An automated imaging apparatus for circulating tumor cells

Abstract

The application discloses an automatic imaging equipment for circulating tumor cells, and belongs to the technical field of cell imaging. In order to solve the problem that an electric Z-axis module is prone to mechanical wear under long-term use and affects positioning accuracy, the application comprises a shell, a camera is arranged in the shell, an electric filter rotating wheel is arranged in the shell, the camera is connected with the electric filter rotating wheel, a fluorescent imaging light path is arranged in the shell, the fluorescent imaging light path is connected with the electric filter rotating wheel, and a fluorescent illumination light path is fixedly connected to the top of the fluorescent imaging light path. The inner wall of the first threaded block is convenient to clean, the jamming phenomenon is avoided, the abrasion of the two first threaded plates caused by more blockings is avoided, the two second threaded plates are convenient to directly replace the two first threaded plates for use, and the positioning accuracy of the Z-axis direction is not affected due to the excessive abrasion of the two first threaded plates.

Description

Technical Field

[0001] This invention relates to the field of cell imaging technology, specifically to an automated imaging device for circulating tumor cells. Background Technology

[0002] Circulating tumor cells (CTCs) refer to tumor cells that detach from the primary tumor and enter the peripheral blood circulation. Automated imaging equipment for CTCs is a key tool for CTC detection. Such equipment typically integrates a fluorescence imaging optical path, a displacement stage, a motorized Z-axis module, a motorized filter wheel, and an automatic endoscope switching module. Through the coordinated operation of these components, automated identification and analysis of CTCs are achieved, providing crucial support for clinical diagnosis and treatment monitoring. The motorized Z-axis control module is used to achieve high-precision focal plane adjustment and, in conjunction with image clarity assessment, achieves automatic focusing. Its positioning accuracy directly affects image clarity and cell identification accuracy.

[0003] In current automated imaging equipment for circulating tumor cells, the electric Z-axis module is prone to mechanical wear over long-term use, resulting in decreased positioning accuracy. At the same time, dust particles in the operating environment can easily penetrate into the electric Z-axis module, mixing with the lubricant to form clumps. When there are many clumps, it can cause jamming of the electric Z-axis module, further aggravating component wear and further affecting positioning accuracy, thus impacting the stability and reliability of circulating tumor cell detection.

[0004] To address the above issues, an automated imaging device for circulating tumor cells is proposed. Summary of the Invention

[0005] The purpose of this invention is to provide an automated imaging device for circulating tumor cells. By using this device, the problems mentioned above are solved, such as the mechanical wear of the electric Z-axis module under long-term use, and the fact that dust can easily clump together with the lubricant in the electric Z-axis module, thus affecting the positioning accuracy.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] An automated imaging device for circulating tumor cells includes a housing, within which a camera is mounted. An electrically driven filter wheel is also mounted within the housing and connected to the filter wheel. A fluorescence imaging optical path is mounted within the housing and connected to the filter wheel. A fluorescence illumination optical path is fixedly connected to the top of the fluorescence imaging optical path. A first support frame is provided within the housing, to which both the fluorescence imaging and illumination optical paths are fixedly connected. A second support frame is provided within the housing, with a first L-shaped support plate fixedly connected to its top. An LED light is mounted within the first L-shaped support plate. A rotating assembly is provided within the housing, with two first threaded plates and two... A second threaded plate, a lifting component is provided on one side of the rotating component, the lifting component is threadedly connected to two first threaded plates and two second threaded plates respectively, fiber optic sensors and laser contour sensors are installed on both sides of the lifting component, a drive component is fixedly connected to one side of the rotating component, an adjustment component is slidably connected inside the rotating component, the drive component and the adjustment component are rotatably connected, a snap-fit ​​component is provided inside the rotating component, the snap-fit ​​component is connected to the adjustment component, a cleaning component is slidably connected inside the rotating component, a collection component is provided on one side of the lifting component, a first moving component is fixedly connected to the top of the lifting component, a second moving component is fixedly connected to the top of the first moving component, and a sample holder is fixedly connected inside the second moving component.

[0008] Furthermore, the rotating assembly includes a third support plate fixedly connected to the housing, a first servo motor installed inside the third support plate, a rotating shaft fixedly connected to the output end of the first servo motor, a connecting block fixedly connected to one side of the third support plate, the rotating shaft being rotatably connected to the connecting block, and two first threaded plates and two second threaded plates being slidably connected to the rotating shaft.

[0009] Furthermore, the lifting assembly includes a first threaded block that is threadedly connected to two first threaded plates and two second threaded plates respectively, two limiting blocks that are fixedly connected to one side of the third support plate, slide grooves that are fixedly connected to both sides of the first threaded block, a slide rod that is fixedly connected to one side of the third support plate, the slide grooves and the slide rods being slidably connected, and a lifting frame that is fixedly connected to the top of the two slide grooves.

[0010] Furthermore, the drive assembly includes a second L-shaped support plate fixedly connected to one side of the third support plate, and an electric push rod is installed on one side of the second L-shaped support plate.

[0011] Furthermore, the adjustment assembly includes a rotating block rotatably connected to the movable end of the electric push rod. A lifting rod is fixedly connected to the bottom of the rotating block. The lifting rod is slidably connected to the rotating shaft. Several first inclined blocks are connected to each other on the outer ring of the lifting rod. Several second inclined blocks are fixedly connected to each side of the two second threaded plates. The first inclined blocks and the second inclined blocks are in contact. Several L-shaped inclined blocks are connected to each other on the outer ring of the lifting rod. Inclined grooves are fixedly connected to each of the two first threaded plates. The L-shaped inclined blocks are in contact with the inclined grooves. A T-shaped moving rod is fixedly connected to each side of the first inclined block and the L-shaped inclined block. A T-shaped moving groove is opened on each side of the second inclined block and the inclined groove. The T-shaped moving rod is slidably connected to the T-shaped moving groove. Rollers are rotatably connected to both sides of the T-shaped moving rod. The rollers are in rolling contact with the T-shaped moving groove.

[0012] Furthermore, the snap-fit ​​assembly includes two slide plates slidably connected within the rotating shaft. Two first springs are fixedly connected to one side of each slide plate, and the other ends of the two first springs are fixedly connected to the inner wall of the rotating shaft. A snap-fit ​​block is fixedly connected to one side of each slide plate. Two first snap-fit ​​slots and a second snap-fit ​​slot are respectively opened on the outer ring of the lifting rod. A first electromagnet is installed on the inner wall of the rotating shaft, and a first magnet block is fixedly connected to one side of each slide plate.

[0013] Furthermore, the cleaning assembly includes two limiting rods fixedly connected inside the rotating shaft. Each limiting rod has a movable block slidably connected to its outer wall. The movable block is slidably connected to the inside of the rotating shaft. A second spring is fixedly connected to the top of the movable block, and the other end of the second spring is fixedly connected to the inner wall of the rotating shaft. Two second electromagnets are installed inside the rotating shaft. A second magnet is fixedly connected inside the movable block. A third spring is fixedly connected to one side of the movable block. A cleaning block is slidably connected to one side of the movable block. A third electromagnet is installed on one side of the movable block. A third magnet is fixedly connected to one side of the cleaning block.

[0014] Furthermore, the collection component includes a mounting plate fixedly connected to one side of the limiting block, and a collection frame fixedly connected to one side of each of the two mounting plates, with the two collection frames fitting together.

[0015] Furthermore, the first moving component includes a first adjusting frame fixedly connected to the top of the lifting frame, a second servo motor mounted on one side of the first adjusting frame, a first threaded rod fixedly connected to the output end of the second servo motor, the first threaded rod being rotatably connected to the first adjusting frame, a second threaded block being threadedly connected to the outer wall of the first threaded rod, the second threaded block being slidably connected to the first adjusting frame, a second adjusting frame fixedly connected to the top of the second threaded block, and a first positioning block fixedly connected to the bottom of the second adjusting frame, the first positioning block being slidably connected to the first adjusting frame.

[0016] Furthermore, the second moving component includes a third servo motor mounted on one side of the second adjusting frame. The output end of the third servo motor is fixedly connected to a second threaded rod, which is rotatably connected to the second adjusting frame. A third threaded block is threadedly connected to the outer wall of the second threaded rod, which is slidably connected to the second adjusting frame. The top of the third threaded block is fixedly connected to the third adjusting frame, and the bottom of the third adjusting frame is fixedly connected to a second positioning block, which is slidably connected to the second adjusting frame. A sample holder is fixedly connected inside the third adjusting frame.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0018] When the fiber optic sensor determines that the clumps formed on the outer walls of the two first threaded plates exceed the normal range, the rotating component, in conjunction with the cleaning component, rotates and descends within the lifting component, thereby facilitating the cleaning of the inner wall of the lifting component, avoiding jamming, and preventing excessive clumps from causing accelerated wear on the two first threaded plates.

[0019] When the laser profile sensor detects severe wear on the surfaces of the two first threaded plates, it facilitates the direct replacement of the two first threaded plates with two second threaded plates. This allows the two second threaded plates to connect with the lifting assembly, thereby preventing excessive wear of the two first threaded plates from affecting the positioning accuracy in the Z-axis direction. This ensures the stability and data accuracy of multi-channel fluorescence imaging, while also shortening equipment downtime for maintenance, ensuring the continuity of circulating tumor cell imaging detection, and reducing the cost of consumable replacements and the complexity of maintenance.

[0020] During use, the fiber optic sensor can also determine whether the clumps formed on the outer wall of the two second threaded plates exceed the normal range by changing the reflection of light signals. If they exceed the normal range, the inner wall of the lifting component can be cleaned by the cleaning component, thereby avoiding excessive clumps that could lead to increased wear on the two second threaded plates. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall internal structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the overall structure of the present invention;

[0023] Figure 3 This is a schematic diagram of the overall internal side view structure of the present invention;

[0024] Figure 4 for Figure 3 Enlarged view of point A;

[0025] Figure 5 This is a schematic diagram showing the connection relationship between the rotating component, lifting component, fiber optic sensor, and adjusting component of the present invention.

[0026] Figure 6 This is a schematic diagram showing the connection relationship between the rotating component, lifting component, driving component, and adjusting component of the present invention.

[0027] Figure 7 for Figure 6 Enlarged view of point B;

[0028] Figure 8 This is a top view schematic diagram showing the connection relationship between the rotating assembly, the first threaded plate, the second threaded plate, the lifting assembly, and the fiber optic sensor of the present invention.

[0029] Figure 9 This is a schematic diagram showing the connection relationship between the rotating assembly, the first threaded plate, the second threaded plate, and the adjusting assembly of the present invention.

[0030] Figure 10 for Figure 9 Enlarged view of point C;

[0031] Figure 11 This is a cross-sectional structural diagram showing the connection relationship between the rotating component, the first threaded plate, and the adjusting component of the present invention.

[0032] Figure 12 for Figure 11 Enlarged view of point D;

[0033] Figure 13 This is a cross-sectional structural diagram showing the connection relationship between the rotating assembly, the second threaded plate, and the adjusting assembly of the present invention.

[0034] Figure 14 for Figure 13 Enlarged view of point E;

[0035] Figure 15 This is a cross-sectional structural diagram showing the connection relationship between the rotating assembly, the second threaded plate, the adjusting assembly, and the cleaning assembly of the present invention.

[0036] Figure 16 This is a schematic cross-sectional view of the adjustment component of the present invention;

[0037] Figure 17 This is a side view of the cross-sectional structure of the adjustment component of the present invention;

[0038] Figure 18 for Figure 15 Enlarged view at point F;

[0039] Figure 19 This is a cross-sectional structural diagram showing the connection relationship between the rotating component and the cleaning component of the present invention;

[0040] Figure 20This is a cross-sectional structural diagram showing the connection relationship between the lifting component, the first moving component, and the second moving component of the present invention.

[0041] Figure 21 This is a cross-sectional structural diagram showing the connection relationship between the first moving component, the second moving component, and the sample holder of the present invention.

[0042] In the diagram: 1. Housing; 2. Camera; 21. Motorized filter wheel; 22. Fluorescent imaging optical path; 23. Fluorescent illumination optical path; 24. First support frame; 25. Second support frame; 26. First L-shaped support plate; 27. LED light; 3. Rotating assembly; 31. Third support plate; 32. First servo motor; 33. Rotating shaft; 34. Connecting block; 4. First threaded plate; 5. Second threaded plate; 6. Lifting assembly; 61. First threaded block; 62. Limit block; 63, slide bar; 64, slide groove; 65, lifting frame; 7, fiber optic sensor; 71, laser contour sensor; 8, drive assembly; 81, second L-shaped support plate; 82, electric push rod; 9, adjusting assembly; 91, rotating block; 92, lifting rod; 93, first inclined block; 94, second inclined block; 95, L-shaped inclined block; 96, inclined groove; 97, T-shaped moving rod; 98, T-shaped moving groove; 99, roller; 10, snap-fit ​​assembly; 101, slide bar 102. Plate; 103. First spring; 104. Locking block; 105. First locking slot; 106. Second locking slot; 107. First electromagnet; 108. First magnet block; 20. Cleaning assembly; 201. Limiting rod; 202. Moving block; 203. Second spring; 204. Second electromagnet; 205. Second magnet block; 206. Third spring; 207. Third electromagnet; 208. Third magnet block; 209. Cleaning block; 30. Collection assembly; 3 01. Mounting plate; 302. Collection frame; 40. First moving component; 401. First adjusting frame; 402. Second servo motor; 403. First threaded rod; 404. Second threaded block; 405. First positioning block; 406. Second adjusting frame; 50. Second moving component; 501. Third servo motor; 502. Second threaded rod; 503. Third threaded block; 504. Second positioning block; 505. Third adjusting frame; 60. Sample holder. Detailed Implementation

[0043] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0044] To address the technical problem of mechanical wear in electric Z-axis modules during long-term use, which affects positioning accuracy, such as... Figures 1-21 As shown, the following preferred technical solutions are provided:

[0045] An automated imaging device for circulating tumor cells includes a housing 1 that supports and fixes various components. A camera 2 is installed inside the housing 1 to receive fluorescence signals and convert them into electrical signals for image acquisition of circulating tumor cells. An electrically driven filter wheel 21 is also installed inside the housing 1 for rapidly switching between filters of different wavelengths, adapting to the device's acquisition requirements for five fluorescence signals (405nm, 488nm, 525nm, 561nm, and 640nm), achieving efficient switching of multi-channel fluorescence imaging. The camera 2 is connected to the electrically driven filter wheel 21. A fluorescence imaging optical path 22 is installed inside the housing 1, which filters and focuses the fluorescence signals emitted by the sample, accurately transmitting the fluorescence signals to the camera 2 to ensure clear imaging. The fluorescence imaging optical path 22 is connected to the motorized filter wheel 21. A fluorescence illumination optical path 23 is fixedly connected to the top of the fluorescence imaging optical path 22. The fluorescence illumination optical path 23 is used to conduct fluorescence excitation light to the sample and, in conjunction with the fluorescence illumination source, excite fluorescent molecules on the sample, such as DAPI and CK markers, to emit fluorescence. A first support frame 24 is provided inside the housing 1. Both the fluorescence imaging optical path 22 and the fluorescence illumination optical path 23 are fixedly connected to the first support frame 24. A second support frame 25 is provided inside the housing 1. A first L-shaped support plate 26 is fixedly connected to the top of the second support frame 25. An LED lamp 27 is installed inside the first L-shaped support plate 26. The LED lamp 27 can serve as a fluorescence excitation source, providing excitation light of wavelengths such as 405nm and 488nm to excite fluorescent molecules in the sample that are labeled with circulating tumor cells to generate fluorescence signals.

[0046] A rotating assembly 3 is provided inside the housing 1. Two first threaded plates 4 and two second threaded plates 5 are slidably connected within the rotating assembly 3. A lifting assembly 6 is provided on one side of the rotating assembly 3, and the lifting assembly 6 is threadedly connected to the two first threaded plates 4 and the two second threaded plates 5 respectively. Figures 4-5 and Figure 8As shown, fiber optic sensors 7 and laser contour sensors 71 are installed on both sides of the lifting assembly 6. The fiber optic sensors 7 are used to monitor the state of the outer walls of the two first threaded plates 4 and the two second threaded plates 5. By changing the reflection of light signals, they determine whether the clumps formed by the mixture of dust and lubricant exceed the normal range. The number of clumps is preset by an external controller for easy comparison. The laser contour sensors 71 can scan the surface contours of the two first threaded plates 4 and the two second threaded plates 5 and compare them with the initial contours to detect the wear of the threaded plate surface during long-term use. A drive assembly 8 is fixedly connected to one side of the rotating assembly 3. An adjustment assembly 9 is slidably connected inside the rotating assembly 3. The drive assembly 8 and the adjustment assembly 9 are rotatably connected. A snap-fit ​​assembly 10 is provided inside the rotating assembly 3 and is connected to the adjustment assembly 9. A cleaning assembly 20 is slidably connected inside the rotating assembly 3. The cleaning assembly 20 can clean the clumps attached to the inner wall of the lifting assembly 6. A collection assembly 30 is provided on one side of the lifting assembly 6.

[0047] During use, when the fiber optic sensor 7 determines, through changes in light signal reflection, that the clumps formed on the outer walls of the two first threaded plates 4 exceed the normal range, the external controller causes the rotating assembly 3 to rotate, causing the lifting assembly 6 to move to the bottom. Then, the external controller causes the locking assembly 10 to disengage from the adjusting assembly 9, and the driving assembly 8 causes the adjusting assembly 9 to descend a short distance. The locking assembly 10 then re-engages with the adjusting assembly 9. During the descent of the adjusting assembly 9, the two first threaded plates 4 gradually move away from the lifting assembly 6, while the two second threaded plates 5 gradually approach but do not contact the lifting assembly 6. At this point, the external controller causes the cleaning assembly 20 to slide out of the rotating assembly 3. Until it is tightly fitted to the inner wall of the lifting assembly 6, the external controller causes the rotating assembly 3 to drive the cleaning assembly 20 to rotate. Through the threaded connection between the cleaning assembly 20 and the lifting assembly 6, the cleaning assembly 20 can easily rotate and descend inside the lifting assembly 6 to clean the inner wall of the lifting assembly 6. During the cleaning process, the clumps that fall fall into the collecting assembly 30 for easy cleaning by the user. After cleaning is completed, the external controller causes the locking assembly 10 to disengage from the adjusting assembly 9 again, and the driving assembly 8 drives the adjusting assembly 9 to rise a short distance. During the rise, the two first threaded plates 4 gradually approach the lifting assembly 6 until the two first threaded plates 4 reconnect with the lifting assembly 6, making it easy to return to the working state.

[0048] During use, when the laser contour sensor 71 detects severe wear on the surfaces of the two first threaded plates 4, the lifting assembly 6 is moved to its lowest position via an external controller. The operation steps are the same as the cleaning process and will not be elaborated further. At this point, the external controller causes the locking assembly 10 to disengage from the adjustment assembly 9. The driving assembly 8 then lowers the adjustment assembly 9 a considerable distance, and the locking assembly 10 re-engages with the adjustment assembly 9. As the adjustment assembly 9 descends, the two first threaded plates 4 gradually move away from the lifting assembly 6, while the two second threaded plates 5 gradually move closer to the lifting assembly 6 until they connect. This allows the two second threaded plates 5 to easily replace the two first threaded plates 4, thus preventing wear on the two first threaded plates 4 from affecting the positioning accuracy in the Z-axis direction. This ensures the stability and data accuracy of multi-channel fluorescence imaging, shortens equipment downtime, ensures the continuity of circulating tumor cell imaging detection, and reduces the cost of consumable replacements and maintenance complexity.

[0049] During the use of the two second threaded plates 5, the fiber optic sensor 7 determines, through changes in light signal reflection, that when the clumps formed on the outer walls of the two second threaded plates 5 exceed the normal range, the lifting assembly 6 is moved to the bottom via an external controller. The operation steps are the same as the cleaning process and will not be elaborated further. At this point, the external controller causes the locking assembly 10 to disengage from the adjusting assembly 9, and the driving assembly 8 drives the adjusting assembly 9 to rise a short distance. Then, the locking assembly 10 re-engages with the adjusting assembly 9. During the rising of the adjusting assembly 9, the two second threaded plates 5 gradually move away from the lifting assembly 6, while the two first threaded plates 4 gradually approach but do not contact the lifting assembly 6. Then, the inner wall of the lifting assembly 6 is cleaned by the cleaning component 20. The cleaning process is the same as the previous cleaning process, so it will not be described in detail here. After cleaning, the locking component 10 is de-engaged from the adjusting component 9 by the external controller, and the adjusting component 9 is lowered by the driving component 8. During the descent, the two second threaded plates 5 gradually approach the lifting assembly 6 until the two second threaded plates 5 are reconnected to the lifting assembly 6, so as to return to the working state. The top of the lifting assembly 6 is fixedly connected to the first moving component 40, the top of the first moving component 40 is fixedly connected to the second moving component 50, and the sample bracket 60 is fixedly connected inside the second moving component 50.

[0050] like Figures 4-15 and Figures 18-19As shown, the rotating assembly 3 includes a third support plate 31 fixedly connected to the housing 1. A first servo motor 32 is installed inside the third support plate 31. A rotating shaft 33 is fixedly connected to the output end of the first servo motor 32. A connecting block 34 is fixedly connected to one side of the third support plate 31. The rotating shaft 33 is rotatably connected to the connecting block 34. Two first threaded plates 4 and two second threaded plates 5 are slidably connected to the rotating shaft 33. The two first threaded plates 4 and two second threaded plates 5 are tightly fitted to the inner wall of the rotating shaft 33, which can prevent impurities from entering the rotating shaft 33 and facilitate the horizontal sliding of the two first threaded plates 4 and two second threaded plates 5 within the rotating shaft 33.

[0051] like Figures 4-8 and Figure 20 As shown, the lifting assembly 6 includes a first threaded block 61 that is threadedly connected to two first threaded plates 4 and two second threaded plates 5 respectively. Two limiting blocks 62 are fixedly connected to one side of the third support plate 31. Slide grooves 64 are fixedly connected to both sides of the first threaded block 61. A slide rod 63 is fixedly connected to one side of the third support plate 31. The slide grooves 64 and the slide rods 63 are slidably connected. Lifting frames 65 are fixedly connected to the top of the two slide grooves 64.

[0052] like Figure 4 and Figure 6 As shown, the drive assembly 8 includes a second L-shaped support plate 81 fixedly connected to one side of the third support plate 31, and an electric push rod 82 is installed on one side of the second L-shaped support plate 81.

[0053] like Figures 4-6 , Figure 9 and Figures 11-15 As shown, the adjustment assembly 9 includes a rotating block 91 rotatably connected to the movable end of the electric push rod 82. A lifting rod 92 is fixedly connected to the bottom of the rotating block 91. The lifting rod 92 is slidably connected to the rotating shaft 33. Several first inclined blocks 93 are connected to the outer ring of the lifting rod 92. Several second inclined blocks 94 are fixedly connected to one side of each of the two second threaded plates 5. The first inclined blocks 93 and the second inclined blocks 94 are in contact. A T-shaped moving rod 97 is fixedly connected to one side of each of the first inclined blocks 93 and the L-shaped inclined blocks 95. A T-shaped moving groove 98 is opened on one side of each of the second inclined blocks 94 and the inclined groove 96. The T-shaped moving rod 97 is slidably connected to the T-shaped moving groove 98, which can easily prevent the T-shaped moving rod 97 from disengaging from the T-shaped moving groove 98 during movement. Rollers 99 are rotatably connected to both sides of the T-shaped moving rod 97. The rollers 99 are rollingly connected to the T-shaped moving groove 98. The rollers 99 can reduce the wear generated when the T-shaped moving rod 97 is connected to the T-shaped moving groove 98.

[0054] like Figure 12 and Figure 14As shown, the snap-fit ​​assembly 10 includes two slide plates 101 slidably connected inside the rotating shaft 33. Two first springs 102 are fixedly connected to one side of each slide plate 101, and the other end of each first spring 102 is fixedly connected to the inner wall of the rotating shaft 33. A snap-fit ​​block 103 is fixedly connected to one side of each slide plate 101. Two first snap-fit ​​slots 104 and a second snap-fit ​​slot 105 are respectively opened on the outer ring of the lifting rod 92. A first electromagnet 106 is installed on the inner wall of the rotating shaft 33. A first magnet block 107 is fixedly connected to one side of each slide plate 101.

[0055] like Figure 10 , Figure 15 and Figures 17-18 As shown, the cleaning assembly 20 includes two limiting rods 201 fixedly connected to the rotating shaft 33. Each limiting rod 201 has a sliding block 202 slidably connected to its outer wall. A rubber pad is provided on the top of each sliding block 202 to facilitate cushioning and reduce the impact force during resetting. The sliding block 202 is slidably connected to the inner wall of the rotating shaft 33. A second spring 203 is fixedly connected to the top of the sliding block 202, and the other end of the second spring 203 is fixedly connected to the inner wall of the rotating shaft 33. Two second electromagnets 204 are installed inside the rotating shaft 33. A second magnet block 205 is fixedly connected to one side of the movable block 202. A third spring 206 is fixedly connected to one side of the movable block 202. A cleaning block 209 is slidably connected to one side of the movable block 202. A third electromagnet 207 is installed on one side of the movable block 202. A third magnet block 208 is fixedly connected to one side of the cleaning block 209. A movable plate is fixedly connected to one side of the cleaning block 209. The movable plate is slidably connected to the movable block 202 and can limit the movement of the cleaning block 209. A thread is provided on one side of the cleaning block 209 so that it can be threadedly connected to the first threaded block 61 during cleaning.

[0056] like Figure 4 and Figure 7 As shown, the collection component 30 includes a mounting plate 301 fixedly connected to one side of the limiting block 62, and a collection frame 302 fixedly connected to one side of each of the two mounting plates 301, with the two collection frames 302 fitting together.

[0057] During use, when the fiber optic sensor 7 determines, through changes in light signal reflection, that the clumps formed on the outer walls of the two first threaded plates 4 exceed the normal range, the external controller activates the first servo motor 32 to drive the rotating shaft 33 and the two first threaded plates 4 to rotate. Through the threaded engagement between the two first threaded plates 4 and the first threaded block 61, the first threaded block 61 and the slide 64 move to their lowest point, causing the bottom of the slide 64 to engage with the top of the limiting block 62. Subsequently, the external controller activates the first electromagnet 106 to attract the first magnet block 107, causing the slide plate 101 and the locking block 103 to move. This causes the locking block 103 to disengage from the first locking slot 104, simultaneously compressing the first spring 102. The external controller then... The electric push rod 82 extends, causing the rotating block 91 and the lifting rod 92 to descend a short distance. Then, the first spring 102 resets the rod, causing the locking block 103 to engage with the second locking groove 105 on the outer ring of the lifting rod 92. During the descent of the lifting rod 92, the L-shaped inclined block 95 presses against the inclined groove 96. At this time, the T-shaped moving rod 97 slides in the T-shaped moving groove 98, and the roller 99 rolls in the T-shaped moving groove 98, causing the two first threaded plates 4 to gradually move away from the inner wall of the first threaded block 61. The first inclined block 93 presses against the second inclined block 94. At this time, the T-shaped moving rod 97 slides in the T-shaped moving groove 98, and the roller 99 rolls in the T-shaped moving groove 98, causing the two second threaded plates 5 to gradually approach but not contact the inner wall of the first threaded block 61.

[0058] At this time, through an external controller, the second electromagnet 204 attracts the second magnet block 205, and the third electromagnet 207 repels the third magnet block 208, causing the third magnet block 208 to drive the cleaning block 209 to fit tightly against the inner wall of the first threaded block 61. At the same time, the third spring 206 is stretched. At this time, through an external controller, the first servo motor 32 drives the rotating shaft 33 and the cleaning block 209 to rotate. Through the threaded connection between the cleaning block 209 and the first threaded block 61, the cleaning block 209 can easily rotate and descend within the first threaded block 61, thereby cleaning the inner wall of the first threaded block 61. During the cleaning process, the clumps that fall off fall into the collection frame 302 for easy cleaning by the user later.

[0059] During the descent of the first threaded block 61, the second spring 203 is stretched. When the cleaning reaches the bottom of the first threaded block 61, the third electromagnet 207 is de-energized by the external controller, causing the third spring 206 to drive the cleaning block 209 to reset. At the moment of reset, the second spring 203 resets, driving the cleaning block 209 to reset upward. This process continues for multiple cycles to avoid jamming and prevent excessive wear on the two first threaded plates 4 due to excessive buildup. After cleaning, the external controller causes the locking block 103 to disengage from the second locking groove 105 on the outer ring of the lifting rod 92. The electric push rod 82 shortens the distance that drives the rotating block 91 and the lifting rod 92 to rise a short distance. During the rise, the L-shaped inclined block 95 pushes the inclined groove 96. At this time, the T-shaped moving rod 97 slides in the T-shaped moving groove 98, and the roller 99 rolls in the T-shaped moving groove 98, causing the two first threaded plates 4 to gradually approach the first threaded block 61 until the two first threaded plates 4 reconnect with the first threaded block 61, facilitating a return to the working state.

[0060] During use, when the laser contour sensor 71 detects severe wear on the surfaces of the two first threaded plates 4, the external controller moves the first threaded block 61 to its lowest position. The operation steps are the same as the cleaning process and will not be elaborated further. At this point, the external controller releases the locking block 103 from the first locking slot 104, and the electric push rod 82 extends, causing the rotating block 91 and the lifting rod 92 to descend a significant distance. The locking block 103 then engages with the other first locking slot 104. As the lifting rod 92 descends, the L-shaped inclined block 95 presses against the inclined groove 96, causing the two first threaded plates 4 to gradually move away from the first threaded plate. The first inclined block 93 presses against the second inclined block 94, causing the two second threaded plates 5 to gradually approach the first threaded block 61 until they connect with it. This allows the two second threaded plates 5 to easily replace the two first threaded plates 4, thus avoiding the impact of wear on the two first threaded plates 4 on the positioning accuracy in the Z-axis direction. This ensures the stability and data accuracy of multi-channel fluorescence imaging, while also shortening the equipment's maintenance downtime, ensuring the continuity of circulating tumor cell imaging detection, and reducing the cost of consumable replacement and maintenance complexity.

[0061] During the use of the two second threaded plates 5, the fiber optic sensor 7 determines, through changes in light signal reflection, that when the clumps formed on the outer walls of the two second threaded plates 5 exceed the normal range, the external controller causes the first threaded block 61 to move to the bottom. The operation steps are the same as the cleaning process, and will not be elaborated here. At this time, the external controller causes the locking block 103 to disengage from the first locking slot 104, and the electric push rod 82 shortens, causing the rotating block 91 and the lifting rod 92 to rise a short distance. Then, the locking block 103 engages with the second locking slot 105 on the outer ring of the lifting rod 92. During the rising process of the lifting rod 92, the two second... The threaded plate 5 gradually moves away from the first threaded block 61, and the two first threaded plates 4 gradually approach but do not contact the first threaded block 61. Then, the inner wall of the lifting assembly 6 is cleaned by the cleaning assembly 20. The cleaning process is the same as the previous cleaning process, so it will not be described in detail here. After cleaning, the locking block 103 is disengaged from the first locking slot 104 by the external controller, and the electric push rod 82 extends to drive the rotating block 91 and the lifting rod 92 to descend a short distance. During the descent, the two second threaded plates 5 gradually approach the first threaded block 61 until the two second threaded plates 5 reconnect with the first threaded block 61, so as to return to the working state.

[0062] To address the technical problem of samples within the sample holder 60 being difficult to move in the X and Y axis directions, such as... Figure 2 , Figures 20-21 As shown, the following preferred technical solutions are provided:

[0063] like Figure 2 , Figures 20-21 As shown, the first moving component 40 includes a first adjusting frame 401 fixedly connected to the top of the lifting frame 65. A second servo motor 402 is installed on one side of the first adjusting frame 401. A first threaded rod 403 is fixedly connected to the output end of the second servo motor 402. The first threaded rod 403 is rotatably connected to the first adjusting frame 401. A second threaded block 404 is threadedly connected to the outer wall of the first threaded rod 403. The second threaded block 404 is slidably connected to the first adjusting frame 401. A second adjusting frame 406 is fixedly connected to the top of the second threaded block 404. A first positioning block 405 is fixedly connected to the bottom of the second adjusting frame 406. The first positioning block 405 is slidably connected to the first adjusting frame 401.

[0064] Figures 20-21As shown, the second moving component 50 includes a third servo motor 501 mounted on one side of the second adjusting frame 406. The output end of the third servo motor 501 is fixedly connected to a second threaded rod 502. The second threaded rod 502 is rotatably connected to the second adjusting frame 406. A third threaded block 503 is threadedly connected to the outer wall of the second threaded rod 502. The third threaded block 503 is slidably connected to the second adjusting frame 406. A third adjusting frame 505 is fixedly connected to the top of the third threaded block 503. A second positioning block 504 is fixedly connected to the bottom of the third adjusting frame 505. The second positioning block 504 is slidably connected to the second adjusting frame 406. A sample holder 60 is fixedly connected inside the third adjusting frame 505.

[0065] In use, the second servo motor 402 drives the first threaded rod 403 to rotate, causing the second threaded block 404 and the second adjustment frame 406 to move. The third servo motor 501 drives the second threaded rod 502 to rotate, causing the third threaded block 503 and the third adjustment frame 505 to move, thereby facilitating the movement of the sample in the sample holder 60 in the X and Y axis directions.

[0066] In use, the separated and stained chip or magnetic bead samples are first placed in the sample holder 60, which is supported by the lifting component 6, the first moving component 40, and the second moving component 50. After setting the sample information and imaging area through the accompanying software, the first moving component 40 and the second moving component 50 drive the sample to move in the X and Y axes. At the same time, through the cooperation between the rotating component 3, the two first threaded plates 4, and the lifting component 6, the sample holder 60 is moved up and down in the Z axis. With the help of the image sharpness judgment algorithm, fast focusing of 100-200ms is achieved. After focusing, the fluorescence imaging optical path 22 emits excitation light of five wavelengths, including 405nm and 488nm, which are switched by the electric filter wheel to the corresponding filter. After the sample is illuminated by the light sheet, the excited fluorescence signal is transmitted to the imaging component via the optical path. The imaging component is existing technology and is not shown in the figure for ease of demonstration. Multi-channel imaging is completed with an exposure time of 200ms. The embedded real-time image recognition algorithm works synchronously to identify suspected circulating tumor cells based on the multi-channel signals and record their XYZ coordinates. Then, the objective lens switching module switches to the 40X objective lens within 1.5 seconds. Through the cooperation of the rotating component 3, the two first threaded plates 4, the lifting component 6, the first moving component 40 and the second moving component 50, the suspected circulating tumor cell location is precisely located for fine scanning. If the recognition confidence is moderate, the process is paused and awaits manual review. The final image is stored in multi-channel TIFF format for analysis and counting by the accompanying software.

[0067] After the chip completes the separation and staining of circulating tumor cells, it is placed on the sample holder 60. The sample information is entered into the software, the upper left corner is placed in the center of the field of view, and the start button is clicked to let the device complete the subsequent imaging work. It automatically shifts, focuses, and images. After the imaging is completed, the process of identifying circulating tumor cells is completed by clicking the analysis button.

[0068] After the magnetic beads complete the separation and staining of circulating tumor cells, the magnetic bead liquid is dropped into the well plate and placed on the sample holder 60. The well plate information is selected in the software, the sample information is entered, and the imaging well plate is positioned. After selection, the device automatically positions the imaging 0 point for imaging, automatically shifts, focuses, and images. After completion, click to analyze to complete the identification process of circulating tumor cells.

[0069] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0070] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An automated imaging device for circulating tumor cells, comprising a housing (1), characterized in that: A camera (2) is installed inside the housing (1). An electric filter wheel (21) is installed inside the housing (1). The camera (2) is connected to the electric filter wheel (21). A fluorescence imaging optical path (22) is installed inside the housing (1). The fluorescence imaging optical path (22) is connected to the electric filter wheel (21). A fluorescence illumination optical path (23) is fixedly connected to the top of the fluorescence imaging optical path (22). A first support frame (24) is provided inside the housing (1). Both the fluorescence imaging optical path (22) and the fluorescence illumination optical path (23) are fixedly connected to the first support frame (24). A second support frame (25) is provided inside the housing (1). A first L-shaped support plate (26) is fixedly connected to the top of the second support frame (25). An LED light (27) is installed inside the first L-shaped support plate (26). A rotating assembly (3) is provided inside the housing (1). Two first threaded plates (4) and two second threaded plates (5) are slidably connected inside the rotating assembly (3). A lifting assembly (6) is provided on one side of the component (3). The lifting assembly (6) is threadedly connected to two first threaded plates (4) and two second threaded plates (5) respectively. A fiber optic sensor (7) and a laser profile sensor (71) are installed on both sides of the lifting assembly (6). A drive assembly (8) is fixedly connected to one side of the rotating assembly (3). An adjustment assembly (9) is slidably connected inside the rotating assembly (3). The drive assembly (8) and the adjustment assembly (9) are rotatably connected. A snap-fit ​​assembly (10) is provided inside the rotating assembly (3). The snap-fit ​​assembly (10) is connected to the adjustment assembly (9). A cleaning assembly (20) is slidably connected inside the rotating assembly (3). A collection assembly (30) is provided on one side of the lifting assembly (6). A first moving assembly (40) is fixedly connected to the top of the lifting assembly (6). A second moving assembly (50) is fixedly connected to the top of the first moving assembly (40). A sample holder (60) is fixedly connected inside the second moving assembly (50).

2. The automated imaging device for circulating tumor cells according to claim 1, characterized in that: The rotating assembly (3) includes a third support plate (31) fixedly connected to the housing (1). A first servo motor (32) is installed in the third support plate (31). A rotating shaft (33) is fixedly connected to the output end of the first servo motor (32). A connecting block (34) is fixedly connected to one side of the third support plate (31). The rotating shaft (33) is rotatably connected to the connecting block (34). Two first threaded plates (4) and two second threaded plates (5) are slidably connected to the rotating shaft (33).

3. An automated imaging device for circulating tumor cells according to claim 2, characterized in that: The lifting assembly (6) includes a first threaded block (61) that is threadedly connected to two first threaded plates (4) and two second threaded plates (5) respectively. Two limit blocks (62) are fixedly connected to one side of the third support plate (31). Slide grooves (64) are fixedly connected to both sides of the first threaded block (61). A slide rod (63) is fixedly connected to one side of the third support plate (31). The slide grooves (64) and slide rods (63) are slidably connected. Lifting frames (65) are fixedly connected to the top of the two slide grooves (64).

4. An automated imaging device for circulating tumor cells according to claim 2, characterized in that: The drive assembly (8) includes a second L-shaped support plate (81) fixedly connected to one side of the third support plate (31), and an electric push rod (82) is installed on one side of the second L-shaped support plate (81).

5. An automated imaging device for circulating tumor cells according to claim 4, characterized in that: The adjusting assembly (9) includes a rotating block (91) rotatably connected to the movable end of the electric push rod (82). A lifting rod (92) is fixedly connected to the bottom of the rotating block (91). The lifting rod (92) is slidably connected to the rotating shaft (33). Several first inclined blocks (93) are connected to each other on the outer ring of the lifting rod (92). Several second inclined blocks (94) are fixedly connected to one side of each of the two second threaded plates (5). The first inclined blocks (93) and the second inclined blocks (94) are in contact. Several L-shaped inclined blocks (95) are connected to each other on the outer ring of the lifting rod (92). The first threaded plate (4) is fixedly connected with a slanted groove (96), and the L-shaped slanted block (95) fits into the slanted groove (96). The first slanted block (93) and the L-shaped slanted block (95) are fixedly connected with a T-shaped moving rod (97) on one side. The second slanted block (94) and the slanted groove (96) are provided with a T-shaped moving groove (98) on one side. The T-shaped moving rod (97) and the T-shaped moving groove (98) are slidably connected. Rollers (99) are rotatably connected on both sides of the T-shaped moving rod (97). The rollers (99) and the T-shaped moving groove (98) are tumbledly connected.

6. An automated imaging device for circulating tumor cells according to claim 5, characterized in that: The snap-fit ​​assembly (10) includes two slide plates (101) slidably connected inside the rotating shaft (33). Two first springs (102) are fixedly connected to one side of each slide plate (101), and the other end of each first spring (102) is fixedly connected to the inner wall of the rotating shaft (33). A snap-fit ​​block (103) is fixedly connected to one side of each slide plate (101). Two first slots (104) and a second slot (105) are respectively opened on the outer ring of the lifting rod (92). A first electromagnet (106) is installed on the inner wall of the rotating shaft (33). A first magnet block (107) is fixedly connected to one side of each slide plate (101).

7. An automated imaging device for circulating tumor cells according to claim 2, characterized in that: The cleaning assembly (20) includes two limiting rods (201) fixedly connected inside the rotating shaft (33). The outer walls of the two limiting rods (201) are slidably connected to moving blocks (202). The moving blocks (202) are slidably connected inside the rotating shaft (33). The top of the moving blocks (202) is fixedly connected to a second spring (203), and the other end of the second spring (203) is fixedly connected to the inner wall of the rotating shaft (33). Two second electromagnets (204) are installed inside the rotating shaft (33). A second magnet block (205) is fixedly connected inside the moving blocks (202). A third spring (206) is fixedly connected to one side of the moving blocks (202). A cleaning block (209) is slidably connected to one side of the moving blocks (202). A third electromagnet (207) is installed on one side of the moving blocks (202). A third magnet block (208) is fixedly connected to one side of the cleaning block (209).

8. An automated imaging device for circulating tumor cells according to claim 3, characterized in that: The collection component (30) includes a mounting plate (301) fixedly connected to one side of the limiting block (62), and a collection frame (302) fixedly connected to one side of each of the two mounting plates (301), with the two collection frames (302) fitting together.

9. An automated imaging device for circulating tumor cells according to claim 3, characterized in that: The first moving component (40) includes a first adjusting frame (401) fixedly connected to the top of the lifting frame (65). A second servo motor (402) is installed on one side of the first adjusting frame (401). A first threaded rod (403) is fixedly connected to the output end of the second servo motor (402). The first threaded rod (403) is rotatably connected to the first adjusting frame (401). A second threaded block (404) is threadedly connected to the outer wall of the first threaded rod (403). The second threaded block (404) is slidably connected to the first adjusting frame (401). A second adjusting frame (406) is fixedly connected to the top of the second threaded block (404). A first positioning block (405) is fixedly connected to the bottom of the second adjusting frame (406). The first positioning block (405) is slidably connected to the first adjusting frame (401).

10. An automated imaging device for circulating tumor cells according to claim 9, characterized in that: The second moving component (50) includes a third servo motor (501) installed on one side of the second adjusting frame (406). The output end of the third servo motor (501) is fixedly connected to a second threaded rod (502). The second threaded rod (502) is rotatably connected to the second adjusting frame (406). The outer wall of the second threaded rod (502) is threadedly connected to a third threaded block (503). The third threaded block (503) is slidably connected to the second adjusting frame (406). The top of the third threaded block (503) is fixedly connected to a third adjusting frame (505). The bottom of the third adjusting frame (505) is fixedly connected to a second positioning block (504). The second positioning block (504) is slidably connected to the second adjusting frame (406). A sample holder (60) is fixedly connected inside the third adjusting frame (505).

Images