Pesticide preparation for improving heat resistance of agrostis stolonifera and application

Abstract

The invention relates to the technical field of pesticides, in particular to a pesticide preparation for improving heat resistance of agrostis stolonifera and application. The active ingredients of the pesticide preparation comprise chitosan and diethyl aminoethyl hexanoate, the concentration of the chitosan is 0.1 g / L, and the concentration of the diethyl aminoethyl hexanoate is 0.1 mmol / L. The diethyl aminoethyl hexanoate and the chitosan are compounded, so that the heat resistance of the agrostis stolonifera can be synergistically improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pesticides, in particular to a pesticide preparation for improving the heat resistance of Agrostis stolonifera and application thereof. BACKGROUND

[0002] High temperature is one of the most critical environmental factors affecting plant growth and geographical distribution. In recent years, the huge greenhouse gas emissions caused by human activities have led to a year-on-year increase in global temperature, which has greatly affected the growth and yield of economic plants worldwide. High temperature also has an adverse effect on the growth and management of turfgrass, resulting in a significant decline in turf quality in summer and an increase in turf maintenance costs. With the gradual increase in summer temperature across the country and the increase in artificial management and maintenance costs, how to improve the heat tolerance of cool-season turfgrass has become a problem that agricultural workers need to solve urgently.

[0003] Agrostis stolonifera L. is a perennial cool-season turfgrass of the Poaceae family and the Agrostis genus, and its growth temperature is between 18℃ and 24℃. Agrostis stolonifera L. can produce a low, fine, and well-textured carpet lawn, and is an excellent cool-season turfgrass suitable for fine sports turf such as golf course greens or tennis courts, and can also be used in parks, factories, government agencies, schools, urban green spaces, and is a good material for slope protection and soil conservation. However, since its optimal growth temperature range is 18℃ to 24℃, in transitional and warm climate regions, the summer high temperature exceeding 30℃ leads to a decline in turf quality. Therefore, it is urgent to explore cultivation measures and methods to improve the heat tolerance of Agrostis stolonifera L. in its cultivation.

[0004] In the prior art, plant growth regulators for improving plant heat tolerance mainly include salicylic acid (SA), abscisic acid (ABA), brassinosteroids (BRs), jasmonates (JAs), and polyamines (PAs), etc. However, the stability and sustained effect of these single regulators are poor, which limits their large-scale application in turf maintenance and management. Therefore, it is particularly important to explore new and efficient composite formulations with long-lasting effects for improving the maintenance and management of Agrostis stolonifera L. turfgrass under high temperature stress in summer. SUMMARY

[0005] In order to solve the defects in the prior art, the present application provides a pesticide preparation for improving the heat resistance of Agrostis stolonifera and application thereof. The present application creatively finds that the combination of amaranth fat and chitosan can synergistically improve the heat resistance of Agrostis stolonifera.

[0006] To achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0007] In a first aspect, the present application provides a pesticide preparation for improving the heat tolerance of creeping bentgrass, wherein the active ingredients of the pesticide preparation comprise chitosan and diaminophosphinyl acetic acid (DA-6), the concentration of the chitosan is 0.1 g / L, and the concentration of the DA-6 is 0.1 mmol / L.

[0008] Preferably, the pesticide preparation further comprises an auxiliary material, and the auxiliary material comprises any one or more of a solvent, an adjuvant, a wetting agent, a dispersing agent, an antifreezing agent, a thickening agent, a disintegrating agent, a filler, and a nutrient element.

[0009] Preferably, the pesticide preparation is in any one of a water agent, an emulsifiable concentrate, a microemulsion, an emulsion, and a suspension.

[0010] The active ingredients in the composition of the present application can be purchased from a commercial channel for compounding, or a preparation containing the active ingredients can be purchased for direct compounding.

[0011] In a second aspect, the present application provides the use of the pesticide preparation in improving the heat tolerance of creeping bentgrass.

[0012] Preferably, the application site of the pesticide preparation is the leaf of a plant.

[0013] Preferably, the application amount of the pesticide preparation is 1 L / m 2 .

[0014] Preferably, the heat tolerance temperature is 35℃ during the day and 30℃ at night.

[0015] Preferably, the heat tolerance is to improve the photosynthetic performance, the leaf water content, and the chlorophyll content.

[0016] Preferably, the heat tolerance is to reduce the electrolyte permeation and the accumulation of malondialdehyde.

[0017] The present application has the following beneficial effects:

[0018] DA-6 is a highly bioactive cytokine and belongs to plant hormones. Spraying DA-6 on the leaves at an appropriate concentration can effectively improve the heat tolerance of creeping bentgrass, significantly alleviate the leaf aging caused by high temperature, reduce the oxidative damage of the cell membrane under high-temperature stress, and improve the net photosynthetic rate and the stability of the cell membrane.

[0019] Chitosan (CTS) is a deacetylation product of chitin, which is one of the most abundant natural polysaccharides in nature. CTS is an important osmotic regulator in plant cells and an important antioxidant polysaccharide. Exogenous CTS with a suitable concentration can effectively enhance the systemic defense function of creeping bentgrass and thus improve the heat resistance of the plant. Under high-temperature stress, spraying CTS on leaves can significantly alleviate water loss, reduce cell membrane oxidative damage caused by high temperature, and improve the photochemical efficiency, net photosynthetic rate and cell membrane stability of leaves.

[0020] In the pesticide preparation of the present application, chitosan (CTS) and DA-6 are compounded to improve the heat resistance of creeping bentgrass. DA-6 belongs to plant hormones, while CTS is an important cell osmotic regulator. The two have a synergistic effect when compounded at a suitable concentration. Therefore, the DA-6 and CTS composition provided by the present application can effectively reduce the content of malondialdehyde, a product of cell membrane lipid peroxidation, protect the function of biological membranes, increase the content of chlorophyll, photochemical efficiency and net photosynthetic rate of plants, and enhance the water retention capacity of cells, thereby significantly improving the heat resistance of creeping bentgrass and effectively alleviating leaf senescence caused by high temperature. The composition components selected by the technical solution provided by the present application have low production cost and low price compared with other similar products, and are suitable for large-area popularization and application. In addition, the application effect of the composition is not affected by the application species, and has a broad-spectrum synergistic effect. DETAILED DESCRIPTION

[0021] In order to enable those skilled in the art to better understand the technical solutions of the present application, the present application will be further described in detail below in combination with specific embodiments.

[0022] The raw materials or adjuvants used in the embodiments of the present application can be purchased from the market.

[0023] Example 1: Effect of different CTS and DA-6 concentration combinations on the heat resistance physiology of creeping bentgrass

[0024] (1) Test material: PA-4, a widely cultivated commercial variety in production, was used as the test material.

[0025] (2) Material cultivation: The sterilized creeping bentgrass seeds (10 g / m 2 ) were evenly sown in a square container with a length of 20 cm, a width of 15 cm and a height of 6 cm, and quartz sand was used as the substrate. The seeds were first germinated in distilled water for 7 days, and then Hoagland solution was used instead of distilled water for irrigation. The seedlings were further cultivated in Hoagland solution. The temperature of the plant growth chamber was 25℃ during the day and 20℃ at night, with a duration of 12 hours each. The relative humidity was 70%, and the light intensity was 700 μmol m -2 s -1When the creeping bentgrass is 35 days old, the materials with consistent growth are selected for heat stress treatment.

[0026] (3) Test design: The following 10 combinations of CTS (g / L) + DA-6 (mmol / L) concentrations are set: 0+0 (control), 0.05+0.05, 0.05+0.1, 0.1+0.05, 0.1+0.1, 0.1+0, 0+0.1, 0.1+0.2, 0.2+0.1, 0.2+0.2, a total of 10 treatments. The purity of CTS and DA-6 used is greater than 98%. CTS and DA-6 are directly dissolved in distilled water, and are used immediately after preparation. Distilled water without CTS and DA-6 is used as a control treatment. Each treatment is set up with 4 independent replicates (i.e. 4 pots per treatment).

[0027] (4) Application of combined additives and heat stress treatment: Each treatment is sprayed for 3 consecutive days before heat stress. The control group is sprayed with distilled water, and the spraying site is the plant leaves. The spraying amount is 1 L / m 2 . After the continuous spraying is completed, high temperature treatment is started. The spraying is supplemented every week to ensure the effect. The heat stress is set at a temperature of 35℃ during the day and 30℃ at night, each for 12 hours. Hoagland solution is used to keep the plants from being subjected to drought stress during the high temperature stress. The stress is stopped when the leaves show obvious wilting. The heat stress is ended on the 14th day.

[0028] (5) Test results:

[0029] Table 1. Effect of different CTS and DA-6 concentration combinations on heat tolerance physiological indicators of creeping bentgrass

[0030]

[0031]

[0032] Note: Different letters in each column (same indicator) indicate significant differences (P<0.05)

[0033] Table 2. Analysis of different concentration effect membership functions

[0034] CTS (g / L) + DA-6 (mmol / L) Membership function value Ranking 0.1+0.1 0.92 1 0+0.1 0.58 2 0.1+0 0.54 3 0.1+0.2 0.53 4 0.1+0.05 0.51 5 0.05+0.05 0.50 6 0.05+0.1 0.48 7 0.2+0.2 0.47 8 0.2+0.1 0.46 9 0+0 0.08 10

[0035] As can be seen from Table 1, high temperature stress leads to the decrease of photosynthetic efficiency of stolons, the decrease of relative water content, the decrease of chlorophyll content, the increase of accumulation of malondialdehyde (MDA) and the decrease of cell membrane stability (the increase of electrolyte permeability). After the application of the CTS+DA-6 combination preparation, the plant under high temperature stress can maintain a high photosynthetic efficiency, the relative water content and the chlorophyll content of the leaf can be maintained at a significantly higher level, and the electrolyte permeability can be reduced. The effect of the CTS+DA-6 combination preparation is significantly better than that of single CTS or single DA-6. Therefore, the physiological indexes of different treatments further prove that, compared with the control (0+0), single CTS (0.1+0) and single DA-6 (0+0.1), the combination of 0.1 g / L CTS+0.1 mmol / L DA-6 has a great advantage in improving the heat tolerance of stolons. And through the analysis of the membership function (Table 2), it can be known that the best preparation combination is 0.1 g / L CTS+0.1 mmol / L DA-6, which has a great advantage in relieving the wilting of stolon leaves and improving the heat tolerance of stolons.

[0036] Application effect of the best CTS+DA-6 combination on different varieties of stolons in Example 2

[0037] (1) Test material: four different stolon varieties of 'PA-1', 'Penncross', 'Lofts L-93' and 'Penneagle' were selected.

[0038] (2) Material cultivation: the same as in Example 1.

[0039] (3) Test design: in order to observe the effect of the best seed soaking concentration combination 0.1 g / L CTS+0.1 mmol / L DA-6 on different stolon varieties, four widely used stolon varieties were selected to be sprayed with deionized water (Water, control group) and the best concentration combination (0.1 g / L CTS+0.1 mmol / L DA-6) for 3 days before heat stress. The control group was sprayed with distilled water, the spraying site was the leaf of the plant, and the spraying amount was 1 L / m 2 . After the continuous spraying, high temperature treatment was started, and 1 spraying was added every week to ensure the effect. The heat stress was set at 35℃ during the day and 30℃ at night, respectively for 12 hours. Hoagland solution was used to keep the plants from drought stress during the high temperature stress. The stress was stopped when the leaf wilting was observed, and the heat stress was ended on the 14th day.

[0040] (4) Germination index statistics: the same as in Example 1.

[0041] (5) Test results:

[0042] Table 3. The application effect of the optimal CTS combined with DA-6 treatment on different varieties of St. Augustine under high temperature stress

[0043]

[0044] Note: "*" indicates significant difference (P<0.05) between two different treatments (Water and CTS+DA-6) of the same variety and the same index

[0045] As can be seen from Table 3, after spraying the optimal concentration combination of 0.1 g / L CTS+0.1 mmol / L DA-6, the photosynthetic inhibition, water loss, electrolyte exosmosis and leaf chlorosis (decrease of chlorophyll content) caused by high temperature were significantly alleviated, indicating that the optimal concentration combination can significantly improve the heat tolerance of four different varieties of St. Augustine, and also indicating that the optimal concentration combination has a broad-spectrum effect on improving the heat tolerance of St. Augustine.

[0046] The above is only the preferred embodiment of the present application, and it should be noted that the above preferred embodiment should not be regarded as a limitation of the present application, and the protection scope of the present application should be limited by the scope defined by the claims. For ordinary skilled persons in the art, several improvements and refinements can be made without departing from the spirit and scope of the present application, and these improvements and refinements should also be regarded as the protection scope of the present application.

Claims

1. A pesticide preparation for improving heat tolerance of creeping bentgrass, characterized by, The active ingredient of the pesticide preparation comprises chitosan and amine brilliant, the concentration of the chitosan is 0.1 g / L, and the concentration of the amine brilliant is 0.1 mmol / L.

2. The agricultural chemical preparation according to claim 1, characterized by The pesticide preparation further comprises an auxiliary material, and the auxiliary material comprises any one or more of a solvent, an auxiliary agent, a wetting agent, a dispersing agent, an anti-freezing agent, a thickening agent, a disintegrating agent, a filler, and a nutrient element.

3. The agricultural chemical preparation according to claim 1, characterized by, The dosage form of the pesticide preparation is any one of a water agent, an emulsifiable concentrate, a microemulsion, an emulsion in water, and a suspension concentrate.

4. Application of the pesticide preparation in claims 1-3 to improving heat resistance of creeping bentgrass.

5. Use according to claim 4, characterized in that, The application site of the pesticide preparation is a plant leaf.

6. Use according to claim 4, characterized in that, The application amount of the pesticide preparation is 1 L / m 2 .

7. Use according to claim 4, characterized in that, The heat resistance temperature is 35 DEG C in the daytime and 30 DEG C at night.

8. Use according to claim 4, characterized in that, The heat resistance is improving photosynthetic performance, increasing leaf water content, and increasing chlorophyll content.

9. Use according to claim 4, characterized in that, The heat resistance is reducing electrolyte permeation and malondialdehyde accumulation.

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