Camellia oil, torreya grandis oil and walnut oil matched edible formula oil for health care and preparation method thereof

Abstract

The invention belongs to the technical field of edible oil, and particularly relates to health-care edible formula oil matched with camellia oil, torreya grandis oil and walnut oil and a preparation method of the health-care edible formula oil. The edible formula oil is prepared from the following components in percentage by mass: 42%-45% of camellia oil, 21%-22% of torreya grandis oil, 21%-22% of walnut oil, purple perilla seed oil, sea buckthorn fruit oil, grape seed oil, royal jelly freeze-dried powder, a rosemary extract and wall-broken Chinese wolfberry fruit powder. During preparation, the camellia oil, the torreya grandis oil and the walnut oil are prepared through low-temperature cold pressing and a specific refining technology and then mixed according to the proportion of 2: 1: 1 to form base oil, the purple perilla seed oil, the sea buckthorn fruit oil, the grape seed oil, the royal jelly freeze-dried powder, the wall-broken Chinese wolfberry fruit powder and the rosemary extract are sequentially added into the base oil, stirring and mixing are conducted, and filtering is conducted to obtain the product. The edible formula oil is good in nutrition synergy, can effectively retain active ingredients through the technology, has the effects of strengthening oxidation resistance, cardiovascular protection and immunoregulation, and is suitable for being eaten by people needing daily health care.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of edible oil, in particular to a health-care formula oil of camellia oil, torreya grandis oil and walnut oil and a preparation method thereof. BACKGROUND

[0002] With the improvement of national health consciousness, woody edible oil rich in active ingredients such as unsaturated fatty acids, vitamins and phytosterols has gradually become an important choice for healthy diet. Camellia oil is rich in sterols and vitamin E and has the potential to regulate blood lipids; torreya grandis oil contains unique squalene, which can enhance cell activity; walnut oil is known for its high content of alpha-linolenic acid, which is beneficial to the health of the nervous system. However, the nutritional components of single woody edible oil are relatively single, which is difficult to meet the demand of human body for multiple active substances, so the composite formula oil has become a research hotspot.

[0003] In the prior art, there are many deficiencies in the composite edible oil: firstly, there are defects in the process, most of which use high-temperature pressing or refining process, resulting in a large loss of heat-sensitive active ingredients such as sterols, squalene and vitamin E; secondly, the formula is unreasonable, the ratio of base oil lacks scientific synergy, and most of the added ingredients are chemical antioxidants, which poses a safety hazard; thirdly, the function is single, focusing on single nutritional supplementation, and unable to achieve multi-dimensional synergy of antioxidant, immune regulation and cardiovascular protection. In addition, in the preparation of existing composite oil, solid functional ingredients are prone to agglomeration, and the filtering process often leads to the loss of effective ingredients, affecting the stability and health care function of the product. Therefore, it is a technical problem to be solved in the field to develop a woody edible oil composite formula that retains active ingredients by low-temperature process, has scientific formula synergy, and has safety and multi-dimensional health care function. SUMMARY

[0004] The present application aims to provide a health-care formula oil of camellia oil, torreya grandis oil and walnut oil and a preparation method thereof to solve the problems raised in the background art. The edible formula oil of the present application is configured from camellia oil, torreya grandis oil, walnut oil, perilla seed oil, sea buckthorn fruit oil, grape seed oil, royal jelly freeze-dried powder, rosemary extract and broken wolfberry powder. It not only maximizes the retention of active ingredients such as sterols in camellia oil, squalene in torreya grandis oil, alpha-linolenic acid and vitamin E in walnut oil through low-temperature cold pressing and moderate refining process, but also realizes nutritional synergy through scientific ratio. The base oil is formulated in a ratio of 2:1:1, which forms an antioxidant and cardiovascular protection closed loop with the Omega-3 in perilla seed oil, carotenoids in sea buckthorn fruit oil and proanthocyanidins in grape seed oil. The royal jelly freeze-dried powder and broken wolfberry powder synergistically enhance the immune regulation ability. At the same time, the use of natural rosemary extract instead of chemical antioxidants improves the safety of edible oil, and the low-temperature control and precise filtration during preparation ensure that the active ingredients are not lost and evenly dispersed, so that the finished product has multi-dimensional health care functions such as antioxidant, blood lipid regulation and immune enhancement.

[0005] The application provides a health-care formula oil of camellia oil, torreya grandis oil and walnut oil, which is prepared from the following components in mass percentage: camellia oil 42-44%, torreya grandis oil 21-22%, walnut oil 21-22%, perilla seed oil 3-5%, sea buckthorn fruit oil 2-3%, grape seed oil 5-7%, royal jelly freeze-dried powder 0.5-1%, rosemary extract 0.1-0.2% and broken wall wolfberry powder 1-2%.

[0006] Preferably, the preparation method of the camellia oil comprises the following specific steps:

[0007] (1) selecting mature and full oil-tea camellia seeds, removing the oil-tea camellia seeds with mildew, insect damage and damage through artificial and mechanical combined screening, and adopting an oil-tea camellia seed special shelling machine for shelling treatment; the shelled oil-tea camellia seed kernels are placed in a low-temperature air drying box and dried at 40-50 ℃ for 6-8 hours, so that the water content of the oil-tea camellia seed kernels is controlled to 5-8%;

[0008] (2) the screened, shelled and dried oil-tea camellia seeds are put into a spiral cold press with a press chamber temperature ≤60 ℃ for low-temperature cold pressing; the pressing pressure of the low-temperature cold pressing is adjusted in stages, the initial pressure is 15-20 MPa, and the pressure is maintained for 5 minutes, then the pressure is gradually increased to 30-40 MPa within 20-30 minutes, and the camellia crude oil is obtained through solid-liquid separation after pressing;

[0009] (3) the camellia crude oil is first subjected to normal pressure rough filtration through a 100-mesh food-grade filter cloth to remove the suspended solid impurities in the camellia crude oil, then the filtered camellia crude oil is sent into a disc centrifuge for centrifugal separation at a speed of 3000-4000 r / min for 15-20 minutes to further remove the fine colloidal particles in the camellia crude oil, and the clarified camellia crude oil is obtained;

[0010] (4) 3-5% of 80-85 ℃ deionized water is added to the clarified camellia crude oil, and the mixture is stirred at a speed of 100-150 r / min for 30 minutes and then left to stand for 2 hours, so that the colloidal precipitate is removed through centrifugal separation, and the degummed clarified camellia crude oil is obtained;

[0011] (5) the degummed clarified camellia crude oil is cooled to 30-40 ℃, 10% of an edible sodium hydroxide solution is added, and the mixture is stirred at a speed of 80-100 r / min for 20 minutes and then left to stand for 4 hours, so that the soap foot is separated through a plate and frame filter, and the deacidified clarified camellia crude oil is obtained;

[0012] (6) To the clarified Camellia oleifera Abel crude oil after deacidification, add 2%-3% of activated bleaching earth by weight of oil, and stir at a speed of 60-80 r / min under the conditions of vacuum degree 0.08-0.09 MPa and 40-50℃ for 30 minutes to make the bleaching earth fully adsorb the pigments and part of the fat-soluble impurities in the oil, and then remove the bleaching earth by filtering through 200-mesh filter cloth to obtain the clarified Camellia oleifera Abel crude oil after bleaching;

[0013] (7) Send the clarified Camellia oleifera Abel crude oil after bleaching into a deodorization tower, and pass in food-grade nitrogen gas at a flow rate of 1-1.5 L / min under the conditions of vacuum degree 0.095 MPa and 100-120℃ for 2-3 hours of continuous deodorization, and then perform fine filtration through 0.5-μm multi-layer plant fiber filter cloth to obtain Camellia oil with clear color and no odor.

[0014] Preferably, the preparation method of the Torreya grandis fruit oil specifically comprises the following steps:

[0015] (1) Select mature Torreya grandis fruits, remove the Torreya grandis fruits with empty shells, insect damage and mold change by manual and mechanical combined screening, and perform shelling treatment by using a Torreya grandis special shelling machine to obtain Torreya grandis kernels, soak the Torreya grandis kernels in warm water at 40-45℃ for 10-15 minutes, and then gently rub the Torreya grandis kernels by using a drum rubbing peeling machine to remove the kernel coats, and then transfer the peeled Torreya grandis kernels into a vacuum drying box, dry the peeled Torreya grandis kernels under the conditions of vacuum degree 0.07-0.08 MPa and 45-50℃ for 4-5 hours, and control the water content of the Torreya grandis kernels to 4%-6%;

[0016] (2) Put the Torreya grandis fruits after screening, shelling, peeling and drying into a horizontal spiral cold press machine with a presser temperature ≤55℃ to perform low-temperature cold pressing, and adjust the pressing pressure of the low-temperature cold pressing in stages, the initial pressure is 15-20 MPa, and the initial pressure is maintained for 5 minutes, and then gradually increase the pressure to 25-30 MPa within 15-20 minutes, and then perform solid-liquid separation by using a spiral separator to obtain Torreya grandis crude oil;

[0017] (3) First, perform pressure filtration of the Torreya grandis crude oil by using 150-mesh food-grade filter cloth, and then transfer the filtered Torreya grandis crude oil into a tubular centrifuge, and centrifuge the Torreya grandis crude oil at a speed of 5000-6000 r / min for 10-15 minutes to obtain clarified Torreya grandis crude oil;

[0018] (4) Add 2%-4% of 30-35℃ deionized water by weight of oil to the clarified Torreya grandis crude oil, stir at a speed of 80-100 r / min for 20 minutes, and then stand still for 1.5 hours, and then remove the gum precipitate by centrifugal separation to obtain the degummed clarified Torreya grandis crude oil;

[0019] (5) The degummed and clarified Torreya grandis L. crude oil is transferred into a supercritical carbon dioxide deacidification device, under the conditions of 40-45°C and vacuum degree of 0.08 MPa, supercritical carbon dioxide is introduced at a flow rate of 2-3 L / h, and free fatty acids are removed by intermolecular forces to obtain the deacidified and clarified Torreya grandis L. crude oil;

[0020] (6) 1.5%-2.5% of modified activated clay by weight of the oil is added to the deacidified and clarified Torreya grandis L. crude oil, under the conditions of vacuum degree of 0.085 MPa and 35-45°C, the mixture is stirred at a speed of 80-100 r / min for 25 minutes to allow the activated clay to fully adsorb pigments and fat-soluble impurities, and then the activated clay is removed by filtration through a 200-mesh filter cloth to obtain the decolorized and clarified Torreya grandis L. crude oil, wherein the modified activated clay is activated clay activated at 60°C;

[0021] (7) The decolorized and clarified Torreya grandis L. crude oil is sent into a deodorization tower, under the conditions of vacuum degree of 0.096 MPa and 90-100°C, food-grade nitrogen is introduced at a flow rate of 1-1.5 L / min, and the deodorization is continued for 1.5-2 hours, and then the deodorized Torreya grandis L. oil is refined by filtration through a 0.4-μm multi-layer plant fiber filter cloth to obtain the Torreya grandis L. oil with clear color and retained unique nutty flavor.

[0022] Preferably, the preparation method of the walnut oil comprises the following specific steps:

[0023] (1) Mature walnuts are selected, and walnuts with empty shells, insect damage and mold are removed by manual and mechanical screening, and the walnuts are shelled by using a walnut shelling machine, and the obtained walnut kernels are placed in a low-temperature air drying oven, and the surface residual brown septum is removed by hand, and the walnut kernels with the removed septum are dried in a low-temperature air drying oven at 40-45°C for 3-4 hours to control the water content of the walnut kernels to 3%-5%;

[0024] (2) The screened, shelled, septum-removed and dried walnuts are put into a vertical spiral cold press with a temperature of the press chamber ≤50°C to perform low-temperature cold pressing, and the pressing pressure of the low-temperature cold pressing is adjusted in stages, the initial pressure is 10-15 MPa, and the initial pressure is maintained for 5 minutes, and then the pressure is gradually increased to 20-25 MPa within 10 minutes, and then the pressed walnuts are subjected to solid-liquid separation by using a spiral sedimentation type solid-liquid separator to obtain walnut crude oil;

[0025] (3) The walnut crude oil is first subjected to pressure filtration through a 200-mesh food-grade filter cloth, and then the filtered Torreya grandis L. crude oil is sent into a ceramic membrane filtration system, and the Torreya grandis L. crude oil is refined by filtration through a 0.2-μm ceramic membrane under the conditions of 30-35°C and membrane surface flow rate of 1.5-2 m / s to further remove colloidal particles, and the clarified Torreya grandis L. crude oil is obtained;

[0026] (4) adding 1-3% of 30℃ deionized water to the clarified walnut crude oil, stirring at a speed of 60-80 r / min for 15 minutes and then standing for 1 hour, removing the gum precipitate by centrifugal separation to obtain degummed clarified walnut crude oil;

[0027] (5) transferring the degummed clarified walnut crude oil into a molecular distillation deacidification device, removing free fatty acids by molecular distillation technology under the conditions of 45-50℃, vacuum degree 0.09 MPa, and control of the feeding speed at 50-80 mL / h, to obtain deacidified clarified walnut crude oil;

[0028] (6) adding 1-2% of food-grade activated carbon to the deacidified clarified walnut crude oil, stirring at a speed of 60-80 r / min for 20 minutes under the conditions of vacuum degree 0.08 MPa and 30-40℃, and then removing the activated carbon by filtration through 300-mesh filter cloth to obtain decolorized clarified walnut crude oil;

[0029] (7) transferring the decolorized clarified walnut crude oil into a deodorization tower, continuously deodorizing for 1-1.5 hours under the conditions of vacuum degree 0.097 MPa and 80-90℃, with the flow rate of food-grade argon being 1-1.2 L / min, and then performing fine filtration through 0.3 μm multi-layer plant fiber filter cloth to obtain walnut oil.

[0030] Preferably, the particle size of the royal jelly freeze-dried powder is 200-300 mesh, and the particle size of the broken wolfberry powder is 201-400 mesh.

[0031] Preferably, the preparation method of the edible formula oil comprises the following steps:

[0032] S1. mixing camellia oil, torreya grandis oil and walnut oil at a ratio of 2:1:1 to form base oil;

[0033] S2. sequentially adding perilla seed oil, sea buckthorn fruit oil and grape seed oil to the base oil, keeping the temperature ≤40℃ and stirring to mix, to obtain composite oil;

[0034] S3. dispersing royal jelly freeze-dried powder and broken wolfberry powder in the composite oil, keeping the temperature ≤40℃ and stirring to mix evenly, to obtain blended oil;

[0035] S4. adding rosemary extract to the blended oil and stirring to mix evenly, to obtain edible formula oil.

[0036] Preferably, the stirring speed in S2 is 100-200 r / min, and the stirring time is 10-15 minutes, and the stirring speed in S3 is 150-200 r / min, and the stirring time is 20-30 minutes.

[0037] Preferably, the rosemary extract in S4 needs to be dissolved in grape seed oil in advance and then added into the blending oil.

[0038] Preferably, the edible formula oil obtained in S4 needs to be filtered through a multi-layer plant fiber filter with a filtering precision of 35-75 μm.

[0039] Preferably, the storage container of the edible formula oil is a light-proof brown glass bottle or a stainless steel container with inert treatment on the inner wall, and the storage temperature is 10-20℃ and the relative humidity is ≤65%.

[0040] Preferably, the perilla seed oil, sea buckthorn fruit oil and grape seed oil all need to be filtered through a 300-mesh filter cloth.

[0041] Preferably, 5%-7% of the total mass percentage of the grape seed oil is composed of the grape seed oil directly added into the base oil in S2 and the grape seed oil used for dissolving the rosemary extract in S4, wherein the mass of the grape seed oil used for dissolving the rosemary extract in S4 accounts for 5%-15% of the total mass of the grape seed oil, and the mass of the grape seed oil directly added in S2 accounts for 85%-95% of the total mass of the grape seed oil.

[0042] Compared with the prior art, the health-care camellia oil torreya grandis oil walnut oil formula edible oil and the preparation method thereof have the following beneficial effects:

[0043] 1. The active ingredients of the edible formula oil are more fully retained, and the nutritional potency is higher. Through low-temperature cold pressing and moderate refining process, the heat-sensitive active ingredients in each oil can be maximally retained, which lays a material foundation for the health-care effect of the edible formula oil.

[0044] 2. The edible formula oil can realize multi-dimensional health-care function. Through scientific proportioning, the nutritional synergy is realized. The base oil is compounded according to the proportion of 2:1:1 of camellia oil: torreya grandis oil: walnut oil, which forms an antioxidant and cardiovascular protection closed loop with the Omega-3 in perilla seed oil, the carotenoids in sea buckthorn fruit oil and the proanthocyanidins in grape seed oil, can synergistically reduce blood lipids and scavenge free radicals. At the same time, the freeze-dried royal jelly powder and the broken wall lycium barbarum powder can synergistically enhance the immune regulation ability through small molecule active substances, which solves the problem of single function of the existing composite oil and realizes the effects of antioxidant, blood lipid regulation and immune enhancement.

[0045] 3. The natural rosemary extract is used to replace the traditional chemical antioxidant, which avoids the chemical reagent residue and significantly improves the edible safety. At the same time, the solid ingredients in the formula can be uniformly dispersed in the oil through precise particle size control and low-temperature dispersion process, which is suitable for long-term consumption of people who need daily health care. DETAILED DESCRIPTION

[0046] The following describes preferred embodiments of the present application, and it should be understood that the embodiments are for better explaining the present application and are not intended to limit the present application.

[0047] Example 1, a health-care camellia oil torreya grandis oil walnut oil formula edible oil, consisting of the following components in mass percentage: camellia oil 42%-44%, torreya grandis oil 21%-22%, walnut oil 21%-22%, perilla seed oil 3%-5%, sea buckthorn fruit oil 2%-3%, grape seed oil 5%-7%, royal jelly freeze-dried powder 0.5%-1%, rosemary extract 0.1%-0.2%, and broken wolfberry powder 1%-2%.

[0048] The health-care camellia oil torreya grandis oil walnut oil formula edible oil according to the present application preferably consists of the following components in mass percentage: camellia oil 42%, torreya grandis oil 21%, walnut oil 21%, perilla seed oil 5%, sea buckthorn fruit oil 2.4%, grape seed oil 7%, royal jelly freeze-dried powder 0.5%, rosemary extract 0.1%, and broken wolfberry powder 1%.

[0049] The preparation method of the health-care camellia oil torreya grandis oil walnut oil formula edible oil according to the present application comprises the following steps:

[0050] (1) Select mature and full oil tea seeds, remove moldy, worm-eaten and damaged seeds through artificial and mechanical combined screening, and then shell the oil tea seeds with an oil tea seed special shelling machine. After that, place the oil tea seed kernels in a low-temperature air-drying oven at 40℃ for drying for 6 hours, and control the water content to be 8%. Put the dried oil tea seeds into a spiral cold press machine with a press chamber temperature of 60℃, and press at an initial pressure of 15MPa for 5 minutes, and then gradually increase the pressure to 30MPa within 20 minutes. After pressing, separate the solid and liquid to obtain camellia crude oil. Filter the camellia crude oil with 100-mesh filter cloth, and then centrifuge at 3000r / min for 15 minutes to obtain clear camellia crude oil. Add 3% of 80℃ deionized water to the clear camellia crude oil, stir at 100r / min for 30 minutes, and then stand for 2 hours. Centrifugal degumming is performed to obtain degummed clear camellia crude oil. Cool the degummed clear camellia crude oil to 30℃, add 10% sodium hydroxide solution, stir at 80r / min for 20 minutes, and then stand for 4 hours. Perform plate and frame filtration to remove acid and obtain acid-removed clear camellia crude oil. Add 2% of activated clay to the acid-removed clear camellia crude oil, and stir at 60r / min under the conditions of a vacuum degree of 0.08MPa and a temperature of 40℃ for 30 minutes. Filter with 200-mesh filter cloth to obtain decolorized clear camellia crude oil. Transfer the decolorized clear camellia crude oil into a deodorization tower, and deodorize under the conditions of a vacuum degree of 0.095MPa and a temperature of 100℃ by passing in 1L / min of nitrogen gas for 2 hours. Perform fine filtration with 0.5μm multi-layer plant fiber filter cloth to obtain camellia oil.

[0051] (2) Select mature Torreya grandis fruits, shell after screening, Torreya grandis seed kernels are soaked in warm water at 40°C for 10 minutes, then the peeled Torreya grandis seed kernels are dried in a vacuum drying box at a vacuum degree of 0.07 MPa and 45°C for 4 hours, the moisture content is controlled to 6%, and dried Torreya grandis fruits are obtained; the dried Torreya grandis fruits are put into a horizontal spiral cold press with a temperature of 55°C, the initial pressure is 15 MPa, and the pressure is maintained for 5 minutes, then the pressure is gradually increased to 25 MPa within 15 minutes, and Torreya grandis crude oil is obtained by spiral separation after pressing; the Torreya grandis crude oil is filtered under pressure through 150-mesh filter cloth, and then centrifuged at 5000 r / min for 10 minutes to obtain clear Torreya grandis crude oil; 2% of deionized water at 30°C by weight of oil is added to the clear Torreya grandis crude oil, stirred at 80 r / min for 20 minutes, and then left to stand for 1.5 hours, and the degummed clear Torreya grandis crude oil is obtained by centrifugal degumming; the degummed clear Torreya grandis crude oil is transferred into a supercritical carbon dioxide deacidification device, 2L / h of supercritical carbon dioxide is introduced for deacidification at 40°C and a vacuum degree of 0.08 MPa, and the deacidified clear Torreya grandis crude oil is obtained; 1.5% of activated modified activated clay at 60°C by weight of oil is added to the deacidified clear Torreya grandis crude oil, stirred at 80 r / min for 25 minutes at a vacuum degree of 0.085 MPa and 35°C, and then filtered through 200-mesh filter cloth to obtain the decolorized clear Torreya grandis crude oil; the decolorized clear Torreya grandis crude oil is transferred into a deodorization tower, 1L / min of nitrogen gas is introduced for deodorization at a vacuum degree of 0.096 MPa and 90°C for 1.5 hours, and then the Torreya grandis oil is obtained by fine filtration through 0.4-μm multi-layer plant fiber filter cloth.

[0052] (3) Select mature walnuts, after screening, remove the shell, manually remove the brown diaphragm on the surface of the walnut kernel, and place the walnut kernel with the removed diaphragm in a low-temperature air-drying oven at 40°C for drying for 3 hours, control the moisture content of the walnut kernel to 5%, obtain dried walnuts, and put the dried walnuts into a vertical spiral cold press with a temperature of 50°C in the press chamber, an initial pressure of 10 MPa, and a duration of 5 minutes, and then gradually increase the pressure to 20 MPa within 10 minutes, press the walnuts, and then separate the walnut kernel from the press by spiral sedimentation to obtain walnut crude oil; filter the walnut crude oil through 200-mesh filter cloth under pressure, and then filter the filtered oil through a 0.2-μm ceramic membrane under the conditions of 30°C and a membrane surface flow rate of 1.5 m / s to obtain clear walnut crude oil; add deionized water with an oil weight of 1% at 30°C to the clear walnut crude oil, stir at 60 r / min for 15 minutes, and then stand for 1 hour, centrifugal degumming to obtain degummed clear walnut crude oil, transfer the degummed clear walnut crude oil into a molecular distillation deacidification device, control the feeding speed to 50 mL / h under the conditions of 45°C and a vacuum degree of 0.09 MPa to deacidify, and obtain deacidified clear walnut crude oil, add food-grade activated carbon with an oil weight of 1% to the deacidified clear walnut crude oil, stir at 60 r / min under the conditions of a vacuum degree of 0.08 MPa and 30°C for 20 minutes to decolorize, and then filter through 300-mesh filter cloth to obtain decolorized clear walnut crude oil; transfer the decolorized clear walnut crude oil into a deodorization tower, deodorize the oil under the conditions of a vacuum degree of 0.097 MPa and 80°C by passing in 1 L / min of argon for 1 hour, and then filter the oil through a 0.3-μm multilayer plant fiber filter cloth to obtain walnut oil.

[0053] (4) Mix camellia oil, torreya grandis oil, and walnut oil in a ratio of 2:1:1 to form base oil; add perilla seed oil, sea buckthorn fruit oil, and grape seed oil (85% of the total mass of grape seed oil) filtered through 300-mesh filter cloth to the base oil, stir at 100 r / min for 10 minutes while maintaining the temperature at 40°C, and obtain a compound oil; disperse 200-250-mesh freeze-dried royal jelly powder and 201-300-mesh broken wolfberry powder in the compound oil, stir at 150 r / min for 20 minutes while maintaining the temperature at 40°C, and obtain a blended oil; dissolve rosemary extract in the remaining 15% of grape seed oil in advance, add the blended oil, stir uniformly, filter through a 35-75-μm multilayer plant fiber filter, and obtain an edible formula oil; store the edible formula oil in a brown glass bottle in the dark, and store the oil at a temperature of 10°C and a relative humidity of 65%.

[0054] Example 2, a health-care camellia oil torreya grandis oil walnut oil blended edible formula oil, consists of the following components in mass percentage: camellia oil 42%-44%, torreya grandis oil 21%-22%, walnut oil 21%-22%, perilla seed oil 3%-5%, sea buckthorn fruit oil 2%-3%, grape seed oil 5%-7%, freeze-dried royal jelly powder 0.5%-1%, rosemary extract 0.1%-0.2%, and broken wolfberry powder 1%-2%.

[0055] The health-care camellia oil, torreya grandis oil and walnut oil combined edible formula oil according to the present application is preferably composed of the following components in mass percentage: camellia oil 44%, torreya grandis oil 22%, walnut oil 22%, perilla seed oil 3%, sea buckthorn fruit oil 2%, grape seed oil 5%, royal jelly freeze-dried powder 0.8%, rosemary extract 0.1%, and broken wall Chinese wolfberry powder 1.1%.

[0056] The preparation method of the health-care camellia oil, torreya grandis oil and walnut oil combined edible formula oil according to the present application comprises the following steps:

[0057] (1) Select mature and full oil tea seeds, remove mildewed, worm-eaten and damaged seeds through artificial and mechanical combined screening, and then shell the oil tea seeds by using an oil tea seed special shelling machine. After that, place the oil tea seed kernels in a low-temperature air-drying box at 50℃ for drying for 8 hours, and control the moisture content to be 5%. Put the dried oil tea seeds into a spiral cold press machine with a press chamber temperature of 58℃, and press at an initial pressure of 20 MPa for 5 minutes, and then gradually increase the pressure to 40 MPa within 30 minutes. After pressing, obtain camellia crude oil through solid-liquid separation. Filter the camellia crude oil through 100-mesh filter cloth, and then centrifuge at 4000 r / min for 20 minutes to obtain clear camellia crude oil. Add 5% of 85℃ deionized water to the clear camellia crude oil, stir at 150 r / min for 30 minutes, and then stand still for 2 hours. Centrifugal degumming is performed to obtain degummed clear camellia crude oil. Cool the degummed clear camellia crude oil to 40℃, add 10% sodium hydroxide solution, stir at 100 r / min for 20 minutes, and then stand still for 4 hours. Perform plate and frame filtration to remove acid to obtain acid-removed clear camellia crude oil. Add 3% of activated clay to the acid-removed clear camellia crude oil, and stir at 80 r / min under the conditions of vacuum degree 0.09 MPa and 50℃ for 30 minutes. Filter through 200-mesh filter cloth to obtain decolorized clear camellia crude oil. Transfer the decolorized clear camellia crude oil into a deodorization tower, and deodorize under the conditions of vacuum degree 0.095 MPa and 120℃ for 3 hours by passing in 1.5 L / min nitrogen gas. Perform fine filtration through 0.5 μm multi-layer plant fiber filter cloth to obtain camellia oil.

[0058] (2) Select mature Torreya grandis fruits, shell after screening, Torreya grandis seed kernels are soaked in warm water at 45°C for 15 minutes, then the peeled Torreya grandis seed kernels are dried in a vacuum drying box at a vacuum degree of 0.08 MPa and 50°C for 5 hours, the moisture content is controlled to be 4%, and dried Torreya grandis fruits are obtained; the dried Torreya grandis fruits are put into a horizontal spiral cold press with a temperature of 53°C, the initial pressure is 20 MPa, and the pressure is maintained for 5 minutes, then the pressure is gradually increased to 30 MPa within 20 minutes, and Torreya grandis crude oil is obtained by spiral separation after pressing; the Torreya grandis crude oil is filtered under pressure through 150-mesh filter cloth, and then centrifuged at 6000 r / min for 15 minutes to obtain clear Torreya grandis crude oil; 4% of deionized water at 35°C by weight of the oil is added to the clear Torreya grandis crude oil, stirred at 100 r / min for 20 minutes, and then left to stand for 1.5 hours, and the degummed clear Torreya grandis crude oil is obtained by centrifugal degumming; the degummed clear Torreya grandis crude oil is transferred into a supercritical carbon dioxide deacidification device, 3L / h of supercritical carbon dioxide is introduced for deacidification at 45°C and a vacuum degree of 0.08 MPa, and the deacidified clear Torreya grandis crude oil is obtained; 2.5% of activated modified activated clay at 60°C by weight of the oil is added to the deacidified clear Torreya grandis crude oil, stirred at 100 r / min for 25 minutes at a vacuum degree of 0.085 MPa and 45°C, and then filtered through 200-mesh filter cloth to obtain the decolorized clear Torreya grandis crude oil; the decolorized clear Torreya grandis crude oil is transferred into a deodorization tower, 1.5L / min of nitrogen is introduced for deodorization at a vacuum degree of 0.096 MPa and 100°C for 2 hours, and then the Torreya grandis oil is obtained by fine filtration through 0.4-μm multi-layer plant fiber filter cloth.

[0059] (3) Select mature walnuts, after screening, remove the shell, manually remove the brown diaphragm on the surface of the walnut kernel, and place the walnut kernel with the removed diaphragm in a low-temperature air drying oven, dry at 45°C for 4 hours, control the moisture content of the walnut kernel to 3%, obtain dried walnuts, and put the dried walnuts into a vertical spiral cold press with a temperature of 48°C in the press chamber, an initial pressure of 15 MPa, and a duration of 5 minutes, and then gradually increase the pressure to 25 MPa within 10 minutes. The walnut crude oil is obtained by screw sedimentation separation after pressing. The walnut crude oil is filtered by 200-mesh filter cloth under pressure, and then filtered by 0.2-μm ceramic membrane under the conditions of 35°C and a membrane surface flow rate of 2 m / s to obtain clear walnut crude oil. Add 3% of the oil weight of 30°C deionized water to the clear walnut crude oil, stir at 80 r / min for 15 minutes, and then stand for 1 hour. Centrifugal degumming is performed to obtain degummed clear walnut crude oil. The degummed clear walnut crude oil is transferred into a molecular distillation deacidification device, and deacidification is performed under the conditions of 50°C and a vacuum degree of 0.09 MPa by controlling the feeding speed to 80 mL / h to obtain deacidified clear walnut crude oil. Add 2% of the oil weight of food-grade activated carbon to the deacidified clear walnut crude oil, stir at 80 r / min for 20 minutes under the conditions of a vacuum degree of 0.08 MPa and 40°C, and then filter through 300-mesh filter cloth to obtain decolorized clear walnut crude oil. The decolorized clear walnut crude oil is transferred into a deodorization tower, and deodorization is performed under the conditions of a vacuum degree of 0.097 MPa and 90°C by introducing 1.2 L / min of argon for 1.5 hours. The walnut oil is obtained by fine filtering through 0.3-μm multi-layer plant fiber filter cloth.

[0060] (4) Mix according to the ratio of camellia oil: torreya grandis oil: walnut oil = 2:1:1 to form a base oil; add filtered perilla seed oil, sea buckthorn fruit oil and grape seed oil (95% of the total mass of grape seed oil) to the base oil in turn, keep the temperature at 38°C, and stir at 200 revolutions / minute for 15 minutes to obtain a compound oil; disperse 350-300 mesh royal jelly freeze-dried powder and 300-400 mesh broken wolfberry powder in the compound oil, keep the temperature at 38°C, and stir at 200 revolutions / minute for 30 minutes to obtain a blended oil; dissolve rosemary extract in the remaining 5% grape seed oil in advance, add it to the blended oil, stir uniformly, and then filter through 35-75-μm multi-layer plant fiber to obtain an edible formula oil; store the edible formula oil in a brown glass bottle in the dark, and store it at a temperature of 20°C and a relative humidity of 63%.

[0061] Example 3, a health-care camellia oil torreya grandis oil walnut oil collocation edible formula oil, consists of the following components in mass percentage: camellia oil 42%-44%, torreya grandis oil 21%-22%, walnut oil 21%-22%, perilla seed oil 3%-5%, sea buckthorn fruit oil 2%-3%, grape seed oil 5%-7%, royal jelly freeze-dried powder 0.5%-1%, rosemary extract 0.1%-0.2%, and broken wolfberry powder 1%-2%;

[0062] The health-care camellia oil, torreya grandis oil and walnut oil combined edible formula oil according to the present application is preferably composed of the following components in mass percentage: camellia oil 43%, torreya grandis oil 21.5%, walnut oil 21.5%, perilla seed oil 3.5%, sea buckthorn fruit oil 2.5%, grape seed oil 5.6%, royal jelly freeze-dried powder 0.75%, rosemary extract 0.15%, and broken cell wolfberry powder 1.5%.

[0063] The preparation method of the health-care camellia oil, torreya grandis oil and walnut oil combined edible formula oil according to the present application comprises the following steps:

[0064] (1) Select mature and full oil tea seeds, remove moldy, worm-eaten and damaged seeds through artificial and mechanical combined screening, and then shell the oil tea seeds by using an oil tea seed special shelling machine. After that, place the oil tea seed kernels in a low-temperature air-drying box at 45℃ for drying for 7 hours, and control the moisture content to be 7%. Put the dried oil tea seeds into a spiral cold press machine with a press chamber temperature of 56℃, and press at an initial pressure of 17 MPa for 5 minutes, and then gradually increase the pressure to 35 MPa within 25 minutes. After pressing, obtain camellia crude oil through solid-liquid separation. Filter the camellia crude oil through 100-mesh filter cloth, and then centrifuge at 3500 r / min for 18 minutes to obtain clear camellia crude oil. Add 4% of 83℃ deionized water to the clear camellia crude oil, and stir at 120 r / min for 30 minutes, and then stand for 2 hours. Centrifugal degumming is performed to obtain degummed clear camellia crude oil. Cool the degummed clear camellia crude oil to 35℃, and add 10% sodium hydroxide solution. Stir at 90 r / min for 20 minutes, and then stand for 4 hours. Perform plate and frame filtration to remove acid to obtain acid-removed clear camellia crude oil. Add 2.5% of activated clay to the acid-removed clear camellia crude oil, and stir at 70 r / min under the conditions of vacuum degree 0.085 MPa and 45℃ for 30 minutes. Filter through 200-mesh filter cloth to obtain decolorized clear camellia crude oil. Transfer the decolorized clear camellia crude oil into a deodorization tower, and deodorize under the conditions of vacuum degree 0.095 MPa and 110℃ for 2.5 hours by passing in 1.25 L / min of nitrogen. Perform fine filtration through 0.5-μm multi-layer plant fiber filter cloth to obtain camellia oil.

[0065] (2) Select mature Torreya grandis fruits, remove the shells after screening, and soak the Torreya grandis seeds in warm water at 42.5°C for 12.5 minutes. Then, remove the skin of the Torreya grandis seeds by rolling and rubbing in a drum. Dry the Torreya grandis seeds with the skin removed in a vacuum drying oven at a vacuum degree of 0.075 MPa and 47.5°C for 4.5 hours, and control the moisture content to 5%. Dry Torreya grandis fruits are obtained. Put the dry Torreya grandis fruits into a horizontal spiral cold press with a temperature of 51°C in the press chamber. The initial pressure is 17 MPa, and the pressure is maintained for 5 minutes. Then, the pressure is gradually increased to 27.5 MPa within 17.5 minutes. Torreya grandis crude oil is obtained by screw separation after pressing. The Torreya grandis crude oil is filtered under pressure through a 150-mesh filter cloth, and then centrifuged at 5500 r / min for 12.5 minutes to obtain clear Torreya grandis crude oil. Add 3% of deionized water at 32.5°C to the clear Torreya grandis crude oil, and stir at 90 r / min for 20 minutes. Then, let it stand for 1.5 hours. Degumming is carried out by centrifugation to obtain degummed clear Torreya grandis crude oil. The degummed clear Torreya grandis crude oil is transferred into a supercritical carbon dioxide deacidification device. Under the conditions of 42.5°C and a vacuum degree of 0.08 MPa, 2.5 L / h of supercritical carbon dioxide is introduced for deacidification to obtain deacidified clear Torreya grandis crude oil. Add 2% of activated modified activated bleaching earth at 60°C to the deacidified clear Torreya grandis crude oil. Stir at 90 r / min for 25 minutes under the conditions of a vacuum degree of 0.085 MPa and 40°C. Then, filter through a 200-mesh filter cloth to obtain decolorized clear Torreya grandis crude oil. The decolorized clear Torreya grandis crude oil is transferred into a deodorization tower. Under the conditions of a vacuum degree of 0.096 MPa and 95°C, 1.25 L / min of nitrogen gas is introduced for deodorization for 1.7 hours. Then, the Torreya grandis oil is obtained by fine filtration through a 0.4-μm multi-layer plant fiber filter cloth.

[0066] (3) Select mature walnuts, after screening, remove the shell, manually remove the brown diaphragm on the surface of the walnut kernel, and place the walnut kernel with the removed diaphragm in a low-temperature air-drying oven at 42°C for drying for 3.6 hours, control the moisture content of the walnut kernel to 4%, obtain dried walnuts, and put the dried walnuts into a vertical spiral cold press with a temperature of 46°C in the press chamber, an initial pressure of 12 MPa, and a duration of 5 minutes, and then gradually increase the pressure to 22 MPa within 10 minutes, press the walnuts, and then separate the walnut kernel from the press by spiral sedimentation to obtain walnut crude oil; filter the walnut crude oil through 200-mesh filter cloth under pressure, and then filter the filtered oil through a 0.2-μm ceramic membrane under the conditions of 32°C and a membrane surface flow rate of 1.7 m / s to obtain clear walnut crude oil; add 2% of the oil weight of deionized water at 30°C to the clear walnut crude oil, stir at 70 r / min for 15 minutes, and then stand for 1 hour, centrifugal degum the clear walnut crude oil to obtain degummed clear walnut crude oil, and then transfer the degummed clear walnut crude oil into a molecular distillation deacidification device, control the feeding speed to 60 mL / h under the conditions of 47°C and a vacuum degree of 0.09 MPa to deacidify the oil, and then obtain deacidified clear walnut crude oil, add 1.5% of the oil weight of food-grade activated carbon to the deacidified clear walnut crude oil, stir at 70 r / min under the conditions of a vacuum degree of 0.08 MPa and 35°C for 20 minutes to decolorize the oil, and then filter the decolorized oil through 300-mesh filter cloth to obtain decolorized clear walnut crude oil; transfer the decolorized clear walnut crude oil into a deodorization tower, deodorize the oil under the conditions of a vacuum degree of 0.097 MPa and 85°C by passing in 1.1 L / min of argon for 1.2 hours, and then filter the deodorized oil through a 0.3-μm multilayer plant fiber filter cloth to obtain walnut oil.

[0067] (4) Mix camellia oil, torreya grandis oil, and walnut oil in a ratio of 2:1:1 to form base oil; add perilla seed oil, sea buckthorn fruit oil, and grape seed oil (90% of the total mass of grape seed oil) filtered through 300-mesh filter cloth to the base oil, stir at 150 rpm for 12.5 minutes while maintaining the temperature at 36°C to obtain compound oil; disperse 220-260-mesh royal jelly freeze-dried powder and 330-380-mesh broken wolfberry powder in the compound oil, stir at 170 rpm for 25 minutes while maintaining the temperature at 36°C to obtain blended oil; dissolve rosemary extract in the remaining 10% of grape seed oil in advance, add the blended oil, stir uniformly, and then filter through a 35-75-μm multilayer plant fiber filter to obtain edible formula oil; store the edible formula oil in a brown glass bottle in the dark at a storage temperature of 15°C and a relative humidity of 61%.

[0068] Example 4, a health-care camellia oil torreya grandis oil walnut oil blended edible formula oil, consists of the following components in mass percentage: camellia oil 42%-44%, torreya grandis oil 21%-22%, walnut oil 21%-22%, perilla seed oil 3%-5%, sea buckthorn fruit oil 2%-3%, grape seed oil 5%-7%, royal jelly freeze-dried powder 0.5%-1%, rosemary extract 0.1%-0.2%, and broken wolfberry powder 1%-2%.

[0069] The health-care camellia oil, torreya grandis oil and walnut oil combined edible formula oil according to the present application is preferably composed of the following components in mass percentage: camellia oil 42.5%, torreya grandis oil 21.25%, walnut oil 21.25%, perilla seed oil 4%, sea buckthorn fruit oil 2.8%, grape seed oil 6.3%, royal jelly freeze-dried powder 0.75%, rosemary extract 0.15%, and broken wall Chinese wolfberry powder 1%.

[0070] The preparation method of the health-care camellia oil, torreya grandis oil and walnut oil combined edible formula oil according to the present application comprises the following steps:

[0071] (1) Select mature and full oil tea seeds, remove moldy, worm-eaten and damaged seeds through artificial and mechanical combined screening, and then shell the oil tea seeds with a special oil tea seed shelling machine. After that, the oil tea seed kernels are dried in a low-temperature air-drying box at 47℃ for 7.5 hours to control the moisture content to 6%. The dried oil tea seeds are then fed into a spiral cold press machine with a press chamber temperature of 55℃. The initial pressure is 18 MPa, and then gradually increased to 37 MPa within 27 minutes. After pressing, the camellia crude oil is obtained through solid-liquid separation. The camellia crude oil is coarsely filtered through 100-mesh filter cloth, and then centrifuged at 3700 r / min for 16 minutes to obtain clear camellia oil. 3.5% of 81℃ deionized water is added to the clear camellia oil, and stirred at 120 r / min for 30 minutes, and then left to stand for 2 hours. The oil is degummed by centrifugation to obtain degummed clear camellia oil. The degummed clear camellia oil is cooled to 32℃, and 10% sodium hydroxide solution is added. The mixture is stirred at 85 r / min for 20 minutes, and then left to stand for 4 hours. The mixture is filtered through a plate and frame filter to obtain deacidified clear camellia oil. 2.2% of activated clay is added to the deacidified clear camellia oil, and stirred at 65 r / min under the conditions of vacuum degree 0.082 MPa and temperature 43℃ for 30 minutes. The mixture is filtered through 200-mesh filter cloth to obtain decolorized clear camellia oil. The decolorized clear camellia oil is transferred into a deodorization tower, and deodorized under the conditions of vacuum degree 0.095 MPa and temperature 105℃ for 2.3 hours by passing in 1.4 L / min nitrogen gas. The mixture is finely filtered through 0.5 μm multi-layer plant fiber filter cloth to obtain camellia oil.

[0072] (2) Select mature Torreya grandis fruits, shell after screening, Torreya grandis kernel is soaked in 41℃ warm water for 10 minutes, then the peeled Torreya grandis kernel is dried in a vacuum drying box at a vacuum degree of 0.072 MPa and 46℃ for 4.2 hours, the moisture content is controlled to 4.3%, and dried Torreya grandis fruits are obtained; the dried Torreya grandis fruits are put into a horizontal spiral cold press machine with a temperature of 50℃ in the press chamber, the initial pressure is 16 MPa, and the pressure is maintained for 5 minutes, then the pressure is gradually increased to 29 MPa within 16 minutes, and Torreya grandis crude oil is obtained by spiral separation after pressing; the Torreya grandis crude oil is filtered by pressure through 150 mesh filter cloth, and then centrifuged at 5300 r / min for 12 minutes to obtain clear Torreya grandis crude oil; 2.5% of 32℃ deionized water by weight of the oil is added to the clear Torreya grandis crude oil, stirred at 85 r / min for 20 minutes, and then left to stand for 1.5 hours, and the degummed clear Torreya grandis crude oil is obtained by centrifugal degumming; the degummed clear Torreya grandis crude oil is transferred into a supercritical carbon dioxide deacidification device, 2.7 L / h of supercritical carbon dioxide is introduced at 42℃ and a vacuum degree of 0.08 MPa to deacidify the clear Torreya grandis crude oil, and the deacidified clear Torreya grandis crude oil is obtained; 1.8% of 60℃ activated modified activated clay by weight of the oil is added to the deacidified clear Torreya grandis crude oil, stirred at 85 r / min for 25 minutes under the conditions of a vacuum degree of 0.085 MPa and 42℃, and then filtered through 200 mesh filter cloth to obtain the decolorized clear Torreya grandis crude oil; the decolorized clear Torreya grandis crude oil is transferred into a deodorization tower, 1.15 L / min of nitrogen is introduced at a vacuum degree of 0.096 MPa and 93℃ to deodorize the clear Torreya grandis crude oil for 1.6 hours, and then the Torreya grandis oil is obtained by fine filtration through 0.4 μm multi-layer plant fiber filter cloth.

[0073] (3) Select mature walnuts, shell after screening, manually remove the brown diaphragm on the surface of walnut kernels, and place the walnut kernels with the removed diaphragm in a low-temperature air-drying box and dry at 41°C for 3.2 hours to control the moisture content of the walnut kernels to 4.5%, obtaining dried walnuts. The dried walnuts are put into a vertical spiral cold press with a press chamber temperature of 50°C, an initial pressure of 11 MPa, and a duration of 5 minutes, and then gradually increased to 21 MPa within 10 minutes. The walnut crude oil is obtained by screw settling separation after pressing. The walnut crude oil is pressure-filtered through 200-mesh filter cloth, and then precision-filtered through a 0.2-μm ceramic membrane at 32°C and a membrane surface flow rate of 1.6 m / s to obtain clear walnut crude oil. Deionized water with an oil weight of 1.5% at 30°C is added to the clear walnut crude oil, stirred at 65 r / min for 15 minutes, and then left to stand for 1 hour. The degummed clear walnut crude oil is obtained by centrifugal degumming. The degummed clear walnut crude oil is transferred into a molecular distillation deacidification device, and deacidification is carried out at 46°C and a vacuum degree of 0.09 MPa by controlling the feeding speed to 70 mL / h to obtain deacidified clear walnut crude oil. Food-grade activated carbon with an oil weight of 1.25% is added to the deacidified clear walnut crude oil, and the mixture is stirred at 65 r / min for 20 minutes at a vacuum degree of 0.08 MPa and 32°C, and then filtered through 300-mesh filter cloth to obtain decolorized clear walnut crude oil. The decolorized clear walnut crude oil is transferred into a deodorization tower, and deodorization is carried out at a vacuum degree of 0.097 MPa and 83°C by passing in 1.05 L / min of argon for 1.15 hours. The deodorized walnut oil is obtained by precision-filtration through 0.3-μm multi-layer plant fiber filter cloth.

[0074] (4) The camellia oil, Japanese nut oil and walnut oil are mixed in a ratio of 2:1:1 to form a base oil; the perilla seed oil, sea buckthorn fruit oil and grape seed oil (88% of the total mass of grape seed oil) filtered through 300-mesh filter cloth are sequentially added to the base oil, the temperature is maintained at 35°C, and the mixture is stirred at 120 rpm for 14 minutes to obtain a compound oil; the 260-290-mesh royal jelly freeze-dried powder and 230-280-mesh broken wolfberry powder are dispersed in the compound oil, the temperature is maintained at 35°C, and the mixture is stirred at 160 rpm for 23 minutes to obtain a blended oil; the rosemary extract is dissolved in the remaining 12% of grape seed oil, added to the blended oil, and stirred uniformly, and then filtered through 35-75-μm multi-layer plant fiber to obtain an edible formula oil; and the edible formula oil is stored in a brown glass bottle in the dark at a storage temperature of 13°C and a relative humidity of 60%.

[0075] Specifically, to verify the efficacy of the edible formula oil prepared in this invention in terms of antioxidation, lipid regulation, and immune enhancement, 50 SPF-grade SD rats weighing 200±20g were randomly divided into 5 groups of 10 each, serving as the control group, experimental group 1, experimental group 2, experimental group 3, and experimental group 4, respectively. The control group was fed a basal diet, experimental group 1 was fed a basal diet supplemented with 5% of the edible formula oil prepared in Example 1, experimental group 2 was fed a basal diet supplemented with 5% of the edible formula oil prepared in Example 2, and experimental group 3 was fed a basal diet supplemented with 5% of the edible formula oil prepared in Example 3. The basal diet of the edible formula oil and the experimental group 4 were fed with the basal diet of the edible formula oil prepared in Example 4, with 5% added. After acclimatization in an environment of 22°C and 55% humidity for 3 days, the rats in each group were fed experimentally according to their groups, with free access to food and water. The food intake was recorded daily and the rats were weighed once a week. After 45 days of feeding, the rats were fasted for 12 hours, and blood was collected from the orbital cavity. The serum was separated by centrifugation at 3000 r / min for 15 minutes. After the rats were sacrificed, the liver, thymus and spleen were quickly removed, weighed and the organ index was calculated, where the organ index = organ weight / body weight × 100%.

[0076] Specialized reagent kits were used to detect the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), immunoglobulin G (IgG), and high-density lipoprotein cholesterol (HDL-C) in the serum of each group. The thymus index and spleen index were also calculated. SOD, GSH, and MDA were used to reflect antioxidant function; total cholesterol, triglycerides, LDL-C, and HDL-C were used to reflect lipid regulation function; and the thymus index, immunoglobulin G, and spleen index were used to reflect immune enhancement function. The results of antioxidant function testing for each group are shown in Table 1, the results of lipid regulation function testing for each group are shown in Table 2, and the results of immune enhancement function testing for each group are shown in Table 3.

[0077] Table 1:

[0078]

[0079]

[0080] Table 2:

[0081]

[0082] Table 3:

[0083]

[0084] According to the experimental data, the serum SOD and GSH-Px activities of the rats in the experimental groups 1-4 are significantly higher than those of the control group, and the MDA content is significantly lower than that of the control group, indicating that the edible formula oil of the application can enhance the antioxidant enzyme activity of the body, reduce the lipid peroxidation product, and has significant antioxidant effect; the serum TC, TG and LDL-C levels of the rats in the experimental groups 1-4 are significantly lower than those of the control group, and the HDL-C level is significantly higher than that of the control group, indicating that the edible formula oil of the application can effectively regulate blood lipid metabolism and reduce the risk of cardiovascular disease; the thymus index, spleen index and serum IgG content of the rats in the experimental groups 1-4 are significantly higher than those of the control group, indicating that the edible formula oil of the application can promote the development of immune organs and improve the humoral immune level; accordingly, the edible formula oil prepared by the application can synergistically exert the multi-dimensional health care functions of antioxidant, blood lipid regulation and immune enhancement through the active ingredients retained by scientific proportioning and low-temperature process.

[0085] The above is only the preferred embodiment of the application and is not intended to limit the application. The formula and preparation process of the application can be variously modified and changed by those skilled in the art. Any modification, equivalent replacement, improvement, etc. within the spirit and principle of the application shall be included in the protection scope of the application.

Claims

1. A health care formula oil of camellia oil, torreya grandis oil and walnut oil, characterized in that, consists of the following components in percentage by mass camellia oil 42%-44%, torreya grandis oil 21%-22%, walnut oil 21%-22%, perilla oil 3%-5%, sea buckthorn oil 2%-3%, grape seed oil 5%-7%, royal jelly freeze-dried powder 0.5%-1%, rosemary extract 0.1%-0.2%, and broken wolfberry powder 1%-2%.

2. The health care formula oil of camellia oil, torreya grandis oil and walnut oil according to claim 1, characterized in that, The preparation method of the camellia oil, the torreya grandis oil, and the walnut oil is as follows: (1) The preparation method of the camellia oil is as follows: after screening, shelling, and drying, the oil-tea camellia seed is put into a presser with a temperature ≤60℃ in the pressing chamber to be cold-pressed at low temperature, and the solid-liquid separation is performed to obtain camellia crude oil; the camellia crude oil is coarsely filtered through 100-mesh filter cloth and centrifuged at 3000-4000 r / min to obtain clear camellia crude oil; the clear camellia crude oil is sequentially degummed by adding deionized water at 80-85℃, deacidified by adding 10% sodium hydroxide solution at 30-40℃, decolored by adding activated clay under vacuum at 40-50℃, and deodorized by passing nitrogen gas under vacuum at 100-120℃, and then filtered to obtain the camellia oil. (2) The preparation method of the torreya grandis oil is as follows: after screening, shelling, peeling, and drying, the torreya grandis fruit is put into a presser with a temperature ≤55℃ in the pressing chamber to be cold-pressed at low temperature, and the solid-liquid separation is performed to obtain torreya grandis crude oil; the torreya grandis crude oil is pressure-filtered through 150-mesh filter cloth and then centrifuged at 5000-6000 r / min to obtain clear torreya grandis crude oil; the clear torreya grandis crude oil is sequentially degummed by adding deionized water at 30-35℃, deacidified by passing supercritical carbon dioxide under vacuum at 40-45℃, decolored by adding modified activated clay under vacuum at 35-45℃, and deodorized by passing nitrogen gas under vacuum at 90-100℃, and then filtered to obtain the torreya grandis oil. (3) The preparation method of the walnut oil is as follows: after screening, shelling, removing septum, and drying, the walnut is put into a presser with a temperature ≤50℃ in the pressing chamber to be cold-pressed at low temperature, and the solid-liquid separation is performed to obtain walnut crude oil; the walnut crude oil is filtered through 200-mesh filter cloth and then precision-filtered through a 0.2-μm ceramic membrane to obtain clear walnut crude oil; the clear walnut crude oil is sequentially degummed by adding deionized water at 30℃, deacidified by molecular distillation under vacuum at 45-50℃, decolored by adding activated carbon under vacuum at 30-40℃, and deodorized by passing argon gas under vacuum at 80-90℃, and then filtered to obtain the walnut oil.

3. The health care formula oil of camellia oil, torreya grandis oil and walnut oil according to claim 1, characterized in that, The particle size of the royal jelly freeze-dried powder is 200-300 mesh, and the particle size of the broken wolfberry powder is 201-400 mesh.

4. The health care formula oil of camellia oil, torreya grandis oil and walnut oil according to claim 1, characterized in that, The preparation method of the edible formula oil includes the following steps: S1. The camellia oil, the torreya grandis oil, and the walnut oil are mixed in a ratio of 2:1:1 to form a base oil; S2. The perilla oil, the sea buckthorn oil, and the grape seed oil are sequentially added to the base oil, the temperature is kept ≤40℃, and the mixture is stirred to obtain a composite oil; S3. The royal jelly freeze-dried powder and the broken wolfberry powder are dispersed in the composite oil, the temperature is kept ≤40℃, and the mixture is stirred uniformly to obtain a blended oil; S4. The rosemary extract is added to the blended oil, and the mixture is stirred uniformly to obtain the edible formula oil.

5. The health care formula oil of camellia oil, torreya grandis oil and walnut oil according to claim 4, characterized in that, The stirring speed of the stirring mixing in S2 is 100-200 rpm, and the stirring time is 10-15 minutes; the stirring speed of the stirring uniformity in S3 is 150-200 rpm, and the stirring time is 20-30 minutes.

6. The health care formula oil of camellia oil, torreya grandis oil and walnut oil according to claim 4, characterized in that, The rosemary extract in S4 needs to be dissolved in grape seed oil in advance before being added into the blending oil.

7. The health care formula oil of camellia oil, torreya grandis oil and walnut oil according to claim 4, characterized in that, The edible formula oil obtained in S4 needs to be filtered through a multi-layer plant fiber filter with a filtering precision of 35-75 μm.

8. The health care formula oil of camellia oil, torreya grandis oil and walnut oil according to claim 4, characterized in that, The storage container of the edible formula oil is a light-proof brown glass bottle, and the storage temperature is 10-20℃, and the relative humidity is ≤65%.

9. The health care formula oil of camellia oil, torreya grandis oil and walnut oil according to claim 1, characterized in that, The perilla seed oil, the sea buckthorn fruit oil and the grape seed oil all need to be filtered through a 300-mesh filter cloth before use.

10. The health care formula of camellia oil, kaya oil and walnut oil according to claim 1, characterized in that, 5%-7% of the total mass percentage of the grape seed oil is composed of the grape seed oil directly added into the base oil in S2 and the grape seed oil used for dissolving the rosemary extract in S4, wherein the mass of the grape seed oil used for dissolving the rosemary extract in S4 accounts for 5%-15% of the total mass of the grape seed oil, and the mass of the grape seed oil directly added in S2 accounts for 85%-95% of the total mass of the grape seed oil.

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