A grinding device for extracting nucleic acid from mosquito vector samples

Abstract

The application discloses a grinding device for mosquito vector sample nucleic acid extraction, which comprises a shell and further comprises a rotating structure in the shell, wherein the rotating structure comprises a rotatable mounting disc, a plurality of grinding parts can be placed on the mounting disc, and the grinding part comprises a grinding tube and a movable grinding column which is located in the grinding tube and can be locked. The application solves the problems of insufficient and uneven sample grinding by "centrifugal force control sample + eccentric trajectory grinding", solves the problems of sample transfer pollution and complicated operation by "grinding-centrifugation integration + closed design", and simultaneously assists with low-temperature protection, multiple limiting, reliable sealing and other structures, so as to finally realize the goals of "sufficient and uniform grinding, simple and efficient operation, less sample pollution and high nucleic acid quality" in the process of mosquito vector sample nucleic acid extraction, and greatly optimizes the nucleic acid extraction process.

Description

Technical Field

[0001] This invention relates to the field of mosquito nucleic acid extraction and grinding technology, and in particular to a grinding device for extracting nucleic acid from mosquito vector samples. Background Technology

[0002] Currently, nucleic acid extraction from mosquito vector samples typically requires grinding. However, existing grinding devices generally consist of a grinding pestle and a grinding mortar. During grinding, mosquito vector samples tend to pool at the edges of the grinding area due to the grinding and squeezing action, preventing thorough grinding and resulting in incomplete grinding. This necessitates manual repositioning of the sample from the edges to the center of the grinding area, severely impacting grinding efficiency, increasing operational difficulty, and failing to guarantee uniformity and consistency in grinding.

[0003] Secondly, after grinding mosquito vector samples, centrifugation is required. Transferring the ground samples to a centrifuge increases the risk of contamination and adds to the operational steps. Currently, there are very few devices that combine grinding and centrifugation in one unit, eliminating the need for subsequent transfer.

[0004] Therefore, this application proposes a grinding device for extracting nucleic acid from mosquito vector samples. Summary of the Invention

[0005] The purpose of this invention is to solve the above-mentioned technical problems by providing a grinding device for extracting nucleic acid from mosquito vector samples.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A grinding device for extracting nucleic acid from mosquito vector samples, comprising a housing, and further comprising,

[0008] A rotating structure located within a housing, the rotating structure including a rotatable mounting plate on which multiple grinding parts can be placed, the grinding parts including a grinding tube and a movable grinding column located inside the grinding tube and lockable;

[0009] It also includes an eccentric guide part that cooperates with the movable grinding column. The eccentric guide part enables the movable grinding column to move in a fixed direction perpendicular to the axis of the grinding tube. When the mounting plate drives the movable grinding column to rotate counterclockwise, the movable grinding column is in an unlocked state. Due to centrifugal force, it moves towards the bottom of the grinding tube, and the line contact of the movable grinding column intermittently abuts against the grinding tube, forming a non-single-center grinding trajectory. The rotation of the movable grinding column cooperates with the grinding tube to grind the mosquito vector sample. When the mounting plate reverses, the movable grinding column is in a locked state, and the mounting plate drives the grinding part to centrifuge and extract nucleic acid from the ground mosquito vector sample.

[0010] Preferably, the grinding part further includes a piston block and a liquid storage tube that passes through the piston block and is fixedly connected to it. The piston block has a mounting hole in the middle, and a hollow rubber ring is installed in the mounting hole. The liquid storage tube is located inside the hollow rubber ring and is fixedly connected. The movable grinding column has a receiving cavity inside, and the piston block is movably connected in the receiving cavity. The piston block and the movable grinding column are provided with reset members for the movable grinding column to reset.

[0011] The upper inner wall of the grinding tube is provided with a sealing part, and the liquid storage tube passes through the sealing part and is rotatably connected to it.

[0012] Preferably, the eccentric guide portion is disposed in the receiving cavity, and the eccentric guide portion includes a guide block disposed in the receiving cavity. A rectangular sleeve is movably disposed on the guide block, and the rectangular sleeve is fitted onto the liquid storage tube and fixedly connected to it.

[0013] It also includes two movable parts located at both ends of the guide block, with the ends of the movable parts facing away from the guide block connected to the inner sidewall of the storage cavity.

[0014] Preferably, the device also includes a sealing switch for locking the movable grinding column. When grinding the mosquito vector sample, the sealing switch is in the open state, and the movable grinding column can move due to centrifugal force. When centrifuging the nucleic acid of the ground mosquito vector sample, the sealing switch is closed, and the movable grinding column is locked.

[0015] Preferably, the sealing switch includes an arc-shaped block fixed to the opening of the liquid storage tube and sealed therewith. The upper end of the arc-shaped block is provided with a sealing block that abuts against it. The sealing block rotates about the axis of the liquid storage tube. A pressure equalization hole is provided through the sealing block, and a locking block is fixed at the bottom of the sealing block.

[0016] Preferably, the mounting plate has multiple placement holes arranged in a circular array, the grinding tube is placed in the placement holes, the upper end of the mounting plate has a protrusion, and the upper end of the protrusion has a locking groove.

[0017] Preferably, it further includes a limiting mechanism for limiting the grinding tube, the limiting mechanism including a positioning plate, a pressure plate rotatably connected to the bottom of the positioning plate, the pressure plate being able to limit the sealing part, a limiting protrusion fixed to the bottom of the pressure plate, the limiting protrusion being configured to cooperate with the locking groove.

[0018] Preferably, a toothed ring is fixed to the bottom of the positioning disk, a transmission gear is rotatably connected to the upper outer wall of the liquid storage tube, and a sealing block is fixed on the transmission gear. When the limiting protrusion and the locking groove are engaged, the transmission gear meshes with the toothed ring.

[0019] Preferably, the upper end of the positioning disk is provided with a columnar protrusion, the upper end of the columnar protrusion is recessed and provided with a polygonal groove, a limiting plate is placed on the housing, the limiting plate is provided with a polygonal prism, and the polygonal prism can be inserted into the polygonal groove.

[0020] Preferably, a sealing cover is hinged to the housing, and when the sealing cover is closed on the housing, the sealing cover limits the position of the limiting plate.

[0021] Compared with the prior art, the beneficial effects of this invention are as follows:

[0022] 1. During the grinding process, the mounting plate drives the grinding section to rotate, generating centrifugal force. This keeps the mosquito vector sample within the core grinding area at the bottom of the grinding tube, preventing the sample from pooling towards the edges due to grinding pressure. Even if a small amount of sample splashes, it will return to the bottom under centrifugal force, eliminating the need for manual collection by the operator. This fundamentally solves the problem of sample dispersion in traditional grinding and ensures thorough grinding.

[0023] 2. Through the cooperation of the eccentric guide (guide block, rectangular sleeve, movable parts, etc.) and the movable grinding column, the movable grinding column rotates while being subjected to different centrifugal forces due to the varying angles of the movable parts, forming a non-single-center grinding trajectory. Furthermore, the gap and counter-pressure between the movable grinding column and the grinding tube dynamically change with rotation, enabling multi-directional grinding of the sample, significantly improving grinding uniformity and consistency, and avoiding the problem of incomplete local grinding that exists in traditional single-trajectory grinding.

[0024] 3. The function is switched by the forward and reverse rotation of the stepper motor. When rotating forward, the moving grinding column is driven to complete the grinding. When rotating in reverse, the moving grinding column is automatically locked, and the mounting plate directly drives the grinding tube for centrifugation. Throughout the process, the sample remains in the sealed grinding tube, eliminating the need to transfer the ground sample to external centrifuge equipment. This completely reduces the chance of the sample coming into contact with the outside environment during transfer and significantly reduces the risk of contamination.

[0025] 4. During centrifugation, the movable grinding column is locked in a closed state by a sealing switch (a combination of a sealing block, a locking block, and an arc-shaped block), retracting into the grinding tube and preventing contact with the sample. This prevents the movable grinding column from shaking and interfering with sample stratification during centrifugation, ensuring the centrifugation effect. It also prevents sample from adhering to the surface of the movable grinding column, reducing sample waste, and avoids contamination of the supernatant by the movable grinding column during subsequent sampling, ensuring the quality of nucleic acid extraction.

[0026] 5. The integrated design eliminates the traditional steps of "removing the ground sample - transferring it to a centrifuge tube - installing the centrifuge tube". Operators only need to control the stepper motor to switch between grinding and centrifugation, which greatly reduces the number of operation steps, reduces the complexity of operation, avoids time loss during the transfer process, and significantly improves the overall efficiency of nucleic acid extraction.

[0027] In summary, this invention addresses the core pain points of traditional grinding equipment by solving the problems of insufficient and uneven sample grinding through "centrifugal force-controlled sample grinding + eccentric trajectory grinding," and by solving the problems of sample transfer contamination and cumbersome operation through "integrated grinding-centrifugation + sealed design." Furthermore, it incorporates low-temperature protection, multiple limiting mechanisms, and reliable sealing structures, ultimately achieving the goals of "thorough and uniform grinding, simple and efficient operation, low sample contamination, and high nucleic acid quality" in the nucleic acid extraction process of mosquito vector samples, significantly optimizing the nucleic acid extraction process. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the structure of a grinding device for extracting nucleic acid from mosquito vector samples proposed in this invention;

[0029] Figure 2 This is a split view of a grinding device for extracting nucleic acid from mosquito vector samples according to the present invention;

[0030] Figure 3 This is a schematic diagram of the disassembled positioning disk in a grinding device for extracting nucleic acid from mosquito vector samples proposed in this invention;

[0031] Figure 4 This is a schematic diagram of the mounting plate in a grinding device for extracting nucleic acid from mosquito vector samples according to the present invention;

[0032] Figure 5 This is a schematic diagram of the structure of the grinding section after loading in the grinding device for extracting nucleic acid from mosquito vector samples proposed in this invention;

[0033] Figure 6 This is a bottom view of the positioning plate in a grinding device for extracting nucleic acid from mosquito vector samples according to the present invention;

[0034] Figure 7 This is a cross-sectional view of the grinding section in a grinding device for extracting nucleic acid from mosquito vector samples according to the present invention;

[0035] Figure 8 This is a schematic diagram of the grinding section in a grinding device for extracting nucleic acid from mosquito vector samples proposed in this invention;

[0036] Figure 9 This is a schematic diagram showing the disassembled grinding section of a grinding device for extracting nucleic acid from mosquito vector samples proposed in this invention;

[0037] Figure 10 This is a schematic diagram of the internal structure of the grinding tube in a grinding device for extracting nucleic acid from mosquito vector samples proposed in this invention;

[0038] Figure 11This is a schematic diagram of the disassembled sealing switch in a grinding device for extracting nucleic acid from mosquito vector samples proposed in this invention;

[0039] Figure 12 This is a diagram showing the opening and closing state of the sealing switch in a grinding device for extracting nucleic acid from mosquito vector samples proposed in this invention.

[0040] Figure 13 This is a cross-sectional view of the movable grinding column in a grinding device for extracting nucleic acid from mosquito vector samples according to the present invention.

[0041] In the diagram: 100, housing; 110, sealing cover; 200, refrigeration equipment; 300, rotating structure; 310, positioning plate; 311, columnar protrusion; 312, gear ring; 313, pressure plate; 314, limiting protrusion; 320, limiting plate; 321, polygonal prism; 330, mounting plate; 331, protrusion; 332, placement hole; 340, grinding part; 341, grinding tube; 342, sealing part; 343, transmission gear; 344, sealing switch; 3441, sealing block; 3442, pressure equalization hole; 3443, locking block; 3444, arc-shaped block; 345, movable grinding column; 346, liquid storage tube; 3461, rectangular sleeve; 3462, guide block; 3463, moving part; 347, piston block; 3471, hollow rubber ring; 348, reset part. Detailed Implementation

[0042] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0043] Reference Figures 1-13 A grinding device for extracting nucleic acid from mosquito vector samples, comprising a housing 100, and further comprising,

[0044] The rotating structure 300 is located inside the housing 100. The rotating structure 300 includes a rotatable mounting plate 330. Multiple grinding parts 340 can be placed on the mounting plate 330. Each grinding part 340 includes a grinding tube 341 and a movable grinding column 345 located inside the grinding tube 341 and lockable.

[0045] It also includes an eccentric guide part that cooperates with the movable grinding column 345. The eccentric guide part enables the movable grinding column 345 to move in a fixed direction perpendicular to the axis of the grinding tube 341. The grinding column 345 can also move in the radial direction of the grinding tube 341. When the mounting plate 330 drives the movable grinding column 345 to rotate counterclockwise, the movable grinding column 345 is in the unlocked state. Due to the centrifugal force, it moves towards the bottom of the grinding tube 341 and the movable grinding column 345 intermittently contacts the grinding tube 341 with line contact, forming a non-single-center grinding trajectory. The movable grinding column 345 rotates and cooperates with the grinding tube 341 to grind the mosquito vector sample. After grinding, the movable grinding column 345 is reset. When the mounting plate 330 reverses, the movable grinding column 345 is in the locked state. The mounting plate 330 drives the grinding part 340 to centrifuge and extract nucleic acid from the ground mosquito vector sample.

[0046] like Figure 2 , Figure 3 , Figure 4 As shown, the upper end of the positioning disk 310 is provided with a columnar protrusion 311, and the upper end of the columnar protrusion 311 is recessed with a polygonal groove. A limiting plate 320 is placed on the housing 100, and the housing 100 is recessed with two limiting grooves. The two ends of the limiting plate 320 are located in the limiting grooves, so that it is not easy to move horizontally. A polygonal prism 321 is provided on the limiting plate 320, and a polygonal hole is provided through the limiting plate 320. The upper end of the polygonal prism 321 has a round block larger than the polygonal hole to prevent the polygonal prism 321 from falling out through the polygonal hole. The polygonal prism 321 can be inserted into the polygonal groove, so as to limit the positioning disk 310 and prevent it from rotating.

[0047] like Figure 1 , Figure 2 As shown, a sealing cover 110 is hinged to the housing 100. When the sealing cover 110 is closed on the housing 100, the sealing cover 110 limits the limiting plate 320. A refrigeration device 200 is also installed on one side of the housing 100 to provide a low-temperature environment for grinding and centrifugation, inhibiting nuclease activity and reducing the risk of nucleic acid degradation. Secondly, the low temperature makes mosquito tissues such as chitinous exoskeleton and muscles brittle, reducing grinding resistance and improving crushing efficiency.

[0048] like Figure 4 As shown, the mounting plate 330 has multiple placement holes 332 through it. The placement holes are inclined. The inner wall of the placement hole 332 can be provided with a sliding groove. The grinding tube 341 is placed in the placement hole 332. The outer wall of the grinding tube 341 is provided with a sliding piece. The sliding piece is located in the sliding groove and can limit the grinding tube 341 to ensure that the grinding tube 341 will not rotate. The multiple placement holes 332 are arranged in a ring array on the mounting plate 330. The upper end of the mounting plate 330 is provided with a protrusion 331. The upper end of the protrusion 331 is recessed and provided with a locking groove.

[0049] like Figure 6 As shown, it also includes a limiting mechanism for limiting the grinding tube 341. The limiting mechanism includes a positioning plate 310, and a pressure plate 313 is rotatably connected to the bottom of the positioning plate 310. The pressure plate 313 can limit the sealing part 342, thus limiting the grinding tube 341 in the centrifuge. A limiting protrusion 314 is fixed at the bottom of the pressure plate 313. The limiting protrusion 314 is configured to cooperate with the locking groove, so that the pressure plate 313 will rotate with the mounting plate 330, and can always limit the grinding tube 341. A stepper motor is provided at the bottom of the housing 100, and the output end of the stepper motor is coaxially fixedly connected to the mounting plate 330.

[0050] like Figure 8 , Figure 9 , Figure 10 As shown, the grinding part 340 also includes a piston block 347 and a liquid storage tube 346 that passes through the piston block 347 and is fixedly connected to it. The piston block 347 has a mounting hole in the middle, and a hollow rubber ring 3471 is installed in the mounting hole. The liquid storage tube 346 is located inside the hollow rubber ring 3471 and is fixedly connected. A telescopic rod can be installed inside the hollow rubber ring 3471 so that the liquid storage tube 346 is not easy to move relative to the piston block 347 in the axial direction.

[0051] The movable grinding column 345 has a receiving cavity inside, which connects the liquid storage tube 346 to the receiving cavity. The end of the movable grinding column 345 is spherical. The liquid storage tube 346 and the receiving cavity store hydraulic oil, water, or other oil, or air. The piston block 347 is movably connected in the receiving cavity. The piston block 347 and the movable grinding column 345 are provided with a reset member 348 for resetting the movable grinding column 345. The reset member 348 can be a spring or other structure that enables the movable grinding column 345 to reset.

[0052] like Figure 9 , Figure 11 As shown, the upper inner wall of the grinding tube 341 is provided with a sealing part 342, and the liquid storage tube 346 passes through the sealing part 342 and is rotatably connected to it. The sealing part 342 can be a rubber sealing plug, which supports the liquid storage tube 346. The inner wall of the grinding tube 341 can be provided with protrusions or grooves, and the corresponding sealing part 342 is provided with matching protrusions or grooves. When the two are engaged, the sealing block 3441 is in its initial position. That is, all sealing blocks 3441 are in their initial position after installation. If any sealing block 3441 rotates, the transmission gear 343 can be rotated to bring it to its initial position. Figure 12 The position shown in the left figure; to ensure stable rotation of the liquid storage tube 346, a bearing can be installed through the middle of the sealing part 342, with the liquid storage tube 346 passing through the inner ring of the bearing with an interference fit.

[0053] like Figure 7 , Figure 10As shown, the eccentric guide is disposed in the receiving cavity. The eccentric guide includes a guide block 3462 disposed in the receiving cavity. A rectangular sleeve 3461 is movably disposed on the guide block 3462. A rectangular groove passes through the guide block 3462. The rectangular sleeve 3461 is slidably connected in the rectangular groove, so as to ensure that the two rotate synchronously. The rectangular sleeve 3461 is fitted on the liquid storage tube 346 and fixedly connected to it.

[0054] like Figure 10 As shown, it also includes two movable parts 3463, located at both ends of the guide block 3462. The end of the movable part 3463 facing away from the guide block 3462 is connected to the inner side wall of the storage cavity. The movable part 3463 is fixedly connected to the guide block 3462 and the inner wall of the storage cavity. The movable part 3463 can be an airbag, but a guide rail slider or telescopic rod needs to be added inside. The purpose is to fix the guide block 3462 relatively in the storage cavity through the movable parts 3463, and it can only move back and forth in the space between the two movable parts 3463.

[0055] Figures 8-12 As shown, the sealing switch 344 includes an arc-shaped block 3444 fixed to and sealed at the opening of the liquid storage tube 346. A sealing block 3441 abuts against the upper end of the arc-shaped block 3444. The sealing block 3441 is circular with a diameter larger than the outer diameter of the liquid storage tube 346, ensuring complete coverage. The sealing block 3441 rotates about the axis of the liquid storage tube 346. A sealing element is provided at the end of the liquid storage tube 346 and slides against the sealing block 3441. That is, when the sealing block 3441 moves, the two elements are always against each other and in a sealed state. The sealing element can be made of rubber. Alternatively, a sealing ring or other sealing method may be used; a pressure equalization hole 3442 is provided through the sealing block 3441, wherein a small sealing ring is provided around the periphery of the pressure equalization hole 3442 at the bottom of the sealing block 3441. When the pressure equalization hole 3442 is opposite to the arc-shaped block 3444, the small sealing ring abuts against the upper end of the arc-shaped block 3444, thus sealing the pressure equalization hole 3442 separately; and a locking block 3443 is fixed at the bottom of the sealing block 3441, the lower end of the locking block 3443 being lower than the lower end of the arc-shaped block 3444, ensuring that the locking block 3443 can abut against the arc-shaped block 3444.

[0056] Reference Figure 12 When grinding mosquito vector samples, the sealing switch 344 is in the open state, and the movable grinding column 345 can move due to centrifugal force; when centrifuging the nucleic acid of the ground mosquito vector sample, the sealing switch 344 is closed, and the movable grinding column 345 is locked; when in the open state, such as Figure 12In the left figure, the locking block 3443 is abutting against the arc block 3444. If the sealing block 3441 rotates counterclockwise, it will drive the arc block 3444 and the liquid storage tube 346 to rotate. It should be noted that the equalizing hole 3442 is not opposite to the arc block 3444 at this time.

[0057] If the sealing block 3441 rotates clockwise, it will cause the locking block 3443 to rotate. As the sealing block 3441 and locking block 3443 rotate, the locking block 3443 eventually abuts against the other sidewall of the arc-shaped block 3444. At this point, the pressure equalizing hole 3442 is aligned with the arc-shaped block 3444. Figure 12 As shown in the right figure; at this time, the opening of the liquid storage tube 346 is in a sealed state, thus the movable grinding column 345 is in a locked state.

[0058] like Figure 7 , Figure 9 , Figure 10 As shown, a gear ring 312 is fixed to the bottom of the positioning disk 310, and a transmission gear 343 is rotatably connected to the upper outer wall of the liquid storage tube 346. The transmission gear 343 is rotatably mounted on the liquid storage tube 346 through a bearing. The liquid storage tube 346 is made of stainless steel. The sealing block 3441 is fixed on the transmission gear 343. Therefore, when the transmission gear 343 rotates, it drives the outer ring of the bearing to rotate, which can drive the sealing block 3441 to rotate. When the limiting protrusion 314 is engaged with the locking groove, the transmission gear 343 meshes with the gear ring 312.

[0059] In use, open the sealing cover 110, then remove the polygonal prism 321, the limiting plate 320, and the positioning plate 310. Add the mosquito vector sample and lysis fluid such as Trizol into the grinding tube 341. Then, seal the grinding tube 341 with the sealing part 342 and place the sealed grinding part 340 into the placement hole 332. Next, fasten the limiting protrusion 314 at the lower end of the positioning plate 310 into the locking groove at the upper end of the protrusion 331. At this time, the gear ring 312 and the transmission gear 343 are engaged. Then, install the limiting plate 320 and insert the polygonal prism 321 into the polygonal groove at the upper end of the columnar protrusion. Cover the sealing cover 110 to complete the preparation.

[0060] Then, the cooling equipment 200 is activated to provide a low-temperature environment for the grinding section 340; the stepper motor is activated to drive the mounting plate 330 to rotate counterclockwise. Figure 12 The left view shows the forward rotation; the rotation of the mounting plate 330 drives the grinding part 340 to rotate. Since the equalizing hole 3442 is not opposite to the arc block 3444 at this time, that is, the liquid storage tube 346 is connected to the outside, the grinding part 340 will generate centrifugal force when it rotates. When the centrifugal force on the movable grinding column 345 is greater than the elastic force of the reset member 348, the movable grinding column 345 moves along the length of the grinding tube 341. Finally, the end of the movable grinding column 345 abuts against the inner bottom of the grinding tube 341.

[0061] The moving grinding column 345 moves downward and moves relative to the piston block 347, which can draw the liquid inside the liquid storage tube 346 into the receiving cavity.

[0062] Since the gear ring 312 cannot rotate, when the mounting plate 330 drives the grinding part 340 to rotate, the transmission gear 343 rolls on the gear ring 312. The rotation of the transmission gear 343 drives the sealing switch 344 to rotate in its initial state, which in turn drives the liquid storage tube 346 to rotate. The rotation of the liquid storage tube 346 drives the rectangular sleeve 3461, the guide block 3462, the movable part 3463, and the movable grinding column 345 to rotate. Since the grinding tube 341 does not rotate, there is a relative rotation between the movable grinding column 345 and the grinding tube 341. The sample can be ground by the movable grinding column 345.

[0063] It should be noted that, due to the centrifugal force, the sample to be ground is always at the bottom of the grinding tube 341. Therefore, even if there is any splashing when the moving grinding column 345 grinds it, it will return to the bottom of the grinding tube 341 due to the centrifugal force, thus ensuring that the sample is fully ground.

[0064] When the transmission gear 343 drives the grinding part 340 to rotate to the position shown in the figure Figure 13 When the position is correct, that is, the centrifugal force can be effectively applied to the movable part 3463. Specifically, because the movable grinding column 345 is subjected to centrifugal force, it will drive the reset part 348 and the piston block 347 to move, which will squeeze the movable part 3463 and the hollow rubber ring 3471 to the right. As a result, the movable grinding column 345 will be slightly offset to the left. The liquid storage tube 346 will drive the movable grinding column 345 to rotate eccentrically, thus increasing the pressure between the movable grinding column 345 and the grinding tube 341, so that the sample can be better ground and the grinding is a line contact.

[0065] As the movable part 3463 rotates, due to the centrifugal force, the movable part 3463 at different angles experiences different pressures. Therefore, the gap between the movable grinding column 345 and the grinding tube 341 also changes, and the pressure after they come into contact changes accordingly. This causes the movable grinding column 345 to form a non-single-center grinding trajectory, resulting in a better grinding effect on the sample.

[0066] It should be noted that the outer wall of the movable grinding column 345 is positioned close to the inner wall of the grinding tube 341, but is not always in close contact. Thus, when the movable grinding column 345 comes into contact with the bottom of the grinding tube 341, it will squeeze the mosquito vector sample and lysis solution, causing the sample and lysis solution to flow into this gap, which can efficiently and thoroughly grind them.

[0067] After grinding is completed, the stepper motor stops working, and the movable grinding column 345 is reset under the action of the reset component 348.

[0068] Then, the stepper motor is started to reverse its direction, i.e., rotate clockwise. The transmission gear 343 drives the sealing block 3441 to rotate, and the sealing block 3441 drives the locking block 3443 to rotate. As the sealing block 3441 and the locking block 3443 rotate, the locking block 3443 eventually abuts against the other side wall of the arc-shaped block 3444. At this time, the pressure equalizing hole 3442 is opposite to the arc-shaped block 3444. Figure 12 As shown in the right figure; at this time, the opening of the liquid storage tube 346 is in a sealed state, so the movable grinding column 345 is in a locked state. Therefore, the receiving cavity and the inside of the liquid storage tube 346 are in a sealed state.

[0069] The stepper motor drives the mounting plate and the grinding tube 341 to rotate, which can centrifuge the ground sample. Due to the centrifugal force, the sample and lysis solution on the moving grinding column 345 will be thrown off and mixed with the sample in the grinding tube 341, reducing sample waste. Finally, after centrifugation, the supernatant in the grinding tube 341 can be taken.

[0070] Because the end of the liquid storage tube 346 is sealed, even under centrifugal force, the movable grinding column 345 is in a retracted state and will not come into contact with the sample, thus avoiding any impact on subsequent sample collection.

[0071] In this way, grinding and centrifugation are combined on the same equipment, eliminating the need for transfer, which improves extraction efficiency and reduces contamination.

[0072] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A grinding device for extracting nucleic acid from a mosquito vector sample, comprising a housing (100), characterized in that, Also include, The rotating structure (300) is located in the shell (100), the rotating structure (300) includes the rotatable mounting disc (330), a plurality of grinding parts (340) can be placed on the mounting disc (330), the grinding part (340) includes a grinding pipe (341) and a movable grinding column (345) located in the grinding pipe (341) and can be locked; Also include the eccentric guide part matched with the movable grinding column (345), the eccentric guide part can make the movable grinding column (345) move along the grinding pipe (341) perpendicular to the axis fixed direction, when the mounting disc (330) drives the movable grinding column (345) to rotate counterclockwise, the movable grinding column (345) is in the unlocked state, and the movable grinding column (345) is moved towards the bottom of the grinding pipe (341) due to the centrifugal force and intermittently abuts against the grinding pipe (341) in the form of line contact, a non-single center grinding track is formed, the movable grinding column (345) rotates to cooperate with the grinding pipe (341) to grind mosquito vector samples; The mounting disc (330) is reversed, the movable grinding column (345) is in the locked state, and the mounting disc (330) drives the grinding part (340) to extract the nucleic acid of the ground mosquito vector sample by centrifugation; The grinding part (340) further includes a piston block (347) and a liquid storage pipe (346) penetrating through the piston block (347) and fixedly connected with the piston block (347), the inside of the movable grinding column (345) is provided with a receiving cavity, the eccentric guide part is arranged in the receiving cavity, the eccentric guide part includes a guide block (3462) arranged in the receiving cavity, a rectangular sleeve (3461) is movably arranged on the guide block (3462), and the rectangular sleeve (3461) is sleeved on the liquid storage pipe (346) and fixedly connected with the liquid storage pipe (346); Further include the movable element (3463), one end of the movable element (3463) away from the guide block (3462) is connected with the inner side wall of the receiving cavity; 2. The grinding device for nucleic acid extraction of a mosquito vector sample according to claim 1, wherein Further include a sealing switch (344) for locking the movable grinding column (345), the sealing switch (344) includes an arc-shaped block (3444) fixedly arranged on the liquid storage pipe (346) and sealingly arranged, the upper end of the arc-shaped block (3444) is provided with a sealing block (3441) abutting against the arc-shaped block (3444), the sealing block (3441) rotates around the axis of the liquid storage pipe (346), the sealing block (3441) is provided with an equalizing hole (3442) penetrating through the sealing block (3441), and the bottom of the sealing block (3441) is fixedly provided with a locking block (3443). The piston block (347) is movably connected in the receiving cavity, and the piston block (347) is provided with a reset element (348) for resetting the movable grinding column (345) on the movable grinding column (345); The inner wall of the upper end of the grinding pipe (341) is provided with a sealing part (342), and the liquid storage pipe (346) penetrates through the sealing part (342) and is rotatably connected with the sealing part (342).

3. The grinding device for nucleic acid extraction of a mosquito vector sample according to claim 2, characterized by The movable part (3463) is two, and is located at both ends of the guide block (3462).

4. The grinding device for nucleic acid extraction of a mosquito vector sample according to claim 3, characterized by When the mosquito vector sample is ground, the sealing switch (344) is in the open state, and the movable grinding column (345) can move due to centrifugal force; when the nucleic acid of the ground mosquito vector sample is centrifuged, the sealing switch (344) is closed, and the movable grinding column (345) is locked.

5. The grinding device for nucleic acid extraction of mosquito vectors according to claim 1, wherein A plurality of placement holes (332) are provided through the mounting disc (330), the plurality of placement holes (332) are arranged in a ring array on the mounting disc (330), the grinding pipe (341) is placed in the placement hole (332), the upper end of the mounting disc (330) is provided with a protrusion (331), and the upper end of the protrusion (331) is recessed to be provided with a locking groove.

6. The grinding device for nucleic acid extraction of a mosquito vector sample according to claim 5, wherein The limiting mechanism for limiting the grinding pipe (341) includes a positioning disc (310), the bottom of the positioning disc (310) is rotatably connected with a pressing disc (313), the pressing disc (313) can limit the sealing part (342), the bottom of the pressing disc (313) is fixed with a limiting protrusion (314), and the limiting protrusion (314) is matched with the locking groove.

7. The grinding device for nucleic acid extraction from mosquito vectors according to claim 6, wherein The bottom of the positioning disc (310) is fixed with a tooth ring (312), the upper end outer wall of the liquid storage pipe (346) is rotatably connected with a transmission gear (343), a sealing block (3441) is fixed on the transmission gear (343), and when the limiting protrusion (314) is matched with the locking groove, the transmission gear (343) is engaged with the tooth ring (312).

8. The grinding device for nucleic acid extraction of a mosquito vector sample according to claim 6, wherein The upper end of the positioning disc (310) is provided with a columnar protrusion (311), the upper end of the columnar protrusion (311) is recessed to be provided with a multi-rib groove, the shell (100) is placed with a limiting plate (320), the limiting plate (320) is provided with a multi-rib column (321), and the multi-rib column (321) can be inserted into the multi-rib groove.

9. The grinding device for nucleic acid extraction from a mosquito vector sample according to claim 8, wherein The shell (100) is hingedly connected with a sealing cover (110), when the sealing cover (110) is closed on the shell (100), the sealing cover (110) limits the limiting plate (320).

Images