asiatica extract.

[0023] Lactobacillus rhamnosus, a commercially available product, strain number: CICC 6137, purchased from the China Industrial Microbial Culture Collection Center; Lactobacillus plantarum, a commercially available product, strain number: CICC 20331, purchased from the China Industrial Microbial Culture Collection Center; Fish gelatin, brand: Dongbao Biology, from Baotou Dongbao Biotechnology Co., Ltd.; Xanthan gum, brand: Fentai, model: FT11, from Shandong Fentai Biological Technology Co., Ltd.; Konjac glucomannan, product number: S30903, specification: viscosity ≥ 15000 mpa.s, from Shanghai Yuan Ye Biological Technology Co., Ltd.; Beta-glucan, product number: S24487, from Shanghai Yuan Ye Biological Technology Co., Ltd.; Polyglycerol ricinoleate, item number: 0002, from Wuhan Prolife Biological Technology Co., Ltd.

[0024] Example 1 A preparation method of a papain-containing makeup remover wet wipe, comprising the following steps: Step one, weigh 0.5% complex enzyme, 3% sodium cocoyl glutamate, 5% capric acid triglyceride, 0.08% gota kola extract, 0.03% bisabalol, and the rest is water, then mix the substances to obtain a liquid composition, wherein the pH value of the liquid composition is 5.0; Step two, soak the sterilized and disinfected dry spunlace non-woven fabric in the above-mentioned liquid composition, the mass ratio of spunlace non-woven fabric to liquid composition is 1:3, then fold, seal and package, to obtain a papain-containing makeup remover wet wipe.

[0025] The complex enzyme is a mixture of papain and bromelain in a mass ratio of 3:2.

[0026] Example 2 A preparation method of a papain-containing makeup remover wet wipe, comprising the following steps: Step one, weigh 0.5% complex enzyme microcapsule, 3% sodium cocoyl glutamate, 5% capric acid triglyceride, 0.08% gota kola extract, 0.03% bisabalol, and the rest is water, then mix the substances to obtain a liquid composition, wherein the pH value of the liquid composition is 5.0; Step two, soak the sterilized and disinfected dry spunlace non-woven fabric in the above-mentioned liquid composition, the mass ratio of spunlace non-woven fabric to liquid composition is 1:3, then fold, seal and package, to obtain a papain-containing makeup remover wet wipe.

[0027] The preparation method of the complex enzyme microcapsule comprises the following steps: Step 1, swell 3g fish gelatin in 30g water for 2h, then stir at 40℃ for 10min, add 0.5g modified hyaluronic acid and 0.3g complex enzyme, stir for 30min to obtain a fish gelatin mixture; Step 2, 1g polyglycerol ricinoleate was added to 20g soybean oil, stirred for 10min, and the oil phase was prepared for standby; Step 3, 3g fish skin gelatin mixed solution obtained in step 1 was added to the oil phase obtained in step 2 and mixed uniformly, then 0.6g tannic acid was added, stirred at 40℃ for 6h, centrifuged at 4000rpm for 10min, the precipitate was collected, washed with water, and freeze-dried to obtain the composite enzyme microcapsule.

[0028] The composite enzyme is papain and bromelain compounded according to a mass ratio of 3:2.

[0029] The preparation method of the modified hyaluronic acid comprises the following steps: 100g hyaluronic acid and 20000g water were mixed uniformly, sterilized, cooled, inoculated with 0.3wt% composite bacterial solution, and fermented at 43℃ for 10h; centrifuged at 4800rpm for 30min, the supernatant was collected, sterilized to obtain a hyaluronic acid fermentation liquor; 3g xanthan gum and 300g water were mixed uniformly, then 30g of the hyaluronic acid fermentation liquor was added and mixed uniformly, and freeze-dried to obtain the modified hyaluronic acid.

[0030] The composite bacterial solution is selected from Lactobacillus rhamnosus and Lactobacillus plantarum compounded according to a mass ratio of 2:3, and the viable bacterial count of the bacterial solution is 1.0×10 9 CFU / mL.

[0031] Example 3 A preparation method of a makeup remover wet wipe containing papain, which is different from example 2 only in that the preparation method of the composite enzyme microcapsule is different.

[0032] The preparation method of the composite enzyme microcapsule comprises the following steps: Step 1, 3g fish skin gelatin was swelled in 30g water for 2h, then stirred at 40℃ for 10min, 0.5g modified hyaluronic acid and 0.3g composite enzyme were added, and stirred for 30min to obtain a fish skin gelatin mixed solution; Step 2, 1g polyglycerol ricinoleate was added to 20g soybean oil, stirred for 10min, and the oil phase was prepared for standby; Step 3, 3g fish skin gelatin mixed solution obtained in step 1 was added to the oil phase obtained in step 2 and mixed uniformly, then 0.6g tannic acid was added, stirred at 40℃ for 6h, centrifuged at 4000rpm for 10min, the precipitate was collected, washed with water, and freeze-dried to obtain the composite enzyme microcapsule.

[0033] The composite enzyme is papain and bromelain compounded according to a mass ratio of 3:2.

[0034] The preparation method of the modified hyaluronic acid comprises the following steps: uniformly mixing 100 g of hyaluronic acid and 20,000 g of water, sterilizing, cooling, inoculating 0.3 wt% of the complex bacterial solution, and fermenting at 43°C for 10 h; centrifuging at 4,800 rpm for 30 min, collecting the supernatant, sterilizing to obtain a hyaluronic acid fermentation liquor; uniformly mixing 3 g of β-glucan and 300 g of water, then adding 30 g of the hyaluronic acid fermentation liquor, and uniformly mixing, and freeze-drying to obtain the modified hyaluronic acid.

[0035] The complex bacterial solution is prepared by compounding Lactobacillus rhamnosus and Lactobacillus plantarum at a mass ratio of 2:3, and the viable bacterial count of the bacterial solution is 1.0×10 9 CFU / mL.

[0036] Example 4 A preparation method of a papain-containing makeup-removing wet wipe, which is different from that of Example 2 only in that the preparation method of the complex enzyme microcapsule is different.

[0037] The preparation method of the complex enzyme microcapsule comprises the following steps: Step 1, swelling 3 g of fish skin gelatin in 30 g of water for 2 h, then stirring at 40°C for 10 min, adding 0.5 g of modified hyaluronic acid and 0.3 g of complex enzyme, and stirring for 30 min to obtain a fish skin gelatin mixture; Step 2, adding 1 g of polyglycerol ricinoleate to 20 g of soybean oil, and stirring for 10 min to obtain an oil phase for standby; Step 3, uniformly mixing 3 g of the fish skin gelatin mixture obtained in Step 1 into the oil phase obtained in Step 2, then adding 0.6 g of tannic acid, stirring at 40°C for 6 h, centrifuging at 4,000 rpm for 10 min, collecting the precipitate, washing with water, and freeze-drying to obtain the complex enzyme microcapsule.

[0038] The complex enzyme is prepared by compounding papain and bromelain at a mass ratio of 3:2.

[0039] The preparation method of the modified hyaluronic acid comprises the following steps: uniformly mixing 100 g of hyaluronic acid and 20,000 g of water, sterilizing, cooling, inoculating 0.3 wt% of the complex bacterial solution, and fermenting at 43°C for 10 h; centrifuging at 4,800 rpm for 30 min, collecting the supernatant, sterilizing to obtain a hyaluronic acid fermentation liquor; uniformly mixing 3 g of β-glucan and 300 g of water, then adding 30 g of the hyaluronic acid fermentation liquor, and uniformly mixing, and freeze-drying to obtain the modified hyaluronic acid.

[0040] The complex bacterial solution is prepared by compounding Lactobacillus rhamnosus and Lactobacillus plantarum at a viable bacterial count ratio of 2:3, and the viable bacterial count of the bacterial solution is 1.0×10 9 CFU / mL.

[0041] Comparative Example 1 A preparation method of a papain-containing makeup-removing wet wipe, which is only different from that of Example 2 in that the preparation method of the composite enzyme microcapsule is different.

[0042] The preparation method of the composite enzyme microcapsule comprises the following steps: Step 1, 3 g of fish skin gelatin is swelled in 30 g of water for 2 h, then stirred at 40 ℃ for 10 min, 0.5 g of modified hyaluronic acid and 0.3 g of composite enzyme are added, and stirred for 30 min to obtain a fish skin gelatin mixture; Step 2, 1 g of polyglycerol ricinoleate is added to 20 g of soybean oil, and stirred for 10 min to obtain an oil phase for standby; Step 3, 3 g of the fish skin gelatin mixture obtained in Step 1 is added to the oil phase obtained in Step 2 and mixed uniformly, then 0.6 g of tannic acid is added, stirred at 40 ℃ for 6 h, centrifuged at 4000 rpm for 10 min, the precipitate is collected, washed with water, and freeze-dried to obtain the composite enzyme microcapsule.

[0043] The composite enzyme is compounded by papain and bromelain at a mass ratio of 3:2.

[0044] The preparation method of the modified hyaluronic acid comprises the following steps: 100 g of hyaluronic acid and 20000 g of water are mixed uniformly to obtain a hyaluronic acid aqueous solution; 3 g of konjac glucomannan and 300 g of water are mixed uniformly, then 30 g of the hyaluronic acid aqueous solution is added and mixed uniformly, and freeze-dried to obtain the modified hyaluronic acid.

[0045] Comparative Example 2 A preparation method of a papain-containing makeup-removing wet wipe, which is only different from that of Example 2 in that the preparation method of the composite enzyme microcapsule is different.

[0046] The preparation method of the composite enzyme microcapsule comprises the following steps: Step 1, 3 g of fish skin gelatin is swelled in 30 g of water for 2 h, then stirred at 40 ℃ for 10 min, 0.5 g of modified hyaluronic acid and 0.3 g of composite enzyme are added, and stirred for 30 min to obtain a fish skin gelatin mixture; Step 2, 1 g of polyglycerol ricinoleate is added to 20 g of soybean oil, and stirred for 10 min to obtain an oil phase for standby; Step 3, 3 g of the fish skin gelatin mixture obtained in Step 1 is added to the oil phase obtained in Step 2 and mixed uniformly, then 0.6 g of tannic acid is added, stirred at 40 ℃ for 6 h, centrifuged at 4000 rpm for 10 min, the precipitate is collected, washed with water, and freeze-dried to obtain the composite enzyme microcapsule.

[0047] The composite enzyme is compounded by papain and bromelain at a mass ratio of 3:2.

[0048] Test Example 1 Human patch test and safety test Human patch test Select 60 test subjects who meet the subject criteria, and randomly divide them into 6 groups, 10 people in each group, to perform patch tests on the cosmetic makeup remover compositions provided in Examples 1-4 and Comparative Examples 1-2: Put the makeup remover products in the test subjects into the chambers of the patch tester, with an amount of about 0.020-0.025 mL, and do not put any substance into the control chambers; apply the patch tester with the test substance to the back or the curved side of the forearm of the test subject with a low-sensitization adhesive tape, and press it evenly onto the skin with the palm for 24 h; observe the skin reaction after 30 min and 24 h after removing the patch tester with the test substance, and record the observation results, with the grading standards shown in Table 1, and the test results shown in Table 2.

[0049] Table 1 Grading standards for skin patch test

[0050] Table 2 Test results of skin patch test

[0051] The above results show that the makeup remover wipes prepared in the present application do not irritate the skin.

[0052] Test Example 2 Repeated skin irritation and acute eye irritation test The samples of Examples 1-4 and Comparative Examples 1-2 were tested for repeated skin irritation and acute eye irritation, and the results are shown in Table 3.

[0053] Chicken embryo chorioallantoic membrane experiment Examples 1-4, Comparative Examples 1-2 and commercially available product A were subjected to chicken embryo chorioallantoic membrane experiments to compare safety, and the liquid components of Examples 1-4 and Comparative Examples 1-2 were taken, the test samples were shaken before sampling, and the data collection was performed by endpoint evaluation method (ES), and the results are shown in Table 3.

[0054] Commercially available product A adopts Zhejiang Gaoqing Network Preparation No. 2021007496, and the formula is: water, isododecane, cyclopentasiloxane, butylene glycol, PEG-6 caprylic / capric glycerides, 1,2-hexanediol, cyclohexasiloxane, panthenol, PEG-40 hydrogenated castor oil, phenoxyethanol, chlorphenesin, disodium EDTA, allantoin, polyaminopropyl biguanide, cetrimonium bromide, octoxyglycerin, camellia japonica seed oil, lonicera japonica flower extract, camellia japonica flower extract, hydrolyzed silk, and ethylhexylglycerin.

[0055] Table 3 Safety test results

[0056] From the comparison of Examples 1-4, Comparative Examples 1-2 and Commercial Product A, it can be found that the safety performance of Examples 1-4 and Comparative Examples 1-2 is higher than that of Commercial Product A, which may be due to the fact that Examples 1-4 and Comparative Examples 1-2 use a combination of biological enzymatic hydrolysis and surfactants, which is more secure.

[0057] Among them, the safety performance of Example 4 is the highest, and the reason may be that the modifier in Example 4 is konjac glucomannan, which has a long molecular chain and no side chain obstacle, and the crosslinking density of the gelatin molecular chain and tannic acid is high, forming a microcapsule structure that can better protect the complex enzyme, thereby reducing the ES score. The modifier of Example 2 is xanthan gum, which has a straight chain backbone and a three-sugar side chain, and the side chain has a large steric hindrance, which hinders the close arrangement of the molecular chain, and the protection effect of the microcapsule structure formed is lower than that of Example 4. The modifier of Example 3 is β-glucan, which has a linear helical structure, and the chain is relatively regular, but the interchain mainly depends on hydrogen bond, and the combination ability with gelatin and tannic acid is lower than that of konjac glucomannan in Example 4, and the effect is lower than that of Example 4.

[0058] Test Example 3 Makeup removal cleaning test Select 60 test persons who meet the subject criteria, and randomly divide them into 6 groups, 10 persons in each group, to test the makeup remover wipes provided by Examples 1-4 and Comparative Examples 1-2: take commercially available makeup (including foundation, eye shadow, lipstick, sunscreen cream) products and apply them to the forearm (1.25 mg / square centimeter), wait for 0.5 h, and then apply the makeup remover wipes provided by Examples 1-4 and Comparative Examples 1-2 to the part where the makeup is applied, take one piece of makeup remover wipe for each test object and wipe for 25 s, and then wash it off with water; test the skin brightness value (L) of the test area before makeup, after makeup, and after makeup removal, respectively, and calculate the makeup removal ability S value (%) by the skin brightness value (L), and the calculation formula is as follows: Makeup removal ability S value (%) = (L 卸妆后- L 上妆后 ) / (L 上妆前- L 上妆后 ) x 100% The test results are averaged, and the test results are shown in Table 4.

[0059] Table 4 Makeup removal cleaning test results

[0060] The makeup removal cleaning ability of Example 4 is the strongest through comparison of Examples 1-4 and Comparative Examples 1-2, and the reason is that the compact microcapsule wall formed by konjac glucomannan can most effectively isolate the potential influence of water, air and other ingredients in the formula on the enzyme, so that the enzyme maintains the highest activity when reaching the skin, and can provide a sustained and stable enzyme concentration under the physical pressure of wiping, and the konjac glucomannan itself has good hydrophilicity and oil absorption, which can assist in adsorbing and dissolving the dissolved makeup particles, and its excellent moisturizing property softens the stratum corneum, which is more conducive to the penetration and enzymatic hydrolysis of makeup.

[0061] Test Example 4 Skin moisture condition test and skin microecology test 60 test persons meeting the subject criteria were selected and randomly divided into 6 groups, 10 persons in each group, and the makeup remover wipes provided by Examples 1-4 and Comparative Examples 1-2 were tested: the test persons used the makeup remover wipes in the morning and at night, the experiment lasted for 28 days, and the measurement of facial biophysical parameters was performed three times, before use, after two weeks of use and after four weeks of use. Facial skin swabs were used for two samplings, before use and after 28 days of use. During the entire experiment, all participants were not allowed to use any skin care products. The amount of transdermal water loss was detected by using an Aquaflux AF200 skin moisture loss tester, and the average value was calculated, and the results are shown in Table 5. Then the biological community on the skin surface was analyzed, and the bacterial flora was identified by 16S rDNA sequencing, and the proportion of beneficial flora (Staphylococcus epidermidis) and harmful flora (P. acnes group, Staphylococcus aureus) was calculated, and the results are shown in Table 6.

[0062] Table 5 Test results of skin moisture condition

[0063] Table 6 Test results of skin microecology

[0064] The effect of Example 4 is the best through comparison of Examples 1-4 and Comparative Examples 1-2, and the reason may be that the amount of water loss using the konjac glucomannan film of Example 4 is the lowest, which can create a more favorable skin environment for beneficial bacteria and less favorable for harmful bacteria, and together with the fermented hyaluronic acid, it forms a virtuous cycle to maintain the skin condition. The difference between Example 4 and Comparative Example 1 is only the fermented hyaluronic acid, and it can be found that the fermented hyaluronic acid can synergistically promote the reduction of water loss, create a more favorable skin environment for beneficial bacteria and less favorable for harmful bacteria, and maintain the skin condition.

Claims

1. A makeup remover wipe containing papain, characterized in that: The product comprises a substrate and a liquid composition, wherein the liquid composition comprises, by weight percentage: 0.1-1.0% complex enzyme or complex enzyme microcapsules, 2-4% sodium cocoyl glutamate, 3-10% triglyceride decanoate, 0.05-0.1% Centella asiatica extract, 0.02-0.05% bisabolol, and the balance being water.

2. The makeup remover wipes containing papain as described in claim 1, characterized in that: The substrate is spunlace nonwoven fabric.

3. The makeup remover wipes containing papain as described in claim 1, characterized in that: The pH value of the liquid composition is 4.5-6.

5.

4. The makeup remover wipes containing papain as described in claim 1, characterized in that: The mass ratio of the substrate to the liquid composition is 1:2-4.

5. The makeup remover wipes containing papain as described in claim 1, characterized in that: The compound enzyme is composed of papain and bromelain in a mass ratio of 1-5:1-5.

6. The makeup remover wipes containing papain as described in claim 1, characterized in that, The preparation method of the composite enzyme microcapsules includes the following steps, by weight: Step 1: Soak 1-5 parts of fish skin gelatin in 20-40 parts of water for 1-3 hours, then stir at 30-50℃ for 5-15 minutes, add 0.1-1 parts of modified hyaluronic acid and 0.1-0.5 parts of compound enzyme, stir for 20-40 minutes to obtain fish skin gelatin mixture; Step 2: Add 0.5-1.5 parts of polyglycerol ricinoleate to 10-30 parts of soybean oil and stir for 5-15 minutes to obtain the oil phase for later use; Step 3: Add 1-5 parts of the fish skin gelatin mixture obtained in Step 1 to the oil phase obtained in Step 2 and mix evenly. Then add 0.1-1.0 parts of tannic acid, stir at 30-50℃ for 5-7 hours, centrifuge for 5-15 minutes, collect the precipitate, wash with water, and freeze dry to obtain the composite enzyme microcapsules.

7. The makeup remover wipes containing papain as described in claim 6, characterized in that, The preparation method of the modified hyaluronic acid includes the following steps, by weight: 50-150 parts of hyaluronic acid and 15,000-25,000 parts of water are mixed evenly, sterilized, cooled, inoculated with 0.1-0.5 wt% compound bacterial solution, and fermented at 40-45℃ for 8-12 hours; Centrifuge for 20-40 minutes, collect the supernatant, sterilize to obtain hyaluronic acid fermentation broth; mix 1-5 parts of modifier and 250-350 parts of water evenly, then add 10-50 parts of hyaluronic acid fermentation broth and mix evenly, freeze dry to obtain modified hyaluronic acid.

8. The makeup remover wipes containing papain as described in claim 7, characterized in that, The compound bacterial solution was obtained by mixing Lactobacillus rhamnosus and Lactobacillus plantarum, and the viable count of the solution was 0.5-1.5 × 10⁻⁶. 9 CFU / mL.

9. The makeup remover wipes containing papain as described in claim 7, characterized in that, The modifier is selected from at least one of xanthan gum, β-glucan, and konjac glucomannan.

10. A method for preparing a makeup remover wipe containing papain as described in any one of claims 1-9, characterized in that, Includes the following steps: Step 1: Weigh out the compound enzyme or compound enzyme microcapsules, sodium cocoyl glutamate, triglyceride decanoate, Centella asiatica extract, bisabolol, and water, then mix the substances to prepare a liquid composition. Step 2: Immerse the sterilized and dried substrate in the above liquid composition, then fold and seal it to obtain makeup remover wipes containing papain.

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