Natural plant extract composite preparation, preparation method thereof and application of natural plant extract composite preparation in skin oil control

Abstract

The invention belongs to the technical field of plant extract medicaments, and particularly discloses a natural plant extract compound preparation as well as a preparation method and application thereof in skin oil control. The preparation method comprises the following steps: adding the turmeric extract, the radix scutellariae extract, lecithin and cholesterol into absolute ethyl alcohol, dissolving to form an ethanol solution, dissolving the radix polygoni multiflori extract into ultrapure water to form an ultrapure water solution, adding the ethanol solution into the ultrapure water solution, stirring while adding, filtering to take supernatant, carrying out reduced pressure distillation to remove ethanol, centrifuging to take supernatant, and drying to obtain a finished product. Adding glucose, stirring and dissolving, and freeze-drying to obtain the turmeric extract, scutellaria baicalensis extract and polygonum multiflorum extract composite liposome. In the invention, the compound liposome of the turmeric extract, the scutellaria baicalensis extract and the polygonum multiflorum extract has the performance of slowly releasing the turmeric extract, the scutellaria baicalensis extract and the polygonum multiflorum extract, can obviously improve the cell intake of the turmeric extract, the scutellaria baicalensis extract and the polygonum multiflorum extract, and has a more excellent oil control effect.

Description

Technical Field

[0001] This invention belongs to the field of plant extract pharmaceutical technology, and particularly relates to natural plant extract compound preparations, their preparation methods, and their application in skin oil control. Background Technology

[0002] Oily skin is caused by excessive sebum secretion from the sebaceous glands. Excessive facial oil can lead to acne and negatively impact physical and mental health. Excessive scalp oil can cause dandruff, folliculitis, and seborrheic alopecia; visually, it makes hair appear thinner and may produce an unpleasant odor.

[0003] Oily skin, especially oily scalp, is primarily caused by the conversion of androgens into the more potent androgen dihydrotestosterone (DHT) via 5-α reductase. DHT stimulates the proliferation and secretion of sebaceous gland cells, leading to excessive sebum production. In addition, pathological causes of oily scalp include seborrheic dermatitis and folliculitis. Seborrheic dermatitis is a common skin condition characterized by redness, itching, oily scales (dandruff), and excessive sebum production. It is associated with the overgrowth of a fungus called Malassezia, which uses sebum as a nutrient source. Its metabolic products further irritate the scalp, causing inflammation and leading to increased oil production. Folliculitis is also a major cause of excessive sebum secretion. Its main mechanism is that excess sebum, when oxidized, activates the NF-κB pathway, producing a large number of inflammatory factors (such as TNF-α, IL-1β, and IL-6), causing redness, itching, and pain on the scalp (i.e., folliculitis), which in turn exacerbates sebum secretion. In summary, excessive scalp oil production is caused by a combination of factors, including overexpression of 5-α reductase, fungal growth, and excessive inflammation.

[0004] Currently, some advanced products have begun to design formulations around 5α-reductase inhibition, such as adding plant ingredients like Platycladus orientalis leaf extract and Ganoderma lucidum triterpenes. However, such single-component inhibitors have significant limitations in practical applications: firstly, the effect of single-component agents is limited; secondly, it is unclear whether simple physical mixing of multiple components will lead to antagonistic effects. Although studies have shown that curcumin has bidirectional regulatory activity on human 11β-HSD (hydroxysteroid dehydrogenase), can regulate hormone secretion and balance oil production, and has a certain inhibitory effect on most bacteria, with a relatively significant inhibitory effect. There are also reports that some flavonoids in Scutellaria baicalensis (such as baicalin) can directly act on human sebaceous gland cells, inhibiting excessive sebum secretion and improving the oily state of oily skin; and some extracts of Polygonum multiflorum have certain effects in inhibiting scalp sebum secretion and relieving scalp inflammation. However, there are currently no cases of combining these three components, therefore it is impossible to predict whether there is an antagonistic effect among them, nor can it be determined whether the combined use of the three components necessarily has a synergistic effect on skin oil control. Extracts from any plant can be of many types, and different extracts have different properties. It is currently impossible to determine whether all extracts, such as curcumin extract, scutellaria baicalensis extract, and polygonum multiflorum extract, have the aforementioned oil-controlling effects.

[0005] Therefore, there is an urgent need for a more comprehensive method to control scalp oil, addressing different scalp oil production mechanisms in order to achieve better oil control results. Summary of the Invention

[0006] This invention provides a compound preparation of natural plant extracts, its preparation method, and its application in skin oil control, mainly to solve the problem that the current treatments for skin oil control are not very effective.

[0007] To solve the above problems, the present invention adopts the following technical solution: The first aspect of this invention provides plant extract complex liposomes, their preparation methods, and applications.

[0008] One of the methods for preparing a complex liposome of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract includes the following steps: S1. Dissolve turmeric extract (CAS: 84775-52-0, the same below), scutellaria baicalensis extract (CAS: 94279-99-9, the same below), lecithin, and cholesterol in anhydrous ethanol to form an ethanol solution. Dissolve polygonum multiflorum extract (CAS: 82373-94-2, the same below) in ultrapure water to form an ultrapure aqueous solution. S2. Add the ethanol solution to the ultrapure water solution while stirring, filter and collect the supernatant (add the ethanol solution from S1 to the ultrapure water solution from S1 under stirring conditions and collect the supernatant). S3. Remove ethanol by vacuum distillation, centrifuge to collect the supernatant, add glucose and stir to dissolve, freeze dry to obtain a complex liposome of turmeric extract, scutellaria extract and fleeceflower root extract; The turmeric extract has CAS: 84775-52-0, the scutellaria extract has CAS: 94279-99-9, and the polygonum multiflorum extract has CAS: 82373-94-2.

[0009] Regarding some of the conditions in the above preparation method, the following options can be selected, and each feature can be selected individually: In some methods, in step S2: the ethanol solution in S1 is added to the ultrapure aqueous solution in S1 while stirring, and stirring continues after the addition is complete; stirring can continue for several hours after the addition is complete.

[0010] In some methods, in step S1, turmeric extract, scutellaria baicalensis extract, lecithin, and cholesterol are added to anhydrous ethanol and stirred at 50±5℃ to dissolve them into an ethanol solution. The mass ratio of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract to lecithin and cholesterol is 1:15:3, respectively.

[0011] In some methods, in step S2, an ethanol solution is added to the ultrapure aqueous solution prepared in step S1 at 50±5°C. More specifically, after preparing the ultrapure aqueous solution in step S1, the ultrapure aqueous solution is maintained at 50±5°C.

[0012] In some methods, in step S2: the supernatant is obtained by filtration through a 0.22 μm filter membrane; in step S3: ethanol is removed by vacuum distillation at 45°C, and the supernatant is pre-frozen at -80°C and then freeze-dried at -15°C. Furthermore, in step S3, ethanol is removed by vacuum distillation at 45°C, and the supernatant is obtained by centrifugation at 8000 rpm.

[0013] In some methods, the mass ratio of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract is 1:1:1. In some research examples of this invention, it has been demonstrated that preparing composite liposomes from turmeric, scutellaria baicalensis, and polygonum multiflorum extracts in a specific ratio can effectively overcome the antagonistic effects that may occur when the three are simply physically mixed, and produce a synergistic oil-controlling effect, exceeding the general expectations for the morphology of composite liposomes.

[0014] Secondly, regarding the liposomes containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract, these are primarily plant extract liposomes prepared by any of the aforementioned methods. This is mainly based on whether they constitute liposomes with the same composition and structure; it is not strictly required that they be prepared using the same method as described above. Any scheme with the same composition and structure as the plant extract liposomes of this invention and similar efficacy should be within the scope of this invention (the same applies to the hereinafter referred to as composite aqueous solutions). In the aforementioned preparation methods, in some specific examples, the proportions of each component can be referenced from the proportions in subsequent specific implementation examples. In some more specific examples of liposomes, the loading mass ratio of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract is 2.1:1.8:3.5. In other specific examples, this liposome containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract can reduce sebum secretion by more than 75% in the sebaceous gland sebaceous gland sebum secretion experiment of golden hamsters, and even reduce the amount by more than 80%.

[0015] Thirdly, regarding the application of liposomes containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract in the preparation of skin oil-controlling products. In skin oil-controlling products, their primary function is oil control. Of course, if added to other products, but still primarily functioning as oil-controlling products, they should still be classified as skin oil-controlling products.

[0016] In some methods, the aforementioned turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract complex liposomes are used in the preparation of skin oil-controlling products. In some specific examples, the turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract complex liposomes serve as the sole effective oil-controlling component in the skin oil-controlling product. This primarily refers to the absence of other traditional Chinese medicine extracts as oil-controlling components.

[0017] In some embodiments, the oil-controlling product for the skin is an oil-controlling product for the scalp. For example, when used as a shampoo, a complex liposome of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract can be used as the sole effective oil-controlling ingredient in the shampoo. In this case, no other herbal extracts are included as oil-controlling ingredients. However, if other synthetic oil-controlling ingredients are added, and these synthetic ingredients do not chemically react with the aforementioned turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract, then even if other synthetic oil-controlling ingredients are added, they should still be within the scope of this invention. The comparison between synthetic and natural herbal extracts is primarily based on the definition of synthetic.

[0018] The second aspect of this invention provides a composite aqueous solution of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract, as well as its preparation method and application.

[0019] One of them, the preparation method of plant extract compound aqueous solution, includes the following steps: S1. Mix turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract with hydroxypropyl-β-cyclodextrin and polyvinylpyrrolidone. S2. Add water and grind, then add water, stir and centrifuge, take the supernatant to obtain a compound aqueous solution of turmeric extract, scutellaria extract and fleeceflower root extract; The turmeric extract has CAS: 84775-52-0, the scutellaria extract has CAS: 94279-99-9, and the polygonum multiflorum extract has CAS: 82373-94-2.

[0020] In some methods, the mass ratio of turmeric extract, scutellaria extract, and polygonum multiflorum extract is 1:1:1.

[0021] Secondly, the application of the turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract compound aqueous solution prepared by the aforementioned method in the preparation of skin oil-control products. In specific applications, the turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract compound aqueous solution serves as the sole effective oil-controlling component in the skin oil-control product. This primarily means that no other traditional Chinese medicine extracts are included as oil-controlling components. For example, when used as a shampoo, the turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract compound aqueous solution can be used as the sole effective oil-controlling component in the shampoo, without including other traditional Chinese medicine extracts. However, if other artificially synthesized oil-controlling components are added, and these artificial components do not chemically react with the aforementioned turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract, then even if other artificially synthesized oil-controlling components are added, they should still be within the scope of this invention. Artificial synthesis is mainly compared with natural traditional Chinese medicine extracts.

[0022] In this disclosure, the components of the compound aqueous solution containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract synergistically enhance each other, thereby improving the oil control effect in many aspects. The compound liposomes containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract have the effect of sustained release of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract, which can effectively increase the cellular uptake of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract, and have a better oil control effect than turmeric extract, scutellaria baicalensis extract, polygonum multiflorum extract, and compound aqueous solution alone. Attached Figure Description

[0023] Figure 1 Images of sebaceous patches on the first and fourteenth days after drug administration in different groups; Figure 2 The volume of sebaceous plaques in golden hamsters was statistically analyzed on the first day and the fourteenth day after drug administration to different groups. Figure 3 H&E staining of sebaceous patches in golden hamsters fourteen days after drug administration to different groups; Figure 4Oil red staining of sebaceous spots in golden hamsters was performed 14 days after different groups were administered the drug. Detailed Implementation

[0024] The invention will be further described below with reference to specific research examples.

[0025] I. Preparation Method Compare with Example 1 Preparation of control solution: Mix 50g hydroxypropyl-β-cyclodextrin and 70g polyvinylpyrrolidone, add 30mL water and grind for 30 minutes, then add 400mL water, heat to 60℃ and stir for 1 hour to obtain control solution.

[0026] Example 1 Preparation of turmeric extract (CAS: 84775-52-0, the same below) aqueous solution: 0.5g turmeric extract was mixed with 50g hydroxypropyl-β-cyclodextrin and 70g polyvinylpyrrolidone, 30mL water was added and the mixture was ground for 30 minutes, then 400mL water was added, the mixture was heated to 60℃ and stirred for 1 hour, and finally centrifuged at 5000 rpm for 20 minutes. The supernatant was collected to obtain the turmeric extract aqueous solution.

[0027] Example 2 Preparation of Scutellaria baicalensis extract (CAS: 94279-99-9, the same below) aqueous solution: 0.5g of Scutellaria baicalensis extract was mixed with 50g of hydroxypropyl-β-cyclodextrin and 70g of polyvinylpyrrolidone, 30mL of water was added and the mixture was ground for 30 minutes, then 400mL of water was added, the mixture was heated to 60℃ and stirred for 1 hour, and finally centrifuged at 5000 rpm for 20 minutes. The supernatant was collected to obtain the aqueous solution of Scutellaria baicalensis extract.

[0028] Example 3 Preparation of aqueous extract of Polygonum multiflorum (CAS: 82373-94-2, the same below): 0.5g of Polygonum multiflorum extract was mixed with 50g of hydroxypropyl-β-cyclodextrin and 70g of polyvinylpyrrolidone, 30mL of water was added and the mixture was ground for 30 minutes, then 400mL of water was added, the mixture was heated to 60℃ and stirred for 1 hour, and finally centrifuged at 5000 rpm for 20 minutes. The supernatant was collected to obtain aqueous extract of Polygonum multiflorum.

[0029] Example 4 Preparation of a composite aqueous solution of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract: 0.5g of turmeric extract, 0.5g of scutellaria baicalensis extract, and 0.5g of polygonum multiflorum extract were mixed with 150g of hydroxypropyl-β-cyclodextrin and 210g of polyvinylpyrrolidone. 90mL of water was added and the mixture was ground for 30 minutes. Then, 340mL of water was added, and the mixture was heated to 60℃ and stirred for 1 hour. Finally, the mixture was centrifuged at 5000rpm for 20 minutes, and the supernatant was collected to obtain the aqueous solution of the mixture of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract.

[0030] Example 5 Preparation of aqueous liposomes containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract: 20 mg of turmeric extract, 20 mg of scutellaria baicalensis extract, 300 mg of lecithin, and 60 mg of cholesterol were added to 20 mL of anhydrous ethanol and heated to 50 °C with stirring to dissolve. 20 mg of polygonum multiflorum extract was dissolved in 20 mL of ultrapure water, and then heated to 50 °C. The ethanol solution was added to the ultrapure water in a 50 °C water bath while stirring continuously. After the addition was complete, stirring continued for 4 hours. The solution was then filtered through a 0.22 μm filter membrane, and the supernatant was collected. Ethanol was then removed by vacuum distillation at 45 °C, followed by centrifugation at 8000 rpm for 15 minutes. The supernatant was collected, and 300 mg of glucose was added and stirred to dissolve. The solution was pre-frozen at -80 °C for 3 hours, and then freeze-dried at -15 °C to obtain the liposomes containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract. The drug loading ratio of turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract in the composite liposomes was 2.1:1.8:3.5.

[0031] II. Testing Experiment (1) Mouse oil spot observation experiment Before the experiment, the fur on the back of the golden hamster was shaved with an electric shaver, and hair removal cream was used to remove the hair so that the two sebaceous gland patches on the back were clearly exposed. Under strong light, the maximum transverse diameter (DT) and maximum longitudinal diameter (DL) of the sebaceous gland patches on both sides were measured with calipers, the area (S) of the sebaceous gland patches was calculated, and the data was recorded by taking pictures.

[0032] S (mm) 2 = DT × DL Drug administration regimen: Golden hamster model mice were divided into 6 groups, with 3 mice in each group. The first group was the control group, which was treated with control example 1 once a day; the second to sixth groups were treated with the same amount of aqueous solution of examples 1 to 5 once a day; after 14 days of treatment, the oil spots on the backs of the mice were photographed and the area of ​​oil spots in each group was calculated. Then the golden hamsters were sacrificed and the skin on their backs was taken for histopathological analysis.

[0033] (2) H&E staining observation experiment of mouse oil spots After the last administration, 2cm×2cm sebaceous gland patch tissues were excised from golden hamsters, fixed with 10% formaldehyde fixative, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (HE) to observe the pathological changes of the sebaceous gland patch tissues.

[0034] (3) Observation experiment of oil spot and oil red staining in mice After the last administration, the skin tissue was fixed, dehydrated with sucrose, and frozen sections were prepared. Oil red staining was performed in the dark to observe the sebum secretion of the sebaceous glands.

[0035] (4) Cell uptake assay Test solution preparation: The traditional Chinese medicine complex liposomes prepared in Example 5 and the traditional Chinese medicine aqueous solutions prepared in Examples 1-4 were dispersed in ultrapure water to prepare an aqueous solution with a total concentration of 4 mg / mL of curcumin, baicalin, and stilbene glycosides. Then, the solution was diluted with RPMI 1640 medium to a total concentration of 20 μg / mL for each liposome test solution. The same concentration of free curcumin, baicalin, and stilbene glycosides was prepared as a control.

[0036] Cell uptake assay: 3T3 cells were seeded at a density of 100,000 cells per well in 12-well plates and cultured for 48 hours. Then, the cells were incubated with the above-prepared test solutions for 4 hours. Uptake was detected using flow cytometry. Three parallel groups were tested for each sample, and the average uptake was calculated.

[0037] III. Test Results like Figure 1 As shown, the aqueous solution of Control Example 1 had virtually no oil-controlling effect. The turmeric extract aqueous solution prepared in Example 1 and the scutellaria baicalensis extract aqueous solution prepared in Example 2, when used alone, both exhibited certain oil spot inhibition effects. While the Polygonum multiflorum extract prepared in Example 3 also had an inhibitory effect on oil spots when used alone, its inhibitory effect was the weakest. The compound aqueous solution of the three traditional Chinese medicine extracts prepared in Example 4 showed a more significant oil spot inhibition effect, significantly stronger than that of Examples 1 and 3, and also stronger than that of Example 2. This indicates that the combined use of the three extracts in the compound aqueous solution exerted a synergistic oil-controlling effect to a certain extent. Example 5 showed an even more significant oil spot inhibition effect, also significantly better than the compound aqueous solution of the three traditional Chinese medicine extracts prepared in Example 4. This preliminarily indicates that the composite liposomes of the three traditional Chinese medicines further improved the bioavailability of the three extracts, increased the synergistic effect of the three traditional Chinese medicines, and further enhanced the oil-controlling effect.

[0038] Further integration Figure 2 Table 1 shows a detailed statistical analysis of the oil spot volume. The results indicate that although Examples 1 and 3, compared to the aqueous solution of Control Example 1, inhibited the growth of oil spot volume after 14 days, they failed to prevent the oil spot volume from increasing relative to the original volume. This preliminarily shows that Examples 1 and 3 only inhibited oil spot growth and did not have the ability to reduce the oil spot volume relative to the original volume. In other words, the results shown in the table indicate that the turmeric extract of Example 1 and the Polygonum multiflorum extract of Example 3 did not have the ability to eliminate oil spots (reduce oil spot volume). Examples 2, 4, and 5 not only inhibited oil spot growth but also reduced oil spot volume. Example 2 had an effect on reducing oil spot volume, but it was not significant. Example 4 showed an improved effect on oil spot elimination compared to Example 2, and Example 5 significantly reduced oil spot volume.

[0039] Based on this, we initially hypothesize that Scutellaria baicalensis extract plays a role in reducing oil spots, but its effect alone is limited. However, when combined with Curcuma longa extract and Polygonum multiflorum extract, its ability to eliminate oil spots is significantly increased, showing a synergistic effect. These preliminary findings indicate that the synergistic effect of Scutellaria baicalensis extract with Curcuma longa extract and Polygonum multiflorum extract improves the control effect on oil spots. In particular, when Scutellaria baicalensis extract, Curcuma longa extract, and Polygonum multiflorum extract are combined and prepared into composite liposomes, the reduction in oil spot volume is significantly increased, and the effect is significantly better than other groups, demonstrating a more significant effect on eliminating oil spots.

[0040] like Figure 3 As shown in Tables 2 and 3, compared with Control Example 1, the sebaceous acini in Examples 1, 2, 4, and especially Example 5 were significantly reduced and loosely arranged, and the glands were smaller and more spindle-shaped. There were fewer cystic acini and surrounding glandular cells within the glands, indicating that the liposomes in Example 5 significantly inhibited sebaceous gland development, showing a significant difference from other groups. The turmeric extract in Example 1 alone did not show significant effects in inhibiting sebaceous acini and glandular cells. The reduced size and activity of sebaceous glands inhibit excessive sebum secretion and improve oil control, thus Example 5 showed the most significant oil control effect.

[0041] like Figure 4 As shown in Table 4, Oil Red O, as a lipid-soluble dye, can make lipids in tissues appear orange-red. Using Oil Red staining to observe sebum secretion in the sebaceous glands of golden hamsters, compared with Control Example 1, the orange-red lipid droplets around the acinar cells in Examples 1-5 all showed a decrease, with the most significant decrease in Example 5, where the orange-red lipid droplets around the acinar cells almost completely disappeared. This shows that the composite liposomes of Example 5 can most effectively regulate sebum secretion from the sebaceous glands, and its effect is significantly different from other groups. Example 4, compared with Control Example 1, also reduced the orange-red lipid droplets around the acinar cells. Surprisingly, compared with Examples 2 and 3, Example 4 did not show a significant advantage in controlling sebum secretion from the sebaceous glands, and even showed signs of deterioration. Preliminary results indicate that the mixed aqueous solution of the three Chinese herbal extracts did not have a synergistic effect in controlling sebum secretion. On the contrary, the mixed aqueous solution of the three Chinese herbal extracts may have an antagonistic effect in inhibiting sebum secretion. The inhibitory effect on sebum secretion was significantly weaker than that of the Scutellaria baicalensis extract in Example 2, indicating that the composite aqueous solution of the extracts still has some shortcomings. This suggests that the composite aqueous solution of the three Chinese herbal extracts may weaken certain aspects of its performance.

[0042] As shown in Table 5, the traditional Chinese medicine complex liposomes prepared in Example 5 have the effect of sustained release of the main active ingredients such as curcumin, baicalin, and stilbene glycosides, with the cumulative release amount within 70% after 48 hours.

[0043] As shown in Table 6, compared with free curcumin, baicalin, and stilbene glycosides, the liposomes prepared in Example 5 significantly increased the cellular uptake of curcumin extract, baicalin extract, and stilbene glycosides. The cellular uptake of Example 2 was inferior to that of Examples 1 and 3, and Example 4, as a composite aqueous solution, did not show a significant advantage over Examples 1-3. However, the relative cellular uptake of the composite liposome aqueous solution in Example 5 was significantly increased, indicating that Example 5 effectively improved the absorption of curcumin, baicalin, and stilbene glycosides, and enhanced bioavailability.

[0044] IV. Summary and Analysis The results of the above groups indicate that although turmeric extract (CAS:84775-52-0), scutellaria baicalensis extract (CAS:94279-99-9), and polygonum multiflorum extract (CAS:82373-94-2) each have certain effects in controlling oil production, their individual effects are limited. For example, turmeric extract and polygonum multiflorum extract do not have the ability to eliminate oil spots on their own; their ability to eliminate oil spots is only enhanced when combined with scutellaria baicalensis extract. Furthermore, in terms of inhibiting glandular cells, turmeric does not have a significant inhibitory effect, and the effects of scutellaria baicalensis extract and polygonum multiflorum extract are also not significant. However, the combined effect of all three is significantly improved compared to any single group. We also unexpectedly found that the compound aqueous solution of the three traditional Chinese medicines may deteriorate some of their oil-controlling properties (such as the inhibitory effect on sebaceous gland sebum secretion) after combination, and the combined effect is actually worse than that of using some of them individually. In summary: Regarding the aqueous solution containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract, while the combination of these three herbs exhibits synergistic effects in many aspects, its performance in some key oil-controlling properties is not particularly impressive, and even shows a decline in performance. However, the liposomes containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract demonstrate that each component plays a synergistic role in all the aforementioned oil-controlling properties, resulting in a significant improvement in efficacy. Surprisingly, it reverses the performance decline observed in the aqueous solution, making the oil-controlling function more comprehensive and superior, exhibiting excellent performance in both oil-controlling effect and biocompatibility.

[0045] Those skilled in the art will appreciate that various modifications to the above embodiments can be made without departing from the overall spirit and concept of the present invention. For any aspects not detailed herein, reference can be made to the prior art. All such modifications fall within the protection scope of the present invention. The protection scheme of the present invention is defined by the appended claims.

Claims

1. A method for preparing plant extract complex liposomes, characterized in that, Includes the following steps: S1. Dissolve turmeric extract, scutellaria baicalensis extract, lecithin, and cholesterol in anhydrous ethanol to form an ethanol solution. Dissolve polygonum multiflorum extract in ultrapure water to form an ultrapure aqueous solution. S2. Add the ethanol solution to the ultrapure water solution while stirring, filter and collect the supernatant. S3. Remove ethanol by vacuum distillation, centrifuge to collect the supernatant, add glucose and stir to dissolve, freeze dry to obtain a complex liposome of turmeric extract, scutellaria extract and fleeceflower root extract; The turmeric extract has CAS: 84775-52-0, the scutellaria extract has CAS: 94279-99-9, and the polygonum multiflorum extract has CAS: 82373-94-2.

2. The method for preparing plant extract complex liposomes according to claim 1, characterized in that, The mass ratio of turmeric extract, scutellaria extract, and polygonum multiflorum extract is 1:1:

1. In step S2, the ethanol solution in S1 is added to the ultrapure aqueous solution in S1 while stirring. After the addition is complete, stirring continues.

3. The method for preparing plant extract complex liposomes according to claim 1, characterized in that, In step S2: the supernatant is obtained by filtration using a 0.22 μm filter membrane.

4. The method for preparing plant extract complex liposomes according to claim 1, characterized in that, In step S3: Ethanol is removed by vacuum distillation at 45°C. For freeze drying, the mixture is first pre-frozen at -80°C and then freeze-dried at -15°C. Centrifugation is performed at 8000 rpm.

5. The turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract complex liposomes prepared by the method described in any one of claims 1 to 4.

6. The use of the turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract complex liposomes as described in claim 5 in the preparation of skin oil-controlling products.

7. The application according to claim 6, wherein, The liposome containing turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract is the sole effective ingredient in the skin oil-control product.

8. The application according to claim 6, wherein, The skin oil-control product mentioned is a scalp oil-control product.

9. A method for preparing a compound aqueous solution of plant extracts, characterized in that, Includes the following steps: S1. Mix turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract with hydroxypropyl-β-cyclodextrin and polyvinylpyrrolidone. S2. Add water and grind, then add water, stir and centrifuge, take the supernatant to obtain a compound aqueous solution of turmeric extract, scutellaria extract and fleeceflower root extract; The turmeric extract has CAS: 84775-52-0, the scutellaria extract has CAS: 94279-99-9, and the polygonum multiflorum extract has CAS: 82373-94-2.

10. The application of the turmeric extract, scutellaria baicalensis extract, and polygonum multiflorum extract compound aqueous solution prepared by the method of claim 9 in the preparation of skin oil-controlling products.

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