Detection device and detection method for automatically detecting micro-plastics in batches

Abstract

The invention discloses a detection device and a detection method for automatically detecting micro-plastics in batches, and belongs to the technical field of micro-plastic detection. Comprising a microscopic Raman module, an objective table, an automatic sample feeding device, a sample rack and a processor, wherein the microscopic Raman module comprises a microscopic objective lens, a CMOS camera module and a spatial Raman module; a plurality of glass slides are placed on the sample rack; the automatic sample feeding device comprises a sampling hand, a rotating table and a moving assembly; the processor controls the automatic sample feeding device to place a glass slide on a sample rack on an objective table, and the CMOS camera module shoots a sample image of a to-be-detected sample and transmits the sample image to the processor; the spatial Raman module detects the Raman spectrum of the sample to be detected and transmits the Raman spectrum to the processor; the processor compares the Raman spectrum of the to-be-detected sample with an internal database, and the plastic category is obtained through a neural network algorithm; and the processor summarizes the sample image and the Raman spectrum corresponding to each glass slide and the identification and classification result into a detection report.

Description

Technical Field

[0001] This invention relates to the field of microplastic detection equipment technology, and more particularly to an automatic batch detection device and method for microplastics. Background Technology

[0002] Microplastics are plastic particles with a diameter of less than 5 millimeters. They originate from the decomposition of large plastics, the washing of synthetic fibers, and microbeads in personal care products. Microplastics are characterized by high stability, small particle size, and strong mobility, allowing them to persist in the environment for extended periods. They can be transferred through the food chain via animal ingestion and plant accumulation, and can even reach the human body, posing a health risk. Therefore, detecting microplastics in groundwater is of great significance.

[0003] Currently, equipment used for detecting microplastics in groundwater is generally large, requiring laboratory testing. Furthermore, existing testing methods are entirely manual, necessitating manual placement of prepared samples on a micro Raman microscope, manual focusing, and manual particle identification—resulting in extremely low efficiency and inaccurate results. If there are many samples, each one must be tested manually, potentially taking several days to complete, thus requiring significant investment of manpower and time. Summary of the Invention

[0004] One of the main objectives of this invention is to provide an automatic batch detection device for microplastics, in order to solve the problems of existing detection equipment being large in size, inconvenient to carry, requiring manual detection, and having low detection efficiency and inaccuracy.

[0005] The second main objective of this invention is to provide an automatic batch detection device and method for microplastics, so as to solve the problems of low detection efficiency and inaccuracy in the prior art, which require manual detection.

[0006] To solve the above-mentioned technical problems, the present invention is implemented as follows: an automatic batch detection device for microplastics, comprising: A micro Raman module, comprising a microscope objective, a CMOS camera module, and a spatial Raman module; A stage is located below the micro Raman module. A slide placement part is provided on the stage, and a transmission light source is provided below the slide placement part. An adjustment part for adjusting the position of the stage is provided at the bottom of the stage. A sample holder is located on one side of the micro Raman module; multiple glass slides for holding the samples to be tested are placed on the sample holder; An automatic sample feeding device is located on one side of the micro Raman module. The automatic sample feeding device includes a sampling hand, a rotating stage, and a moving component. The rotating stage controls the sampling hand to rotate 360 ​​degrees, and the moving component controls the sampling hand to move horizontally and vertically. The sampling hand places a glass slide on the sample holder onto the stage, or places a glass slide on the stage back onto the sample holder. The processor includes a CMOS camera module, an automatic sample delivery device, a spatial Raman module, and an adjustment unit, all electrically connected to the processor. The processor controls the automatic sample delivery device to place the sample to be tested from the sample holder onto the stage. The CMOS camera module captures an image of the sample to be tested and transmits the captured image to the processor. The spatial Raman module emits a laser beam and focuses it onto the sample to be tested, exciting a Raman spectral signal that is transmitted to the spatial Raman module. The spatial Raman module transmits the Raman spectrum of the sample to the processor. The processor then compiles the sample image and Raman spectrum corresponding to each slide into a test report.

[0007] As a further technical solution, the CMOS camera module includes a dichroic mirror II disposed above the microscope objective, a focusing lens III disposed on one side of the dichroic mirror II, and a CMOS camera disposed on one side of the focusing lens III. The optical center lines of the dichroic mirror II, the focusing lens III, and the CMOS camera coincide, and the optical center line of the microscope objective coincides with that of the dichroic mirror II.

[0008] As a further technical solution, the spatial Raman module includes a dichroic mirror 1 positioned above a dichroic mirror 2, and a bandpass filter, a focusing lens 1, and a laser arranged sequentially on one side of the dichroic mirror 1; the optical center lines of the dichroic mirror 1, the bandpass filter, the focusing lens 1, and the laser coincide; a reflecting mirror is positioned above the dichroic mirror 1, and a long-pass filter, a focusing lens 2, an entrance slit, and a spectrometer are arranged sequentially on one side of the reflecting mirror; the optical center lines of the reflecting mirror, the long-pass filter, the focusing lens 2, the entrance slit, and the spectrometer coincide; the optical center lines of the dichroic mirror 2, the dichroic mirror 1, and the reflecting mirror coincide.

[0009] As a further technical solution, the spectrometer is equipped with a grating, a concave mirror, a collimating mirror, and a CCD.

[0010] As a further technical solution, the slide placement part is an open slot provided on the side of the stage near the automatic sample feeding device. The end of the open slot near the automatic sample feeding device is provided with an opening. Placement stages for supporting the slides are symmetrically provided on both sides of the open slot. The transmission light source is located below the open slot.

[0011] As a further technical solution, two sets of limiting parts are symmetrically provided on both sides of the opening groove. Each limiting part includes a limiting rod, a spring, and a fixing pin. The limiting rod is arranged along the opening direction of the opening groove. The fixing pin is located at the end of the opening groove away from the opening. The end of the limiting rod away from the opening is slidably connected to the fixing pin. The spring is sleeved outside the fixing pin, and the upper and lower ends of the spring abut against the limiting rod and the stage, respectively. The end of the limiting rod away from the fixing pin has an upwardly curved free end. A V-shaped pressing part is provided on the limiting rod near the free end. When the glass slide is placed in the slide placement part, the pressing part abuts against the surface of the glass slide.

[0012] As a further technical solution, the adjustment unit includes a first lead screw slider and a second lead screw slider arranged horizontally, the sliding directions of the first slider on the first lead screw slider and the second slider on the second lead screw slider are perpendicular to each other; the platform is fixed on the first slider of the first lead screw slider, the first lead screw slider is fixed on the second slider of the second lead screw slider, and a fixed base plate is provided below the second lead screw slider.

[0013] As a further technical solution, the sample holder has two vertical and symmetrical vertical plates on both sides, and each vertical plate has multiple platforms arranged in sequence along its vertical direction. The glass slide is placed on the platform opposite to each other between the two vertical plates.

[0014] As a further technical solution, the rotary table includes a turntable and a motor that drives the turntable to rotate 360 ​​degrees; the moving component includes a third lead screw slider and a fourth lead screw slider, the third lead screw slider being horizontally and fixedly mounted on the turntable, and the fourth lead screw slider being vertically and fixedly mounted on the third slider of the third lead screw slider; the sampling hand is fixedly mounted on the fourth slider of the fourth lead screw slider, the sampling hand being horizontally positioned, and the end of the sampling hand away from the fourth lead screw slider is provided with a slot for placing a glass slide.

[0015] An automated batch detection method for microplastics, employing the aforementioned automated batch detection device for microplastics, includes the following steps: S1. Place the glass slides containing the samples to be tested onto the sample holder in sequence and number them. S2. Power on the device and start automatic detection; S3. The processor controls the automatic sample feeding device to place one of the glass slides on the sample holder onto the stage. The processor controls the transmission light source to turn on, and the illumination beam shines on the sample to be tested on the glass slide. The sample particles of the sample to be tested emit scattered light, which is imaged through the microscope objective and then reflected by the dichroic mirror to the focusing lens. The focusing lens focuses the beam and focuses the image onto the CMOS camera. The CMOS camera transmits the acquired sample image to the processor. S4. The processor controls the adjustment unit to automatically move the stage according to the preset displacement parameters, so that the microscope objective can fully scan the slide. The CMOS camera transmits all the sample images captured during the scanning process to the processor, and the processor automatically completes the image stitching to form complete sample image information. S5. The processor identifies the sample particles in the image information, edits the serial number, position coordinates and size of each sample particle, and summarizes the serial number, position coordinates and size of all sample particles on the sample to be tested. S6. The processor controls the laser to turn on, and the laser emits a laser beam. The laser beam is shaped by focusing lens one, and then passes through a bandpass filter to obtain a single wavelength laser beam. It is reflected by dichroic mirror one, and after passing through dichroic mirror two, it enters the microscope objective and illuminates the sample to be tested in the glass slide, forming a spot detection area. S7. The processor moves the sample particles one by one to the light spot detection area for detection based on their position coordinates and through the control and adjustment unit. After the sample particles are excited, they generate Raman light signals. The Raman light signals are coupled into the optical fiber through the microscope objective, dichroic mirror 2, dichroic mirror 1, reflecting mirror, long-pass filter, and focusing lens 2. The Raman light signals enter the entrance slit and reach the spectrometer. After detection by the spectrometer, a Raman spectrum is formed. The processor compares the Raman spectrum of the sample to be tested with the internal database and obtains the plastic category through a neural network algorithm. S8. Once all sample particles on the slide have been collected, the processor automatically summarizes the sample particle information and generates a test report for the slide. S9. The processor controls the automatic sample feeding device to remove the completed slide and put it back in its original position, and then place the next slide on the stage to start the next round of scanning and detection, and so on.

[0016] The beneficial effects of this invention are as follows: 1. In this application, the processor controls all detection steps sequentially and automatically, requiring no real-time human intervention. Steps such as automatic focusing of the microscope objective, automatic image capture and uploading of sample images by the CMOS camera, automatic identification of sample particles by the processor, and automatic detection of the Raman spectra of sample particles are all performed automatically and sequentially. Furthermore, each sample on the slide generates a corresponding test report, which is stored in the processor for later manual verification and traceability. This application also enables automatic batch sample testing, requiring no real-time human intervention. The process is intelligent, convenient, and significantly reduces manual labor, improving testing efficiency while eliminating human error and ensuring high accuracy.

[0017] 2. In this application, the microscopic optical path and Raman optical path of the CMOS camera are integrated coaxially via a dichroic mirror, and both the microscopic and Raman optical paths are arranged laterally, which shortens their vertical height compared to traditional micro-Raman devices, which are much taller overall. Furthermore, the microscopic and Raman optical paths in this application have simple structures, further reducing their overall size. In this embodiment, the micro-Raman module is integrated with the stage, automatic sample feeder, sample holder, and processor, and a housing can be installed externally to form a complete automatic detection device. This device is portable, suitable for laboratory testing, and can also be taken to the field for rapid on-site testing, with a wide range of applications. It also features self-testing upon startup, making operation simple and eliminating the barrier to entry. Attached Figure Description

[0018] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0019] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a three-dimensional structural diagram from another angle in the present invention; Figure 3 This is a schematic diagram of the optical path inside the micro Raman module of the present invention; Figure 4 for Figure 1 A magnified view of part A in the image.

[0020] Explanation of reference numerals in the attached figures 1. Spatial Raman Module; 10. Dichroic Mirror I; 11. Bandpass Filter; 12. Focusing Lens I; 13. Laser; 14. Mirror; 15. Long-pass Filter; 16. Focusing Lens II; 17. Entrance Slit; 18. Spectrometer; 180. Grating; 181. Concave Mirror; 182. Collimating Lens; 183. CCD; 19. Fifth Screw Slider; 190. Fifth Slider; 2. Microscope Objective; 20. Processor; 3. CMOS Camera Module; 30. CMOS Camera; 31. Focusing Lens III; 32. Dichroic Mirror II; 4. Stage; 40. Opening Slot; 41. Placement Stage; 410. Curvature; 5. Limiting part; 50. Limiting rod; 51. Spring; 52. Fixing pin; 53. Free end; 54. Pressing part; 6. Adjusting part; 60. First lead screw slider; 61. Second lead screw slider; 62. Fixed base plate; 63. First slider; 64. Second slider; 7. Transmitted light source; 8. Sample holder; 80. Vertical plate; 81. Stage; 82. Glass slide; 9. Automatic sample feeding device; 90. Rotary table; 900. Turntable; 901. Motor; 91. Third lead screw slider; 92. Third slider; 93. Fourth lead screw slider; 94. Fourth slider; 95. Sampling hand; 96. Slot. Detailed Implementation

[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below. Example

[0022] like Figures 1-4 As shown, this invention proposes an automated batch detection device for microplastics, comprising: a micro Raman module and a stage 4 disposed below the micro Raman module, wherein the stage 4 is provided with a slide placement part for placing a glass slide 82 carrying the sample to be tested. Below the slide placement part is a transmission light source 7 that can provide an illumination beam. The micro Raman module further includes a microscope objective 2 for scanning the sample to be tested, a CMOS camera module 3 for capturing images of the sample, and a spatial Raman module 1; the spatial Raman module 1 emits a laser beam and focuses the laser beam onto the sample to be tested, exciting a Raman spectral signal from the sample to be tested, which is then transmitted to the spatial Raman module 1.

[0023] Among them, such as Figure 3 As shown, the CMOS camera module 3 includes a dichroic mirror 32 positioned above the microscope objective 2, a focusing lens 31 positioned to one side of the dichroic mirror 32, and a CMOS camera 30 positioned on the side of the focusing lens 31 away from the dichroic mirror 32. The dichroic mirror 32 is tilted at 45 degrees, and the optical center lines of the dichroic mirror 32, the focusing lens 31, and the CMOS camera 30 coincide. The optical center line of the microscope objective 2 coincides with that of the dichroic mirror 32. Figure 3As shown, the spatial Raman module 1 includes a dichroic mirror 10 positioned above a dichroic mirror 32, and a bandpass filter 11, a focusing lens 12, and a laser 13 arranged sequentially on one side of the dichroic mirror 10. The dichroic mirror 10 is tilted at 45 degrees, the focusing lens 12 is positioned on the side of the bandpass filter 11 away from the dichroic mirror 10, and the laser 13 is positioned on the side of the focusing lens 12 away from the bandpass filter 11. The optical center lines of the dichroic mirror 10, bandpass filter 11, focusing lens 12, and laser 13 coincide. A reflecting mirror 14 is positioned above the dichroic mirror 10, and a long-pass filter 15, a focusing lens 16, an entrance slit 17, and a spectrometer 18 are arranged sequentially on one side of the reflecting mirror 14. The reflector 14 is tilted at 45 degrees. The second focusing lens 16 is positioned on the side of the long-pass filter 15 away from the reflector 14. The entrance slit 17 is positioned on the side of the second focusing lens 16 away from the long-pass filter 15. The spectrometer 18 is positioned on the side of the entrance slit 17 away from the second focusing lens 16. The optical center lines of the reflector 14, the long-pass filter 15, the second focusing lens 16, the entrance slit 17, and the spectrometer 18 coincide. The optical center lines of the second dichroic mirror 32, the first dichroic mirror 10, and the reflector also coincide. In this embodiment, a laser beam is generated by laser 13, which emits a 785nm laser beam. The laser beam passes sequentially through focusing lens 12 and bandpass filter 11. The laser beam undergoes spot shaping when passing through focusing lens 12, and then passes through bandpass filter 11 to obtain a single-wavelength laser beam. It then passes through dichroic mirror 10 and is reflected by dichroic mirror 10 to dichroic mirror 32 before entering microscope objective 2. After being focused by microscope objective 2, it illuminates the sample under test (i.e., the glass slide 82 carrying the sample). The sample under test is excited to generate a Raman light signal. The Raman light signal sequentially passes through microscope objective 2, dichroic mirror 32, dichroic mirror 10, reflecting mirror 14, long-pass filter 15, and focusing lens 16 before coupling into an optical fiber. The Raman light signal then enters entrance slit 17 and reaches spectrometer 18. Figure 3 As shown, the spectrometer 18 contains a grating 180, a concave mirror 181, a collimating mirror 182, and a CCD 183. The Raman light signal enters the entrance slit 17, passes through the collimating mirror 182, and then illuminates the grating 180. After being dispersed by the grating 180, the light is focused by the concave mirror 181 onto the CCD (charge-coupled device) 183. The CCD 183 detects the dispersed information to obtain the Raman spectrum.

[0024] In this embodiment, after the laser beam illuminates the surface of the sample, the sample undergoes reflection, Rayleigh scattering, and Raman scattering. Then, when passing through the long-pass filter 15, the long-pass filter 15 filters out the reflected and Rayleigh scattered light signals, allowing the Raman scattered light signal to pass through. Furthermore, the spectrometer 18 in this application is a cooled Raman spectrometer 18. The Raman scattered light enters the cooled Raman spectrometer 18 after passing through the entrance slit 17, thereby obtaining the Raman spectrum of the sample. Additionally, the dichroic mirror 32 in this embodiment integrates the microscopic optical path and the Raman optical path of the CMOS camera 30 in the system into a coaxial optical path, selectively allowing the Raman band to pass through and reflecting the illumination beam generated by the transmitted light source 7.

[0025] In addition, the transmission light source 7 in this embodiment is selected as a transmission Kohler illumination with a visible light wavelength of 400-700nm. The illumination beam shines on the sample to be tested, which may have multiple sample particles. When the illumination beam shines on the sample particles, the sample particles emit scattered light, which is imaged by the objective lens. Then, when it passes through the dichroic mirror 32 placed at 45 degrees, it is reflected and focused by the focusing lens 31 onto the photosensitive chip of the CMOS camera 30. The CMOS camera 30 acquires information and presents an image of the sample particles. The image of the sample particles can reflect the morphology and size of the sample particles.

[0026] In this embodiment, the microscopic optical path and Raman optical path of the CMOS camera 30 are coaxially integrated through a dichroic mirror 32, and both the microscopic and Raman optical paths are arranged laterally, which shortens their vertical height. Compared with traditional micro-Raman equipment, its height is greatly reduced. In addition, the structure of the microscopic and Raman optical paths in this application is simple, thereby reducing its overall size. Furthermore, in this embodiment, the micro-Raman module is integrated with the stage 4, automatic sample feeding device 9, sample holder 8, processor 20, etc., and a housing (not shown in the figure) can be installed on its exterior to form a complete automatic detection device. This device is portable and can be used for laboratory testing or on-site testing.

[0027] like Figure 1 As shown, this embodiment also includes a sample holder 8, which is located on one side of the micro Raman module; the sample holder 8 holds multiple glass slides 82 for carrying the samples to be tested. Two vertical plates 80 are vertically and symmetrically arranged on both sides of the sample holder 8, and multiple stages 81 are arranged sequentially along the vertical direction on each vertical plate 80, and the glass slides 82 are placed on the opposing stages 81 between the two vertical plates 80.

[0028] like Figure 1 and Figure 2As shown in the figure, this embodiment further includes an automatic sample feeding device 9, which is located on one side of the micro-Raman module. The automatic sample feeding device 9 includes a sampling hand 95, a rotating table 90 and a moving component. The rotating table 90 controls the sampling hand 95 to rotate 360 degrees, and the moving component controls the sampling hand 95 to move horizontally and vertically; the sampling hand 95 places the glass slide 82 on the sample rack 8 onto the stage 4, or returns the glass slide 82 on the stage 4 to the sample rack 8. The rotating table 90 includes a turntable 900 and a motor 901 that drives the turntable 900 to rotate 360 degrees (the structure of the motor 901 driving the turntable 900 to rotate is prior art and will not be elaborated here). The moving component includes a third screw slider 91 and a fourth screw slider 93. The third screw slider 91 is horizontally and fixedly arranged on the turntable 900, and the fourth screw slider 93 is vertically and fixedly arranged on the third slider 92 of the third screw slider 91. The sampling hand 95 is fixedly arranged on the fourth slider 94 of the fourth screw slider 93. The sampling hand 95 is horizontally arranged, and a card slot 96 for placing the glass slide 82 is provided at one end of the sampling hand 95 away from the fourth screw slider 93. In this embodiment, the rotating table 90 and the moving component drive the sampling hand 95 to move to the lower side of the corresponding glass slide 82 on the sample rack 8, and make the glass slide 82 correspondingly located at the card slot 96 of the sampling hand 95. Then the sampling hand 95 moves upward to place the glass slide 82 into the card slot 96. After that, the sampling hand 95 removes the glass slide 82 from the sample rack 8 and sends it to the stage 4.

[0029] As Figure 1 , Figure 2 and Figure 4 shown in the figure, the glass slide placement part on the stage 4 in this application is an opening groove 40 provided on the side of the stage 4 close to the automatic sample feeding device 9. An opening is provided at one end of the opening groove 40 close to the automatic sample feeding device 9. Placement platforms 41 for carrying the glass slide 82 are symmetrically provided on both sides of the opening groove 40, and the transmission light source 7 is located below the opening groove 40. In this embodiment, the opening groove 40 is arranged in a "匚" shape, and the opening of the "匚" shape faces the automatic sample feeding device 9. In this embodiment, the transmission light source 7 and the stage 4 are of a split structure. In addition, limiting parts 5 for pressing the glass slide 82 are symmetrically provided on both sides of the opening groove 40. The limiting parts 5 include limiting rods 50, springs 51 and fixing pins 52. The limiting rods 50 are arranged along the opening direction of the opening groove 40. The fixing pins 52 are arranged at one end of the opening groove 40 away from the opening. The end of the limiting rod 50 away from the opening is slidably connected to the fixing pin 52, that is, the fixing pin 52 is inserted through the corresponding end of the limiting rod 50. The spring 51 is sleeved outside the fixing pin 52, and the upper end of the spring 51 abuts against the limiting rod 50, and the lower end abuts against the stage 4. A free end 53 that翘向上 is provided at one end of the limiting rod 50 away from the fixing pin 52. A downwardly bent V-shaped pressing part 54 is provided at a position on the limiting rod 50 close to the free end 53. When the glass slide 82 is placed on the glass slide placement part, the pressing part 54 abuts against the surface of the glass slide 82. As Figure 1 and Figure 4 As shown, in this embodiment, the placement stage 41 has an outwardly expanding arc 410 at one end near the opening of the slot 40, which facilitates the insertion of the glass slide 82 into the placement stage 41 (the two sides of the glass slide 82 are respectively located on the placement stage 41). In this embodiment, the width of the sampling hand 95 is smaller than the width of the opening slot 40. When placing the slide 82, the sampling hand 95 first extends into the opening slot 40 and gradually pushes the slide 82 from the opening of the opening slot 40 inward until the entire slide 82 is pushed onto the placement stage 41. The free end 53 of the limiting rod 50 is tilted upward, which can widen the distance between the limiting rod 50 and the placement stage 41, thereby facilitating the pushing of the slide 82. After the slide 82 is placed into the opening slot 40, under the elastic force of the spring 51, the pressing part 54 presses against the surface of the slide 82, thereby limiting the slide 82. The sampling hand 95 first moves downward, away from the slide 82, and then moves away from the stage 4 to leave the stage 4.

[0030] Other examples Figure 1 and Figure 2 As shown, the bottom of the platform 4 is provided with an adjustment part 6 for adjusting the position of the platform 4; the adjustment part 6 includes a first lead screw slider 60 and a second lead screw slider 61 arranged horizontally, the sliding directions of the first slider 63 on the first lead screw slider 60 and the second slider 64 on the second lead screw slider 61 are perpendicular to each other; the platform 4 is fixed on the first slider 63 of the first lead screw slider 60, the first lead screw slider 60 is fixed on the second slider 64 of the second lead screw slider 61, and a fixed base plate 62 is provided below the second lead screw slider.

[0031] like Figure 1 and Figure 2 As shown, in this embodiment, a fifth lead screw slider 19 is provided on one side of the micro Raman module, which drives it to move closer to or further away from the stage 4. The fifth lead screw slider 19 is fixedly set, and the micro Raman module is fixed on the fifth slider 190 of the fifth lead screw slider 19. The fifth lead screw slider 19 can adjust the position of the micro Raman module, thereby adjusting the distance between the microscope objective 2 and the glass slide 82, and performing automatic focusing according to the actual situation.

[0032] like Figure 3As shown, this embodiment also includes a processor 20, wherein the CMOS camera module 3, the automatic sample delivery device 9, the spatial Raman module 1, the adjustment part 6 of the stage 4, and the fifth lead screw slider 19 are all electrically connected to the processor 20. The processor 20 controls the automatic sample delivery device 9 to place the glass slide 82 on the sample holder 8 onto the stage 4 for detection. The processor 20 also controls the fifth lead screw slider 19 to move the micro Raman module, thereby achieving automatic focusing. The CMOS camera module 3 is responsible for transmitting the sample images captured on each glass slide 82 to the processor 20; the spatial Raman module 1 transmits the Raman spectra of the samples on each glass slide 82 to the processor 20; the processor 20 compares the Raman spectra of the samples to be tested with its internal database and obtains the plastic category through a neural network algorithm; the processor 20 summarizes the sample images, Raman spectra, and plastic category information corresponding to each glass slide 82 into a test report.

[0033] The microplastic detection device in this embodiment can be applied to detect microplastics in other fields such as groundwater, soil, or atmosphere. Example

[0034] like Figures 1-4 As shown, an automatic batch detection device and method for detecting microplastics, using the aforementioned automatic batch detection device for microplastics, includes the following steps: S1. Manually place the prepared glass slides 82 carrying the samples to be tested onto the sample holder 8 in sequence and number them (the numbering can be based on the number of layers in the sample holder, and each layer of glass slides corresponds to the number of layers in that layer of the sample holder. This number is pre-entered into the processor).

[0035] S2. Power on the device and enable automatic detection mode; S3. The processor 20 controls the automatic sample feeding device 9 to place one of the glass slides 82 on the sample holder 8 onto the stage 4; the processor 20 controls the transmission light source 7 to turn on, and the illumination beam shines on the sample to be tested on the glass slide 82 (wherein the processor 20 can adjust the fifth lead screw module 19 to make the microscope objective 2 automatically focus); the sample particles of the sample to be tested emit scattered light, the scattered light is imaged by the microscope objective 2, and then reflected by the dichroic mirror 2 32 to the focusing lens 3 31. The focusing lens 3 31 focuses the beam and focuses the image onto the CMOS camera 30. The CMOS camera 30 transmits the acquired sample image to the processor 20.

[0036] S4. The processor 20 controls the adjustment unit 6 to automatically move the stage 4 according to the preset displacement parameters, so that the microscope objective 2 can fully scan the slide 82. The CMOS camera 30 transmits all the sample images captured during the scanning process to the processor 20. The processor 20 automatically completes the image stitching to form complete sample image information. The displacement parameters can be preset according to the actual size of the slide 82, so that the microscope objective 2 can fully scan the slide 82.

[0037] S5, the processor 20 identifies the sample particles in the image information, edits the serial number, position coordinates and size of each sample particle, and summarizes the serial number, position coordinates and size of all sample particles on the sample to be tested.

[0038] S6. The processor 20 controls the laser 13 to turn on, and the laser 13 emits a laser beam. The laser beam is shaped by the focusing lens 12, and a single wavelength laser beam is obtained by passing through the bandpass filter 11. It is reflected by the dichroic mirror 10, and after passing through the dichroic mirror 32, it enters the microscope objective 2 and irradiates the glass slide 82 to form a spot detection area.

[0039] S7. The processor 20 moves the sample particles one by one to the light spot detection area for detection based on their position coordinates and through the control and adjustment unit 6. After being excited in the light spot detection area, the sample particles generate Raman light signals. These Raman light signals are coupled into the optical fiber through the microscope objective 2, dichroic mirror 32, dichroic mirror 10, mirror 14, long-pass filter 15, and focusing lens 16. The Raman light signals then enter the entrance slit 17 and reach the spectrometer 18, where they are detected to form a Raman spectrum. After entering the entrance slit 17, the Raman light signals pass through the collimating lens 182 and illuminate the grating 180. The grating 180 disperses the light, which is then converged by the concave mirror 181 onto the CCD 183. The CCD 183 detects the dispersed information to obtain the Raman spectrum. The processor 20 compares the Raman spectrum of the sample with its internal database and uses a neural network algorithm to determine the plastic category. S8. Based on the serial number of all sample particles on the slide 82, all sample particles are detected sequentially. The processor 20 automatically summarizes the information of all sample particles on the slide 82 and forms a test report for the slide 82.

[0040] S9, the processor 20 controls the automatic sample feeding device 9 to remove the completed slide 82 and return it to its original position, and place the next slide 82 on the stage 4 to start the next round of scanning and detection, and so on. The detection of the slides 82 on the sample holder 8 can be carried out sequentially from top to bottom or from bottom to top, or in any other order.

[0041] In this embodiment, the processor 20 controls all detection steps sequentially and automatically, without human intervention. Steps such as automatic focusing, automatic image capture and uploading, automatic sample particle identification, and automatic detection of the Raman spectra of sample particles are all performed automatically and in sequence. Each sample on each slide 82 generates a corresponding test report, which is stored in the processor 20 for later manual review. Each slide 82 on the sample holder 8 is numbered, and each test report corresponds to a specific slide number, ensuring clarity and traceability of the test reports.

[0042] Furthermore, all the lead screws and sliders in Embodiments 1 and 2 are existing technologies, and their working principles will not be elaborated further. In addition, the processor 20 sequentially controls the automatic sample feeding device 9, the adjustment unit 6, the fifth lead screw and slider 19, the micro Raman module, etc., through a computer program. The processor also automatically performs image stitching, automatically identifies sample particles, and performs sample particle numbering, position coordinate labeling, and size measurement, all of which are performed using a computer control program, and this control program is all existing technology.

[0043] The above description is merely a preferred embodiment of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

Claims

1. An automatic batch detection device for microplastics, characterized in that, include: A micro Raman module, comprising a microscope objective, a CMOS camera module, and a spatial Raman module; A stage is located below the micro Raman module. A slide placement part is provided on the stage, and a transmission light source is provided below the slide placement part. An adjustment part for adjusting the position of the stage is provided at the bottom of the stage. A sample holder is located on one side of the micro Raman module; multiple glass slides for holding the samples to be tested are placed on the sample holder; An automatic sample feeding device is located on one side of the micro Raman module. The automatic sample feeding device includes a sampling hand, a rotating stage, and a moving component. The rotating stage controls the sampling hand to rotate 360 ​​degrees, and the moving component controls the sampling hand to move horizontally and vertically. The sampling hand places a glass slide on the sample holder onto the stage, or places a glass slide on the stage back onto the sample holder. The processor includes a CMOS camera module, an automatic sample delivery device, a spatial Raman module, and an adjustment unit, all electrically connected to the processor. The processor controls the automatic sample delivery device to place the sample to be tested from the sample holder onto the stage. The CMOS camera module captures an image of the sample to be tested and transmits the captured image to the processor. The spatial Raman module emits a laser beam and focuses it onto the sample to be tested, exciting a Raman spectral signal that is transmitted to the spatial Raman module. The spatial Raman module transmits the Raman spectrum of the sample to the processor. The processor then compiles the sample image and Raman spectrum corresponding to each slide into a test report.

2. The automatic batch detection device for microplastics according to claim 1, characterized in that, The CMOS camera module includes a dichroic mirror II disposed above the microscope objective, a focusing lens III disposed on one side of the dichroic mirror II, and a CMOS camera disposed on one side of the focusing lens III. The optical center lines of the dichroic mirror II, the focusing lens III, and the CMOS camera coincide, and the optical center line of the microscope objective coincides with that of the dichroic mirror II.

3. The automatic batch detection device for microplastics according to claim 2, characterized in that, The spatial Raman module includes a dichroic mirror 1 positioned above a dichroic mirror 2, and a bandpass filter, a focusing lens 1, and a laser arranged sequentially on one side of the dichroic mirror 1. The optical center lines of the dichroic mirror 1, the bandpass filter, the focusing lens 1, and the laser coincide. A reflector is positioned above the dichroic mirror 1, and a long-pass filter, a focusing lens 2, an entrance slit, and a spectrometer are arranged sequentially on one side of the reflector. The optical center lines of the reflector, the long-pass filter, the focusing lens 2, the entrance slit, and the spectrometer coincide. The optical center lines of the dichroic mirror 2, the dichroic mirror 1, and the reflector coincide.

4. The automatic batch detection device for microplastics according to claim 3, characterized in that, The spectrometer contains a grating, a concave mirror, a collimating mirror, and a CCD.

5. The automatic batch detection device for microplastics according to claim 1, characterized in that, The slide placement part is an open slot provided on the side of the stage near the automatic sample feeding device. The end of the open slot near the automatic sample feeding device has an opening. Placement stages for supporting glass slides are symmetrically provided on both sides of the open slot. The transmission light source is located below the open slot.

6. The automatic batch detection device for microplastics according to claim 5, characterized in that, Two sets of limiting parts are symmetrically arranged on both sides of the opening groove. Each limiting part includes a limiting rod, a spring, and a fixing pin. The limiting rod is arranged along the opening direction of the opening groove. The fixing pin is located at the end of the opening groove away from the opening. The end of the limiting rod away from the opening is slidably connected to the fixing pin. The spring is sleeved on the outside of the fixing pin, and the upper and lower ends of the spring abut against the limiting rod and the stage, respectively. The end of the limiting rod away from the fixing pin has an upward-curving free end. Near the free end of the limiting rod, there is a V-shaped pressing part. When the slide is placed in the slide placement part, the pressing part abuts against the surface of the slide.

7. The automatic batch detection device for microplastics according to claim 1, characterized in that, The adjustment unit includes a first lead screw slider and a second lead screw slider arranged horizontally, and the sliding directions of the first slider on the first lead screw slider and the second slider on the second lead screw slider are perpendicular to each other; the platform is fixed on the first slider of the first lead screw slider, the first lead screw slider is fixed on the second slider of the second lead screw slider, and a fixed base plate is provided below the second lead screw slider.

8. The automatic batch detection device for microplastics according to claim 1, characterized in that, The sample holder has two vertical and symmetrical vertical plates on both sides. Each vertical plate has multiple platforms arranged in sequence along its vertical direction. The glass slide is placed on the platform opposite to the vertical plate.

9. The automatic batch detection device for microplastics according to claim 1, characterized in that, The rotating stage includes a turntable and a motor that drives the turntable to rotate 360 ​​degrees; the moving component includes a third lead screw slider and a fourth lead screw slider, the third lead screw slider being horizontally and fixedly mounted on the turntable, and the fourth lead screw slider being vertically and fixedly mounted on the third slider of the third lead screw slider; the sampling hand is fixedly mounted on the fourth slider of the fourth lead screw slider, the sampling hand being horizontally positioned, and the end of the sampling hand away from the fourth lead screw slider having a slot for placing a glass slide.

10. A method for automatically batch detecting microplastics, employing the automatic batch detecting microplastics detection device according to any one of claims 1-9, characterized in that, Includes the following steps: S1. Place the glass slides containing the samples to be tested onto the sample holder in sequence and number them. S2. Power on the device and start automatic detection; S3. The processor controls the automatic sample feeding device to place one of the glass slides on the sample holder onto the stage. The processor controls the transmission light source to turn on, and the illumination beam shines on the sample to be tested on the glass slide. The sample particles of the sample to be tested emit scattered light, which is imaged through the microscope objective and then reflected by the dichroic mirror to the focusing lens. The focusing lens focuses the beam and focuses the image onto the CMOS camera. The CMOS camera transmits the acquired sample image to the processor. S4. The processor controls the adjustment unit to automatically move the stage according to the preset displacement parameters, so that the microscope objective can fully scan the slide. The CMOS camera transmits all the sample images captured during the scanning process to the processor, and the processor automatically completes the image stitching to form complete sample image information. S5. The processor identifies the sample particles in the image information, edits the serial number, position coordinates and size of each sample particle, and summarizes the serial number, position coordinates and size of all sample particles on the sample to be tested. S6. The processor controls the laser to turn on, and the laser emits a laser beam. The laser beam is shaped by focusing lens one, and then passes through a bandpass filter to obtain a single wavelength laser beam. It is reflected by dichroic mirror one, and after passing through dichroic mirror two, it enters the microscope objective and illuminates the sample to be tested in the glass slide, forming a spot detection area. S7. The processor moves the sample particles one by one to the light spot detection area for detection based on their position coordinates and through the control and adjustment unit. After the sample particles are excited, they generate Raman light signals. The Raman light signals are coupled into the optical fiber through the microscope objective, dichroic mirror 2, dichroic mirror 1, reflecting mirror, long-pass filter, and focusing lens 2. The Raman light signals enter the entrance slit and reach the spectrometer. After detection by the spectrometer, a Raman spectrum is formed. The processor compares the Raman spectrum of the sample to be tested with the internal database and obtains the plastic category through a neural network algorithm. S8. Once all sample particles on the slide have been collected, the processor automatically summarizes the sample particle information and generates a test report for the slide. S9. The processor controls the automatic sample feeding device to remove the completed slide and put it back in its original position, and then place the next slide on the stage to start the next round of scanning and detection, and so on.

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