Pressing device, detection device, fluid driving method and detection method

Abstract

The invention discloses a pressing device, a detection device, a fluid driving method and a detection method.The pressing device is used for pressing a closed chip consumable, the consumable comprises one or more mixing cavities which can deform under the action of external force, and mixed fluid channels connected with inlets and / or outlets of the one or more mixing cavities correspondingly; the pressing equipment comprises a pressing mechanism used for pressing the mixing cavity and a path sealing mechanism used for sealing the mixed fluid channel, the pressing mechanism comprises a pressing assembly capable of doing reciprocating motion in the pressing direction, and the pressing assembly comprises a pressing head abutting against the mixing cavity; the road sealing assembly comprises an abutting head used for abutting against the mixed fluid channel. The pressing equipment is not in contact with fluid in the fluid mixing and transferring processes, so that the risk of cross contamination is avoided; the mixed fluid channel is blocked when the fluid is mixed, so that the fluid mixing effect can be prevented from being influenced by fluid leakage.

Description

Technical Field

[0001] This invention relates to the field of medical testing technology, specifically to a pressing device, a testing device, a fluid driving method, and a testing method. Background Technology

[0002] In clinical medicine, blood cell testing and immune testing have important therapeutic significance. Through blood cell testing and immune testing, we can intuitively understand the occurrence and development of diseases. There are already many blood cell testing and immune testing devices in existing medical equipment, and even some devices that can perform both blood cell testing and immune testing have been launched one after another. The research and development and production of these devices have greatly improved medical efficiency.

[0003] Existing blood cell and immunoassay testing equipment mostly uses open-type consumables, and the equipment itself is designed accordingly. These devices include pipette tips, which are used to transfer and mix the analyte during testing. The analyte is directly exposed to the air, which can lead to dripping and contamination, potentially contaminating the equipment and causing inaccuracies in subsequent samples, thus reducing testing precision. Furthermore, using pipette tips to transfer fluids only allows for the mixing of one type of fluid at a time, resulting in low efficiency. Summary of the Invention

[0004] The purpose of this invention is to address the shortcomings of existing technologies by providing a pressing device for closed consumables.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] A pressing device for pressing a sealed chip consumable, the consumable including one or more deformable mixing chambers under external force, and mixing fluid channels respectively connected to the inlet and / or outlet of one or more of the mixing chambers, the pressing device comprising:

[0007] A pressing mechanism for pressing the mixing chamber, the pressing mechanism including a pressing assembly capable of reciprocating along the pressing direction, the pressing assembly including a pressing head that presses against the mixing chamber;

[0008] A blocking mechanism for blocking the mixed fluid passage, the blocking mechanism including a blocking assembly including a pressing head for pressing against the mixed fluid passage.

[0009] In some embodiments, the number of pressing heads is less than the number of mixing chambers; preferably, the number of mixing chambers is an integer multiple of the number of pressing heads included in the pressing assembly.

[0010] In some embodiments, there are 1, 2, 3, 4, 5, 6 or more pressing heads. When there are multiple pressing heads, the multiple pressing heads are distributed in parallel at intervals.

[0011] In some embodiments, the pressing assembly further includes an elastic element for driving the pressing head in a direction that tends to move away from the mixing chamber;

[0012] And / or, the pressing assembly further includes a pressing rod, the elastic element is integrated into the pressing rod to enable the pressing rod to extend and retract in the pressing direction, and the pressing head is disposed on the pressing rod;

[0013] And / or, each of the pressing components further includes a connecting seat, on which the pressing rod is disposed.

[0014] In some embodiments, the pressing mechanism further includes a driving device for driving the pressing assembly to move. The driving device includes a motor and a transmission mechanism. The pressing assembly is connected to the motor via the transmission mechanism, which is a lead screw and nut mechanism, a cam mechanism, or a gear and rack mechanism; and / or,

[0015] The sealing mechanism and the pressing mechanism are arranged adjacent to each other, and a separable connection structure is provided between them. The connection structure has a connected state and a separated state. When the connection structure is in the connected state, the pressing mechanism drives the sealing assembly to move away from the mixing chamber.

[0016] In some embodiments, each of the mixing chambers includes a first mixing chamber and a second mixing chamber that are interconnected. The pressing assembly includes a first pressing assembly for pressing the first mixing chamber and a second pressing assembly for pressing the second mixing chamber. The pressing mechanism further includes a driving device for driving the first pressing assembly and the second pressing assembly to move. The first pressing assembly and the second pressing assembly are driven independently by the driving device, or the first pressing assembly and the second pressing assembly are driven simultaneously by the driving device and move in the same or opposite directions.

[0017] In some embodiments, the driving device includes a first motor, a first lead screw and nut mechanism, a second motor, and a second lead screw and nut mechanism. The first pressing component is connected to the first motor through the first lead screw and nut mechanism, and the second pressing component is connected to the second motor through the second lead screw and nut mechanism.

[0018] In some embodiments, the driving device includes a motor, one or more rotatably disposed cams, the cams being disposed corresponding to the mixing chamber, the one or more cams being disposed on the same camshaft, the motor being connected to the camshaft, the first pressing component and the second pressing component respectively abutting against the outer contour surface of the cam, and the driving device further includes an elastic element connected to the pressing component for driving the pressing component to move in a direction tending away from the mixing chamber.

[0019] In some embodiments, the outer contour surface of the cam includes a first region, the first region including a central abutment located at the center, a first abutment and a second abutment respectively disposed on opposite sides of the central abutment, and the distance from each point on the first abutment and the second abutment to the camshaft gradually increases along the direction away from the central abutment;

[0020] The outer contour surface of the cam also includes a second region sequentially connected to the first region, and within the second region, all points on the outer contour surface of the cam are at the same distance from the camshaft.

[0021] In some embodiments, a paddle is fixedly disposed on the camshaft. When the cam rotates to a set angle, the paddle engages with the sealing assembly to drive the sealing assembly to move away from the mixing fluid channel when the cam rotates.

[0022] In some embodiments, the number of pressure heads is less than the number of mixing fluid channels; preferably, the number of mixing fluid channels is an integer multiple of the number of pressure heads; and / or, the number of pressure heads is 1, 2, 3, 4, 5, 6 or more, and when there are multiple pressure heads, the multiple pressure heads are distributed in parallel and spaced apart; and / or, the number of pressure heads is the same as the number of pressing heads.

[0023] In some embodiments, the road sealing assembly further includes a pressure seat, the pressure head is disposed on the pressure seat, and the road sealing assembly further includes a heating element disposed on the pressure seat for heating the pressure head and a temperature sensor disposed on the pressure seat for monitoring the heating temperature of the heating element.

[0024] In some embodiments, the sealing mechanism further includes an elastic element for driving the sealing assembly in a direction tending toward the mixing fluid channel, the sealing assembly having a highest position and a lowest position, wherein when the sealing assembly is in the lowest position, the pressure head presses against the mixing fluid channel; and when the sealing assembly is in the highest position, the pressure head moves away from the mixing fluid channel and the elastic element is in a compressed state.

[0025] In some embodiments, the road-blocking mechanism further includes a locking mechanism for locking the road-blocking assembly at its highest position. The locking mechanism includes a slidably disposed locking member. When the locking mechanism is in a locked state, the locking member is located on the movement path of the road-blocking assembly. When the locking mechanism is in an unlocked state, the locking member leaves the movement path of the road-blocking assembly.

[0026] In some embodiments, the lower end of the locking member is provided with an inclined surface that is inclined toward the direction of the mixing chamber and away from the movement path of the sealing assembly. When the sealing assembly moves away from the mixing fluid channel, the sealing assembly can engage with the inclined surface to drive the locking member to slide away from the movement path of the sealing assembly.

[0027] In some embodiments, the locking element is an electromagnet. When the electromagnet is de-energized, the locking element blocks the movement path of the road-blocking assembly. When the electromagnet is energized, the locking element moves away from the movement path of the road-blocking assembly.

[0028] In some embodiments, one of the pressing component and the road-blocking component is provided with a mating part, and the other is provided with a limiting member. The pressing component has an initial position, and when the pressing component is in the initial position, the road-blocking component is in the highest position. The limiting member is engaged with the mating part.

[0029] In some embodiments, the pressing component has a highest position and a lowest position when it reciprocates to press the mixing chamber, and the initial position is located above the highest position of the pressing component during reciprocating motion.

[0030] When the limiting member is disposed on the pressing seat and the mating part is disposed on the connecting seat, when the pressing component reciprocates to the highest position, the mating part is located below the limiting member;

[0031] When the limiting member is disposed on the connecting seat and the mating part is disposed on the pressing seat, when the pressing component reciprocates to the highest position, the limiting member is located below the mating part.

[0032] In some embodiments, the sealing mechanism further includes a motor and a transmission mechanism. The sealing assembly is connected to the motor via the transmission mechanism to drive the sealing assembly to move toward the mixing fluid channel. The transmission mechanism is a lead screw and nut mechanism or a gear and rack mechanism.

[0033] In some embodiments, the consumable is further provided with a premixing chamber that is deformable under external force and a premixed fluid channel connected to the inlet and / or outlet of the premixing chamber. The pressing device further includes a blocking mechanism, which includes a blocking component for pressing against the premixed fluid channel.

[0034] In some embodiments, the blocking mechanism further includes an elastic member for driving the blocking assembly in a direction tending toward the premixed fluid channel, the blocking assembly having a highest position and a lowest position, wherein when the blocking assembly is in the lowest position, the blocking assembly presses against the premixed fluid channel; and when the blocking assembly is in the highest position, the blocking assembly moves away from the premixed fluid channel and the elastic member is in a compressed state.

[0035] The blocking mechanism further includes a locking mechanism for locking the blocking component at its highest position. The locking mechanism includes a slidably disposed locking member. When the locking mechanism is in a locked state, the locking member is located on the movement path of the blocking component. When the locking mechanism is in an unlocked state, the locking member leaves the movement path of the blocking component.

[0036] The blocking mechanism is located adjacent to the pressing mechanism, and a separable connecting structure is provided between them. The connecting structure has a connected state and a separated state. When the connecting structure is in the connected state, the pressing mechanism drives the blocking component to move away from the mixing chamber.

[0037] The present invention also provides a detection device having a pressing device as described in any of the preceding claims and a pusher mechanism for pushing a sample into the mixing chamber.

[0038] In some embodiments, the consumable includes a main body, the mixing chamber and the mixing fluid channel are both disposed on the main body, the main body is further provided with a sample inlet, a dispensing chamber, one or more metering chambers, and an overflow chamber, the sample inlet, one or more metering chambers and the overflow chamber are respectively connected to the dispensing chamber, one or more metering chambers are connected to one or more mixing chambers in a one-to-one correspondence, and plugs are slidably disposed in one or more metering chambers and the overflow chamber, the consumable also includes a delivery tube assembly disposed at the sample inlet, and the ejector mechanism includes:

[0039] Ejector seat;

[0040] A first ejector assembly is used to push the sample in the delivery tube group into the body. The first ejector assembly includes a first ejector pin disposed on the ejector pin seat and an elastic member disposed between the ejector pin seat and the first ejector pin.

[0041] The second ejector assembly is used to push the plug in the overflow cavity to block the inlet of the overflow cavity. The second ejector assembly includes a second ejector pin disposed on the ejector pin seat and an elastic member disposed between the ejector pin seat and the second ejector pin.

[0042] One or more third ejector pin assemblies are used to push the plug in the metering chamber to push fluid into the mixing chamber. The third ejector pin assembly includes a third ejector pin disposed on the ejector pin seat and an elastic member disposed between the ejector pin seat and the third ejector pin. The third ejector pin assembly is configured in a one-to-one correspondence with the metering chamber.

[0043] In some embodiments, the second ejector pin and the third ejector pin are disposed on the same side of the ejector pin seat, and the length of the second ejector pin is greater than the length of the third ejector pin;

[0044] When the third ejector assembly corresponds to the position of the metering cavity, the second ejector assembly is offset from the position of the overflow cavity.

[0045] In some embodiments, the detection device further includes a transfer mechanism for driving the consumable to move and adjust its position. The transfer mechanism includes an X-axis drive platform for driving the consumable to move along the X-axis direction and a Y-axis drive platform for driving the consumable to move along the Y-axis direction. The X-axis drive platform is disposed on the Y-axis drive platform, and the consumable is disposed on the X-axis drive platform.

[0046] In some embodiments, the detection device further includes a heating monitoring module for monitoring the temperature of a sample in the consumable. The heating monitoring module includes a heating element and a temperature sensor, both of which are disposed on the X-axis drive platform and located at the bottom of the consumable.

[0047] In some embodiments, the detection device further includes a detection mechanism, which includes a microscopic imaging component for photographing fluid samples. The microscopic imaging component is movably disposed along directions toward and away from the consumable. The detection mechanism also includes a drive mechanism for driving the microscopic imaging component to move.

[0048] And / or, the detection mechanism further includes a fixedly installed camera and a supplementary light;

[0049] And / or, the bottom of the consumable is provided with a detection cavity for absorbance detection, and the detection mechanism further includes a light source module for absorbance detection. The detection module includes a light source mounting base, an LED light source mounted on the light source mounting base, a receiver mounting base, and a receiver mounted on the receiver mounting base. The light source mounting base and the receiver mounting base are both mounted on the X-axis drive platform and located on opposite sides of the detection cavity.

[0050] In some embodiments, the drive mechanism includes a motor and a reducer connected to the motor. The reducer is a synchronous belt reducer, which includes a housing and a synchronous belt disposed within the housing. The microscopic imaging assembly is connected to the synchronous belt. The drive mechanism also includes a constant force spring connected between the housing and the microscopic imaging assembly.

[0051] The present invention also provides a fluid-driven method, wherein the contents in the mixing chamber are mixed and flow out of the mixing chamber by a pressing device as described in any one of the preceding claims, the fluid-driven method comprising:

[0052] The sealing mechanism is used to seal the mixing fluid channel, and then the pressing mechanism is used to reciprocate to press the mixing chamber to mix its contents.

[0053] After the mixing is completed, the sealing mechanism is released from the mixing fluid channel, and the pressing mechanism is used to press the mixing chamber to allow the fluid inside to flow out of the mixing chamber.

[0054] In some embodiments, the mixing fluid channel is provided with a sealing structure for blocking the mixing fluid channel. The sealing structure can be destroyed by the pressure when the contents of the mixing chamber flow out of the mixing chamber. When the sealing mechanism is used to seal the mixing fluid channel, the pressure head is pressed against the mixing fluid channel and the pressure position is located on the side of the sealing structure away from the mixing chamber. Preferably, there is a gap between the pressure position and the sealing structure; the gap is preferably 0.5 to 1 mm.

[0055] In some embodiments, when the pressing assembly presses the mixing chamber to cause its contents to flow out of the mixing chamber, the pressing head presses against the mixing fluid channel when the sealing structure is intact; when the sealing structure is damaged, the pressing head leaves the mixing fluid channel.

[0056] The present invention also provides a detection method, wherein detection is performed using the detection device described in any one of the above claims, the detection method comprising:

[0057] S1, push the sample into the consumable;

[0058] S2, after the contents in the mixing chamber are mixed evenly by any of the above-described fluid driving methods, the fluid in the mixing chamber flows out of the mixing chamber;

[0059] S3, proceed with the testing.

[0060] Due to the application of the above technical solution, the pressing device of the present invention has the following advantages compared with the prior art: The pressing device uses the reciprocating motion of the pressing component to press and mix the contents of the mixing chamber. After the contents in the mixing chamber are mixed, the pressing component presses the mixing chamber to cause the fluid to flow out of the mixing chamber. During the fluid mixing and transfer process, there is always no contact between the device and the fluid, greatly avoiding the risk of cross-contamination. Furthermore, during the fluid mixing process, the mixing fluid channel can be sealed by a sealing mechanism to prevent leakage that could affect the mixing effect, thus ensuring the accuracy and stability of the detection. Attached Figure Description

[0061] Appendix Figure 1 This is a three-dimensional schematic diagram of the closed-type chip consumable in Embodiment 1.

[0062] Appendix Figure 2 This is an exploded view of the enclosed chip consumable in Embodiment 1.

[0063] Appendix Figure 3 This is a three-dimensional schematic diagram of the base of the enclosed chip consumable in Embodiment 1 after removing part of its structure.

[0064] Appendix Figure 4 This is a top view of the base of the enclosed chip consumable in this embodiment after part of the structure has been removed;

[0065] Appendix Figure 5 For the appendix Figure 4 sectional view along line AA;

[0066] Appendix Figure 6 This is a top view of the base of the enclosed chip consumable in this embodiment after part of the structure has been removed;

[0067] Appendix Figure 7 For the appendix Figure 6 Enlarged view of a portion of point A in the middle;

[0068] Appendix Figure 8 This is a top view of the base of the enclosed chip consumable in this embodiment after part of the structure has been removed;

[0069] Appendix Figure 9 For the appendix Figure 8 sectional view along line AA;

[0070] Appendix Figure 10 For the appendix Figure 8 sectional view along line BB;

[0071] Appendix Figure 11 This is a bottom view of the base in the enclosed chip consumable in this embodiment;

[0072] Appendix Figure 12 This is a schematic diagram of the structure of the closed-loop chip consumable in this embodiment, excluding the sample inlet.

[0073] Appendix Figure 13 For the appendix Figure 12 sectional view along line AA;

[0074] Appendix Figure 14 For the appendix Figure 13 Enlarged view of a portion of point A in the middle;

[0075] Appendix Figure 15 This is a three-dimensional schematic diagram of the dilution tube assembly in the closed-type chip consumable of this embodiment 2;

[0076] Appendix Figure 16 This is a top view of the dilution tube assembly in the closed-type chip consumable of this embodiment 2;

[0077] Appendix Figure 17 For the appendix Figure 16 sectional view along line AA;

[0078] Appendix Figure 18 This is one of the three-dimensional schematic diagrams of the pressing device in Embodiment 3;

[0079] Appendix Figure 19 This is the second three-dimensional schematic diagram of the pressing device in Embodiment 3;

[0080] Appendix Figure 20 This is the third perspective view of the pressing device in this embodiment 3;

[0081] Appendix Figure 21 This is the fourth perspective view of the pressing device in Embodiment 3;

[0082] Appendix Figure 22 This is one of the three-dimensional schematic diagrams of the pressing device in Embodiment 4;

[0083] Appendix Figure 23 This is the second three-dimensional schematic diagram of the pressing device in Embodiment 4;

[0084] Appendix Figure 24 This is a three-dimensional schematic diagram of the pressing device in Embodiment 4 after removing part of its structure;

[0085] Appendix Figure 25 This is a three-dimensional schematic diagram of the cam in the pressing device of Embodiment 4;

[0086] Appendix Figure 26This is a three-dimensional schematic diagram of the pressing component in the pressing device of Embodiment 4;

[0087] Appendix Figure 27 This is a three-dimensional schematic diagram of the detection equipment in this embodiment;

[0088] Appendix Figure 28 This is a three-dimensional schematic diagram of the ejector pin mechanism in the testing device of this embodiment;

[0089] Appendix Figure 29 This is a top view of the ejector pin mechanism in the testing device of this embodiment;

[0090] Appendix Figure 30 For the appendix Figure 29 sectional view along line AA;

[0091] Appendix Figure 31 For the appendix Figure 29 sectional view along line BB;

[0092] Appendix Figure 32 This is a three-dimensional schematic diagram of the imaging device in the detection equipment of this embodiment;

[0093] Appendix Figure 33 This is a three-dimensional schematic diagram of the imaging device in the detection equipment of this embodiment after removing part of its structure;

[0094] Appendix Figure 34 This is an exploded view of the light source module in the detection device of this embodiment;

[0095] Appendix Figure 35 This is a schematic diagram of the structure on the mounting tray of the heating and constant temperature monitoring module in the testing equipment of this embodiment.

[0096] The components are as follows: 1. Main body; 1a. Base plate; 1b. Lower membrane; 1c. Upper membrane; 1c1. Cover membrane; 1d. Detection chamber cover plate; 1e. Sealing structure; 10. Sample inlet; 111. Quantitative chamber; 112. Sample outlet channel; 113. Exhaust port; 114. Distribution chamber; 115. Overflow chamber; 116. Plug; 121. First mixing chamber; 122. Second mixing chamber; 123. Narrow channel; 124. Mixed fluid channel; 131. First detection chamber; 132. Waste liquid channel; 133. Waste liquid chamber; 134. Buffer chamber; 141. Temporary storage chamber; 142. Second detection chamber; 143. Waste liquid chamber; 15. Third detection chamber; 161. First premixing chamber; 162. Second premixing chamber; 163. Narrow channel; 164. Premixed fluid channel; 17. Fourth detection chamber; 81. Reagent channel; 182. First reagent storage chamber; 183. Second reagent storage chamber; 183. Narrow channel; 19. Sealing plug; 191. Sealing layer; 192. Sealing part;

[0097] 21. Delivery tube assembly; 211. Body; 212. Sampling tube; 213. Sealing piston; 22. Dilution tube assembly; 221. Sampling tube; 2211. Tube body; 2212. Sampling section; 2213. Central channel; 2214. Opening; 222. Dilution tube; 223. Sealing piston;

[0098] 301. Support plate; 302. Bracket; 311. Pressing head; 312. Pressing rod; 313. Connecting seat; 314. Mating part; 321. First motor; 322. First lead screw and nut mechanism; 323. Second motor; 324. Second lead screw and nut mechanism; 331. Pressing head; 332. Pressing seat; 3321. Limiting part; 333. Abutting rod; 334. Blocking part; 335. Heating element; 336. Temperature sensor; 34. Elastic element; 35. Locking element; 351. Inclined surface; 361. Blocking head; 362. Blocking rod; 363. Blocking part; 37. Elastic element; 38. Locking mechanism;

[0099] 41. Motor; 42. Cam; 421. First region; 4211. First abutment; 4212. Second abutment; 4213. Second abutment; 422. Second region; 43. Camshaft; 44. Elastic element; 451. Motor; 452. Transmission mechanism; 453. Connecting plate; 46. Elastic element; 471. Paddle; 472. Pressure plate;

[0100] 5. Base;

[0101] 61. X-axis moving platform; 611. Pallet; 62. Y-axis moving platform;

[0102] 7. Ejector mechanism; 71. Ejector seat; 72. First ejector pin; 73. Elastic element; 74. Second ejector pin; 73. Third ejector pin;

[0103] 81. Microscopic imaging assembly; 811. Lens; 821. Motor; 822. Reducer; 823. Rack; 824. Gear; 83. Mount; 841. Camera; 842. Fill light; 851. Light source mount; 852. LED light source; 853. Receiver mount; 854. Receiver;

[0104] 91. Heating element; 92. Sensor. Detailed Implementation

[0105] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0106] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. This is only for the convenience of describing the invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0107] Example 1

[0108] like Figure 1 and Figure 2 As shown, the enclosed chip consumable in this embodiment includes a main body 1 and a delivery tube assembly 21.

[0109] The main body 1 is provided with a sample inlet 10, a quantitative zone, a mixing zone and a detection unit. The quantitative zone, the mixing zone and the detection unit are connected in sequence. The sample enters the main body through the sample inlet 10, passes through the quantitative zone and the mixing zone in sequence and then goes to the detection unit for detection.

[0110] The detection unit includes a first detection unit, which can be used for blood cell detection. One or more quantitative zones, mixing zones, and the first detection unit are provided, and each of these zones corresponds to one another.

[0111] like Figures 1-3 As shown, each quantitative zone includes a quantitative cavity 111 connected to the sample inlet 10 and a sample outlet channel 112 connected to the quantitative cavity 111. The sample outlet channel 112 is connected to the mixing zone.

[0112] The metering chamber 111 is configured to guide a set volume of fluid sample to the mixing zone. Specifically, the volume of the metering chamber 111 is fixed. After the fluid enters and fills the metering chamber 111, the volume of the fluid in the metering chamber 111 is fixed. All the fluid in the metering chamber 111 enters the mixing zone for mixing to ensure the volume of fluid entering the mixing zone.

[0113] The metering chamber 111 and the sample outlet channel 112 are connected via an exhaust port 113 formed on the main body 1. A waterproof and breathable membrane is provided at the exhaust port 113. When fluid flows into the metering chamber 111, the gas in the metering chamber 111 can be discharged from the exhaust port 113, thus ensuring that the metering chamber 111 is filled with fluid. The waterproof and breathable membrane provided at the exhaust port 113 is preferably resistant to hydrostatic pressure of 200 kPa or higher and has a permeability of 800 ml / min / cm. 2 A composite PTFE membrane with a pressure resistance of over / kPa is used to prevent fluids with insufficient pressure resistance from leaking directly from the waterproof and breathable membrane.

[0114] like Figure 2 and Figure 3 As shown, the main body 1 is also provided with a distribution cavity 114. The distribution cavity 114 is located between the sample inlet 10 and each quantitative cavity 111. The fluid entering the main body 1 is distributed by the distribution cavity 114 and then enters each quantitative cavity 111, thereby ensuring that the fluid fills each quantitative cavity 111.

[0115] The dispensing cavity 114 is a long and narrow channel, and the cross-sectional area of ​​each metering cavity 111 is larger than the cross-sectional area of ​​the dispensing cavity 114. For example... Figure 2 and Figure 3 As shown, the main body also includes an overflow chamber 115 connected to the distribution chamber 114. The distance between the inlet of each metering chamber 111 and the inlet of the distribution chamber 114 is smaller than the distance between the inlet of the overflow chamber 115 and the inlet of the distribution chamber 114. Since the cross-sectional area of ​​the metering chamber 111 is relatively large, the fluid resistance is very small, while the cross-sectional area of ​​the distribution chamber 114 is relatively small, resulting in greater fluid resistance. Therefore, when fluid flows in the distribution chamber 114, if it reaches the inlet of the metering chamber 111, it will preferentially fill the metering chamber 111. According to this principle, after the fluid fills all the metering chambers 111, the excess fluid will flow into the overflow chamber 115. Therefore, when introducing fluid into the distribution chamber 114, a slightly excessive amount of fluid is injected so that the overflow chamber 115 is also filled with a certain volume of fluid, ensuring that the fluid fills each metering chamber 111.

[0116] When multiple quantitative zones are provided, it is preferable that each quantitative cavity 111 and overflow cavity 115 are arranged in parallel, and the extension direction of the distribution cavity 122 is perpendicular to the extension direction of each quantitative cavity 111 and overflow cavity 115. This arrangement can reduce the structural volume of the main body 1.

[0117] Both the metering chamber 111 and the overflow chamber 115 extend to the outside of the main body 1, such as Figure 1 and Figure 2 As shown, the consumable also includes a plug 116, which seals one end of each metering chamber 111 and overflow chamber 115 extending to the outside of the main body. The plug 116 is slidably disposed along the extending direction of each metering chamber 111 and overflow chamber 115. While sealing each metering chamber 111, the plug 116 can also be pushed to slide within the metering chamber 111, squeezing and pushing the fluid in the metering chamber 111, thereby pushing all the fluid in the metering chamber 111 into the mixing zone. The purpose of setting the plug 116 in the overflow chamber 115 is to block the inlet of the overflow chamber 115 when the plug 116 in the metering chamber 111 is pushed to transfer fluid, thereby preventing the fluid in the metering chamber 111 from entering the overflow chamber 115. However, this does not guarantee that all the fluid in the metering chamber 111 is pushed into the mixing zone, and thus cannot achieve quantitative fluid delivery.

[0118] like Figure 2 As shown, the mixing zone includes a mixing chamber and a mixing fluid channel 124 connected to the outlet of the mixing chamber, and a sample outlet channel 112 connected to the inlet of the mixing chamber.

[0119] The mixing fluid channel 124 has a blocked state and an unblocked state. When it is in the blocked state, the mixing chamber is isolated from the first detection unit. When it is in the unblocked state, the mixing chamber and the first detection unit are connected through the mixing fluid channel 124.

[0120] The sample outlet channel 112 has a blocked state and an unblocked state. When it is in the blocked state, the metering chamber 111 and the mixing chamber are isolated. When it is in the unblocked state, the metering chamber 111 and the mixing chamber are connected through the sample outlet channel 112.

[0121] The mixing chamber contains reagents. Before testing, both the mixing fluid channel 124 and the sample outlet channel 112 are sealed, thus encapsulating the reagents within the mixing chamber.

[0122] The mixing chamber can be compressed, and after compression, the contents within it flow and are mixed to form a mixed fluid.

[0123] The mixing chamber has at least one compartment. In this embodiment, such as... Figures 1-6 and Figure 8 As shown, the mixing chamber includes a first mixing chamber 121 and a second mixing chamber 122. The first mixing chamber 121 and the second mixing chamber 122 are connected by a narrow channel 123. The sample outlet channel 112 is connected to the first mixing chamber 121, and the mixing fluid channel 124 is connected to the second mixing chamber 122.

[0124] The narrow channel 123 allows the fluid to increase its velocity at the narrow channel 123 when it flows back and forth between the first mixing chamber 121 and the second mixing chamber 122, thereby increasing the mixing effect.

[0125] Preferably, the depth of the first mixing chamber 121 and the second mixing chamber 122 is no greater than 5 mm, as a larger depth is not conducive to fluid mixing. The bottom and corners of the first mixing chamber 121 and the second mixing chamber 122 are rounded to reduce fluid sample residue in dead corners.

[0126] like Figure 2 and Figure 11 As shown, the first detection unit includes a first detection chamber 131, a waste liquid channel 132, and a waste liquid chamber 133 connected in sequence.

[0127] The first detection cavity 131 is a flat cavity. Specifically, the height of the first detection cavity is 0.1–0.4 mm, and the cross-sectional area of ​​the first detection cavity is 50–70 mm². 2The volume of the first detection chamber is approximately 10–25 μL. The first detection chamber 131 has an inlet for fluid to flow in and an outlet for fluid to flow out. Both the inlet and the outlet are located at the bottom of the first detection chamber 131, and the distance between the inlet and the outlet is the greatest at the bottom of the first detection chamber 131.

[0128] The first detection chamber 131, the waste liquid channel 132, and the waste liquid chamber 133 are sequentially connected. When fluid enters the first detection chamber 131, it quickly fills the first detection chamber 131. When the fluid in the first detection chamber 131 is not driven or is driven but the driving force is less than a set value, the fluid will not automatically exit from the first detection chamber 131. When the fluid in the first detection chamber 131 is driven or is driven with a driving force greater than or equal to the set value, the fluid exits from the first detection chamber 131 and enters the waste liquid chamber 133 through the waste liquid channel 132.

[0129] like Figures 2-4 , Figure 6 and Figure 8 As shown, the first detection unit further includes a buffer chamber 134 disposed between the mixing fluid channel 124 and the first detection chamber 131. The buffer chamber 134 is connected to both the mixing fluid channel 124 and the first detection chamber 131, and the flow direction of the fluid in the buffer chamber 134 is perpendicular to the flow direction of the fluid in the mixing fluid channel 124. This arrangement can slow down the flow rate of the fluid entering the first detection chamber 131, while allowing some air bubbles in the fluid to remain in the buffer chamber 134 instead of flowing into the first detection chamber 131, thereby improving the detection effect.

[0130] The detection unit may also include a second detection unit, which can be used for immune detection, such as for the detection of SAA, CRP, etc.

[0131] like Figure 8 and Figure 9 As shown, the second detection unit includes a temporary storage chamber 141, a second detection chamber 142, and a waste liquid chamber 143. A test strip is disposed in the second detection chamber 142. The temporary storage chamber 141 is connected to the second detection chamber 142 and the waste liquid chamber 143 respectively. The quantitative chamber 111, the sample outlet channel 112, the mixing chamber, the mixing fluid channel 124, and the temporary storage chamber 141 are connected in a corresponding manner.

[0132] The temporary storage chamber 141 is a cylindrical cavity with a fixed volume. There is a small hole at the bottom that connects to the second detection chamber 142. After the fluid fills the temporary storage chamber 141, the fluid flows directly through the small hole at the bottom of the temporary storage chamber 141 to the top of the test strip and is absorbed.

[0133] The waste liquid chamber 143 of the second detection unit can be shared with the waste liquid chamber 133 of the first detection unit, or they can be set up independently.

[0134] The detection unit may also include a third detection unit, which can be used for hemoglobin detection.

[0135] like Figures 3-5 As shown, the third detection unit includes a third detection chamber 15 for absorbance detection, and a quantitative chamber 111, a sample outlet channel 112, a mixing chamber, a mixing fluid channel 124 and the third detection chamber 15 are connected in a one-to-one correspondence.

[0136] In this embodiment, the chip consumable also includes a pre-dilution region for pre-dilution of the sample, which is formed within the main body 1.

[0137] like Figure 2 As shown, the pre-dilution zone includes a premixing chamber disposed within the main body 1 and a premixed fluid channel 164 connected to the outlet of the premixing chamber. The premixing chamber is connected to the sample inlet 10, and the premixed fluid channel 164 is connected to the inlet of the dispensing chamber 114.

[0138] The premixed fluid channel 164 has a blocked state and an unblocked state. When it is in the blocked state, the distribution chamber 114 is isolated from the premixing chamber. When it is in the unblocked state, the distribution chamber 114 and the premixing chamber are connected through the premixed fluid channel 164.

[0139] The premixing chamber stores reagents. Before detection, the premixed fluid channel 164 is blocked and the sample inlet 10 is sealed, thus encapsulating the reagents within the premixing chamber.

[0140] The premixing chamber can be compressed, and after compression, the contents within the premixing chamber flow and are mixed to form a mixed fluid.

[0141] The premixing chamber has at least one compartment. In this embodiment, such as... Figures 1-4 , Figure 6 and Figure 8 As shown, the premixing chamber includes a first premixing chamber 161 and a second premixing chamber 162. The first premixing chamber 161 and the second premixing chamber 162 are connected by a narrow channel 163. The sample inlet 10 is connected to the first premixing chamber 161, and the premixed fluid channel 164 is connected to the second premixing chamber 162.

[0142] The narrow channel 163 allows the fluid to increase its velocity at the narrow channel 163 when it flows back and forth between the first premixing chamber 161 and the second premixing chamber 162, thereby increasing the mixing effect.

[0143] Preferably, the depth of the first premixing chamber 161 and the second premixing chamber 162 is no more than 5 mm, as a larger depth is not conducive to the mixing of fluids.

[0144] The detection unit also includes a fourth detection unit and a reagent storage area. The fourth detection unit can be used for immunoassays, such as PCT testing. Figures 2-4 , Figure 6 , Figure 8 and Figure 10 As shown, the fourth detection unit includes a fourth detection chamber 17 and a test strip disposed within the fourth detection chamber 17. During PCT testing, no sample dilution is required; therefore, the fourth detection chamber 17 is directly connected to the sample inlet 10.

[0145] The reagent storage area includes a reagent storage cavity and a reagent channel 181 connected to the outlet of the reagent storage cavity. The reagent channel 181 is connected to the fourth detection cavity 17.

[0146] The reagent storage chamber contains reagents. The reagent storage chamber can be pressed, and after being pressed, the reagents stored inside flow out of the reagent storage chamber.

[0147] The reagent channel 181 has a blocked state and an unblocked state. When it is in the blocked state, the reagent storage chamber and the fourth detection chamber are isolated. When it is in the unblocked state, the reagent storage chamber and the fourth detection chamber 17 are connected through the reagent channel 181.

[0148] For ease of operation, the reagent storage chamber has the same structural form as the mixing chamber and the premixing chamber. Specifically, the reagent storage chamber includes a first reagent storage chamber 182 and a second reagent storage chamber 183. The first reagent storage chamber 182 and the second reagent storage chamber 183 are connected by a narrow channel 184, and a reagent channel 181 is connected to the second reagent storage chamber 183. Figures 2-4 , Figure 6 and Figure 8 As shown.

[0149] When a fourth detection unit is provided, one or more sample inlets 10 are provided. In this embodiment, two sample inlets 10 are provided, one sample inlet 10 is connected to the premixing chamber, and the other sample inlet 10 is connected to the fourth detection chamber 17.

[0150] The delivery tube assembly 21 is used for sample collection, and it is connected to each sample inlet 10. For example... Figure 2As shown, the delivery tube assembly 21 includes a body 211 and a sampling tube 212 disposed at one end of the body 211. The sampling tube 212 is used to collect samples. A first channel is provided inside the sampling tube 212. After hydrophilic treatment, the sampling tube 212 can easily draw in samples through capillary action and fill the first channel to ensure volume. A second channel is provided inside the body 211, and the first channel and the second channel are connected. A sealing piston 213 is provided at the end of the second channel away from the first channel to block the first channel. The sealing piston 213 is slidably disposed in the second channel. By pushing the sealing piston 213 to slide in the second channel, the sample collected by the sampling tube 212 can be pushed from the sample inlet 10 into the premixing chamber and the fourth detection chamber 17.

[0151] like Figures 12-14 As shown, each sample inlet 10 is equipped with a sealing layer 191, which seals the sample inlet 10 when no test is being performed. When the delivery tube assembly 21 is connected to the sample inlet 10, the sampling tube 212 is inserted into the sample inlet 10, and the sampling tube 212 can pierce the sealing layer 191 and extend into the interior of the sample inlet 10.

[0152] The sealing layer 191 has a flat end face facing the conveying pipe assembly 21, and a thinning portion is provided on the other end face of the sealing layer 191 opposite to the one end face to reduce the thickness of the sealing layer 191.

[0153] Each sample inlet 10 is equipped with a sealing part 192. The sealing part 192 cooperates with the sampling tube 212 after the sampling tube 212 passes through the sealing layer 191 to prevent the sample from flowing out from the damaged part of the sealing layer 191.

[0154] In this embodiment, a sealing plug 19 is provided at each sample inlet 10. The sealing layer 191 and the sealing part 192 are integrally disposed on the sealing plug 19. The sealing part 192 is a through hole provided on the sealing plug 19, and the sampling tube 212 is interference-fitted with the through hole. This simplifies the processing technology and assembly.

[0155] In this embodiment, such as Figure 2 As shown, the main body includes a base plate 1a, a lower film 1b bonded to the base plate 1a, and an upper film 1c bonded to the lower film 1b. The base plate 1a is made of common transparent plastic materials, such as PMMA, COC, and PC. The lower film 1b is a thin film structure, and uses a material with certain thermal adhesion, preferably EVA, PET, PE, TPE, etc., and is bonded to the upper surface of the base plate 1a by adhesives or similar bonding agents. The upper film 1c also uses a material with certain thermoplastic properties, such as PE, PP, and PVC, and the lower film 1b and the upper film 1c are bonded together by ultrasonic welding, high-frequency welding, or high-temperature hot-melt welding.

[0156] The sample inlet 10, quantitative chamber 111, distribution chamber 114, overflow chamber 115, first detection chamber 131, waste liquid channel 132, waste liquid chamber 133, buffer chamber 134, temporary storage chamber 141, second detection chamber 142, waste liquid chamber 143, third detection chamber 15, and fourth detection chamber 17 are all located within the base plate 1a.

[0157] The base plate 1a covers the detection cover plate 1d at the positions corresponding to each of the first detection cavities 131. The detection cover plate 1d and the base plate 1a can be bonded by different methods, such as ultrasonic welding, laser welding, double-sided pressure-sensitive adhesive bonding, etc. The detection cavity cover plate 1d is preferably made of a transparent polymer with high light transmittance, such as PMMA, PC, etc.

[0158] The mixing chamber, premixing chamber, and reagent storage chamber each include a cavity set on the base plate 1a and a cover membrane set on the upper membrane 1c and located directly above the cavity. The lower membrane 1b has an opening corresponding to the position of the cavity. The cover membrane has an upwardly convex arc structure and is used for pressing to allow fluid to flow in the cavity. The upward convexity of the cover membrane is to allow the upper membrane 1c to deform in advance, preventing excessive deformation and breakage during the pressing process.

[0159] like Figure 7 As shown, the sample outlet channel 112, the mixing fluid channel 124, the premixed fluid channel 164, and the reagent channel 181 are all at least partially formed between the upper membrane 1c and the lower membrane 1b. Specifically, the upper membrane 1c and the lower membrane 1b are bonded together except at the locations of the sample outlet channel 112, the mixing fluid channel 124, the premixed fluid channel 164, and the reagent channel 181. The upper membrane 1c and the lower membrane 1b located at the locations of the sample outlet channel 112, the mixing fluid channel 124, the premixed fluid channel 164, and the reagent channel 181 are partially bonded together, and the bonded portions form sealing structures 1e, the length and width of which are approximately 0.5-2 mm.

[0160] The bond strength between the upper membrane 1c and the lower membrane 1b at sealing structure 1e is less than the bond strength between the upper membrane 1c and the lower membrane 1b at other locations besides sealing structure 1e. Therefore, sealing structure 1e can be destroyed by the fluid pressure in the channel. When sealing structure 1e is intact, the channel is in a blocked state. When sealing structure 1e is destroyed, the channel is in a non-blocked state.

[0161] By adjusting the welding parameters and area between the upper film 1c and the lower film 1b, areas with different bonding strengths can be created. For example, ultrasonic welding uses ultrasonic waves of different powers, while hot melt welding adjusts the temperature of the welding head and the contact time. Taking hot melt bonding of EVA film as an example, a welding temperature of 80-90℃ and a welding time of 5-10 seconds can be used at the sealing structure 1e. For other bonding areas between the upper film 1c and the lower film 1b, excluding the sealing structure 1e, a welding temperature of 100-120℃ and a welding time of 20-40 seconds can be used.

[0162] In this embodiment, the main body 1 has four sets of corresponding quantitative areas, mixing areas, and first detection units, which respectively detect red blood cell count, white blood cell count, five-part differential count, and platelet count. A set of corresponding quantitative areas, mixing areas, and second detection units is provided for simultaneous CRP and SAA immune detection. A set of corresponding quantitative areas, mixing areas, and third detection units is provided for hemoglobin detection. A set of fourth detection units is provided for PCT detection.

[0163] Example 2

[0164] In this embodiment, the pre-dilution zone is set independently of the main body 1, and the sample inlet 10 and the dispensing chamber 114 are connected through a channel set inside the main body 1. The consumables also include a dilution tube assembly 22 for acquiring and diluting the sample, the pre-dilution zone is formed in the dilution tube assembly 22, and the dilution tube assembly 22 is sealed and connected to the sample inlet 10.

[0165] like Figures 15-17 As shown, the dilution tube assembly 22 includes a sampling tube 221 for aspirating samples and a dilution tube 222 for storing diluent. One end of the sampling tube 221 is connected to the dilution tube 222, and the other end of the sampling tube 221 is positioned at the first inlet channel 101, and the connection between the two is sealed by a sealing ring.

[0166] The sampling tube 221 includes a tube body 2211 and a sampling section 2212 disposed at one end of the tube body 2211. The sampling tube 221 has a central channel 2213 inside, which passes through both ends of the tube body 2211 and the sampling section 2212 respectively. The sampling section 2212 is also provided with an opening 2214 that connects the central channel 2213 to the outside.

[0167] The dilution tube 222 has a cavity in which diluent can be stored. The volume of the diluent is set according to the test item. After the sampling unit 2212 absorbs the sample, it is placed in the cavity of the dilution tube 222.

[0168] After undergoing hydrophilic treatment, the sampling tube 221 can easily draw in the sample through capillary action and fill the central channel 2213 to ensure volume. The sample located in the central channel 2213 flows into the lumen of the dilution tube 222 through the opening 2214. The sample and diluent mix in the lumen of the dilution tube 222, thereby pre-diluting the sample.

[0169] like Figure 17 As shown, the dilution tube assembly 22 also includes a sealing piston 223, which seals the end of the dilution tube 222 away from the sampling tube 221. The sealing piston 223 is slidably disposed within the cavity of the dilution tube 222. By pushing the sealing piston 223 to slide within the cavity of the dilution tube 222, the fluid within the cavity of the dilution tube 222 can be pushed from the central channel 2213 of the sampling tube 221 into the main body 1.

[0170] Example 3

[0171] This embodiment also provides a pressing device for pressing the aforementioned enclosed chip consumable. In this embodiment, as... Figures 18-21 As shown, the pressing device includes a support plate 301, a pressing mechanism, and a sealing mechanism, both of which are mounted on the support plate 301.

[0172] The pressing mechanism is used to press the mixing chamber. The pressing mechanism includes a pressing component and a driving device. The pressing component is capable of reciprocating along the pressing direction, thereby pressing the mixing chamber. The driving device is used to drive the pressing component to move. In this embodiment, the pressing direction is vertical, with downward being the direction closer to the mixing chamber and upward being the direction farther from the mixing chamber; this direction is defined accordingly in the following description.

[0173] The pressing assembly includes a first pressing assembly and a second pressing assembly. Both the first pressing assembly and the second pressing assembly are located below the support plate 301. The first pressing assembly is used to press the first mixing chamber 121, and the second pressing assembly is used to press the second mixing chamber 122.

[0174] like Figures 19-21 As shown, both the first pressing assembly and the second pressing assembly include a pressing head 311, which is used to press against the first mixing chamber 121 and the second mixing chamber 122. The pressing head 311 is made of rubber material.

[0175] There may be one, two, three, four, five, six or more pressing heads 311. When there are multiple pressing heads 311, the multiple pressing heads 311 are distributed in parallel and at intervals.

[0176] The specific number of pressing heads 311 can be set according to the number of mixing chambers. Specifically, the number of pressing heads 311 in each pressing assembly is less than the number of mixing chambers; that is, the number of pressing heads 311 in the first pressing assembly is less than the number of the first mixing chambers 121, and the number of pressing heads 311 in the second pressing assembly is less than the number of the second mixing chambers 122. Preferably, the number of mixing chambers is an integer multiple of the number of pressing heads 311. In this embodiment, the number of first mixing chambers 121 is twice the number of pressing heads 311 in the first pressing assembly, and the number of second mixing chambers 122 is twice the number of pressing heads 311 in the second pressing assembly. This configuration improves detection efficiency while making the pressing device more compact and smaller in size.

[0177] The first pressing assembly and the second pressing assembly both include a pressing rod 312, which extends in the vertical direction. A pressing head 311 is disposed at the lower end of the pressing rod 312. The pressing rod 312 is provided with one, two, three, four, five, six or more pressing heads 311.

[0178] The first pressing assembly and the second pressing assembly may each include a connecting seat 313, and each pressing rod 312 of the first pressing assembly and the second pressing assembly is disposed on the corresponding connecting seat 313. In this way, the action of the connecting seat 313 can drive the pressing rods 312 to move synchronously, thereby realizing the simultaneous pressing of multiple mixing chambers.

[0179] Both the first and second pressing components may include elastic elements, which are directly or indirectly connected to the pressing head 311. The elastic force of the elastic element causes the pressing head 311 to move in a direction tending away from the mixing chamber. In this embodiment, the elastic element is integrated into the pressing rod 312, allowing the pressing rod 312 to extend and retract in the vertical direction. This allows the pressing head 311 to move synchronously when the pressing rod 312 extends and retracts. Even when multiple pressing heads 311 are provided and driven to move synchronously through the connecting seat 313, the pressing heads 311 can still be pressed to the bottom of the mixing chamber by extending and retracting the pressing rod 312, even if the multiple pressing heads 311 are not on the same horizontal plane. Moreover, the elastic element can buffer the movement of the pressing head, preventing damage to the consumables due to excessively fast movement speed.

[0180] In this embodiment, the pressing rod 312 includes a cylinder seat and a plunger slidably disposed within the cylinder seat. An elastic element is disposed between the cylinder seat and the plunger, forming a spring-plunger structure between the cylinder seat, the plunger, and the elastic element. The cylinder seat is connected to the connecting seat 313, and the pressing head 311 is connected to the plunger.

[0181] The drive unit includes a motor and a transmission mechanism. The pressing component is connected to the motor through the transmission mechanism, which can be a lead screw and nut mechanism, a cam mechanism, or a gear and rack mechanism.

[0182] In this embodiment, the first pressing component and the second pressing component are independently driven by a driving device, which employs a lead screw and nut mechanism. Specifically, as shown... Figures 18-21 As shown, the driving device includes a first motor 321, a first lead screw and nut mechanism 322, a second motor 323, and a second lead screw and nut mechanism 324. A bracket 302 extending upwards is fixedly mounted on the support plate 301, and both the first motor 321 and the second motor 323 are fixedly mounted on the bracket 302. The first pressing component is connected to the first motor 321 via the first lead screw and nut mechanism 322, and the second pressing component is connected to the second motor 323 via the second lead screw and nut mechanism 324. Specifically, the first lead screw and nut mechanism 322 is connected to the connecting seat 313 of the first pressing component, and the second lead screw and nut mechanism 324 is connected to the connecting seat 313 of the second pressing component.

[0183] The first motor 321 rotates in both directions, driving the connecting seat 313 of the first pressing assembly to move up and down through the first lead screw and nut mechanism 322, thereby causing the pressing heads 311 mounted thereon to move synchronously. The second motor 323 rotates in both directions, driving the connecting seat 313 of the second pressing assembly to move up and down through the second lead screw and nut mechanism 324, thereby causing the pressing heads 311 mounted thereon to move synchronously.

[0184] When the first motor 321 and the second motor 323 rotate in opposite directions, they drive the first pressing component and the second pressing component to move alternately up and down, pressing the first mixing chamber 121 and the second mixing chamber 122 respectively, so that the contents of the first mixing chamber 121 and the second mixing chamber 122 are mixed evenly. When the first motor 321 and the second motor 323 rotate in the same direction, they drive the first pressing component and the second pressing component to move synchronously upward or downward. When they move synchronously downward, they press the first mixing chamber 121 and the second mixing chamber 122 respectively, causing their contents to flow out.

[0185] The road closure mechanism is used to block the mixing fluid channel 124. The road closure mechanism includes road closure components, such as... Figures 19-21 As shown, the sealing assembly includes a pressure head 331 for pressing against the mixing fluid channel 124.

[0186] There are 1, 2, 3, 4, 5, 6 or more pressure heads 331. When there are multiple pressure heads 331, the multiple pressure heads 331 are distributed in parallel and at intervals.

[0187] The number of pressure heads 331 can be set according to the number of mixing fluid channels 124, specifically, the number of pressure heads 331 is less than the number of mixing fluid channels 124. Preferably, the number of mixing fluid channels 124 is an integer multiple of the number of pressure heads 331. In this embodiment, the number of pressure heads 331 is the same as the number of pressing heads 311, and the number of mixing fluid channels 124 is twice the number of pressure heads 331. This arrangement improves detection efficiency while making the pressing device more compact and smaller in size.

[0188] The sealing assembly may also include a pressure seat 332, which is located below the support plate 301. Each pressure head 331 is located at the bottom of the pressure seat 332. The action of the pressure seat 332 can drive the action of each pressure head 331 synchronously, thereby achieving simultaneous sealing of multiple mixed fluid channels 124.

[0189] like Figure 21 As shown, the sealing assembly may further include a heating element 335 and a temperature sensor 336 respectively disposed on the pressure seat 332. The pressure head 331 is made of aluminum alloy. The heating element 335 is used to heat the pressure head 331, so that when the pressure head 331 presses against the mixed fluid channel 124, it can melt and seal the upper membrane 1c and lower membrane 1b of the formed mixed fluid channel 124, thereby re-sealing the mixed fluid channel 124 and preventing the mixed fluid entering the detection unit from being sucked back. The temperature sensor 336 is used to monitor the heating temperature of the heating element 335 so that the heating element 335 heats the pressure head 331 to the set temperature.

[0190] The road closure mechanism also includes an elastic element 34, which is used to drive the road closure assembly to move downward in a direction that tends to approach the mixing fluid channel 124. The elastic element 34 is disposed between the support plate 301 and the pressure seat 332.

[0191] In this embodiment, a downwardly extending guide shaft is fixedly provided on the support plate 301, the pressing seat 332 is slidably provided on the guide shaft along the length extension direction of the guide shaft, and the elastic member 34 is sleeved on the outside of the guide shaft.

[0192] The sealing assembly has a highest position and a lowest position. When the sealing assembly is in the lowest position, the pressure head 331 presses against the mixing fluid channel 124. When the sealing assembly is in the highest position, the pressure head 331 moves away from the mixing fluid channel 124, and the elastic element 34 is in a compressed state.

[0193] The road closure mechanism also includes a locking mechanism, which locks the road closure components in the highest position. For example... Figure 18 and Figure 19As shown, the locking mechanism includes a locking member 35 slidably disposed on the support plate 301. In this embodiment, the sliding direction of the locking member 35 is perpendicular to the movement direction of the road-blocking assembly. When the locking mechanism is in the locked state, the locking member 35 is located on the movement path of the road-blocking assembly, thereby blocking the downward movement of the road-blocking assembly and preventing it from moving downward. When the locking mechanism is in the unlocked state, the locking member 35 leaves the movement path of the road-blocking assembly, and the road-blocking assembly can move downward to the lowest position under the drive of the elastic force of the elastic member 34.

[0194] The road-blocking assembly also includes an abutment rod 333, the lower end of which is fixedly mounted on the abutment seat 332. The abutment rod 333 is slidably mounted on the support plate 301, and a blocking part 334 is fixedly mounted on the upper end of the abutment rod 333. Figure 18 and Figure 19 As shown.

[0195] When the locking mechanism is in the locked state, the locking member 35 is located below the blocking part 334, and the adjacent end faces of the two abut against each other. When the locking mechanism is in the unlocked state, the locking member 35 moves away from below the blocking part 334.

[0196] The lower end of the locking member 35 is provided with an inclined surface 351 that slopes downwards and away from the movement path of the road sealing assembly. When the road sealing assembly moves upwards, the blocking part 334 can engage with the inclined surface 351, thereby driving the locking member 35 to slide away from the movement path of the road sealing assembly. That is, this locking mechanism is a one-way locking mechanism.

[0197] In this embodiment, the locking element 35 is an electromagnet. When the electromagnet is de-energized, the locking element 35 blocks the movement path of the road sealing assembly. When the electromagnet is energized, the locking element 35 moves away from the movement path of the road sealing assembly.

[0198] The road-closing mechanism and the pressing mechanism are located adjacent to each other, and a detachable connecting structure is provided between them. The connecting structure has a connected state and a disconnected state. When the connecting structure is in the connected state, the road-closing component can be driven to move upward by the pressing mechanism.

[0199] In this embodiment, the connecting structure includes a mating part disposed on one of the connecting seat 313 and the pressing seat 332, and a limiting member 3321 disposed on the other. When the limiting member 3321 engages with the mating part, the connecting structure is in a connected state. When the limiting member 3321 disengages from the mating part, the connecting structure is in a separated state.

[0200] The pressing assembly has an initial position. When the pressing assembly is in the initial position, the sealing assembly is in its highest position, and the limiting member 3321 engages with the mating part.

[0201] The pressing assembly reciprocates to press the mixing chamber, having a highest and a lowest position, with the initial position above the highest position of the pressing assembly's reciprocating motion. When the limiting member 3321 is mounted on the pressing seat 332 and the mating part is mounted on the connecting seat 313, when the sealing assembly is in the lowest position, the mating part is below the limiting member 3321 when the pressing assembly reciprocates to the highest position. When the limiting member 3321 is mounted on the connecting seat 313 and the mating part is mounted on the pressing seat 332, when the sealing assembly is in the lowest position, the limiting member 3321 is below the mating part when the pressing assembly reciprocates to the highest position. Thus, during the reciprocating motion of the pressing assembly pressing the mixing chamber, the pressing head 331 always presses against the mixing fluid channel 124. Only when the pressing assembly moves from the reciprocating position to the initial position will the mating part engage with the limiting member 3321, thereby connecting the structure and allowing the pressing assembly to drive the sealing assembly from the lowest position to the highest position.

[0202] In this embodiment, the limiting member 3321 is a screw fixedly mounted on the pressing seat 332, and the mating part is a recessed part mounted on the connecting seat 313 that mates with the screw.

[0203] The pressing mechanism is also used to press the premixing chamber to mix the sample with the reagent in the premixing chamber and discharge the mixed fluid into the dispensing chamber 114. At this time, the first pressing component and the second pressing component respectively press the first premixing chamber and the second premixing chamber.

[0204] When the pressing mechanism presses the premixing chamber, the blocking mechanism presses against the premixed fluid channel 164 to block the premixed fluid channel 164. The structure of the blocking mechanism may be the same as or different from that of the road-blocking mechanism.

[0205] The blocking mechanism includes a blocking assembly for pressurizing the premixed fluid channel 164. The blocking assembly is disposed on a connecting seat 313 that is not connected to the sealing assembly, and is capable of sliding up and down relative to the connecting seat 313. Figures 18-21 As shown, the blocking assembly includes a blocking head 361 and a blocking rod 362. The blocking head 361 is made of rubber material and is disposed at the lower end of the blocking rod 362, which is slidably mounted on the connecting seat 313. The number of blocking heads 361 is determined according to the number of premixed fluid channels 164. In this embodiment, the number of blocking heads 361 is the same as the number of premixed fluid channels 164.

[0206] The blocking mechanism also includes an elastic element 37, which is used to drive the blocking assembly downward in a direction that tends to move closer to the premixed fluid channel 164. The elastic element 37 is disposed between the support plate 301 and the blocking assembly.

[0207] The blocking assembly has a highest position and a lowest position. When the blocking assembly is in the lowest position, the blocking head 361 presses against the premixed fluid channel 164. When the blocking assembly is in the highest position, the blocking head 361 moves away from the premixed fluid channel 164, and the elastic element 37 is compressed.

[0208] like Figure 18 and Figure 19 As shown, the blocking mechanism also includes a locking mechanism 38, which locks the blocking assembly in the highest position. A blocking part 363 is also provided on the upper part of the blocking rod 362. The locking method of the blocking part 363 and the locking structure 38 is the same as that in the road closure mechanism, and will not be repeated here.

[0209] In this embodiment, the elastic element 37 is sleeved on the outside of the blocking rod 362.

[0210] The blocking mechanism and the pressing mechanism are also arranged adjacent to each other, and a detachable connecting structure is provided between them. When the connecting structure is in the connected state, the pressing mechanism drives the blocking component to move upward. The connecting structure between the blocking mechanism and the pressing mechanism can be the same as or different from the connecting structure between the road-blocking mechanism and the pressing mechanism. In this embodiment, the two are based on the same principle, differing only in their structural form.

[0211] The pressing mechanism is also used to press the reagent storage chamber to push the fluid in the reagent storage chamber into the fourth detection chamber 17. At this time, the first pressing component and the second pressing component respectively press the first reagent storage chamber and the second reagent storage chamber.

[0212] When the number of pressing heads 311 corresponding to the mixing chambers is set to be multiple, when the premixing chamber and the reagent storage chamber are pressed by the pressing mechanism, the position of a set of pressing components can be made to correspond to the position of the premixing chamber and the reagent storage chamber. During the pressing process, the pressing head 311 will not apply force to the consumables through the retraction of the pressing rod 312, thereby avoiding damage to the consumables or pressing components.

[0213] Example 4

[0214] This embodiment provides another type of pressing device, which is also used to press the aforementioned enclosed chip consumable.

[0215] In this embodiment, the first pressing component and the second pressing component are simultaneously driven by the same driving device, which adopts a cam mechanism.

[0216] Specifically, such as Figures 22-24As shown, the driving device includes a motor 41 and a rotatably mounted cam 42. The motor 41 is connected to the camshaft 43 of the cam 42. The first pressing component and the second pressing component are respectively engaged with the outer contour surface of the cam 42. The rotation of the cam 42 drives the first pressing component and the second pressing component to move downward.

[0217] like Figure 24 As shown, the driving device also includes an elastic element 44, which is respectively disposed between the first pressing assembly and the support plate 301 and the second pressing assembly and the support plate 301. The elastic force of the elastic element 44 is used to drive the first pressing assembly and the second pressing assembly to move upward in a direction that tends to move away from the mixing chamber.

[0218] In this way, the reciprocating motion of the first pressing component and the second pressing component, as well as their synchronous upward or downward motion, are achieved through the combined action of the cam 42 and the elastic element 44.

[0219] like Figure 25 As shown, the outer contour surface of the cam 42 includes a first region 421. The first region 421 includes a central abutment portion 4211 located at the center, and a first abutment portion 4212 and a second abutment portion 4213 respectively disposed on opposite sides of the central abutment portion 4211. On the first abutment portion 4212 and the second abutment portion 4213, along the direction away from the central abutment portion 4211, the distance from each point on the first abutment portion 4212 and the second abutment portion 4213 to the camshaft 43 gradually increases. Preferably, the first abutment portion 4212 and the second abutment portion 4213 are symmetrically arranged.

[0220] When the fluid in the mixing chamber is mixed, the cam 42 rotates to the first region 421 facing the pressing assembly. The first abutting part 4212 abuts against the first pressing assembly, and the second abutting part 4213 abuts against the second pressing assembly. When the cam 42 rotates forward, it drives the first pressing assembly downward to press the first mixing chamber 121, causing the fluid to flow from the first mixing chamber 121 to the second mixing chamber 122. At this time, the second pressing assembly moves upward under the elastic force of the elastic member 44. When the cam 42 rotates in the reverse direction, the second abutting part 4213 abuts against the second pressing assembly, causing it to move downward to press the second mixing chamber 122, causing the fluid to flow from the second mixing chamber 122 to the first mixing chamber 121. At this time, the first pressing assembly moves upward under the elastic force of the elastic member 44.

[0221] In this way, by rotating the cam 42 in both directions within a certain angle range, the first pressing component and the second pressing component can move up and down alternately to press the first mixing chamber 121 and the second mixing chamber 122 respectively, thereby achieving the mixing of fluids.

[0222] like Figure 25As shown, the outer contour surface of each cam 42 also includes a second region 422 sequentially connected to the first region 421. In this embodiment, the outer wheel surface of the cam 42, except for the first region 421, is entirely within the second region 422. The outer contour surface of the cam 42 within the second region 622 is arc-shaped, meaning that each point on the outer contour surface is at the same distance from the camshaft 43. The ends of the first abutment portion 4212 and the second abutment portion 4213 away from the central abutment portion 4211 are sequentially connected to the second region 422.

[0223] When the fluid in the mixing chamber is discharged, the cam 42 rotates to the second region 622 facing the pressing component. The cam 42 is located on the outer contour surface of the second region 422 and simultaneously presses against the first pressing component and the second pressing component. When the cam 42 rotates within a certain angle range, it can simultaneously cause the first pressing component and the second pressing component to move downward and press the first mixing chamber 121 and the second mixing chamber 122 respectively.

[0224] One or more cams 42 are provided for the mixing chamber. When there are multiple cams 42, the multiple cams 42 are set on the same camshaft 43 and driven by the same motor 41 to rotate synchronously. This can press multiple mixing chambers at the same time, which can improve efficiency, make the structure compact and small in size, and save costs.

[0225] In this embodiment, in both the first and second pressing assemblies, a mating part 314 that engages with the cam 42 is provided at the upper end of the pressing rod 312. Preferably, the mating part 314 is rotatably mounted on the pressing rod 312. Figure 26 As shown. By rotating the mating part 314, the frictional resistance when the cam 42 rotates can be reduced.

[0226] In the road-blocking mechanism of this embodiment, the road-blocking component may not include the pressure seat 332, and the pressure head 331 is directly disposed at the lower end of the pressure rod 333.

[0227] like Figures 22-24 As shown, the sealing mechanism in this embodiment also includes a motor 451 and a transmission mechanism 452. The sealing assembly is connected to the motor 451 through the transmission mechanism 452 to drive the sealing assembly to move downwards and block the mixing fluid channel 124. The transmission mechanism 452 can be a screw and nut mechanism or a gear and rack mechanism. In this embodiment, the transmission mechanism 452 adopts a screw and nut mechanism.

[0228] A connecting plate 453 is fixedly mounted on the screw and nut mechanism, and each sealing component is slidably mounted on the connecting plate 453. The pressing mechanism is mounted on the connecting plate 453. When the connecting plate 453 is driven by the motor 452 and the screw and nut mechanism to move each sealing component downward, it drives each pressing component to move downward synchronously. This reduces the travel distance of the cam 42 driving the first and second pressing components, thereby reducing the structural size of the cam 42 and consequently reducing the structural size of the pressing device.

[0229] like Figures 22-24 As shown, an elastic element 46 can be provided between the connecting plate 453 and the pressing head 331. When the sealing assembly moves downward and the pressing head 331 presses against the mixing fluid channel 124, the elastic element 46 can buffer the downward movement of the pressing head 331 and prevent the pressing head 331 from moving downward too fast and damaging the consumables.

[0230] like Figures 22-24 As shown, the connection structure in this embodiment includes a paddle 471 fixedly mounted on the camshaft 43 and a pressure plate 472 fixedly mounted on the upper end of the pressure rod 333. When the cam 42 rotates to a set angle, the paddle 471 rotates to the underside of the pressure plate 472 and abuts against the pressure plate 472, thereby putting the connection structure in a connected state. When the cam 42 continues to rotate, the paddle 471 acts on the pressure plate 472, causing it to drive the sealing assembly upwards, causing the pressure head 331 to leave the mixing fluid channel 124.

[0231] The working principle of this pressing device is as follows:

[0232] (1) The motor 451 starts, driving the screw and nut mechanism to move the connecting plate 453 downward, which in turn moves the sealing component downward until the pressing head 331 presses against the mixing fluid channel 124, thereby blocking the mixing fluid channel 124. During this process, the pressing mechanism moves downward synchronously with the connecting plate 453, but the pressing component does not press the mixing chamber.

[0233] (2) The motor 41 rotates, causing the cam 42 to rotate so that the first region 421 faces the first pressing component and the second pressing component, and the cam 42 rotates in both directions in this state, so that the first pressing component and the second pressing component move up and down alternately, thereby alternately pressing the first mixing chamber 121 and the second mixing chamber 122, so that the fluid flows back and forth between the first mixing chamber 121 and the second mixing chamber 122 for mixing.

[0234] (3) Rotate the cam 42 so that the second region 422 faces the first pressing component and the second pressing component. The first pressing component and the second pressing component are at the same height. When the cam 42 continues to rotate, it drives the first pressing component and the second pressing component to move downward synchronously, so that the fluid in the first mixing chamber 121 and the second mixing chamber 122 flows out and enters the mixing fluid channel 124, and the sealing structure 1e in the mixing fluid channel 124 is destroyed by the fluid pressure.

[0235] (4) After the protrusion 42 continues to rotate at a certain angle, the paddle 471 rotates to the bottom of the pressure plate 472 and abuts against the pressure plate 472, so that the connecting structure is in the connected state. When the cam 42 continues to rotate, the paddle 471 exerts an upward force on the pressure plate 472, causing the sealing assembly to move upward as a whole, and the pressing head 331 leaves the mixing fluid channel 124, so that the mixing fluid channel 124 is in a non-blocked state. At this time, the first pressing assembly and the second pressing assembly are still at the same height.

[0236] (5) The motor 451 starts, driving the lead screw and nut mechanism to move the connecting plate 453 downward, which drives the first pressing component and the second pressing component to move downward synchronously. The first pressing component and the second pressing component press the first mixing chamber 121 and the second mixing chamber 122 respectively, thereby squeezing the fluid in the first mixing chamber 121 and the second mixing chamber 122 out of the first mixing chamber 121 and the second mixing chamber 122, and entering the detection unit through the mixing fluid channel 124.

[0237] (6) Reset the pressing mechanism and the road sealing mechanism.

[0238] Example 5

[0239] This embodiment provides a detection device, which includes the pressing device of Embodiment 3 or Embodiment 4. This embodiment takes the pressing device of Embodiment 3 as an example. Figure 27 As shown, the testing equipment also includes a base 5, a transfer mechanism, a pin mechanism 7, and a testing mechanism.

[0240] like Figure 27As shown, a support plate 301 is fixedly mounted above the base 5, and a transfer mechanism is mounted on the base 5, located below the support plate 301. The transfer mechanism includes an X-axis drive platform 61 and a Y-axis drive platform 62. The Y-axis drive platform 62 is mounted on the base 5, and the X-axis drive platform 61 is mounted on the Y-axis drive platform 62. Consumables are mounted on the X-axis drive platform 61, located below the support plate 301. Specifically, the X-axis drive platform 61 includes a tray 611, and consumables are fixedly mounted within the tray 611. The X-axis drive platform 61 and the consumables are driven synchronously along the Y-axis direction by the Y-axis drive platform 62 to adjust the position of the consumables in the Y-axis direction. The consumables are driven to move along the X-axis direction by the X-axis drive platform 61 to adjust their position in the X-axis direction.

[0241] like Figure 27 As shown, the ejector mechanism 7 is mounted on the base 5 and located below the support plate 301. The detection device achieves the pushing of samples and fluids through the cooperation of the ejector mechanism 7 and the transfer mechanism.

[0242] like Figure 28 and Figure 29 As shown, the ejector mechanism includes an ejector seat 71 and a first ejector assembly, a second ejector assembly, and a third ejector assembly respectively disposed on the ejector seat 71.

[0243] The first ejector pin assembly is used to push the sample into the main body. For the consumable of Example 1, the first ejector pin assembly is used to push the sample in the delivery tube assembly 21 into the premixing chamber and the fourth detection chamber 17. For the consumable of Example 2, the first ejector pin assembly is used to push the fluid in the dilution tube assembly 22 into the dispensing chamber and push the sample in the delivery tube assembly 21 into the fourth detection chamber 17. The first ejector pin assembly includes a first ejector pin 72 disposed on the ejector pin seat 71 and an elastic member 73 disposed between the ejector pin seat 71 and the first ejector pin 72, such as... Figure 30 As shown.

[0244] The second ejector assembly is used to push the plug 116 in the overflow cavity 115 so that the plug 116 in the overflow cavity 115 blocks the inlet of the overflow cavity 115. The second ejector assembly includes a second ejector 74 disposed on the ejector seat 71 and an elastic member 73 disposed between the ejector seat 71 and the second ejector 74.

[0245] The third ejector assembly is used to push the plug 116 in the metering chamber 111, thereby pushing all the fluid in the metering chamber 111 into the mixing chamber to ensure that a metered amount of fluid enters the mixing chamber. Figure 31 As shown, the third ejector assembly includes a third ejector 75 disposed on the ejector seat 71 and an elastic member 73 disposed between the ejector seat 71 and the third ejector 75. One or more third ejector assemblies are provided, each corresponding to a metering cavity 111.

[0246] Elastic elements 73 are provided between the first ejector pin 72 and ejector pin seat 71, the second ejector pin 74 and ejector pin seat 71, and the third ejector pin 75 and ejector pin seat 71. This ensures that when each ejector pin pushes the plug 116 or sealing piston 213 / 223 to the bottom, even with some excessive movement, the ejector pins will not be damaged or the motor will lose steps, thus ensuring the reliability and motion accuracy of the equipment. Furthermore, a certain amount of excessive movement can also push the plug 116 or sealing piston 213 / 223 in the channel to the very bottom, ensuring that the metered fluid is fully propelled in, guaranteeing accurate metering.

[0247] In this embodiment, corresponding to the consumable structure, the second ejector pin 731 and the third ejector pin 741 are disposed on the same side of the ejector pin seat 71, and the length of the second ejector pin 731 is greater than the length of the third ejector pin 741. Thus, when the plug 116 in the overflow cavity 115 is pushed by the second ejector pin assembly to block the inlet of the overflow cavity 115, the third ejector pin 75 can always maintain a distance from the consumable, and the third ejector pin 75 is in a non-working state. When the plug 116 in the overflow chamber 115 blocks the inlet of the overflow chamber 115, the consumable is moved by the transfer mechanism to the position of the third ejector assembly corresponding to each metering chamber 111. The position of the second ejector assembly is staggered from that of the overflow chamber 115. In this way, when the third ejector assembly pushes the plug 116 in the metering chamber 111 to push the fluid in the metering chamber 111 to the mixing chamber, the second ejector assembly is located outside the consumable and is in an inactive state. This ensures that the plug 116 in the overflow chamber 115 always blocks the inlet of the overflow chamber 115, thereby preventing the fluid in the metering chamber 111 from entering the overflow chamber 115 when the third ejector assembly pushes the plug 116 in the metering chamber 111. This would not guarantee that all the fluid in the metering chamber 111 is pushed into the mixing chamber, and would not achieve quantitative fluid delivery.

[0248] like Figure 27 As shown, the detection mechanism is mounted on the support plate 301. Figure 32 and Figure 33 As shown, the detection mechanism includes a microscopic imaging component 81, which is used to take pictures of the fluid in the first detection chamber 131. The microscopic imaging component 81 has a lens 811 and can be moved in the vertical direction to adjust the distance between the lens 811 and the first detection chamber 131 within a set range to obtain a clear image.

[0249] The detection mechanism also includes a drive mechanism, which drives the microscopic imaging component 81 to move up and down to adjust its position. The detection device also includes a fixed base 83 fixedly mounted on the support plate 301. The drive mechanism includes a motor 821 mounted on the fixed base 83 and a reducer 822 connected to the motor 821. The microscopic imaging component 81 is connected to the reducer. Alternatively, the microscopic imaging component 81 can be connected to the reducer 822 via a transmission mechanism, which can be a lead screw and nut mechanism, a gear and rack mechanism, etc.

[0250] In one embodiment, the reducer 822 is a synchronous belt reducer, which includes a housing and a synchronous belt disposed within the housing. The photomicrography component 81 is connected to the synchronous belt. When the motor 821 starts, it drives the synchronous belt connected to the photomicrography component 81 to move upward or downward, thereby driving the photomicrography component 81 to move synchronously. The structure of the synchronous belt reducer completely eliminates gaps after the synchronous belt is tensioned, achieving a true zero-backlash structure. This ensures error-free movement of the photomicrography component 81, thereby precisely controlling its position and improving imaging results.

[0251] The drive mechanism in this embodiment also includes a constant force spring connected between the housing and the microscope imaging assembly 81. The constant force spring balances the weight of the microscope imaging assembly 81, reducing the force required for the synchronous belt to drive the microscope imaging assembly 81 up and down, resulting in smoother and more precise movement of the microscope imaging assembly 81.

[0252] In another embodiment, the reducer 822 is a gear reducer, and the transmission mechanism includes a rack 823 mounted on a fixed base 83 and a gear 824 meshing with the rack 821. The gear 824 is mounted on the output shaft of the reducer 822, and the microscopic imaging assembly 81 is fixedly connected to the rack 823. Figure 33 As shown. When the motor 821 starts, it drives the gear 824 to rotate through the high transmission ratio reducer 822, which causes the rack 823 meshing with it to move up and down, thereby driving the microscope imaging component 81 to move.

[0253] like Figure 33 As shown, the testing mechanism also includes a camera 841 and a supplementary light 842, which are used during the immunoassay test. The camera 841 and the supplementary light 842 are fixedly mounted on the mounting base 83.

[0254] The testing facility also includes a light source module for hemoglobin detection. After the fluid enters the third detection chamber 15, detection can be performed through the light source module. For example... Figure 34As shown, the light source module includes a light source mounting base 851, an LED light source 852, a receiver mounting base 853, and a receiver 854. The light source mounting base 851 is used to mount the LED light source 852, and the receiver mounting base 853 is used to mount the receiver 854. Both the light source mounting base 851 and the receiver mounting base 853 are fixedly mounted on the tray 611, located on opposite sides of the third detection chamber 15. During hemoglobin detection, the light emitted by the LED light source 852 passes through the cavity of the third detection chamber 15 and reaches the receiver 854, thereby detecting the absorbance value of hemoglobin. The receiver 854 then transmits the data results to the detection terminal.

[0255] The testing equipment also includes a heating monitoring module, which is always operational to ensure that the fluid in the consumables remains at the set temperature during the testing process. Figure 35 As shown, the heating monitoring module includes a heating element 91 and a sensor 92 attached to the back of the tray 611. The heating element 91 heats the tray 611, transferring heat to the consumables. The sensor 92 monitors the temperature of the tray 611. If the temperature exceeds the set temperature, the heating element 91 stops heating. If the temperature is below the set temperature, the heating element 91 heats up, thus ensuring that the fluid in the consumables reaches the set temperature.

[0256] Taking the consumables of Example 1 and the pressing device of Example 3 as examples, the method of testing using this testing device is described as follows:

[0257] (1) After collecting samples, the delivery tube group 21 is installed one by one at the sample inlet 10, and the consumables are installed on the tray 611.

[0258] (2) Before the equipment starts running, check that the first motor 321 and the second motor 322 are in the initial position, the locking mechanism and the locking stop mechanism are in the locked state, so that the road blocking component and the blocking component are in the highest position.

[0259] (3) The consumables are driven to move along the X-axis and Y-axis directions respectively by the X-axis drive platform 61 and the Y-axis drive platform 62 until the position of the first ejector assembly corresponds one-to-one with the position of the delivery tube group 21. The consumables are driven to move along the Y-axis direction and toward the first ejector assembly by the Y-axis drive platform 62. The first ejector 72 abuts against the sealing piston 213 in the delivery tube group 21, pushing the sealing piston 213 to move, thereby pushing the sample in the delivery tube group 21 from the sample inlet 10 to the premixing chamber and the fourth detection chamber 17.

[0260] (4) Drive the consumable along the X-axis and Y-axis directions respectively by the X-axis drive platform 61 and the Y-axis drive platform 62, so that the positions of the pressing mechanism and the blocking mechanism correspond to the positions of the pre-dilution area on the consumable.

[0261] (5) The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to move downward to the set position, so that the locking mechanism is unlocked, and the blocking component moves downward to the lowest position under the action of the elastic force of the elastic member 37, and presses against the premixed fluid channel 164 to block the premixed fluid channel 164.

[0262] (6) The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to continue moving downward to the set position. The first motor 321 and the second motor 322 rotate in opposite directions, causing the first pressing component and the second pressing component to alternately move up and down to press the first premixing chamber 161 and the second premixing chamber 162, so that the sample is mixed with the reagent in the premixing chamber.

[0263] (7) After the contents in the premixing chamber are mixed, the first pressing component and the second pressing component are at the same height. The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to move downward synchronously, pressing the first premixing chamber 161 and the second premixing chamber 162 respectively to make the fluid part flow out, thereby destroying the sealing structure 1e in the premixed fluid channel 164.

[0264] (8) The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to move upward synchronously until the connecting structure is in the connected state, driving the blocking component to move upward synchronously until it reaches the highest position, and the locking mechanism locks the blocking component in the highest position. The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to move downward synchronously, pressing the first premixing chamber 161 and the second premixing chamber 162 respectively to make the fluid flow into the distribution chamber 114, and after filling each metering chamber 111, the first pressing component and the second pressing component are reset to the initial position.

[0265] (9) The consumable is driven to move along the X-axis and Y-axis directions respectively by the X-axis drive platform 61 and the Y-axis drive platform 62, so that the position of the second ejector assembly corresponds to the position of the overflow cavity 115. The consumable is driven to move along the X-axis direction and toward the second ejector assembly by the X-axis drive platform 62, and the second ejector 74 pushes the plug 116 in the overflow cavity 115 to move and block the inlet of the overflow cavity 115.

[0266] (11) The consumable is driven by the X-axis drive platform 62 to move along the X-axis and away from the second ejector assembly, causing the second ejector 74 to exit the overflow chamber 115. The consumable is driven by the X-axis drive platform 61 and the Y-axis drive platform 62 to move along the X-axis and Y-axis respectively, so that the position of the third ejector assembly corresponds one-to-one with the position of the metering chamber 111. The consumable is driven by the X-axis drive platform 62 to move along the X-axis and towards the third ejector assembly, causing the third ejector 75 to push the plug 116 in the metering chamber 111 to move, so that the fluid in the metering chamber 111 breaks through the sealing structure 1e in the sample outlet channel 112 and enters the mixing chamber.

[0267] (12) Drive the consumables along the X-axis and Y-axis directions respectively by the X-axis drive platform 61 and the Y-axis drive platform 62, so that the positions of the pressing mechanism and the sealing mechanism correspond to the positions of the mixing area on the consumables.

[0268] (13) The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to move downward to the set position, so that the locking mechanism is unlocked, and the sealing component moves downward to the lowest position under the action of the elastic force of the elastic member 34, and the pressing head 331 presses against the mixing fluid channel 124 to block the mixing fluid channel 124.

[0269] (14) The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to continue moving downward to the set position. The first motor 321 and the second motor 322 rotate in opposite directions, causing the first pressing component and the second pressing component to alternately move up and down to press the first mixing chamber 121 and the second mixing chamber 122 to mix the contents in the mixing chamber.

[0270] (15) After the contents in the mixing chamber are mixed, the first pressing component and the second pressing component are at the same height. The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to move downward synchronously, pressing the first mixing chamber 121 and the second mixing chamber 122 respectively to make the fluid part flow out, thereby destroying the sealing structure 1e in the mixing fluid channel 124.

[0271] (15) The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to move upward synchronously until the connecting structure is in the connected state, driving the sealing component to move upward synchronously until it reaches the highest position, and the locking mechanism locks the sealing component in the highest position. The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to move downward synchronously, pressing the first mixing chamber 121 and the second mixing chamber 122 respectively, so that the fluid flows into the first detection chamber 131, the second detection chamber 142 or the third detection chamber 15 respectively.

[0272] (16) Drive the consumable along the X-axis and Y-axis directions respectively by the X-axis drive platform 61 and the Y-axis drive platform 62 so that the position of the pressing component corresponds to the position of the reagent storage cavity.

[0273] (17) The first motor 321 and the second motor 322 are started, driving the first pressing component and the second pressing component to move downward, pressing the first reagent storage chamber 182 and the second reagent storage chamber 183 respectively, and pushing the fluid in the reagent storage chamber onto the test strip in the fourth detection chamber 17.

[0274] During the above testing process, when the fluid flows out of the premixing chamber and the mixing chamber, the sealing structure 1e in the premixing fluid channel 164 and the mixing fluid channel 124 fails first under fluid pressure, and then the blocking component and the sealing component leave the corresponding channel. This operation can prevent the upper clear liquid in the premixing chamber and the mixing chamber from going out first, which would cause uneven cell distribution in the channel. It can also prevent the fluid from rushing out rapidly due to the failure of the sealing structure 1e, which would cause uneven cell distribution.

[0275] When the blocking component presses against the premixed fluid channel 164 and the sealing component presses against the mixed fluid channel 124, their positions on the channel are respectively located on the side of the sealing structure 1e away from the premixing chamber and the mixing chamber, and there is a gap between the pressing position and the sealing structure 1e, preferably 0.5 to 1 mm. This ensures that the sealing structure 1e is not damaged during the mixing process, and at the same time, the pressure of the fluid flowing out when the blocking component presses against the premixed fluid channel 164 and the sealing component presses against the mixed fluid channel 124 can destroy the sealing structure 1e.

[0276] During the process of fluid entering the first detection chamber 131, the second detection chamber 142, and the third detection chamber 15 for detection, the pressure head 331 of the sealing assembly can be pressed against the mixed fluid channel 124 again, and the pressure head 331 can be heated to partially bond the upper membrane 1c and the lower membrane 1b on the mixed fluid channel 124 to form a sealing structure 1e, so that the mixed fluid channel 124 is in a blocked state to avoid fluid backflow affecting the detection results during the detection process.

[0277] During detection by the first detection unit, the fluid in the mixing chamber can be injected into the first detection chamber 131 multiple times for detection. After each detection, the mixing chamber is pressed to fill the first detection chamber 131 with new fluid, and the original fluid in the first detection chamber 131 is discharged into the waste liquid chamber 133. This allows for the acquisition of more cells, thereby improving detection accuracy.

[0278] The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly. They should not be construed as limiting the scope of protection of the present invention. All equivalent changes or modifications made in accordance with the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A pressing device, characterized in that: For pressing closed-type chip consumables, the consumables include one or more deformable mixing chambers under external force, and mixing fluid channels respectively connected to the inlet and / or outlet of one or more of the mixing chambers, the pressing device comprising: A pressing mechanism for pressing the mixing chamber, the pressing mechanism including a pressing assembly capable of reciprocating along the pressing direction, the pressing assembly including a pressing head that presses against the mixing chamber; A blocking mechanism for blocking the mixed fluid passage, the blocking mechanism including a blocking assembly including a pressing head for pressing against the mixed fluid passage.

2. The pressing device according to claim 1, characterized in that: The number of pressing heads is less than the number of mixing chambers. Preferably, the number of mixing chambers is an integer multiple of the number of pressing heads included in the pressing assembly.

3. The pressing device according to claim 1, characterized in that: The number of pressing heads is 1, 2, 3, 4, 5, 6 or more. When there are multiple pressing heads, the multiple pressing heads are distributed in parallel and at intervals.

4. The pressing device according to claim 3, characterized in that: The pressing assembly also includes an elastic element for driving the pressing head to move in a direction that tends to move away from the mixing chamber; And / or, the pressing assembly further includes a pressing rod, the elastic element is integrated into the pressing rod to enable the pressing rod to extend and retract in the pressing direction, and the pressing head is disposed on the pressing rod; And / or, each of the pressing components further includes a connecting seat, on which the pressing rod is disposed.

5. The pressing device according to claim 1, characterized in that: The pressing mechanism further includes a driving device for driving the pressing assembly to move. The driving device includes a motor and a transmission mechanism. The pressing assembly is connected to the motor via the transmission mechanism, which is a lead screw and nut mechanism, a cam mechanism, or a rack and pinion mechanism; and / or, The sealing mechanism and the pressing mechanism are arranged adjacent to each other, and a separable connection structure is provided between them. The connection structure has a connected state and a separated state. When the connection structure is in the connected state, the pressing mechanism drives the sealing assembly to move away from the mixing chamber.

6. The pressing device according to any one of claims 1 to 4, characterized in that: Each of the mixing chambers includes a first mixing chamber and a second mixing chamber that are interconnected. The pressing assembly includes a first pressing assembly for pressing the first mixing chamber and a second pressing assembly for pressing the second mixing chamber. The pressing mechanism also includes a driving device for driving the first pressing assembly and the second pressing assembly to move. The first pressing assembly and the second pressing assembly are driven independently by the driving device, or the first pressing assembly and the second pressing assembly are driven simultaneously by the driving device and move in the same or opposite directions.

7. The pressing device according to claim 6, characterized in that: The driving device includes a first motor, a first lead screw and nut mechanism, a second motor, and a second lead screw and nut mechanism. The first pressing component is connected to the first motor through the first lead screw and nut mechanism, and the second pressing component is connected to the second motor through the second lead screw and nut mechanism.

8. The pressing device according to claim 6, characterized in that: The driving device includes a motor, one or more rotatably arranged cams, the cams being correspondingly arranged to the mixing chamber, the one or more cams being arranged on the same camshaft, the motor being connected to the camshaft, the first pressing component and the second pressing component respectively abutting and cooperating with the outer contour surface of the cam, and the driving device further includes an elastic element connected to the pressing component for driving the pressing component to move in a direction tending away from the mixing chamber.

9. The pressing device according to claim 8, characterized in that: The outer contour surface of the cam includes a first region, which includes a central abutment located at the center, a first abutment and a second abutment respectively disposed on opposite sides of the central abutment, and the distance from each point on the first abutment and the second abutment to the camshaft gradually increases along the direction away from the central abutment. The outer contour surface of the cam also includes a second region sequentially connected to the first region, and within the second region, all points on the outer contour surface of the cam are at the same distance from the camshaft.

10. The pressing device according to claim 8, characterized in that: A paddle is fixedly mounted on the camshaft. When the cam rotates to a set angle, the paddle engages with the sealing assembly to drive the sealing assembly away from the mixing fluid channel as the cam rotates.

11. The pressing device according to claim 1, characterized in that: The number of pressure heads is less than the number of mixing fluid channels. Preferably, the number of mixing fluid channels is an integer multiple of the number of pressure heads; and / or, the number of pressure heads is 1, 2, 3, 4, 5, 6 or more. When there are multiple pressure heads, the multiple pressure heads are distributed in parallel and spaced apart; and / or, the number of pressure heads is the same as the number of pressing heads.

12. The pressing device according to claim 1, characterized in that: The road sealing assembly also includes a pressure seat, the pressure head is disposed on the pressure seat, and the road sealing assembly also includes a heating element disposed on the pressure seat for heating the pressure head and a temperature sensor disposed on the pressure seat for monitoring the heating temperature of the heating element.

13. The pressing device according to claim 1, characterized in that: The sealing mechanism further includes an elastic element for driving the sealing assembly in a direction that tends to move toward the mixing fluid channel. The sealing assembly has a highest position and a lowest position. When the sealing assembly is in the lowest position, the pressure head presses against the mixing fluid channel. When the sealing assembly is in the highest position, the pressure head moves away from the mixing fluid channel, and the elastic element is in a compressed state.

14. The pressing device according to claim 13, characterized in that: The road-blocking mechanism further includes a locking mechanism for locking the road-blocking component at its highest position. The locking mechanism includes a slidably disposed locking member. When the locking mechanism is in the locked state, the locking member is located on the movement path of the road-blocking component. When the locking mechanism is in the unlocked state, the locking member leaves the movement path of the road-blocking component.

15. The pressing device according to claim 14, characterized in that: The lower end of the locking member is provided with an inclined surface that is inclined toward the direction of the mixing chamber and away from the movement path of the sealing assembly. When the sealing assembly moves away from the mixing fluid channel, the sealing assembly can be matched with the inclined surface to drive the locking member to slide away from the movement path of the sealing assembly.

16. The pressing device according to claim 14, characterized in that: The locking element is an electromagnet. When the electromagnet is de-energized, the locking element blocks the movement path of the road-blocking assembly. When the electromagnet is energized, the locking element moves away from the movement path of the road-blocking assembly.

17. The pressing device according to claim 13, characterized in that: One of the pressing component and the road-blocking component is provided with a mating part, and the other is provided with a limiting member. The pressing component has an initial position. When the pressing component is in the initial position, the road-blocking component is in the highest position. The limiting member is connected to the mating part.

18. The pressing device according to claim 17, characterized in that: The pressing component has a highest position and a lowest position when it reciprocates to press the mixing chamber, and the initial position is located above the highest position of the pressing component during reciprocating motion; When the limiting member is disposed on the pressing seat and the mating part is disposed on the connecting seat, when the pressing component reciprocates to the highest position, the mating part is located below the limiting member; When the limiting member is disposed on the connecting seat and the mating part is disposed on the pressing seat, when the pressing component reciprocates to the highest position, the limiting member is located below the mating part.

19. The pressing device according to claim 1, characterized in that: The road-blocking mechanism also includes a motor and a transmission mechanism. The road-blocking assembly is connected to the motor through the transmission mechanism to drive the road-blocking assembly to move toward the mixing fluid channel. The transmission mechanism is a lead screw and nut mechanism or a gear and rack mechanism.

20. The pressing device according to claim 1, characterized in that: The consumable is also provided with a premixing chamber that can be deformed under external force and a premixed fluid channel connected to the inlet and / or outlet of the premixing chamber. The pressing device also includes a blocking mechanism, which includes a blocking component for pressing against the premixed fluid channel.

21. The pressing device according to claim 20, characterized in that: The blocking mechanism further includes an elastic member for driving the blocking assembly in a direction that tends to move toward the premixed fluid channel. The blocking assembly has a highest position and a lowest position. When the blocking assembly is in the lowest position, the blocking assembly presses against the premixed fluid channel. When the blocking assembly is in the highest position, the blocking assembly moves away from the premixed fluid channel, and the elastic member is in a compressed state. The blocking mechanism further includes a locking mechanism for locking the blocking component at its highest position. The locking mechanism includes a slidably disposed locking member. When the locking mechanism is in a locked state, the locking member is located on the movement path of the blocking component. When the locking mechanism is in an unlocked state, the locking member leaves the movement path of the blocking component. The blocking mechanism is located adjacent to the pressing mechanism, and a separable connecting structure is provided between them. The connecting structure has a connected state and a separated state. When the connecting structure is in the connected state, the pressing mechanism drives the blocking component to move away from the mixing chamber.

22. A testing device, characterized in that: It has a pressing device as described in any one of claims 1 to 21 and a pusher mechanism for pushing the sample into the mixing chamber.

23. The testing equipment according to claim 22, characterized in that: The consumable includes a main body, on which the mixing chamber and the mixing fluid channel are both disposed. The main body also includes a sample inlet, a dispensing chamber, one or more metering chambers, and an overflow chamber. The sample inlet, the one or more metering chambers, and the overflow chamber are all connected to the dispensing chamber. Each of the one or more metering chambers is connected to one or more of the mixing chambers. A plug is slidably disposed within each of the one or more metering chambers and the overflow chamber. The consumable also includes a delivery tube assembly disposed at the sample inlet. The ejector mechanism includes: Ejector seat; A first ejector assembly is used to push the sample in the delivery tube group into the body. The first ejector assembly includes a first ejector pin disposed on the ejector pin seat and an elastic member disposed between the ejector pin seat and the first ejector pin. The second ejector assembly is used to push the plug in the overflow cavity to block the inlet of the overflow cavity. The second ejector assembly includes a second ejector pin disposed on the ejector pin seat and an elastic member disposed between the ejector pin seat and the second ejector pin. One or more third ejector pin assemblies are used to push the plug in the metering chamber to push fluid into the mixing chamber. The third ejector pin assembly includes a third ejector pin disposed on the ejector pin seat and an elastic member disposed between the ejector pin seat and the third ejector pin. The third ejector pin assembly is configured in a one-to-one correspondence with the metering chamber.

24. The testing device according to claim 23, characterized in that: The second ejector pin and the third ejector pin are disposed on the same side of the ejector pin seat, and the length of the second ejector pin is greater than the length of the third ejector pin; When the third ejector assembly corresponds to the position of the metering cavity, the second ejector assembly is offset from the position of the overflow cavity.

25. The testing equipment according to claim 22, characterized in that: The testing equipment further includes a transfer mechanism for driving the consumable to move and adjust its position. The transfer mechanism includes an X-axis drive platform for driving the consumable to move along the X-axis direction and a Y-axis drive platform for driving the consumable to move along the Y-axis direction. The X-axis drive platform is disposed on the Y-axis drive platform, and the consumable is disposed on the X-axis drive platform.

26. The testing equipment according to claim 25, characterized in that: The testing equipment also includes a heating monitoring module for monitoring the temperature of samples in the consumables. The heating monitoring module includes a heating element and a temperature sensor. Both the heating element and the temperature sensor are mounted on the X-axis drive platform and located at the bottom of the consumables.

27. The testing equipment according to claim 25, characterized in that: The detection device also includes a detection mechanism, which includes a microscopic imaging component for taking pictures of fluid samples. The microscopic imaging component is movable along a direction that approaches and moves away from the consumable. The detection mechanism also includes a drive mechanism for driving the microscopic imaging component to move. And / or, the detection mechanism further includes a fixedly installed camera and a supplementary light; And / or, the bottom of the consumable is provided with a detection cavity for absorbance detection, and the detection mechanism further includes a light source module for absorbance detection. The detection module includes a light source mounting base, an LED light source mounted on the light source mounting base, a receiver mounting base, and a receiver mounted on the receiver mounting base. The light source mounting base and the receiver mounting base are both mounted on the X-axis drive platform and located on opposite sides of the detection cavity.

28. The testing device according to claim 27, characterized in that: The drive mechanism includes a motor and a reducer connected to the motor. The reducer is a synchronous belt reducer, which includes a housing and a synchronous belt disposed within the housing. The microscopic imaging component is connected to the synchronous belt. The drive mechanism also includes a constant force spring connected between the housing and the microscopic imaging component.

29. A fluid-driven method, characterized in that: The fluid driving method comprises driving the contents of the mixing chamber to mix and flow out of the mixing chamber by a pressing device according to any one of claims 1 to 21, wherein the fluid driving method includes: The sealing mechanism is used to seal the mixing fluid channel, and then the pressing mechanism is used to reciprocate to press the mixing chamber to mix its contents. After the mixing is completed, the sealing mechanism is released from the blockage of the mixing fluid channel, and the pressing mechanism is used to press the mixing chamber to allow the fluid inside to flow out of the mixing chamber.

30. The fluid drive method according to claim 29, characterized in that: The mixing fluid channel is provided with a sealing structure for sealing the mixing fluid channel. The sealing structure can be destroyed by the pressure when the contents of the mixing chamber flow out of the mixing chamber. When the sealing mechanism is used to seal the mixing fluid channel, the pressure head is pressed against the mixing fluid channel and the pressure position is located on the side of the sealing structure away from the mixing chamber. Preferably, there is a gap between the pressure position and the sealing structure; the gap is preferably 0.5 to 1 mm.

31. The fluid drive method according to claim 30, characterized in that: When the pressing component presses the mixing chamber to cause its contents to flow out of the mixing chamber, if the sealing structure is not damaged, the pressing head presses against the mixing fluid channel; if the sealing structure is damaged, the pressing head leaves the mixing fluid channel.

32. A detection method, characterized in that: The detection method comprises performing detection using the detection equipment according to any one of claims 22 to 28, wherein the detection method includes: S1, push the sample into the consumable; S2, after the contents in the mixing chamber are mixed evenly by the fluid driving method according to any one of claims 29 to 31, the fluid in the mixing chamber flows out of the mixing chamber; S3, proceed with the testing.

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