Intelligent three-gas culture device with micro-pressure maintaining function

Abstract

The application relates to the technical field of culture devices, and particularly discloses an intelligent three-gas culture device with micro-pressure maintaining function, which comprises a blocking mechanism, a shaker mechanism arranged at the bottom of the inner wall of a three-gas culture box and an auxiliary mechanism arranged at the top of the inner wall of the three-gas culture box. The culture bottle and the cells are separated from the shaker mechanism and the gas guide mechanism, so that a large number of structures and the cells are not in the same environment, the difficulty of cleaning the inside of the three-gas culture box is reduced, cross infection is reduced, the culture of different cells and tissues is facilitated, the shaking of the tray and the culture bottle driven by the shaker mechanism is not affected, in addition, the gas can flow in the three-gas culture box, so that the gas in the three-gas culture box is uniformly distributed, all the cultures are ensured to be in the same condition, and the uneven growth of the cells or the abnormal metabolism caused by the local concentration difference is avoided.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of culture devices, in particular to an intelligent three-gas culture device with micro-pressure maintaining function. BACKGROUND

[0002] The three-gas culture device is mainly used for simultaneously controlling the temperature, humidity, oxygen, carbon dioxide and nitrogen concentration in the culture environment, and provides precise micro-environment for cell culture, microbial fermentation, tissue engineering and other experiments.

[0003] The background technology of the disclosure No. CN114456934B discloses an intelligent automatic culture device for tissue culture, and the problem proposed in the background technology is that the shock force of the existing culture device is usually fixed, and the shock force cannot be automatically adjusted when culturing different amounts of tissue, and the shock force is too large to cause the overflow of the material in the culture dish, and the shock force is too small to cause uneven shaking, which is inconvenient to use.

[0004] Based on the existing technology, the following problems exist:

[0005] The existing three-gas culture device usually sets a shaker in its interior, which is in the same environment as the culture dish, cells and culture medium, etc. After completing a culture, and culturing different cells and tissues, etc., the inside of the three-gas culture device and the shaker, etc. need to be fully cleaned and sterilized to avoid affecting the experimental results. The shaker has many structures and is not easy to clean, which can easily cause cross-infection and affect the experimental results. In addition, the oxygen, carbon dioxide and nitrogen used in the three-gas culture device are usually transported into the device through separate pipelines, and one of the gases needs to be added separately according to the actual needs, which causes uneven mixing of the three gases. In addition, when adding gas to the three-gas culture device during the culture process, the pressure difference between the culture bottle and the three-gas culture device is small, which makes it difficult for the gas to enter the culture bottle, affecting the experimental results. There are certain deficiencies. To solve the above problems, an intelligent three-gas culture device with micro-pressure maintaining function is proposed. SUMMARY

[0006] To achieve the above purpose, the present application realizes the following technical scheme: an intelligent three-gas culture device with micro-pressure maintaining function, comprising a three-gas culture box, a tray and a culture bottle, further comprising a blocking mechanism arranged at the lower end of the inner wall of the three-gas culture box, a shaker mechanism arranged at the bottom of the inner wall of the three-gas culture box and an auxiliary mechanism arranged at the top of the inner wall of the three-gas culture box, the blocking mechanism comprising:

[0007] The first sealing plate is fixedly arranged at the lower end of the inner wall of the three-gas incubator, and the lower end of the inner wall of the three-gas incubator and the top of the first sealing plate are fixedly arranged with the second sealing plate, the front of the first sealing plate and the second sealing plate is fixedly arranged with the third sealing plate, the two sides and the bottom of the third sealing plate are fixedly connected with the two sides of the inner wall and the bottom of the inner wall of the three-gas incubator, so as to cooperate with the second sealing plate to block the inside of the three-gas incubator, the first sealing plate and the second sealing plate are arranged in parallel, and the top of the second sealing plate is provided with a gas guide mechanism, and the shaking table mechanism comprises:

[0008] The mounting plate is fixedly arranged at the top of the second sealing plate, and the top of the mounting plate is fixedly arranged with a rubber ring arranged in an annular array, and the top of the first sealing plate is provided with a shaking table assembly arranged in an annular array.

[0009] Further, the shaking table assembly comprises:

[0010] The base is fixedly arranged at the top of the first sealing plate, and the inner wall of the base is rotatably arranged with a threaded rod, and the side wall of the threaded rod is threadedly connected with a threaded tube, and the outer wall of the threaded tube is slidably connected with the inner side wall of the base;

[0011] The rotating sleeve is rotatably arranged at the top of the base, and the top of the rotating sleeve is fixedly arranged with a shaking plate, and the bottom of the shaking plate is provided with a first groove, and the top of the threaded tube extends into the first groove along the inner wall of the rotating sleeve;

[0012] The rocker is movably arranged on the side of the shaking plate away from the threaded tube and extends to the top of the mounting plate along the inner wall of the rubber ring, the side wall of the rocker is sealingly designed with the inner wall of the rubber ring, and the top of the rocker is fixedly arranged with a fixing piece for fixing the tray.

[0013] Further, the shaking table assembly further comprises:

[0014] The second groove is arranged in the inner side wall of the first groove, the inner wall of the second groove is sleeved with a first abutting rod, one end of the first abutting rod extends into the first groove, and the first abutting rod and the threaded tube are designed in an arc shape and a conical shape at one end in the first groove respectively, so as to adjust the horizontal position of the first abutting rod through the inclined surface of the top of the threaded tube, and the first abutting rod can rotate around the inclined surface of the threaded tube;

[0015] The second abutting rod is sleeved in the interior of the shaking plate and located at the top and the bottom of the first abutting rod, and the side of the second abutting rod close to each other is provided with a first tooth groove arranged at intervals, and the top and the bottom of the first abutting rod are provided with a second tooth groove arranged at intervals;

[0016] The transmission gear is rotatably arranged in the interior of the shaking plate and located between the first abutting rod and the second abutting rod, so as to mesh with the first tooth groove and the second tooth groove, so that the moving directions of the first abutting rod and the second abutting rod are opposite.

[0017] Further, the rocker assembly further comprises:

[0018] The first piston block is fixedly arranged at one end of the first abutting rod in the second groove.

[0019] The piston cavity is arranged in the interior of the rocker plate, the inner wall of the piston cavity is sleeved with the second piston block, the inner wall of the piston cavity is fixedly arranged with the conduit, the other end of the conduit extends into the second groove, the side walls of the first piston block and the second piston block are respectively in dynamic sealing design with the inner wall of the second groove and the inner wall of the piston cavity, and the end of the second piston block away from the conduit is fixedly arranged with the guide rod extending into the rocker bed to limit the movement of the second piston block.

[0020] The limiting block is fixedly arranged at the top and bottom of the inner wall of the second groove to limit the position of the first abutting rod.

[0021] Further, the rocker mechanism further comprises:

[0022] The first servo motor is fixedly arranged at the bottom of the inner wall of the three-gas incubator, the second servo motor is fixedly arranged at the top of the inner wall of the three-gas incubator, the output shafts of the first servo motor and the second servo motor are respectively provided with the first transmission mechanism and the second transmission mechanism, so that the first servo motor and the second servo motor drive the rotating sleeve and the threaded rod to rotate through the first transmission mechanism and the second transmission mechanism respectively.

[0023] Further, the gas guide mechanism comprises:

[0024] The first flow guide grid is fixedly arranged at the top of the second sealing plate, the second flow guide grid is fixedly arranged at the top of the second sealing plate, the first flow guide grid and the second flow guide grid are respectively arranged at the two sides of the mounting plate, the first flow guide box and the second flow guide box are respectively fixedly arranged at the two sides of the top of the first sealing plate, and the first flow guide box and the second flow guide box are respectively connected with the first flow guide grid and the second flow guide grid.

[0025] The pump body is fixedly arranged at the bottom of the inner wall of the three-gas incubator, the gas inlet end of the pump body is fixedly arranged with the first gas guide pipe, and the first gas guide pipe is connected with the first flow guide box.

[0026] Further, the gas guide mechanism further comprises:

[0027] The gas mixing device is arranged at the bottom of the inner wall of the three-gas incubator, the gas inlet end of the gas mixing device is connected with the gas outlet end of the second gas guide pipe, the gas outlet end of the gas mixing device is fixedly arranged with the third gas guide pipe, and the third gas guide pipe is connected with the second flow guide box.

[0028] The fourth air guide pipe is fixedly arranged on the outer wall of the third air guide pipe and extends to the top of the second sealing plate. The outer wall of the third air guide pipe is fixedly arranged with a fifth air guide pipe. The outer wall of the end of the third air guide pipe close to the second air guide tank, the outer wall of the fourth air guide pipe and the outer wall of the fifth air guide pipe are all provided with valve bodies.

[0029] Further, the air guide mechanism further comprises a negative pressure assembly arranged on the top of the tray, and the negative pressure assembly comprises:

[0030] The sixth air guide pipe is fixedly arranged on the top of the tray and located outside the culture bottle respectively. The top of the sixth air guide pipe is designed in a bent shape so as to be directed towards the first air guide tank.

[0031] The seventh air guide pipe is fixedly arranged in the interior of the tray and connected with the sixth air guide pipe. The air inlet end of the seventh air guide pipe extends to the outside of the tray to be connected with the fourth air guide pipe.

[0032] Further, the auxiliary mechanism comprises:

[0033] The top seat is fixedly arranged on the inner wall of the top of the three-gas culture tank. The interior of the top seat is provided with auxiliary assemblies arranged in an annular array. The auxiliary assemblies comprise:

[0034] The electric push rod is fixedly arranged in the interior of the top seat and extends to the bottom of the top seat. The telescopic shaft of the electric push rod is fixedly arranged with a first connecting block. The bottom and the side wall of the first connecting block are fixedly arranged with a first conductive block and an electric connector respectively. The first conductive block and the electric connector are connected through wires.

[0035] The second connecting block is detachably arranged on the side wall of the first connecting block. The top of the second connecting block is provided with a third groove. The inner wall of the third groove is fixedly arranged with a second conductive block.

[0036] The rubber plug is fixedly arranged on the bottom of the second connecting block. The bottom of the rubber plug is fixedly arranged with a pressure relief pipe extending to the upper end of the side wall of the rubber plug. The outer wall of one end of the pressure relief pipe located at the upper end of the side wall of the rubber plug is provided with a pressure relief valve. The bottom of the rubber plug is fixedly arranged with a pressure sensor. The pressure sensor and the pressure relief valve are both connected with the second conductive block through wires.

[0037] Further, the auxiliary assembly further comprises:

[0038] The annular groove is arranged on the side wall of the first connecting block.

[0039] The arc-shaped plate is movably arranged on the top of the second connecting block and symmetrically arranged with the center point of the second connecting block.

[0040] The moving piece is sleeved on the inner upper end of the second connecting block and extends to the top of the second connecting block, the top of the moving piece is fixedly connected with the bottom of the arc-shaped plate, the inner portion of the second connecting block is sleeved with a spring for elastically supporting the moving piece, and the top of the second connecting block is provided with a moving groove for the movement of the moving piece and the expansion and contraction of the spring.

[0041] The application provides a smart three-gas culture device with micro-pressure maintaining function.

[0042] 1、The culture bottle and the cell are separated from the shaking bed mechanism and the gas guiding mechanism, so that the difficulty of cleaning the inside of the three-gas culture box is reduced, cross infection is reduced, the culture of different cells and tissues is facilitated, and the shaking of the tray and the culture bottle driven by the shaking bed mechanism is not affected.

[0043] 2、The culture bottle is shaken by the shaking bed mechanism, and the shaking amplitude is adjusted, so that the shaking of the culture solution is accelerated, the oxygen is uniformly distributed, the metabolic demand of aerobic cells is met, the nutrients in the culture medium are uniformly diffused, the metabolic waste is discharged, the growth of cells is not inhibited by the local high concentration, and the dynamic oscillation is closer to the in-vivo environment, which promotes cell metabolism and division, and is especially suitable for tumor cell or stem cell research.

[0044] The shaking amplitude of the culture bottle of the tray is adjusted to enhance the mixing effect, make the dissolved oxygen content in the culture solution more uniform, and adapt to different needs: when low-oxygen culture is performed, the shaking amplitude is appropriately reduced to reduce gas disturbance, and when high-oxygen culture is performed, the shaking amplitude is increased to strengthen oxygen diffusion.

[0045] 3、The first resistance rod is arranged on the inclined surface of the threaded pipe, the shaking amplitude of the rocker is adjusted, the rotation of the rocker is not affected, the moving directions of the first resistance rod and the second resistance rod are opposite, the stability of the adjustment and shaking of the rocker is not affected by the centrifugal force during the rotation of the rocker, and the stability of the first resistance rod is improved under the extrusion of the gas pressure and the inclined surface.

[0046] The rocking bed mechanism is used to drive the rocking rod to rotate synchronously, the positions of the multiple rocking rods are adjusted synchronously, the stability of the rocking of the rocking bed is improved, and the synchronization of the adjustment is improved.

[0047] 4、The present application makes the gas in the three gas incubator circulate and flow through the gas guide mechanism, so that the gas is evenly distributed in the three gas incubator, ensuring that the culture is in the same condition, avoiding uneven cell growth or abnormal metabolism caused by local concentration difference;

[0048] By extracting the gas in the three gas incubator and opening the door, subsequent processing such as gas filtration can be recycled, reducing gas waste. Cooperating with the auxiliary mechanism does not affect the culture of cells and the like;

[0049] The negative pressure assembly forms negative pressure at the bottle opening of the culture bottle, so as to quickly replace the gas in the culture bottle with the gas in the three gas incubator, thereby facilitating the culture of cells.

[0050] 5、The present application seals the bottle opening of the culture bottle through the auxiliary mechanism, so that the culture bottle contains a gas environment that meets the survival needs of cells for a short time, avoiding rapid leakage of the gas inside the three gas incubator and the culture bottle when the culture bottle is taken out, resulting in rapid increase of the pressure difference between the inside and outside of the three gas incubator and the culture bottle, thereby avoiding damage to the cells during this process, and avoiding the entry of external gas into the three gas incubator;

[0051] The pressure sensor, pressure relief pipe and pressure relief valve facilitate the balance of the pressure inside and outside the culture bottle, avoiding damage to the cells in the culture bottle during plugging, and the first conductive block facilitates the power supply of the pressure sensor and the pressure relief valve, without affecting the sealing plug with the culture bottle taken out of the three gas incubator. BRIEF DESCRIPTION OF DRAWINGS

[0052] Figure 1 It is a whole side structure schematic diagram of the present application;

[0053] Figure 2 It is a whole front structure schematic diagram of the present application;

[0054] Figure 3 It is a longitudinal section structure schematic diagram of part of the three gas incubator of the present application;

[0055] Figure 4 It is a structure schematic diagram of the blocking mechanism, the shaking table mechanism, the tray and the gas guide mechanism of the present application;

[0056] Figure 5 It is a structure schematic diagram of the shaking table mechanism of the present application;

[0057] Figure 6 It is a structure schematic diagram of the first sealing plate, the second sealing plate and the tray of the present application;

[0058] Figure 7 It is a structure schematic diagram of the first sealing plate, the second sealing plate and the tray of the present application;

[0059] Figure 8 Structure diagram of the rocking bed assembly, the first transmission mechanism, the second transmission mechanism, the first servo motor and the second servo motor of the present application;

[0060] Figure 9 Structure diagram of the rocking bed assembly of the present application;

[0061] Figure 10 Structure diagram of the rocking plate and the base in longitudinal section of the present application;

[0062] Figure 11 Structure diagram of the rocking plate, the rotating sleeve and the threaded tube in longitudinal section of the present application;

[0063] Figure 12 Structure diagram of the rocking plate in horizontal and longitudinal section of the present application;

[0064] Figure 13 Structure diagram of one side of the air guiding mechanism of the present application;

[0065] Figure 14 Structure diagram of the other side of the air guiding mechanism of the present application;

[0066] Figure 15 Structure diagram of the tray, the negative pressure assembly and the culture bottle of the present application;

[0067] Figure 16 Structure diagram of the tray in horizontal section of the present application;

[0068] Figure 17 Structure diagram of the auxiliary mechanism of the present application;

[0069] Figure 18 Structure diagram of the top base in longitudinal section of the present application;

[0070] Figure 19 Structure diagram of the pressure sensor, the pressure relief tube, the pressure relief valve and the rubber plug of the present application;

[0071] Figure 20 Structure diagram of the second connecting block in longitudinal section of the present application;

[0072] Figure 21 Structure diagram of the electric connector, the first conductive block, the second conductive block, the third recess and the annular recess of the present application.

[0073] Reference signs involved in the above-mentioned drawings: 1, three-gas incubator; 2, auxiliary mechanism; 3, culture bottle; 4, tray; 5, blocking mechanism; 6, rocking bed mechanism; 7, air guiding mechanism;

[0074] 21, top base; 22, auxiliary assembly;

[0075] 220, electric connector; 221, electric push rod; 222, second connecting block; 223, first connecting block; 224, rubber plug; 225, pressure relief pipe; 226, pressure sensor; 227, pressure relief valve; 228, moving piece; 229, arc plate; 2291, second conductive block; 2292, annular groove; 2293, first conductive block; 2294, third groove;

[0076] 51, third sealing plate; 52, second sealing plate; 53, first sealing plate;

[0077] 61, mounting plate; 62, rubber ring; 63, cradle assembly; 64, first transmission mechanism; 65, first servo motor; 66, second transmission mechanism; 67, second servo motor;

[0078] 630, piston cavity; 631, fixing piece; 632, rocking plate; 633, base; 634, rocker; 635, second abutting rod; 636, second groove; 637, first abutting rod; 638, threaded pipe; 639, rotating sleeve; 6391, inclined surface; 6392, first groove; 6393, threaded rod; 6394, first tooth groove; 6395, first piston block; 6396, limiting block; 6397, second tooth groove; 6398, transmission gear; 6399, guide pipe; 6390, second piston block;

[0079] 71, gas mixing device; 72, third gas guide pipe; 73, second flow guide box; 74, second flow guide grid; 75, fourth gas guide pipe; 76, first flow guide grid; 77, first flow guide box; 78, pump body; 79, first gas guide pipe; 791, second gas guide pipe; 792, fifth gas guide pipe; 793, negative pressure assembly;

[0080] 7931, sixth gas guide pipe; 7932, seventh gas guide pipe. DETAILED DESCRIPTION

[0081] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0082] Embodiment one: please refer to Figure 1 , Figure 2 and Figure 3The utility model provides a kind of intelligent three gas culture device with micro-pressure maintaining function, including three gas incubator 1, tray 4 and culture bottle 3, also including the blocking mechanism 5 being arranged in the lower end of the inner wall of three gas incubator 1, the shaking table mechanism 6 being arranged in the bottom of the inner wall of three gas incubator 1 and the auxiliary mechanism 2 being arranged in the top of the inner wall of three gas incubator 1.

[0083] When the utility model is implemented, the box door of three gas incubator 1 is opened, culture bottle 3 containing cells or tissues etc. is fixed with tray 4, then, tray 4 is fixedly connected with the fixing piece 631 of shaking table assembly 63, so as to place culture bottle 3 in three gas incubator 1, and subsequent culture bottle 3 is driven to shake by shaking table mechanism 6, to assist the culture of cells and tissues etc. in culture bottle 3, so as to facilitate experimental use, after culture bottle 3 is placed in three gas incubator 1, the box door is closed, and the external interface of three gas incubator 1 is connected with the external pipeline of oxygen, carbon dioxide and nitrogen, so as to transport oxygen, carbon dioxide and nitrogen into three gas incubator 1, to make three gas incubator 1 contain oxygen, carbon dioxide and nitrogen content suitable for culture cells, and a certain gas pressure environment can be maintained in three gas incubator 1, so as to facilitate the culture of cells and tissues etc.

[0084] During the culture process, when shaking table is needed to assist the culture of culture cells, the first servo motor 65 and the first transmission mechanism 64 of shaking table mechanism 6 drive the annular array arrangement of shaking table assembly 63 to work, so as to drive the rotation of rocker 634 and shaking plate 632 around the center point of rotating sleeve 639, to drive tray 4 and culture bottle 3 to shake, to assist the culture of cells etc. in culture bottle 3, and the second servo motor 67 and the second transmission mechanism 66 can synchronously adjust the position of the annular array arrangement of rocker 634, to adjust the amplitude of the shaking of tray 4, to facilitate the adjustment according to the culture demand of cells, to be suitable for more culture demands.

[0085] Secondly, the circulation of oxygen, carbon dioxide and nitrogen etc. gas in three gas incubator 1 is realized by gas guide assembly, to facilitate the uniform mixing of gas, to avoid the uneven growth of cells or abnormal metabolism caused by local concentration difference, when one or more kinds of gas are added into three gas incubator 1, negative pressure is formed at the bottle mouth of culture bottle 3 by negative pressure assembly 793, to facilitate the outflow of gas in culture bottle 3, to facilitate the replacement of gas in three gas incubator 1 with the gas in culture bottle 3, to facilitate the addition of gas into culture bottle 3, to avoid the low speed of gas into culture bottle 3 caused by the small gas pressure difference between culture bottle 3 and three gas incubator 1, to avoid the influence on culture use, and the structure of gas guide mechanism 7 and shaking table mechanism 6 is blocked inside the second sealing plate 52 and the third sealing plate 51 by blocking mechanism 5, to avoid a large amount of structure and cells in the same environment, to reduce the difficulty of cleaning and cross infection when different cells etc. are cultured.

[0086] After the culture is completed, the bottle mouth of the culture bottle 3 is sealed by the auxiliary mechanism 2, so that the required gas pressure environment in the culture bottle 3 is maintained for a short time, so as to reduce the damage to the cells during the process of taking out the culture bottle 3 and the cells from the three-gas incubator 1, and avoid the entry of external gas and bacteria into the culture bottle 3, thereby avoiding the pollution of the cells.

[0087] Please refer to Figure 3 and Figure 4 , the blocking mechanism 5 comprises:

[0088] The first sealing plate 53 is fixedly arranged at the lower end of the inner wall of the three-gas incubator 1, and the top of the first sealing plate 53 is fixedly arranged with the second sealing plate 52. The front surface of the first sealing plate 53 and the second sealing plate 52 is fixedly arranged with the third sealing plate 51. The two sides and the bottom of the third sealing plate 51 are fixedly connected with the two side inner walls and the bottom inner wall of the three-gas incubator 1, respectively, so as to cooperate with the second sealing plate 52 to block the inside of the three-gas incubator 1. The third sealing plate 51 is arranged vertically with the first sealing plate 53, and the first sealing plate 53 is arranged in parallel with the second sealing plate 52. The top of the second sealing plate 52 is provided with the gas guiding mechanism 7.

[0089] In specific implementation, the second sealing plate 52 and the third sealing plate 51 form an L-shaped structure to block the inside of the three-gas incubator 1, so that the inside of the three-gas incubator 1 is divided into two independent and sealed spaces, so that the culture bottle 3 and the cells are separated from the shaking bed mechanism 6 and the gas guiding mechanism 7, etc. A large number of structures are avoided to be in the same environment with the cells. When different cells are cultured, the difficulty of cleaning the inside of the three-gas incubator 1 is reduced, and cross infection is reduced, so that the culture of different cells and tissues is facilitated, and the shaking of the shaking bed mechanism 6 driving the tray 4 and the culture bottle 3 is not affected. In addition, the gas in the three-gas incubator 1 is circulated and flows, so that the gas in the three-gas incubator 1 is uniformly distributed, so as to ensure that all the cultures are in the same condition, and avoid the uneven growth or abnormal metabolism of the cells caused by the local concentration difference.

[0090] The gap is left between the first sealing plate 53 and the second sealing plate 52, so as to facilitate the installation of the shaking bed mechanism 6 and the gas guiding mechanism 7, etc. The first sealing plate 53, the second sealing plate 52 and the third sealing plate 51 are all detachable sealing plate structures, so as to facilitate the subsequent maintenance of the shaking bed mechanism 6 and the gas guiding mechanism 7, etc.

[0091] Please refer to Figure 5 , Figure 6 , Figure 7 , Figure 8 , Figure 9 , Figure 10 , Figure 11 and Figure 12 , the shaking bed mechanism 6 comprises:

[0092] The mounting plate 61 is fixedly arranged on the top of the second sealing plate 52, and an annular array of rubber rings 62 is arranged on the top of the mounting plate 61. The top of the first sealing plate 53 is provided with an annular array of rocking bed assemblies 63.

[0093] The rocking bed assembly 63 comprises:

[0094] The base 633 is fixedly arranged on the top of the first sealing plate 53, and a threaded rod 6393 is rotatably arranged on the inner wall of the base 633. A threaded tube 638 is threadedly connected to the side wall of the threaded rod 6393, and the outer wall of the threaded tube 638 is slidably connected to the inner side wall of the base 633.

[0095] The rotating sleeve 639 is rotatably arranged on the top of the base 633, and a rocking plate 632 is fixedly arranged on the top of the rotating sleeve 639. The bottom of the rocking plate 632 is provided with a first recess 6392, and the top of the threaded tube 638 extends into the first recess 6392 along the inner wall of the rotating sleeve 639.

[0096] The rocking lever 634 is movably arranged on the side of the rocking plate 632 away from the threaded tube 638, and extends to the top of the mounting plate 61 along the inner wall of the rubber ring 62. The side wall of the rocking lever 634 is sealingly designed with the inner wall of the rubber ring 62, and a fixing member 631 is fixedly arranged on the top of the rocking lever 634 to fix the tray 4.

[0097] The rocking bed assembly 63 further comprises:

[0098] The second recess 636 is arranged on the inner side wall of the first recess 6392, and the first abutting rod 637 is sleeved on the inner wall of the second recess 636. One end of the first abutting rod 637 extends into the first recess 6392. The first abutting rod 637 and the threaded tube 638 are arc-shaped and conical-shaped, respectively, at the end located in the first recess 6392, so as to adjust the horizontal position of the first abutting rod 637 through the inclined surface 6391 at the top of the threaded tube 638, and enable the first abutting rod 637 to rotate around the inclined surface 6391 of the threaded tube 638.

[0099] The second abutting rod 635 is sleeved on the inside of the rocking plate 632 and located at the top and bottom of the first abutting rod 637. The side of the second abutting rod 635 close to each other is provided with a first tooth groove 6394 arranged at intervals, and the top and bottom of the first abutting rod 637 are provided with a second tooth groove 6397 arranged at intervals.

[0100] The transmission gear 6398 is rotatably arranged on the inside of the rocking plate 632 and located between the first abutting rod 637 and the second abutting rod 635, so as to be engaged with the first tooth groove 6394 and the second tooth groove 6397, thereby making the moving directions of the first abutting rod 637 and the second abutting rod 635 opposite.

[0101] The rocking bed assembly 63 further comprises:

[0102] The first piston block 6395 is fixedly arranged at one end of the first abutting rod 637 in the second groove 636.

[0103] The piston cavity 630 is arranged in the interior of the rocker plate 632, the inner wall of the piston cavity 630 is sleeved with the second piston block 6390, the inner wall of the piston cavity 630 is fixedly arranged with the conduit 6399, the other end of the conduit 6399 extends into the second groove 636, the side wall of the first piston block 6395 and the second piston block 6390 is respectively in dynamic sealing design with the inner wall of the second groove 636 and the piston cavity 630, the end of the second piston block 6390 away from the conduit 6399 is fixedly arranged with a guide rod extending into the rocking bed to limit the movement of the second piston block 6390.

[0104] The limiting block 6396 is fixedly arranged at the top and bottom of the inner wall of the second groove 636 to limit the position of the first abutting rod 637.

[0105] The rocking bed mechanism 6 further comprises:

[0106] The first servo motor 65 is fixedly arranged at the bottom of the inner wall of the three-gas incubator 1, the second servo motor 67 is fixedly arranged at the top of the inner wall of the three-gas incubator 1, the output shafts of the first servo motor 65 and the second servo motor 67 are respectively provided with the first transmission mechanism 64 and the second transmission mechanism 66, so that the first servo motor 65 and the second servo motor 67 drive the rotating sleeve 639 and the threaded rod 6393 to rotate through the first transmission mechanism 64 and the second transmission mechanism 66.

[0107] In specific implementation, when it is needed to shake the tray 4 and the culture bottle 3, the first servo motor 65 is started, the first servo motor 65 drives the rotating sleeve 639 to rotate through the first transmission mechanism 64, the rotating sleeve 639 drives the rocker plate 632, the second abutting rod 635 and the rocker lever 634 to rotate along the center point of the rotating sleeve 639, so as to drive the tray 4 and the culture bottle 3 to shake through the fixing piece 631, the culture solution is shaken to accelerate the dissolution and uniform distribution of oxygen, to meet the metabolic demand of aerobic cells, the oscillation makes the nutrients in the culture medium diffuse more uniformly and accelerates the discharge of metabolic waste, to avoid local high concentration to inhibit the growth of cells, in addition, the dynamic oscillation is closer to the in-vivo environment of organisms, to promote cell metabolism and division, which is especially suitable for tumor cell or stem cell research.

[0108] To meet the shaking needs of different cells, the threaded rod 6393 is driven to rotate by the second servo motor 67 and the second transmission mechanism 66, the threaded rod 6393 drives the threaded tube 638 to move vertically, so as to press the first stop rod 637 horizontally through the slope 6391 at the top of the threaded tube 638, thereby driving the second stop rod 635 and the rocker 634 to move horizontally, so as to adjust the distance between the rocker 634 and the rotating sleeve 639, thereby adjusting the amplitude of the culture bottle 3 of the tray 4 to enhance the mixing effect, so that the dissolved oxygen content in the culture solution is more uniform, and different needs are met: when low-oxygen culture, appropriately reducing the amplitude of shaking can reduce gas disturbance, and when high-oxygen culture, increasing the amplitude of shaking can strengthen oxygen diffusion.

[0109] When the threaded tube 638 is driven to move vertically by the second servo motor 67, so as to adjust the horizontal position of the first stop rod 637 through the slope 6391, the slope 6391 presses the first stop rod 637 to move, at the same time, the second stop rod 635 is driven to move in the opposite direction of the first stop rod 637 through the meshing of the second tooth groove 6397 and the first tooth groove 6394 with the transmission gear 6398, so as to adjust the position of the rocker 634 to adjust the amplitude of shaking, in this process, since the rocker 634 needs to rotate along the center point of the rotating sleeve 639 to meet the shaking needs, so that the arc-shaped part of the first stop rod 637 rotates along the slope 6391 of the threaded tube 638, facilitating the adjustment of the shaking amplitude of the rocker 634 without affecting the rotation of the rocker 634, and the moving directions of the first stop rod 637 and the second stop rod 635 are opposite, in the process of rotation of the rocker 634, under the action of centrifugal force, the rocker 634 and the second stop rod 635 move away from the threaded tube 638, thereby driving the first stop rod 637 to move closer to the threaded tube 638, so as to abut against the threaded tube 638, avoiding the influence of centrifugal force on the adjustment and shaking stability of the rocker 634 when rotating, and when the threaded tube 638 presses the first stop rod 637 closer to the rocker 634 through the slope 6391, the first stop rod 637 drives the first piston block 6395 to press the gas in the second recess 636 to flow into the piston cavity 630 along the conduit 6399, thereby pressing the second piston block 6390 and the guide rod to move, so that the first stop rod 637 remains in a stable state under the pressing action of the gas pressure and the slope 6391, thereby improving the stability, and the first piston block 6395 is stopped by the limiting block 6396, thereby limiting the movement of the first stop rod 637.

[0110] In order to ensure the stability of the shaking of the tray 4 and the culture bottle 3, the tray 4 is usually driven to shake by the synchronous rotation of the plurality of rocking rods 634, and for the convenience of synchronous adjustment and synchronous driving of the rotation of the rocking rods 634, the first transmission mechanism 64 comprises a first gear fixedly sleeved on the outer side of the rotating sleeve 639, a first rotating shaft rotatably arranged on the bottom of the inner wall of the three-gas incubator 1, a second gear fixedly sleeved on the upper end of the side wall of the first rotating shaft, and a first chain for transmission connection between the first gear and the second gear. The bottom of the first rotating shaft is in transmission connection with the output shaft of the first servo motor 65 through a bevel gear, so as to synchronously drive the rotation of the annular array arranged rotating sleeve 639, so as to synchronously drive the plurality of rocking rods 634 to shake, and the stability of the shaking of the tray 4 is improved. For the convenience of synchronous adjustment of the position of the rocking rod 634, the second transmission mechanism 66 comprises a third gear fixedly sleeved on the side wall of the threaded rod 6393, a second rotating shaft rotatably arranged on the bottom of the inner wall of the three-gas incubator 1, a fourth gear fixedly sleeved on the upper end of the side wall of the second rotating shaft, and a second chain for transmission connection between the third gear and the fourth gear. The second rotating shaft is connected with the output shaft of the second servo motor 67 through a bevel gear, so as to facilitate the synchronous adjustment of the position of the rocking rod 634, and the adjustment is convenient.

[0111] When the rocking rod 634 rotates, the rubber ring 62 seals between the rocking rod 634 and the mounting plate 61. Since the rubber ring 62 has a certain elasticity, it does not affect the rotation of the rocking rod 634 while sealing between the rocking rod 634 and the mounting plate 61, and does not affect the adjustment of the position of the rocking rod 634. The top of the mounting plate 61 is provided with a through hole for mounting a sealing ring, and there is space for adjusting the rocking rod 634, so as to avoid affecting the rotation and adjustment of the rocking rod 634.

[0112] By only making part of the rocking rod 634 and the fixing piece 631 of the rocking bed mechanism 6 and the culture bottle 3 in the same environment, subsequent cleaning and use are facilitated.

[0113] When the culture bottle 3 and the cells are shaken, a filter membrane is usually installed on the inner side of the bottle mouth before the culture bottle 3 is placed in the three-gas incubator 1, so as to avoid the leakage of the culture solution while not affecting the entry of the gas into the culture bottle 3.

[0114] Embodiment two: please refer to Figure 13 and Figure 14 The difference between the technical scheme of the embodiment and the embodiment one is that the gas guiding mechanism 7 comprises:

[0115] A first flow guide grid 76 is fixedly arranged on the top of the second sealing plate 52, a second flow guide grid 74 is fixedly arranged on the top of the second sealing plate 52, the first flow guide grid 76 and the second flow guide grid 74 are respectively arranged on the two sides of the mounting plate 61, a first flow guide box 77 and a second flow guide box 73 are respectively fixedly arranged on the top of the first sealing plate 53, and the first flow guide box 77 and the second flow guide box 73 are respectively connected with the first flow guide grid 76 and the second flow guide grid 74.

[0116] A pump body 78 is fixedly arranged at the bottom of the inner wall of the three-gas incubator 1. A first gas guide pipe 79 is fixedly arranged at the gas inlet end of the pump body 78. The first gas guide pipe 79 is connected with the first flow guide box 77. A second gas guide pipe 791 is fixedly arranged at the gas outlet end of the pump body 78.

[0117] The gas guide mechanism 7 further comprises:

[0118] A gas mixing device 71 is arranged at the bottom of the inner wall of the three-gas incubator 1. The gas inlet end of the gas mixing device 71 is connected with the gas outlet end of the second gas guide pipe 791. A third gas guide pipe 72 is fixedly arranged at the gas outlet end of the gas mixing device 71. The third gas guide pipe 72 is connected with the second flow guide box 73.

[0119] A fourth gas guide pipe 75 is fixedly arranged at the outer wall of the third gas guide pipe 72 and extends to the top of the second sealing plate 52. A fifth gas guide pipe 792 is fixedly arranged at the outer wall of the third gas guide pipe 72. The outer wall of the end of the third gas guide pipe 72 close to the second flow guide box, the outer wall of the fourth gas guide pipe 75 and the outer wall of the fifth gas guide pipe 792 are all provided with valve bodies.

[0120] In the specific implementation, the gas in the three-gas incubator 1 is drawn into the pump body 78 through the gas inlet end of the pump body 78 along the first flow guide grid 76, the first flow guide box and the first gas guide pipe 79. The gas is discharged into the three-gas incubator 1 again through the gas outlet end of the pump body 78, the second gas guide pipe 791, the gas mixing device 71, the third gas guide pipe 72, the second flow guide box and the second flow guide grid, so that the gas circulates in the three-gas incubator 1, the gas is uniformly distributed in the three-gas incubator 1, the culture is ensured to be in the same condition, and the cell growth is not uniform or the metabolism is abnormal due to local concentration difference. In the circulation process, the uniformity of the gas mixing is further improved through the gas mixing device 71, and the use of the culture is not affected.

[0121] When the culture is completed, before the door of the incubator is opened and before the bottle opening is sealed by the auxiliary mechanism 2, the fifth gas guide pipe 792 is connected with a pipeline, so that the pump body 78 draws out the oxygen, carbon dioxide and nitrogen in the three-gas incubator 1, thereby facilitating the subsequent recycling of the gas filtering treatment, reducing the waste of the gas, and generally containing a certain concentration of oxygen, thereby facilitating the recycling of the gas resources, and cooperating with the auxiliary mechanism 2, without affecting the culture of the cells.

[0122] Only the first flow guide grid 76 and the second flow guide grid 74 are in the same environment as the culture bottle 3, thereby facilitating the actual cleaning and use.

[0123] The gas mixing device 71 can adopt a mixed gas proportioner and an ISG type static mixer. This is prior art and will not be described here.

[0124] Please refer toFigure 15 and Figure 16 , the air guide mechanism 7 further comprises a negative pressure assembly 793 arranged on the top of the tray 4, the negative pressure assembly 793 comprises:

[0125] a sixth air guide pipe 7931 fixedly arranged on the top of the tray 4 and located outside the culture bottle 3 respectively, the top of the sixth air guide pipe 7931 is designed in a bent shape, so that the top of the sixth air guide pipe 7931 is directed towards the first air guide box;

[0126] a seventh air guide pipe 7932 fixedly arranged inside the tray 4 and connected with the sixth air guide pipe 7931, the air inlet end of the seventh air guide pipe 7932 extends to the outside of the tray 4 to be connected with the fourth air guide pipe 75.

[0127] During the culture process, when it is necessary to add gas into the three-gas incubator 1, in order to facilitate the gas to quickly enter the culture bottle 3 to contact with the culture such as cells, the pump body 78 is used to make the discharged gas enter into the seventh air guide pipe 7932 and the sixth air guide pipe 7931 along the fourth air guide pipe 75, so as to form a negative pressure at the bottle opening of the culture bottle 3, so as to facilitate the replacement of the gas in the culture bottle 3 with the gas in the three-gas incubator 1, thereby facilitating the culture of cells, and in this process, the gas can be mixed, further improving the use effect.

[0128] The fourth air guide pipe 75 and the seventh air guide pipe 7932 are connected by an external pipeline when the tray 4 is installed, the external pipeline adopts a hose, and a length for shaking the tray 4 is reserved, facilitating actual use.

[0129] Embodiment three: please refer to Figure 17 、 Figure 18 、 Figure 19 、 Figure 20 and Figure 21 The difference between the technical scheme of this embodiment and that of embodiment two is that the auxiliary mechanism 2 comprises:

[0130] a top seat 21 fixedly arranged on the top of the inner wall of the three-gas incubator 1, the inside of the top seat 21 is provided with auxiliary assemblies 22 arranged in an annular array, the auxiliary assemblies 22 comprise:

[0131] an electric push rod 221 fixedly arranged inside the top seat 21 and extending to the bottom of the top seat 21, the telescopic shaft of the electric push rod 221 is fixedly provided with a first connecting block 223, the bottom and the side wall of the first connecting block 223 are respectively fixedly provided with a first conductive block 2293 and an electric connection head 220, the first conductive block 2293 and the electric connection head 220 are connected by wires;

[0132] The second connecting block 222 is detachably arranged on the side wall of the first connecting block 223, and the top of the second connecting block 222 is provided with a third groove 2294, and the inner wall bottom of the third groove 2294 is fixedly provided with a second conductive block 2291;

[0133] The rubber plug 224 is fixedly arranged on the bottom of the second connecting block 222, and the bottom of the rubber plug 224 is fixedly provided with a pressure relief pipe 225 extending to the upper end of the side wall of the rubber plug 224, and one end of the pressure relief pipe 225 located at the upper end of the side wall of the rubber plug 224 is provided with a pressure relief valve 227, and the bottom of the rubber plug 224 is fixedly provided with a pressure sensor 226, and the pressure sensor 226 and the pressure relief valve 227 are connected with the second conductive block 2291 through wires.

[0134] The auxiliary assembly 22 further comprises:

[0135] The annular groove 2292 is arranged on the side wall of the first connecting block 223.

[0136] The arc-shaped plate 229 is movably arranged on the top of the second connecting block 222 and symmetrically arranged with the center point of the second connecting block 222.

[0137] The moving part 228 is sleeved on the upper end of the inside of the second connecting block 222 and extends to the top of the second connecting block 222, the top of the moving part 228 is fixedly connected with the bottom of the arc-shaped plate 229, the inside of the second connecting block 222 is sleeved with a spring for elastically supporting the moving part 228, and the top of the second connecting block 222 is provided with a moving groove for the movement of the moving part 228 and the expansion and contraction of the spring.

[0138] In specific implementation, after the culture is completed, the first connecting block 223, the second connecting block 222 and the rubber plug 224 are driven by the electric push rod 221 to move downward, so as to seal the bottle opening of the culture bottle 3, so that when the culture bottle 3 is taken out from the three-gas incubator 1, the culture bottle 3 contains a gas environment required for cell survival for a short time, etc., to avoid the rapid leakage of the gas inside the three-gas incubator 1 and the culture bottle 3 when the culture bottle 3 is taken out, so as to avoid the rapid increase of the pressure difference between the inside and outside of the three-gas incubator 1 and the culture bottle 3, thereby avoiding the damage to the cells in the process and avoiding the entry of external gas into the three-gas incubator 1.

[0139] Since the rubber plug 224 will extrude the gas in the culture bottle 3 when moving downward to block the bottle mouth, the gas in the culture bottle 3 will be too high, therefore, the pressure sensor 226 is used for monitoring, and the pressure relief pipe 225 and the pressure relief valve 227 are used for keeping the pressure balance inside and outside the culture bottle 3, so as to avoid damaging the cells in the culture bottle 3 when blocking, and after the blocking is completed, the arc-shaped plates 229 are away from each other, so that the arc-shaped plates 229 are separated from the annular grooves 2292, thereby separating the second connecting block 222 from the first connecting block 223, so as to block without affecting the rubber plug 224 to communicate the culture bottle 3 to take out of the three-gas incubator 1.

[0140] In order to ensure the power supply of the pressure sensor 226 and the pressure relief valve 227, after the second connecting block 222 and the first connecting block 223 are connected, the first conductive block 2293 and the second conductive block 2291 are in contact with each other, so that the electric connector 220 is connected with the power supply and the controller, so as to facilitate actual control and use, and the second connecting block 222 is taken out of the three-gas incubator 1 with the culture bottle 3, which is convenient for subsequent cleaning and use.

[0141] Meanwhile, the contents not described in detail in the specification all belong to the prior art known by those skilled in the art.

[0142] It should be noted that in this paper, relationship terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between the entities or operations. Moreover, the term "includes", "contains" or any other variant thereof is intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment.

[0143] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An intelligent three-gas culture device with micro-pressure maintaining function, comprising a three-gas culture box, a tray and a culture bottle, characterized in that, The sealing mechanism is arranged at the lower end of the inner wall of the three-gas incubator, the shaking table mechanism is arranged at the bottom of the inner wall of the three-gas incubator, and the auxiliary mechanism is arranged at the top of the inner wall of the three-gas incubator. The first sealing plate is fixedly arranged at the lower end of the inner wall of the three-gas incubator, the second sealing plate is fixedly arranged at the lower end of the inner wall of the three-gas incubator and located at the top of the first sealing plate, the front surface of the first sealing plate and the second sealing plate is fixedly arranged with the third sealing plate, the two sides and the bottom of the third sealing plate are fixedly connected with the two side inner walls and the bottom inner wall of the three-gas incubator respectively, so as to cooperate with the second sealing plate to seal the inside of the three-gas incubator, the third sealing plate is arranged vertically with the first sealing plate, the first sealing plate is arranged in parallel with the second sealing plate, the top of the second sealing plate is provided with a gas guide mechanism, the shaking table mechanism comprises: The mounting plate is fixedly arranged at the top of the second sealing plate, the top of the mounting plate is fixedly arranged with a rubber ring arranged in a ring array, and the top of the first sealing plate is provided with a shaking table assembly arranged in a ring array. The shaking table assembly comprises: The base is fixedly arranged at the top of the first sealing plate, the inner wall of the base is rotatably arranged with a threaded rod, the side wall of the threaded rod is threadedly connected with a threaded tube, and the outer wall of the threaded tube is slidably connected with the inner side wall of the base. The rotating sleeve is rotatably arranged at the top of the base, the top of the rotating sleeve is fixedly arranged with a shaking plate, the bottom of the shaking plate is provided with a first groove, and the top of the threaded tube extends into the first groove along the inner wall of the rotating sleeve. The shaking rod is movably arranged on the side of the shaking plate away from the threaded tube and extends to the top of the mounting plate along the inner wall of the rubber ring, the side wall of the shaking rod is sealingly designed with the inner wall of the rubber ring, and the top of the shaking rod is fixedly arranged with a fixing piece for fixing the tray. The shaking table assembly further comprises: The second groove is arranged in the inner side wall of the first groove, the inner wall of the second groove is sleeved with a first abutting rod, one end of the first abutting rod extends into the first groove, and the first abutting rod and the threaded tube are designed in an arc shape and a conical shape respectively at one end in the first groove, so as to adjust the horizontal position of the first abutting rod through the inclined surface at the top of the threaded tube and enable the first abutting rod to rotate around the inclined surface of the threaded tube. The second abutting rod is sleeved in the inside of the shaking plate and located at the top and the bottom of the first abutting rod, and the sides of the second abutting rod close to each other are both provided with first tooth grooves arranged at intervals. The transmission gear is rotatably arranged in the inside of the shaking plate and located between the first abutting rod and the second abutting rod, so as to be engaged with the first tooth grooves and the second tooth grooves, thereby making the moving directions of the first abutting rod and the second abutting rod opposite. The gas guide mechanism further comprises a negative pressure assembly arranged on the top of the tray, and the negative pressure assembly comprises: The sixth gas guide pipe is fixedly arranged on the top of the tray and located outside the culture bottle respectively, and the top of the sixth gas guide pipe is designed in a bent shape, so that the top of the sixth gas guide pipe faces the culture bottle. The auxiliary mechanism comprises: The top base is fixedly arranged at the top of the inner wall of the three-gas incubator, the inside of the top base is provided with auxiliary assemblies arranged in a ring array, and the auxiliary assembly comprises: The electric push rod is fixedly arranged in the inner part of the top base and extends to the bottom of the top base. The telescopic shaft of the electric push rod is fixedly arranged with a first connecting block. The bottom and the side wall of the first connecting block are fixedly arranged with a first conductive block and an electric connector, respectively. The first conductive block and the electric connector are connected through a wire. The second connecting block is detachably arranged on the side wall of the first connecting block. The top of the second connecting block is provided with a third groove. The inner wall bottom of the third groove is fixedly arranged with a second conductive block. The rubber plug is fixedly arranged on the bottom of the second connecting block. The bottom of the rubber plug is fixedly arranged with a pressure relief pipe extending to the upper end of the side wall of the rubber plug. The outer wall of one end of the pressure relief pipe located at the upper end of the side wall of the rubber plug is provided with a pressure relief valve. The bottom of the rubber plug is fixedly arranged with a pressure sensor. The pressure sensor and the pressure relief valve are connected with the second conductive block through wires. 2.The intelligent three-gas culture device with micro-pressure maintaining function according to claim 1, characterized in that, The shaking table assembly further comprises: The first piston block is fixedly arranged at one end of the first resistance rod located in the second groove. The piston cavity is arranged in the inner part of the shaking plate. The inner wall of the piston cavity is sleeved with the second piston block. The inner wall of the piston cavity is fixedly arranged with a conduit. The other end of the conduit extends into the second groove. The side walls of the first piston block and the second piston block are in dynamic sealing design with the inner walls of the second groove and the piston cavity, respectively. One end of the second piston block away from the conduit is fixedly arranged with a guide rod extending into the shaking table to limit the movement of the second piston block. The limiting block is fixedly arranged on the inner wall top and the inner wall bottom of the second groove to limit the position of the first resistance rod. 3.The intelligent three-gas culture device with micro-pressure maintaining function of claim 2, wherein, The shaking table mechanism further comprises: The first servo motor is fixedly arranged on the inner wall bottom of the three-gas incubator. The inner wall top of the three-gas incubator is fixedly arranged with a second servo motor. The output shafts of the first servo motor and the second servo motor are respectively provided with a first transmission mechanism and a second transmission mechanism, so that the first servo motor and the second servo motor drive the threaded rod and the rotating sleeve to rotate through the first transmission mechanism and the second transmission mechanism, respectively. 4.The intelligent three-gas culture device with micro-pressure maintaining function of claim 1, wherein, The gas guide mechanism comprises: The first flow guide grid is fixedly arranged on the top of the second sealing plate. The top of the second sealing plate is fixedly arranged with a second flow guide grid. The first flow guide grid and the second flow guide grid are respectively located on the two sides of the mounting plate. The top of the first sealing plate is fixedly arranged with a first flow guide box and a second flow guide box on the two sides, respectively. The first flow guide box and the second flow guide box are connected with the first flow guide grid and the second flow guide grid, respectively. The pump body is fixedly arranged on the inner wall bottom of the three-gas incubator. The gas inlet end of the pump body is fixedly arranged with a first gas guide pipe. The first gas guide pipe is connected with the first flow guide box. The gas outlet end of the pump body is fixedly arranged with a second gas guide pipe.

5. The intelligent three-gas culture device with micro-pressure maintenance function according to claim 4, characterized in that, The gas guide mechanism further comprises: The gas mixing device is arranged on the inner wall bottom of the three-gas incubator. The gas inlet end of the gas mixing device is connected with the gas outlet end of the second gas guide pipe. The gas outlet end of the gas mixing device is fixedly arranged with a third gas guide pipe. The third gas guide pipe is connected with the second flow guide box. The fourth gas guide pipe is fixedly arranged on the outer wall of the third gas guide pipe and extends to the top of the second sealing plate. The outer wall of the third gas guide pipe is fixedly arranged with a fifth gas guide pipe. The outer walls of one end of the third gas guide pipe close to the second gas guide box, the fourth gas guide pipe, and the fifth gas guide pipe are all provided with valve bodies. 6.The intelligent three-gas culture device with micro-pressure maintaining function according to claim 5, wherein, The negative pressure assembly further comprises: The seventh air duct is fixedly arranged in the interior of the tray and connected with the sixth air duct, and the air inlet end of the seventh air duct extends to the outside of the tray to be connected with the fourth air duct. 7.The intelligent three-gas culture device with micro-pressure maintaining function of claim 1, wherein, The auxiliary assembly further comprises: An annular groove is arranged on the sidewall of the first connecting block; An arc-shaped plate is movably arranged on the top of the second connecting block and symmetrically arranged with the center point of the second connecting block; A moving piece is sleeved on the inner upper end of the second connecting block and extends to the top of the second connecting block, the top of the moving piece is fixedly connected with the bottom of the arc-shaped plate, a spring for elastically supporting the moving piece is sleeved in the second connecting block, and a moving groove for the movement of the moving piece and the expansion and contraction of the spring is arranged on the top of the second connecting block.

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