Nutrition injection for fruit tree trunks and preparation method thereof

Abstract

The invention relates to the technical field of nutrition injections, in particular to a nutrition injection for fruit tree trunks and a preparation method of the nutrition injection. The nutrition injection is prepared from the following raw materials: urea, a compound nutrient, an additive solution, cane sugar and water. In the preparation process of the nutrition injection for the fruit tree trunks, the ginkgo leaves, the rosemary and the lotus leaves are combined and added, and bilobalide components of the ginkgo leaves can promote division and elongation of root cells of fruit trees, so that the root systems of the fruit trees are more developed, and the water and nutrient absorption capacity of the fruit trees after transplanting is enhanced; rosmarinic acid has the effect of adjusting plant hormone balance and can stimulate fruit trees to root again at damaged positions of root systems after transplanting and improve the activity and absorption efficiency of the root systems, nuciferine is beneficial to improving the growth environment of the fruit tree root systems and promoting microbial activity of the fruit tree root systems, good conditions are provided for growth of the fruit tree root systems, and the yield of the fruit trees is increased. The yield and the transplanting survival rate of the fruit trees are effectively improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of nutrient injection, in particular to a nutrient injection for fruit tree trunk and a preparation method thereof. BACKGROUND

[0002] The fruit tree trunk nutrient injection is a preparation for supplementing the nutrition of fruit trees, which can be directly absorbed by the fruit trees and quickly supplement the nutrition by being injected into the trunk through a special needle.

[0003] In the prior art, the nutrient injection is generally suitable for the rapid nutrition supply of the transplanted fruit trees, however, the nutrition liquid directly injected into the branches and trunks has insufficient effect on the root nutrition supply of the fruit trees, so that the transplanted fruit trees cannot quickly recover the growth state, which affects the survival rate of the transplanted fruit trees. Based on this, the present application provides a nutrient injection for fruit tree trunk and a preparation method thereof. SUMMARY

[0004] The present application aims to provide a nutrient injection for fruit tree trunk and a preparation method thereof, the prepared nutrient injection for fruit tree trunk has good yield-increasing performance and excellent survival rate performance, and effectively improves the use performance of the nutrient injection.

[0005] To achieve the above-mentioned purpose, the present application provides the following technical scheme:

[0006] In the first aspect, the present application provides a nutrient injection for fruit tree trunk, which comprises the following raw materials: urea, compound nutrient agent, additive liquid, sucrose and water, and the weight percentage of each raw material is as follows: 4-6% urea, 8-10% compound nutrient agent, 6-10% additive liquid and 4-6% sucrose, and the balance is supplemented with water to 100%.

[0007] The preparation of the additive liquid comprises the following steps:

[0008] S1: preparation of mixed material, the raw materials of the mixed material include ginkgo leaves, rosemary, lotus leaves and water, the ginkgo leaves, rosemary and lotus leaves are pretreated before the preparation of the mixed material to obtain B component material and extraction liquid;

[0009] S2: preparation of culture medium, the raw materials of the culture medium include malt juice, bran, glucose and agar;

[0010] S3: fermentation treatment, the mixed material and the culture medium are subjected to fermentation treatment with a bacterial agent to prepare the additive liquid.

[0011] Further, the pretreatment method of the ginkgo leaves, rosemary and lotus leaves is as follows: the ginkgo leaves, rosemary and lotus leaves are washed and dried, then sent into an oven, the oven is set to dry at 40-50°C for 2-4h, then crushed, the particle size is 60-100μm, to obtain the crude material, the crude material is divided into A component material and B component material, the A component material and the ethanol aqueous solution are added into a water bath, the water bath is set to 50-60°C, and a magnetic stirrer is connected, the magnetic stirrer is set to rotate at 200-300r / min, constant temperature stirring treatment is carried out for 2-4h, the obtained product is added into a centrifuge, the centrifuge is set to 4000-6000r / min for 6-10min, the supernatant is obtained after centrifugal treatment, the supernatant is filtered to obtain the extract, and the pretreatment of the ginkgo leaves, rosemary and lotus leaves is completed, wherein the mass ratio of the A component material to the ethanol aqueous solution is 1:(4-6), and the mass concentration of the ethanol aqueous solution is 60-70%.

[0012] Further, the mass ratio of the A component material to the B component material is 1:(0.1-0.2), and the mass ratio of the extract to the additive is 1:(2-3).

[0013] Further, the preparation method of the mixed material is as follows: the extract and water are added into a mixer, the mixer is set to stir at 300-400r / min for 10-20min, and the obtained product is subjected to high-temperature sterilization treatment to obtain the mixed material.

[0014] Further, the preparation method of the culture medium is as follows: the wort and water with a mass of 2-4 times of the wort are added into a pot, boiled for 16-20min, then added into a water bath, the B component material, bran, glucose and agar are added into the water bath, the water bath is set to 50-60°C, and a magnetic stirrer is connected, the magnetic stirrer is set to rotate at 200-300r / min, constant temperature stirring treatment is carried out for 20-30min, and the obtained product is subjected to high-temperature sterilization treatment to obtain the culture medium.

[0015] Further, the fermentation treatment method is as follows: the mixed material and the culture medium are mixed and added into an incubator, the incubator is added with a bacterial agent and a supplement, the incubator is placed in a constant temperature shaker, the constant temperature shaker is set to 28-32°C and 180-200r / min, and cultured for 4-6h to obtain a culture solution, the culture solution is added into a refrigerated centrifuge, set to 2-4°C and 4000-6000r / min for 10-20min, the supernatant is obtained, and the supernatant is filtered to complete the fermentation treatment and obtain the additive.

[0016] Further, the mass of the bacterial agent is 0.06-0.08% of the mass of the mixed material, and the mass of the supplement is 1-3% of the mass of the mixed material, and the bacterial agent is an equal mass mixture of Paecilomyces lilacinus and actinomycetes.

[0017] Further, the supplement is prepared by mixing indole acetic acid, nanochitosan, vitamin C, naphthalene acetic acid sodium and deionized water, and the mass ratio of each raw material is 1:(0.6-0.8):(1.4-1.6):(0.6-0.8):(2-4).

[0018] Further, the compound nutrient agent is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, fulvic acid and water, and the mass ratio of each raw material is 1:(0.4-0.6):(0.2-0.4):1:(0.1-0.3):(0.1-0.3):(0.2-0.4):(0.8-1.2):(6-8).

[0019] In the second aspect, the application further provides a preparation method of the fruit tree stem nutrient injection, which comprises the following steps: weighing urea, the compound nutrient agent, the additive liquid, sucrose and water according to the requirements and adding them into a mixer, setting the mixer to stir at 600-800 r / min for 20-30 min, and preparing the fruit tree stem nutrient injection.

[0020] Compared with the prior art, the application has the following beneficial effects:

[0021] 1. In the preparation process of the fruit tree stem nutrient injection, the combination of ginkgo leaves, rosemary and lotus leaves is added, wherein the ginkgo biloba lactone component of the ginkgo leaves can promote the division and elongation of fruit tree root cells, so that the root system of the fruit tree is more developed, the absorption capacity of the fruit tree for water and nutrients after transplanting is enhanced, the rosemary acid has the function of regulating the balance of plant hormones and can stimulate the fruit tree to regenerate roots at the damaged position of the root system after transplanting, improve the activity and absorption efficiency of the root system, the lotus leaf alkaloid component helps to improve the growth environment of the fruit tree root system, promotes the microbial activity of the fruit tree root system, provides good conditions for the growth of the fruit tree root system, and thus effectively improves the yield and transplanting survival rate of the fruit tree.

[0022] 2. In the preparation process of the fruit tree stem nutrient injection, the culture medium provides nutrients and energy, and the use of the bacterial agent for fermentation treatment of the extract, after the fermentation treatment, the paecilomyces lilacinus can secrete plant growth hormones to promote root development, the actinomycetes can produce antibiotics to inhibit pathogenic microorganisms, and at the same time, decompose organic matter to release nutrients, which has a good growth recovery effect on the fruit tree after transplanting, indole acetic acid and naphthalene acetic acid sodium can directly stimulate plant cell division and root formation of the fruit tree, and a large amount of antioxidant substances in the fermentation product can reduce the oxidative damage of the fruit tree during transplanting, effectively improving the survival rate of the fruit tree after transplanting. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1The application provides a fruit tree stem nutrition injection and a preparation method thereof. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the application will be clearly and completely described below with reference to the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments of the application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the application.

[0025] It should be noted that the raw materials used in the following embodiments are commercially available.

[0026] Embodiment one: the raw materials include urea, a compound nutrient agent, an additive, sucrose and water, and the weight percentage of each raw material is as follows: 4% urea, 8% compound nutrient agent, 6% additive, 4% sucrose, and the rest is water to make up to 100%;

[0027] The preparation of the additive includes the following steps:

[0028] S1: mixture preparation, the raw materials of the mixture include ginkgo leaves, rosemary, lotus leaves and water, the ginkgo leaves, rosemary and lotus leaves are pretreated before the mixture preparation to obtain B component material and an extract;

[0029] The pretreatment method of the ginkgo leaves, rosemary and lotus leaves is as follows: the ginkgo leaves, rosemary and lotus leaves are washed and dried, then are sent into an oven, the oven is set to 40 DEG C for drying treatment for 2 h, and then are crushed, the particle size of the crushed product is 60 mu m, to obtain a coarse material, the coarse material is divided into A component material and B component material, the A component material and an ethanol aqueous solution are added into a water bath pot, the water bath pot is set to 50 DEG C, and a magnetic stirrer is connected at the same time, the magnetic stirrer is set to 200 r / min, and constant temperature stirring treatment is performed for 2 h, the obtained product is added into a centrifuge, the centrifuge is set to 4000 r / min for centrifugal treatment for 6 min, the supernatant is obtained after the centrifugal treatment, and the supernatant is filtered to obtain the extract, and the pretreatment of the ginkgo leaves, rosemary and lotus leaves is completed, wherein the mass ratio of the A component material to the ethanol aqueous solution is 1:4, the mass concentration of the ethanol aqueous solution is 60%, the mass ratio of the A component material to the B component material is 1:0.1, and the mass ratio of the extract to the additive is 1:2;

[0030] The preparation method of the mixture is as follows: the extract and water are added into a mixer, the mixer is set to 300 r / min for stirring treatment for 10 min, and the obtained product is subjected to high-temperature sterilization treatment to obtain the mixture;

[0031] S2: culture medium preparation, the raw materials of the culture medium include malt juice, bran, glucose and agar;

[0032] The method for preparing the culture medium is as follows: malt extract is added into a pot with 2 times of its mass of water, boiled for 16 min, and then added into a water bath after being cooled to room temperature; B component material, bran, glucose and agar are added into the water bath, the temperature of the water bath is set to 50℃, a magnetic stirrer is connected at the same time, the rotating speed of the magnetic stirrer is set to 200 r / min, and the obtained product is treated by constant temperature stirring for 20 min, and then sterilized at high temperature to prepare the culture medium;

[0033] S3: fermentation treatment, the mixture and the culture medium are mixed with a bacterial agent to prepare an additive solution;

[0034] The method for fermentation treatment is as follows: the mixture and the culture medium are mixed and then added into a culture box, a bacterial agent and a supplement are added into the culture box, the culture box is placed in a constant temperature shaker, the temperature of the constant temperature shaker is set to 28℃, the rotating speed is set to 180 r / min, and the mixture is cultured for 4 h to obtain a culture solution; the culture solution is added into a refrigerated centrifuge, the temperature of the refrigerated centrifuge is set to 2℃, the rotating speed is set to 4000 r / min, and the culture solution is centrifuged for 10 min to obtain a supernatant; the supernatant is filtered to complete the fermentation treatment and prepare the additive solution, wherein the mass of the bacterial agent is 0.06% of the mass of the mixture, the mass of the supplement is 1% of the mass of the mixture, the bacterial agent is an equal mass mixture of Paecilomyces lilacinus and actinomycetes, and the supplement is prepared by mixing indole-3-acetic acid, nano-chitosan, vitamin C, naphthalene acetic acid sodium and deionized water, and the mass ratio of the raw materials is 1:0.6:1.4:0.6:2;

[0035] The composite nutrient agent is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, fulvic acid and water, and the mass ratio of the raw materials is 1:0.4:0.2:1:0.1:0.1:0.2:0.8:6;

[0036] The preparation method of the nutrient injection solution for fruit tree trunks comprises the following steps: weighing urea, a composite nutrient agent, an additive solution, sucrose and water according to the requirements, adding them into a mixer, setting the mixer to stir at 600 r / min for 20 min, and preparing the nutrient injection solution for fruit tree trunks.

[0037] Example 2 comprises the following raw materials: urea, a composite nutrient agent, an additive solution, sucrose and water, and the weight percentage of each raw material is as follows: 5% urea, 9% composite nutrient agent, 8% additive solution, 5% sucrose, and the balance is made up to 100% by water;

[0038] The preparation of the additive solution comprises the following steps:

[0039] S1: preparation of the mixture, the raw materials of the mixture include ginkgo leaves, rosemary, lotus leaves and water, the ginkgo leaves, rosemary and lotus leaves are pretreated before the preparation of the mixture to obtain B component material and an extract;

[0040] The pretreatment method of ginkgo leaves, rosemary and lotus leaves is as follows: the ginkgo leaves, rosemary and lotus leaves are washed and dried, then sent into an oven, the oven is set at 45℃ for drying treatment for 3h, then crushed, the particle size is 80μm, to obtain the coarse material, the coarse material is divided into A component material and B component material, the A component material and the ethanol aqueous solution are added into a water bath, the water bath is set at 55℃, at the same time, a magnetic stirrer is connected, the magnetic stirrer is set at 250r / min, constant temperature stirring treatment for 3h, the obtained product is added into a centrifuge, the centrifuge is set at 5000r / min for centrifugal treatment for 8min, after centrifugal treatment, the supernatant is obtained, the supernatant is filtered to obtain the extract, the pretreatment of ginkgo leaves, rosemary and lotus leaves is completed, wherein, the mass ratio of the A component material to the ethanol aqueous solution is 1:5, the mass concentration of the ethanol aqueous solution is 65%, the mass ratio of the A component material to the B component material is 1:0.15, the mass ratio of the extract to the additive is 1:2.5;

[0041] The method for preparing the mixture is as follows: the extract and water are added into a mixer, the mixer is set at 350r / min for stirring treatment for 15min, the obtained product is subjected to high temperature sterilization treatment, to obtain the mixture;

[0042] S2: culture medium preparation, the raw materials of the culture medium include malt juice, bran, glucose and agar;

[0043] The method for preparing the culture medium is as follows: the malt juice and water with 3 times the mass of the malt juice are added into a pot, boiled for 18min, then cooled to room temperature and added into a water bath, the B component material, bran, glucose and agar are added into the water bath, the water bath is set at 55℃, at the same time, a magnetic stirrer is connected, the magnetic stirrer is set at 250r / min, constant temperature stirring treatment for 25min, the obtained product is subjected to high temperature sterilization treatment, to obtain the culture medium;

[0044] S3: fermentation treatment, the mixture, the culture medium and the bacterial agent are subjected to fermentation treatment, to obtain the additive;

[0045] The method for fermentation treatment is as follows: the mixture and the culture medium are mixed and added into an incubator, the bacterial agent and the supplement are added into the incubator, the incubator is placed in a constant temperature shaker, the constant temperature shaker is set at 30℃, 190r / min, and cultured for 5h, to obtain the culture liquid, the culture liquid is added into a refrigerated centrifuge, set at 3℃, 5000r / min for centrifugal treatment for 15min, the supernatant is obtained, the supernatant is filtered, the fermentation treatment is completed, and the additive is obtained, wherein, the mass of the bacterial agent is 0.07% of the mass of the mixture, the mass of the supplement is 2% of the mass of the mixture, the bacterial agent is an equal mass mixture of paecilomyces lilacinus and actinomycete, the supplement is prepared by mixing indole acetic acid, nano-chitosan, vitamin C, naphthalene acetic acid sodium and deionized water, and the mass ratio of the raw materials is 1:0.7:1.5:0.7:3;

[0046] The composite nutrient agent is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, fulvic acid and water, and the mass ratio of each raw material is 1:0.5:0.3:1:0.2:0.2:0.3:1:7;

[0047] The preparation method of the fruit tree trunk nutrient injection liquid comprises the following steps: weighing urea, a composite nutrient agent, an additive, sucrose and water according to the requirements, and adding them into a mixer; the mixer is set to stir at 700 r / min for 25 min to prepare the fruit tree trunk nutrient injection liquid.

[0048] Example three comprises the following raw materials: urea, a composite nutrient agent, an additive, sucrose and water, and the weight percentage of each raw material is: 6% urea, 10% composite nutrient agent, 10% additive, 6% sucrose, and the balance is supplemented with water to 100%.

[0049] The preparation of the additive comprises the following steps:

[0050] S1: mixture preparation, the raw materials of the mixture include ginkgo leaves, rosemary, lotus leaves and water, the ginkgo leaves, rosemary and lotus leaves are pretreated before the mixture preparation to obtain B component material and an extract;

[0051] The pretreatment method of the ginkgo leaves, rosemary and lotus leaves is as follows: the ginkgo leaves, rosemary and lotus leaves are washed and dried, then sent into an oven, the oven is set to dry at 50℃ for 4h, and then crushed to a particle size of 100μm to obtain a coarse material, the coarse material is divided into A component material and B component material, the A component material and an ethanol aqueous solution are added into a water bath, the water bath is set to a temperature of 60℃, and a magnetic stirrer is connected at the same time, the magnetic stirrer is set to a rotating speed of 300r / min, and constant temperature stirring is carried out for 4h, the obtained product is added into a centrifuge, the centrifuge is set to 6000r / min for centrifugal treatment for 10min, the supernatant is obtained after centrifugal treatment, and the supernatant is filtered to obtain the extract, thus completing the pretreatment of the ginkgo leaves, rosemary and lotus leaves, wherein the mass ratio of the A component material and the ethanol aqueous solution is 1:6, the mass concentration of the ethanol aqueous solution is 70%, the mass ratio of the A component material and the B component material is 1:0.2, and the mass ratio of the extract and the additive is 1:3;

[0052] The mixture preparation method is as follows: the extract and water are added into a mixer, the mixer is set to stir at 400r / min for 20min, and the obtained product is subjected to high-temperature sterilization treatment to obtain the mixture;

[0053] S2: culture medium preparation, the raw materials of the culture medium include malt juice, bran, glucose and agar;

[0054] The method for preparing the culture medium is as follows: malt extract and water with a mass of 4 times of the malt extract are added into a pot, boiled for 20 min, and then added into a water bath pot after being cooled to room temperature; the water bath pot is set to a temperature of 60 DEG C, and a magnetic stirrer is connected at the same time, and the magnetic stirrer is set to a rotating speed of 300 r / min; the obtained product is treated by constant temperature stirring for 30 min, and then sterilized at high temperature to obtain the culture medium.

[0055] S3: fermentation treatment, the mixture, the culture medium and the bacterial agent are mixed to obtain the additive liquid;

[0056] The method for the fermentation treatment is as follows: the mixture and the culture medium are mixed and then added into a culture box; the bacterial agent and the supplement are added into the culture box; the culture box is placed in a constant temperature shaker, which is set to a temperature of 32 DEG C and a rotating speed of 200 r / min; the culture liquid is obtained after being cultured for 6 h; the culture liquid is added into a refrigerated centrifuge, which is set to a temperature of 4 DEG C and a rotating speed of 6000 r / min; the supernatant is obtained after being centrifuged for 20 min; the supernatant is filtered to obtain the additive liquid; the mass of the bacterial agent is 0.08% of the mass of the mixture; the mass of the supplement is 3% of the mass of the mixture; the bacterial agent is an equal mass mixture of Paecilomyces lilacinus and actinomycete; the supplement is prepared by mixing indole-3-acetic acid, nano-chitosan, vitamin C, naphthalene acetic acid sodium and deionized water; and the mass ratio of the raw materials is 1:0.8:1.6:0.8:4.

[0057] The composite nutrient agent is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, fulvic acid and water; and the mass ratio of the raw materials is 1:0.6:0.4:1:0.3:0.3:0.4:1.2:8.

[0058] The preparation method of the nutrient injection liquid for fruit tree trunks comprises the following steps: weighing urea, the composite nutrient agent, the additive liquid, sucrose and water according to the requirements, and adding them into a mixer; the mixer is set to a rotating speed of 800 r / min for stirring treatment for 30 min to obtain the nutrient injection liquid for fruit tree trunks.

[0059] Comparative Example 1, the difference between the comparative example and example 1 is that an equal amount of indole-3-acetic acid is used to replace the supplement in the comparative example.

[0060] Comparative Example 2, the difference between the comparative example and example 1 is that the comparative example does not contain the supplement.

[0061] Comparative Example 3, the difference between the comparative example and example 1 is that an equal amount of malt extract is used to replace the additive liquid in the comparative example.

[0062] Comparative Example 4, the difference between the comparative example and example 1 is that the comparative example does not contain the additive liquid.

[0063] Performance test: the performance test of the fruit tree trunk nutrient injection prepared by example one, example two, example three, comparative example one, comparative example two, comparative example three and comparative example four is carried out, and the test data obtained are recorded in the following table:

[0064]

[0065] In the performance test, the fruit tree trunk nutrient injection prepared by example one, example two, example three, comparative example one, comparative example two, comparative example three and comparative example four is injected into the transplanted apple trees in batches, the survival rate of the apple trees is recorded 100 days after the transplantation, each batch of apple trees is 100, and the yield of each batch of apple trees is recorded in a planting cycle after the transplantation, and the average yield per plant is calculated.

[0066] It can be seen that the yield performance and survival rate data of the fruit tree trunk nutrient injection prepared by comparative examples one, two, three and four are lower than those of examples one, two and three; this shows that: in the preparation process of the fruit tree trunk nutrient injection, the combination of ginkgo leaves, rosemary and lotus leaves is added, wherein the ginkgolide component of ginkgo leaves can promote the division and elongation of fruit tree root system cells, so that the fruit tree root system is more developed, the absorption capacity of the fruit tree to water and nutrients after transplantation is enhanced, the rosemary acid has the function of regulating the balance of plant hormones, can stimulate the fruit tree to regenerate roots at the damaged position of the root system after transplantation, improve the activity and absorption efficiency of the root system, the lotus leaf alkaloid component helps to improve the growth environment of the fruit tree root system, promotes the microbial activity of the fruit tree root system, provides good conditions for the growth of the fruit tree root system, and effectively improves the yield and survival rate of the fruit tree after transplantation;

[0067] In the preparation process of the fruit tree trunk nutrient injection, the culture medium provides nutrients and energy, and the fermentation treatment of the extract liquid is carried out by using the bacterial agent, after the fermentation treatment, the paecilomyces lilacinus can secrete plant growth hormones, play a role in promoting root system development, actinomycetes can produce antibiotics, inhibit pathogenic microorganisms, and decompose organic matter to release nutrients, have a good growth recovery effect on the transplanted fruit trees, indole acetic acid and naphthalene acetic acid can directly stimulate plant cell division and fruit tree root system generation, and a large amount of antioxidant substances in the fermentation product can reduce the oxidative damage of the fruit tree during transplantation, and effectively improve the survival rate of the fruit tree after transplantation.

[0068] It can be seen from the comparison and analysis of the related data in the table that the fruit tree trunk nutrient injection prepared by the application not only has good yield performance, but also has excellent survival rate performance. Therefore, it is shown that the fruit tree trunk nutrient injection provided by the application has a wider market prospect and is more suitable for promotion.

[0069] In the description of the specification, reference to "one embodiment", "an example", "a specific example" or the like means that a particular feature, structure, material or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. The appearances of the phrases "in one embodiment", "an example", "a specific example" or the like in various places in the specification are not necessarily referring to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics can be combined in any suitable manner in one or more embodiments or examples.

[0070] The preferred embodiments of the application disclosed above are only to help explain the application. The preferred embodiments do not describe all the details of the application and limit the application to the specific embodiments described. Obviously, many modifications and variations can be made in light of the contents of the specification. The specification selects and specifically describes these embodiments in order to better explain the principles and practical application of the application, so that those skilled in the art can well understand and utilize the application. The application is limited only by the claims and their full scope and equivalents.

Claims

1. A nutrient injection solution for fruit tree trunk, characterized by comprising: The raw material composition comprises urea, compound nutrient agent, additive liquid, sucrose and water, and the weight percentage of each raw material is as follows: 4-6% urea, 8-10% compound nutrient agent, 6-10% additive liquid, 4-6% sucrose, and the rest is water to make up to 100%. ​ The preparation of the additive liquid comprises the following steps: S1: mixture preparation, the raw materials of the mixture comprise ginkgo leaves, rosemary, lotus leaves and water, the ginkgo leaves, rosemary and lotus leaves are pretreated before the mixture preparation, to obtain B component material and extraction liquid; S2: culture medium preparation, the raw materials of the culture medium comprise malt juice, bran, glucose and agar; S3: fermentation treatment, the mixture, culture medium and bacterial agent are mixed for fermentation treatment, to obtain the additive liquid.

2. The nutrient injection solution for fruit tree trunk according to claim 1, characterized by, The pretreatment method of the ginkgo leaves, rosemary and lotus leaves is as follows: the ginkgo leaves, rosemary and lotus leaves are washed and dried, then are sent into an oven, the oven is set at 40-50 DEG C for drying treatment for 2-4 h, then are crushed, the particle size of the crushed product is 60-100 mu m, to obtain coarse material, the coarse material is divided into A component material and B component material, the A component material and ethanol aqueous solution are added into a water bath pot, the water bath pot is set at 50-60 DEG C, and a magnetic stirrer is connected, the magnetic stirrer is set at 200-300 r / min, and the obtained product is treated by constant temperature stirring for 2-4 h, then is added into a centrifuge, the centrifuge is set at 4000-6000 r / min for centrifugal treatment for 6-10 min, the supernatant is obtained after the centrifugal treatment, and the supernatant is filtered, to obtain the extraction liquid, and the pretreatment of the ginkgo leaves, rosemary and lotus leaves is completed, wherein, the mass ratio of the A component material to the ethanol aqueous solution is 1:(4-6), and the mass concentration of the ethanol aqueous solution is 60-70%.

3. The nutrient injection solution for fruit tree trunk according to claim 2, characterized by, The mass ratio of the A component material to the B component material is 1:(0.1-0.2), and the mass ratio of the extraction liquid to the additive liquid is 1:(2-3).

4. The nutrient injection solution for fruit tree trunk according to claim 1, characterized by, The mixture preparation method is as follows: the extraction liquid and water are added into a mixer, the mixer is set at 300-400 r / min for stirring treatment for 10-20 min, and the obtained product is treated by high temperature sterilization, to obtain the mixture.

5. The nutrient injection solution for fruit tree trunk according to claim 1, characterized by, The culture medium preparation method is as follows: the malt juice and water with 2-4 times the mass of the malt juice are added into a pot, boiled for 16-20 min, then are added into a water bath pot, the B component material, bran, glucose and agar are added into the water bath pot, the water bath pot is set at 50-60 DEG C, and a magnetic stirrer is connected, the magnetic stirrer is set at 200-300 r / min, and the obtained product is treated by constant temperature stirring for 20-30 min, then is treated by high temperature sterilization, to obtain the culture medium.

6. The nutrient injection solution for fruit tree trunk according to claim 1, characterized by, The fermentation treatment method is as follows: the mixture and the culture medium are mixed, then are added into an incubator, a bacterial agent and a supplement are added into the incubator, the incubator is placed in a constant temperature shaker, the constant temperature shaker is set at 28-32 DEG C and 180-200 r / min, and the mixture is cultured for 4-6 h, to obtain a culture liquid, the culture liquid is added into a refrigerated centrifuge, set at 2-4 DEG C and 4000-6000 r / min for centrifugal treatment for 10-20 min, the supernatant is obtained, and the supernatant is filtered, to complete the fermentation treatment, and obtain the additive liquid.

7. The nutrient injection solution for fruit tree trunk according to claim 6, characterized by, The quality of the bacteria agent is 0.06-0.08% of the mixed material, and the quality of the supplement is 1-3% of the mixed material.

8. The nutrient injection solution for fruit tree trunk according to claim 6, characterized by, The supplement is prepared by mixing indole acetic acid, nano-chitosan, vitamin C, naphthalene acetic acid sodium and deionized water, and the mass ratio of each raw material is 1:(0.6-0.8):(1.4-1.6):(0.6-0.8):(2-4).

9. The nutrient injection solution for fruit tree trunk according to claim 1, characterized by, The compound nutrient agent is prepared by mixing ferrous sulfate, zinc sulfate, manganese sulfate, potassium nitrate, calcium nitrate, potassium hydroxide, potassium dihydrogen phosphate, fulvic acid and water, and the mass ratio of each raw material is 1:(0.4-0.6):(0.2-0.4):1:(0.1-0.3):(0.1-0.3):(0.2-0.4):(0.8-1.2):(6-8).

10. A method for preparing the nutrient injection solution for fruit tree trunk according to any one of claims 1 to 9, characterized by, The method comprises the following steps: weighing urea, compound nutrient agent, additive liquid, sucrose and water according to requirements, and adding them into a mixer, setting the mixer to stir at 600-800 r / min for 20-30 min, and preparing the fruit tree stem nutrient injection.

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