Compound essential oil for repairing sensitive skin as well as preparation method and application of compound essential oil

Abstract

The invention relates to compound essential oil for repairing sensitive skin as well as a preparation method and application of the compound essential oil, and relates to the field of daily chemical products. The compound essential oil is prepared from the following raw materials in parts by weight: 25 to 40 parts of red raspberry oil, 35 to 55 parts of calendula oil and 25 to 35 parts of cranberry oil. The natural compound oil prepared from the red raspberry oil, the calendula oil and the cranberry oil has a good anti-inflammatory effect and can effectively inhibit expression of inflammatory factors; the healing of human immortalized keratinocytes can be effectively promoted, and the effect of efficiently repairing skin cells is achieved.

Description

Technical Field

[0001] This invention relates to a compound essential oil for repairing sensitive skin, its preparation method, and its application; the technology pertains to the category of daily chemical products. Background Technology

[0002] Essential oils for sensitive skin refer to a class of essential oils suitable for use on sensitive skin. These oils typically possess gentle, soothing, and repairing properties, helping to alleviate discomfort and strengthen the skin's barrier function. These oils can reduce redness, itching, and stinging, leaving the skin feeling comfortable and calm. For example, chamomile essential oil has excellent soothing properties, effectively relieving skin sensitivity and redness. They can also help reduce inflammation, promote skin cell repair and regeneration, and enhance the skin's barrier function; for instance, lavender essential oil not only soothes the skin but also has antibacterial and anti-inflammatory effects, preventing skin infections and promoting skin recovery. These oils can also prevent moisture loss, increasing skin hydration and keeping the skin soft and smooth. Oils like rose and sandalwood have excellent moisturizing and nourishing effects.

[0003] Invention patent CN112022750B discloses a compound essential oil, its preparation method, and its application in anti-allergic and acne-removing cosmetics. This compound essential oil is composed of Plectranthus essential oil, Watercress essential oil, and Clerodendrum trichotomum essential oil in a ratio of 5-6:3-4:5-6.

[0004] Invention patent CN115531280A discloses a compound Dendrobium and Honeysuckle sensitive skin anti-allergy essential oil and its processing method, wherein the essential oil comprises the following weight components: 60-70 parts squalane, 10-20 parts corn germ oil, 15-25 parts wheat germ oil, 0.01-0.1 parts Dendrobium officinale extract, 0.01-0.1 parts licorice extract, 0.01-0.1 parts purslane extract, 0.01-0.1 parts Dictamnus dasycarpus root bark extract, 0.01-0.1 parts mulberry root bark extract, and 0.01-0.1 parts honeysuckle flower extract.

[0005] Invention patent CN109464311A discloses a whitening, anti-allergic, and anti-aging compound essential oil, which uses pine essential oil and oak essential oil as raw materials for the compound essential oil, supplemented with tea tree essential oil, violet essential oil, frankincense essential oil, palmarosa essential oil, orange essential oil and peppermint essential oil.

[0006] Invention patent CN117017793A discloses an essential oil emulsion for anti-allergy and soothing and its preparation process. The essential oil emulsion contains the following components: functional essential oil, fullerene, cell-activating water agent, hydrogenated lecithin, hyaluronic acid, panthenol, water-soluble vitamin E, and purified water.

[0007] Currently, there are many types of cosmetics for repairing skin, and their quality varies greatly. Therefore, further improving the repair of sensitive skin while also providing other benefits remains a technical problem that needs to be solved. Summary of the Invention

[0008] This invention provides a compound essential oil for repairing sensitive skin, its preparation method, and its application. The technology relates to the field of daily chemical products.

[0009] Specifically, the compound essential oil for repairing sensitive skin described in this invention comprises raspberry oil, calendula oil, and cranberry oil. The weight ratio of raspberry oil to calendula oil is 1:0.5-2.

[0010] In one embodiment, the ingredients of the compound essential oil, by weight, are: 25-40 parts red raspberry oil, 35-55 parts calendula oil, and 25-35 parts cranberry oil.

[0011] In one embodiment, the weight ratio of red raspberry oil to calendula oil in the compound essential oil is 1:0.8-1.5.

[0012] The present invention also provides a method for preparing the above-mentioned compound essential oil for repairing sensitive skin, including the step of mixing the raw materials.

[0013] The present invention also provides the application of the compound essential oil for repairing sensitive skin in the preparation of cosmetics.

[0014] In one embodiment, the cosmetic is a cosmetic for repairing sensitive skin.

[0015] In one embodiment, the cosmetic is a liquid, solid, or ointment formulation.

[0016] In one embodiment, the cosmetic is a face mask, serum, sunscreen lotion, sunscreen cream, lipstick, eye cream, eyeliner, foundation, makeup base, facial cleanser, or shower gel.

[0017] The present invention also provides a compound essential oil emulsion, the raw materials of which are: the compound essential oil for repairing sensitive skin, fermented extract, polyol, emulsifier and water.

[0018] In one embodiment, the preparation method of the fermentation extract includes: fermenting 0.1-3 parts by weight of Dendrobium chrysanthum stem and 0.1-3 parts by weight of Podocarpus macrophyllus leaf with Bacillus licheniformis and Bacillus belesii, and then obtaining the extract by filtration, concentration and drying.

[0019] In one embodiment, Bacillus licheniformis GDMCC NO.1.5713 and Bacillus belyssus GDMCC NO.1.135 are used. The optimal ratio of Bacillus licheniformis to Bacillus belyssus for fermentation is 1:2-3.5. Within this range, the fermentation of the two bacteria complements and synergistically enhances the efficacy of the fermented extract.

[0020] In one embodiment, the preparation method of the fermentation extract includes: pulverizing dried raw materials, mixing 0.1-3 parts by weight of Dendrobium chrysanthum stem powder and 0.1-3 parts by weight of Podocarpus macrophyllus leaf powder to obtain a mixture, mixing the mixture with water at a weight ratio of 1:5-45 to obtain the material, sterilizing it, then adding sucrose and inoculating it with Bacillus licheniformis and Bacillus belye at a ratio of 1:2-3.5 (the total amount of both is 10). 8 -10 11 (1 bacteria / g of material), fermented at 20-45℃ for 1-6 days, sterilized, and then obtained through filtration, concentration and drying.

[0021] In one embodiment, the raw materials of the compound essential oil emulsion are, by weight percentage: 0.5-10% compound essential oil, 0.5-10% fermentation extract, 20-50% polyol, 2-6% emulsifier and the balance water.

[0022] In one embodiment, the polyol includes at least one selected from propylene glycol, glycerol, butylene glycol, ethylene glycol, hexanediol, and pentanediol.

[0023] In one embodiment, the emulsifier includes at least one of Tween-20, Tween-40, and Tween-60.

[0024] The present invention also provides a method for preparing a compound essential oil emulsion, comprising: mixing compound essential oil with polyol to obtain an oil phase; mixing water, emulsifier, and fermentation extract to obtain an aqueous phase; and adding the oil phase to the aqueous phase and mixing to obtain a compound essential oil emulsion.

[0025] The beneficial effects of this invention are: The natural compound oil obtained by using red raspberry oil, calendula oil and cranberry oil in this invention has a good anti-inflammatory effect and can effectively inhibit the expression of inflammatory factors. Moreover, the combination of red raspberry oil and calendula oil can have a synergistic effect to enhance the anti-inflammatory and cell repair effects. The compound essential oil obtained by combining red raspberry oil and calendula oil in a weight ratio of 1:0.5-2 has better anti-inflammatory and skin repair effects.

[0026] This invention uses a natural compound oil derived from red raspberry oil, calendula oil, and cranberry oil, which can effectively promote the healing of immortalized keratinocytes and achieve a highly efficient skin cell repair effect.

[0027] The method for extracting and preparing essential oil raw materials according to the present invention is simple, and the compound essential oil has significant effects. The compound essential oil provided by the present invention has good application prospects.

[0028] This invention utilizes compound essential oils, fermented extracts, polyols, emulsifiers, and water to prepare an emulsion with excellent free radical scavenging effects, effectively combating oxidation and firming the skin. Changing the choice of fermentation bacteria during the preparation of the fermented extract reduces the free radical scavenging effect of the resulting emulsion. However, extracts obtained by fermenting with *Bacillus licheniformis* GDMCC NO.1.5713 and *Bacillus vesiliflorus* GDMCC NO.1.135 effectively enhance the free radical scavenging effect of the emulsion. The combined fermentation of *Bacillus licheniformis* GDMCC NO.1.5713 and *Bacillus vesiliflorus* GDMCC NO.1.135 synergistically increases the free radical scavenging effect of the fermented extract. Furthermore, a ratio of 1:2-3.5 between the two fermentation bacteria yields an even better emulsion. Detailed Implementation

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Unless otherwise specified, the materials and reagents used are commercially available.

[0030] I. Example 1 Red raspberry oil is prepared as follows: take red raspberry seeds, vacuum dry them at 40℃ until the water content is 2.96wt%, then place them in a screw press for pressing at room temperature, collect the oil, and filter it to obtain red raspberry oil.

[0031] Calendula oil is prepared as follows: Calendula flowers are crushed and then mixed with 75wt% ethanol at a weight ratio of 1:20. The mixture is extracted at 55℃ and 100rpm for 4 hours, filtered, and the filtrate is concentrated at 60℃ and 0.008MPa to a water content of 1.26wt%.

[0032] Cranberry oil is prepared as follows: Cranberry seeds are dried under vacuum at 40°C until the water content is 2.07 wt%, and then pressed in a screw press at room temperature. The oil is collected, filtered, and then cranberry oil is obtained.

[0033] The above raw materials were prepared into a compound oil according to the dosage ratio in Table 1.

[0034] Table 1: Composition of Compound Oil

[0035] The preparation method of the above compound oil is as follows: add red raspberry oil, calendula oil and cranberry oil in sequence to a container, and stir at 100 rpm for 0.5 h at room temperature to obtain the compound oil.

[0036] The performance of the above compound oil was tested: Test 1: Anti-inflammatory test Logarithmic growth phase mouse RAW264.7 cells were harvested at a density of 2.5 × 10⁻⁶ cells per well. 5 Cells were seeded at a density of [number] cells per well in 24-well plates, and 1 mL of DMEM medium was added. The plates were incubated at 37°C with 5% CO2 for 24 hours, after which the DMEM medium was replaced with fresh medium. The cells were then divided into a model group, an experimental group, and a positive control group. The model group received LPS only at a concentration of 0.5 μg / mL, the positive control group received LPS at a concentration of 0.5 μg / mL plus dexamethasone to a concentration of 130 μg / mL, and the experimental group received LPS at a concentration of 0.5 μg / mL plus compound oil to a concentration of 3.5 mg / mL. Each group was in triplicate. Cells were incubated at 37°C with 5% CO2 for 24 hours, and then the cell supernatant was collected. The levels of inflammatory factors TNF-α and IL-6 in the supernatant were measured using the ELISA kit instructions. The inhibition rate of TNF-α and IL-6 expression in each group compared to the model group was calculated using the following formula: Inhibition rate = 100% × (Model group content - Test group content) / Model group content. The results are shown in Table 1.

[0037] Table 1: Anti-inflammatory test

[0038] As shown in Table 1, the natural compound oils obtained by using red raspberry oil, calendula oil, and cranberry oil in this invention have good anti-inflammatory effects and can effectively inhibit the expression of inflammatory factors. A comparison of compound oils 1 and 4-5 shows that after changing the combination of red raspberry oil and calendula oil, compound oil 4-5, obtained by using only one of them, has a weaker anti-inflammatory effect than compound oil 1. This indicates that using red raspberry oil and calendula oil in combination in this invention can have a synergistic effect to enhance the anti-inflammatory effect. Furthermore, after changing the weight ratio of red raspberry oil and calendula oil in compound oils 1-3 and 6-8, the anti-inflammatory effect obtained by compound oil 1-3 with a ratio within the range of 1:0.5-2 is higher than that of compound oil 6-8 outside this range.

[0039] Test 2: Cell Repair Test 2 mL of DEME medium containing 10% FBS was added to each well of a 6-well plate, and HaCaT cells were seeded. The plates were incubated at 37°C and 5% CO2 until 85% confluence. Uniform scratches were made using a 200 μL pipette tip, followed by washing with PBS. The PBS was then removed, and fresh medium was added. Treatment was then divided into groups. The control group received only 2 mL of fresh medium, while the test group received 2 mL of fresh medium containing 0.6 mg / mL compound oil. Each group was in triplicate. All groups were incubated at 37°C and 5% CO2 for 24 hours. The healing of the scratches was observed under a microscope at different time points, and the healing rate was calculated based on the scratch area at different times. The healing rate was calculated as: Healing rate = 100% × (Scratch area at N hours - Scratch area at 0 hours) / Scratch area at 0 hours. The results are shown in Table 2.

[0040] Table 2: Healing Rate Test

[0041] As shown in Table 2, the natural compound oil obtained by using red raspberry oil, calendula oil, and cranberry oil in this invention can effectively promote the healing of immortalized keratinocytes and achieve a highly efficient skin cell repair effect. The use of red raspberry oil and calendula oil in this invention can enhance the skin cell repair effect through a synergistic combination, and the effect is even better when the ratio of the two is within the range of 1:0.5-2.

[0042] II. Example 2 Prepare a compound oil emulsion and test it.

[0043] Emulsion A: Raw materials, wt%: 5.5% compound oil 1, 6% fermentation extract, 10% ethylene glycol, 15% 1,3-butanediol, 10% glycerin, 2% Tween-20, 2% Tween-60 and balance water.

[0044] Fermentation Extract: Dried Dendrobium officinale stems and Podocarpus macrophyllus leaves were pulverized and mixed in a ratio of 2 parts by weight of Dendrobium officinale stem powder and 2 parts by weight of Podocarpus macrophyllus leaf powder. This mixture was then mixed with water at a weight ratio of 1:20 to obtain the final product. The mixture was sterilized, and then 2 wt% sucrose was added. The mixture was then inoculated with Bacillus licheniformis GDMCC NO.1.5713 and Bacillus belyssus GDMCC NO.1.135 at a ratio of 1:2 (total amount of both: 3 × 10⁻⁶). 9 (1 bacteria / g material), introduce sterile air at 0.4 vvm and ferment at 35℃ with stirring at 80 rpm for 3 days, then sterilize to stop fermentation, filter, concentrate under reduced pressure and freeze dry to a water content of 2.33 wt%.

[0045] The preparation method of the above emulsion is as follows: Compound oil 1, ethylene glycol, 1,3-butanediol and glycerin are stirred at 100 rpm at room temperature for 0.4 h to obtain mixture one; fermentation extract, Tween-20 and Tween-60 are added to water and stirred at 100 rpm at room temperature for 0.3 h to obtain mixture two; mixture one is added to mixture two and stirred at 100 rpm at room temperature for 0.5 h, sterilized, to obtain the emulsion.

[0046] Emulsion B: Raw materials, wt%: 6% compound oil 1, 5.8% fermentation extract, 5% ethylene glycol, 20% 1,3-butanediol, 8% glycerin, 1.5% Tween-20, 2.3% Tween-60 and balance water.

[0047] Fermentation Extract: Dried Dendrobium officinale stems and Podocarpus macrophyllus leaves were pulverized and mixed in a ratio of 1 part Dendrobium officinale stem powder to 1.5 parts Podocarpus macrophyllus leaf powder. This mixture was then mixed with water at a weight ratio of 1:25 to obtain the final material. The mixture was sterilized, and then 2.5 wt% sucrose was added. The mixture was then inoculated with Bacillus licheniformis GDMCC NO.1.5713 and Bacillus belyssus GDMCC NO.1.135 at a ratio of 1:3.5 (total amount of both: 3.2 × 10⁻⁶). 9 (1 bacteria / g material), introduce sterile air at 0.4 vvm and ferment at 37℃ with stirring at 80 rpm for 2.5 days, then sterilize to terminate fermentation, filter, concentrate under reduced pressure and freeze dry to a water content of 2.54 wt%.

[0048] The preparation method of this emulsion is the same as that of emulsion A.

[0049] Emulsion C: The difference from Emulsion A is that the fermentation bacteria used in the preparation of the fermentation extract is replaced with Acetobacter pasteurellii CICC22769, and the amount of fermentation bacteria used is 3×10⁻⁶. 9 One bacteria per gram of material, otherwise the same as emulsion A.

[0050] Emulsion D: The difference between Emulsion D and Emulsion A lies in the fermentation bacteria used in the preparation of the fermentation extract: Bacillus licheniformis GDMCC NO. 1.5713, and the amount of fermentation bacteria used is 3 × 10⁻⁶. 9 One bacteria per gram of material, otherwise the same as emulsion A.

[0051] Emulsion E: The difference between Emulsion E and Emulsion A lies in the fermentation bacteria used in the preparation of the fermented extract: Bacillus belye GDMCC NO.1.135, with a fermentation bacteria dosage of 3 × 10⁻⁶. 9 One bacteria per gram of material, otherwise the same as emulsion A.

[0052] Emulsion F differs from Emulsion A in that the fermentation bacteria used in the preparation of the fermentation extract are Bacillus licheniformis GDMCC NO.1.5713 and Bacillus belyssus GDMCC NO.1.135 in a ratio of 1:0.5, with a fermentation bacteria dosage of 3 × 10⁻⁶. 9 One bacteria per gram of material, otherwise the same as emulsion A.

[0053] Emulsion G: The difference between Emulsion G and Emulsion A lies in the fermentation bacteria used in the preparation of the fermentation extract: Bacillus licheniformis GDMCC NO.1.5713 and Bacillus belyssus GDMCC NO.1.135 in a ratio of 1:7, with a fermentation bacteria dosage of 3 × 10⁻⁶. 9 One bacteria per gram of material, otherwise the same as emulsion A.

[0054] The above-mentioned lotion was tested for its ability to scavenge free radicals, and its antioxidant and skin-firming effects were verified.

[0055] The absorbance at 520 nm was measured using an ELISA reader, and the scavenging rate of the sample against DPPH free radicals was calculated based on the absorbance. The specific experimental procedure is as follows: Preparation of test samples: DPPH solution: Prepare a working solution with a concentration of 0.15 mg / mL using 80 wt% ethanol solution.

[0056] The above emulsion was diluted with 50wt% ethanol solution to a weight concentration of 1% to obtain the emulsion dilution, and test samples were prepared.

[0057] Emulsion group: Prepare the emulsion group sample by adding 2.5 mL of DPPH solution and 0.5 mL of emulsion diluent. Blank group: Prepare the blank group sample by adding 2.5 mL of DPPH solution and 0.5 mL of 50 wt% ethanol solution. React the above samples at room temperature in the dark for half an hour, and measure the absorbance at 517 nm for each group; three parallel groups were set up. Calculate the DPPH free radical scavenging rate using the following formula: 100% × (Absorbance of blank group - Absorbance of emulsion group) / Absorbance of blank group, denoted as λ. 1% The results are shown in Table 3.

[0058] The above emulsion was diluted to a concentration of 2% by weight with 50 wt% ethanol solution to obtain a diluted emulsion solution, which was then used to prepare test samples. The DPPH free radical scavenging rate was then tested using the same method described above and denoted as λ. 2% The results are shown in Table 3.

[0059] Table 3: DPPH free radical scavenging rate

[0060] As shown in Table 3, the emulsion prepared using the fermented extract of this invention exhibits excellent free radical scavenging effects and effectively combats oxidation and firms the skin. A comparison of emulsions A and C reveals that changing the choice of fermentation bacteria reduces the free radical scavenging effect, while the extract obtained from the fermentation of the present invention effectively enhances the free radical scavenging effect. A comparison of emulsions A and DG shows that the combined fermentation of *Bacillus licheniformis* GDMCC NO.1.5713 and *Bacillus belyss* GDMCC NO.1.135 synergistically increases the free radical scavenging effect of the fermented extract. In particular, the emulsion obtained by fermenting *Bacillus licheniformis* and *Bacillus belyss* in a ratio of 1:2-3.5 shows even better results.

[0061] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details of the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solutions of the present invention, and these simple modifications all fall within the protection scope of the present invention. Furthermore, it should be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. To avoid unnecessary repetition, the present invention will not further describe the various possible combinations.

Claims

1. A compound essential oil for repairing sensitive skin, characterized in that, The raw materials are red raspberry oil, calendula oil and cranberry oil; wherein the weight ratio of the red raspberry oil and the calendula oil is 1:0.5-2.

2. The compound essential oil for repairing sensitive skin according to claim 1, characterized in that, The raw materials are red raspberry oil, calendula oil and cranberry oil; wherein the weight ratio of the red raspberry oil and the calendula oil is 1:0.5-2.

3. The compound essential oil for repairing sensitive skin according to claim 1, characterized in that, The raw materials are red raspberry oil, calendula oil and cranberry oil; wherein the weight ratio of the red raspberry oil and the calendula oil is 1:0.5-2.

4. The preparation method of the compound essential oil for repairing sensitive skin according to any one of claims 1-3, characterized in that, The raw materials are red raspberry oil, calendula oil and cranberry oil; wherein the weight ratio of the red raspberry oil and the calendula oil is 1:0.5-2. The raw materials are red raspberry oil, calendula oil and cranberry oil; wherein the weight ratio of the red raspberry oil and the calendula oil is 1:0.5-2.

6. Use according to claim 5, characterized in that, 5. Use of the compound essential oil for repairing sensitive skin according to any one of claims 1-3 in the preparation of a cosmetic product.

7. Use according to claim 5, characterized in that, The cosmetic product is a cosmetic product for repairing sensitive skin.

8. Use according to claim 5, characterized in that, The cosmetic product is in the form of a liquid, a solid or a paste.

9. A complex essential oil emulsion, characterized by, The cosmetic product is a mask, an essence, a sunscreen milk, a sunscreen cream, a lipstick, an eye cream, an eyeliner, a foundation, a sunscreen cream, a cleansing milk or a shower gel.

10. The complex essential oil emulsion according to claim 9, characterized in that, The raw materials are the compound essential oil for repairing sensitive skin according to any one of claims 1-3, a fermented extract, a polyol, an emulsifier and water; the preparation method of the fermented extract comprises: subjecting 0.1-3 parts by weight of Rhododendron spinuliferum and 0.1-3 parts by weight of Podocarpus neriifolius to fermentation by Bacillus licheniformis and Bacillus velezensis, and then filtering, concentrating and drying to obtain. The raw materials are red raspberry oil, calendula oil and cranberry oil; wherein the weight ratio of the red raspberry oil and the calendula oil is 1:0.5-2. 0.5-10% of the compound essential oil for repairing sensitive skin according to any one of claims 1-3, 0.5-10% of the fermented extract, 20-50% of the polyol, 2-6% of the emulsifier and the balance of water.

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